Method for rapidly identifying broccoli variety Tailv1 hybrid

Abstract

The invention provides a method for rapidly, accurately and efficiently identifying the molecules of a broccoli variety Tailv1 hybrid. The method comprises the following steps: extracting genomic DNA from a sample to be detected, carrying out PCR amplification on the genomic DNA by a PCR primer of 5'-TCTCTCTCTCTCTCTCN-3', carrying out gel electrophoresis imaging analysis on the obtained PCR amplification products, and judging the sample to be detected as a Tailv1 hybrid single plant if about 800bp and about 600bp brands are simultaneously obtained through amplification; and calculating according to a formula of genetic purity of hybrid = number of hybrid plants / number of sample plants to be detected *100% to obtain the genetic purity of the sample to be detected. The accuracy of the identification of the purity of the broccoli variety Tailv1 hybrid by an ISSR primer TNISSR15 used in the invention is high, has a 99% consistency with a field identification result, and is reliable; the identification can be completed within 10d, the tedious program of field planting identification is avoided, the labor force and the land resource are saved, and the identification cost is reduced; and the method can determine the genetic purity of seeds in the same year of seed production, is convenient for seed graduation and purchase, and improves the identification efficiency.

Description

(1) Technical field [0001] The invention relates to a rapid identification method for a broccoli variety 'Tailu No. 1' hybrid. (2) Background technology [0002] Broccoli (Brassica oleracea var. italica), also known as green cauliflower, broccoli, etc., is a variety of Brassica oleracea Brassica species. Its edible part is the fat and tender flower stem with flower buds, which is rich in nutrition, unique in flavor, and has anti-cancer and anti-oxidation effects, and is deeply loved by consumers. In recent years, the cultivated area of ​​broccoli in my country has increased year by year, and the demand for dominant broccoli varieties has also continued to increase. The use of heterosis to produce broccoli F 1 Hybrids have become a trend. However, in the process of hybrid seed production, the female parent self-crosses, the mutual pollination of people in the operation process, and the pollination caused by birds, bees, etc. will all form false hybrids. In addition, broccol...

AI Technical Summary

Problems solved by technology

Compared with SSR, ISSR is more versatile among different species and easier to develop primers. Compared with RAPD, it has higher stability. It is a simple and low-cost detection method. Currently, researchers have used ISSR The hybrid purity identification of rice, cucumber and other crops has been carried out, but there is no report on the application of ISSR to the genetic purity identification of broccoli hybrids

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