321 results about "Complex protein" patented technology

The present invention provides methods of screening for compounds that interfere with the interaction between ERG. ETV1, ETV4 or ETV5 and mSWI / SNF (BAF) chromatin remodeling complex proteins. Included are methods of screening for compounds that interfere with the interaction between ERG, ETV1, ETV4 or ETV5 and BAF155. Methods of treating prostate cancer with compounds that interfere with the interaction between ERG, ETV1, ETV4 or ETV5 and mSWI / SNF (BAF) chromatin remodeling complex proteins are also provided.

The invention relates to the related technical field of extraction of active ingredients in roselle calyx, and particularly provides an extract based on roselle oil and roselle polypeptide as well as an extraction method and application of the extract. The extraction method comprises the following steps: S1, sequentially performing crushing treatment and supercritical fluid extraction treatment on roselle calyx to obtain a roselle oil extract and flower residues; s2, the roselle residues are sequentially subjected to fermentation treatment and enzymolysis treatment, and a roselle polypeptide extract is obtained; wherein a strain used in the fermentation treatment is selected from at least one of saccharomyces cerevisiae, bacillus subtilis and lipomyces starkeyi; an enzyme used in the enzymolysis treatment is selected from at least one of acid protease and compound protease; the extract comprises the roselle oil extract and the roselle polypeptide extract. The extraction method provided by the invention improves the extraction rate of active ingredients extracted from roselle calyx.

The invention provides a pumpkin seed protein peptide for improving vascular endothelial cell dysfunction and a preparation method thereof, and relates to the technical field of bioactive peptides, the pumpkin seed protein peptide is obtained by enzymolysis of pumpkin seed crude protein with compound protease; the compound protease comprises alkaline protease and trypsin; the mass ratio of the alkaline protease to the trypsin is (1: 3)-(3: 1), and compared with other enzymolysis modes, the pumpkin seed protein peptide prepared by performing enzymolysis on the pumpkin seed crude protein by adopting the alkaline protease and the trypsin has the advantage that the capability of scavenging peroxide free radicals is improved; and the arginine is enriched. The form of peptide in the pumpkin seed protein peptide conforms to the characteristics of high-activity antihypertensive active peptide; the traditional Chinese medicine composition has a relatively good improvement effect on vascular endothelial cell dysfunction and can promote the generation of NO factors. The technical problem that in the prior art, no bioactive peptide capable of improving vascular endothelial cell dysfunction exists is solved.

The preparation method of the composite soybean protein gel comprises the following steps: adjusting the pH value of an egg white protein solution, carrying out heat preservation, adding protein glutaminase to carry out hydrolysis reaction, centrifuging, collecting supernate, and freeze-drying to obtain deamidated egg white protein; adding TG enzyme into the soybean protein isolate and deamidated egg white protein solution for enzymatic cross-linking reaction, centrifuging after enzyme deactivation, collecting supernate, and freeze-drying to obtain compound protein powder; dissolving the composite protein powder in water, stirring, and heating to form composite soybean protein gel; according to the preparation method disclosed by the invention, glutamine in the egg white protein is hydrolyzed by using the protein glutaminase, so that only the site of lysine is reserved, and then TG enzyme treatment is performed, so that the crosslinking of the egg white protein is avoided, and the content of disulfide bonds in the soybean protein is increased, thereby remarkably improving the gel strength and compression resistance of the composite soybean protein thermal gel.

The invention provides an intelligent response type medical liquid application and a preparation method thereof, and relates to the technical field of medical dressings. The liquid application is composed of a basic repairing component, a natural antibacterial component, a plant active component, an intelligent carrier system and auxiliary materials. Accurate release of active ingredients is realized by adopting a pH / temperature double-response intelligent delivery system, and the transdermal absorption rate is remarkably improved; wound healing is accelerated through the synergistic effect of the compound protein and the plant extract; active ingredients are effectively reserved through a multi-mode sterilization process. The liquid application disclosed by the invention has the characteristics of strong antibacterial property, good environmental adaptability, excellent healing effect and the like.

The invention relates to the technical field of biology, in particular to a method for preparing a high-activity small-molecule cartilage extract from animal cartilage tissue, the cartilage extract and application of the cartilage extract, and the method for preparing the high-activity small-molecule cartilage extract from the animal cartilage tissue comprises the following steps: S1, soaking; s2, bone breaking; s3, removing cartilage fat by adopting a skimming and centrifugal separation process; s4, enzymolysis: performing compound shearing on compound protease to obtain enzymatic hydrolysate; s5, filtering; and S6, freeze drying. Through the application of various new technologies, the content of type II collagen in the prepared cartilage extract reaches 65% or above, the content of GAGS reaches 30% or above, the content of hyaluronic acid reaches 17% or above, and the problem that in the prior art, biological activity is damaged due to the processes of degreasing through a chemical degreasing agent, physical high temperature, chemical degradation and high-temperature drying is solved; the biological activity of the natural extract is completely reserved by adopting a physical degreasing and multi-enzyme multi-level directional composite enzymolysis technology and a freeze-drying technology at the temperature of minus 40 DEG C or below.

The invention provides ACE (Angiotensin Converting Enzyme) inhibitory peptide, middle-aged and elderly formula goat milk powder and a preparation method thereof, goat milk is taken as a raw material, the goat milk is sterilized and cooled, compound protease consisting of three proteases is inoculated for constant-temperature enzymolysis, and the ACE inhibitory peptide is obtained through ultrafiltration, preparative chromatographic separation and freeze drying. And uniformly mixing the active peptide with a nutrition enhancer and goat milk powder required by the middle-aged and elderly people to obtain the active peptide goat milk powder for the middle-aged and elderly people. Or after the goat milk is subjected to enzymolysis by compound protease, directly adding a nutrition enhancer required by the middle-aged and elderly people, and then concentrating and spray-drying to obtain the formula goat milk powder for the middle-aged and elderly people. The ACE inhibitory peptide prepared by the invention can be used as a food functional factor, and the prepared formula goat milk powder for middle-aged and elderly people contains the ACE inhibitory peptide and has the function of assisting in lowering blood pressure.

The invention relates to the field of biological medicine, and provides a preparation method of a fermentation product containing giant salamander peptide, which comprises the steps of stepped enzymolysis pretreatment, staged inoculated fermentation, synergistic enzymolysis and molecular weight grading purification. The method comprises the following steps: firstly, carrying out staged enzymolysis on giant salamander tissues by utilizing pepsin, trypsin and flavourzyme to obtain a primary peptide solution; then, bacillus subtilis, lactobacillus plantarum and bifidobacterium are adopted for directional fermentation, and flora metabolism is optimized through dissolved oxygen control and pH automatic regulation. Then, compound protease is added for secondary enzymolysis, so that the yield of the small molecule peptide is increased. And finally, carrying out ultrafiltration and nanofiltration graded purification, vacuum concentration, freeze drying and gamma-ray sterilization to obtain the micromolecular giant salamander peptide. The molecular weight of the obtained product is mainly distributed within 0.5-5 kDa (lt), and the proportion of 3 kDa is larger than or equal to 80%), the DPPH free radical scavenging rate is larger than or equal to 85%, the ABTS < + > scavenging rate is larger than or equal to 80%, and the obvious anti-oxidation and auxiliary liver protection effects are achieved.

The invention discloses a spirulina platense skin extract as well as a preparation method and application thereof, and relates to the technical field of biomedicine. The obscured spirulina skin extract is rich in various active ingredients including collagen family members, viscose protein, C1Q complement protein, fibronectin and the like, and the ingredients complement and promote one another, so that the extract has remarkable effects in the aspects of skin aging resistance, skin injury repair and the like; therefore, the method has a wide application prospect in medicine research and development, cosmetics and functional foods. According to the extraction method of the skin protein of the obtuse spirulina, the sample is subjected to rapid freeze thawing treatment, and the steps of mechanical crushing, ultrasonic-assisted separation and multiple filtration are combined, so that the influence on the activity of the product is avoided, and various compound proteins can be obtained; the pH value of a buffer solution is adjusted, the property of the extract is further optimized, and the extract is more suitable for human skin, is sterile and can be directly used as a product raw material, so that large-scale production is realized, and industrial application of the obtuse spirulina skin extract is promoted.

The invention provides a fructus cannabis protein peptide capable of reducing blood sugar and regulating glycometabolism and a preparation method and application thereof, and belongs to the technical field of functional active peptides, and the preparation method comprises the following steps: performing low-temperature supersonic fluid crushing on fructus cannabis, performing enzymolysis pretreatment on the crushed fructus cannabis, adding compound protease for enzymolysis, adding PNGaseF enzyme for further enzymolysis, and performing freeze drying to obtain the fructus cannabis protein peptide capable of reducing blood sugar and regulating glycometabolism. And carrying out membrane ultrafiltration interception and enrichment on the enzymolysis supernatant, carrying out tangential flow filtration, concentrating, drying and sterilizing to obtain the fructus cannabis protein peptide. Tests prove that the novel fructus cannabis protein peptide has the following remarkable effects that the fasting blood glucose level can be remarkably reduced, and uncomfortable symptoms such as thirst, polydipsia, diuresis and weakness caused by hyperglycemia are relieved; the symptoms of glucose intolerance and insulin resistance caused by hyperglycemia can be obviously relieved, and the onset risk of type 2 diabetes mellitus is reduced; the level of fasting serum insulin and glycosylated hemoglobin can be obviously reduced, and the level of serum GLP-1 can be improved.

The invention discloses a high-protein-content enzymolysis corn protein powder processing method which comprises the following steps: S1, pretreatment: pretreating corn protein powder to obtain low-starch-content wet corn protein powder; s2, enzymolysis: starch in the corn protein powder is removed, the enzymolysis comprises I-segment enzymolysis and II-segment enzymolysis, one compound protease is firstly used for I-segment enzymolysis, and then the other compound protease is used for II-segment enzymolysis; and S3, spray drying: finally, spray drying is performed to generate the sectional type enzymolysis processing corn protein powder of the product, on one hand, the starch content in the corn protein powder is reduced, and the dosage limitation of the corn protein powder in aquatic feed is relieved; on the other hand, the corn protein powder is modified and added, most of prolamin and glutelin in the raw materials are changed into water-soluble protein and small peptide, and the animal utilization rate is greatly increased; in addition, the product can partially replace fish meal, so that feed resources are expanded, dependence on the fish meal is reduced, and the cost of an aquatic feed formula is also reduced.

The invention discloses pericarpium citri reticulatae compound protein powder as well as a preparation method and application thereof. The protein powder takes pericarpium citri reticulatae extract microcapsules, seville orange flower extract microcapsules, soybean protein isolate powder, polydextrose, resistant dextrin, soybean peptide powder, tremella powder and the like as main components, and further comprises edible salt, edible essence, a nutrition enhancer, a sweetening agent and an anti-caking agent. The preparation method comprises the steps of raw material weighing, premixing, high-speed shearing and mixing and the like. Active components of the dried orange peel and seville orange flower extract are extracted through an ultrasonic-assisted ethanol extraction method, and sodium alginate and chitosan are adopted for microencapsulation treatment, so that the stability and solubility of the dried orange peel and seville orange flower extract are improved. The compound protein powder disclosed by the invention has the functions of maintaining muscle mass and relieving symptoms such as nausea and abdominal distension in a weight loss process, has good storage stability and safety, and is suitable for being taken for a long time.

The invention discloses a preparation method and application of squid collagen peptide with beauty maintaining and young keeping effects, and belongs to the technical field of marine food processing. The collagen is obtained through enzymolysis hydrolysis, the structure of the collagen is kept, damage to amino acid is avoided, treatment and use of acid, alkali and organic solvents are reduced in the preparation process, and equipment loss and environmental pollution are avoided. The second enzymolysis is performed by adopting the synergistic effect of the compound protease, the low molecular weight, degreasing and bitterness removal of the peptide powder can be realized, and the obtained peptide powder is low in molecular weight, good in flavor, free of peculiar smell and suitable for being eaten, digested and absorbed by human bodies. Experiments prove that the squid collagen peptide prepared by the preparation method disclosed by the invention has relatively strong oxidation resistance and has remarkable effects in the aspects of whitening, wrinkle resistance, oxidation resistance and the like.

The invention relates to the technical field of medical care, and particularly discloses a preparation method and application of sea cucumber peptide. The preparation method of the sea cucumber peptide provided by the invention comprises the following steps: heating sea cucumber primary pulp for denaturation, and then adding compound protease for enzymolysis treatment to obtain sea cucumber enzymatic hydrolysate; the addition amount of the compound protease is 0.1-0.15 mg / mL, and the compound protease is prepared from papain, ficin and trypsin according to the weight ratio of 1: (0.5-1.5): (2-3); adding activated carbon and diatomite into the sea cucumber enzymatic hydrolysate, and performing enzyme deactivation, filtration and concentration treatment to obtain sea cucumber peptide; the invention also provides application of the sea cucumber peptide obtained by the preparation method in medicines and foods. According to the preparation method of the sea cucumber peptide, the sea cucumber peptide with small molecular weight and good uniformity can be obtained, and the prepared sea cucumber peptide tablet candy has the effects of relieving physical fatigue, resisting aging, resisting oxidation and the like.

The invention belongs to the technical field of bioengineering, and discloses a sunflower seed peptide for regulating metabolism of brain cells, the sunflower seed peptide has a biological sequence of Arg His Ala Lys Thr Pro Ser Asn Lys, and a preparation method of the sunflower seed peptide for regulating metabolism of brain cells comprises the following specific steps: S1, raw material pretreatment; s2, peeling and crushing; s3, protein extraction; s4, performing enzymolysis treatment; s5, performing enzyme deactivation treatment; s6, filtering and purifying; s7, concentrating and drying; and S8, quality detection and packaging. The sunflower seed protein is directionally decomposed into the active peptide fragment by precisely regulating and controlling compound protease and enzymolysis conditions, the active peptide fragment can be compatible with a brain cell specific receptor and penetrate through a blood brain barrier, and cell experiments and animal models prove that the peptide fragment can activate a mitochondrial respiratory chain complex, so that the mitochondrial membrane potential is increased by more than 30%, and the mitochondrial membrane potential is increased by more than 30%. Meanwhile, the activity of neurotransmitter synthetase can be enhanced, the brain health is protected, and tests further prove that the sunflower seed peptide also has the function of regulating the metabolism of brain cells.

The invention discloses a functional feed formula for improving endurance and reproductive performance of donkeys, and relates to the technical field of feed formulae, the functional feed formula comprises selection and pretreatment of raw medicinal materials, nutrient element proportioning, treatment of functional reinforcing materials and treatment after molding, the functional reinforcing materials comprise a functional formula A and a functional formula B, the method has the advantages that the mixed leaves are subjected to low-temperature freeze-drying treatment and then crushed into powder, bioactive components such as protein and flavonoids in the leaves are reserved, the leaves can be used as a high-quality plant protein source and a functional component source, carbon dioxide gas is introduced into the compound protein leaf powder for activation, and the compound protein leaf powder can be used as a high-quality plant protein source and a functional component source. The method has the advantages that bioactive components in the leaf powder can be activated, the bioavailability of the leaf powder can be increased, polysaccharide in the high-sugar Chinese velvet herb can be further decomposed into monosaccharide and oligosaccharide by saccharifying the high-sugar Chinese velvet herb, and the energy release speed can be increased.

The invention provides collagen tripeptide liquid with moisturizing and anti-photoaging effects and a preparation method thereof, and relates to the technical field of bioactive peptides.The preparation method comprises the steps that pretreatment and first-time enzymolysis are conducted, specifically, pretreated fish skin and water are mixed, the pH value is adjusted, compound protease is added for enzymolysis, and first enzymatic hydrolysate is obtained; second-time enzymolysis: adjusting the pH value of the first enzymatic hydrolysate to 6.0-7.5, adding neutral protease for enzymolysis, and then adding aminopeptidase for enzymolysis for 20-40 minutes to obtain second enzymatic hydrolysate; and performing enzyme deactivation, decoloration, separation and purification to obtain collagen tripeptide liquid. Wherein the content of collagen tripeptide, the content of glycine-proline-hydroxyproline and the content of hydroxyproline are high, and good moisturizing and anti-photoaging effects are achieved.

The invention discloses a preparation method of a lactoferrin-polyphenol nano compound with an intestinal immune function. The method comprises the following steps: firstly, moderately hydrolyzing lactoferrin under the action of compound protease, and optimizing a protein molecular structure; then, compounding with water-soluble polyphenol (such as chlorogenic acid or epicatechin) by utilizing a metal ion chelating technology, and inducing lactoferrin to be bridged and aggregated by Ca to form a compound; and then, carrying out ultrasonic homogenization treatment, and further controlling the particle size to enable the particle size to be distributed in a range of 100-300 nm. The obtained compound is subjected to nanofiltration concentration and electrostatic spray drying to obtain a shell-core nano compound with a stable structure, and nitrogen-filled packaging is adopted to prevent oxidation. The lactoferrin-polyphenol nano-composite prepared by the invention not only has good stability and intestinal immunity enhancement effect, but also has remarkable antibacterial activity, and can be widely applied to functional foods and nutritional health-care products.

The invention discloses a salty and fresh difunctional taste-active peptide as well as a preparation method and application thereof, and relates to the technical field of biology. The amino acid sequence of the salty and fresh difunctional taste-active peptide is as shown in any one of SEQ ID NO.1-4. According to the invention, a compound protease enzymolysis method is adopted to prepare enzymatic hydrolysate rich in salty and fresh dual-function taste-active peptides, a plurality of salty and fresh dual-function taste-active peptides are identified and screened out by utilizing polypeptiomics and a molecular docking technology, and meanwhile, a transmembrane structure domain of a taste receptor is evaluated and predicted; chemical bonds and docking sites for docking of the salty and fresh difunctional taste-active peptide and the taste receptor are analyzed, key chemical bonds and binding sites for interaction of the salty and fresh difunctional taste-active peptide and the salty receptor are summarized, and the salty taste and umami strength of the salty and fresh difunctional taste-active peptide and the salty receptor are evaluated. The salty and fresh dual-function flavor peptide screened by the invention has strong salty taste and fresh taste, can be used as a substitute of sodium salt, and is used for preparing low-salt and low-sodium food. The invention also provides a certain reference for rapidly and simply obtaining the salty and fresh dual-function taste-active peptide.

The invention discloses oyster-soybean compound protein gel constructed based on low-temperature synergy as well as a preparation method and application of the oyster-soybean compound protein gel, and belongs to the technical field of functional foods. The composite gel is formed by oyster protein and soybean protein isolate through low-temperature stabilization, medium-temperature construction and thermal induction treatment. The preparation method comprises the following steps: preparing an oyster protein suspension; preparing a soybean protein isolate suspension; the oyster protein suspension and the soybean protein isolate suspension are mixed, stirred and left to stand, a gel structure is preliminarily constructed and then subjected to thermal induction curing treatment, and after the thermal induction curing treatment is finished, the oyster-soybean compound protein gel is formed through storage at low temperature and can be used in the field of food processing. The oyster protein and the soybean protein isolate are combined, so that the composite gel has excellent gel strength, smooth texture and long-acting storage stability, no organic chemical reagent or food additive is used in the preparation process, the formula safety is ensured, the process is simple and convenient, and the composite gel shows huge application value and industrialization prospect.

The invention relates to a preparation method and application of collagen tripeptide liquid, and belongs to the technical field of bioactive peptides, the preparation method comprises the following steps: A1, pretreating fish skin raw materials to obtain fish skin homogenate; a2, the pH value of the fish skin homogenate is adjusted to be neutral, compound protease accounting for 1-4% of the mass of the fish skin is added for enzymolysis, and a collagen peptide crude extracting solution is obtained; a3, performing enzyme deactivation and centrifugation, collecting supernate, and performing nanofiltration to obtain a filtered component 1 and an intercepted component 1; a4, putting the interception component 1 into a hydrochloric acid solution, and hydrolyzing to obtain hydrolysate; adjusting the hydrolysate to be neutral, and then carrying out nanofiltration to obtain a filtered component 2 and an intercepted component 2; and A5, mixing the filtered component 1 and the filtered component 2, carrying out nanofiltration, and collecting an intercepted component 3 to obtain a collagen tripeptide solution. The collagen tripeptide in the prepared collagen tripeptide liquid is high in yield, and the purity is also effectively improved.

The invention discloses a method for improving the texture characteristics of canned abalone. After fresh abalone is taken out, cleaned, water-controlled, shaped and salt-ground, composite protease is used to decompose collagen and elastin in the abalone meat and hydrolyze connective tissue fibers. At the same time, ultrasonic treatment is used to destroy the original muscle structure, which effectively reduces the hardness value of the abalone meat. After the treatment, the abalone meat is cleaned, water-controlled, blanched, tooth-removed and graded, and then placed in a 500V DENBA+ electrostatic wave preservation system and treated at 4°C for 1h. After that, the abalone meat is subjected to metal detection, weighing and canning, vacuum sealing, can washing, sterilization and cooling, and drying. The canned abalone treated by the above steps can improve its rubber meat texture characteristics to the greatest extent, effectively ensure the moisture of the abalone meat, and maintain its nutritional components, good flavor and taste.

The invention discloses a method for simultaneously extracting starch and protein and application thereof. The invention belongs to the technical field of corn deep processing. The invention aims to solve the technical problems that the existing method is low in production efficiency and the obtained corn starch and corn protein are poor in processing characteristic and digestion characteristic. The method comprises the following steps: (1) performing vacuum low-temperature alkalization cooking on corn kernels in a calcium hydroxide solution; (2) performing ultrasonic-high static pressure cooperative treatment after cooking, and then soaking; (3) performing coarse grinding on the soaked corn kernels, then performing fine grinding, and performing enzymolysis with compound protease; and (4) finely grinding, filtering, standing, centrifuging, washing with water, precipitating, respectively collecting a lower-layer white part and an upper-layer yellow part, and freeze-drying to respectively obtain the corn starch and the corn protein. The method improves the processing characteristics of starch and the digestion characteristics of protein, and can be applied to development and production of high-quality corn starch or corn protein-based food.

The invention belongs to the technical field of cosmetics, and particularly relates to ultrasonic-assisted composite protease hydrolyzed pea protein as well as a preparation method and application thereof. The preparation method comprises the following steps: raw material treatment, high-temperature sterilization, ultrasonic enzymolysis of a compound protease mixed solution, enzymatic hydrolysate separation, supernate separation, spray drying and obtaining of a target product pea peptide, pea protein is hydrolyzed by compound protease to obtain the pea peptide with the molecular weight of 6000u or below and an anti-wrinkle function, and enzyme deactivation treatment is performed at high temperature to obtain the pea peptide with the anti-wrinkle function. Filtering by using an ultrafiltration fiber membrane, separating from the inactivated enzymatic hydrolysate, drying by using a spray dryer to prevent oxidation in the drying process, and hydrolyzing the pea protein by using a compound enzyme to obtain the pea protein peptide; the obtained product easily and directly enters deep skin through the surface layer of the skin, is quickly absorbed by the skin of a human body to play a role, and has higher biological activity and bioavailability. The anti-wrinkle peptide is prepared from pea protein, resources can be fully utilized, waste is turned into wealth, and waste of raw materials is reduced.

The invention discloses a polypeptide fertilizer beneficial to cold resistance of crops and a preparation method thereof, and belongs to the technical field of agricultural fertilizers, the fertilizer is prepared by taking livestock and poultry blood (preferably pig blood) as a raw material, preparing high iron protein peptide through a compound protease hydrolysis process, and matching with a trace element compound, humic acid and a carrier. The preparation process comprises the steps of enzymolysis preparation of the ferritin peptide, fertilizer mixing, granulation, drying and the like. The polypeptide fertilizer disclosed by the invention takes the high-iron protein peptide as a core, the contained small-molecular amino acid polypeptide can enhance the antioxidant enzyme activity of crops at low temperature and reduce malonaldehyde accumulation, the porphyrin iron can promote chlorophyll synthesis and energy metabolism, and the high-iron protein peptide and the porphyrin iron cooperate to remarkably improve the cold resistance of the crops. Meanwhile, by cooperating with trace elements and humic acid, the cold resistance is enhanced, nutrient absorption of crops is promoted, the yield and quality are improved, multiple effects of resisting cold, improving quality, increasing yield and the like are achieved, the raw materials are wide in source, the cost is low, the preparation process is simple, and large-scale production and application are facilitated.

The invention relates to the technical field of agricultural fertilizers, in particular to an enzymolysis fish protein type amino acid-containing water-soluble fertilizer and a preparation method thereof, the fertilizer is prepared from 50-70% of enzymolysis fish protein stock solution and other components; the stock solution is prepared by pulping aquatic product processing leftovers, adjusting the pH value to 6.5-7.5, adding specific compound protease for enzymolysis, and performing enzyme deactivation and centrifugation; the macroelement liquid contains nitrate nitrogen and ammonium nitrogen (2: 1-4: 1), and the microelement chelating agent is prepared by reacting a stock solution small molecule peptide with a specific salt. The fish protein enzymolysis stock solution is prepared from aquatic product processing leftovers, high-proportion small molecule peptide and amino acid are obtained through specific enzymolysis and other processes, and by matching with high-content macroelements in a specific nitrogen form, nutrition can be rapidly supplemented, and crop photosynthesis and dry matter accumulation can be improved; and moreover, all the components cooperate to regulate the growth of the crops and improve the stress resistance, and the surfactant also improves the utilization rate of the fertilizer and reduces waste.

The invention discloses compound enzyme nanogel as well as a preparation method and application thereof. The compound enzyme nanogel is prepared from the following components in parts by weight: 12 to 1310 parts of compound protease; 5-210 parts of a monomer polymer material; 12 to 120 parts of an adhesive; and 12-120 parts of a cross-linking agent. The compound enzyme nanogel disclosed by the invention improves the loading capacity of protein, and is high in stability and good in safety; in addition, due to the stability of the nanogel structure, the protease has a relatively long half-life period, an enzyme cascade effect can be continuously and effectively carried out, and meanwhile, the administration concentration and the administration frequency are reduced.

The invention relates to the field of vegetable protein gel products, in particular to rapeseed protein-soybean protein isolate composite gel and a preparation method thereof. The preparation method of the composite gel comprises the following steps: mixing rapeseed protein and soybean protein isolate, adjusting the pH value to 6-8, heating in a water bath at 80-100 DEG C, and immediately cooling to obtain the composite gel; the preparation method of the rapeseed protein comprises the following steps: crushing degreased rapeseed meal, dissolving the crushed degreased rapeseed meal in a sodium chloride solution according to a solid-to-liquid ratio of 1: (8-12), adjusting the pH value to 7.5-8.5, carrying out a reaction for 30-60 min, and carrying out centrifugation, membrane filtration, secondary centrifugation, dialysis and drying on the product to obtain the rapeseed protein. The hardness, elasticity, cohesiveness and chewiness of the compound protein gel prepared on the basis of the rapeseed protein are superior to those of soybean protein isolate gel. And the viscosity and modulus of the compound protein gel are superior to those of SP protein, and the compound protein gel has better external force resistance.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention discloses natto oligopeptide capable of relieving formation of arterial plaques and a preparation method and application thereof, belongs to the field of active peptide preparation, aims to solve the problem of obtaining natto or oligopeptide thereof capable of relieving formation of atherosclerotic plaques, and is characterized in that soybeans are soaked, sterilized, inoculated with compound bacteria, fermented at constant temperature and subjected to after-ripening to obtain natto, the volume ratio of the bacillus natto to the lactobacillus plantarum to the bifidobacterium bifidum in the compound bacteria is 1: (0.3-0.5): (0.1-0.3); compound protease is added into the aqueous solution of the freeze-dried natto powder for hydrolysis, centrifugation and filtration, components below 30000 Da are intercepted to obtain the natto oligopeptide, the ratio of chymotrypsin to papain in the compound protease is (1-3): 1, and the natto oligopeptide has the effects that the content of nattokinase in natto is higher, the protein level is also improved, and the natto oligopeptide can be used for preparing the natto oligopeptide. The nattokinase activity is well reserved in hydrolysis, the effect of promoting macrophage autophagy is achieved, and atherosclerotic plaque formation can be relieved.