1882results about "Microorganisms" patented technology

The invention relates to the technical field of agricultural biology, and discloses an auxiliary cultivation method for promoting sugarcane biological nitrogen fixation, which comprises the following steps: (1) breeding a sugarcane variety with high nitrogen-fixing bacteria affinity, and preparing an environmental stress resistant nitrogen-fixing bacteria agent; (2) carrying out acidification improvement and carbon source pre-burying on the planting soil; (3) inoculating the nitrogen-fixing bacterial agent to the sugarcane seed stem or rhizosphere area; (4) maintaining the metabolic activity of nitrogen-fixing bacteria by dynamically regulating and controlling rhizosphere carbon source supply and oxygen partial pressure; (5) adjusting a fungicide supplementary application strategy in real time based on the environmental data; and (6) solidifying the symbiotic character through intergenerational epigenetic treatment. Through host-microorganism coevolution and intelligent dynamic regulation and control, the nitrogen fixation efficiency and environmental adaptability of the sugarcane-nitrogen-fixing bacteria symbiotic system are remarkably improved; and epigenetic and flora iteration technologies are combined, so that stable transmission of cross-generation characters is realized, and a low-consumption and high-benefit ecological solution is provided for sustainable agriculture.

To provide a technology that balances increased food production and control of environmental pollution that can cope with the increasing population.SOLUTION: After the agrochemicals used on the crops fulfilled their function, the ascomycetes white wood-rotting fungus having the characteristics of mycorrhizal fungus is used to decompose the agrochemicals remaining on the crops and soil.SELECTED DRAWING: Figure 4

A technique related to codon optimization is provided. In a method for optimizing nucleic acid sequences expressing proteins in a host, protein subsequence-nucleic acid subsequence pairs are obtained from collected highly expressed protein sequences and their encoding nucleic acid sequences to form a training set. And training a neural machine translation model by using the training set. The neural machine translation model realizes translation from an amino acid sequence to a codon sequence. A protein sequence needing codon optimization is cut into protein subsequences. And translating a protein subsequence from an amino acid sequence to a codon sequence by using the trained neural machine translation model. The translated subsequences are superimposed to merge into a full-length codon sequence. In the process of superimposing to synthesize the codon sequence, according to the frequency or number of synonymous codons corresponding to each amino acid position of the protein sequence, the synonymous codon with the highest occurrence frequency or number is selected as the optimal codon at the position.

Provided are a strain of Lactobacillus plantarum, i.e., Lactobacillus plantarum PDG8, and compositions thereof. Also provided are methods of treating cardiovascular diseases, such as atherosclerosis, using Lactobacillus plantarum PDG8 or a composition thereof.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The subject invention provides compositions and methods for remediating and mitigating iron pollution. In preferred embodiments, a composition comprising an iron-capturing ingredient is contacted with an environment suffering from iron pollution in order to reduce the amount of iron in the environment.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The invention discloses a bacterial inhibitor for sea bass preservation, the bacterial inhibitor is bacteriocin generated by fermentation of lactobacillus plantarum L13 (the preservation number is CGMCC NO.27398), the bacteriocin comprises active polypeptide xylAB, the amino acid sequence of the xylAB is LLSSLATTGAIALGATAAKA, the preservation number of the bacterial inhibitor is CGMCC NO.27398, the preservation number of the bacterial inhibitor is CGMCC NO.27398, and the preservation number of the bacterial inhibitor is CGMCC NO.27398. The fresh-keeping agent prepared by the invention has a good fresh-keeping effect, can prolong the fresh-keeping period, and has huge potential as a natural food preservative in the food industry.

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

Provided herein are binding molecules comprising a human neonatal Fc receptor (FcRn) binding molecule and a moiety that does not specifically bind to a human antigen. Polynucleotides, vectors, host cells, and methods of production are also provided herein. Methods of treating an antibody-mediated disorder with an FcRn binding molecule or an FcRn / antigen-binding molecule are further provided.

The invention relates to a method for evaluating and debugging a sterile pipeline for food and beverage processing, which comprises the following steps of: introducing standardized sterile gas into a closed pipeline system to establish a micro-positive pressure environment; detecting a pressure change curve of each node of the system in real time by using a micro-differential pressure sensing module; calculating the position and the size of a trace leakage point; recording the motion trail of the particles in the pipeline by using a dynamic particle imaging system PIV; flow dead angles are identified through trajectory analysis, and the potential pollution accumulation area of the retention area marks the pollution accumulation probability of each section of pipeline in a quantitative mode; preparing simulation liquid, and adding biomarker particles to enable the viscosity and the fluidity of the target beverage to be close to those of the target beverage; according to the identified potential pollution accumulation area, local directional pouring is carried out; full-process automatic data acquisition, model judgment and debugging instruction issuing can be realized, manual intervention is greatly reduced, the system stability is improved, and the system is suitable for various food and beverage production scenes.

Stem cells can be cultured by suspension culture while improving functionalities of the stem cells by treating the stem cells with a water-soluble polymer (for example, culturing in the presence of the water-soluble polymer). Such a method is a more versatile culture method with low cost.

The invention relates to the technical field of aquaculture, and particularly discloses paracoccus pantotrophus P.p-O8 and application of the paracoccus pantotrophus P.p-O8, the paracoccus pantotrophus P.p-O8 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the paracoccus pantotrophus P.p-O8 is CGMCC NO.26522. The invention relates to a microbial inoculum prepared from paracoccus pantotrophus P.p-O8. The method has a huge application prospect in improving the quality of aquaculture water and repairing the polluted sediments in the cage culture area, improves the water environment quality and the survival conditions of aquaculture organisms, repairs the eutrophicated polluted ecological environment, and has high environmental protection value and economic benefit.

The invention discloses a deep-sea microorganism in-situ directional culture device, and relates to the technical field of deep-sea equipment, the deep-sea microorganism in-situ directional culture device comprises a microorganism culture cabin, a nutrient solution storage module, a bidirectional flow path assembly, an intelligent control module, a support assembly and the like; wherein the microorganism culture cabin consists of a flow channel, a flow choking block, a culture chamber, a movable small plate, a cabin cover and a sealing element, and the flow channel and the flow choking block form a structure based on a Tesla valve principle; through a bidirectional flow path assembly composed of a deep-sea high-precision metering pump, a deep-sea flow guide pump and the like, a nutrient substrate is injected in a reverse high-resistance mode to achieve efficient mixing, and seawater flows in a forward low-resistance mode to achieve environmental substance exchange; the deep-sea multi-channel switching valve is independently connected with the multiple sets of nutrient solution storage modules and the microorganism culture cabin, so that culture under various environmental conditions is realized; based on sensor data calculation and analysis, the starting and stopping time of the pump is intelligently controlled, the concentration of nutrient substrates in the microorganism culture cabin and the stability of environmental parameters are effectively guaranteed, and therefore in-situ culture deep-sea microorganism communities are controllable.

The invention discloses a process for repairing damaged soil ecology by lincomycin waste liquid fertilizer, and relates to the technical field of soil remediation, which comprises the following specific steps: step 1, screening and culturing microbial decomposition flora, the invention has the following advantages: through the chelation of the polyaspartic acid derivative on metal ions and the promotion effect on nutrient absorption, the release and transmission of nutrients are precisely regulated and controlled, so that crops can absorb and utilize the nutrients more efficiently; the nano composite material and the specific biological stimulant containing various beneficial microbial strains and natural plant extracts are jointly fused into the liquid fertilizer, the microbial strains in the specific biological stimulant activate the soil biological environment, the natural plant extracts assist plant growth to resist diseases, and the nano composite material and the natural plant extracts have a synergistic effect, so that the liquid fertilizer has a good application prospect. Physicochemical properties and biological activity of soil are optimized, excellent soil micro-ecology is created for plant growth, healthy and strong growth of plants is promoted, and restoration of damaged soil is enhanced.

To provide an inhibitor for suppressing expression of an antibacterial peptide in brown planthopper and an application thereof.SOLUTION: A dsNlPCE4 inhibitor designed based on the NlPCE4 gene of the brown planthopper is obtained. The inhibitor, when applied to brown planthoppers inoculated on their body surface with Metarhizium anisopliae, significantly increases the mortality of the brown planthopper. The present invention combines two technical strategies for pest control, biocontrol fungi and RNA interference (RNAi), to provide a new target for brown planthopper control technology based on cooperative mediation of RNAi.SELECTED DRAWING: Figure 5

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The invention relates to the field of drilling in the petroleum industry, and discloses a composition for a microbial stabilizer, the microbial stabilizer and application thereof, and a water-based drilling fluid and application thereof. The composition contains a thermophilic strain, a nutrient solution for the thermophilic strain, urea and a calcium source, the preservation number of the thermophilic strain is 1K04190; the content mass ratio of the thermophilic strain to the nutrient solution for the thermophilic strain to the urea to the calcium source is 1: (2-10): (1-10): (1-4); the OD600 (optical density 600) of the thermophilic strain is 0.4 to 0.6. The microbial stabilizer obtained by adopting the composition for the microbial stabilizer can effectively generate CaCO3 precipitates with high mechanical strength, can play a role in adjusting the rheological property of drilling fluid after being applied to the drilling fluid, can reduce the leakage of the drilling fluid in the drilling work, plays a role in stabilizing a well wall, and has the advantages that the drilling fluid is prevented from being damaged, and the service life of the drilling fluid is prolonged. Meanwhile, the mechanical strength of the rock can be obviously improved.

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

To provide a novel method for oil-water separation of an oil-in-water suspension and an oil-water separation device.SOLUTION: A method for oil-water separation is provided, comprising: contacting an oil-in-water suspension with water, which may contain microbubbles, such that a suspension-rich phase is positioned above and a water-rich phase is positioned below; supplying new microbubbles to the water-rich phase so as to prevent uniform mixing between the suspension-rich phase and the water-rich phase; and floating microbubbles having adsorbed micelles in the suspension. Also provided is an oil-water separation device comprising: a separation tank that houses an oil-in-water suspension and water, which may contain microbubbles, such that a suspension-rich phase is positioned above and a water-rich phase is positioned below; and a microbubble generator that generates microbubbles to be supplied into the separation tank, wherein the microbubble generator is disposed such that the generated microbubbles are supplied to the water-rich phase, and the interior of the separation tank is not stirred during microbubble generation in a manner of preventing uniformity inside the tank.SELECTED DRAWING: Figure 1

The subject invention provides compositions and methods for controlling iron-capturing pathogens. In preferred embodiments, the subject invention can be applied to an environment in which a pathological microorganism is growing, wherein the microorganism pathologically captures iron in order to control the pathogen by outcompeting the pathogen for iron resources.

The invention discloses a sulfonation modified metal organic framework material, a preparation method thereof and application of the sulfonation modified metal organic framework material in yield increase application of aryl glycoside compounds, and the sulfonation modified metal organic framework material takes a zirconium-based MOF material Zr-BTB as a precursor to react with 1, 2-ethanedisulfonic acid so as to carry out sulfonation modification. According to the sZr-BTB prepared by the invention, the material can be fixed on the surface of yarrowia lipolytica through a physical adsorption principle to form a layer of compact protective film, so that relatively macromolecular glycosidase is limited in cells and cannot be secreted, the excretion of small molecular aryl glycoside compounds is not influenced, the decomposition of a biosynthetic main product is avoided, and the yield of the yarrowia lipolytica is improved. Meanwhile, stable adsorption with yarrowia lipolytica can be maintained under a strong acidic condition, and the yield of aryl glycoside compounds produced by fermentation can be increased, so that the obstruction of non-mode microorganisms is solved, and low-cost industrialization of biosynthesis is promoted.

The invention discloses bacillus subtilis, a method for preparing yellow corn silage feed under a strong alkali condition and a product thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is that the survival rate of the existing bacillus subtilis is lower than 30% under the strong alkali condition of pH 9-11, and the existing bacillus subtilis cannot normally grow and propagate. The preparation of the yellow corn silage feed under a strong alkali condition needs additional pH adjustment, the process is complex and the cost is high; the crude protein content of the existing yellow corn silage feed is only 3-5%, and the requirements of livestock cannot be met. According to the technical scheme, the strong-alkali-resistant bacillus subtilis is provided, the preservation number is CGMCC No.36677, the survival rate of the strain is larger than or equal to 80% under the condition that the pH is 9-11, the straw degradation rate is larger than or equal to 25%, no waste water is generated when the strain is used for preparing the yellow silage feed, the cost is reduced compared with an existing technology, the protein content is increased by 50%-80%, palatability is good, and the method is suitable for large-scale straw resource utilization and feed protein production.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

Provided herein is an epigenetic modifying DNA targeting system, such as a CRISPR-Cas / guide RNA (gRNA) system, for transcriptional suppression of hepatitis B virus (HBV) genes to promote a cellular phenotype conducive to reduced HBV infection. In some embodiments, the epigenetic modifying DNA targeting system binds to or targets a target site of at least one gene or its regulatory element within the hepatitis B virus DNA sequence in a cell. In some aspects, the provided system relates to transcriptional suppression of one or more hepatitis B virus genes and / or their regulatory elements. In some aspects, methods and uses related to the provided compositions are also provided herein, for example, methods and uses in suppressing the replication and expression of hepatitis B virus associated with hepatitis B infection. TIFF2025527567000256.tif89170

The present invention includes modified and unmodified microbes, compositions comprising these microbes, and methods of using these microbes and / or compositions for enhancing plant health, plant growth plant yield and / or drought tolerance. This disclosure also provides non-naturally occurring plant varieties that are artificially infected with microbes descried herein, as well as seed, reproductive tissue, vegetative tissue, regenerative tissues, plant parts, or progeny thereof.

The present invention addresses the problem of providing: a method for preparing hepatic lineage cells in a large amount and with high purity; and a method for preparing liver system cells which can achieve high graft survival rate in a living body.　The present invention provides: a hepatoblast proliferation promoter and a culture medium, each of which contains a ROCK inhibitor, a TGFβ signaling pathway inhibitor, a Wnt signaling pathway activator, and a growth factor; and a proliferation / culture method using the hepatoblast proliferation promoter or the culture medium.

Provided are: a method for producing pancreatic endoderm cells, which comprises a step for culturing pancreatic endoderm cells in a culture medium containing a ROCK inhibitor and KGF and / or EGF; and pancreatic endoderm cells produced by the method.

The present invention shortens a period until an appropriate environmental parameter is obtained in a cell culture experiment.　According to the present invention, a control device sets a recipe for each cell sample, and controls a robot device to perform a culture experiment according to the recipe. A database device sequentially stores intermediate progress data for each sample during one culture experiment in which a culture result is determined. A recipe determination device analyzes tendencies of the culture on the basis of the intermediate progress data for each sample, and on the basis of the analysis result and difference as to time-series intermediate progress data of the sample, calculates posterior probability indicating a degree of probability leading to future success of the culture, with respect to values of a plurality of environmental parameters making up the recipe being set. The recipe determination device uses one or more environmental parameters having a relatively high posterior probability as a verification parameter, and modifies a value of the verification parameter for each sample to correct the recipe. The control device controls the robot device such that the experiment is continued according to the corrected recipe.