248 results about "Complex protein" patented technology

The present invention provides methods of screening for compounds that interfere with the interaction between ERG. ETV1, ETV4 or ETV5 and mSWI / SNF (BAF) chromatin remodeling complex proteins. Included are methods of screening for compounds that interfere with the interaction between ERG, ETV1, ETV4 or ETV5 and BAF155. Methods of treating prostate cancer with compounds that interfere with the interaction between ERG, ETV1, ETV4 or ETV5 and mSWI / SNF (BAF) chromatin remodeling complex proteins are also provided.

The invention provides an intelligent response type medical liquid application and a preparation method thereof, and relates to the technical field of medical dressings. The liquid application is composed of a basic repairing component, a natural antibacterial component, a plant active component, an intelligent carrier system and auxiliary materials. Accurate release of active ingredients is realized by adopting a pH / temperature double-response intelligent delivery system, and the transdermal absorption rate is remarkably improved; wound healing is accelerated through the synergistic effect of the compound protein and the plant extract; active ingredients are effectively reserved through a multi-mode sterilization process. The liquid application disclosed by the invention has the characteristics of strong antibacterial property, good environmental adaptability, excellent healing effect and the like.

The invention relates to the field of biological medicine, and provides a preparation method of a fermentation product containing giant salamander peptide, which comprises the steps of stepped enzymolysis pretreatment, staged inoculated fermentation, synergistic enzymolysis and molecular weight grading purification. The method comprises the following steps: firstly, carrying out staged enzymolysis on giant salamander tissues by utilizing pepsin, trypsin and flavourzyme to obtain a primary peptide solution; then, bacillus subtilis, lactobacillus plantarum and bifidobacterium are adopted for directional fermentation, and flora metabolism is optimized through dissolved oxygen control and pH automatic regulation. Then, compound protease is added for secondary enzymolysis, so that the yield of the small molecule peptide is increased. And finally, carrying out ultrafiltration and nanofiltration graded purification, vacuum concentration, freeze drying and gamma-ray sterilization to obtain the micromolecular giant salamander peptide. The molecular weight of the obtained product is mainly distributed within 0.5-5 kDa (lt), and the proportion of 3 kDa is larger than or equal to 80%), the DPPH free radical scavenging rate is larger than or equal to 85%, the ABTS < + > scavenging rate is larger than or equal to 80%, and the obvious anti-oxidation and auxiliary liver protection effects are achieved.

The invention discloses a spirulina platense skin extract as well as a preparation method and application thereof, and relates to the technical field of biomedicine. The obscured spirulina skin extract is rich in various active ingredients including collagen family members, viscose protein, C1Q complement protein, fibronectin and the like, and the ingredients complement and promote one another, so that the extract has remarkable effects in the aspects of skin aging resistance, skin injury repair and the like; therefore, the method has a wide application prospect in medicine research and development, cosmetics and functional foods. According to the extraction method of the skin protein of the obtuse spirulina, the sample is subjected to rapid freeze thawing treatment, and the steps of mechanical crushing, ultrasonic-assisted separation and multiple filtration are combined, so that the influence on the activity of the product is avoided, and various compound proteins can be obtained; the pH value of a buffer solution is adjusted, the property of the extract is further optimized, and the extract is more suitable for human skin, is sterile and can be directly used as a product raw material, so that large-scale production is realized, and industrial application of the obtuse spirulina skin extract is promoted.

The invention provides a fructus cannabis protein peptide capable of reducing blood sugar and regulating glycometabolism and a preparation method and application thereof, and belongs to the technical field of functional active peptides, and the preparation method comprises the following steps: performing low-temperature supersonic fluid crushing on fructus cannabis, performing enzymolysis pretreatment on the crushed fructus cannabis, adding compound protease for enzymolysis, adding PNGaseF enzyme for further enzymolysis, and performing freeze drying to obtain the fructus cannabis protein peptide capable of reducing blood sugar and regulating glycometabolism. And carrying out membrane ultrafiltration interception and enrichment on the enzymolysis supernatant, carrying out tangential flow filtration, concentrating, drying and sterilizing to obtain the fructus cannabis protein peptide. Tests prove that the novel fructus cannabis protein peptide has the following remarkable effects that the fasting blood glucose level can be remarkably reduced, and uncomfortable symptoms such as thirst, polydipsia, diuresis and weakness caused by hyperglycemia are relieved; the symptoms of glucose intolerance and insulin resistance caused by hyperglycemia can be obviously relieved, and the onset risk of type 2 diabetes mellitus is reduced; the level of fasting serum insulin and glycosylated hemoglobin can be obviously reduced, and the level of serum GLP-1 can be improved.

The invention discloses pericarpium citri reticulatae compound protein powder as well as a preparation method and application thereof. The protein powder takes pericarpium citri reticulatae extract microcapsules, seville orange flower extract microcapsules, soybean protein isolate powder, polydextrose, resistant dextrin, soybean peptide powder, tremella powder and the like as main components, and further comprises edible salt, edible essence, a nutrition enhancer, a sweetening agent and an anti-caking agent. The preparation method comprises the steps of raw material weighing, premixing, high-speed shearing and mixing and the like. Active components of the dried orange peel and seville orange flower extract are extracted through an ultrasonic-assisted ethanol extraction method, and sodium alginate and chitosan are adopted for microencapsulation treatment, so that the stability and solubility of the dried orange peel and seville orange flower extract are improved. The compound protein powder disclosed by the invention has the functions of maintaining muscle mass and relieving symptoms such as nausea and abdominal distension in a weight loss process, has good storage stability and safety, and is suitable for being taken for a long time.

The invention discloses a preparation method and application of squid collagen peptide with beauty maintaining and young keeping effects, and belongs to the technical field of marine food processing. The collagen is obtained through enzymolysis hydrolysis, the structure of the collagen is kept, damage to amino acid is avoided, treatment and use of acid, alkali and organic solvents are reduced in the preparation process, and equipment loss and environmental pollution are avoided. The second enzymolysis is performed by adopting the synergistic effect of the compound protease, the low molecular weight, degreasing and bitterness removal of the peptide powder can be realized, and the obtained peptide powder is low in molecular weight, good in flavor, free of peculiar smell and suitable for being eaten, digested and absorbed by human bodies. Experiments prove that the squid collagen peptide prepared by the preparation method disclosed by the invention has relatively strong oxidation resistance and has remarkable effects in the aspects of whitening, wrinkle resistance, oxidation resistance and the like.

The invention relates to the technical field of medical care, and particularly discloses a preparation method and application of sea cucumber peptide. The preparation method of the sea cucumber peptide provided by the invention comprises the following steps: heating sea cucumber primary pulp for denaturation, and then adding compound protease for enzymolysis treatment to obtain sea cucumber enzymatic hydrolysate; the addition amount of the compound protease is 0.1-0.15 mg / mL, and the compound protease is prepared from papain, ficin and trypsin according to the weight ratio of 1: (0.5-1.5): (2-3); adding activated carbon and diatomite into the sea cucumber enzymatic hydrolysate, and performing enzyme deactivation, filtration and concentration treatment to obtain sea cucumber peptide; the invention also provides application of the sea cucumber peptide obtained by the preparation method in medicines and foods. According to the preparation method of the sea cucumber peptide, the sea cucumber peptide with small molecular weight and good uniformity can be obtained, and the prepared sea cucumber peptide tablet candy has the effects of relieving physical fatigue, resisting aging, resisting oxidation and the like.

The invention belongs to the technical field of bioengineering, and discloses a sunflower seed peptide for regulating metabolism of brain cells, the sunflower seed peptide has a biological sequence of Arg His Ala Lys Thr Pro Ser Asn Lys, and a preparation method of the sunflower seed peptide for regulating metabolism of brain cells comprises the following specific steps: S1, raw material pretreatment; s2, peeling and crushing; s3, protein extraction; s4, performing enzymolysis treatment; s5, performing enzyme deactivation treatment; s6, filtering and purifying; s7, concentrating and drying; and S8, quality detection and packaging. The sunflower seed protein is directionally decomposed into the active peptide fragment by precisely regulating and controlling compound protease and enzymolysis conditions, the active peptide fragment can be compatible with a brain cell specific receptor and penetrate through a blood brain barrier, and cell experiments and animal models prove that the peptide fragment can activate a mitochondrial respiratory chain complex, so that the mitochondrial membrane potential is increased by more than 30%, and the mitochondrial membrane potential is increased by more than 30%. Meanwhile, the activity of neurotransmitter synthetase can be enhanced, the brain health is protected, and tests further prove that the sunflower seed peptide also has the function of regulating the metabolism of brain cells.

The invention provides collagen tripeptide liquid with moisturizing and anti-photoaging effects and a preparation method thereof, and relates to the technical field of bioactive peptides.The preparation method comprises the steps that pretreatment and first-time enzymolysis are conducted, specifically, pretreated fish skin and water are mixed, the pH value is adjusted, compound protease is added for enzymolysis, and first enzymatic hydrolysate is obtained; second-time enzymolysis: adjusting the pH value of the first enzymatic hydrolysate to 6.0-7.5, adding neutral protease for enzymolysis, and then adding aminopeptidase for enzymolysis for 20-40 minutes to obtain second enzymatic hydrolysate; and performing enzyme deactivation, decoloration, separation and purification to obtain collagen tripeptide liquid. Wherein the content of collagen tripeptide, the content of glycine-proline-hydroxyproline and the content of hydroxyproline are high, and good moisturizing and anti-photoaging effects are achieved.

The invention discloses oyster-soybean compound protein gel constructed based on low-temperature synergy as well as a preparation method and application of the oyster-soybean compound protein gel, and belongs to the technical field of functional foods. The composite gel is formed by oyster protein and soybean protein isolate through low-temperature stabilization, medium-temperature construction and thermal induction treatment. The preparation method comprises the following steps: preparing an oyster protein suspension; preparing a soybean protein isolate suspension; the oyster protein suspension and the soybean protein isolate suspension are mixed, stirred and left to stand, a gel structure is preliminarily constructed and then subjected to thermal induction curing treatment, and after the thermal induction curing treatment is finished, the oyster-soybean compound protein gel is formed through storage at low temperature and can be used in the field of food processing. The oyster protein and the soybean protein isolate are combined, so that the composite gel has excellent gel strength, smooth texture and long-acting storage stability, no organic chemical reagent or food additive is used in the preparation process, the formula safety is ensured, the process is simple and convenient, and the composite gel shows huge application value and industrialization prospect.

The invention discloses a polypeptide fertilizer beneficial to cold resistance of crops and a preparation method thereof, and belongs to the technical field of agricultural fertilizers, the fertilizer is prepared by taking livestock and poultry blood (preferably pig blood) as a raw material, preparing high iron protein peptide through a compound protease hydrolysis process, and matching with a trace element compound, humic acid and a carrier. The preparation process comprises the steps of enzymolysis preparation of the ferritin peptide, fertilizer mixing, granulation, drying and the like. The polypeptide fertilizer disclosed by the invention takes the high-iron protein peptide as a core, the contained small-molecular amino acid polypeptide can enhance the antioxidant enzyme activity of crops at low temperature and reduce malonaldehyde accumulation, the porphyrin iron can promote chlorophyll synthesis and energy metabolism, and the high-iron protein peptide and the porphyrin iron cooperate to remarkably improve the cold resistance of the crops. Meanwhile, by cooperating with trace elements and humic acid, the cold resistance is enhanced, nutrient absorption of crops is promoted, the yield and quality are improved, multiple effects of resisting cold, improving quality, increasing yield and the like are achieved, the raw materials are wide in source, the cost is low, the preparation process is simple, and large-scale production and application are facilitated.

The invention relates to the technical field of agricultural fertilizers, in particular to an enzymolysis fish protein type amino acid-containing water-soluble fertilizer and a preparation method thereof, the fertilizer is prepared from 50-70% of enzymolysis fish protein stock solution and other components; the stock solution is prepared by pulping aquatic product processing leftovers, adjusting the pH value to 6.5-7.5, adding specific compound protease for enzymolysis, and performing enzyme deactivation and centrifugation; the macroelement liquid contains nitrate nitrogen and ammonium nitrogen (2: 1-4: 1), and the microelement chelating agent is prepared by reacting a stock solution small molecule peptide with a specific salt. The fish protein enzymolysis stock solution is prepared from aquatic product processing leftovers, high-proportion small molecule peptide and amino acid are obtained through specific enzymolysis and other processes, and by matching with high-content macroelements in a specific nitrogen form, nutrition can be rapidly supplemented, and crop photosynthesis and dry matter accumulation can be improved; and moreover, all the components cooperate to regulate the growth of the crops and improve the stress resistance, and the surfactant also improves the utilization rate of the fertilizer and reduces waste.

The invention relates to the field of biological product preparation, in particular to a preparation method of high-activity oyster-derived small molecule peptide. The method comprises the following steps: selecting fresh meat of pacific oysters or ostrea rivularis as a raw material, degreasing, adding water, crushing, filtering, sequentially carrying out compound protease and neutral protease hydrolysis, implanting a sensor and a probe in hydrolysis, monitoring in real time, and dynamically adjusting reaction conditions; adding a targeting microcapsule wrapping an ascorbic acid glutathione composite protective agent, and releasing the protective agent when a target peptide signal is detected; separating and concentrating through a gradient membrane, and purifying the target peptide by using a molecular imprinting chromatographic column; carrying out alkaline hydrolysis on enzymatic hydrolysis residues to prepare chitosan for cyclic utilization, carrying out enzymatic hydrolysis on residual residues to generate collagen peptide, treating and recycling wastewater, and recovering protease. According to the method, the technical effects that the high-activity and high-purity target peptide is prepared, the resource utilization rate is increased, the production cost is reduced, the target peptide has good oxidation resistance and biological activity, the intestinal enzyme degradation rate is reduced after glycosylation modification, and the blood half-life period is prolonged are achieved.

The invention discloses a protein multi-modal joint modeling method and system based on cross-modal alignment, and aims to realize unified representation and processing of protein text description, sequence information and structural features so as to improve the accuracy and generalization ability of complex protein analysis and prediction tasks and open research tasks. The method comprises the following steps: firstly collecting and preprocessing protein multi-modal data to generate standardized representation, extracting features by using a geometric graph neural network and a protein sequence model, aligning the features through a projection module, transmitting the features to a large language model, performing projection fusion to generate uniform invariant features, and finally finishing a protein analysis and prediction task in combination with the invariant features. According to the method, multi-modal information of the protein can be effectively integrated, efficient alignment and fusion of cross-modal features are achieved, the ability to understand and predict complex characteristics of the protein is improved, and powerful support is provided for precise bioinformatics analysis and biological medicine research.

The invention discloses an easily absorbable organic calcium powder containing mineral elements and a preparation process thereof, and the raw materials comprise, by weight, 50-70 parts of an organic calcium source, 20-30 parts of a plant protein source, 5-10 parts of a polybasic amino acid chelate, 0.01-0.03 part of vitamin D3, 1-3 parts of trace mineral elements, 0.5-1.5 parts of compound protease, 0.1-0.3 part of a food-grade surfactant, and 100-150 parts of deionized water. The invention relates to the technical field of nutritional supplements. According to the mineral element-containing easy-to-absorb organic calcium powder and the preparation process, vegetable protein is converted into amino acid through biological enzymolysis, the amino acid and calcium carbonate are subjected to a chelation reaction to generate amino acid chelated calcium, and compared with traditional organic calcium, chelated calcium is more easily absorbed by human intestinal tracts; meanwhile, calcium, magnesium and phosphorus are synergistically matched, magnesium can activate vitamin D3, the vitamin D3 further promotes absorption of calcium, phosphorus and calcium form a reasonable proportion, deposition of calcium in bones is enhanced, and calcium ions can be absorbed in intestinal tracts without depending on gastric acid by adopting a bicyclic stable chelating structure.

The present invention discloses a protein multimodal joint modeling method and system based on cross-modal alignment, which aims to achieve unified representation and processing of protein text descriptions, sequence information and structural features, so as to improve the accuracy and generalization ability of complex protein analysis and prediction tasks and open research tasks. The present invention first collects and pre-processes protein multimodal data to generate a standardized representation, uses a geometric graph neural network and a protein sequence model to extract features, passes them to a large language model after alignment by a projection module, and then projects and fuses them to generate unified invariant features, and finally combines equivariant features to complete the protein analysis and prediction task. The present invention can effectively integrate the multimodal information of proteins, achieve efficient alignment and fusion of cross-modal features, improve the understanding and prediction capabilities of complex protein properties, and provide strong support for precise bioinformatics analysis and biomedical research.

The present invention discloses an axolotl skin extract, its preparation method and applications, relating to the technical field of biomedicine. The axolotl skin extract is rich in various active ingredients, including members of the collagen family, fibronectin, C1Q complement protein, fibronectin, etc. These ingredients complement and promote each other, making the extract have remarkable effects in aspects such as skin anti-aging and skin injury repair, and thus having broad application prospects in drug R & D, cosmetics and functional foods. The method for extracting axolotl skin protein, through rapid freeze-thaw treatment of samples, combined with mechanical crushing, ultrasonic-assisted separation and multiple filtration steps, avoids the influence on the activity of the product, and can obtain a variety of composite proteins; adjusting the pH value of the buffer solution further optimizes the properties of the extract, making it more suitable for human skin, and being sterile and directly usable as the raw material of the product, realizing large-scale production and promoting the industrial application of axolotl skin extract.

The invention discloses a composite nut polypeptide solid beverage and a preparation method thereof. The health-care food is prepared from the following components in parts by weight: 20 to 40 parts of walnuts, 15 to 30 parts of cashew nuts, 10 to 25 parts of almonds, 12 to 28 parts of almonds, 8 to 20 parts of hazelnuts, 0.3 to 0.8 part of compound protease, 0.05 to 0.15 part of beta-glucanase, 10 to 20 parts of isomaltooligosacharide, 5 to 15 parts of resistant dextrin, 8 to 18 parts of maltodextrin, 0.1 to 0.3 part of silicon dioxide, 0.05 to 0.15 part of vitamin C, 0.02 to 0.08 part of gamma-aminobutyric acid, 0.03 to 0.1 part of taurine and 2 to 5 parts of natural strawberry powder. And 0.05 to 0.15 part of natural vanilla essence. The invention discloses and provides a composite nut polypeptide solid beverage and a preparation method thereof, a composite enzymolysis technology is adopted, nut protein is hydrolyzed into small-molecule polypeptides with the average molecular weight lower than 1000Da, and the polypeptides have high biological activity and bioavailability.

The invention discloses a processing method of sheepskin food, and belongs to the technical field of food processing. In order to solve the problems that in the existing sheepskin processing, the fishy smell is not thoroughly removed, calcium salt permeation is not uniform due to compact tissues, the taste difference of products is large due to hard outside and soft inside, and the like, the method comprises the following steps: unhairing, washing and dicing fresh sheepskin; putting the skin blocks into a reaction solution containing compound protease and flavourzyme for enzymolysis; transferring the skin blocks subjected to enzymolysis into an aqueous solution containing edible calcium salt and organic acid to be soaked; and finally marinating, cooling, shaping and packaging the skin blocks. According to the method, the fishy smell is effectively removed through composite enzymolysis, tissues are moderately softened, then calcium salt and organic acid coupling embrittlement treatment is performed, a uniform gel network is formed through ionic crosslinking, and the texture of skin blocks is remarkably improved. The method is easy and convenient to operate and mild in condition, and the prepared sheepskin food is low in fishy smell, crisp, tender and uniform in taste, sufficient in flavor permeation and suitable for high-value utilization and industrial production of sheepskin.

The method comprises the following steps: drying and crushing shrimp heads and shrimp shells, adding water according to a ratio of 1: 3, adding 0.5%-0.7% of compound protease, and carrying out enzymolysis at 50-55 DEG C for 2-3 hours; after enzyme deactivation, adding 7-10% of brown sugar to adjust the pH value to 6.0-6.5, and sterilizing; the method comprises the following steps: inoculating pineapple endophytic lactic acid bacteria (CGMCC 25352), hansenula polymorpha (CGMCC 25353) and aspergillus oryzae, mixing the three strains according to the viable count of 1: 1: 1 (the total inoculum size is 3-4.5%, and the CFU / g is 10-10), and fermenting for 46-48 hours at the temperature of 30-32 DEG C in a dark place; and filtering and storing at-80 DEG C. The product is free of salt addition, the fermentation period is less than or equal to 48 hours, the amino acid nitrogen content is greater than or equal to 0.51 g / 100g, the total content of lactic acid (6.88 mg / g), succinic acid (3.73 mg / g) and free amino acid is greater than or equal to 313mg / 100g, the volatile amino salt content is less than or equal to 38.72 mg / 100mL, and the product has salty and fresh flavor and orange-yellow clarified appearance. The problems of high salt, long period and heavy fishy smell of the traditional shrimp soy sauce are solved.

The invention discloses a multi-source composite peptide instant solid beverage and an enzymolysis preparation method thereof, and belongs to the technical field of functional food processing.The beverage is prepared by taking animal, plant, microorganism, insect and algae five-element composite protein sources as raw materials, conducting enzymolysis and then compounding with functional auxiliary materials, a flavoring agent and a stabilizer. Wherein the active peptide fragment is high in proportion and is modified by functional groups. The preparation method comprises the following steps: crushing the multi-element protein, carrying out ultrasonic pretreatment, carrying out enzymolysis by adopting a compound enzyme system containing multiple proteases, carrying out enzyme deactivation and nanofiltration graded purification, and carrying out supercritical COspray drying or freeze drying to obtain the solid beverage. The problems that a traditional peptide beverage is single in protein source, low in biological activity, poor in instant solubility and the like are solved, the obtained product is comprehensive in amino acid composition and has the characteristics of rapid cold water instant dissolution, long-term stability and non-layering, a synergistic effect is formed among functional components, the absorption efficiency and physiological functions such as immune regulation and oxidation resistance are remarkably improved, and the beverage is suitable for large-scale production. Wide industrial application prospects are realized.

The invention discloses a special oil nutrition composition rich in flaxseed polysaccharide and compound protein and a preparation method and application thereof, and belongs to the technical field of functional foods.The nutrition composition is prepared from wall materials and core materials, and the wall materials comprise, by mass, 1-20% of flaxseed polysaccharide, 1-20% of compound protein, 1-20% of compound protein and the balance of water. The core material comprises the following materials in percentage by weight: 45%-55% of vegetable oil, 0.2%-2% of an emulsifier and 0.01%-0.2% of a fat-soluble antioxidant, and the core material comprises the following materials in percentage by weight: 1%-15% of whey protein powder, 1%-15% of soybean protein isolate, 15%-45% of a micromolecular filler and 0.05%-0.2% of a water-soluble antioxidant. The preparation method comprises the following steps: preparing a mixed material from the core material mixture and the wall material mixture; the nutritional composition disclosed by the invention has the advantages that the synergistic embedding effect of the nutritional composition is remarkable, the oxidation stability is excellent, the nutrition synergistic interaction is realized, and the processing applicability is high.

The invention relates to a novel amino acid foliar fertilizer and a preparation method thereof, and belongs to the technical field of agricultural fertilizers. The method aims at solving the technical problems that in the prior art, the amino acid conversion rate is low, product stability is poor, and severe conditions such as strong acid and strong alkali are possibly used. The method comprises the following steps: mixing soybean meal, fish meal and saccharomyces cerevisiae thalli to form a compound protein raw material, performing two-stage crushing and degreasing, performing step-by-step enzymolysis by adopting a compound enzyme preparation compounded by alkaline protease and flavourzyme, performing enzyme deactivation, and performing ultrafiltration by adopting a ceramic membrane to obtain purified amino acid hydrolysate, and mixing the hydrolysate with a composite trace element mixed solution formed by EDTA-Fe, EDTA-Zn, boric acid, ammonium molybdate and citric acid, adding humic acid, a seaweed extract and an organic silicon penetrant, and carrying out high-speed shearing, filtering and vacuum degassing to finally obtain the product. The method is mainly used for producing the amino acid foliar fertilizer which is high in free amino acid content, good in stability and easy to absorb by plants.

The invention relates to a pharmaceutical composition for repairing oral gingivitis and a preparation method thereof, and belongs to the technical field of biological medicine, the pharmaceutical composition comprises a lactobacillus salivarius fermentation extract, a herba violae extract, golden cicada nymph extract peptide, guava polyphenol and resveratrol; the lactobacillus salivarius fermentation extract is obtained by carrying out aerobic fermentation and anaerobic fermentation on lactobacillus salivarius to obtain a fermentation product, and purifying and extracting the fermentation product through an XAD7HP macroporous adsorption resin column; the philippine violet herb extract is obtained by performing enzymolysis on whole philippine violet herb through pectinase and cellulase and then performing enzymolysis through compound protease to obtain zymolyte, and purifying and extracting the zymolyte through a compound adsorption column. The golden cicada nymph extracted peptide is obtained by purifying and extracting 5kDa zymolyte obtained by performing step-by-step enzymolysis on golden cicada nymphs through papain and trypsin through a Q agarose gel FF adsorption column. All the components are matched for use according to a certain proportion, so that the antibacterial coating is capable of precisely resisting bacteria, has efficient anti-inflammatory and anti-oxidation properties, and is safe and reliable.

The invention relates to a preparation method of compound protein composite gel. The preparation method comprises the following steps: step 1, mixing ball-milled pea starch with water to prepare a starch emulsion; treating to gelatinize the ball-milled pea starch; taking out and cooling to room temperature to obtain pea starch gel, aging and then crushing to obtain pea resistant starch; step 2, dissolving whey protein isolate powder in water to prepare a protein gel stock solution; adding the pea resistant starch according to the proportion of 0.25-1w / v% of the protein gel stock solution; after mixing, continuously stirring at room temperature for 1-3 hours; storing overnight under the condition of a refrigerator at 4 DEG C to ensure that the starch is dissolved with the protein to obtain a gel stock solution for later use; and step 3, taking out the gel stock solution, placing the gel stock solution in a water bath kettle with the temperature of 90-98 DEG C for heat treatment for 20-40 minutes, taking out the gel stock solution, placing the gel stock solution in an ice-water bath for cooling for at least 10 minutes to quickly form gel, and then placing the gel in a refrigerator with the temperature of 4 DEG C for storage. The pea resistant starch is added into the whey protein isolate, so that the synergistic interaction effect on the compound protein gel can also be realized.

The invention discloses a preparation method and application of enzymolysis royal jelly capable of whitening skin, fading spots and resisting wrinkles, and belongs to the technical field of enzymolysis of royal jelly. The technical problem to be solved is that the existing royal jelly zymolyte is lack of research having the effects of whitening, fading spots and resisting wrinkles. The key point of the technical scheme is that the preparation method of the enzymolysis royal jelly comprises the following steps: (1) mixing and homogenizing royal jelly and water, and adjusting the pH value to obtain a royal jelly solution; (2) adding compound protease into the royal jelly solution for enzymolysis, and performing enzyme deactivation and solid-liquid separation to obtain royal jelly enzymatic hydrolysate; the compound protease in the step (2) is fig protease, chymotrypsin and flavourzyme.

The invention belongs to the field of feed protein processing, and particularly relates to a method for improving the bioavailability of animal feed protein through chemical enzymolysis. The method comprises the following steps: mixing a compound protein raw material with water to form a suspension; sodium tripolyphosphate is added for phosphorylation pretreatment, so that protein is subjected to specific modification; after the pH is adjusted to be acidic, pepsase and acid phosphatase are added at the same time for an enzymolysis reaction; and after the reaction is finished, performing enzyme deactivation and drying to obtain the modified feed protein. According to the method, a specific enzyme cutting site is created on a protein molecule through chemical phosphorylation, then directional hydrolysis is achieved through efficient recognition of pepsin on the site, and meanwhile dynamic regulation and control of the hydrolysis process are achieved by means of the dephosphorylation effect of acid phosphatase. The obtained product has the outstanding advantages of high content of small peptides, good digestive absorbability, low bitterness, strong biological activity and the like, and the bioavailability of feed protein is remarkably improved.

The invention discloses goat milk with hypoglycemic activity and a preparation method thereof, and belongs to the technical field of enzyme engineering. The goat milk is used as a raw material, enzymolysis goat milk with alpha-G inhibitory activity is obtained through compound protease hydrolysis, it is found that the enzymolysis goat milk has hypoglycemic activity, and the goat milk powder with the hypoglycemic activity is further prepared through spray drying. The alpha-G inhibitory peptides in the enzymolysis goat milk are separated and identified, 20 alpha-G inhibitory peptides are found from components with the highest alpha-G inhibition rate, the molecular weight of the alpha-G inhibitory peptides is 377-962 Da, and the alpha-G inhibitory peptides are mainly derived from beta-casein, alpha S1-casein, alpha S2-casein, kappa-casein, beta-lactoglobulin, lactoglobulin and osteopontin of the goat milk. The alpha-G inhibitory peptide is prepared by hydrolyzing the goat milk through the compound enzyme, the amino acid sequence of the alpha-G inhibitory peptide is separated and identified, the goat milk with hypoglycemic activity is developed, and theoretical basis and technical support are provided for subsequent development of hypoglycemic dairy products.

The present invention discloses a salty and fresh bifunctional flavor peptide and its preparation method and application, and relates to the field of biotechnology. The amino acid sequence of the salty and fresh bifunctional flavor peptide is shown in any one of SEQ ID NO.1-4. The present invention adopts a composite protease enzymatic hydrolysis method to prepare an enzymatic hydrolyzate rich in salty and fresh bifunctional flavor peptides, and uses peptide omics and molecular docking technology to identify and screen out multiple salty and fresh bifunctional flavor peptides, while evaluating and predicting the transmembrane domains of taste receptors, and analyzing the chemical bonds and docking sites of the salty and fresh bifunctional flavor peptides docking with taste receptors, summarizing the key chemical bonds and binding sites of the interaction between the salty and fresh bifunctional flavor peptides and salty receptors, and evaluating their salty and umami intensity. The salty and fresh bifunctional flavor peptides screened by the present invention have strong saltiness and umami, can be used as a substitute for sodium salt, and are used to prepare low-salt and low-sodium foods. The present invention also provides a certain reference for quickly and easily obtaining salty and fresh bifunctional flavor peptides.