1190results about "Microorganisms" patented technology

The invention discloses a bacterial inhibitor for sea bass preservation, the bacterial inhibitor is bacteriocin generated by fermentation of lactobacillus plantarum L13 (the preservation number is CGMCC NO.27398), the bacteriocin comprises active polypeptide xylAB, the amino acid sequence of the xylAB is LLSSLATTGAIALGATAAKA, the preservation number of the bacterial inhibitor is CGMCC NO.27398, the preservation number of the bacterial inhibitor is CGMCC NO.27398, and the preservation number of the bacterial inhibitor is CGMCC NO.27398. The fresh-keeping agent prepared by the invention has a good fresh-keeping effect, can prolong the fresh-keeping period, and has huge potential as a natural food preservative in the food industry.

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

The invention relates to the technical field of aquaculture, and particularly discloses paracoccus pantotrophus P.p-O8 and application of the paracoccus pantotrophus P.p-O8, the paracoccus pantotrophus P.p-O8 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the paracoccus pantotrophus P.p-O8 is CGMCC NO.26522. The invention relates to a microbial inoculum prepared from paracoccus pantotrophus P.p-O8. The method has a huge application prospect in improving the quality of aquaculture water and repairing the polluted sediments in the cage culture area, improves the water environment quality and the survival conditions of aquaculture organisms, repairs the eutrophicated polluted ecological environment, and has high environmental protection value and economic benefit.

The invention discloses a deep-sea microorganism in-situ directional culture device, and relates to the technical field of deep-sea equipment, the deep-sea microorganism in-situ directional culture device comprises a microorganism culture cabin, a nutrient solution storage module, a bidirectional flow path assembly, an intelligent control module, a support assembly and the like; wherein the microorganism culture cabin consists of a flow channel, a flow choking block, a culture chamber, a movable small plate, a cabin cover and a sealing element, and the flow channel and the flow choking block form a structure based on a Tesla valve principle; through a bidirectional flow path assembly composed of a deep-sea high-precision metering pump, a deep-sea flow guide pump and the like, a nutrient substrate is injected in a reverse high-resistance mode to achieve efficient mixing, and seawater flows in a forward low-resistance mode to achieve environmental substance exchange; the deep-sea multi-channel switching valve is independently connected with the multiple sets of nutrient solution storage modules and the microorganism culture cabin, so that culture under various environmental conditions is realized; based on sensor data calculation and analysis, the starting and stopping time of the pump is intelligently controlled, the concentration of nutrient substrates in the microorganism culture cabin and the stability of environmental parameters are effectively guaranteed, and therefore in-situ culture deep-sea microorganism communities are controllable.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

The invention discloses bacillus subtilis, a method for preparing yellow corn silage feed under a strong alkali condition and a product thereof, and belongs to the technical field of microorganisms. The technical problem to be solved is that the survival rate of the existing bacillus subtilis is lower than 30% under the strong alkali condition of pH 9-11, and the existing bacillus subtilis cannot normally grow and propagate. The preparation of the yellow corn silage feed under a strong alkali condition needs additional pH adjustment, the process is complex and the cost is high; the crude protein content of the existing yellow corn silage feed is only 3-5%, and the requirements of livestock cannot be met. According to the technical scheme, the strong-alkali-resistant bacillus subtilis is provided, the preservation number is CGMCC No.36677, the survival rate of the strain is larger than or equal to 80% under the condition that the pH is 9-11, the straw degradation rate is larger than or equal to 25%, no waste water is generated when the strain is used for preparing the yellow silage feed, the cost is reduced compared with an existing technology, the protein content is increased by 50%-80%, palatability is good, and the method is suitable for large-scale straw resource utilization and feed protein production.

The invention discloses a bifidobacterium animalis subsp. Lactis BA-C53 and an application of the bifidobacterium animalis subsp. Lactis BA-C53 in products for improving bone health. The bifidobacterium animalis subsp. Lactis BA-C53 is classified and named as bifidobacterium animalis subsp. Lactis Bifidobacterium animalis subsp. Lactis BA-C53, the preservation number is CCTCC NO: M 2024460, the preservation unit is China Center for Type Culture Collection, and the preservation time is March 11, 2024. The bifidobacterium animalis subsp. Lactis BA-C53 can effectively improve the steady state of intestinal flora of a mouse with osteoporosis induced by estrogen deficiency, so that the relative abundance of phylum firmicalis and phylum actinomycetes is increased, the relative abundance of phylum bacteroides is reduced, and then short-chain fatty acid is generated to activate intestinal immunity and maintain the integrity of an intestinal barrier, so that the osteoporosis caused by estrogen deficiency is inhibited. Meanwhile, osteoclast differentiation can be inhibited by regulating NFATC1, RANKL and OPG pathways, then osteoporosis is relieved, and the compound can be widely applied to preparation of food and drugs.

The invention relates to the technical field of food, in particular to geobacillus stearothermophilus cKM-Geo, application of the geobacillus stearothermophilus cKM-Geo, a shrimp shell oligosaccharide peptide compound and a preparation method of the shrimp shell oligosaccharide peptide compound. The invention provides geobacillus stearothermophilus cKM-Geo, the strain can grow in an oxygen-free environment, ventilation is not needed in the process, and proteolytic enzyme, cellulase, lipase and chitin deacetylase can be highly secreted and expressed; the invention further provides a preparation method for preparing the shrimp shell oligosaccharide peptide by utilizing geobacillus stearothermophilus cKM-Geo fermentation coupling steam explosion treatment, chitin and protein in the shrimp shell are converted into water-soluble oligopeptide and oligosaccharides, the water-soluble oligopeptide and oligosaccharides are separated from calcium carbonate in the shrimp shell, acid and alkali do not need to be used, in the process, waste water is little, pollution is little, and the method is suitable for industrial production. The resource utilization rate is high.

The present invention provides a method for producing a cartilage-like tissue from pluripotent stem cells, the method comprising a step for culturing pluripotent stem cells in a cartilage differentiation culture medium and a step for increasing the ascorbic acid concentration of the cartilage differentiation culture medium. The present invention also provides a method for regulating the hardness of induced cartilage-like tissue differentiated from pluripotent stem cells, the method comprising a step for culturing pluripotent stem cells in a cartilage differentiation culture medium and a step for increasing the ascorbic acid concentration of the cartilage differentiation culture medium.

The invention discloses lactobacillus plantarum N4 and application thereof. The lactobacillus plantarum N4 is characterized in that milk white circular bacterial colonies with the diameter of 1-2 mm are formed after the lactobacillus plantarum N4 is cultured on an MRS solid culture medium at 37 DEG C for 48 h, and the milk white circular bacterial colonies are smooth and wet in surface and neat in edge. The strain has excellent salt resistance, acid production capacity and flavor substance generation capacity, can remarkably shorten the fermentation period, reduce the nitrite content in the fermentation process and improve the flavor and quality of products when being applied to the pure-variety intensified fermentation of the pickled Chinese cabbages, solves the problems of low fermentation efficiency, unstable product quality and the like of traditional fermented food, and has a wide application prospect. The method has high industrial application value.

The invention discloses a bacillus thuringiensis CMS-BT17, which is classified and named as bacillus thuringiensis, is preserved in the China General Microbiological Culture Collection Center (CGMCC), has the preservation number of CGMCC 35313, and is preserved on July 21, 2025. The bacillus thuringiensis strain CMS-BT17 directly separated and screened from a zizania aquatica soil sample in Haikou City of Hainan carries four insecticidal protein coding genes of Cry8Ca, Cry8Da, Spp1Aa and Vpb4Ca, not only is high in insecticidal toxicity and good in insecticidal effect, but also has a certain effect of inhibiting tomato fungal diseases, can be widely applied to prevention and control of agricultural diseases and pests, and has a wide application prospect. The sustainable development of green agricultural production is promoted.

The invention relates to the technical field of microorganisms, and particularly discloses a lactobacillus paracasei KQNIU, a microbial preparation and application of the lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is preserved in the China General Microbiological Culture Collection Center on August 25, 2025, the preservation number is CGMCC No.35739, and the Lactobacillus paracasei KQNIU is classified and named as Lactobacillus paracasei, and the Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The Lactobacillus paracasei KQNIU is named as Lactobacillus paracasei KQNIU. The lactobacillus paracasei KQNIU provided by the invention has good gastrointestinal tract tolerance, has an inhibition effect on pathogenic bacteria such as escherichia coli, salmonella and the like, can be prepared into a microbial preparation or a feed additive for preventing and treating calf diarrhea, replaces antibiotics and realizes green and healthy breeding.

The invention relates to the technical field of agricultural microorganisms, and discloses stress-tolerant bacillus velezensis L-29 and application thereof. The bacillus velezensis L-29 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35587. The bacillus velezensis L-29 is named as bacillus velezensis L-29. Experiments prove that the bacillus velezensis L-29 can adapt to various extreme environments, can survive and proliferate under the extreme environments of drought conditions simulated by 0-25% PEG 6000 under the conditions that the pH is 2-12 and the salt concentration is 1.5%, and has various functions of soil improvement (organic matter decomposition and phosphorus activation), plant growth promotion, broad-spectrum disease control and the like. In the secondary fermentation stage of compost, humus generation can be remarkably promoted, compost maturation is accelerated, the good colonization ability is achieved, and after an obtained compost product is applied to normal soil, the nutrient effectiveness can be improved, crop growth is promoted, and the disease resistance is enhanced.

The invention relates to paenibacillus strongly ZKSW001 as well as a fungicide and application thereof, and belongs to the technical field of microorganisms. The paenibacillus pumilus ZKSW001 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.36076, the preservation date is September 25, 2025, and the address of the preservation institution is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The invention also provides application of the paenibacillus strongly ZKSW001 in inhibition of harmful bacteria in soil, prevention and treatment of root-knot nematode and promotion of plant growth. The paenibacillus strongly ZKSW001 disclosed by the invention not only can promote the growth of crops and realize weight loss and efficiency improvement, but also can control the occurrence of soil-borne fungal and bacterial diseases, and can inhibit the growth of root-knot nematode. Chemical pesticide application can be reduced, pesticide residues are reduced, and sustainable development of agricultural economy is promoted.

The present invention relates to a probiotic composition comprising at least one strain selected from the group consisting of Lactobacillus paracasei LPC1082, Lactobacillus paracasei LPC1101 and Lactobacillus paracasei LPC111, optionally in combination with at least one strain selected from the group consisting of Phytobacterium plantarum PBS067 and Lactobacillus rhamnosus LRH020, and at least one excipient and / or a physiologically acceptable carrier, the composition is used for preventing and / or treating pathological conditions and / or diseases of the oral cavity.

The invention relates to genetic engineering combined lactobacillus salivarius and application of the genetic engineering combined lactobacillus salivarius. The strain is classified and named as combined lactobacillus salivarius CTMT2, and the preservation number of the strain is CCTCC (China Center for Type Culture Collection) M 2025547. After an NIAAA model is injected into the stomach of an alcoholic liver mouse, the acetaldehyde level of the liver of the mouse is obviously reduced, and the alcoholic fatty liver symptom of the mouse is obviously relieved; after long-term intragastric administration of a high-glucose and high-fat modeling mouse, the acetaldehyde level in excrement of the mouse can be obviously reduced, the progress of liver fibrosis is improved, and good application prospects are achieved.

The present disclosure relates to yeast fermentation broths of salidroside for animal husbandry applications, animal feed formulations incorporating the same, and methods of forming and administrating the same.

The invention discloses a high-performance antagonistic bacterium RQH1 and application of the high-performance antagonistic bacterium RQH1 in prevention and control of amorphophallus konjac soft rot. The high-performance antagonistic bacterium RQH1 is preserved in China Center for Type Culture Collection, the preservation number is CCTCC NO. M 2023719, the preservation address is Wuhan University, Wuhan, China, and the preservation time is May 10, 2023. According to the invention, a high-performance antagonistic bacterium RQH1 is obtained by directional screening in a healthy konjak plant body, and the strain not only shows broad-spectrum and high-efficiency bacteriostatic activity on various konjak soft rot pathogenic bacteria, but also obviously inhibits the pathogenic bacteria from growing and infecting; meanwhile, various growth promoting functions such as phosphorus dissolving, potassium dissolving and nitrogen fixing can be achieved, the soil nutrient condition can be improved, and konjak plants can be promoted to grow robustly. In addition, the composition and antibacterial mechanism of volatile metabolites of the RQH1 strain are systematically analyzed. Therefore, a high-value strain resource which is multifunctional, easy to culture and high in adaptability is provided for green prevention and control of the soft rot of the konjak, and various defects in the prior art are effectively overcome.

The invention provides a mulberry phellinus linteus strain SH08, mulberry phellinus linteus polysaccharide as well as a preparation method and application of the mulberry phellinus linteus polysaccharide, and belongs to the technical field of biological medicines and functional foods. The mulberry phellinus igniarius strain SH08 provided by the invention is preserved in the China Center for Type Culture Collection on October 11, 2025, the preservation address is No. 299, eight road, Wuchang District, Wuhan City, Hubei Province, the strain preservation number is CCTCC NO: M20252203, and the mulberry phellinus igniarius strain SH08 is named as SanghuangAKSQSH01. The invention also provides a method for preparing high-purity mulberry phellinus linteus polysaccharide (SHP-PS) from sporocarp of the strain, and structural characterization of the polysaccharide. Experiments prove that the SHP-PS can effectively improve non-alcoholic fatty liver disease (NAFLD) induced by high fat diet, and the mechanism of the SHP-PS is closely related to blood fat regulation, liver injury and inflammation reduction, intestinal barrier repair, intestinal flora structure regulation and short-chain fatty acid generation promotion. The invention provides high-quality raw materials and a clear technical scheme for developing novel medicines or functional foods for preventing and treating NAFLD, and has important application value.

The invention relates to the technical field of microorganisms, in particular to Cryptoporus volvatus zaasMS-9 which is named as Cryptoporus volvatus zaasMS-9. The Cryptoporus volvatus zaasMS-9 is characterized in that cultivation is carried out in a culture medium containing mulberry sawdust, the fruiting time is 16-20 days, and the fruiting body harvesting time is 7-10 days; the biological material preservation information of the strain is as follows: the preservation number, the preservation unit and the preservation time. The invention further provides an indoor or under-forest cultivation method of cryptoporus sinensis. The method sequentially comprises the following steps of mother strain preparation, cultivated species preparation, ganoderma lucidum fruiting fungus bag preparation, inoculation, spawn running culture, fruiting management and harvesting.

This adsorbent for endotoxin in a culture medium contains at least one selected from the group consisting of anionic clay, a solid solution of anionic clay, and magnesium oxide, and can effectively adsorb ET in the culture medium. Representative examples of the anionic clay include hydrotalcite. The endotoxin adsorbent may further contain an adsorbent comprising a base material and a cationic group including a nitrogen atom. The adsorbent comprising a base material and a cationic group including a nitrogen atom is preferably a cellulose having an amino group.

The invention discloses debaryomyces hansenii with high yield of umami peptide and application of the debaryomyces hansenii in improving the umami of low-salt fermented sausages, the debaryomyces hansenii is separated from the anglerkang sausages, is preserved in the China General Microbiological Culture Collection Center on September 30, 2025, and has the preservation number of CGMCC (China General Microbiological Culture Collection Center), and the preservation number of the debaryomyces hansenii HYYAKS044 is CGMCC NO. The biological preservation number is CGMCC (China General Microbiological Culture Collection Center) The content of peptides below 3kDa of the sausage inoculated with the debaryomyces hansenii disclosed by the invention is obviously more than that of a non-inoculated group, and six umami peptides, namely DDHFLFDKP, DPTPSDGKL, FPPDVGGN, ISEQFTAMFR, PETPPP and VDNPGHPFI, can be screened from the peptides below 3kDa, and the six umami peptides are respectively DDHFLFDKP, DPTPSDGKL, FPPDVGGN, ISEQFTAMFR, PETPPP and VDNPGHPFI.

The application belongs to the technical field of microorganisms and particularly relates to a kind of Aspergillus ustus and application thereof.The Aspergillus ustus is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.40820.The Aspergillus ustus can be separated to obtain sesquiterpenes shown in formula I and formula II, and the sesquiterpenes have good bacteriostatic activity on Physarum polycephalum, and the bacteriostatic rates are 79.45% and 76.71% respectively at a final concentration of 100 μg / mL, which is equivalent to the control agent of bercyn in inhibiting mycelial growth.

Provided is a detection technique excellent in cell drying resistance, cell retention, and detection of a chemical substance such as an odorant. More specifically, provided are: a laminate containing

The application provides a method for treating and recycling iron-containing waste sulfuric acid in titanium dioxide production, which is based on screening of a strain capable of treating iron-containing waste water in a low pH environment, and establishes a method for treating iron-containing waste sulfuric acid, which can effectively realize resource recycling. The waste water treatment bacteria used are Bacillus QD-QJ-062 strain, and the preservation number is CGMCC No.33725. The QD-QJ-062 strain of Bacillus screened by the application can effectively treat the iron-containing waste water in a low pH environment, promote the generation of ferrous oxalate precipitation, and generate ferrous oxalate by-products. The generated ferrous oxalate can be converted into ferrous sulfate by sulfuric acid, and oxalic acid can be recycled, thereby reducing the operation cost. The sulfuric acid solution after iron removal can be neutralized by limestone to generate high-purity gypsum, thereby realizing resource recycling and treatment.

The invention discloses a method for extracting astaxanthin from phaffia rhodozyma by combining bacillus fermentation with ionic liquid. The method comprises the following steps: carrying out co-culture on bacillus velezensis Y12 and phaffia rhodozyma, so as to obtain a fermentation solution; mixing the fermentation liquor with ionic liquid in equal volume, stirring, centrifugally separating out the ionic liquid containing astaxanthin, and finally synthesizing the novel astaxanthin emulsion with water and sunflower seed oil. The preservation number of the bacillus velezensis Y12 is CGMCC (China General Microbiological Culture Collection Center) No.27125, and the bacillus velezensis Y12 can be used for preparing the bacillus velezensis. According to the method, the extraction rate of the phaffia rhodozyma astaxanthin is remarkably increased, meanwhile, the antioxidant activity and stability of the astaxanthin are effectively improved, and the method has important industrial application value.

The invention belongs to the technical field of microorganisms, and relates to a bifidobacterium breve strain capable of relieving constipation, the strain is bifidobacterium breve Bb-PG-14, the classification name of the strain is Bifidobacterium breve, the preservation number is CGMCC No.31861, the preservation time is September 4, 2024, the preservation unit is China General Microbiological Culture Collection Center, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, the preservation number is CGMCC No.31861, and the preservation number is CGMCC No.31861. The address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain is separated and purified from excrement of healthy babies, has relatively strong acid resistance, alkali resistance, cholate resistance and artificial simulated gastrointestinal fluid tolerance, and has the characteristics of probiotics.

The present invention relates to a Bacillus thuringiensis JNU01 (accession number: KCTC 14427BP) strain having a plastic degradation activity. The Bacillus thuringiensis JNU01 strain according to the present invention is able to degrade plastic when cultured in a medium comprising the plastic, and thus may be used in a pretreatment process for plastic recycling.