56 results about "Gametophyte" patented technology

The invention discloses a rapid spore propagation method of a cyatheridae rare fern type pteridium aquilinum var. Spinulosa var. Spinulosa. Comprising the following steps: (1) collecting and storing spores; (2) selecting and preparing a spore culture medium; (3) spore treatment and sowing; (4) culturing spores; (5) observing and maintaining gametophytes; (6) transplanting sporophyte young plants; (7) seedling management and protection; (8) transplanting seedlings. When seedlings in the seedling raising box grow to about 5-8 cm high, the seedling raising box is moved into a non-imitation cloth seedling raising bag in an outdoor greenhouse nursery. Compared with tissue culture, the method has the advantages of high success rate, simplicity and high efficiency. A manual climatic box is not needed in a spore-gametophyte cultivation stage, but on a light-cared cultivation frame, so that the operation is simple and easy, and the limitation of space is avoided.

The invention discloses a moss ecological brick and a preparation method thereof, and relates to the technical field of ecological restoration, the moss ecological brick comprises a moss gametophyte matrix and a brick body, the brick body comprises the following raw materials: a tailing composite material, metakaolin, an acid activator and water, and the tailing composite material comprises phosphate tailings and iron tailings. The phosphate tailings and the iron tailings can provide necessary phosphorus elements, other trace elements and the like for the growth of the moss, and the acid activator in the brick body not only can provide a weak acid environment to facilitate the good growth of the moss, but also can solve the problem of saltpetering of the brick body in the environment; the growth of the moss can effectively relieve sulfate erosion of the acid-activated material, and a good curing environment is provided for the acid-activated concrete brick body. Therefore, in the moss ecological brick provided by the invention, the acid-activated concrete brick body can supplement and promote the growth of moss.

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

The invention relates to genetic breeding, in particular to a method for cultivating kelp triploid by inducing a monosexual dihaploid (DH) gametophyte. The method specifically comprises the following steps: establishing a kelp DH sporophyte culture system, inducing DH sporophytes to generate unisexual DH gametophytes (2n) through spore-free propagation, and then hybridizing the unisexual DH gametophytes (2n) with haploid (n) gametophytes with opposite sex to obtain kelp triploid (3n) sporophytes. And determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometry, thereby identifying the chromosome ploidy of the hybrid sporophyte as 3n. The method can be used for cultivating new varieties of high-yield, high-quality, stress-resistant and sterile kelp, and has important significance for developing genetic breeding and new variety creation research in kelp and even kelp brown algae.

The present invention provides a moss gametophyte coating agent, comprising: 80-140 parts by weight of a composite additive, 40-60 parts of a film-forming agent, 10-20 parts of a medicament, 8-20 parts of an antifreeze agent, 200-300 parts of a nutrient matrix, 0.01-1 part of a growth regulator, 20-50 parts of an emulsifier, 10-30 parts of a dispersant, 10-30 parts of an anti-freezing agent, 10-20 parts of an anti-foaming agent, and 600-800 parts of deionized water; wherein, the composite additive is modified montmorillonite, the medicament is a combination of chitosan and garlic extract, and the antifreeze agent is an algal extract. The moss gametophyte coating agent provided by the present invention is used for coating the moss gametophyte, significantly improving the survival rate, germination rate of the moss gametophyte and the stress tolerance to habitats such as high cold, drought and barrenness, and effectively breaking through the technical bottleneck of the field breeding of the moss gametophyte.

Herein is described a method devised to cryopreserve mosquito eggs, viable upon subsequent thawing. The method reproducibly yields >25% hatched first instar larvae of Anopheles-species mosquitoes from cryopreserved eggs. The majority of hatched larvae continue to develop normally (87% pupation), through to adults (98%, 50% of which are female) which subsequently blood feed and produce viable second generation embryos that also develop normally. Adult mosquitoes obtained from cryopreserved embryos are able to be infected with Plasmodium falciparum gametocytes and produce salivary gland sporozoites in similar numbers to control mosquitoes.

The present invention relates to a Babesia antigen comprising at least a portion of a gametocyte HAPLESS2 / GCS1 (HAP2) protein, vectors and cells expressing such antigen, compositions and kits comprising such antigens, and methods of using such antigens to interfere with the Babesia transmission by competent ticks.

The invention discloses a kelp gametophyte gene editing method and application, and belongs to the technical field of gene editing, the kelp gametophyte gene editing method comprises the following steps: preparing a kelp gametophyte receptor; assembling an RNP complex and preparing a microcarrier; introducing the RNP compound into a kelp gametophyte by using a gene gun; and recovering culture and screening. According to the technical scheme, the dependency of a microinjection technology on precise instruments and professional operation skills is eliminated, efficient gene delivery can be achieved through a standardized gene gun operation process, the technical threshold is greatly reduced, and the kelp gametophyte gene editing method has remarkable technical advantages. According to the method, the high-throughput characteristic of a gene gun technology is fully exerted, multiple mutants can be obtained through one-time bombardment, the experiment efficiency is greatly improved, and powerful support is provided for kelp large-scale gene function research and molecular breeding.

This invention provides for the development of singularity-inducing substances and their use in generating singularity plants and in doubling singularity technology. [Solution] The present invention provides a plant comprising a polynucleic acid encoding a mutated undetermined gametophyte (ig) protein and a polynucleic acid encoding a mutated centromere or kinetochore protein. The mutated centromere or kinetochore protein is preferably CENH3. The mutated ig and the centromere or kinetochore protein together result in singular induction activity, for example, paternal singular induction activity. The present invention further provides a method for producing such a plant and its use.

The invention provides an induction culture method for embryonic calluses of pinus khasys, and relates to the technical field of biologication.The method comprises the steps that male flowers of the pinus khasys are collected, and the collected male flowers are subjected to surface disinfection treatment; soaking the male flowers of the pinus khasys subjected to surface disinfection in a detoxification solution, and performing detoxification treatment; cleaning the detoxified male flower explant of pinus khasys, and absorbing moisture; and taking out the explant of the pinus khasys after moisture absorption, separating out a male gametophyte of the explant, and placing the male gametophyte in a culture medium for dark culture until callus is induced. According to the method, the male gametophyte of the pinus khasys is adopted for explant cultivation of the pinus khasys, anther serves as a reproductive organ of the plant, genetic information is stable, and the good characters of the pinus khasys can be kept; by selecting the pinus kesiya anther as a breeding material, stable separation of offspring can be realized as early as possible, and the reliability and accuracy of selection are improved; in addition, the calluses induced by the pollen can maintain the excellent characters of the female parent, and have important significance on subsequent superior plant propagation.

The invention relates to mir-331-3p as a biomarker for predicting malaria transmission and application of the mir-331-3p, and belongs to the technical field of biology. The invention aims to provide a more accurate biomarker, and solves the technical defect that the existing index cannot effectively evaluate the malaria spreading risk. The invention provides a host-derived miR-331-3p (miR-331-3p) used as a malaria transmission prediction marker. The detection kit containing the marker is constructed by detecting the expression level of miR-331-3p in host serum and combining the characteristic that the miR-331-3p regulates and controls the development of plasmodium in mosquito bodies in a cross-species manner. Compared with traditional gametophyte detection, the method has the advantages that the sensitivity is improved, and the technical bottleneck that the gametophyte rate and the transmission capacity are unhooked is broken through; 2) non-invasive detection is realized, and only trace serum samples are needed; and 3) establishing a host-medium bidirectional regulation and control model, and providing a new target spot for malaria transmission blocking.

A switch to haploid embryogenesis is controlled by the activity of histone deacetylases (HDACs). Blocking HDAC activity with HDAC inhibitors (HDACi), e.g., trichostatin A (TSA), in Brassica napus, B. rapa, Arabidopsis thaliana, and Capsicum annuum male gametophytes leads to a large increase in the proportion of cells that undergo embryogenic growth. In B. napus, treatment with one specific HDACi (SAHA) improves the conversion (i.e., germination) of these embryos into seedlings. Existing methods of culturing microspores of angiosperm plants following stress to produce haploid embryos, haploid plants, and double haploid plants can be improved by adding HDACi to the culture medium. Advantageously, species hitherto recalcitrant to haploid embryogenesis via microspore culture are rendered useful when using HDACi. Haploid and double haploid plants are of industrial application in the plant breeding programmes.

The invention relates to a photobioreactor (1) for the submerged production of sphagnum moss, comprising a container (10) with a reactor interior (RI) comprising at least a portion of the reactor interior (RI). The container (10) has a first compartment (11) and a second compartment (12) arranged above the first compartment (11), wherein the compartments (11, 12, 13) form at least a portion of the reactor interior (RI), are substantially transparent, and have a height (H) to diameter (D) ratio of each compartment of 1 to 2.6. Furthermore, the container (10) has a separating element (14) arranged between the first and the second compartment (11, 12) having a plurality of openings (141) for fluid-conducting connection between the compartments (11, 12, 13) and a closable access opening for adding and removing mineral nutrient medium and peat moss gametophytes into or from at least part of the reactor interior (RI).Furthermore, the container (10) comprises a gas distributor (15) arranged centrally in a lower region (II) of the reactor interior (RI), with a plurality of gas openings for supplying gas into at least a part of the reactor interior (RI) along a gassed sub-region of a photobioreactor cross-sectional area, and a gas outlet (16) arranged in an upper region (I) of the reactor interior (RI) for removing exhaust gas from the container (10). The invention further relates to a method for the submerged production of peat moss, which is carried out using a photobioreactor (1) according to the invention.

The invention provides a spore propagation method of cibotium barometz, and relates to the technical field of plant propagation. The method provided by the invention comprises the following steps: sowing Cibotium barometz spores in a composite culture medium, culturing under the conditions of natural illumination and normal temperature, promoting gametophyte transformation by adopting atomized water spraying instead of traditional oscillation insemination, and culturing seedlings in combination with a non-woven fabric seedling culture container. By means of environmental conditions such as natural light and the like, the bionic technology is adopted for breeding, daily management and protection are simple and economical, low-cost, rough-management and efficient batch seedling raising are achieved, the rate of converting gametophytes into sporophytes is greatly increased, the period from spores to transplantable seedlings of cibotium barometz is shortened to 70-80 days, the transplanting survival rate can be increased to 100%, and the survival rate of cibotium barometz is increased to 50%. And a seedling raising guarantee is provided for batch planting of cibotium barometz.

The invention discloses application of a kelp endogenous promoter in kelp breeding and cultivation and a method, and relates to the technical field of biology. The invention provides a kelp endogenous promoter. The sequence of the kelp endogenous promoter is selected from one of the following sequences: (1) an amino acid sequence as shown in any one of SEQ ID NO: 1-3; (2) a sequence obtained by substituting, deleting or adding one or more nucleotides to the sequence shown in any one of SEQ ID NO: 1 to SEQ ID NO: 3; and (3) a sequence having 80% homology with the sequence as shown in SEQ ID NO: 1-3. The promoter can drive gene specific expression or main expression in a kelp gametophyte, can realize effective expression of a GUS reporter gene in transgenic kelp, and promotes development of kelp genetic engineering and functional genomics research.

This invention discloses a simple and rapid breeding method for artificially synthesizing wheat. It involves inducing incomplete meiosis in *Gnaphalium affine*, a close relative of wheat, under high red light (R / FR≥1.4) to produce unmeiotic divisions. This method falls under the field of crop breeding technology and includes the following steps: *Gnaphalium affine* (DD) cultured in a high red light (R / FR≥1.4) environment undergoes incomplete meiosis during reproductive growth to produce unmeiotic gametes. These gametes are then used as the male parent and crossed with *Langdon* (AABB). The resulting F1 generation (ABDD) is tetraploid. The F2 generation obtained by self-pollination of this F1 generation is hexaploid (AABBDD). Furthermore, the artificially synthesized hexaploid wheat offspring can be stably inherited.

Methane production in ruminants can be reduced by improving rumen fermentation efficiency through dietary supplementation with Asparagopsis taxiformis. This disclosure relates to a method for producing A. taxiformis and its use. In a specific form, this disclosure relates to a method for producing tetrasporophytes and gametophytes of A. taxiformis by environmental manipulation.

The application provides a kind of industrialized breeding method of Undaria pinnatifida seedling, and belongs to the technical field of seedling breeding.The application provides an industrialized breeding method of Undaria pinnatifida seedling, comprising the following steps: respectively propagating female and male gametophytes of Undaria pinnatifida to obtain female and male gametophytes for inoculation; mixing the female and male gametophytes for inoculation, inoculating to the seedling curtain, and carrying out seedling culture.The industrialized breeding method of Undaria pinnatifida seedling of the application is suitable for industrialized breeding of Undaria pinnatifida seedling in high temperature areas, and has the advantages of short cycle, low cost and high quality of seedling.

The invention relates to genetic breeding, in particular to a method for cultivating undaria pinnatifida triploid sporophytes by inducing homozygous diploid gametophytes. The method comprises the following steps: inducing homozygous 2n undaria pinnatifida sporophores to produce homozygous 2n gametophytes through spore-free propagation, and hybridizing the homozygous 2n gametophytes with common haploid (n) gametophytes to obtain undaria pinnatifida triploid (3n) sporophores; and determining the relative content of DNA in the nucleus of the hybrid sporophyte by using a flow cytometer, thereby identifying the ploidy of the chromosome as 3n. The 3n sporophytes are transplanted to the sea for cultivation, and the 3n sporophytes are identified to be incapable of releasing zoospores in the undaria pinnatifida seedling raising (breeding) season, namely infertile. The method can be used for cultivating a new variety of high-yield and high-quality undaria pinnatifida, and has important significance in developing genetic breeding and new variety creation research in undaria pinnatifida and even kelp brown algae.

The present application discloses a plant pollen wall development related protein EXA3, a coding gene thereof and an application. The protein provided by the present application is a protein of (a) or (b) as follows: (a) a protein consisting of an amino acid sequence shown in SEQ ID NO. 1; (b) a protein derived from SEQ ID NO. 1, which is substituted and / or deleted and / or added with one or more amino acid residues and related to plant pollen wall development. The plant pollen development related protein of the present application affects the male gametophyte development process of a plant. Inhibition of the expression of the protein coding gene can cause abnormal development of the male gametophyte of the plant and affect the formation of grains, so that a transgenic plant with abnormal pollen wall development and a transgenic plant with male sterility can be cultivated.

The invention discloses a module for preparing male gametophyte sterility and a preparation method and application thereof, the module is formed by connecting a pollen specific promoter, an interference element of an OsPTDa gene and an interference element of an OsPTDb gene in series through tRNA-Gly, and the gametophyte male sterility module can be obtained after the elements are assembled. The gametophyte male sterility module disclosed by the invention can down-regulate the expression of male gamete development related genes OsPTDa and OsPTDb at the same time, so that the pollen insemination ability is lost, gametophyte male sterility is generated, and the gametophyte male sterility module plays an important role in preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The invention provides a kelp sex specific molecular marker and application thereof, wherein the nucleotide sequence of a male marker fragment is SEQ ID NO: 1, and the nucleotide sequence of a female marker fragment is SEQ ID NO: 2. A gene sequence fragment obtained by screening is from a conserved homologous gene in a sex determining region of brown algae. The screened sex identification marker primer sequence is from a region with the highest similarity in a homologous gene coding sequence, the sex of the kelp gametophyte can be rapidly identified only through a pair of degenerate primers, the efficiency is high, the accuracy is high, and the interspecific universality is high. The method is suitable for identification of male and female mixed samples, distinguishing of parthenogenesis source and sexual reproduction source kelp sporophytes and sex identification of gametophytes of other species of kelp, and important support is provided for efficient management and research and utilization of a kelp gametophyte germplasm bank.

The application is directed to in vitro-reared Plasmodium sporozoites of human host range wherein sporogony from gametocyte stage to sporozoite stage is external to mosquitoes, and methods of producing the same. Provided herein are in vitro-reared infectious Plasmodium sporozoites (SPZ) of human host range, particularly P. falciparum, P. vivax, P. ovale, P. malariae, and P. knowlesi, wherein sporogony from gametocyte stage to sporozoite stage is external to mosquitoes, and methods of producing the same.

This invention discloses a multi-layer vertical seedling device and method for kelp. The seedling device includes a seedling frame and several layers of seedling trays. The height of the seedling trays is adjustable. A dynamic shading curtain is installed at the top of the seedling frame. A supplemental lighting unit, a flow control unit, and an environmental monitoring unit are installed on each seedling tray. Normal seedling cultivation is performed on the first layer of seedling trays. In the early stage of seedling attachment on the second layer of seedling trays, white light is emitted by the supplemental lighting unit of the second layer to promote the attachment and germination of zoospores to form gametophytes. Subsequently, the supplemental lighting unit of the second layer of seedling trays emits red and blue light, with the proportion of red light being greater than that of blue light. During the extended cultivation stage on the second layer of seedling trays, moderate light intensity red light is used as the main source and blue light as a supplement to slow growth while ensuring the accumulation of photosynthetic products, increasing the chlorophyll content of seedlings, enhancing adhesion, reducing diseases and seedling rot, and improving the stability and yield of seedlings.

The invention discloses a method for high-density proliferation of undaria pinnatifida gametophytes, which comprises the following steps: S1, crushing a part of fresh undaria pinnatifida gametophytes into algae segments, and transferring the algae segments into a culture bottle filled with a part of culture medium, S2, placing the culture bottle into an illumination incubator, and carrying out high-density proliferation on the algae segments in the illumination incubator, so as to obtain the high-density proliferation of the undaria pinnatifida gametophytes. And S3, filtering the culture body by using a filter screen, picking out the gametophyte by using tweezers, absorbing excessive moisture by using sterile absorbent paper, weighing the mass of the gametophyte, and putting the gametophyte into an illumination incubator for illumination, so that the gametophyte is obtained. The nitrogen concentration and the phosphorus concentration in the illumination incubator are 11-150 mg / L and 1-10 mg / L respectively. Compared with the prior art, the method for high-density proliferation of the undaria pinnatifida gametophyte has the advantages that the biomass of the undaria pinnatifida gametophyte is increased to be 1.3 times or more of that of the undaria pinnatifida gametophyte when the culture condition is not optimized, large-scale rapid seedling raising of the undaria pinnatifida is facilitated, and the production efficiency is improved.

The invention discloses a gametophyte male sterility related gene OsPTDb and application thereof. The amino acid sequence of the OsPTDb gene is as shown in SEQ ID NO. 1; the OsPTDb gene encodes an ascorbic acid oxidase which is an essential gene for male gametophyte development, the insemination of the male gametophyte cannot be completed and gametophyte male sterility is generated due to the abnormal function of the gene, and the OsPTDb gene plays an important role in the aspects of preventing transgene from escaping along with pollen, breeding sporophyte male nuclear sterility and the like.

The invention discloses a first-aid conservation method for a field eradication species Neocheiropteris trilligera, and the method comprises the following steps: resuscitation and culture of nephila specimen spores, induction of green globular bodies (GGBs) by a gametophyte homogenization method, multiplication culture of the green globular bodies, differentiation culture of the green globular bodies, and culture and transplanting of tissue culture seedlings, and further comprises the following steps: resuscitation and culture of nephila specimen spores, induction of the green globular bodies (GGBs) by a gametophyte homogenization method, propagation culture of the green globular bodies (GGBs), differentiation culture of the green globular bodies (GGBs), propagation culture of the tissue culture seedlings (GGBs) and transplantation of the tissue culture seedlings (GGBs). By means of the method, a large number of cynanchum otophyllum plants can be obtained under the condition that no survival cynanchum otophyllum plants exist, rescue conservation of field eradication cynanchum otophyllum is achieved, reference is provided for rescue conservation of other field eradication pteridophyte, and the method has important significance on diversity protection of Chinese pteridophyte.

The present invention relates to the field of expression of recombinant glycoproteins in plants. In particular, the present invention relates to a method of producing a genetically modified plant capable of producing glycoproteins harbouring paucimannosidic N-glycans, to a method of producing a glycoprotein of interest harbouring paucimannosidic N-glycans and to genetically modified plants, plant cells, protoplasts, protonemas, gametophytes, sporophytes or spores produced with or used in such methods.

The invention provides a method for constructing a kelp core germplasm and identifying the germplasm based on an SNP (Single Nucleotide Polymorphism) molecular marker, which comprises the following steps: collecting kelp gametophyte germplasm with complete and accurate source information, identifying and screening the SNP marker, and determining an optimal core germplasm group by utilizing an EN-MR / CE model and comparing genetic diversity; 130 SNP marker sets which have polymorphism and are relatively uniformly distributed on chromosomes are further screened, so that 91 core germplasm can be effectively distinguished. The method can reflect genetic diversity of germplasm resources in a kelp germplasm bank, efficiently select core germplasm, accurately identify germplasm materials, and provide technical support for improving kelp germplasm preservation and utilization efficiency.