132 results about "Complex protein" patented technology

The invention discloses oyster-soybean compound protein gel constructed based on low-temperature synergy as well as a preparation method and application of the oyster-soybean compound protein gel, and belongs to the technical field of functional foods. The composite gel is formed by oyster protein and soybean protein isolate through low-temperature stabilization, medium-temperature construction and thermal induction treatment. The preparation method comprises the following steps: preparing an oyster protein suspension; preparing a soybean protein isolate suspension; the oyster protein suspension and the soybean protein isolate suspension are mixed, stirred and left to stand, a gel structure is preliminarily constructed and then subjected to thermal induction curing treatment, and after the thermal induction curing treatment is finished, the oyster-soybean compound protein gel is formed through storage at low temperature and can be used in the field of food processing. The oyster protein and the soybean protein isolate are combined, so that the composite gel has excellent gel strength, smooth texture and long-acting storage stability, no organic chemical reagent or food additive is used in the preparation process, the formula safety is ensured, the process is simple and convenient, and the composite gel shows huge application value and industrialization prospect.

The invention relates to the technical field of agricultural fertilizers, in particular to an enzymolysis fish protein type amino acid-containing water-soluble fertilizer and a preparation method thereof, the fertilizer is prepared from 50-70% of enzymolysis fish protein stock solution and other components; the stock solution is prepared by pulping aquatic product processing leftovers, adjusting the pH value to 6.5-7.5, adding specific compound protease for enzymolysis, and performing enzyme deactivation and centrifugation; the macroelement liquid contains nitrate nitrogen and ammonium nitrogen (2: 1-4: 1), and the microelement chelating agent is prepared by reacting a stock solution small molecule peptide with a specific salt. The fish protein enzymolysis stock solution is prepared from aquatic product processing leftovers, high-proportion small molecule peptide and amino acid are obtained through specific enzymolysis and other processes, and by matching with high-content macroelements in a specific nitrogen form, nutrition can be rapidly supplemented, and crop photosynthesis and dry matter accumulation can be improved; and moreover, all the components cooperate to regulate the growth of the crops and improve the stress resistance, and the surfactant also improves the utilization rate of the fertilizer and reduces waste.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention discloses an easily absorbable organic calcium powder containing mineral elements and a preparation process thereof, and the raw materials comprise, by weight, 50-70 parts of an organic calcium source, 20-30 parts of a plant protein source, 5-10 parts of a polybasic amino acid chelate, 0.01-0.03 part of vitamin D3, 1-3 parts of trace mineral elements, 0.5-1.5 parts of compound protease, 0.1-0.3 part of a food-grade surfactant, and 100-150 parts of deionized water. The invention relates to the technical field of nutritional supplements. According to the mineral element-containing easy-to-absorb organic calcium powder and the preparation process, vegetable protein is converted into amino acid through biological enzymolysis, the amino acid and calcium carbonate are subjected to a chelation reaction to generate amino acid chelated calcium, and compared with traditional organic calcium, chelated calcium is more easily absorbed by human intestinal tracts; meanwhile, calcium, magnesium and phosphorus are synergistically matched, magnesium can activate vitamin D3, the vitamin D3 further promotes absorption of calcium, phosphorus and calcium form a reasonable proportion, deposition of calcium in bones is enhanced, and calcium ions can be absorbed in intestinal tracts without depending on gastric acid by adopting a bicyclic stable chelating structure.

The invention discloses a processing method of sheepskin food, and belongs to the technical field of food processing. In order to solve the problems that in the existing sheepskin processing, the fishy smell is not thoroughly removed, calcium salt permeation is not uniform due to compact tissues, the taste difference of products is large due to hard outside and soft inside, and the like, the method comprises the following steps: unhairing, washing and dicing fresh sheepskin; putting the skin blocks into a reaction solution containing compound protease and flavourzyme for enzymolysis; transferring the skin blocks subjected to enzymolysis into an aqueous solution containing edible calcium salt and organic acid to be soaked; and finally marinating, cooling, shaping and packaging the skin blocks. According to the method, the fishy smell is effectively removed through composite enzymolysis, tissues are moderately softened, then calcium salt and organic acid coupling embrittlement treatment is performed, a uniform gel network is formed through ionic crosslinking, and the texture of skin blocks is remarkably improved. The method is easy and convenient to operate and mild in condition, and the prepared sheepskin food is low in fishy smell, crisp, tender and uniform in taste, sufficient in flavor permeation and suitable for high-value utilization and industrial production of sheepskin.

The invention relates to a novel amino acid foliar fertilizer and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The method aims at solving the technical problems that in the prior art, the amino acid conversion rate is low, product stability is poor, and severe conditions such as strong acid and strong alkali are possibly used. The method comprises the following steps: mixing soybean meal, fish meal and saccharomyces cerevisiae thalli to form a compound protein raw material, performing two-stage crushing and degreasing, performing step-by-step enzymolysis by adopting a compound enzyme preparation compounded by alkaline protease and flavourzyme, performing enzyme deactivation, and performing ultrafiltration by adopting a ceramic membrane to obtain purified amino acid hydrolysate, and mixing the hydrolysate with a composite trace element mixed solution formed by EDTA-Fe, EDTA-Zn, boric acid, ammonium molybdate and citric acid, adding humic acid, a seaweed extract and an organic silicon penetrant, and carrying out high-speed shearing, filtering and vacuum degassing to finally obtain the product. The method is mainly used for producing the amino acid foliar fertilizer which is high in free amino acid content, good in stability and easy to absorb by plants.

The invention relates to a pharmaceutical composition for repairing oral gingivitis and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises a lactobacillus salivarius fermentation extract, a herba violae extract, golden cicada nymph extract peptide, guava polyphenol and resveratrol; the lactobacillus salivarius fermentation extract is obtained by carrying out aerobic fermentation and anaerobic fermentation on lactobacillus salivarius to obtain a fermentation product, and purifying and extracting the fermentation product through an XAD7HP macroporous adsorption resin column; the philippine violet herb extract is obtained by performing enzymolysis on whole philippine violet herb through pectinase and cellulase and then performing enzymolysis through compound protease to obtain zymolyte, and purifying and extracting the zymolyte through a compound adsorption column. The golden cicada nymph extracted peptide is obtained by purifying and extracting 5kDa zymolyte obtained by performing step-by-step enzymolysis on golden cicada nymphs through papain and trypsin through a Q agarose gel FF adsorption column. All the components are matched for use according to a certain proportion, so that the antibacterial coating is capable of precisely resisting bacteria, has efficient anti-inflammatory and anti-oxidation properties, and is safe and reliable.

The invention relates to a preparation method of compound protein composite gel. The preparation method comprises the following steps: step 1, mixing ball-milled pea starch with water to prepare a starch emulsion; treating to gelatinize the ball-milled pea starch; taking out and cooling to room temperature to obtain pea starch gel, aging and then crushing to obtain pea resistant starch; step 2, dissolving whey protein isolate powder in water to prepare a protein gel stock solution; adding the pea resistant starch according to the proportion of 0.25-1w / v% of the protein gel stock solution; after mixing, continuously stirring at room temperature for 1-3 hours; storing overnight under the condition of a refrigerator at 4 DEG C to ensure that the starch is dissolved with the protein to obtain a gel stock solution for later use; and step 3, taking out the gel stock solution, placing the gel stock solution in a water bath kettle with the temperature of 90-98 DEG C for heat treatment for 20-40 minutes, taking out the gel stock solution, placing the gel stock solution in an ice-water bath for cooling for at least 10 minutes to quickly form gel, and then placing the gel in a refrigerator with the temperature of 4 DEG C for storage. The pea resistant starch is added into the whey protein isolate, so that the synergistic interaction effect on the compound protein gel can also be realized.

The invention discloses a preparation method and application of enzymolysis royal jelly capable of whitening skin, fading spots and resisting wrinkles, and belongs to the technical field of enzymolysis of royal jelly. The technical problem to be solved is that the existing royal jelly zymolyte is lack of research having the effects of whitening, fading spots and resisting wrinkles. The key point of the technical scheme is that the preparation method of the enzymolysis royal jelly comprises the following steps: (1) mixing and homogenizing royal jelly and water, and adjusting the pH value to obtain a royal jelly solution; (2) adding compound protease into the royal jelly solution for enzymolysis, and performing enzyme deactivation and solid-liquid separation to obtain royal jelly enzymatic hydrolysate; the compound protease in the step (2) is fig protease, chymotrypsin and flavourzyme.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The invention discloses goat milk with hypoglycemic activity and a preparation method thereof, and belongs to the technical field of enzyme engineering. The goat milk is used as a raw material, enzymolysis goat milk with alpha-G inhibitory activity is obtained through compound protease hydrolysis, it is found that the enzymolysis goat milk has hypoglycemic activity, and the goat milk powder with the hypoglycemic activity is further prepared through spray drying. The alpha-G inhibitory peptides in the enzymolysis goat milk are separated and identified, 20 alpha-G inhibitory peptides are found from components with the highest alpha-G inhibition rate, the molecular weight of the alpha-G inhibitory peptides is 377-962 Da, and the alpha-G inhibitory peptides are mainly derived from beta-casein, alpha S1-casein, alpha S2-casein, kappa-casein, beta-lactoglobulin, lactoglobulin and osteopontin of the goat milk. The alpha-G inhibitory peptide is prepared by hydrolyzing the goat milk through the compound enzyme, the amino acid sequence of the alpha-G inhibitory peptide is separated and identified, the goat milk with hypoglycemic activity is developed, and theoretical basis and technical support are provided for subsequent development of hypoglycemic dairy products.

The application provides a target-specific virtual screening method and system based on machine learning. Active molecules and inactive molecules are docked with multiple conformations of a target, and protein-ligand interaction features of the docked molecules and related features of the ligands are extracted as input features of a machine learning model. A scoring function model is constructed using multiple machine learning models, and based on the advantages of multiple target-specific scoring function models, an integrated target-specific scoring function model is finally obtained by combining a model integration method. The integrated model can more effectively process complex protein-ligand interaction data, has excellent prediction performance and virtual screening capability, provides more reliable prediction results, and improves the virtual screening capability.

The invention belongs to the field of food science and technology, and discloses high-activity protein powder enriched with seaweed nano polyphenol and a preparation process of the high-activity protein powder. According to the protein powder, the seaweed nano polyphenol with the average particle size of 20-100 nm is prepared by taking preferable brown seaweed as a raw material through the steps of enzymolysis, ultrasonic treatment, membrane separation, high-pressure homogenization and the like. Meanwhile, carrier protein is subjected to heat treatment and enzymatic reaction to form protein nano carrier particles, and seaweed nano polyphenol is encapsulated in the protein nano carrier particles by adopting an electrostatic layer-by-layer self-assembly technology. In addition, protein raw materials are subjected to enzymolysis by compound protease to obtain small molecule peptide fragments, so that the solubility and the digestibility are improved. And finally, mixing a high-activity peptide solution with the protein-polyphenol nano-composite, and freeze-drying. The product has the advantages of high activity, easiness in digestion, no bitter taste and the like.

The invention relates to the technical field of bioengineering and food health care, in particular to anti-aging abalone peptide and a preparation method thereof. The method comprises the following steps: sequentially freezing, freeze-drying and crushing the abalone byproduct to obtain a raw material; mixing the raw materials with water, and boiling to obtain a boiled liquid; cooling the boiled liquid, adjusting the pH value, adding compound protease and an auxiliary agent, carrying out enzymolysis, and carrying out enzyme deactivation after the enzymolysis is finished, so as to obtain an enzymatic hydrolysate; sequentially carrying out rough filtration and microfiltration on the enzymatic hydrolysate to obtain filtrate; separating and purifying the filtrate through a gel filtration chromatography column to obtain a refined abalone peptide solution; and carrying out vacuum concentration and spray drying on the refined abalone peptide liquid to obtain the anti-aging abalone peptide. According to the method, the abalone byproducts are used as raw materials, and the abalone peptide with a definite molecular weight range and excellent free radical scavenging capacity is successfully prepared through systematic processes of boiling, enzymolysis, filtration, separation and purification, concentration and spray drying, so that high-valued utilization of the abalone byproducts is realized.

This invention relates to the field of functional food technology, and discloses a muscle-building, lipid-lowering, and weight-loss composition, its preparation method, and its application. The composition, by weight, comprises: 25-45 parts of complex protein peptides; 5-20 parts of fruit and vegetable powder; 2-8 parts of medicinal and edible extracts; 3-12 parts of complex prebiotics; 1-6 parts of complex vitamins and minerals; 0.5-3 parts of probiotics; 0.5-5 parts of calcium β-hydroxy-β-methylbutyrate; and the remainder being food-acceptable excipients. This invention achieves significant lipid-lowering and weight-loss while effectively increasing lean body mass and muscle mass, and the active ingredients exhibit good stability.

The invention belongs to the technical field of biological medicines, and particularly relates to a compound protein preparation for preventing and treating avian influenza and a preparation method thereof. The compound protein preparation is prepared from the following raw materials in parts by weight: 5 to 10 parts of immune globulin, 15 to 19 parts of radix puerariae, 10 to 15 parts of buchnera macrocarpa, 8 to 12 parts of dendranthema morifolium, 7 to 10 parts of honeysuckle flower, 3 to 10 parts of radix astragali seu hedysari, 2 to 8 parts of echinacea purpurea, 1 to 7 parts of semen cuscutae and 2 to 5 parts of liquorice root. By taking the compound protein preparation for preventing and treating the avian influenza, clinical symptoms caused by the avian influenza can be effectively relieved, and the health level of animals is improved; the vaccine-induced immune response can be effectively enhanced, so that the avian influenza antibody level of animals is improved, the avian influenza is effectively prevented and treated, and the vaccine has a wide application prospect.

To provide a CRISPR gene delivery system that ensures robust expression of CRISPR proteins and avoids the risk of genomic integration. [Solution] A synthetic, non-infectious, self-replicating RNA vector encoding a CRISPR protein is provided. Each self-replicating RNA vector contains sequences encoding multiple non-structural replication complex proteins derived from alphaviruses, and sequences encoding a CRISPR protein. A method for genome editing is also provided, which involves introducing a synthetic self-replicating RNA vector into a cell along with at least one corresponding guide RNA.

The invention discloses hypoglycemic peptide sheep milk powder and a preparation method thereof, and belongs to the technical field of enzyme engineering. According to the preparation method, sheep milk is taken as a raw material, the inhibition rates of different proteases on dipeptidyl peptidase IV (DPP-IV) are compared after the sheep milk is subjected to enzymolysis by different proteases, the optimal compound protease is obtained, then the hypoglycemic peptide sheep milk is obtained through hydrolysis of the compound protease, the hypoglycemic peptide sheep milk has DPP-IV inhibition activity, and the sheep milk powder with the hypoglycemic activity is further obtained through spray drying. The DPP-IV inhibition rate is 69.44% + / -2.66%-87.45% + / -1.25%, and the compound can be used for assisting in reducing blood sugar of consumers and reducing the dosage of hypoglycemic peptides so as to reduce side effects. The hypoglycemic peptides in the enzymolysis sheep milk are separated, purified and identified through ultrafiltration, preparative chromatography and HPLC-MS / MS, 12 hypoglycemic peptides from sheep milk protein are obtained, and theoretical and technical supports are provided for development of hypoglycemic assisting dairy products.

The invention discloses a scallop skirt umami peptide as well as a preparation method and application thereof. The preparation method comprises the following steps: cleaning fresh scallops, opening shells, taking skirts, removing visceral clusters, adding water, and homogenizing to obtain scallop skirt homogenate; adding water into the scallop skirt homogenate, sequentially adding flavourzyme and compound protease, performing enzymolysis, performing enzyme deactivation after enzymolysis, performing centrifugation, collecting supernate, performing vacuum concentration, and performing freeze drying to obtain scallop skirt zymolyte; separating and purifying the scallop skirt zymolyte by adopting a Sephadex LH-20 sephadex column to obtain a sephadex column separation component containing the scallop skirt umami peptide; performing LC-MS / MS identification on the sephadex column separation component containing the scallop skirt umami peptide to obtain an amino acid sequence of the scallop skirt umami peptide; the scallop skirt umami peptide is verified by using a molecular docking technology and sensory evaluation. The prepared scallop skirt umami peptide has good umami, has great application significance in freshness enhancing food additives, is a small-molecular-weight peptide, and is easy to absorb by human bodies.

The invention relates to the technical field of biological detection, in particular to a dry-state hydrogel-based plasma-Fabry-Perot cavity biological detector as well as a preparation method and application thereof. Compared with ELISA and other immunological detection methods depending on chemical tags and signal amplification, the method does not need a signal amplification process and shortens the detection time. The integrated plasma interlayer thickness sensing structure provided by the invention realizes direct signal reading after sample incubation is finished. According to the invention, the hydrogel is used as a sensing interlayer, and the hydrogel is used as a three-dimensional medium, so that a larger thickness change can be generated in a spatial scale. The biological detector can still keep relatively high specificity in a complex protein liquid phase environment (serum). The device has the structural characteristics of chip formation and miniaturization, and can be used for detecting various types of high-throughput proteins at the same time.

The invention discloses a walnut source DPP-IV inhibitory peptide as well as a preparation and screening method and application thereof. The walnut source DPP-IV inhibitory peptide comprises FPAG and / or LPSYQPTP, the preparation and screening method comprises the following steps: (1) adding walnut protein powder into deionized water, and fully dissolving to obtain a walnut protein solution; (2) adding hydrolyzed compound protease into the walnut protein solution for enzymolysis to obtain walnut protein hydrolysate freeze-dried powder; (3) re-suspending the walnut protein hydrolysate freeze-dried powder in deionized water, and performing ultrafiltration separation by using an ultrafiltration membrane with the molecular weight cutoff of 1kDa to obtain a component F1; step (4), performing peptide fragment sequence identification on the obtained F1 component through peptidomics; and step (5), screening from peptide fragment sequences obtained by identification through a bioinformatics technology. The walnut source DPP-IV inhibitory peptide screened by the invention can be used for preparing a medicine or food with a DPP-IV inhibition function, so that the problems that the existing walnut source DPP-IV inhibitor has many side effects and the like are solved.

The invention discloses a composition capable of being used for body weight management and a preparation method and application thereof, and belongs to the technical field of foods.The composition is prepared from, by weight, 15-50 parts of compound protein powder solid beverage, 2-15 parts of quinoa flour and 1-8 parts of compound fruit and vegetable powder. The quinoa flour is prepared through high-pressure homogenization, composite enzymolysis, ethanol extraction and supercritical extraction. Experiments prove that the chenopodium quinoa flour prepared by the method has more advantages in the aspect of controlling weight management, and the goals of enhancing satiety and reducing total calorie intake are achieved while nutrition balance is guaranteed through the combined action of the chenopodium quinoa flour, the compound protein powder solid beverage and the compound fruit and vegetable powder.

The invention discloses an aquatic protein feed and a preparation method thereof, and belongs to the technical field of aquaculture feeds, the feed takes enzymolysis hermetia illucens protein, wall-broken yeast protein and enzymolysis pea peptide as a compound protein source, and comprises a multilayer sustained-release microcapsule prepared by adopting a three-fluid electrostatic spraying technology, and probiotics and digestive enzymes are embedded in the multilayer sustained-release microcapsule, so that the aquatic protein feed is prepared. And the outer layer is a pH response type gel and a protective film. The preparation method comprises the following steps: carrying out enzymolysis and membrane separation pretreatment on a compound protein source, constructing the microcapsule by virtue of low-temperature electrostatic spraying, and carrying out low-temperature mixing extrusion and functional post-treatment under the protection of inert gas. According to the invention, time-sequence precise release and efficient protection of nutrients and active ingredients in digestive tracts are realized, the protein digestibility can be obviously improved, the feed coefficient and nitrogen emission are reduced, and the feed has the comprehensive functions of promoting growth, regulating intestinal tracts and enhancing immunity at the same time; the industrial problems that a traditional feed is high in dependence degree on fish meal and low in digestion and utilization rate, and functional additives are prone to inactivation are effectively solved.

The application discloses a gene editing system, a double-plasmid system, engineering bacteria and application, and relates to the technical field of gene editing. The gene editing system comprises gRNA and a recombination system, and the recombination system comprises a nucleotide sequence shown in SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3. The gene editing system provided by the application has good controllability, high gene editing efficiency, and can realize efficient gene editing under the condition of short homologous arms. The gene length of the recombination system is far less than that of a lambda-red system and a RecET system, and the recombination system does not depend on complex protein cooperation, is a kind of compact and efficient and broad-spectrum recombination system of gene cluster. In addition, the recombination system has good portability, good host compatibility, can realize efficient gene editing in host bacteria, has the modularization characteristics of 'plug and play', is convenient to operate, does not need to be passed through multiple generations, and does not need to be induced in advance.

The present application provides a composition with satiety and weight control and anti-aging, and a preparation method and application thereof. The composition comprises the following raw materials by weight: polysaccharide 11-13 parts, fiber powder 20-25 parts, highland barley powder 12-18 parts, compound powder 18-23 parts, pectin 4-8 parts, ganoderma lucidum powder 6-9 parts, and compound protein 8-12 parts, and relates to the technical field of satiety and weight control and anti-aging compositions. The composition can form an effect of hindering nutrient absorption or prolonging gastric emptying, increase the volume of intestinal contents to achieve satiety, thereby achieving the functions of well controlling weight gain and reducing body weight. In addition, the composition can activate the body's antioxidant enzyme system, improve the activity of superoxide dismutase (SOD), and reduce the content of malondialdehyde (MDA), thereby delaying cell aging and achieving the anti-aging effect.

This invention discloses a method for preparing regenerated sweet potato rice for ice cream production, comprising washing, grinding, enzymatic hydrolysis, conditioning, and granulation steps. The enzymatic hydrolysis step involves rapidly adding washed fresh sweet potatoes, rice, soybeans, wheat, corn, and oats in a mass ratio of 100:(100-130):(35-46):(25-35):(15-25):(10-25) to a grinder for grinding, obtaining a composite slurry. The mixed slurry accounts for approximately 35-50% of the total mass. A composite enzymatic hydrolysate is added at 0.7-1.5% of the total mass for enzymatic hydrolysis, and the mixture is stirred at 45°C for 5 hours. The composite protease includes amylase, neutral protease, and alkaline protease. The stirring speed is controlled at 300 rpm. The enzymatic hydrolysate is then heated to 85°C and maintained for 20 minutes to obtain an enzyme-inactivated mixed slurry. The preparation technique involves cooling the slurry to 50-65°C, adding the slurry, dietary fiber powder, brown rice flour, and trace elements, mixing them in a ratio of 100:(30-40):(10-30):(1-10), and controlling the temperature at 37-45°C. The regenerated rice prepared according to this invention combines the texture, flavor, and chewiness of ice cream sweet potato. The nutrients and proteins in the rice are more easily absorbed by the human body through enzymatic hydrolysis, increasing the nutritional value of the regenerated rice.

The application discloses a composition capable of being used for weight management and a preparation method and application thereof, and belongs to the field of food technology.The composition is composed of the following raw materials in parts by weight: 15-50 parts of composite protein powder solid beverage, 2-15 parts of quinoa powder and 1-8 parts of composite fruit and vegetable powder; the quinoa powder is prepared through high-pressure homogenization, composite enzymolysis, ethanol extraction and supercritical extraction. Experiments prove that the prepared quinoa powder has more advantages in weight control management, and meanwhile, the three of the quinoa powder, the composite protein powder solid beverage and the composite fruit and vegetable powder are used to jointly ensure balanced nutrition, enhance satiety and reduce total calorie intake.

The invention discloses a muscle-building dietary flavone compound protein composition as well as a preparation method and application thereof, and belongs to the field of dietary supplements. The composition is prepared from the following components in parts by weight: 50 to 80 parts of concentrated whey protein powder, 5 to 20 parts of separated whey protein powder, 0.01 to 3 parts of hydrolyzed whey protein powder, 0.1 to 1 part of bovine colostrum powder, 0.01 to 0.05 part of kaempferol and 0.01 to 0.1 part of glucomannan. The composition can improve the muscle synthesis and growth rate, inhibit muscle reduction caused by sarcopenia, improve the muscle oxidation resistance, accelerate the muscle repair speed and activate the protein synthesis rate-limiting enzyme AKT and the growth kinase ERK. The composition can be used for preparing food, special diet and health food.

This invention discloses a method for preparing Hu sheep bone polypeptides, belonging to the field of polypeptide preparation technology. Specifically, it involves pretreating fresh Hu sheep bones to obtain Hu sheep bone powder, and then enzymatically hydrolyzing the Hu sheep bone powder to obtain Hu sheep bone polypeptides. The enzymatic hydrolysis uses a complex protease, including trypsin and papain, with a mass ratio of trypsin to papain of 1:0.1-10. The enzymatic hydrolysis is purified using silica gel column separation. The silica gel column packing material includes silica gel or modified silica gel. In the preparation of modified silica gel, the silica gel is first activated with acid, then functionalized with 3-aminopropyltriethoxysilane, and then reacted with a modified monomer, cyclohexylvinyl ether, to obtain modified silica gel. This invention also discloses a method for preparing Hu sheep bone polypeptides with high hydroxyl radical scavenging rate, high calcium chelation rate, and good cell proliferation effect after calcium chelation.

The invention relates to the technical field of functional food processing, in particular to a high-protein high-fiber NAD < + > activated meal replacement bar and a preparation method thereof. The feed comprises the following components in parts by weight: 25-35 parts of animal protein, 10-15 parts of vegetable protein, 15-25 parts of soluble dietary fiber, 6-10 parts of chenopodium quinoa expanded balls, 4-8 parts of coix seed powder, 4-8 parts of Chinese yam powder, 3-5 parts of chia seeds, 4-6 parts of medium-chain fatty acid, 10-15 parts of isomaltooligosaccharide syrup, 0.4-1.3 parts of a pH regulator, 2-4 parts of triglyceride, 4.2-7 parts of sorbitol and 8-12 parts of water. A cold processing technology is adopted to carry out staged mixing and staged gradient cold pressing processing forming, a fence technology is applied to construct a triple barrier, and ultraviolet sterilization post-treatment is carried out, so that the meal replacement bar is prepared. The method has the advantages that a compound protein system is adopted, and PDCAAS score and water binding capacity are both considered; the compactness and the structural uniformity of the product are improved; and the production process is optimized.