Cytoplasm type dividing method of millet breeding materials

Abstract

The invention discloses a cytoplasm type dividing method of millet breeding materials. The cytoplasm type dividing method is characterized by comprising the following steps of (1) cytoplasm source evolutionary tree building; (2) female parent derivation evolution chart building; (3) specific genetic similarity coefficient determination and cytoplasm source molecular clustering; and (4) cytoplasm type determination of the millet breeding materials. The method has the advantages that the foundation is laid for culturing the sterile system and the hybrid seed of different cytoplasm types, and the foundation is laid for creating the millet sterile system of different cytoplasm types and culturing the millet hybrid seed of different cytoplasm types.

Description

technical field [0001] The invention relates to a method for classifying types of millet breeding materials, in particular to a method for classifying cytoplasm types of millet breeding materials, and belongs to the field of crop variety classification research. Background technique [0002] The traditional classification method of millet breeding materials is based on a certain aspect of the breeding materials. For example, according to the fertility, it can be divided into normal fertile materials and male sterile lines, and according to the plant type, it can be divided into compact, semi The compact type and the diffuse type are divided into spring valley and summer valley according to the suitable planting season, and are divided into early maturity, middle maturity and late maturity according to the growth period. [0003] In recent years, with the development of biotechnology, scholars have studied the phylogenetic relationship between millet germplasm resources by co...

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Problems solved by technology

[0003] In recent years, with the development of biotechnology, scholars have studied the phylogenetic relationship between millet germplasm resources by constructing evolutionary trees, and based on this study the genetic evolution relationship of germplasm resources, and then studied the reports on the relationship between various groups, ecological types, and geographical sources , such as Wang Jiezhi in 2006 (Biotechnology, 2006, 16(1): 10-14) used SSR primers to study the genetic evolution relationship of 96 millet varieties resources from different ecological regions in the country by constructing phylogenetic trees. Breeding materials are divided into 5 groups, but no conclusion can be drawn that the genetic groups are consistent with the ecological types

In 2009, Jia Xiaoping (Theor Appl Genet, 2009, 118: 821-829) also used phylogenetic trees to study the genetic evolution of millet germplasm resources. The genetic similarity coefficient of tree longitudinal section was determined to be 0.77 or 0.8. When the genetic similarity coefficient was 0.77, the test materials were divided into 2 groups; when the genetic similarity coefficient was 0.8, the test materials were divided into 5 groups. Unable to determine specific genetic similarity coefficients for vertically slicing evolutionary trees, and therefore, cannot draw ideal results for genetic evolution studies

And all these studies are carried out based on the genetic information of the nucleus. So far, there is no report on the classification of millet breeding materials based on the cytoplasmic genetic information.

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