10 results about "Clubroot" patented technology

The application belongs to the technical field of genetic engineering, and particularly relates to application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases. The application provides application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases, and the amino acid sequence of the AtPEN2 protein is shown as SEQ ID NO. 1. The application takes Arabidopsis thaliana as a model plant to verify the prevention and treatment effect of overexpression of AtPEN2 protein on clubroot, and the results show that the AtPEN2 protein overexpression plant can improve the resistance to clubroot, indicating that the coding gene AtPEN2 of the AtPEN2 protein can be applied to molecular breeding of clubroot, and provides a new gene resource for molecular breeding of cruciferous crops.

The present application relates to the field of agricultural biotechnology and plant disease control technology, and specifically discloses a composition for preventing and controlling rape root rot, a preparation method and application thereof. The composition is prepared by fermentation of the following components in parts by weight: tea seed cake 10-30 parts, oyster shell powder 20-30 parts, waste molasses 10-20 parts, brewer's yeast powder 1-5 parts, xylo-oligosaccharide industrial residue 1-3 parts, and a composite fermentation agent containing lactobacillus plantarum, bacillus coagulans and propionic acid-producing propionibacterium 1-3 parts. The fermentation process adopts a two-stage pH control timing inoculation process: in the first stage, brewer's yeast is inoculated; in the second stage, the composite fermentation agent is inoculated under acidic conditions, and through synergistic metabolism, the sugar source is efficiently converted into a short-chain fatty acid composite system mainly composed of lactic acid and propionic acid. The prevention and control effect on rape root rot is significantly improved, and the composition also has the functions of improving soil and promoting crop growth, and has the advantages of high efficiency, environmental protection and multi-effect combination.

The application belongs to the field of agricultural biotechnology, and particularly relates to application of a calmodulin-binding protein CBP60g or a coding gene CBP60g thereof in control of clubroot, wherein an amino acid sequence of the calmodulin-binding protein CBP60g is shown as SEQ ID NO. 1. The application can restore the resistance of Arabidopsis thaliana to Plasmodiophora brassicae at high temperature by overexpressing CBP60g, and the same is verified in Brassica napus, and the normal growth, yield and quality of the Brassica napus are not affected, which indicates that the CBP60g gene can resist Plasmodiophora brassicae at high temperature, and increase the recovery of the immune response of crops to high-temperature combined stress, and provides a strategy for molecular breeding in response to climate change.

The application discloses application of NPR1 protein and related biological materials thereof in regulating resistance of plants to clubroot disease, and belongs to the technical field of genetic engineering. The technical problem to be solved by the application is how to regulate resistance of plants to Plasmodiophora brassicae. Specifically, the technical problem to be solved by the application is how to regulate resistance of cruciferous plants to Plasmodiophora brassicae. To solve the above technical problem, the application provides application of NPR1 protein or biological materials related to the NPR1 protein in regulating resistance of plants to clubroot disease. The application discloses, for the first time, application of NPR1 protein and a coding gene thereof in regulating resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae. Research on the function of NPR1 and application of the function in cruciferous plant breeding are of great significance to the mechanism of resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae and to resistance breeding.

This application discloses the application of the EDS1 protein and its encoding gene in regulating plant disease resistance, belonging to the field of genetic engineering technology. The technical problem this application aims to solve is: how to regulate clubroot resistance in plants. To this end, this application provides the application of the EDS1 protein, or substances regulating the expression of the EDS1 protein encoding gene, or substances regulating the content of the EDS1 protein, in regulating plant clubroot resistance; the EDS1 protein can be a protein with the amino acid sequence being sequence 2. This application is the first confirmation of EDS1's participation in the *Cladophora brassicae* infection cycle, and the obtained susceptible materials can be utilized as a potential breeding resource. The discovery and enrichment of breeding resources will greatly accelerate the breeding process of clubroot-resistant cruciferous plants and strengthen disease control. Furthermore, clubroot resistance can be achieved by overexpressing EDS1 in cruciferous plants through transgenic or other means.

This invention discloses a method for the propagation and renewal of *Avicennia marina* germplasm resources. *Avicennia marina* is grafted onto radish rootstock to achieve propagation and renewal. This one-step tissue culture regeneration method eliminates the need for explant cutting, complex culture media, the addition of exogenous hormones, and multiple culture media changes, saving manpower and financial resources and preventing contamination risks during culture media changes. Compared with existing technologies, this root-transplantation cultivation method effectively solves the problem of *Avicennia marina* roots being unsuitable for soil, leading to early plant death and hindering propagation and renewal. Furthermore, utilizing radish roots, which are highly resistant to clubroot disease, solves the problem of clubroot disease and even plant death. In addition, radish roots can be harvested and stored for overwintering, maintaining a very high survival rate the following year. Compared to other cruciferous crops such as cabbage and mustard, using radish as a root donor facilitates overwintering and the subsequent greening and reproductive growth of *Avicennia marina* the following year.

This invention discloses a synbiotic composition for preventing and controlling clubroot disease in Chinese cabbage, its preparation method, and its application, relating to the field of agricultural biotechnology. The synbiotic composition comprises carbon quantum dots, flavonoid compounds, and Bacillus subtilis (…). Bacillus subtilis The ratio of the carbon quantum dots, the flavonoid compounds, and the Bacillus subtilis is (0.1-5) g: (0.5-20) g: (10 g). 11 -10 12 CFU. This invention solves the technical problems of unstable efficacy and imprecise rhizosphere microecological regulation of existing biocontrol agents. It deeply integrates physical (carbon quantum dots), chemical (flavonoids), and biological (biocontrol bacteria) methods to achieve a synergistic control effect of "1+1+1>3" for clubroot disease in Chinese cabbage.

The invention relates to a method for preventing or reducing plant gall formation, the method comprising applying on a plant a composition comprising a suitable amount of at least one ingredient selected from the group consisting of cytokinins, auxins, or auxin precursors. The auxin is preferably indole-3-butyric acid (IBA). The invention further relates to a method for treating seeds. The invention further relates to the use of a composition comprising a suitable amount of an auxin or an auxin precursor, for the prevention and / or reduction of plant gall formation. The gall formation is preferably caused by clubroot disease.

The application belongs to the technical field of genetic engineering, and relates to a kind of resistance to clubroot cabbage gene Bol.TNL.2 and its application, the nucleotide sequence of resistance to clubroot cabbage gene Bol.TNL.2 is SEQ ID No.1, and gene is located on C07 chromosome.The application is analyzed to the BC1F1 population constructed by resistance cabbage Z5 and susceptible cabbage WG12 by BSA method, target gene is located on C07 chromosome, resistance to clubroot cabbage gene Bol.TNL.2 with TNL structure is found, new clubroot resistance site is developed, and new variety of plant with broad-spectrum resistance to clubroot is bred using gene Bol.TNL.2, to meet actual demand.

The invention provides low erucic, clubroot resistant Brassica plants, plant material and seeds, characterized in that these products harbor a specific CrS clubroot resistance locus in their genome. Tools are also provided which allow detection of the CrS clubroot resistance locus.