96 results about "Polyglutamic acid" patented technology

The present invention relates to a cosmetic composition, advantageously an eco-biological cosmetic composition, comprising: - polyglutamic acid or a salt thereof; and - at least one peptide derivative represented by the general formula (I) in which: - R1 represents a hydrogen atom, an acyl radical or an acyloxy radical; and - R2 represents the side chain of an alpha-amino acid chosen from the group consisting of phenylalanine, glutamic acid, arginine, cysteine, methionine, histidine and tyrosine. The present invention also relates to the use of said cosmetic composition for combating skin redness and / or skin aging; to its use for preventing and / or combating cutaneous vascular hyperproliferation and / or cutaneous inflammation and / or rosacea; and to a method for selecting compounds suitable for preventing and / or combating these pathologies.

This invention discloses a core-shell structured slow-release microbial fertilizer for grapes and its preparation method, belonging to the field of microbial slow-release fertilizer technology. The invention prepares three-layer core-shell structured microbial fertilizer granules, consisting of a microbial core layer, a nutrient layer, and a shell layer, from the inside out. The microbial core layer contains a complex microbial community, modified γ-polyglutamic acid, and trehalose; the nutrient layer is compounded with fast-acting nitrogen, phosphorus, and potassium fertilizers, and slow-release nitrogen fertilizer; the shell layer uses a composite system of modified γ-polyglutamic acid, nano-montmorillonite, and grape pomace biochar. This invention constructs an ion barrier through dual modification of γ-polyglutamic acid with phosphate groups and amino groups, improving the survival rate of the microbial strain and its rhizosphere colonization ability. Simultaneously, utilizing the protonation characteristics of amino groups at pH, a positive feedback loop of rhizosphere microenvironment-responsive fertilizer release is constructed. Combined with nano-montmorillonite and grape pomace biochar, the granule's mechanical strength and slow-release performance are enhanced, soil structure is improved, and the resource recycling of grape processing waste is achieved.

This invention discloses a method for determining the content and simultaneously identifying the source of polyglutamic acid (PCA) in fertilizers based on microwave digestion-high-resolution liquid chromatography-mass spectrometry (LC-MS), belonging to the field of analytical detection technology. The method includes: microwave-assisted acid hydrolysis of fertilizer samples to obtain a hydrolysate; injecting the hydrolysate into a liquid chromatography-high-resolution mass spectrometry (LC-MS) instrument, performing a single injection analysis in data-independent acquisition (DIA) mode, and simultaneously acquiring a mass spectrometry dataset containing a full-scan fingerprint of the parent ion and fragment information of the daughter ion by alternating between low and high collision energies; extracting the precise mass number of PCA from the dataset for accurate quantification, and calculating the PCA content using the difference method; simultaneously retrieving the total ion chromatogram and inferring the source of PCA based on the response of other amino acids. This invention is the first to introduce microwave digestion pretreatment and high-resolution mass spectrometry DIA acquisition mode into the field of PCA detection in fertilizers, requiring only a single injection to simultaneously complete accurate quantification and source identification, effectively avoiding false positives caused by background of plant and animal protein hydrolysates, and significantly improving the efficiency and confidence of PCA detection in complex matrix fertilizers.

The application discloses a kind of microbial fertilizer and its in improving white pulp soil carbon sequestration capacity and application in promoting straw decomposition, belong to the technical field of microbial fertilizer.The microbial fertilizer includes organic matter, biochar, microbial inoculant, trace element and microbial activator;The microbial inoculant includes bacillus ND2 and bacillus megaterium CICC 22681;The microbial activator includes fulvic acid, gamma-polyglutamic acid and sorbitol.The microbial fertilizer of the application has the dual function of white pulp soil improvement and straw decomposition, can accelerate straw decomposition, improve white pulp soil structure, improve soil fertility and carbon sequestration capacity, help agricultural waste resourceization and soil quality improvement collaborative development, in white pulp soil area or other barren soil area in future agricultural production, it is expected to become important technical support for promoting sustainable development of ecological agriculture, and application prospect is wide.

The application discloses a kind of anti-reverse tolerance of poor root flower planting slow-release fertilizer and its preparation method, the slow-release fertilizer includes 30-45 parts of seaweed residue, humic acid 15-25 parts, diatomite 10-20 parts, oyster powder 5-15 parts, plant protein glue 3-8 parts, polyglutamic acid 1-3 parts, bacillus mucilaginosus powder 0.5-2 parts, brown algal oligosaccharide 0.1-0.5 parts, potassium silicate 2-5 parts, ammonium molybdate 0.05-0.15 parts, silicon phytic acid ester 0.5-2 parts, by introducing silicon phytic acid ester, fertilizer particles can be adjusted according to soil humidity release rate of nutrients, lock up nutrients in drought, accelerate release in wet; Absorption and fixation harmful metal ions in soil, reduce stress, and slowly hydrolyze in slow-release process, continuously provide beneficial substances, synergistically improve the fertilizer utilization rate of root flower in poor soil, resistance tolerance and sustained growth capacity.

This invention provides a cell microenvironment stabilizer that captures hydrogen ions, its preparation method, and its application, belonging to the field of biomedical technology. The preparation method of this cell microenvironment stabilizer includes the following steps: (1) mineralizing CaCl2 with TGF-β1, catalase, and polyethylene glycol-polyglutamic acid block copolymer to obtain mineralized nanoparticles; (2) preparing hydrogel microspheres under freezing conditions using a microfluidic device, and then photocrosslinking the frozen hydrogel microspheres; (3) mixing the hydrogel microspheres with the mineralized nanoparticles to prepare the cell microenvironment stabilizer. This invention prepares a mineralized hydrogel microsphere, which, as a cell microenvironment stabilizer, can be used to block the NLRP3 cascade in chronic inflammation, capturing excess hydrogen ions through the mineralization layer to neutralize the acidic microenvironment. This micro / nano bioreactor based on hydrogel microspheres can effectively interfere with NLRP3 in the microenvironment of degenerated tissues from inside and outside the cells, inhibiting chronic inflammation.

The application discloses a preparation method of drought-resistant polyglycoside synergistic fertilizer, and belongs to the technical field of agricultural fertilizer.The gamma-polyglutamic acid from microbial fermentation and the iridoid glycosides from plant extraction are chemically bonded at the molecular level by two-stage gradient induction self-assembly technology to prepare polyglycoside compounds with a three-dimensional network structure, and then the polyglycoside compounds are compounded with fertilizer base materials and granulated by a high tower melting to obtain the drought-resistant polyglycoside synergistic fertilizer.The polyglycoside compounds prepared by the application form a stable three-dimensional network structure through ester bonds and hydrogen bonds, have physical water retention and biological induction dual functions, and the water retention rate in soil is increased by 30%-50% compared with common fertilizer, the corn yield is increased by 20%-30%, and the fertilizer utilization rate can be increased by 18.8%, so that the drought-resistant synergistic effect is remarkable and the industrial application value is high.

This invention discloses an alkaline, phosphorus-free nano-chelating agent, which, by weight percentage, is composed of the following components: 30%–40% sodium hydroxide, 6%–15% polyethylene terephthalate, 5%–10% polyglutamic acid, 1%–5% polysuccinimide, 11%–18% ammonium citrate, 2%–9% nano-silica, 2%–9% nano-alumina, and the balance being water. This phosphorus-free nano-chelating agent contains no phosphorus and will not cause phosphorus pollution during use. Compared to traditional phosphorus-containing chemicals, it is more environmentally friendly and helps reduce phosphorus content in water bodies, thereby mitigating environmental problems such as eutrophication.

This invention belongs to the field of bioengineering technology and discloses a Bacillus licheniformis strain with enhanced yobN gene expression and its application in poly-γ-PGA synthesis. This Bacillus licheniformis strain with enhanced yobN gene expression is obtained by introducing a recombinant expression vector into a native Bacillus licheniformis strain, namely Bacillus licheniformis WX-02. The yobN gene is derived from Bacillus licheniformis WX-02, and its nucleotide sequence is shown in SEQ ID NO.1. This invention provides a novel metabolic engineering strategy for increasing γ-PGA production by constructing a recombinant expression vector carrying the yobN gene and transferring this recombinant expression vector into Bacillus licheniformis to promote poly-γ-glutamic acid (γ-PGA) synthesis. Compared with the empty vector control strain WX-02 / pHY300-P43u12, the recombinant Bacillus licheniformis WX-02 / pHY-yobN constructed in this invention showed an 89.4% increase in γ-PGA production, reaching 6.99 g / L.

This invention discloses a phosphate-solubilizing bacterium, 4P-1, and its drought-resistant phosphate-solubilizing agent. The phosphate-solubilizing bacterium 4P-1 is classified and named *Pseudomonas extremorientalis* 4P-1. The *Pseudomonas extremorientalis* 4P-1 strain provided by this invention possesses highly efficient phosphate-solubilizing ability. The addition of a drought-resistant agent prepared from specific substances (a mixture of polyglutamic acid and trehalose in a molar ratio of 3:1) significantly enhances the drought resistance of 4P-1 and its phosphate-solubilizing efficiency under drought conditions. Under the same drought stress level, the addition of the drought-resistant agent significantly improves the growth and phosphate-solubilizing ability of the 4P-1 inoculum in arid environments. This invention can provide technical support for the development and application of phosphate-solubilizing and drought-resistant microbial fertilizers for crops, promoting the growth of economic crops.

The application relates to the technical field of surgical biomaterials, and discloses an ultrasonic-responsive piezoelectric hydrogel as well as a preparation method and application thereof.The ultrasonic-responsive piezoelectric hydrogel comprises a conductive hydrogel base body and gallium selenide nanosheets dispersed and loaded in the conductive hydrogel base body; and the conductive hydrogel base body is formed by cross-linking hyperbranched polyglycidol grafted with gamma-polyglutamic acid and polyvinyl alcohol through hydrogen bonding.Under ultrasonic excitation, the gallium selenide nanosheets convert mechanical energy into a microelectric field through a piezoelectric effect and synchronously catalyze hydrogen production from water / glucose, while releasing gallium ions, which synergistically play multiple therapeutic roles: the microelectric field promotes fibroblast proliferation, migration and vascular regeneration, hydrogen gas removes active oxygen in an oxidative stress environment and improves a diabetic wound microenvironment, and the gallium ions realize efficient antibacterial effect on methicillin-resistant Staphylococcus aureus by interfering with bacterial iron metabolism, thereby constructing a response-type treatment system integrating antibacterial effect, antioxidant effect and healing promotion.

The application provides a pseudomonas koreensis WF3 and a saline-alkali soil microbial fertilizer, a preparation method and application thereof, and belongs to the technical field of biological composting.The preparation method of the saline-alkali soil microbial fertilizer provided by the application comprises the following steps: (1) configuring a fertilizer carrier comprising matured organic fertilizer, humic acid, attapulgite powder and polyglutamic acid; (2) inoculating the pseudomonas koreensis WF3 into the fertilizer carrier, and carrying out sealed fermentation to obtain the saline-alkali soil microbial fertilizer.The pseudomonas koreensis WF3 has the abilities of producing extracellular polysaccharides and organic acids, and can survive in a strong saline-alkali environment; the fertilizer carrier is fermented by the pseudomonas koreensis WF3; the prepared microbial organic fertilizer can effectively improve the soil organic carbon, total nitrogen and water-stable large aggregate (>2.5mm) content, increase the corn yield, reduce the soil pH and total salt content, and has a remarkable improvement effect on the saline-alkali soil.

This invention discloses a pH-responsive VA crosslinked γ-PGA / PVA interpenetrating network-embedded Sa@ZIF-8 hydrogel, its preparation method, and its application. The preparation method includes the following steps: (1) preparing a Sa@ZIF-8 aqueous solution; (2) adding an aqueous solution containing γ-polyglutamic acid and vanillin to a PVA aqueous solution and stirring to disperse it evenly; (3) slowly adding the Sa@ZIF-8 aqueous solution to step (2) and continuing to stir the reaction for 20-50 min; (4) allowing the reaction solution obtained in step (3) to stand at 40-60 °C to form a hydrogel film. This hydrogel not only has excellent mechanical properties, water absorption and retention properties, antioxidant properties, and swelling properties, but also can regulate drug release through pH response, and has the potential to be used as a drug carrier or wound dressing.

This invention belongs to the field of peanut cultivation and nitrogen use efficiency regulation technology. This invention provides a method for improving the nitrogen use efficiency of peanuts, comprising the following steps: applying a mixture of Bacillus preparation and water, along with a polyglutamic acid solution, to the rhizosphere via root irrigation during the initial flowering stage, and spraying a first composition onto the peanut leaves; spraying a second composition onto the peanut leaves during the full flowering stage; and spraying a third composition onto the peanut leaves during the pod-filling stage; and specifying the composition of the first, second, and third compositions. This invention improves the nitrogen use efficiency of peanuts by regulating the peanut rhizosphere environment and foliar nutrient supply in stages, promoting rhizosphere microbial activity, increasing the level of endogenous hormones in the roots, and enhancing the plant's ability to absorb, translocate, and distribute nitrogen. This invention has a clear regulatory approach, a complete technical route, and is applicable to the production of different peanut varieties.

The present application relates to the field of cosmetic technology, and discloses a preparation process of cashmere goat bone-derived elastin and application thereof in cosmetics. The process comprises: defatting cashmere goat bone particles; using composite rumen microbial flora to perform bionic fermentation to destruct bone matrix and expose active sites; adopting neutral protease and specific elastase to perform stepwise directional enzymolysis; performing hydrophilic modification through transglutaminase catalysis of covalent anchoring of polyglutamic acid and elastin polypeptide; and finally, performing multi-stage membrane interception purification and drying. The present application realizes complete retention of the natural cross-linking structure of elastin under mild conditions, significantly improves water solubility, biological activity and transdermal permeability of the elastin, solves the problems of structural damage and pollution caused by traditional chemical methods, and provides a green path for high-value utilization of cashmere goat by-products.

This invention provides a composite biosynergist, its preparation method, and its application. The composite biosynergist is composed of polyglutamic acid, humic acid, fucoidan oligosaccharide, and plant polyphenols. The synergistic effect of the active substances polyglutamic acid γ-PGA, humic acid HA, fucoidan oligosaccharide AOS, and plant polyphenol PP in the composite biosynergist is essentially manifested in microscopic-scale functional complementarity and mechanism closed-loop. It achieves four functional pathways: blocking salt toxicity through chelate channels, ensuring nutrient supply through complexation channels, enhancing nutrient absorption through root-promoting channels, and resisting stress damage through stress-resistant channels. Ultimately, it achieves the macroscopic effect of "improved soil physicochemical properties—enhanced root function—increased crop stress resistance and yield." Its core advantage lies in overcoming the functional limitations of single components and solving the industry pain points of "difficult-to-eliminate salt damage, easy nutrient loss, and unstable improvement effect" in saline-alkali land improvement through molecular-level synergistic effects.

The application provides a water-retaining soil conditioner containing high-efficiency degrading bacteria and a preparation method and application thereof, and belongs to the technical field of soil improvement.The water-retaining soil conditioner comprises a carboxymethylated modified humic acid-chitosan compound, modified attapulgite powder, a composite microbial inoculum, konjac glucosan, polyacrylic acid, bentonite, polyglutamic acid, sodium alginate, mushroom residue, seaweed residue and straw compost; the composite microbial inoculum is obtained by combination of Candida tropicalis, Bacillus smithii and Bacillus pasteurii.The application realizes precise adaptation of the carrier and the high-efficiency degrading bacterial group by optimizing the design of the composite water-retaining carrier and introducing specific bacterial combinations, strengthens the synergistic effect of water retention, bacteria fixation, slow release and soil improvement, realizes water retention, degradation and fertilization synergistic effect, and has important practical significance and application value for improving soil quality, guaranteeing crop production and promoting the development of ecological agriculture.

This invention relates to the field of saline-alkali land amendment technology. The invention provides a saline-alkali land amendment, its preparation method, and its application. The saline-alkali land amendment comprises the following raw materials in parts by weight: 50-60 parts of straw and livestock manure compost, 30-40 parts of lignite powder, 2-4 parts of desulfurized gypsum, 2-4 parts of citric acid gypsum, 3-4 parts of superphosphate, 3-4 parts of γ-polyglutamic acid, and 1-2 parts of a compound microbial agent solution. This invention effectively increases the organic carbon content of saline-alkali land and improves soil aggregate structure by introducing manure-based and mineral-based organic fertilizers; it enhances the calcium-increasing and sodium-removing effects of desulfurized gypsum, citric acid gypsum, and superphosphate; it introduces γ-polyglutamic acid, which has strong water retention and ion chelating capabilities, effectively fixing salts; and it introduces a compound microbial agent that not only regulates the microbial community composition of saline-alkali land but also produces acid to regulate soil pH and activate fixed phosphorus in the soil.

The application provides a multifunctional soil conditioner for improving the continuous cropping obstacles of yam and a preparation method thereof, the multifunctional soil conditioner comprises a nutrient component, an intelligent responsive component, an environmental regulation component and an additive, the mass ratio of the nutrient component, the intelligent responsive component, the environmental regulation component and the additive is 4-5:1:1-2:1-1.5, the nutrient component is earthworm compost, potassium humate and modified zeolite, the intelligent responsive component comprises mesoporous silica, ferulic acid and chitin oligosaccharide, the environmental regulation component comprises electron beam irradiation modified potassium polyacrylate, polyglutamic acid and polyacrylamide, and the additive comprises calcined diatomite and calcium carbonate, the soil conditioner provided by the application is not only rich in nutrients, but also has good water retention performance, and can well improve the continuous cropping obstacles of yam.

This invention discloses an integrated nanorobot system for medical aesthetics, belonging to the field of minimally invasive and non-invasive diagnostic and treatment technology in civilian medical aesthetics. The system uses glucose-modified PCL with a glucose grafting rate of 20-25 wt% as its core substrate, adaptable to a particle size range of 100-200 nm (median 150 nm). It is a non-disassembly integrated structure with a γ-polyglutamic acid modification layer on the main surface. It embeds six functional units: precise skin detection, precise drug delivery, minimally invasive ablation, repair and regeneration, pore cleansing, and safety regulation. Each unit communicates with the safety regulation unit via carbon nanotube nano-conduction links. The safety regulation unit and the γ-polyglutamic acid modification layer work together through specific logic to construct a pH dual-redundancy control logic specific to medical aesthetic scenarios. The false trigger rate on normal skin is ≤0.05%, cytotoxicity and mucosal irritation are both grade 0, and the degradation cycle is 14±2 days. The system has a mature and reproducible manufacturing process, with a yield rate of ≥93% in 100g-level small-scale preparation. All performance indicators meet the authoritative standards of FDA / ISO medical aesthetic devices. It is specifically designed for superficial medical aesthetic repair of the human epidermis / mucous membranes, solving the technical pain points of traditional medical aesthetic devices such as single function, low precision, and many complications. It realizes a one-stop closed-loop operation of "detection-treatment-repair" and has strong clinical applicability.

This invention discloses a microbial compound fertilizer for increasing perilla yield and its application, belonging to the field of microbial fertilizer technology. The microbial compound fertilizer comprises the following raw materials: compound microbial inoculant, humic acid, organic matter, trace elements, polyglutamic acid, and trehalose; the compound microbial inoculant includes *Bacillus polymyxa*, *Candida*, and *Penicillium oxalate*. This invention provides a microbial compound fertilizer that can significantly increase perilla yield. This microbial compound fertilizer integrates active ingredients such as organic matter, trace elements, and microorganisms. Through the scientific and rational compounding of these components, a stable synergistic effect is established among them, improving the soil environment and increasing fertilizer utilization, thereby effectively increasing perilla yield. It solves the problems of unstable microbial activity, insufficient fertilizer effect sustainability, and limited soil improvement effect in existing perilla cultivation, providing reliable technical support for high-quality and high-yield perilla cultivation.

This invention belongs to the field of soil conditioner technology, and relates to an organic-inorganic coupled conditioner for improving soda saline-alkali land and its preparation method. The inorganic particles are composed of a mixture of activated polyaluminum ferric sulfate, activated zeolite powder, transcrystalline gypsum, trace elements, and reinforcing agents. The organic slurry is homogenized from activated humic acid extract, modified lignin sulfonate, functional biochar complex, and polyglutamic acid concentrate, and then subjected to spray granulation and high-temperature, high-humidity aging to achieve chemical cross-linking at the organic-inorganic interface, forming a stable three-dimensional network structure. The resulting conditioner integrates chemical exchange, physical adsorption, organic complexation, and biostimulation, and can rapidly reduce soil pH and alkalinity, improve soil aggregate structure, enhance nutrient retention capacity, and has a long-lasting effect and is environmentally friendly, making it suitable for the comprehensive management of soda saline-alkali land.

This document discloses compositions comprising one or more polyglutamic acid compounds. These compositions are used to treat, improve, prevent, or alleviate symptoms of vaginal dryness and menopausal symptoms. This document also describes compositions for treating, improving, preventing, or alleviating other dry skin symptoms on the body, extremities, hands, feet, neck, or face in subjects of all ages and sexes. This document also describes topical and suppository formulations of the compositions.

This invention provides a polyglutamic acid-modified magnetic water-retaining agent and its room-temperature preparation method, belonging to the field of water-retaining agent technology. This invention achieves stable linking of magnetic particles and promotes cross-linking reactions through the introduction of polyglutamic acid, completing polymerization at room temperature, reducing production energy consumption, and improving product structural stability and water retention performance. Simultaneously, polyglutamic acid is a biodegradable and environmentally friendly raw material, enhancing the product's environmental friendliness.

The present invention provides a polymer material that exhibits sufficient shape memory while maintaining the advantages of hydrogels (flexibility, transparency, material encapsulation, biocompatibility, etc.), and is also biodegradable. [Solution] A shape-memory hydrogel containing a polymer matrix and water. The polymer matrix is ​​a biodegradable polymer having carboxyl groups, some of which are modified with glycinamide, and the carboxyl groups that are not modified with glycinamide are crosslinked with a crosslinking agent. An example of a biodegradable polymer having carboxyl groups is polyglutamic acid. An example of a crosslinking agent is ethylenediamine. Because the hydrogel of the present invention retains biodegradability, it is extremely safe and does not burden the environment when disposed of. Furthermore, it has extremely high hydrophilicity and can absorb and retain a large amount of water.

The present application provides a kind of to improve the nutrition fertilizer of yam stress resistance and its preparation and use method, the nutrition fertilizer includes base fertilizer and conditioning agent, the base fertilizer includes jasmonic acid-chitosan oligosaccharide conjugate, amino mesoporous silica nanoparticles, basic nutrient component, auxiliary material, binder, the basic nutrient component includes slow-release nitrogen source, readily available nitrogen source, phosphorus source, potassium source, trace element;The conditioning agent includes modified attapulgite, gamma-polyglutamic acid, sodium dodecyl sulfate.The nutrition fertilizer provided by the present application is not only comprehensive nutrition, and yam stress resistance can be effectively improved, and then the yield and quality of yam are improved.