41 results about "Gametophyte" patented technology

The invention discloses a moss ecological brick and a preparation method thereof, and relates to the technical field of ecological restoration, the moss ecological brick comprises a moss gametophyte matrix and a brick body, the brick body comprises the following raw materials: a tailing composite material, metakaolin, an acid activator and water, and the tailing composite material comprises phosphate tailings and iron tailings. The phosphate tailings and the iron tailings can provide necessary phosphorus elements, other trace elements and the like for the growth of the moss, and the acid activator in the brick body not only can provide a weak acid environment to facilitate the good growth of the moss, but also can solve the problem of saltpetering of the brick body in the environment; the growth of the moss can effectively relieve sulfate erosion of the acid-activated material, and a good curing environment is provided for the acid-activated concrete brick body. Therefore, in the moss ecological brick provided by the invention, the acid-activated concrete brick body can supplement and promote the growth of moss.

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

Herein is described a method devised to cryopreserve mosquito eggs, viable upon subsequent thawing. The method reproducibly yields >25% hatched first instar larvae of Anopheles-species mosquitoes from cryopreserved eggs. The majority of hatched larvae continue to develop normally (87% pupation), through to adults (98%, 50% of which are female) which subsequently blood feed and produce viable second generation embryos that also develop normally. Adult mosquitoes obtained from cryopreserved embryos are able to be infected with Plasmodium falciparum gametocytes and produce salivary gland sporozoites in similar numbers to control mosquitoes.

The present invention relates to a Babesia antigen comprising at least a portion of a gametocyte HAPLESS2 / GCS1 (HAP2) protein, vectors and cells expressing such antigen, compositions and kits comprising such antigens, and methods of using such antigens to interfere with the Babesia transmission by competent ticks.

The invention discloses a kelp gametophyte gene editing method and application, and belongs to the technical field of gene editing, the kelp gametophyte gene editing method comprises the following steps: preparing a kelp gametophyte receptor; assembling an RNP complex and preparing a microcarrier; introducing the RNP compound into a kelp gametophyte by using a gene gun; and recovering culture and screening. According to the technical scheme, the dependency of a microinjection technology on precise instruments and professional operation skills is eliminated, efficient gene delivery can be achieved through a standardized gene gun operation process, the technical threshold is greatly reduced, and the kelp gametophyte gene editing method has remarkable technical advantages. According to the method, the high-throughput characteristic of a gene gun technology is fully exerted, multiple mutants can be obtained through one-time bombardment, the experiment efficiency is greatly improved, and powerful support is provided for kelp large-scale gene function research and molecular breeding.

This invention provides for the development of singularity-inducing substances and their use in generating singularity plants and in doubling singularity technology. [Solution] The present invention provides a plant comprising a polynucleic acid encoding a mutated undetermined gametophyte (ig) protein and a polynucleic acid encoding a mutated centromere or kinetochore protein. The mutated centromere or kinetochore protein is preferably CENH3. The mutated ig and the centromere or kinetochore protein together result in singular induction activity, for example, paternal singular induction activity. The present invention further provides a method for producing such a plant and its use.

The invention relates to a photobioreactor (1) for the submerged production of sphagnum moss, comprising a container (10) with a reactor interior (RI) comprising at least a portion of the reactor interior (RI). The container (10) has a first compartment (11) and a second compartment (12) arranged above the first compartment (11), wherein the compartments (11, 12, 13) form at least a portion of the reactor interior (RI), are substantially transparent, and have a height (H) to diameter (D) ratio of each compartment of 1 to 2.6. Furthermore, the container (10) has a separating element (14) arranged between the first and the second compartment (11, 12) having a plurality of openings (141) for fluid-conducting connection between the compartments (11, 12, 13) and a closable access opening for adding and removing mineral nutrient medium and peat moss gametophytes into or from at least part of the reactor interior (RI).Furthermore, the container (10) comprises a gas distributor (15) arranged centrally in a lower region (II) of the reactor interior (RI), with a plurality of gas openings for supplying gas into at least a part of the reactor interior (RI) along a gassed sub-region of a photobioreactor cross-sectional area, and a gas outlet (16) arranged in an upper region (I) of the reactor interior (RI) for removing exhaust gas from the container (10). The invention further relates to a method for the submerged production of peat moss, which is carried out using a photobioreactor (1) according to the invention.

The invention provides a spore propagation method of cibotium barometz, and relates to the technical field of plant propagation. The method provided by the invention comprises the following steps: sowing Cibotium barometz spores in a composite culture medium, culturing under the conditions of natural illumination and normal temperature, promoting gametophyte transformation by adopting atomized water spraying instead of traditional oscillation insemination, and culturing seedlings in combination with a non-woven fabric seedling culture container. By means of environmental conditions such as natural light and the like, the bionic technology is adopted for breeding, daily management and protection are simple and economical, low-cost, rough-management and efficient batch seedling raising are achieved, the rate of converting gametophytes into sporophytes is greatly increased, the period from spores to transplantable seedlings of cibotium barometz is shortened to 70-80 days, the transplanting survival rate can be increased to 100%, and the survival rate of cibotium barometz is increased to 50%. And a seedling raising guarantee is provided for batch planting of cibotium barometz.

The invention discloses application of a kelp endogenous promoter in kelp breeding and cultivation and a method, and relates to the technical field of biology. The invention provides a kelp endogenous promoter. The sequence of the kelp endogenous promoter is selected from one of the following sequences: (1) an amino acid sequence as shown in any one of SEQ ID NO: 1-3; (2) a sequence obtained by substituting, deleting or adding one or more nucleotides to the sequence shown in any one of SEQ ID NO: 1 to SEQ ID NO: 3; and (3) a sequence having 80% homology with the sequence as shown in SEQ ID NO: 1-3. The promoter can drive gene specific expression or main expression in a kelp gametophyte, can realize effective expression of a GUS reporter gene in transgenic kelp, and promotes development of kelp genetic engineering and functional genomics research.

This invention discloses a simple and rapid breeding method for artificially synthesizing wheat. It involves inducing incomplete meiosis in *Gnaphalium affine*, a close relative of wheat, under high red light (R / FR≥1.4) to produce unmeiotic divisions. This method falls under the field of crop breeding technology and includes the following steps: *Gnaphalium affine* (DD) cultured in a high red light (R / FR≥1.4) environment undergoes incomplete meiosis during reproductive growth to produce unmeiotic gametes. These gametes are then used as the male parent and crossed with *Langdon* (AABB). The resulting F1 generation (ABDD) is tetraploid. The F2 generation obtained by self-pollination of this F1 generation is hexaploid (AABBDD). Furthermore, the artificially synthesized hexaploid wheat offspring can be stably inherited.

Methane production in ruminants can be reduced by improving rumen fermentation efficiency through dietary supplementation with Asparagopsis taxiformis. This disclosure relates to a method for producing A. taxiformis and its use. In a specific form, this disclosure relates to a method for producing tetrasporophytes and gametophytes of A. taxiformis by environmental manipulation.

The application provides a kind of industrialized breeding method of Undaria pinnatifida seedling, and belongs to the technical field of seedling breeding.The application provides an industrialized breeding method of Undaria pinnatifida seedling, comprising the following steps: respectively propagating female and male gametophytes of Undaria pinnatifida to obtain female and male gametophytes for inoculation; mixing the female and male gametophytes for inoculation, inoculating to the seedling curtain, and carrying out seedling culture.The industrialized breeding method of Undaria pinnatifida seedling of the application is suitable for industrialized breeding of Undaria pinnatifida seedling in high temperature areas, and has the advantages of short cycle, low cost and high quality of seedling.

The present application discloses a plant pollen wall development related protein EXA3, a coding gene thereof and an application. The protein provided by the present application is a protein of (a) or (b) as follows: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO. 1; (b) a protein derived from SEQ ID NO. 1, which is substituted and / or deleted and / or added with one or more amino acid residues and related to plant pollen wall development. The plant pollen development related protein of the present application affects the male gametophyte development process of a plant. Inhibition of the expression of the protein coding gene can cause abnormal development of the male gametophyte of the plant and affect the formation of grains, so that a transgenic plant with abnormal pollen wall development and a transgenic plant with male sterility can be cultivated.

The invention discloses a module for preparing male gametophyte sterility and a preparation method and application thereof, the module is formed by connecting a pollen specific promoter, an interference element of an OsPTDa gene and an interference element of an OsPTDb gene in series through tRNA-Gly, and the gametophyte male sterility module can be obtained after the elements are assembled. The gametophyte male sterility module disclosed by the invention can down-regulate the expression of male gamete development related genes OsPTDa and OsPTDb at the same time, so that the pollen insemination ability is lost, gametophyte male sterility is generated, and the gametophyte male sterility module plays an important role in preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The invention provides a kelp sex specific molecular marker and application thereof, wherein the nucleotide sequence of a male marker fragment is SEQ ID NO: 1, and the nucleotide sequence of a female marker fragment is SEQ ID NO: 2. A gene sequence fragment obtained by screening is from a conserved homologous gene in a sex determining region of brown algae. The screened sex identification marker primer sequence is from a region with the highest similarity in a homologous gene coding sequence, the sex of the kelp gametophyte can be rapidly identified only through a pair of degenerate primers, the efficiency is high, the accuracy is high, and the interspecific universality is high. The method is suitable for identification of male and female mixed samples, distinguishing of parthenogenesis source and sexual reproduction source kelp sporophytes and sex identification of gametophytes of other species of kelp, and important support is provided for efficient management and research and utilization of a kelp gametophyte germplasm bank.

This invention discloses a multi-layer vertical seedling device and method for kelp. The seedling device includes a seedling frame and several layers of seedling trays. The height of the seedling trays is adjustable. A dynamic shading curtain is installed at the top of the seedling frame. A supplemental lighting unit, a flow control unit, and an environmental monitoring unit are installed on each seedling tray. Normal seedling cultivation is performed on the first layer of seedling trays. In the early stage of seedling attachment on the second layer of seedling trays, white light is emitted by the supplemental lighting unit of the second layer to promote the attachment and germination of zoospores to form gametophytes. Subsequently, the supplemental lighting unit of the second layer of seedling trays emits red and blue light, with the proportion of red light being greater than that of blue light. During the extended cultivation stage on the second layer of seedling trays, moderate light intensity red light is used as the main source and blue light as a supplement to slow growth while ensuring the accumulation of photosynthetic products, increasing the chlorophyll content of seedlings, enhancing adhesion, reducing diseases and seedling rot, and improving the stability and yield of seedlings.

The invention discloses a gametophyte male sterility related gene OsPTDb and application thereof. The amino acid sequence of the OsPTDb gene is as shown in SEQ ID NO. 1; the OsPTDb gene encodes an ascorbic acid oxidase which is an essential gene for male gametophyte development, the insemination of the male gametophyte cannot be completed and gametophyte male sterility is generated due to the abnormal function of the gene, and the OsPTDb gene plays an important role in the aspects of preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The invention discloses a first-aid conservation method for a field eradication species Neocheiropteris trilligera, and the method comprises the following steps: resuscitation and culture of nephila specimen spores, induction of green globular bodies (GGBs) by a gametophyte homogenization method, multiplication culture of the green globular bodies, differentiation culture of the green globular bodies, and culture and transplanting of tissue culture seedlings, and further comprises the following steps: resuscitation and culture of nephila specimen spores, induction of the green globular bodies (GGBs) by a gametophyte homogenization method, propagation culture of the green globular bodies (GGBs), differentiation culture of the green globular bodies (GGBs), propagation culture of the tissue culture seedlings (GGBs) and transplantation of the tissue culture seedlings (GGBs). By means of the method, a large number of cynanchum otophyllum plants can be obtained under the condition that no survival cynanchum otophyllum plants exist, rescue conservation of field eradication cynanchum otophyllum is achieved, reference is provided for rescue conservation of other field eradication pteridophyte, and the method has important significance on diversity protection of Chinese pteridophyte.

The present invention relates to the field of expression of recombinant glycoproteins in plants. In particular, the present invention relates to a method of producing a genetically modified plant capable of producing glycoproteins harbouring paucimannosidic N-glycans, to a method of producing a glycoprotein of interest harbouring paucimannosidic N-glycans and to genetically modified plants, plant cells, protoplasts, protonemas, gametophytes, sporophytes or spores produced with or used in such methods.

The invention discloses a chimeric interference pollen lethal module as well as a preparation method and application thereof. The module consists of a pollen specific promoter, a chimeric interference element of an OsPTDa gene and an OsPTDb gene, and a plant endogenous terminator. After the elements are assembled, pollen can be killed. The pollen lethal module disclosed by the invention can accurately down-regulate the expression of male gametophyte development related genes OsPTDa and OsPTDb in the same time and space, so that pollen germination is abnormal, transgenic pollen cannot generate offspring, gametophyte male sterility is generated, and the pollen lethal module has important value in the aspects of preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The invention discloses a plant pollen wall development related protein OsEXA5 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.1, is related to plant pollen wall development and is derived from SEQ ID NO.1. The plant pollen development related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene can lead to abnormal development of plant male gametophytes and influence the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterile transgenic plants can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

The invention discloses plant male sterility related aldehyde oxidase EXA1 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO.1, is related to plant aldehyde oxidase and is derived from SEQ ID NO.1. The plant aldehyde oxidase related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene leads to abnormal development of plant male gametophytes and influences the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterility can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention relates to the field of bioengineering technology, and proposes a culture medium and method for cultivating gametophytes of *Bryophytum pumilus*. The spore culture medium for *Bryophytum pumilus* gametophytes comprises the following components: MgSO4·7H2O 250 mg / L, KH2PO4 250 mg / L, FeSO4·4H2O 12.5 mg / L, KNO3 1010 mg / L, ammonium tartrate 921 mg / L, CaCl2·2H2O 147 mg / L, and glucose 5000 mg / L. Through the above technical solution, this invention can achieve the formation and propagation of *Bryophytum pumilus* gametophytes in a short time, providing a feasible approach for the cultivation and subculturing of *Bryophytum pumilus*, and providing seed material and propagation technology for using mosses in environmental remediation and landscaping.

The invention relates to germplasm preservation, propagation and seedling cultivation of undaria pinnatifida, in particular to a method for large-scale updating and rapid propagation of undaria pinnatifida gametophyte germplasm. Specifically, obtained mature homozygous double (DH) sporophytes are dried in shade and stimulated to diffuse a large number of zoospores, and the spores germinate to form gametophytes consistent with parent genotypes, so that large-scale updating and rapid propagation of gametophyte germplasm are realized. The method disclosed by the invention effectively solves the technical bottleneck that a large number of gametophyte clones are difficult to obtain in a short time due to slow cell division of gametophytes from single cells, and has important significance on creation of new undaria pinnatifida hybrid varieties and large-scale seed production.

The invention discloses a method for efficiently obtaining porphyra yezoensis fructospore seedlings, and relates to the field of sexual reproduction and breeding of porphyra. The method comprises the following steps: crushing laver by using a wall breaking machine, crushing thalli into fragments, collecting the fragments, culturing the fragments for several days, and adding shells after fruit spores are produced. The shells and the fragments are mixed, ventilated or subjected to static culture, and a large number of fruit spore seedlings can be obtained. According to the mechanical crushing method, carpospores can be intensively and massively diffused within a short time, so that a large amount of carpospores drill into shells and rapidly develop into shell protonema, and the efficiency of obtaining porphyra yezoensis carpospore seedlings can be improved.

The invention belongs to the technical field of artificial breeding of plants, and discloses an artificial rapid breeding and efficient cultivation method of cibotium barometz, which comprises the following steps: spore disinfection and germination: collecting mature spores of cibotium barometz, performing surface disinfection, inoculating into a first culture medium, and performing germination culture to obtain gametophytes; seedling induction and rooting: transferring the gametophyte into a second culture medium, inducing the gametophyte to develop into a seedling and root, and culturing until the seedling has a robust root system; acclimatization and transplanting: transplanting the rooted seedlings into an acclimatization matrix, performing acclimatization culture in an acclimatization environment with the humidity controlled to be 88-92% and the temperature controlled to be 18-22 DEG C, and then transplanting the seedlings into natural soil. According to the method, the pollution rate is reduced from 50% to 15%, and the disinfection success rate of the cibotium barometz spores is greatly improved; growth hormone is added, so that the germination period of spores and the rooting period of seedlings are shortened; the survival rate of the seedlings can reach 80%, and the success rate of seedling hardening is increased.