2447results about "Microorganisms" patented technology

The invention relates to the technical field of agricultural biology, and discloses an auxiliary cultivation method for promoting sugarcane biological nitrogen fixation, which comprises the following steps: (1) breeding a sugarcane variety with high nitrogen-fixing bacteria affinity, and preparing an environmental stress resistant nitrogen-fixing bacteria agent; (2) carrying out acidification improvement and carbon source pre-burying on the planting soil; (3) inoculating the nitrogen-fixing bacterial agent to the sugarcane seed stem or rhizosphere area; (4) maintaining the metabolic activity of nitrogen-fixing bacteria by dynamically regulating and controlling rhizosphere carbon source supply and oxygen partial pressure; (5) adjusting a fungicide supplementary application strategy in real time based on the environmental data; and (6) solidifying the symbiotic character through intergenerational epigenetic treatment. Through host-microorganism coevolution and intelligent dynamic regulation and control, the nitrogen fixation efficiency and environmental adaptability of the sugarcane-nitrogen-fixing bacteria symbiotic system are remarkably improved; and epigenetic and flora iteration technologies are combined, so that stable transmission of cross-generation characters is realized, and a low-consumption and high-benefit ecological solution is provided for sustainable agriculture.

To provide a technology that balances increased food production and control of environmental pollution that can cope with the increasing population.SOLUTION: After the agrochemicals used on the crops fulfilled their function, the ascomycetes white wood-rotting fungus having the characteristics of mycorrhizal fungus is used to decompose the agrochemicals remaining on the crops and soil.SELECTED DRAWING: Figure 4

A technique related to codon optimization is provided. In a method for optimizing nucleic acid sequences expressing proteins in a host, protein subsequence-nucleic acid subsequence pairs are obtained from collected highly expressed protein sequences and their encoding nucleic acid sequences to form a training set. And training a neural machine translation model by using the training set. The neural machine translation model realizes translation from an amino acid sequence to a codon sequence. A protein sequence needing codon optimization is cut into protein subsequences. And translating a protein subsequence from an amino acid sequence to a codon sequence by using the trained neural machine translation model. The translated subsequences are superimposed to merge into a full-length codon sequence. In the process of superimposing to synthesize the codon sequence, according to the frequency or number of synonymous codons corresponding to each amino acid position of the protein sequence, the synonymous codon with the highest occurrence frequency or number is selected as the optimal codon at the position.

Provided are a strain of Lactobacillus plantarum, i.e., Lactobacillus plantarum PDG8, and compositions thereof. Also provided are methods of treating cardiovascular diseases, such as atherosclerosis, using Lactobacillus plantarum PDG8 or a composition thereof.

The invention relates to the technical field of microbial culture, and provides a culture device for agricultural microbial agent production and a use method, the culture device comprises a culture box, and the top of the culture box is hermetically and detachably provided with a box cover. According to the device, the air pump is controlled by the controller to be started, so that the air pressure in the closed space is increased, the air passes through the non-woven fabric and the polymer microporous membrane, more tiny bubbles can be generated under the action of the polymer microporous membrane, a nutrient solution flows and is mixed, and the stirring effect on the nutrient solution is achieved; a nutrient solution in the incubator flows into the culture dish through the liquid discharge pipe, so that the stirring of the nutrient solution and the injection of the nutrient solution into the culture dish to be mixed with a microbial inoculum can be completed without using more structural components, the operation is simple, and the condition of pollution caused by improper cleaning is avoided; therefore, the subsequent microbial agent culture effect is improved.

The subject invention provides compositions and methods for remediating and mitigating iron pollution. In preferred embodiments, a composition comprising an iron-capturing ingredient is contacted with an environment suffering from iron pollution in order to reduce the amount of iron in the environment.

A cell culture system includes: a plurality of cell culture channels each of which includes a fluidic device in which cells are cultured, a pump which makes a liquid flow into the fluidic device, a detection unit which detects a state of the cells that are cultured, and a controller which controls the pump and the detection unit; a measurement unit that measures culture environment of the cells which are cultured in the plurality of cell culture channels; and an information processing apparatus that adjusts the culture environment such that an absolute value of a difference between evaluation values for the states of the cells that are cultured in the plurality of cell culture channels is a threshold value or less, on the basis of the state of the cells detected by the detection unit and the culture environment of the cells measured by the measurement unit.

The invention discloses a bacterial inhibitor for sea bass preservation, the bacterial inhibitor is bacteriocin generated by fermentation of lactobacillus plantarum L13 (the preservation number is CGMCC NO.27398), the bacteriocin comprises active polypeptide xylAB, the amino acid sequence of the xylAB is LLSSLATTGAIALGATAAKA, the preservation number of the bacterial inhibitor is CGMCC NO.27398, the preservation number of the bacterial inhibitor is CGMCC NO.27398, and the preservation number of the bacterial inhibitor is CGMCC NO.27398. The fresh-keeping agent prepared by the invention has a good fresh-keeping effect, can prolong the fresh-keeping period, and has huge potential as a natural food preservative in the food industry.

The present invention relates to a recombinant yeast cell that produces UDP-D-glucuronic acid and / or UDP-N-acetyl-D-glucosamine compared to a wild type cell of the recombinant yeast cell; and optionally, the yield of 3 '-phosphate-adenylate sulfuric acid is increased. The invention also relates to a method for producing heparan sulfate, which comprises a step of culturing the recombinant yeast cell, further relates to an application of the non-invasive recombinant yeast cell for producing heparan sulfate, and finally relates to heparan sulfate obtained by applying the method.

The invention provides pantoea agglomerans and application thereof, and belongs to the technical field of microorganisms, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.36122; the strain has very high selenium tolerance and transformation capacity, and can transform inorganic selenium (such as Se (IV)) with relatively high toxicity into organic selenium (such as selenomethionine and selenocystine) with low toxicity and zero-valent nano-selenium (Se0) in a culture medium containing sodium selenite. The pantoea agglomerans obtained by the invention has the characteristics of selenium-addictive microorganisms, and has potential application value in biotransformation of selenium, microbial synthesis of nano-selenium, and growth promotion and selenium enrichment of crops. The pantoea agglomerans can promote plant growth, a liquid fermentation process developed by the strain is utilized, the highest conversion rate of organic selenium can reach 92.5%, and the synthesis efficiency of nano-selenium reaches 88.3%. And development of organic selenium agricultural products is well facilitated.

Provided herein are binding molecules comprising a human neonatal Fc receptor (FcRn) binding molecule and a moiety that does not specifically bind to a human antigen. Polynucleotides, vectors, host cells, and methods of production are also provided herein. Methods of treating an antibody-mediated disorder with an FcRn binding molecule or an FcRn / antigen-binding molecule are further provided.

The invention relates to a method for evaluating and debugging a sterile pipeline for food and beverage processing, which comprises the following steps of: introducing standardized sterile gas into a closed pipeline system to establish a micro-positive pressure environment; detecting a pressure change curve of each node of the system in real time by using a micro-differential pressure sensing module; calculating the position and the size of a trace leakage point; recording the motion trail of the particles in the pipeline by using a dynamic particle imaging system PIV; flow dead angles are identified through trajectory analysis, and the potential pollution accumulation area of the retention area marks the pollution accumulation probability of each section of pipeline in a quantitative mode; preparing simulation liquid, and adding biomarker particles to enable the viscosity and the fluidity of the target beverage to be close to those of the target beverage; according to the identified potential pollution accumulation area, local directional pouring is carried out; full-process automatic data acquisition, model judgment and debugging instruction issuing can be realized, manual intervention is greatly reduced, the system stability is improved, and the system is suitable for various food and beverage production scenes.

Stem cells can be cultured by suspension culture while improving functionalities of the stem cells by treating the stem cells with a water-soluble polymer (for example, culturing in the presence of the water-soluble polymer). Such a method is a more versatile culture method with low cost.

The invention relates to the technical field of aquaculture, and particularly discloses paracoccus pantotrophus P.p-O8 and application of the paracoccus pantotrophus P.p-O8, the paracoccus pantotrophus P.p-O8 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the paracoccus pantotrophus P.p-O8 is CGMCC NO.26522. The invention relates to a microbial inoculum prepared from paracoccus pantotrophus P.p-O8. The method has a huge application prospect in improving the quality of aquaculture water and repairing the polluted sediments in the cage culture area, improves the water environment quality and the survival conditions of aquaculture organisms, repairs the eutrophicated polluted ecological environment, and has high environmental protection value and economic benefit.

The present invention relates to: a transformed strain expressing and secreting Akkermansia muciniphila TARS (AmTARS); and a vector for producing same. More specifically, the present invention relates to an Escherichia coli Nissle 1917 (EcN) strain expressing AmTARS, a composition comprising same, a novel vector for producing same, and an EcN transformation method using same.

The invention discloses a deep-sea microorganism in-situ directional culture device, and relates to the technical field of deep-sea equipment, the deep-sea microorganism in-situ directional culture device comprises a microorganism culture cabin, a nutrient solution storage module, a bidirectional flow path assembly, an intelligent control module, a support assembly and the like; wherein the microorganism culture cabin consists of a flow channel, a flow choking block, a culture chamber, a movable small plate, a cabin cover and a sealing element, and the flow channel and the flow choking block form a structure based on a Tesla valve principle; through a bidirectional flow path assembly composed of a deep-sea high-precision metering pump, a deep-sea flow guide pump and the like, a nutrient substrate is injected in a reverse high-resistance mode to achieve efficient mixing, and seawater flows in a forward low-resistance mode to achieve environmental substance exchange; the deep-sea multi-channel switching valve is independently connected with the multiple sets of nutrient solution storage modules and the microorganism culture cabin, so that culture under various environmental conditions is realized; based on sensor data calculation and analysis, the starting and stopping time of the pump is intelligently controlled, the concentration of nutrient substrates in the microorganism culture cabin and the stability of environmental parameters are effectively guaranteed, and therefore in-situ culture deep-sea microorganism communities are controllable.

The invention discloses a process for repairing damaged soil ecology by lincomycin waste liquid fertilizer, and relates to the technical field of soil remediation, which comprises the following specific steps: step 1, screening and culturing microbial decomposition flora, the invention has the following advantages: through the chelation of the polyaspartic acid derivative on metal ions and the promotion effect on nutrient absorption, the release and transmission of nutrients are precisely regulated and controlled, so that crops can absorb and utilize the nutrients more efficiently; the nano composite material and the specific biological stimulant containing various beneficial microbial strains and natural plant extracts are jointly fused into the liquid fertilizer, the microbial strains in the specific biological stimulant activate the soil biological environment, the natural plant extracts assist plant growth to resist diseases, and the nano composite material and the natural plant extracts have a synergistic effect, so that the liquid fertilizer has a good application prospect. Physicochemical properties and biological activity of soil are optimized, excellent soil micro-ecology is created for plant growth, healthy and strong growth of plants is promoted, and restoration of damaged soil is enhanced.

To provide an inhibitor for suppressing expression of an antibacterial peptide in brown planthopper and an application thereof.SOLUTION: A dsNlPCE4 inhibitor designed based on the NlPCE4 gene of the brown planthopper is obtained. The inhibitor, when applied to brown planthoppers inoculated on their body surface with Metarhizium anisopliae, significantly increases the mortality of the brown planthopper. The present invention combines two technical strategies for pest control, biocontrol fungi and RNA interference (RNAi), to provide a new target for brown planthopper control technology based on cooperative mediation of RNAi.SELECTED DRAWING: Figure 5

The present disclosure relates to an application of Antarctic red pigment in a preparation of drugs for treatment and prevention of hyperuricemia, which belongs to the field of biomedicine. The present disclosure studies a fermentation product of existing fungi Geomycos sp. wnf-18C or Geomycos sp. wnf-15A, and finds that the Antarctic red pigment produced by the fermentation product has good therapeutic effects on hyperuricemia induced by high purines. Thus, this pigment has application value in the preparation of drugs for hyperuricemia induced by high purine.

The invention discloses an identification method based on microorganism culture and detection and an identification system based on microorganism culture and detection suitable for the method. The method comprises the steps of bottling, culture and detection, conveying to a plate transfer area, upwards pricking a bottle opening for deflation, taking a plate to be used, scribing the plate, culturing in an incubator, detection and recycling the plate. Aerosol released by a culture bottle is pumped away in the bottle opening upward hole pricking and deflating step, a blood sample or other body fluid in a plate is prevented from being polluted by the aerosol, the identification system comprises a case, a blood sampling device and an air pumping device, the blood sampling device comprises a first culture bottle clamping jaw and a seed transferring device clamping jaw, and when the seed transferring device clamping jaw is located above the first culture bottle clamping jaw, the air pumping device is used for pumping air into the first culture bottle clamping jaw. The needle head of the seed transferring device is inserted into the culture bottle, and when the culture bottle releases gas, the gas is pumped away in time by the gas pumping device, so that a blood sample or other body fluid in the plate is prevented from being polluted, and the treatment and detection accuracy of the blood sample or other body fluid is improved.

The invention belongs to the technical field of microorganisms, and particularly relates to bacillus cereus as well as a culture and application thereof. The preservation number of the bacillus cereus AK-LY1 is CCTCC (China Center For Type Culture Collection) NO: M 2025756. The bacillus cereus AK-LY1 disclosed by the invention is excellent in trypsin resistance, good in cholate resistance, acid resistance and high temperature resistance, good in inhibition performance on antibiotics such as strong penicillin and chloramphenicol, and relatively good in adhesion capacity in intestinal tracts, and provides a relatively good microecological preparation reference basis for expanding a bacillus cereus resource library and applying the bacillus cereus.

The present invention relates to a method and an apparatus for converting carbon monoxide and / or carbon dioxide and hydrogen in a gas to methane and water. The gas containing carbon monoxide and / or carbon dioxide is preferably biogas, flue gas or synthesis gas. The gas containing hydrogen is preferably a gas obtained from the electrolysis of water. The method and apparatus include efficient washing of the packaging material without removing it from the reactor, thereby maintaining and optimizing the conversion efficiency. In particular, the present invention relates to increasing the amount of green energy obtained from the anaerobic digestion of organic material by reducing the carbon dioxide content and increasing the methane content in the gas.

The invention discloses a phage inhibitor and application thereof. The phage inhibitor is prepared from the following components in parts by weight: 30 to 60 parts of carboxymethylated hippophae rhamnoides polysaccharide, 1.8 to 2.2 parts of citric acid, 2.3 to 2.7 parts of sodium citrate, 0.8 to 1.2 parts of D-sodium gluconate, 4 to 6 parts of dihydroxyethyl glycine, 1.8 to 2.2 parts of sodium hexametaphosphate and 0.8 to 1.2 parts of oxalic acid, and the amount of sodium hydroxide is added according to the pH value during solution preparation. The product is designed into a mixed prefabricated product; or liquid with various concentrations can be prepared and diluted according to needs during use. When the product is applied to a prokaryotic expression system, the pollution of phage to cells can be effectively inhibited, the efficiency and quality of scientific research and production are remarkably improved, and a more leisurely fermentation environment is provided for scientific research personnel. Meanwhile, the carboxymethylated sea-buckthorn polysaccharide, the citric acid, the sodium citrate and the oxalic acid contained in the phage inhibitor have a synergistic effect, so that the inhibition effect of the phage inhibitor on the phage can be remarkably improved.

The invention relates to the field of microorganism culture devices, and discloses a microorganism expanding culture device and an expanding culture method thereof. The microorganism expanding culture device comprises a cabinet body, wherein an expanding culture reaction mechanism, a supply mechanism and a control mechanism are arranged in the cabinet body; the propagation reaction mechanism comprises a propagation reaction cylinder, a cylinder cover plate, an electric device and a stirring assembly; the propagation reaction cylinder is fixedly connected with the cabinet body; the cylinder cover plate is connected with the electric device; the power output end of the electric device is connected with the stirring assembly through the barrel cover plate; the stirring assembly is arranged in the propagation reaction cylinder; the supply mechanism is connected with the barrel cover plate and comprises a hot water supply assembly, and the hot water supply assembly is connected with the barrel cover plate. The temperature suitable for efficient propagation of strains can be achieved when the temperature is low in winter, the intelligent degree of the propagation process is improved, accurate monitoring of the propagation process and device integration of subsequent equipment cleaning are achieved, and efficient propagation of microorganisms is achieved on the whole.

The invention relates to the field of drilling in the petroleum industry, and discloses a composition for a microbial stabilizer, the microbial stabilizer and application thereof, and a water-based drilling fluid and application thereof. The composition contains a thermophilic strain, a nutrient solution for the thermophilic strain, urea and a calcium source, the preservation number of the thermophilic strain is 1K04190; the content mass ratio of the thermophilic strain to the nutrient solution for the thermophilic strain to the urea to the calcium source is 1: (2-10): (1-10): (1-4); the OD600 (optical density 600) of the thermophilic strain is 0.4 to 0.6. The microbial stabilizer obtained by adopting the composition for the microbial stabilizer can effectively generate CaCO3 precipitates with high mechanical strength, can play a role in adjusting the rheological property of drilling fluid after being applied to the drilling fluid, can reduce the leakage of the drilling fluid in the drilling work, plays a role in stabilizing a well wall, and has the advantages that the drilling fluid is prevented from being damaged, and the service life of the drilling fluid is prolonged. Meanwhile, the mechanical strength of the rock can be obviously improved.

To provide a composition containing solubilized polyhydroxyalkanoate by which a membrane having excellent cosmetic characteristics can be obtained.SOLUTION: A composition contains a) at least two kinds of different repeating polymer units selected from the following units (A) and (B): -[-O-CH(R1)-CH2-C(O)-]-unit (A) and -[-O-CH(R2)-CH2-C(O)-]-unit (B) and an optical isomer or a geometric isomer thereof, and a solvate thereof such as a hydrate; and b) a fatty medium including one or more kinds of fatty substances which are liquid preferably at 25°C and the atmospheric pressure, where it is to be understood that (A) is different from (B).SELECTED DRAWING: Figure 2

The present invention provides a long-period high-pressure enrichment culture and online monitoring apparatus and method for deep-sea microorganisms. The apparatus can simulate extreme deep-sea environments such as high pressure, low temperature, and oligotrophic conditions. Through pre-culture and graded serial culture, stable long-period growth and reproduction of the deep-sea microorganisms are ensured. An online system is used for monitoring the abundance, activity, and culture conditions of the microorganisms in real time to determine an appropriate timing for strain subculturing, and timely interactive subculturing and culture are performed, thereby achieving stable supply of high-abundance, high-activity microorganism enrichment culture solution. The present invention achieves precise pressure-retaining abundance counting of the microorganisms under in-situ pressure conditions and accurate control of a microbial growth cycle during enrichment culture, effectively improving timeliness and culture efficiency of the microorganism enrichment culture solution, and enabling long-period acquisition of high-abundance, high-activity microbial strain liquid.

Embodiments of the invention relate generally to processes for the production and processing of polyhydroxyalkanoates (PHA) from carbon sources. In several embodiments, PHAs are produced at high efficiencies from carbon-containing gases through the utilization of a regenerative polymerization system.

To provide a novel method for oil-water separation of an oil-in-water suspension and an oil-water separation device.SOLUTION: A method for oil-water separation is provided, comprising: contacting an oil-in-water suspension with water, which may contain microbubbles, such that a suspension-rich phase is positioned above and a water-rich phase is positioned below; supplying new microbubbles to the water-rich phase so as to prevent uniform mixing between the suspension-rich phase and the water-rich phase; and floating microbubbles having adsorbed micelles in the suspension. Also provided is an oil-water separation device comprising: a separation tank that houses an oil-in-water suspension and water, which may contain microbubbles, such that a suspension-rich phase is positioned above and a water-rich phase is positioned below; and a microbubble generator that generates microbubbles to be supplied into the separation tank, wherein the microbubble generator is disposed such that the generated microbubbles are supplied to the water-rich phase, and the interior of the separation tank is not stirred during microbubble generation in a manner of preventing uniformity inside the tank.SELECTED DRAWING: Figure 1