20 results about "Gametophyte" patented technology

The invention discloses a method for cultivating triploid kelp sporophytes and identifying ploidy of induced protonema, and belongs to the field of kelp breeding. The method is based on a capillary glass needle separation technology for inducing filamentous somatic cells by kelp and a molecular biological technology for specific marking of sex of kelp gametophytes. The sex specific marker of the kelp gametophyte is used for carrying out PCR (Polymerase Chain Reaction) amplification and 1.5% agarose gel electrophoresis detection on the separated induction protonema single-cell cloning line, so that the genetic sex of the kelp induction protonema can be accurately identified; and the ploidy of the induced protonema of a part of varieties (lines) is directly determined from the molecular level. The method for synchronously detecting the genetic sex and ploidy of the induced protonema breaks through the bottlenecks of chromosome counting and flow cytometry operation, can be completed only through one-time PCR amplification, and is simple, convenient, rapid, short in time consumption, capable of completing detection of a plurality of samples at the same time and low in cost.

The present invention relates to a Babesia antigen comprising at least a portion of a gametocyte HAPLESS2 / GCS1 (HAP2) protein, vectors and cells expressing such antigen, compositions and kits comprising such antigens, and methods of using such antigens to interfere with the Babesia transmission by competent ticks.

The invention relates to a photobioreactor (1) for the submerged production of sphagnum moss, comprising a container (10) with a reactor interior (RI) comprising at least a portion of the reactor interior (RI). The container (10) has a first compartment (11) and a second compartment (12) arranged above the first compartment (11), wherein the compartments (11, 12, 13) form at least a portion of the reactor interior (RI), are substantially transparent, and have a height (H) to diameter (D) ratio of each compartment of 1 to 2.6. Furthermore, the container (10) has a separating element (14) arranged between the first and the second compartment (11, 12) having a plurality of openings (141) for fluid-conducting connection between the compartments (11, 12, 13) and a closable access opening for adding and removing mineral nutrient medium and peat moss gametophytes into or from at least part of the reactor interior (RI).Furthermore, the container (10) comprises a gas distributor (15) arranged centrally in a lower region (II) of the reactor interior (RI), with a plurality of gas openings for supplying gas into at least a part of the reactor interior (RI) along a gassed sub-region of a photobioreactor cross-sectional area, and a gas outlet (16) arranged in an upper region (I) of the reactor interior (RI) for removing exhaust gas from the container (10). The invention further relates to a method for the submerged production of peat moss, which is carried out using a photobioreactor (1) according to the invention.

This invention discloses a simple and rapid breeding method for artificially synthesizing wheat. It involves inducing incomplete meiosis in *Gnaphalium affine*, a close relative of wheat, under high red light (R / FR≥1.4) to produce unmeiotic divisions. This method falls under the field of crop breeding technology and includes the following steps: *Gnaphalium affine* (DD) cultured in a high red light (R / FR≥1.4) environment undergoes incomplete meiosis during reproductive growth to produce unmeiotic gametes. These gametes are then used as the male parent and crossed with *Langdon* (AABB). The resulting F1 generation (ABDD) is tetraploid. The F2 generation obtained by self-pollination of this F1 generation is hexaploid (AABBDD). Furthermore, the artificially synthesized hexaploid wheat offspring can be stably inherited.

Methane production in ruminants can be reduced by improving rumen fermentation efficiency through dietary supplementation with Asparagopsis taxiformis. This disclosure relates to a method for producing A. taxiformis and its use. In a specific form, this disclosure relates to a method for producing tetrasporophytes and gametophytes of A. taxiformis by environmental manipulation.

The application provides a kind of industrialized breeding method of Undaria pinnatifida seedling, and belongs to the technical field of seedling breeding.The application provides an industrialized breeding method of Undaria pinnatifida seedling, comprising the following steps: respectively propagating female and male gametophytes of Undaria pinnatifida to obtain female and male gametophytes for inoculation; mixing the female and male gametophytes for inoculation, inoculating to the seedling curtain, and carrying out seedling culture.The industrialized breeding method of Undaria pinnatifida seedling of the application is suitable for industrialized breeding of Undaria pinnatifida seedling in high temperature areas, and has the advantages of short cycle, low cost and high quality of seedling.

This invention discloses a multi-layer vertical seedling device and method for kelp. The seedling device includes a seedling frame and several layers of seedling trays. The height of the seedling trays is adjustable. A dynamic shading curtain is installed at the top of the seedling frame. A supplemental lighting unit, a flow control unit, and an environmental monitoring unit are installed on each seedling tray. Normal seedling cultivation is performed on the first layer of seedling trays. In the early stage of seedling attachment on the second layer of seedling trays, white light is emitted by the supplemental lighting unit of the second layer to promote the attachment and germination of zoospores to form gametophytes. Subsequently, the supplemental lighting unit of the second layer of seedling trays emits red and blue light, with the proportion of red light being greater than that of blue light. During the extended cultivation stage on the second layer of seedling trays, moderate light intensity red light is used as the main source and blue light as a supplement to slow growth while ensuring the accumulation of photosynthetic products, increasing the chlorophyll content of seedlings, enhancing adhesion, reducing diseases and seedling rot, and improving the stability and yield of seedlings.

The invention discloses a plant pollen wall development related protein OsEXA5 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.1, is related to plant pollen wall development and is derived from SEQ ID NO.1. The plant pollen development related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene can lead to abnormal development of plant male gametophytes and influence the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterile transgenic plants can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

Methane production in ruminant animals may be reduced by improving rumen fermentation efficiency through dietary supplementation of Asparagopsis taxiformis. The present disclosure relates to a method of producing A. taxiformis and uses thereof. In particular forms, the present disclosure relates to methods of producing a tetrasporophyte and a gametophyte of A. taxiformis through environmental manipulations.

The invention discloses plant male sterility related aldehyde oxidase EXA1 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO.1, is related to plant aldehyde oxidase and is derived from SEQ ID NO.1. The plant aldehyde oxidase related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene leads to abnormal development of plant male gametophytes and influences the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterility can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention relates to the field of bioengineering technology, and proposes a culture medium and method for cultivating gametophytes of *Bryophytum pumilus*. The spore culture medium for *Bryophytum pumilus* gametophytes comprises the following components: MgSO4·7H2O 250 mg / L, KH2PO4 250 mg / L, FeSO4·4H2O 12.5 mg / L, KNO3 1010 mg / L, ammonium tartrate 921 mg / L, CaCl2·2H2O 147 mg / L, and glucose 5000 mg / L. Through the above technical solution, this invention can achieve the formation and propagation of *Bryophytum pumilus* gametophytes in a short time, providing a feasible approach for the cultivation and subculturing of *Bryophytum pumilus*, and providing seed material and propagation technology for using mosses in environmental remediation and landscaping.

The invention discloses a method for efficiently obtaining porphyra yezoensis fructospore seedlings, and relates to the field of sexual reproduction and breeding of porphyra. The method comprises the following steps: crushing laver by using a wall breaking machine, crushing thalli into fragments, collecting the fragments, culturing the fragments for several days, and adding shells after fruit spores are produced. The shells and the fragments are mixed, ventilated or subjected to static culture, and a large number of fruit spore seedlings can be obtained. According to the mechanical crushing method, carpospores can be intensively and massively diffused within a short time, so that a large amount of carpospores drill into shells and rapidly develop into shell protonema, and the efficiency of obtaining porphyra yezoensis carpospore seedlings can be improved.

The invention belongs to the technical field of artificial breeding of plants, and discloses an artificial rapid breeding and efficient cultivation method of cibotium barometz, which comprises the following steps: spore disinfection and germination: collecting mature spores of cibotium barometz, performing surface disinfection, inoculating into a first culture medium, and performing germination culture to obtain gametophytes; seedling induction and rooting: transferring the gametophyte into a second culture medium, inducing the gametophyte to develop into a seedling and root, and culturing until the seedling has a robust root system; acclimatization and transplanting: transplanting the rooted seedlings into an acclimatization matrix, performing acclimatization culture in an acclimatization environment with the humidity controlled to be 88-92% and the temperature controlled to be 18-22 DEG C, and then transplanting the seedlings into natural soil. According to the method, the pollution rate is reduced from 50% to 15%, and the disinfection success rate of the cibotium barometz spores is greatly improved; growth hormone is added, so that the germination period of spores and the rooting period of seedlings are shortened; the survival rate of the seedlings can reach 80%, and the success rate of seedling hardening is increased.

This invention provides a sex-specific molecular marker for kelp and its application, wherein the nucleotide sequence of the male marker fragment is SEQ ID NO:1, and the nucleotide sequence of the female marker fragment is SEQ ID NO:2. The gene sequence fragments obtained by this invention are derived from conserved homologous genes in the sex-determining regions of brown algae. The primer sequences for the selected sex identification markers are derived from the regions with the highest similarity in the coding sequences of the homologous genes. This method enables rapid identification of the sex of kelp gametophytes using only a pair of degenerate primers, offering high efficiency, high accuracy, and high interspecies compatibility. This method is applicable to the identification of mixed male and female samples, the differentiation of kelp sporophytes from parthenogenesis and sexual reproduction, and the sex identification of gametophytes in other species of the Laminariales order, providing important support for the efficient management and research utilization of kelp gametophyte germplasm banks.

This invention provides a method for inducing and culturing embryogenic callus from *Pinus kesiya*, relating to the field of biotechnology. The method includes: collecting male cones of *Pinus kesiya* and performing surface disinfection on the collected male cones; immersing the surface-disinfected male cones in a detoxification solution for detoxification; washing the detoxified male flower explants and drying them; removing the dried explants, separating the male gametophytes from the explants, and culturing them in the dark in a culture medium until callus is induced. This method uses male gametophytes of *Pinus kesiya* for explant cultivation. Anthers, as reproductive organs of plants, possess stable genetic information, which helps maintain the superior traits of *Pinus kesiya*. Using *Pinus kesiya* anthers as breeding material allows for early and stable segregation of offspring, improving the reliability and accuracy of selection. Furthermore, the callus induced by pollen can maintain the superior traits of the maternal parent, which is of great significance for subsequent propagation of superior plants.

The invention provides a method for constructing a kelp core germplasm and identifying the germplasm based on an SNP (Single Nucleotide Polymorphism) molecular marker. According to the method, kelp gametophyte germplasm with complete and accurate source information is collected, SNP site detection and screening are performed, and an optimal core germplasm group is determined by utilizing an EN-MR / CE model and combining genetic diversity comparison; 130 SNP markers which are uniformly distributed on chromosomes and rich in polymorphism are obtained through further screening, and accurate identification of 91 core germplasm can be realized. Meanwhile, the core SNP marker can be used for effectively distinguishing germplasm materials from different kelp cultivated strains. According to the method, the genetic diversity background of the kelp germplasm bank can be truly reflected, the core germplasm is efficiently screened, the germplasm material is identified, and technical support is provided for improving the storage and utilization efficiency of the kelp germplasm.

The invention discloses a plant pollen wall development related protein as well as a coding gene and application thereof. The protein provided by the invention is a protein composed of an amino acid sequence as shown in SEQ ID NO. 1. The plant pollen development related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene can lead to abnormal development of plant male gametophytes and influence the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterile transgenic plants can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention relates to the technical field of algae moss gametophyte germplasm resource preservation, in particular to an algae moss gametophyte germplasm resource long-term ultralow-temperature cryopreservation method which comprises the steps of algae moss gametophyte sterile culture (selection of light green gametophyte subculture, 14 / 10 DEG C day and night temperature change and 12 / 12-h light and dark period culture), preparation of Benecke Takakia liquid / solid culture medium, pre-freezing culture medium and other protective agents. The long-term ultralow-temperature cryopreservation method for the algae moss gametophyte germplasm resources comprises the following steps: performing cold culture on algae moss at 4 DEG C, cutting stem tips for pre-culture, treating the stem tips, embedding to form embedded beads, loading a protective agent in a gradient manner, storing for 1 day at-20 DEG C, performing long-term cryopreservation at-80 DEG C, unfreezing and washing at 42 DEG C during resuscitation, and inoculating a solid culture medium. The algae moss germplasm can be effectively protected, and the environment and gene diversity are guaranteed.