The present application belongs to the technical field of
plant induced regeneration, and particularly relates to a high-efficiency artificial propagation method of
Dendrobium candidum, comprising the following steps: taking two-year-old stem segments with nodes as materials,
cutting the stem segments into appropriate sizes, and disinfecting the stem segments; and culturing the stem segments in a culture medium A for 90 days, and then completing the proliferation and rooting integration through a direct
organogenesis route. The present application realizes the cyclic production of
Dendrobium candidum, and the whole culture process can be completed only by using one culture medium, greatly improving the artificial in-vitro propagation efficiency, and the proliferation coefficient is completely comparable to the seed asymbiotic
germination method, and the problems of large
offspring trait separation and different qualities caused by the seed asymbiotic
germination are solved, and the quality and yield of seedlings are greatly improved. The present application can provide a brand-new
technical support for the factory-scale growth of
Dendrobium candidum, so as to achieve the purpose of cultivating seedlings with
genetic stability and excellent quality to improve the artificial planting efficiency, and the cost is low, the cycle is short, and the quality and
survival rate of seedlings are high.