265 results about "Clubroot" patented technology

The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony is light yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum; (2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.

This disclosure concerns a plant of the genus, Brassica, or parts thereof, which comprise one or more traits selected from the group consisting of high oleic acid content, low linolenic acid content, increased herbicide resistance, restorer of cytoplasmic male sterility, and increased clubroot disease (Plasmodiophora brassicae) resistance, compared to a wild-type plant of the same species. This disclosure further relates to wild-type and mutant alleles of genes involved in these traits, molecular markers linked thereto, and methods of their use.

The invention relates to bacillus subtilis M3 and an application thereof, and belongs to the technical field of biological pesticides. The produced strain is the bacillus subtilis M3; the preservation unit is the China General Microbiological Culture Collection Center; the address is Zhongguancun, Beijing, China; the preservation date is July 11, 2013; the preservation number is CGMCC NO.7911. The application of the bacillus subtilis M3 as a preparation for preventing and controlling Chinese cabbage clubroot, tomato scab, tomato root knot nematode diseases and konjak bacterial soft rot is also disclosed. The bacillus subtilis M3 disclosed by the invention achieves an obvious and stable prevention and control effect on the diseases, can be used for preparing the preparation for preventing and controlling the Chinese cabbage clubroot, the tomato scab, the tomato root knot nematode diseases and the konjak bacterial soft rot and has the advantages of high efficiency, nontoxicity, safety, no residue, bacteria activation, disease control, better growth promotion effect, better comprehensive character and easiness for industrialized production.

The invention discloses an InDel molecular marker for identifying clubroot-resistant QTL (quantitative trait locus) located on Chinese cabbage A03 chromosome and application thereof. The invention provides a method for identifying or auxiliarily identifying whether a Chinese cabbage is a clubroot-resistant Chinese cabbage, which comprises the following steps: carrying out amplification by using genome DNA (deoxyribonucleic acid) of the Chinese cabbage to be identified as a template, a single-stranded DNA disclosed as SEQ ID No.2 as a forward primer and a single-stranded DNA disclosed as SEQ ID No.3 as a reverse primer; detecting the size of the amplification product; and determining whether the Chinese cabbage to be identified is a clubroot-resistant Chinese cabbage according to the size of the amplification product: if the amplification product of the Chinese cabbage to be identified contains a 201bp strip, the Chinese cabbage to be identified is a clubroot-resistant Chinese cabbage or candidate clubroot-resistant Chinese cabbage; and if the amplification product of the Chinese cabbage to be identified does not contain any 201bp strip, the Chinese cabbage to be identified is a non-clubroot-resistant Chinese cabbage or non-candidate clubroot-resistant Chinese cabbage.

The invention discloses a specific SNP (Single Nucleotide Polymorphism) molecular marker for identifying cabbage clubroot 4# physiological race resistance and application. The invention provides application of an A0220509373G/T site in any one aspect as follows: A, application to identification or auxiliary identification of cabbage clubroot resistance; B, application to breeding of cabbage-clubroot-resistant varieties; C, application to cabbage breeding; D, application to prediction of cabbage clubroot resistance; E, application to preparing a product for identification or auxiliary identification of cabbage clubroot resistance; and F, application to preparation of a product for predicting cabbage clubroot resistance. Experiments prove that the A0220509373G/T site and a specific primer of the A0220509373G/T site can realize good typing on the cabbage clubroot; a good application value is realized; and the selection in advance and the auxiliary breeding on materials resisting the clubroot inheritance can be realized.

Successfully produced are cruciferous plants resistant to clubroot by introducing the clubroot resistance gene (Crr1) isolated by map-based cloning into cruciferous plants and expressing the gene.

The invention discloses a soil adjuster to prevent Cruciferae crop from clubroot, which comprises the following parts: 10-50% organics, 10-60% calcium magnesium phosphate fertilizer, 15-80% carbonate, 0. 8-5% potassium, 0. 8-5% magnesium, 0. 8-5% zinc, 0. 8-5% boron, wherein the wild or cultivated organics adopts Tagetes plant as raw material; the wild plant can be harvested during blossom period, which is dried and grinded into powder. The invention can avoid the clubroot to 80-95%, which is economic, safe, high-effective and convenient.

The invention discloses a molecular marker of a brassica napus anti-clubroot gene PbBa8.1 and a method for applying the molecular marker to anti-clubroot breeding through re-sequencing. The DNA molecular marker, capable of distinguishing anti-clubroot brassica napus and brassica napus being not resistant to clubroot, is obtained by conducting polymorphic analysis on sequences, around a PbBa8.1 locus, of two parents, and carrying out primer development design by utilizing the flanking sequences of an Indel locus. Primers, namely F A08-300: 5'-GTAGTGCGGGCCACAAAAT-3' and R A08-300: 5'-CACAATGGAGTGTTGAAATTCACT-3', aimed at the molecular marker are designed, and can be used for improving the anti-clubroot gene breeding speed and identification efficiency. The method is easy to carry out, high in repeatability, operability and identification speed, and low in detection cost, time consumption and labor cost, and has the advantages that the workload of selecting an anti-clubroot singleplant through phenotype identification in a field is greatly reduced.

The invention relates to a Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 and an application, which belongs to the biology technical field. The Pantoea agglomerans1-7 is preserved in the China General Microbiological Culture Collection Center on May 28th, 2012 with the preservation number of CGMCC No.6160. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 is used for preparing the preparations used for preventing and treating bacterial soft rot of konjak and clubroot disease on cruciferous vegetable club root in fields. The Konjak endophytic bacteria Pantoea agglomerans bacterial strain1-7 has the advantages of high efficiency, no toxicity, safety and no residue, the bacterial strain1-7 has the characteristics of good disease control, better growth promotion effect, better comprehensive characters and easy industrial production, and the Pantoea agglomerans1-7 can be applied to preparation of preparations for controlling cruciferous vegetable club root in fields.

The invention discloses long-acting biological medicine fertilizer for peanuts and a preparation method of the long-acting biological medicine fertilizer. The long-acting biological medicine fertilizer is prepared from, by weight, 0.5-1 part of paenibacillus polymyxa, 1-10 parts of chlorpyrifos-carbosulfan suspension microcapsules, 25-35 parts of urea, 30-40 parts of monoammonium phosphate and 30-40 parts of potassium sulphate, and the number of viable bacteria of the paenibacillus polymyxa is 900-1100 million cfu/g. The medicine fertilizer can keep the pesticide effect during the whole processes of sowing of the peanuts and hardening of peanut shells, can conduct insect prevention and can prevent bacterial wilt, blight, root rot, clubroot and other soil-borne diseases, the yield is raised, and the whole course of control over prevention of diseases and insect pests is achieved.

The invention belongs to the technical field of agricultural sustainable development, relates to preparation of organic preparations and in particular relates to an organic preparation for controllingcruciferous vegetable club root and application thereof. The organic preparation is composed of organic raw materials and decomposition-promoting ingredients, wherein the organic materials include but not limited to one or more of crop straws, saw dust and other solid organic materials and molasses, alcoholic fermentation liquor and other liquid organic materials; the decomposition-promoting ingredients include but not limited to one or more of special microbes and organic catalysts; and a ratio (mass) of the organic raw materials to the decomposition-promoting ingredients is (95-100):(5-0).The invention further discloses a preparation method and application of the organic preparation. The organic preparation disclosed by the invention is ecologically environment-friendly and is applicable to soil with high incidence of club root after continuous cropping of various cruciferous vegetables. The quantity of plasmodiophora brassicae woron in soil and the incidence rate of the club rootof crops can be effectively reduced after treatment, the sterilization rate and the club root control rate respectively reach 60-90%, and the yield of the crops is obviously increased.

The invention discloses an identification method for the clubroot resistance of alpine radish at the seedling stage. The identification method is characterized by comprising the following steps: S1, sieving soil of cultivated land, adding with a fertilizer and plant ash, mixing sterile soil with an organic fertilizer and calcium superphosphate, in addition, adding with plant ash, and preparing culture soil; S2, taking a hole tray as a culture device, adding the culture soil obtained in S1 into the hole tray, and sowing the seeds with the variety to be identified in the hole tray; S3, grinding fungus roots containing plasmodiophoromycetes, filtering the grinded fungus roots by adopting gauze, preparing a pathogenic spore solution, carrying out counting by adopting a blood counting chamber, preparing the spore solution into inoculating bacteria liquid with the concentration being 1x10<7> spores/ml for later use; S4, adding the culture soil into the hole tray for later use; and S5, completely watering the culture soil in the hole tray, when the water settles and the soil is slightly dry and is suitable for sowing, carrying out dibble seeding and earthing at the central point of each hole, establishing a small shed, carrying out normal field management, and investigating the morbidity at the 7th week after inoculation. According to the identification method provided by the invention, through the root irrigation identification method, the clubroot resistance of relevant experimental population can be improved, and thus the method has great values for the disease-resisting breeding practice and theoretical research.

The invention discloses an identification method for clubroot resistance of non-heading cabbages. The identification method comprises the following steps: preparing a clubroot bacterium suspension with the concentration of 1*10<7> to 2*10<8>bacteria/ mL; when seedlings of the non-heading cabbage grow to have three leaves and one heart, uniformly irrigating the roots of the seedlings with the prepared clubroot bacterium suspension so as to soak the roots of the seedlings in the clubroot bacterium suspension for 3-4 days; taking the seedlings out of the clubroot bacterium suspension, culturing the seedlings for 40-50 days by a conventional method, and identifying and screening non-heading cabbages which are not infected by clubroot. By the identification method, when the seedlings of the non-heading cabbages grow to have three leaves and one heart, the seedlings are inoculated with clubroot bacteria by a root dipping method; by investigating the illness occurrence situation of plants, the disease-resistant or infected non-heading cabbages are identified.

The invention discloses an identification method for clubroot disease resistance of high mountain radishes in a field. The identification method comprises the following steps: selecting experimental radish varieties, enriching bacterium sources of a clubroot disease nursery in a high mountain region, and sowing radishes; grading clubroot disease morbidity degree of full-grown radishes to obtain disease condition grades of the radishes; calculating radish variety disease condition indexes according to the disease condition grades of the radishes and a radish variety disease condition index general formula; and evaluating the resistance type of each radish variety to the clubroot disease by combining a radish disease resistance evaluation standard according to a calculation result. According to the identification method, the disease condition grading of the radishes which are infected with a disease is simplified; the influence on radish clubroot disease condition grading caused by black spots and black scars on the radishes is excluded; the existing grading mode is changed essentially; the overall identification process and the result processing analysis become simple, and meanwhile, the clubroot disease resistance of different radish varieties is judged rigorously and accurately.

The invention relates to an integrated control method for cruciferous vegetable clubroot, which comprises the following steps: 1. ecological treatment: a. applying HYT, namely applying HYT-A 200-250ml, HYT-B 250-350ml and HYT-C 100-120g every Mu, and operate the procedure 2-4 times during the whole growing period; b. applying traditional Chinese medicine microscale fertilizer one month before the cruciferous vegetable ripe, and apply the traditional Chinese medicine microscale fertilizer 20-25g every Mu; 2. agriculture control; 3. plant quarantine; 4. biological control; 5. physical control; and 6. chemical control. The method comprises the steps of ecological treatment, agriculture control, plant quarantine, physical control chemical control, therefore, the incidence of disease of the cruciferous vegetable clubroot is greatly reduced; the effect is good; and the output and the quality of the cruciferous vegetable is improved.

The invention provides a soil conditioner for preventing and treating clubroot diseases of cruciferae crops and application of the soil conditioner. The soil conditioner comprises, by weight, 70-80 parts of water-soluble calcium, 0.1-1 part of water-soluble magnesium, 4-5 parts of organic nitrogen, 8-15 parts of humus, 6-15 parts of pellet binders and 20-25 parts of sustained-release preparations. The invention further provides a preparation method of the soil conditioner. The soil conditioner can have a good effect on preventing and treating the clubroot diseases of the cruciferae crops and solve the problem of the clubroot diseases of the cruciferae crops fundamentally; the soil conditioner is free of environmental pollution, beneficial to environmental protection and food safety, safe and convenient to use, particularly suitable for the rice planting season of a paddy-upland rotation region, capable of achieving the double functions of slowly and evenly up-regulating soil acidity and alkalinity and providing nitrogenous fertilizer required by rice in the green-turning period, and good in application and popularization prospect.

The invention discloses a method for evaluating resistance of brassica plants on club root, which belongs to the brassica plant disease resistance evaluation technical field, and is characterized in that: perlite after being soaked by acid water is mixed with crushed brassica plasmodiophora brassicae fungus root according to a ratio of 1L: 10 to 15g to form culture matrix, the culture matrix is filled inside grids of a frozen box, seeds of a specimen to be evaluated and infected specimen seeds infecting the club root are planted inside the grids of the frozen box, one seed is planted inside each grid, the frozen box is arranged inside a ceramic dish, and 1/2MS culture matrix and the crushed brassica plasmodiophora brassicae fungus root are used for being made into inoculant liquid with pH value of 5.5 and content of pathogenic bacteria spores of 1*108/ml; and the inoculant liquid is filled into the ceramic dish to be cultured inside an artificial weather box, and the resistance of the specimen to be evaluated on the club root is evaluated according to the investigated disease indexes. Due to the adoption of the method, mass evaluation of the resistance on the club root can be realized, a great amount of land can be saved, consistence of the evaluation conditions can be guaranteed, time and labor can be saved, the evaluation result is accurate and reliable, and simplicity and convenience in operation can be realized.

The invention discloses a method for preventing and treating celery cabbage clubroot. The method includes the following steps of 1) species selection and seed pretreatment, 2) sowing, 3) large field soil preparation and fertilization, 4) hole excavation and application of organic bacterial manure and basic converter steelmaking slag mixture, 5) cabbage seedling planting and 6) field management including 6.1) additional fertilizer application and 6.2) pest control. By applying the organic bacterial manure in a large field, pathogenic bacteria can be removed, and beneficial bacteria are multiplied. Micro elements of the basic converter steelmaking slag with M0 larger than 1 are utilized to improve acid soil and prevent and treat the celery cabbage clubroot, application quantity of chemical pesticide can be reduced, and the waste converter steelmaking slag can be turned into wealth. The method is a harmless celery cabbage clubroot preventing and treating technology. By means of the method, celery cabbage yield and quality are improved, and remarkable economical, social and ecological benefits can be obtained.

The invention discloses a seedling substrate for preventing and controlling cabbage clubroot, and belongs to the technical field of vegetable culture. The seedling substrate is obtained by the steps: a, measuring organic substrates (humic soil or turf or coco coir or bagasse or wood dust), cow dung and plant ash according to the volume ratio of 8:2:1 and pouring the organic substrates, the cow dung and the plant ash into a stirrer/stirring machine; b, grinding weighed 2.1-2.5kg/m<3> of urea and 1.5-1.8kg/m<3> of ammonium dihydrogen phosphate into powder, adding the powder along with 1.5-1.8kg/m<3> of calcined lime into the stirrer/stirring machine; c, adding 1.2-1.5kg/m<3> of wettable powder with 75% of chlorothalonil into the stirrer/stirring machine; and d, starting the machine, uniformly stirring the powder and sub-packaging the powder. The seedling substrate has the advantages that vegetable shoots are fine in quality, the clubroot is avoided, diseases are relieved after planting, control effect is long, the seedling substrate is not limited by physiological race, and environmental side effects are avoided.

The invention discloses a rapeseed endogenous trichoderma atroviride ReTv2 strain and a preparation method and application thereof. The trichoderma atroviride ReTv2 strain is separated from a rapeseed root tissue by the applicant, and the number CCTCC NO is M2014407. The ReTv2 strain is activated and cultured in a PDA culture medium at the temperature of 28 DEG C for 2-4d to obtain conidiospores, and the spores are inoculated into a PDB culture medium for enlarged culture for 6d, wherein the content of the conidiospores can achieve 1.0*10<9>/mL. The trichoderma atroviride ReTv2 strain disclosed by the invention is the rapeseed endogenous strain, can be successfully colonized at the root of rapeseed and has an effect on promoting growth of the rapeseed, a fermentation broth has an obvious effect on inhibiting germination of resting spores of pathogenic bacteria of rapeseed clubroot, and the control effect of the strain against the rapeseed clubroot achieves 66.4%; furthermore, the ReTv2 strain can be directly applied during seeding, is simple in an application method, can save labor force and further has the property of being safe to people and animals of biological pesticides.

The invention discloses a method for obtaining germplasm resources of non-heading Chinese cabbage resistant to clubroot, comprising: selecting introduced Chinese cabbage resistant to clubroot and susceptible non-heading Chinese cabbage with excellent agronomic properties; Interspecific hybridization was used to obtain F1 seeds of the first generation of hybridization; the screened F1 generation was continuously backcrossed for 4 generations, and a new non-heading Chinese cabbage resistant germplasm Q15‑1 with stable phenotype and resistance to clubroot was initially obtained. The present invention adopts the method of interspecific hybridization, and carries out artificial inoculation identification on each backcross offspring, screens the resistant single plant of clubroot disease, and finally obtains new germplasm. The present invention utilizes Brassicaceae Chinese cabbage The hybrid vigor between the two varieties of non-heading Chinese cabbage and its variant non-heading Chinese cabbage, the resistance gene of clubroot Chinese cabbage is transferred to non-heading Chinese cabbage through hybridization and backcrossing at the bud stage, which is simple and easy. Biological risks caused by other methods such as genetic modification and genetic engineering technology are excluded.

The invention discloses a specific PCR detection method for plasmodiophora brassicae in soil. The invention is characterized in that primers are designed as shown in SEQ ID No 1,2,3,5 according to 1-1028 sequences in a SEQ ID No 10 to form primer pairs 2,4,5 for performing the specific PCR detection. The method of the invention has the advantages of rapid detection, sensitive method, good specificity, wide application and simple operation, and can be taken as an effective technology means for detecting brassica plasmodiophora brassicae in soil and plant organization which has high practical value.

The invention provides an application method of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae. The method comprises the following two methods: the method I comprises: carrying out root-irrigation treatment on a cruciferae crop by a bacillus amyloliquefaciens Bamm22 bacterial liquid with the amount of 80-100 ml per time per strain, and carrying out treatment for at least three times, wherein the treatment times are the first day, the seventh day and the fifteenth day after transplanting; the method II comprises: carrying out spraying treatment on the cruciferae crop by a bacillus amyloliquefaciens Bamm22 bacterial liquid with the amount of 150-200 ml per square meter, and carrying out treatment for at least three times, wherein the treatment times are the first day, the seventh day and the fourteenth day after seeding. The bacillus amyloliquefaciens Bamm22 provided by the invention is simple in using method, the application concentration of the bacterial liquid is less and the application dosage of the bacterial liquid is less.