242 results about "Polyglutamic acid" patented technology

The invention relates to the technical field of fertilizers, in particular to an environment-friendly water-soluble fertilizer containing a seaweed extract and polyglutamic acid. The water-soluble fertilizer is prepared by preparing seaweed oligosaccharide and double-grafted poly-L-gamma-sodium glutamate and compounding and complexing the seaweed oligosaccharide and the double-grafted poly-L-gamma-sodium glutamate, has the characteristics of low salting-out, high stability, no precipitation, good slow release performance and the like, and can continuously release nutrients and keep a solution clear and transparent under a low-salt condition; and because the polyglutamic acid and seaweed extract compound drip irrigation agent has the chelation and water retention characteristics of the polyglutamic acid and the natural growth promoting effect of the seaweed extract, the crop rhizosphere nutrient absorption efficiency and stress resistance are remarkably improved, the compound drip irrigation agent is suitable for drip irrigation and water and fertilizer integrated application of greenhouse vegetables, fruit trees and field crops, and the comprehensive effects of high efficiency, low salt load and environment friendliness are achieved.

The invention belongs to the technical field of membrane separation, and particularly relates to a low-pressure high-flux polyamide / polysulfone hollow fiber composite nanofiltration membrane and a preparation method thereof. The preparation method of the composite nanofiltration membrane comprises the following steps: dissolving sulfonated polysulfone, poly (1-vinyl-3-butylimidazole) bromide and polyvinylpyrrolidone to prepare a spinning solution, and spinning to obtain a base membrane; carrying out hydrophilic modification on the base membrane by using 1, 3-propane sultone; then, an aqueous phase solution containing poly (gamma-glutamic acid) and triethylene tetramine and an organic phase solution containing trimesoyl chloride and 3, 5-diaminobenzoic acid are subjected to interfacial polymerization in the inner cavity of the modified base membrane to form a polyamide separation layer; and finally, carrying out heat treatment to obtain the composite nanofiltration membrane. Through the synergistic effect of zwitterionic ionization of the base membrane and biomacromolecule construction of the separation layer, the mass transfer resistance of water is remarkably reduced, unification of high flux and high selectivity is achieved, and good structural stability is achieved.

The invention provides a polyglutamic acid granular fertilizer and a preparation method thereof, and the preparation method comprises the following steps: S1, dissolving lignin in an alkaline solution, and heating until the lignin is dissolved; adding epsilon-polylysine fermentation liquor, mixing, heating, adding a formaldehyde solution, and carrying out grafting reaction to obtain epsilon-polylysine grafted lignin; s2, mixing the epsilon-polylysine grafted lignin solution and the gamma-polyglutamic acid solution, adding N-hydroxysuccinimide, standing at a low temperature, adding 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and mixing; carrying out gradient temperature reaction to obtain lignin grafted epsilon-polylysine / gamma-polyglutamic acid hydrogel; and S3, homogenizing the lignin-grafted epsilon-polylysine / gamma-polyglutamic acid hydrogel, carrying out normal-temperature high-pressure airflow crushing, and spraying the crushed hydrogel to the surfaces of particles obtained by granulating a basic material through high-pressure electrostatic spraying, so as to obtain the polyglutamic acid granular fertilizer. The obtained polyglutamic acid granular fertilizer has relatively strong water-retaining and slow-release (releasing water, urea and the like) effects.

The invention discloses an organic-inorganic composite acid soil conditioner with synergism of nitrogen element synergism and soil expansion and application, and belongs to the technical field of soil conditioners. The modifier prepared by the invention is prepared from an acid neutralizer, a structure modifier, a capacity expansion synergist and a nitrogen synergist and is prepared from the following components in parts by weight: 18 to 22 parts of dolomite powder, 6 to 10 parts of lime powder, 20 to 24 parts of oyster shell powder, 8 to 12 parts of humic acid, 4 to 8 parts of sargassum, 1 to 3 parts of sodium alginate, 7 to 11 parts of charcoal, 5 to 9 parts of sepiolite powder, 3 to 7 parts of bentonite, 6 to 10 parts of poly gamma-calcium glutamate and 3 to 7 parts of chitosan. The soil conditioner can regulate and control the soil acidity, buffer the soil capacity, improve the soil structure and balance soil nutrients, solves the problems of high re-acidification rate, soil hardening and nutrient imbalance of a traditional lime conditioner, and is suitable for pHlt; and 6.5, repairing the acid soil.

The present application relates to the field of medical tissue adhesive, in particular to a medical tissue adhesive, a medical tissue dry adhesive patch and a preparation method and application thereof. The present application provides a medical tissue adhesive, a medical tissue dry adhesive patch and a preparation method and application thereof. The medical tissue adhesive provided by the present application comprises: 1wt%-30wt% of acidic polyglutamic acid; 1wt%-30wt% of spherical protein; and the balance of water. The medical tissue adhesive provided by the present application has high tissue adhesion strength and fast hemostatic sealing speed in a wet physiological environment, meets the requirements of rapid and efficient tissue hemostatic sealing, and can be applied to rapid hemostasis of tissue wounds in a wet physiological environment, sealing of wound liquid leakage, healing and repair of wound tissue, and antibacterial of wounds. Experiments show that the dry adhesive patch made of the adhesive provided by the present application has high tissue adhesion strength in a wet physiological environment, and the adhesion strength to a wet pigskin tissue can reach more than 60kPa.

The present application belongs to the technical field of biochar, and particularly relates to a high-activity acidolysis biochar, which is prepared by the following steps: S1, pretreating agricultural and livestock waste; S2, acidolysing the pretreated biomass with dilute acid to obtain acidolysed biomass; S3, placing the acidolysed biomass into a high-temperature furnace to perform carbonization under an oxygen-free condition, and using a step-by-step heating mode to obtain preliminary biochar; and S4, mixing the preliminary biochar with a copolymer solution to prepare the high-activity acidolysis biochar. In the present application, the sodium lignosulfonate-polyglutamic acid copolymer forms a stable connection with the biochar through chemical bonding, the porous surface of the biochar is uniformly covered with the copolymer to form a nanoscale film, the composite has stronger complexing, chelating and ion exchange capacity for heavy metal ions and other cations in the soil, and is helpful to reduce toxic substances in the soil and improve the soil environment.

The present invention relates to a cosmetic composition, advantageously an eco-biological cosmetic composition, comprising: - polyglutamic acid or a salt thereof; and - at least one peptide derivative represented by the general formula (I) in which: - R1 represents a hydrogen atom, an acyl radical or an acyloxy radical; and - R2 represents the side chain of an alpha-amino acid chosen from the group consisting of phenylalanine, glutamic acid, arginine, cysteine, methionine, histidine and tyrosine. The present invention also relates to the use of said cosmetic composition for combating skin redness and / or skin aging; to its use for preventing and / or combating cutaneous vascular hyperproliferation and / or cutaneous inflammation and / or rosacea; and to a method for selecting compounds suitable for preventing and / or combating these pathologies.

The invention provides a nano drug-loaded particle based on gamma-polyglutamic acid and epsilon-polylysine, a composite shell is arranged outside the nano drug-loaded particle, the composite shell is formed through a reaction of gamma-polyglutamic acid and epsilon-polylysine, and a single active component is loaded in the composite shell; the single active component is one of salicylic acid, gallic acid or tea polyphenol. The invention also provides a preparation method and application of the nano drug-loaded particle. According to the nanoparticles, a unified ionic crosslinking process is adopted, and three kinds of nanoparticles with gamma-polyglutamic acid and epsilon-polylysine as composite shells and different single active ingredients loaded on inner cores are prepared respectively. The nanoparticles provided by the invention are stable in structure and good in biocompatibility, and can be used for preparing medicines with anti-aging, antibacterial and anti-inflammatory effects respectively or in combination.

The invention relates to the technical field of microorganisms, in particular to an Exiguobacterium aceticum and application thereof. The Exiguobacterium aceticum strain is classified and named as Exiguobacterium aceticum, the strain number is H102, the Exiguobacterium aceticum strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 24, 2025, and the preservation number is CGMCC No.37157. The Exiguobacterium aceticum strain has the advantages that the Exiguobacterium aceticum strain can be used for preparing the Exiguobacterium aceticum strain; the strain has strong saline-alkaline tolerance, drought tolerance and high temperature resistance, and a foundation is provided for colonization of the strain. Meanwhile, the strain has the functional characteristics of producing acetoin, polysaccharide and polyglutamic acid and the like, can effectively provide nutritional ingredients for plant growth and reduce the harm of abiotic stress to the plants, and therefore, the strain has important application value in the aspects of improving saline-alkali soil, improving abiotic stress resistance of the plants, promoting plant growth and the like.

This invention discloses a core-shell structured slow-release microbial fertilizer for grapes and its preparation method, belonging to the field of microbial slow-release fertilizer technology. The invention prepares three-layer core-shell structured microbial fertilizer granules, consisting of a microbial core layer, a nutrient layer, and a shell layer, from the inside out. The microbial core layer contains a complex microbial community, modified γ-polyglutamic acid, and trehalose; the nutrient layer is compounded with fast-acting nitrogen, phosphorus, and potassium fertilizers, and slow-release nitrogen fertilizer; the shell layer uses a composite system of modified γ-polyglutamic acid, nano-montmorillonite, and grape pomace biochar. This invention constructs an ion barrier through dual modification of γ-polyglutamic acid with phosphate groups and amino groups, improving the survival rate of the microbial strain and its rhizosphere colonization ability. Simultaneously, utilizing the protonation characteristics of amino groups at pH, a positive feedback loop of rhizosphere microenvironment-responsive fertilizer release is constructed. Combined with nano-montmorillonite and grape pomace biochar, the granule's mechanical strength and slow-release performance are enhanced, soil structure is improved, and the resource recycling of grape processing waste is achieved.

This invention discloses a method for determining the content and simultaneously identifying the source of polyglutamic acid (PCA) in fertilizers based on microwave digestion-high-resolution liquid chromatography-mass spectrometry (LC-MS), belonging to the field of analytical detection technology. The method includes: microwave-assisted acid hydrolysis of fertilizer samples to obtain a hydrolysate; injecting the hydrolysate into a liquid chromatography-high-resolution mass spectrometry (LC-MS) instrument, performing a single injection analysis in data-independent acquisition (DIA) mode, and simultaneously acquiring a mass spectrometry dataset containing a full-scan fingerprint of the parent ion and fragment information of the daughter ion by alternating between low and high collision energies; extracting the precise mass number of PCA from the dataset for accurate quantification, and calculating the PCA content using the difference method; simultaneously retrieving the total ion chromatogram and inferring the source of PCA based on the response of other amino acids. This invention is the first to introduce microwave digestion pretreatment and high-resolution mass spectrometry DIA acquisition mode into the field of PCA detection in fertilizers, requiring only a single injection to simultaneously complete accurate quantification and source identification, effectively avoiding false positives caused by background of plant and animal protein hydrolysates, and significantly improving the efficiency and confidence of PCA detection in complex matrix fertilizers.

The invention provides a medical beauty-grade sensitive skin repair dressing with a gamma-polyglutamic acid controlled release function, and belongs to the technical field of biomedical materials. The repair dressing is composed of controlled release microspheres dispersed in a matrix material, the controlled release microspheres take modified gamma-polyglutamic acid as an active core material and a biocompatible high polymer material as a wall material, and the matrix material comprises a medical grade gel matrix, a moisturizing component and an anti-inflammatory soothing component. The gamma-polyglutamic acid is subjected to acylation or graft modification to improve the stability and biocompatibility of the gamma-polyglutamic acid, and 12-72h gradient release of the gamma-polyglutamic acid is realized by virtue of the controlled release microspheres prepared by virtue of an emulsification-crosslinking method or a solvent evaporation method; meanwhile, through the synergistic matching of the matrix components, the dressing is endowed with the moisturizing, anti-inflammatory and soothing effects. The dressing can effectively reduce wound inflammatory response after medical art, promote epidermal cell proliferation and improve the water content and barrier function of a sensitive skin cuticle, and is suitable for scenes of wound repair after medical art, sensitive skin barrier reconstruction and the like.

The invention relates to the technical field of fertilizers, in particular to a nutrient polymerized polyglutamic acid compound fertilizer and a preparation method thereof, and the nutrient polymerized polyglutamic acid compound fertilizer comprises the following raw materials in parts by weight: 6-10 parts of gamma-polyglutamic acid, 25-30 parts of filler, 20-25 parts of a nitrogen source, 12-15 parts of a phosphorus source, 10-14 parts of a potassium source, 4-6 parts of a compound additive and 0.1-0.2 part of a fungicide. In the preparation of the granules, the humic acid powder is coated by a first treating agent prepared from the soluble starch, the salicylic acid, the sodium alginate and the like, so that the activity of the humic acid is effectively protected, and meanwhile, the mechanical strength and the slow-release characteristic of the granules are further enhanced by a second treating agent prepared from the components such as the chitosan and the fulvic acid through a chelation cross-linking effect; in addition, chondroitin sulfate, calcium nitrate, ammonium molybdate and molasses in the batch jointly provide medium trace elements and organic carbon sources, and synchronous release and efficient utilization of nutrients after the fertilizer is applied to soil are ensured.

This invention discloses a slow-release fertilizer suitable for lateritic soils in Hainan, comprising a fertilizer core and a coating layer; the fertilizer core comprises the following raw materials in parts by weight: 10-20 parts isobutylene diurea, 1-3 parts 3,4-dimethyl-1H-pyrazole dihydrogen phosphate, 1-3 parts dicyandiamide DCD, 20-30 parts humic acid, and 30-50 parts organic fertilizer; the coating layer comprises the following raw materials in parts by weight: 5-10 parts natural nanotube halloysite, 3-5 parts bentonite, 5-10 parts cyclodextrin, 1-3 parts polyglutamic acid, 1-3 parts wood vinegar, and 5-10 parts polyacrylamide. The isobutylene diurea, humic acid, and organic fertilizer of this invention can effectively replenish organic matter and nitrogen in iron-aluminate soil, maintain soil nitrogen levels, and effectively control the conversion of ammonium nitrogen to nitrate nitrogen in lateritic soil, thereby reducing nitrogen loss and achieving a stable supply of nitrogen. In addition, polyglutamic acid and polyacrylamide have strong water retention capacity, and the fertilizer core is coated with a coating layer to achieve slow release and water retention.

The application discloses a kind of microbial fertilizer and its in improving white pulp soil carbon sequestration capacity and application in promoting straw decomposition, belong to the technical field of microbial fertilizer.The microbial fertilizer includes organic matter, biochar, microbial inoculant, trace element and microbial activator;The microbial inoculant includes bacillus ND2 and bacillus megaterium CICC 22681;The microbial activator includes fulvic acid, gamma-polyglutamic acid and sorbitol.The microbial fertilizer of the application has the dual function of white pulp soil improvement and straw decomposition, can accelerate straw decomposition, improve white pulp soil structure, improve soil fertility and carbon sequestration capacity, help agricultural waste resourceization and soil quality improvement collaborative development, in white pulp soil area or other barren soil area in future agricultural production, it is expected to become important technical support for promoting sustainable development of ecological agriculture, and application prospect is wide.

This invention relates to the field of microbial fermentation, specifically to a method for promoting the fermentation of Bacillus atrophaeus to produce γ-polyglutamic acid (PGA) using oxygen carriers. This invention involves adding specific alkane oxygen carriers during the initial stage of γ-PGA production via Bacillus atrophaeus NWS-01 fermentation. Hexane, heptane, and hexadecane were selected as effective oxygen carriers, and the optimal single-addition concentration and optimal synergistic addition ratio of each oxygen carrier were determined. This solves the problem of limited γ-PGA yield due to low oxygen transfer efficiency in traditional fermentation processes. Specifically, adding 0.3% heptane alone achieves a γ-PGA yield of 39.4 ± 0.19 g / L, while the synergistic addition of 0.240% hexane, 0.407% heptane, and 1.014% hexadecane results in a maximum yield of 42.1 g / L, representing a 150.6% increase compared to the fermentation system without added oxygen carriers. This method is simple to operate, has high oxygen utilization, low energy consumption, and produces fewer bubbles and lower liquid shear force in the fermentation system, making it suitable for the industrial-scale aerobic high-density fermentation production of γ-PGA.

The application discloses a kind of anti-reverse tolerance of poor root flower planting slow-release fertilizer and its preparation method, the slow-release fertilizer includes 30-45 parts of seaweed residue, humic acid 15-25 parts, diatomite 10-20 parts, oyster powder 5-15 parts, plant protein glue 3-8 parts, polyglutamic acid 1-3 parts, bacillus mucilaginosus powder 0.5-2 parts, brown algal oligosaccharide 0.1-0.5 parts, potassium silicate 2-5 parts, ammonium molybdate 0.05-0.15 parts, silicon phytic acid ester 0.5-2 parts, by introducing silicon phytic acid ester, fertilizer particles can be adjusted according to soil humidity release rate of nutrients, lock up nutrients in drought, accelerate release in wet; Absorption and fixation harmful metal ions in soil, reduce stress, and slowly hydrolyze in slow-release process, continuously provide beneficial substances, synergistically improve the fertilizer utilization rate of root flower in poor soil, resistance tolerance and sustained growth capacity.

The invention discloses a water-soluble fertilizer for promoting swelling and coloring of crop fruits. The water-soluble fertilizer is prepared from the following raw materials: 30 to 50 parts of herba portulacae fermentation liquor, 15 to 25 parts of potassium nitrate, 10 to 20 parts of monopotassium phosphate, 2 to 8 parts of chelated trace elements, 2 to 4 parts of polyglutamic acid, 1 to 3 parts of compound sodium nitrophenolate and 1 to 3 parts of betaine. The invention belongs to the technical field of agricultural fertilizers, and particularly provides a water-soluble fertilizer for promoting fruit swelling and coloring of crops, which is characterized in that inert precursor substances in purslane are converted into efficient active components by a fermentation technology, and alpha-linolenic acid derivatives generated by fermentation of the purslane stimulate expression of relaxation proteins in fruit cell walls to achieve a fruit swelling effect.

The invention discloses a polyglutamic acid hemostatic hydrogel and a preparation method thereof. The polyglutamic acid hemostatic hydrogel comprises a phthalic dialdehyde grafted polyglutamic acid derivative and an adipic dihydrazide grafted polyglutamic acid derivative, the o-phthalaldehyde grafted polyglutamic acid derivative has a structure as shown in a formula 1 or a formula 3; the adipic dihydrazide grafted polyglutamic acid derivative has a structure as shown in a formula 2 or a formula 4. The polyglutamic acid hemostatic hydrogel provided by the invention comprises two components, i.e., a phthalic dialdehyde grafted polyglutamic acid derivative and an adipic dihydrazide grafted polyglutamic acid derivative, and polyglutamic acid is a biodegradable high polymer material with good biocompatibility; the hydrogel can realize rapid gelation through rapid reversible cross-linking of o-phthalaldehyde and hydrazino and hydrogen-bond interaction of a main chain of polyglutamic acid, and has excellent mechanical properties and adhesion strength.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

The invention relates to the technical field of fertilizer production and industrial solid waste resource utilization, and particularly discloses a method for producing a urea formaldehyde polyglutamic acid compound fertilizer from phosphorus and potassium mixed acid raffinate. According to the method, phosphorus-potassium mixed acid slurry, polyglutamic acid and urea formaldehyde are used as main raw materials, and the preparation method comprises the steps of raw material reaction and mixed acid preparation, multi-stage neutralization and functional agent mixing, slow-release nitrogen source mixing, returned material pretreatment and granulation, screening and wrapping, process waste heat recovery and the like. The product disclosed by the invention can be applied as a special compound fertilizer with nutrient slow release and biological stimulation functions, and has the advantages of balanced nutrients, high utilization rate and capability of improving soil. Besides, according to the preparation method, high-valued resource utilization of the phosphorus-potassium mixed acid raffinate is achieved, and through ammoniation pretreatment of returned materials and systematic recovery of reaction waste heat, the preparation method has the advantages that the raw material cost is reduced, the product performance is improved, and the production energy consumption is reduced.

The invention provides temperature-controlled hydrogel and a preparation method thereof, and belongs to the technical field of compositions of high-molecular compounds. The preparation method of the temperature control hydrogel comprises the following steps: preparing a polyglutamic acid-metacrylic acid ester copolymer, preparing composite chitosan, modifying fullerene with chitosan, and mixing. According to the temperature control hydrogel prepared by the invention, the swelling ratio of the temperature control hydrogel is 19.97-21.72 g / g, the swelling ratio of the hydrogel is high, the hydrophilicity of a hydrogel network is strong, the fullerene dispersity is good, and the biocompatibility is enhanced; the compression strength of the temperature control hydrogel ranges from 149.7 kPa to 152.3 kPa, the compression modulus ranges from 25.9 kPa to 28.6 kPa, the fracture deformation ranges from 70.5% to 73.1%, the mechanical performance of the temperature control hydrogel is excellent, and the critical solution temperature of the prepared hydrogel ranges from 31.12 DEG C to 31.35 DEG C.

The invention provides a bone adhesion solid-phase composition, a bone adhesive as well as a preparation method and application of the bone adhesive, and belongs to the technical field of bone repair materials. The invention provides a bone bonding solid-phase composition which comprises the following components in percentage by mass: 5-60% of phosphoserine, 10-80% of calcium phosphate and 5-50% of polyglutamic acid. According to the invention, by adding the polyglutamic acid and utilizing the chelation of the polyglutamic acid and calcium ions, the polyglutamic acid is adsorbed on the surface of the powder to form steric hindrance, the viscosity is improved, the ion diffusion is delayed, the reaction rate of calcium phosphate and phosphoserine is reduced, and the curing time is prolonged; meanwhile, due to the viscosity, the early-stage viscosity and the overall compressive strength of the material are enhanced. The result of the embodiment shows that the putty time of the bone adhesive prepared from the bone adhesive solid-phase composition provided by the invention can reach 20-70 minutes, and the adhesive strength and compressive strength are remarkably enhanced.

The invention discloses a quick-dry antibacterial compound for a packaging material as well as a preparation method and application of the quick-dry antibacterial compound, and relates to the field of packaging materials. The antibacterial compound is formed by high-molecular-weight gamma-polyglutamic acid and hexadecyl trimethyl ammonium chloride through an ionic bonding reaction and has a comb-shaped structure with gamma-PGA as a framework and HTAC combined through an ionic bond, hydrophobic long chains of the HTAC are arranged outwards to form a hydrophobic micro-area, and the hydrophobic micro-area is in a comb-shaped structure with the high-molecular-weight gamma-polyglutamic acid and the high-molecular-weight hexadecyl trimethyl ammonium chloride. An ethanol solution of the compound can be dried to constant weight on the surface of a packaging base material within 30 minutes, the drying efficiency is improved by 87% or above compared with that of pure gamma-PGA, and meanwhile, the bacteriostasis rates of the compound on escherichia coli and staphylococcus aureus are not lower than 90%. The invention further provides a specific preparation method of the compound and application of the compound in a packaging material, especially application of the compound in an antibacterial coating of a rapid processing production line, and the technical problem that when gamma-PGA is applied in the packaging field, the drying speed is low, and the antibacterial requirement is difficult to consider is successfully solved.

The application discloses a preparation method of drought-resistant polyglycoside synergistic fertilizer, and belongs to the technical field of agricultural fertilizer.The gamma-polyglutamic acid from microbial fermentation and the iridoid glycosides from plant extraction are chemically bonded at the molecular level by two-stage gradient induction self-assembly technology to prepare polyglycoside compounds with a three-dimensional network structure, and then the polyglycoside compounds are compounded with fertilizer base materials and granulated by a high tower melting to obtain the drought-resistant polyglycoside synergistic fertilizer.The polyglycoside compounds prepared by the application form a stable three-dimensional network structure through ester bonds and hydrogen bonds, have physical water retention and biological induction dual functions, and the water retention rate in soil is increased by 30%-50% compared with common fertilizer, the corn yield is increased by 20%-30%, and the fertilizer utilization rate can be increased by 18.8%, so that the drought-resistant synergistic effect is remarkable and the industrial application value is high.

The invention relates to a methanophilic genetically engineered bacterium for producing gamma-polyglutamic acid (gamma-PGA) as well as a construction method and application of the methanophilic genetically engineered bacterium, EQU2420670 gene is knocked out on a host bacterium genome, and meanwhile, an exogenous gamma-PGA synthetase encoding gene cluster pgsBCAE under the control of an inducible arabinose promoter (Pbad) is introduced at the site. The construction method comprises the following steps: obtaining a multi-cistron structure DNA fragment pgsBCAE for coding gamma-PGA synthetase through artificial gene synthesis in combination with a codon optimization strategy, and constructing the expression module Pbad-pgsBCAE under the control of a Pbad promoter. A suicide vector pK18mobsacB carrying a SacB sucrose reverse selection system is used for constructing a gene replacement vector, upstream and downstream homologous arms of an EQU2420670 gene on a genome of methanophilic bacteria M. buryatase 5GB1 are designed on two sides, an EQU2420670 site is replaced by a Pbad-pgsBCAE expression module, the methanophilic genetically engineered bacterium PGA is obtained, and the methanophilic genetically engineered bacterium PGA is applied to production of gamma-PGA and has important economic and social significance; the gamma-PGA produced by the engineering methanophilic bacteria by using methane as a carbon source and an energy source can be used as a cosmetic additive; the invention provides a novel low-carbon biosynthesis approach for commercial production and application of gamma-PGA, and has a good industrial application prospect.

The invention discloses a fertilizer synergistic modifier and a processing method thereof. The fertilizer synergistic modifier comprises 40-60 parts of a nutrient regulation and intelligent controlled release component; 1 to 15 parts of a photosensitive synergistic component; 25-45 parts of a carrier, a structure auxiliary agent and a coating material; wherein the nutrient regulation and control and intelligent controlled release component comprises a urease inhibitor, such as N-butyl thiophosphoric triamide (NBPT), a nitrification inhibitor, such as 3, 4-dimethyl pyrazole phosphate (DMPP), humic acid or a salt thereof, such as potassium humate, polyglutamic acid, a seaweed extract, a phosphate-solubilizing / potassium-solubilizing microbial agent, such as bacillus subtilis, and the like. And modified zeolite or mesoporous silica loaded with nitrogen, phosphorus, potassium and trace elements. The invention relates to the technical field of agricultural chemical fertilizers, and provides an intelligent controlled-release fertilizer, which realizes the synergistic interaction of external maintenance, internal strong absorption and environmental adaptation through systematic compounding of mature synergistic components, nano materials with clear photochemical characteristics and intelligent controlled-release carriers, and aims to significantly improve the fertilizer utilization rate, crop yield and stress resistance.

The invention discloses a functional fertilizer for improving drought resistance of crops in a shajiang black soil area and a production method, and relates to the technical field of functional fertilizers. The functional fertilizer is prepared from the following raw materials in parts by mass: 70 parts of humic acid, 22 parts of a fermented organic fertilizer, 20 parts of geopolymer particles, 20 parts of crop straws, 12 parts of organic adsorption particles, 6 parts of polymer tackifying powder, 2.5 parts of a microbial compound inoculant, 5-15 parts of molasses synergistic liquid and 0.5-2 parts of polyglutamic acid. Wherein the molasses synergistic liquid is viscous liquid, and the total sugar content of the molasses synergistic liquid is greater than or equal to 20%; the polyglutamic acid is powdery, and the purity is greater than or equal to 85%; the polyglutamic acid comprises low-molecular-weight polyglutamic acid and high-molecular-weight cross-linked polyglutamic acid; the molasses synergistic liquid and polyglutamic acid with different molecular weights are introduced to form slow-release synergistic gel, so that the water-retaining property, the structural stability, the pesticide adsorption and retention capacity and the microbial activity of the shajiang black soil are synergistically improved, and the drought resistance of crops is remarkably enhanced.

The invention relates to the technical field of environmental remediation materials, in particular to a hydrogel-based curing agent as well as a preparation method and application thereof. The hydrogel-based curing agent provided by the invention can repair engineering construction repair scenes prone to water and soil loss for a long time at a time, and is short in repair period, low in cost and good in repair effect. Specifically, the invention discloses a bio-based ionic cross-linked hydrogel curing agent, sodium alginate and chitosan are taken as base materials, the structural stability is enhanced through double-particle-size nano bentonite, and a water erosion resistant curing shell layer can be quickly formed by combining the fertilizer retention function of polyglutamic acid, so that long-acting repair of a red clay slope is realized, the repair period is short, the cost is low, and the curing agent has a wide application prospect. The method is suitable for water and soil loss prevention and control projects.

This invention discloses an alkaline, phosphorus-free nano-chelating agent, which, by weight percentage, is composed of the following components: 30%–40% sodium hydroxide, 6%–15% polyethylene terephthalate, 5%–10% polyglutamic acid, 1%–5% polysuccinimide, 11%–18% ammonium citrate, 2%–9% nano-silica, 2%–9% nano-alumina, and the balance being water. This phosphorus-free nano-chelating agent contains no phosphorus and will not cause phosphorus pollution during use. Compared to traditional phosphorus-containing chemicals, it is more environmentally friendly and helps reduce phosphorus content in water bodies, thereby mitigating environmental problems such as eutrophication.