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InDel molecular marker for identifying clubroot-resistant QTL (quantitative trait locus) located on Chinese cabbage A03 chromosome and application thereof

A technology for Chinese cabbage clubroot and clubroot resistance, applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve problems such as unsatisfactory control effects, achieve accurate and reliable identification results, and speed up large-scale production. The progress of cabbage resistance to clubroot breeding and the effect of reducing labor costs

Inactive Publication Date: 2016-05-04
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although clubroot can be controlled by chemical agents and enhanced cultivation management, the control effect is not satisfactory

Method used

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  • InDel molecular marker for identifying clubroot-resistant QTL (quantitative trait locus) located on Chinese cabbage A03 chromosome and application thereof
  • InDel molecular marker for identifying clubroot-resistant QTL (quantitative trait locus) located on Chinese cabbage A03 chromosome and application thereof
  • InDel molecular marker for identifying clubroot-resistant QTL (quantitative trait locus) located on Chinese cabbage A03 chromosome and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Embodiment 1, the identification of physiological race of Plasmodium brassicae

[0051] The Williams identification system was used to identify the physiological races of Plasmodium brassicae collected from Yunnan Tonghai, Shenyang Xinmin, and Dalian Shuishiying Chinese cabbage production areas. The inoculation, investigation and physiological race identification methods refer to the literature "Wang Weihong et al. (2013) , Physiological race identification and resistance source screening of Brassicaceae clubroot bacteria in Changyang County, Hubei Province, Chinese vegetables". The results showed (Table 1): the pathogenic bacteria of Chinese cabbage clubroot from Tonghai, Yunnan Province was Physiological Race No. 7, and the pathogenic bacteria of Chinese cabbage clubroot from Shenyang Xinmin and Dalian Shuishiying was Plasmodium brassicae Race 2.

[0052] Table 1: Race identification results of Plasmodium brassicae from different sources

[0053]

[0054] Note: +...

Embodiment 2

[0055] Embodiment 2, Chinese cabbage clubroot disease-resistant parents and BC 2 f 2 group acquisition

[0056] 1. Acquisition of Chinese cabbage clubroot disease-resistant parent "CR-Weimin A"

[0057] The Chinese cabbage clubroot inbred line "CR-Weimin" was obtained by self-segregation of the Chinese cabbage hybrid "CR-Weimin" for 6 consecutive generations showing resistance to Plasmodium brassica races 2 and 7 A".

[0058] Two, BC 2 f 2 group acquisition

[0059] Chinese cabbage "CR-Weimin A" was used as the disease-resistant parent (female parent) and Chinese cabbage "Beijing Xin No. 3" was used as the susceptible parent (male parent) for hybridization, and the F1 generation seeds were harvested. Plant F1 generation seeds to obtain F1 generation Chinese cabbage plants, backcross the F1 generation Chinese cabbage with the susceptible parent "Beijing Xin 3", and harvest BC 2 f 2 group seeds.

Embodiment 3

[0060] Embodiment 3, BC 2 f 2 Phenotype identification and molecular marker verification of clubroot disease in Chinese cabbage

[0061] 1. BC 2 f 2 Phenotypic Identification of Clubroot Disease Resistance in Chinese Cabbage

[0062] With the BC obtained in Example 2 2 f 2 Populations were used as materials. After sowing, they were inoculated with Plasmodium brassicae from Tonghai, Shenyang, and Shuishiying, Dalian. The inoculation and investigation methods were referred to in the literature "Wang Weihong et al. (2013), Clubroot of Cruciferous Vegetables in Changyang County, Hubei Province Physiological race identification of pathogens and source of resistance screening, the method described in "Chinese Vegetables". Investigate the condition according to the 0, 1, 2, 3, 4 disease classification standards, and calculate the disease index (the disease index = ∑ (the representative value of the disease level × the number of diseased plants) / the total number of investigate...

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Abstract

The invention discloses an InDel molecular marker for identifying clubroot-resistant QTL (quantitative trait locus) located on Chinese cabbage A03 chromosome and application thereof. The invention provides a method for identifying or auxiliarily identifying whether a Chinese cabbage is a clubroot-resistant Chinese cabbage, which comprises the following steps: carrying out amplification by using genome DNA (deoxyribonucleic acid) of the Chinese cabbage to be identified as a template, a single-stranded DNA disclosed as SEQ ID No.2 as a forward primer and a single-stranded DNA disclosed as SEQ ID No.3 as a reverse primer; detecting the size of the amplification product; and determining whether the Chinese cabbage to be identified is a clubroot-resistant Chinese cabbage according to the size of the amplification product: if the amplification product of the Chinese cabbage to be identified contains a 201bp strip, the Chinese cabbage to be identified is a clubroot-resistant Chinese cabbage or candidate clubroot-resistant Chinese cabbage; and if the amplification product of the Chinese cabbage to be identified does not contain any 201bp strip, the Chinese cabbage to be identified is a non-clubroot-resistant Chinese cabbage or non-candidate clubroot-resistant Chinese cabbage.

Description

technical field [0001] The invention relates to a molecular marker for identifying Chinese cabbage clubroot disease resistance in the field of biotechnology and its application, in particular to an InDel molecular marker related to the resistance of Plasmodium brassicae No. 2 and No. 7 physiological races and its application. Background technique [0002] Cruciferous plant clubroot is a soil-borne fungal disease caused by the flagellate subphylum Plasmodiophora brassicae Woron, and it is an important root disease of Brassicaceae plants. The disease was first discovered in 1737 on the British Mediterranean west coast and southern Europe. At present, it has become a major disease in Europe, North America, Japan and other places in Asia, posing a serious threat to the production of cruciferous vegetables. It is distributed all over the world, especially in temperate regions. In recent years, cruciferous clubroot has rapidly expanded in Northeast China, Southwest China, the mid...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 汪维红于拴仓苏同兵张凤兰余阳俊张德双赵岫云卢桂香
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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