Quick reproduction of Anzu flower by cell embryo induction

A technology of somatic embryos and anthurium, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of unsuccessful reports of anthurium, and achieves short reproductive cycle, stable heredity, and proliferative quantity. big effect

Inactive Publication Date: 2004-12-08
SHANXI ACAD OF AGRI SCI GARDENING RES INST
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  • Abstract
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  • Application Information

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Problems solved by technology

Although the rapid propagation method induced by somatic embryos has been applie

Method used

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  • Quick reproduction of Anzu flower by cell embryo induction
  • Quick reproduction of Anzu flower by cell embryo induction
  • Quick reproduction of Anzu flower by cell embryo induction

Examples

Experimental program
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Effect test

Embodiment 1

[0044] The method for inducing rapid propagation of somatic embryos of Anthurium, which includes the following steps: take the young stem of Anthurium and cut the young stem into 3 mm long, insert it into the induction medium in a sterile state, and cultivate it in the dark at a temperature of 25°C for 40 The first day, the induced somatic embryos were transferred to the morphological growth medium formula, the temperature was 25-27°C, cultured under light, the light intensity was 3000Lx, 12h / d, and the complete plants were formed after 50 days of culture; the induction medium formula It is: 1 / 2MS+2.4-D1mg / L+TDZ0.05mg / L+inositol 25mg / L+casein 25mg / L+sucrose 30g / L; the morphological growth medium formula 1 / 2MS+KT0.05mg / L+ TDZ0.05mg / L.

[0045] The induced somatic embryos can be excised from the young stems and transferred to the morphological growth medium; the somatic embryos induced from the young stem slices are less, and the whole young stem slices can be transferred to the...

Embodiment 2

[0047] The method for inducing rapid propagation of somatic embryos of Anthurium, which includes the following steps: take the young stem of Anthurium and cut the young stem into 7 mm long, insert it into the induction medium in a sterile state, and cultivate it in the dark at a temperature of 27°C for 50 The first day, the induced somatic embryos were transferred to the morphological growth medium formula, the temperature was 27°C, cultured under light, the light intensity was 3000Lx, 16h / d, and cultured for 60 days to form a complete plant; the induction medium formula was: 1 / 2MS+2.4-D2.5mg / L+TDZ0.5mg / L+inositol 100mg / L+casein 100mg / L+sucrose 40g / L; the morphological growth medium formula 1 / 2MS+KT0.5mg / L+ TDZ 0.5mg / L.

Embodiment 3

[0049] The method for inducing rapid propagation of somatic embryos of Anthurium, which includes the following steps: take the young stem of Anthurium and cut the young stem into 5 mm long, insert it into the induction medium in a sterile state, and cultivate it in the dark at a temperature of 26°C for 45 On the first day, the induced somatic embryos were transferred to the morphological growth medium formula, the temperature was 26°C, cultured under light, the light intensity was 3000Lx, 14h / d, and cultured for 55 days to form a complete plant; the induction medium formula is: 1 / 2MS+2.4-D1.8mg / L+TDZ0.27mg / L+inositol 65mg / L+casein 65mg / L+sucrose 35g / L; the morphological growth medium formula 1 / 2MS+KT0.27mg / L+ TDZ0.27mg / L.

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Abstract

A fast reproduction method of Anzu flower by inducing somatic cells includes such steps as picking up the young stem of Anzu flower, cutting by 3-7 mm in length, inoculating in the inducing culture meidum under aseptic condition, culturing at 25-27 deg.C in dark condition for 40-50 days to induce embryo of somatic cell, and culturing at 25-27 deg.C under 2000 LX (12-16 hr per day) for 50-60 days. Its advantage is fast speed.

Description

technical field [0001] The invention relates to a rapid plant propagation technology, in particular to a method for inducing rapid propagation of anthurium somatic embryos. Background technique [0002] Anthurium is native to the tropical rainforests of Central and South America, and it is a rare flower introduced to my country in recent years. Anthurium has become a popular high-end flower because of its unique flower shape, gorgeous flower color, long vase life, annual flowering, and high ornamental value. In the global tropical flower trade, the sales volume of anthurium ranks second only to orchids. Anthurium can be used not only for cut flowers to improve the overall quality of flower arrangements, but also for potted flowers for decoration. In recent years, due to the continuous cultivation of new multi-flowered varieties, the demand for its potted plant market is rising sharply. [0003] There are mainly two kinds of existing anthurium propagation methods. One is ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 康黎芳曲复宁王云山柳亚林曹冬梅郭建民刘琛彬张超
Owner SHANXI ACAD OF AGRI SCI GARDENING RES INST
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