Method for quickly breeding pteris vittata by spore semi-tissue culture method

A technology of centipede grass and spores, which is applied in the field of plant tissue culture, can solve the problems of affecting the production rate of spore plants and unsatisfactory effects, and achieve the effects of mass reproduction, high survival rate and increased reproduction coefficient

Inactive Publication Date: 2012-07-18
SUN YAT SEN UNIV
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Problems solved by technology

And at present, the method that utilizes centipede grass spores to propagate centipede grass is also few, and the Chinese patent that application number is 200510086732.8 discloses a kind of method utilizing centipede grass spores to carry out rapid tissue culture propagation, mainly adopts specific culture medium to induce centipede grass For spore germination, the invention found that when different culture media are used, the production rate of spore plants will be greatly affected. When MS medium is used, the production rate is only <10%, and the effect is not ideal

Method used

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  • Method for quickly breeding pteris vittata by spore semi-tissue culture method

Examples

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Embodiment 1

[0021] Example 1 Aseptic treatment of centipede grass spores

[0022] Take the mature centipede grass spores, with 0.1% HgCl 2 As a disinfectant, disinfect for 1, 2, 4, 6, 8 minutes; and use 5% NaClO as a disinfectant, disinfect for 3, 6, 8, 10, 12 minutes, and then rinse 4-8 times with sterile water to obtain Sterile spore suspension. The sterile spore suspension under the above treatment was inoculated on the MS basic medium, the culture temperature was 20-25 °C, the light intensity was 1500-3000 lx, the light time was 12-14 hours / day, and the staining of each treatment was observed after 14 days. bacteria situation. The experimental results show that with 0.1% HgCl 2 Disinfection for 4-8 minutes or disinfection with 5% NaClO for 8-12 minutes, the infection rate is lower than 20%. When 0.1% HgCl 2 Disinfect for 6 minutes or disinfect with 5% NaClO for 10 minutes, and the bacterial contamination rate is the lowest, less than 10%.

Embodiment 2

[0023] Example 2 Germination of centipede grass spores

[0024] The centipede grass spores sterilized under the above optimal conditions were inoculated into different proportions of MS medium (MS, 1 / 2 MS, 1 / 5 MS, 1 / 10 MS, no MS), under different light intensities ( 1000, 1500, 2000, 2500, 3000 lx) and different light times (8, 10, 12, 14, 16, 18 hours / day), observe the bacterial contamination and spore germination of each treatment after 14 days. The results showed that green dots visible to the naked eye appeared on these mediums in 3 to 5 days, indicating that the spores had germinated, and the germination rates under different culture conditions all reached more than 90% (30 spores were randomly inoculated on the medium, and the spores were inoculated within 5 days. The proportion of green spots appearing on the medium to the total number of inoculations), but the germination rate is slightly higher than other conditions under the light intensity of 2000-2500 lx and ligh...

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Abstract

The invention discloses a method for quickly breeding pteris vittata by a spore semi-tissue culture method, comprising the following steps of: sterilely treating the spore of the pteris vittata, culturing on a culture medium to form into gametophyte, and seeding the gametophyte in soil to be cultured into the young seedling of the pteris vittata; the process for forming the gametophyte comprises the following steps of inoculating the sterile spore of the pteris vittata onto a 1/2 MS culture medium for cultivation at the temperature of 22-28 DEG C, periodically irradiating in the process of cultivation under the condition that the light intensity is 2000-2500lx, irradiating for 12-14 hours every day, and cultivating 5-8 days, i.e. germinating the spore to form into the gametophyte. The process for cultivating the gametophyte into the young seedling of the pteris vittata comprises the following steps of soaking the gametophyte in a 1/10 MS culture medium for 0.5-1.5 hours, seeding on the surface of soil, carrying out surface coating, and cultivating under the irradiation condition of natural light for 3-5 days. According to the breeding method, the pteris vittata breeding period can be shortened, the operation steps can be simplified, the pteris vittata is high in survival rate, and the breeding coefficient can be greatly improved.

Description

technical field [0001] The present invention relates to a kind of plant tissue culture method, more specifically relate to utilizing centipede grass ( Pteris vittata L.) Spores, a method of rapid seedling propagation by semi-tissue culture. Background technique [0002] centipede grass ( pteris vittata L.) is a perennial herbaceous plant of the genus Pteridaceae in the family Pteridaceae. It is mainly distributed in the tropical and subtropical regions south of the Qinling Mountains in China. It is born in roadsides, rock crevices or limestone mountains. It is calcareous soil or Limestone indicator plants. The whole herb is used in folk medicine, which can dispel wind, kill insects, and treat malnutrition; it can also be potted for ornamental purposes. Since it was discovered in 2001 that Centipede Grass has innate resistance and hyperaccumulation ability to arsenic, its potential value in scientific research and remediation of water or soil heavy metal pollution has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 周密杨兵廖斌李金天束文圣
Owner SUN YAT SEN UNIV
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