479 results about "Fish oil" patented technology

The invention relates to the technical field of ceramic materials, in particular to a method for reducing the surface roughness of a silicon nitride instant-fired sheet, which comprises the following steps: S1, mixing alpha-silicon nitride, beta-silicon nitride, absolute ethyl alcohol and herring fish oil, grinding and dispersing to obtain basic mixed powder; s2, mixing, grinding and dispersing the filling mixed powder, absolute ethyl alcohol and herring fish oil, then carrying out filter pressing and drying, calcining and regrinding to obtain ground filling mixed powder; s3, mixing and ball-milling the basic mixed powder, the ground filling mixed powder, a solvent, an adhesive, a plasticizer and a dispersing agent to obtain slurry; s4, casting the slurry into a film; s5, attaching the thin film to the surface of the silicon nitride green body, and pressing the silicon nitride green body into a whole piece through an isostatic press to obtain a pressed green body; s6, a boron nitride isolation layer is printed on the surface of the pressed green body, and the green body with the isolation layer is obtained; and S7, stacking the green bodies with the isolating layers, and then carrying out adhesive removal and sintering reaction to obtain the silicon nitride ceramic chip.

The invention discloses an oral instant film suitable for pet nutrition intervention and a preparation method of the oral instant film, and belongs to the technical field of pet oral preparations. The instant film adopts a sandwich type multi-layer structural design, sequentially comprises a first food calling layer, a core layer and a second food calling layer, can be selectively provided with a functional layer and an interface middle layer, and constructs a staggered hot-pressing laminated structure, so that the structural stability and the function delivery efficiency of a film body are remarkably improved. The core layer contains multiple composite active ingredients such as L-carnitine, coenzyme Q10, beta-glucan, glucosamine sulfate and the like, chitosan-inulin-polyglutamic acid or starch is adopted for embedding to form a first microcapsule and a second microcapsule, and intelligent delivery of temperature / pH response and gastrointestinal controlled release is achieved. The food calling layer is composed of chicken powder, fish oil powder, fructo-oligosaccharide and the like, and the feeding compliance of pets is improved.

The invention provides a water-blocking microcapsule, a fish oil composite hair beautifying ointment and a preparation method, and relates to the technical field of animal health products. The water-blocking microcapsule is a solid particle formed by a core material and a wall material coating the core material; the core material is fish oil emulsion subjected to bile salt emulsification treatment; the wall material sequentially comprises an inner-layer wall material and an outer-layer wall material from inside to outside; the inner-layer wall material is a wall material formed by solidifying alginate; and the outer-layer wall material is a water-blocking wall material which is arranged outside the inner-layer wall material and is made of nanocrystalline cellulose. The water-blocking microcapsule provided by the invention realizes multiple beneficial effects of efficient digestion and absorption of fish oil, improvement of palatability, enhancement of oxidation resistance and stability, fixed-point release of intestinal tracts and the like, provides an innovative technical scheme for development of animal health products, and has wide application prospects and market values.

The invention provides a method for separating EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) through supercritical fluid chromatography, and relates to the technical field of separation and purification. The invention provides a method for separating EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) by supercritical fluid chromatography, which comprises the following steps: by taking supercritical carbon dioxide and an organic reagent as mobile phases, and by utilizing the specific mobile phase property of supercritical CO2 after being mixed with the organic reagent (saturated aliphatic hydrocarbon) and matching with a normal-phase chromatographic column, separating the EPA from the DHA by supercritical fluid chromatography. A specific stationary phase (one or more of a silica gel surface bonded propylurea stationary phase, a silica gel surface bonded phenyl stationary phase and a silica gel surface bonded imidazolyl stationary phase) is adopted, effective separation and preparation of the EPA and the DHA in the fish oil can be achieved, the EPA and the DHA in the fish oil can be effectively separated within 50 min, the separation speed is high, the separation effect is good, and the method is suitable for industrial production. The purity of the obtained EPA and DHA is 98% or above, the purity of the EPA and the DHA is high, and the EPA and the DHA have good application prospects in the fields of medicine production and food and health care products.

The invention belongs to the field of feed, and discloses balance oil which comprises the following components in percentage by weight: 15-20% of fish oil, 20-30% of soybean oil, 5-10% of linseed oil, 20-30% of lysophospholipid, 5-10% of coconut oil, 15-20% of corn oil and 1-5% of red palm oil. The balance oil is applied to pregnant sows, and experimental verification shows that the balance oil can effectively improve the reproductive performance of the sows and the growth performance of piglets. Meanwhile, the invention further discloses balancing grease powder and application thereof.

The invention relates to the technical field of sample preparation equipment, in particular to deep sea fish oil micro-capsule determination sample preparation equipment which comprises a motor base, a linkage case is fixedly connected to the upper surface of the motor base, and a main motor is arranged in the motor base; a fish oil conveying box and a dispersing agent input box are fixedly connected to the upper portions of the two sides of the outer surface of the linkage machine box respectively, a stirring box is fixedly connected to a pipeline on the opposite sides of the fish oil conveying box and the dispersing agent input box, a rotating rod can move up and down through the action that a stirring assembly conducts sampling and pressing every time, the stirring uniformity is improved, and the stirring efficiency is improved. Single-batch multi-section sampling can improve the accuracy of the sample and reduce interference factors of various devices, meanwhile, the initial low speed can promote spreading of the micro-capsules, the rotation speed is increased to prevent oxidation of the dispersing agent and grease in the fish oil, and subsequent slow-down maintenance can facilitate fusion of the dispersing agent and avoid scattering of components in the deep sea fish oil through high-speed rotation; the observation is influenced.

The invention belongs to the technical field of aquaculture feed processing, and particularly relates to a processing technology of a sea cucumber intermediate culture feed. The sea cucumber intermediate breeding feed comprises fish meal, soybean protein isolate, seaweed meal, fermented soybean meal, multivitamins, a multivitamin premix and mineral salt. The processing technology comprises the following steps: firstly carrying out crushing pretreatment on the raw materials, then mixing the crushed raw materials, carrying out hardening and tempering, then carrying out twin-screw extrusion puffing to form carrier particles, carrying out low-temperature drying, then carrying out high-pressure atomization spraying technology to load the fish oil phospholipid complex, carrying out three-stage variable-temperature drying treatment, and finally carrying out cooling screening. The sea cucumber intermediate breeding feed processed by the method can be used for breeding in a transition stage from young sea cucumbers to young sea cucumbers, the survival rate of the young sea cucumbers in the breeding process is increased, and the sea cucumber intermediate breeding feed has the advantages of high nutrient retention rate and slow dissolution loss characteristic; in addition, the invention has the advantage of improving the order of micropores in the particles and the compactness of the surface layer so as to improve the physical stability of the sea cucumber intermediate culture feed.

The invention belongs to the field of microbial genetic engineering, and provides schizochytrium limacinum with high DHA content and low DPA content and application of the schizochytrium limacinum. According to the strain, an n3 front-end desaturase gene derived from crypthecodinium cohnii is integrated to a 18S rDNA site of schizochytrium limacinum ALS01, and omega-6 type DPA is converted into DHA by utilizing an extension-desaturation way. Under the optimized conditions of a 5L fermentation tank (the inoculation amount is 5-20%, the temperature is 25-28 DEG C, the ventilation ratio is 1.0-2.0 vvm, and fermentation is performed for 72-168 hours), the biomass is larger than or equal to 150g / L, the total grease is larger than or equal to 60%, the DHA total grease proportion is larger than or equal to 53%, the DPA total grease proportion is smaller than or equal to 2%, and the DHA yield is larger than or equal to 47.82 g / L. The invention solves the problems of unstable fish oil resource and insufficient DHA purity in the traditional DHA production, provides a new strain for the industrial production of high-purity DHA, and has important application value.

The invention relates to the technical field of pet food, in particular to flavoring agent powder for pet dogs and a preparation method of the flavoring agent powder. Comprising the following raw materials: 40 parts of beef liver, 10 parts of hairtail, 7-40 parts of ricefield eel, 1 part of protease, 0.5-1 part of lipase, 0.5-1 part of flavor enzyme, 5-10 parts of saccharides, 10 parts of fish oil, 5-10 parts of maltodextrin, 0.5-1 part of sodium hydroxide, 5 parts of phosphoric acid, 3 parts of amino acid, 2 parts of thiamine, 1 part of yeast extract and 0.5-1 part of lactoferrin. The dog feed can increase the appetite of dogs from the sense of smell, maintain the health balance of intestinal flora of dogs and improve the micro-ecological environment of intestinal tracts.

The invention relates to the technical field of bioengineering, and discloses Burkholderia lipase with T251S mutation and a recombinant expression vector and application thereof, threonine of the 251 amino acid sequence of LipA of wild Burkholderia lipase is mutated into serine. The enzyme activity of the mutant enzyme is remarkably improved by 23%, meanwhile, the hydrolysis capacity on ethyl ester type fish oil is remarkably improved, the selectivity on EPA is improved, and excellent EPA purification and enrichment effects are achieved. According to the mutant, the preparation cost is reduced, the hydrolysis capability and selectivity are improved, and the mutant has great popularization and application values.

The utility model discloses multistage separation and purification equipment for fish oil, which is applied to the technical field of fish oil processing and comprises a box body, a centrifugal cavity is arranged at the top of the box body, one side of the centrifugal cavity is rotatably connected with a connecting rod through a bearing, and a driving motor in bolted connection with the connecting rod is mounted on one side of the centrifugal cavity through a fixing piece. A fixed seat is bolted in the centrifugal cavity; the fixing seat is mounted in the centrifugal cavity, the centrifugal cylinder is mounted at the bottom of the fixing seat, when the driving motor drives the connecting rod to rotate, the centrifugal cylinder rotates under the action of the bevel gear and the bevel fluted disc, the purpose of centrifugal separation of fish oil containing impurities is achieved, and the cleaning brush mounted on the support is matched, so that the cleaning efficiency is improved. The centrifugal barrel can be continuously cleaned when the centrifugal barrel rotates, so that impurities are prevented from blocking holes of the centrifugal barrel when the centrifugal barrel performs centrifugal separation and purification on fish oil, and normal operation of centrifugal separation and purification work is guaranteed.

The invention relates to the technical field of computer vision, in particular to a method for quickly identifying apparent defects of fish oil soft capsules. The method comprises the following steps: acquiring a reference region in a fish oil soft capsule image to be identified; obtaining a seed point in the reference area and a corresponding growth threshold value, carrying out area growth, and obtaining all foreign matter reference points in the reference area; acquiring an overlapping coefficient of a non-foreign matter reference point in the reference area, acquiring a new seed point and a corresponding new growth threshold when the overlapping coefficient of the non-foreign matter reference point is greater than a preset overlapping coefficient threshold, performing area growth, and acquiring a new foreign matter reference point; all the foreign matter reference points and the new foreign matter reference points in the reference area form a foreign matter area of the reference area, the foreign matter area of the area where each capsule is located in the gray level image is obtained, and defect recognition is conducted on the fish oil soft capsule image. According to the invention, the complete foreign matter area can be rapidly obtained, and the apparent defect identification of the fish oil soft capsule is more accurate.

The present invention discloses a pet food containing fish-derived raw materials with added vitamins and having the efficacy of beautifying hair and skin care, and a preparation method thereof. The pet food containing fish-derived raw materials with added vitamins of the present invention comprises the following raw materials in parts by weight: 8-16 parts of salmon, 1-5 parts of salmon fish oil, 50-60 parts of chicken, 10-15 parts of chicken liver, 10-15 parts of chicken heart, 2-5 parts of sweet potato powder, 1-5 parts of brewer's yeast powder, 2-5 parts of chicken oil, 1-2 parts of psyllium powder, 1-2 parts of chicory root powder, 1-2 parts of cellulose, 0.5-2 parts of egg yolk lecithin, 0.2-0.8 parts of polyglycerol fatty acid ester, 5-20 parts of water, 0.2-0.5 parts of vitamin E, and 0.05-0.1 parts of agar. The pet food containing fish-derived raw materials with added vitamins of the present invention is nutritionally comprehensive, can enhance the physique of pets, help the growth of pet hair, and make the pet hair color bright and shiny.

The utility model discloses a fish oil distillation retort capable of increasing EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid) contents, and relates to the technical field of fish oil purification. The barrel body is arranged in the tank body; the pump assembly is used for adding a catalyst in the catalyst box into the barrel; and the stirring assembly is used for stirring the fish oil, the buffer solution and the catalyst in the barrel so as to form a mixed solution. After a catalyst in the catalyst box is fed into the barrel body by the pump assembly, fish oil, a buffer solution and enzyme are stirred and mixed by the stirring assembly, so that the reaction is accelerated, the enzyme catalyst is added into the barrel body in batches, the reaction process is adjusted more accurately, waste is avoided, and the cost of the enzyme catalyst is saved; and after stirring and heating reaction for a period of time, centrifuging the obtained mixture, standing for a period of time for liquid separation, and distilling the upper oil phase to obtain the fish oil with higher EPA and DHA contents.

The invention relates to the technical field of aquaculture feed, in particular to babylonia feed and a preparation method thereof. The babylonia lutosa feed is prepared from the following raw materials: fish meal, shrimp meal, soybean meal, wheat starch, corn starch, fish oil, vegetable oil, sodium alginate, carrageenan, betaine, an amino acid mixture, a vitamin premix, a mineral premix and an antioxidant. The feed can be dried and stored at normal temperature, and is convenient to store and transport; before being used, the feed is soaked in clear water for 30 minutes and can be swelled and jellied, so that babylonia can eat conveniently; meanwhile, the zero-dissolution-loss characteristic is achieved, and pollution to water quality and water bottom is reduced; a good food calling effect is achieved, and the feeding rate of babylonia can be increased; moreover, eating safety is guaranteed, healthy growth of Babylonia lutosa is promoted, and breeding benefits are improved.

The present invention relates to the field of food processing technology, and discloses a fish oil and a refining method thereof. The method comprises: (1) first mixing crude fish oil with water, a complex enzyme, and a chelating agent to obtain a mixed material; the complex enzyme is a combination of protease, phospholipase, and lipase; (2) sequentially separating and washing the mixed material to obtain an intermediate material I; (3) sequentially subjecting the intermediate material I to a first adsorption decolorization, a second adsorption decolorization, and a third adsorption decolorization to obtain an intermediate material II; the adsorption material I in the first adsorption decolorization comprises silica gel; the adsorption material II in the second adsorption decolorization is bleaching clay; and the adsorption material III in the third adsorption decolorization is activated carbon; (4) deodorizing the intermediate material II to obtain a fish oil product. The method provided by the present invention can effectively remove impurities in crude fish oil, retain a high rate of beneficial accompaniments in fish oil, and effectively control the generation of harmful substances in the production process.

This invention relates to the field of pet nutrition technology, and more particularly to a fresh dog food for improving coat health and its preparation method. The fresh dog food of this invention comprises component A and component B. Component A includes the following components in parts by weight: 40-45 parts venison, 10-12 parts mussels, 8-10 parts chicken liver, 8-10 parts duck egg, 3-5 parts carrot, 3-5 parts broccoli, 3-5 parts pumpkin, 3-5 parts taro, 3-5 parts avocado, 0.8-1 part dried red dates, 0.8-1 part dried rose petals, 0.5 parts whey calcium, 1.3 parts malt powder, 0.5 parts inulin, 0.5 parts brewer's yeast, and 0.2 parts fish oil. Component B is Bacillus subtilis; the viable count of Bacillus subtilis is 1×10⁻⁶. 9 CFU / g. This invention utilizes the synergistic effect of deep-sea shellfish and venison to improve the coat quality of puppies, increasing the coat diameter by ≥23.1% and the scale thickness by ≥32.4%.

The invention discloses a preparation method of phospholipid type DHA (docosahexaenoic acid) and application of the phospholipid type DHA in preparation of products for improving allergy symptoms, and belongs to the technical field of preparation and application of the phospholipid type DHA. Obtaining free DHA from the fish oil; soybean lecithin and free DHA are mixed, n-hexane is added for ultrasonic dissolution, enzyme is added after dissolution, nitrogen is filled for sealing for reaction, enzyme is removed after the reaction is finished, an organic solvent is removed from reaction liquid through rotary evaporation, mixed grease is obtained, and the phospholipid type DHA is obtained through purification. In the aspect of improving allergy, the phospholipid DHA prepared by the invention has a better mixing effect than fish oil and soybean lecithin, and has a good application prospect in the aspect of preparing functional foods, medicines or medicine carriers.

1. Name of the design product: Packaging bag (Shuran Full-price All-stage Fish Oil Cat Food, Dog Food). 2. Use of the design product: For packaging bags. 3. Design key points of the design product: The combination of shape and pattern. 4. Picture or photo that best shows the design key points: Perspective view of Design 1. 5. The design key points of the top views of Design 1, Design 2, Design 3, and Design 4 are shown in other views, so the top views of Design 1, Design 2, Design 3, and Design 4 are omitted. 6. Design 1 is designated as the basic design.

This invention discloses a pet food with coat-enhancing, shine-promoting, and coat-protecting effects, its preparation method, and applications. The food comprises the following components: chicken meal, fresh chicken meat, duck meal, sardine meal, chicken liver, sweet potato meal, corn meal, chicken heart, beef bone meal, duck meat pear, chicken oil, and egg yolk granules; coriander root powder, inulin, seaweed powder, beet pulp, flaxseed, fish oil, cranberry powder, carrot, krill, honeysuckle, turmeric, chrysanthemum, mannan oligosaccharide, lactulose, etc. The raw materials and auxiliary ingredients of this pet food are widely available, palatable, inexpensive, and readily available. The coriander root powder, other auxiliary ingredients, and nutritional supplements / additives work synergistically to improve the shine of the pet's coat, promote growth, and maintain coat health, while also improving the microstructure of the hair cuticle, fundamentally preventing pet coat health problems. The pet food preparation method disclosed in this invention is simple to operate, safe, and environmentally friendly, and is particularly suitable for feed products with high humidity and high heat sensitivity.

The invention discloses an automatic fish gelatin cleaning line, which belongs to the field of fish gelatin cleaning and comprises a gelatin soaking pool, a gelatin soaking chamber is arranged in the gelatin soaking pool, and a first elevator is arranged in the gelatin soaking chamber. According to the automatic fish gelatin cleaning line, the bubbling cleaning machine is arranged, multiple sets of stirring blades can be driven to rotate by starting an external motor so as to stir fish gelatin in the bubbling cleaning machine, flowing of fluid in the bubbling cleaning machine can be promoted by starting an aeration machine, aeration operation can be carried out on the fish gelatin, and the fish gelatin cleaning efficiency is improved. In the process that fluid in the bubbling cleaning machine flows, the contact effect between the fluid and the fish gelatin can be promoted, fish oil treatment on the fish gelatin can be further improved, and through the cleaning line, the fish gelatin can be subjected to gelatin soaking, powder mixing, flushing, cleaning, fish oil squeezing and high-pressure flushing and centrifugal spin-drying integrated operation.

The utility model discloses a mixing device for producing astaxanthin fish oil, and belongs to the field of fish oil production devices. Comprising a mixing tank body, an upper cover is arranged on the mixing tank body, and a feeding port used for feeding astaxanthin powder into the mixing tank body is formed in the upper cover; the fixed ring is fixed in the mixing tank body and is positioned on the upper side of the feeding hole; the rotating ring and the fixed ring are coaxially arranged, and the rotating ring is rotationally arranged on the fixed ring; the fixing frames are circumferentially fixed to the rotating ring, and the fixing frames are located on the lower side of the feeding opening; and the screening frame is arranged on the fixed frame in a sliding manner. The mixing device for astaxanthin fish oil production has the beneficial effects that through the arrangement of the rotating ring and the fixed frame, in the rotating process of the rotating ring, the fixed frame is driven to move, and meanwhile, the moving of the screening frame is matched, so that astaxanthin powder is uniformly screened out into the mixing tank body through the through holes in the screening frame.

The invention discloses bacillus subtilis 12-10-2 and application thereof in synergistic preparation of fish oil and fish sauce, and belongs to the technical field of microbial fermentation. The preservation number of the bacillus subtilis (Bacillus subtilis) 12-10-2 is CGMCC (China General Microbiological Culture Collection Center) No.34966. The bacillus subtilis (Bacillus subtilis) 12-10-2 can be used for preparing the bacillus subtilis. The bacillus subtilis 12-10-2 can be used for efficiently hydrolyzing protein in a high-salt environment. The bacillus subtilis 12-10-2 is used for performing bacterium-adding fermentation on the snakehead processing leftovers, so that the fish oil and the fish sauce can be synergistically prepared, and the obtained product is high in yield and good in quality. A new microbial resource is provided for high-value utilization of snakehead processing leftovers, the problems of environmental pollution and resource waste caused by improper treatment can be solved, a new technical support is provided for establishment of a preparation process of high-quality fish oil and fish sauce, and the method has a wide market application prospect.

The invention relates to the field of fish oil production, in particular to heating reaction equipment for fish oil production, which can ensure that a catalyst can be uniformly added into the equipment, can ensure uniform stirring, enables fish oil to react fully and avoids the problem that the local reaction rate is blocked, and comprises a reaction box, a reaction assembly is arranged in the reaction box, a feeding box is installed at one end of the top of the reaction box and located on the upper portion of the reaction assembly, and a driving motor is installed at the other end of the top of the reaction box. Through the arrangement of the catalysis box, the device can automatically add a catalyst, uniform addition is guaranteed, the accumulation phenomenon is avoided, through the mutual cooperation of the catalysis box and the reaction assembly, the multiple stirring function is achieved, the uniform stirring function is achieved, and through the uniform additive adding and uniform stirring functions, the reaction efficiency is improved. The fish oil reaction efficiency of the device can be guaranteed, and the phenomenon that local reaction is blocked or excessive reaction occurs is avoided.

The invention belongs to the technical field of fishery breeding, and provides a low-oxygen-resistant low-fish-meal compound feed as well as a preparation method and application thereof. The low-fish-meal compound feed is prepared from the following raw materials: fish meal, soybean meal, soybean protein isolate, flour, chicken meal, cassava starch, fish oil, soybean oil, lecithin, monocalcium phosphate, compound vitamins, compound minerals, choline, vitamin C, DL-Met, Lys-HCL, Thr, astaxanthin and chrysophyta laminarin. Firstly, the soybean meal, the soybean protein isolate and other composite plant proteins and the chicken meal are adopted to replace traditional fish meal, amino acid composition is balanced, the nutrition proportion of the compound feed is optimized, and the proportion of the fish meal is reduced to 20%; in addition, compound antioxidants such as astaxanthin and chrysophyta laminarin are added into the compound feed disclosed by the invention, so that the oxidation resistance of fish bodies can be remarkably improved.

The application discloses a method for preparing goldfish feed from fermented abalone viscera powder, and comprises the following steps: preparing fermented abalone viscera powder, including raw material pretreatment, strain activation, anaerobic fermentation and final treatment; preparing a formula of the goldfish feed, including fermented abalone viscera powder, spirulina powder, fish meal, soybean meal, rice bran, wheat flour, fish oil, alpha-starch, soybean lecithin, calcium dihydrogen phosphate, vitamin premix, mineral premix, betaine, garlicin, VC-phosphate, lysine and choline chloride; and preparing the goldfish feed, including accurate batching, uniform mixing, moisture adjustment and molding, grading granulation, drying and cooling and grading screening. The fermentation and feed preparation process is simplified, the complicated enzymolysis-fermentation combined process is abandoned, no professional technical personnel are needed, and the household breeders can prepare the feed through simple equipment, and the enterprises can mass-produce the feed; the formula is optimized for goldfishes in different growth stages, is suitable for multiple goldfishes such as golden goldfish and grass goldfish, and has high application flexibility.

The present invention discloses a fish oil processing tail material storage and distribution device, which relates to the field of fish oil processing, and comprises a base plate, a bracket and a hopper, wherein the base plate is fixed with a bracket, the bracket is fixed with a hopper, and the lower end of the hopper is installed with a distribution mechanism, and the distribution mechanism comprises a distribution pipe. The fish oil processing tail material storage and distribution device adopts a distribution mechanism to realize the material guiding function of fish oil tail material in different directions, thereby providing a basic guarantee for realizing continuous storage of tail material, and cooperates with a linkage blocking mechanism to open the baffle at the discharge port position on one side of the distribution pipe and close the baffle at the discharge port position on the other side during the distribution process. The open side of the baffle can realize an auxiliary material blocking function when the tail material is discharged, thereby preventing the tail material from splashing outward due to inertia. The closed side of the baffle can realize the blocking of the discharge port, thereby preventing the residual adhesive material in the distribution pipe from dripping and causing environmental pollution when the storage barrel is replaced, thereby better meeting the packaging requirements of the tail material.

This invention discloses a microfluidic method for preparing fish oil microspheres based on viscosity control and its application, belonging to the field of functional food preparation technology. The method involves constructing three viscosity systems (3.47–15.19 mPa·s) by compounding concentrated fish oil and castor oil at a specific volume ratio. Using a 3% polyvinyl alcohol aqueous solution as the continuous phase, water-in-oil fish oil microspheres are prepared under constant pressure in a cross-shaped microfluidic chip. By controlling the viscosity of the oil phase, the microsphere particle size can be precisely controlled within the range of 53.89–72.34 μm, with a coefficient of variation ≤5% and regular morphology. The obtained microspheres exhibit excellent particle size stability under pH 5–7 and 0–2% NaCl conditions. The high-viscosity microspheres show significantly lower acid values ​​and thiobarbituric acid reactant values ​​compared to the low-viscosity group under storage conditions of 4–60℃, with an oxidation stability improvement of over 30%. In vitro digestion experiments show that the low-viscosity microspheres reach peak free fatty acid release rate after 120 min, while the high-viscosity group exhibits a sustained-release effect. This invention achieves simultaneous optimization of fish oil microsphere particle size, stability, and digestive behavior, providing a theoretical basis and precise process parameters for the structured design of ω-3 polyunsaturated fatty acid functional foods.

The invention discloses an artificial bait additive for improving intestinal flora of yellow fin tuna, which comprises 15-30% of fish meal, 15-30% of soybean meal, 5-10% of wheat starch, 2-5% of fish oil, 1-3% of rapeseed oil, 5-10% of mulberry leaf powder, 5-15% of fly maggot powder, 1-5% of microalgae, 0.1-0.5% of bile acid, 1-3% of pine needle meal, 5-15% of brown rice and 1-5% of cow milk casein. The Chinese herbal medicine active components comprise 0.01%-0.1% of an acanthopanax senticosus extract, 0.05%-0.5% of ophiopogon japonicus polysaccharide, 1%-5% of bitter gourd dry powder, 0.01%-0.1% of a platycodin extract and 1%-5% of a fresh peach leaf fermentation product; the probiotic complexing agent comprises the following components in percentage by weight: 0.01%-0.5% of bacillus subtilis fermentation liquor, 0.01%-0.2% of lactobacillus acidophilus freeze-dried powder and 0.01%-0.3% of bacillus licheniformis fermentation liquor, and the auxiliary components comprise 1%-5% of a brewer's grain fermentation substrate, 0.1%-0.5% of a fish oil emulsifier and 0.1%-1% of fructo-oligosaccharide. According to the present invention, the growth performance of the yellow-fin tuna can be comprehensively promoted, the immunity is enhanced, the intestinal health of the yellow-fin tuna is optimized, and the feed conversion rate can be increased.

The invention belongs to the technical field of fish oil refining, and particularly relates to a fish oil purification method. Comprising the following steps: preparing specific free fatty acid adsorption starch; preparing oxygen vacancy carbon composite decolorized hydrotalcite; and refining and purifying the fish oil. The preparation method comprises the following steps: carrying out alkylation treatment on high-amylose corn starch, and subsequently carrying out cross-linking combination on the high-amylose corn starch, template molecule oleic acid of free fatty acid, 3-acrylamido phenylboronic acid, temperature-sensitive N-isopropylacrylamide and pentaerythritol triacrylate, so as to prepare the specific free fatty acid adsorption starch. According to the invention, the starch-lipid mixture can be generated with free fatty acid at a high temperature, the deacidification efficiency is improved, meanwhile, under the modification of an oleic acid specific template, the adsorption amount and adsorption efficiency of the specific free fatty acid adsorption starch on the free fatty acid can be further increased, and the free fatty acid in the fish oil can be efficiently removed; the peroxide value and the acid value of the purified fish oil are reduced.