9 results about "Gametophyte" patented technology

The present invention relates to a Babesia antigen comprising at least a portion of a gametocyte HAPLESS2 / GCS1 (HAP2) protein, vectors and cells expressing such antigen, compositions and kits comprising such antigens, and methods of using such antigens to interfere with the Babesia transmission by competent ticks.

The invention relates to a photobioreactor (1) for the submerged production of sphagnum moss, comprising a container (10) with a reactor interior (RI) comprising at least a portion of the reactor interior (RI). The container (10) has a first compartment (11) and a second compartment (12) arranged above the first compartment (11), wherein the compartments (11, 12, 13) form at least a portion of the reactor interior (RI), are substantially transparent, and have a height (H) to diameter (D) ratio of each compartment of 1 to 2.6. Furthermore, the container (10) has a separating element (14) arranged between the first and the second compartment (11, 12) having a plurality of openings (141) for fluid-conducting connection between the compartments (11, 12, 13) and a closable access opening for adding and removing mineral nutrient medium and peat moss gametophytes into or from at least part of the reactor interior (RI).Furthermore, the container (10) comprises a gas distributor (15) arranged centrally in a lower region (II) of the reactor interior (RI), with a plurality of gas openings for supplying gas into at least a part of the reactor interior (RI) along a gassed sub-region of a photobioreactor cross-sectional area, and a gas outlet (16) arranged in an upper region (I) of the reactor interior (RI) for removing exhaust gas from the container (10). The invention further relates to a method for the submerged production of peat moss, which is carried out using a photobioreactor (1) according to the invention.

This invention discloses a simple and rapid breeding method for artificially synthesizing wheat. It involves inducing incomplete meiosis in *Gnaphalium affine*, a close relative of wheat, under high red light (R / FR≥1.4) to produce unmeiotic divisions. This method falls under the field of crop breeding technology and includes the following steps: *Gnaphalium affine* (DD) cultured in a high red light (R / FR≥1.4) environment undergoes incomplete meiosis during reproductive growth to produce unmeiotic gametes. These gametes are then used as the male parent and crossed with *Langdon* (AABB). The resulting F1 generation (ABDD) is tetraploid. The F2 generation obtained by self-pollination of this F1 generation is hexaploid (AABBDD). Furthermore, the artificially synthesized hexaploid wheat offspring can be stably inherited.

The application provides a kind of industrialized breeding method of Undaria pinnatifida seedling, and belongs to the technical field of seedling breeding.The application provides an industrialized breeding method of Undaria pinnatifida seedling, comprising the following steps: respectively propagating female and male gametophytes of Undaria pinnatifida to obtain female and male gametophytes for inoculation; mixing the female and male gametophytes for inoculation, inoculating to the seedling curtain, and carrying out seedling culture.The industrialized breeding method of Undaria pinnatifida seedling of the application is suitable for industrialized breeding of Undaria pinnatifida seedling in high temperature areas, and has the advantages of short cycle, low cost and high quality of seedling.

This invention discloses a multi-layer vertical seedling device and method for kelp. The seedling device includes a seedling frame and several layers of seedling trays. The height of the seedling trays is adjustable. A dynamic shading curtain is installed at the top of the seedling frame. A supplemental lighting unit, a flow control unit, and an environmental monitoring unit are installed on each seedling tray. Normal seedling cultivation is performed on the first layer of seedling trays. In the early stage of seedling attachment on the second layer of seedling trays, white light is emitted by the supplemental lighting unit of the second layer to promote the attachment and germination of zoospores to form gametophytes. Subsequently, the supplemental lighting unit of the second layer of seedling trays emits red and blue light, with the proportion of red light being greater than that of blue light. During the extended cultivation stage on the second layer of seedling trays, moderate light intensity red light is used as the main source and blue light as a supplement to slow growth while ensuring the accumulation of photosynthetic products, increasing the chlorophyll content of seedlings, enhancing adhesion, reducing diseases and seedling rot, and improving the stability and yield of seedlings.

The invention discloses a plant pollen wall development related protein OsEXA5 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence of SEQ ID NO.1, is related to plant pollen wall development and is derived from SEQ ID NO.1. The plant pollen development related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene can lead to abnormal development of plant male gametophytes and influence the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterile transgenic plants can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

The invention discloses plant male sterility related aldehyde oxidase EXA1 as well as a coding gene and application thereof. The protein provided by the invention is a protein as shown in (a) or (b): (a) a protein consisting of an amino acid sequence as shown in SEQ ID NO.1; and (b) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO.1, is related to plant aldehyde oxidase and is derived from SEQ ID NO.1. The plant aldehyde oxidase related protein provided by the invention affects the male gametophyte development process of plants. The inhibition of the expression of the protein coding gene leads to abnormal development of plant male gametophytes and influences the formation of grains, so that transgenic plants with abnormal pollen wall development and male sterility can be cultivated. When the coding gene of the protein is introduced into a male sterile plant, a plant with normally developed male gametophyte can be cultivated.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

This invention provides a method for inducing and culturing embryogenic callus from *Pinus kesiya*, relating to the field of biotechnology. The method includes: collecting male cones of *Pinus kesiya* and performing surface disinfection on the collected male cones; immersing the surface-disinfected male cones in a detoxification solution for detoxification; washing the detoxified male flower explants and drying them; removing the dried explants, separating the male gametophytes from the explants, and culturing them in the dark in a culture medium until callus is induced. This method uses male gametophytes of *Pinus kesiya* for explant cultivation. Anthers, as reproductive organs of plants, possess stable genetic information, which helps maintain the superior traits of *Pinus kesiya*. Using *Pinus kesiya* anthers as breeding material allows for early and stable segregation of offspring, improving the reliability and accuracy of selection. Furthermore, the callus induced by pollen can maintain the superior traits of the maternal parent, which is of great significance for subsequent propagation of superior plants.