337results about How to "Short reproductive cycle" patented technology

An integrated process for the production of ethanol from woody plant material is provided, the process comprising: contacting a continuous flow of the plant material with a counter-current continuous flow of an aqueous ethanol solution at elevated temperature and pressure to provide plant material depleted of lignin; removing ethanol from the lignin-depleted plant material; contacting a continuous flow of the lignin-depleted plant material with water at elevated temperature and pressure to solubilize xylose within the plant material; and hydrolyzing cellulose present in the plant material to form glucose, which in turn is fermented to produce ethanol.

An integrated process for the production of ethanol from woody plant material is provided, the process comprising: contacting a continuous flow of the plant material with a counter-current continuous flow of an aqueous ethanol solution at elevated temperature and pressure to provide plant material depleted of lignin; removing ethanol from the lignin-depleted plant material; contacting a continuous flow of the lignin-depleted plant material with water at elevated temperature and pressure to solubilize xylose within the plant material; and hydrolyzing cellulose present in the plant material to form glucose, which in turn is fermented to produce ethanol.

The invention provides a method for raising seedlings of camellia chrysantha plants by top grafting. By the aid of the method, study for supporting technologies for raising the seedlings of the camellia chrysantha plants by top grafting is carried out in the aspects such as scion, rootstock selection and processing, the grafting time, culture conditions and matters needing attention, and in order to achieve purposes of increasing a survival rate and cultivating excellent strains with both ornamental value and medicinal value, a plurality of key links are improved in an emphasized manner and include that (1), large tea-oil trees at tree ages of 60-80 years are selected as rootstocks; (2), scion cuts are subjected to wax sealing processing in a scion storage period; and (3), as the rootstocks are large and are high in drying degree and crowns of the rootstocks are large, an independent spatial protection measure is adopted after grafting, in other words, protective covers are manufactured according to sizes of the trees. The method has the advantages that problems of low quantity of existing wild camellia chrysantha, extremely low fruit rate, long seedling stage duration, low emergence rate and the like in the prior art are solved, the reproductive cycle of camellia chrysantha can be shortened, seedlings can be yielded from a garden in a period ranging from half a year to a year, the survival rate can reach 95% at least, and the excellent strains with both the ornamental value and the medicinal value can be cultivated within the shortest time.

The invention relates to a method for producing sweet potato detoxified seedlings in a water culture manner. Healthy and robust detoxified seedlings are obtained by adopting an improved MS minimal medium and a greenhouse hydroponic micropropagation method. The requirements of soilless cultivation ways based on vermiculite, pearlite matrix and the like are met, so that healthy and efficient sweet potato seed production is carried out. By adopting the method, the original characteristic that the tissue culture seedling is free from viruses, bacteria and insects is kept, the traditional breeding technique is innovated, the growth speed of the detoxified seedlings is greatly accelerated, the breeding cycle is shortened, and the production cost is greatly reduced. The water culture seedling is simple in operation procedure, and the hydroponic seedlings can be produced within 4-6 months in a large scale according to the production requirements. Therefore, the problem that the detoxified seedlings cannot be quickly at low cost in a large scale by the traditional seedling cultivation method is solved.

The invention discloses a reproduction method of Chinese cymbidium seedlings, belonging to an orchid seedling reproduction technology, in particular to a reproduction technology for raising seedlings by tissue culture and mycorrhizal fungi intergrowth culture. In the reproduction method of the Chinese cymbidium seedlings, seedling acquisition mainly comprises the steps of seed germination, protocorm successive proliferation and rhizogenesis culture. The reproduction method of the Chinese cymbidium seedlings is characterized by comprising the following steps: carrying out hardening on rooted seedlings and the process of mycorhiza on mycorhiza fungi after the seedlings are obtained. By adopting the mycorhiza culture technology of sterile germination and mycorhiza fungi intergrowth of Chinese cymbidium seeds, the invention has the advantages that excellent variety can be bred, excellent Chinese cymbidium plants can be selected from the seedlings, the Chinese cymbidium breeding speed can be rapidly improved and the defects that pure tissue culture seedlings grow slowly, have long period and even do not bloom can be overcome, thus the invention has an important utilization value on large-area industrial reproduction for the Chinese cymbidium.

The invention relates to an orchid germchit propagating method, belonging to an orchid seedling propagating technology, in particular to a propagating technology for seedlings cultured based on tissue culture and mycorrhiza fungi symbiosis. In the orchid germchit propagating method, the germchit can be obtained in the steps of seed germination, successive multiplication of protocorm and rooting culture. The method is characterized in that the rooted seedling can be further acclimatized and the mycorrhiza fungi are mycorrhized after the germchit is obtained by using the propagating method. By using the mycorrhized culture technology based on sterile germination and mycorrhiza fungi symbiosis of the orchid seed, fine varieties can be propagated, fine orchid plants can be selected from the seedlings of seeds, and the propagating speed of the orchid can be quickened, thereby overcoming the defects of slow growth, long cycle and even nonflowering of the pure tissue culture seedling, and having important utility values in the large-area industrialized propagation of the orchid.

The invention discloses a chrysanthemum one-step tissue culture quick breeding method. The method comprises the following steps of S1, obtaining aseptic tissue culture seedlings, collecting chrysanthemum explants, cutting the chrysanthemum explants into sections with buds and stems, sterilizing, cleaning, inoculating the sections to a culture medium to grow, transferring the sections to a new culture medium to grow after the new buds grow to be 2 to 3cm, and obtaining the aseptic robust maternal seedling with roots; and S2, one-step quick breeding, cutting the aseptic robust maternal seedlingobtained in S1 into sections with buds and stems, inoculating the sections to the culture medium to carry out passage and rooting culture, and enabling the sections with buds and stems to grow into complete plants. The method has the beneficial effects that the chrysanthemum tissue culture seedlings with roots do not need special seedling training, the robust growth is realized, the production cost is low, the genetic property of the obtained tissue culture seedling is uniform, the breeding coefficient is high, the breeding period is short, and the method is an effective path for the chrysanthemum tissue culture quick breeding; the culture medium of the tissue culture and the culture medium of the robust seedlings and roots belong to the same type of culture medium, so the operation difficulty is reduced, the culture efficiency is improved, and the application range is wide.

The invention discloses a Tilia tomentose seed presprouting method. The method comprises the following steps: selecting fully mature new seeds, picking off the seed vessel mechanically firstly, carrying out quick burning and immersion treatment, then immersing the seeds in gibberellin and cytokinin, and finally carrying out presprouting through a temperature changing lamination method adopting a warm temperature before a low temperature. The method brings about a short breeding cycle and a high sprouting rate of Tilia tomentose. The ideal sprouting rate can be achieved for about 23 days. Compared with the conventional presprouting method, the presprouting method greatly shortens the time needed by the presprouting of the seeds to achieve the aim of the rapid breeding of Tilia tomentose, and solves the problem of seed resource waste caused by the low seed sprouting rate of Tilia tomentose.

The invention discloses a grape cuttage seedling method, which comprises the following steps of (1) soil preparation and ridge making; (2) rooting agent selection; (3) material selection and cuttage; and (4) management after cuttage and transplanting. When the cuttage seedling method provided by the invention is adopted, good properties of grape mother strains can be maintained, and the method has the advantages that the propagating factor is high, the plant growth is healthy, strong and regular, the operation is simple and convenient, and the like. The seedling propagating number is improved by more than 80 percent through being compared with that of a root burying seedling method, in addition, the popularization and the application are easy, the cost is low, the propagating period is short, and the seedling growth is fast.

The invention discloses an artificial aphid propagation method. Peas are utilized as a host plant to culture aphids and collect, select and store the propagated aphids. The artificial aphid propagation method realizes the large-scale, low-cost and standardized production of the aphids, makes the annual production of the aphids be possible and provides sufficient fodder or adding ingredients of artificial fodder for the growth and the propagation of natural enemy insects.

The invention relates to a method for aseptically producing miniature seed stems of common bletilla pseudobulb seeds. The method comprises the following steps of disinfecting the common bletilla pseudobulb seeds; germinating the seeds on a germination culture medium; inducing the seeds to root; and inducing artificial seed stems to form, grow and mature and the like. According to the method, technical routes and key technologies from the step of germinating the common bletilla pseudobulb seeds to the step of forming the mature seed stems are optimized and proposed, and a technology of culturing common bletilla pseudobulb tissues can be simplified, so that the common bletilla pseudobulb seeds are directly are bred in a large scale, and the common bletilla pseudobulb seed stems are produced. The germination rate of embryo seeds reaches more than 95 percent, and more than 80 percent of tissue culture seedlings can form mature miniature seed stems. According to the method, the difficulty that the common bletilla pseudobulb seeds rarely germinate under natural conditions is overcome, and the defects that the survival rate of transplantation of the seedlings in the conventional common bletilla pseudobulb tissue culture and seed aseptical breeding is low and the like are overcome. A feasible practical technology is provided for quickly producing a great amount of seed stems in factories by means of the common bletilla pseudobulb seeds, the current situation of the shortage of the seed sources of common bletilla pseudobulb can be alleviated, and large-scale artificial cultivation of the common bletilla pseudobulb is promoted.

The invention provides and relates to the technical field of plant tissue culture, and provides a tissue culture rapid propagation method of polygonatum multiflorum. The method sequentially comprisesthe following steps of performing sand storage; performing soaking by warm water being 35 to 40 DEG C; performing gibberellin treatment; performing pretreatment on the polygonatum multiflorum seeds soas to break the seed dormancy; inoculating the pretreated seeds into a seed cluster bud inducing culture medium; directly inducing the seeds into cluster buds, wherein the seed cluster bud inducing culture medium is prepared from 1.5 to 2.5mg / L of MS culture media 6-BA, 0.5 to 1.5mg / L of GA3, 6.5 to 7g / L of agar and 30 to 35g / L of sucrose, and the pH value is 5.5 to 6.0; dividing the cluster budsand performing propagation culture so as to expand the cluster bud quantity; performing rooting culture and domestication transplanting to obtain polygonatum multiflorum stock seedlings. The method provided by the invention has the advantages that the dormancy of the polygonatum multiflorum seeds can be effectively broken; the seed germination stage is shortened to five months; the effects of short propagation period and germination rate improvement by 85 to 90 percent are achieved.

The invention relates to a flower culture method, in particular to a breeding method for an oriental lily seedball. Middle and outer layer scales of the oriental lily seedball are peeled off to be stored for 4 to 6 hours in shade environment, then, the scales are completely soaked into disinfection reagent for disinfection treatment for 20 to 30 minutes, and then, the scales are taken out and drained; and the disinfected scales are completely soaked into hormone mixing solution to be treated for 10 to 15 minutes, are taken out and drained and are stored for 2 to 3 hours in shade environment, perlite and turf soil are mixed according to a volume ratio being 1 / 1 and are uniformly stirred to be used as a substrate, the substrate is subjected to the disinfection treatment, and then, the humidity is maintained at 60 to 70 percent. The lily scales are uniformly placed onto the substrate for covering the substrate for 3 to 5cm in a way that the concave surfaces of the lily scales are upward, a substrate embedding of the oriental lily scales is realized, the embedded lily scales are subjected to temperature change treatment, and the open field culture can be carried out. The breeding seedball is subjected to the temperature change treatment in a refrigeration house, and the breeding method has the characteristics that the condition is controllable, the operation is simple, the breeding period is short, and the like.

The present invention discloses a method for rapid in-vitro propagation of a Crassulaceae plant. The method comprises the steps of carrying out aseptic germination of seeds, inducing adventitious bud, and carrying out in-vitro rooting of the adventitious buds. The method allows in-vitro germinated seedlings to normally grow and to be induced within a short period of time to continuously differentiate from original single-seed single seedlings into single-seed multiple seedlings, so the propagation cycle is greatly shortened, and the growth cycle of the differentiated seedlings is greatly shortened. The method provides a stable technical platform for improvement of the breeding efficiency of high-quality varieties of the Crassulaceae plant.

The invention discloses a micropropagation method of fraxinus rhynchophylla and relates to the micropropagation method. The problems of long reproductive cycle, low reproduction efficiency and poor offspring genetic stability in nursery stock propagation of fraxinus rhynchophylla are solved. The method comprises the following steps of: 1, sterilizing fraxinus rhynchophylla seeds, and culturing single cotyledons of zygotic embryos to obtain cotyledonal cell embryos; 2, performing maturation cultivation on the cotyledonal cell embryos; 3, performing sprouting cultivation on the cotyledonal cellembryos subjected to maturation cultivation to obtain regenerated plants; and 4, transplanting the regenerated plants into a culture medium to culture until new leaves are completely unfolded, and removing a covered plastic thin film. The micropropagation method has the advantages of short nursery stock reproductive cycle, high reproductive rate (culturing for 60 to 70 days) and high reproductionefficiency (each explant can generate 15 to 20 somatic embryos); the germination rate of the somatic embryos is up to 87 to 89.55 percent; the transplanting survival rate of the regenerated plants isup to 75 to 80 percent; and the regeneration plants with the same good characteristics as female parents can be generated in mass.

The invention discloses a growth promotion cultivation method of fritillary bulbs. The growth promotion cultivation method mainly comprises the steps of selection and disinfection of explants, material treatment, primary culture, subculture, proliferation of bulblets, rooting culture, and management of soil for acclimatization and transplanting. Different hormones are used for adjusting at different culture phases; a formula of a culture medium is sieved accurately to realize the good effect of vigorous growth of test-tube seedlings; an effective sterilization technology is combined to achieve the aims of improving the proliferation rate, reducing variation, increasing soil fertility and reducing plant diseases and insect pests. The culture medium is replaced properly in the proliferation process of the bulblets which are subjected to subculture so as to facilitate the rapid growth of the bulblets. By comparing different parts of flakes, a best flake reproduction material is the lower part of inner-layer flakes.

The invention provides a method for breeding trichogramma japonicun ashmead by means of beet armyworm ovums. The method is relatively high in production efficiency and short in breeding period, and is beneficial to scale production of trichogramma japonicun ashmead. The method comprises following steps of 1) producing beet armyworm ova; and 2) breeding trichogramma japonicun ashmead by means of the beet armyworm ova. The trichogramma japonicun ashmea bred artificially are used for controlling agricultural pests which mainly comprise rice-stem borer, yellow rice borer and rice leaf roller. Compared with the prior art, the trichogramma japonicun ashmead artifical breeding production method is advantaged in that beet armyworm can be obtained easily, and can be bred simply; and the method is relatively high in production efficiency and short in breeding period, and is convenient for large-scale breeding, and is beneficial to scale production of trichogramma japonicun ashmead.

The tissue culture seedling growing process for trichosanthes includes the following steps: 1. taking the bud on tuberous root or stalk of trichosanthes as the explant in summer end or initial summer; 2.cutting bud or stem segment; 3. inoculating the bud or stem segment in culture medium I inside culture bottle; 4. secondary culture in culture medium II inside culture bottle; and 5. rooting culture in culture medium III inside culture bottle for 20-30 days to root. The present invention has short propagation period and high propagation coefficient.

The invention discloses a tissue culture method of North American begonia. The tissue culture method comprises the steps of primary culture, subculture, rooting culture and acclimatization for transplant, wherein current-grown tender branches of the North American begonia are taken as explants and are cultured on a primary culture medium for a period of time through sterile inoculation, then the seedlings of the North American begonia on the primary culture medium are cut and then inoculated on a subculture medium for subculture, clustered seedlings growing to be 2cm-3cm are transferred to a rooting culture medium for rooting induction so as to form rooting seedlings, and the rooting seedlings are subjected to hardening and acclimatization to obtain regeneration plants. According to the method, the North American begonia can be bred more easily, a large quantity of sterile seedlings can be obtained in a short time, and the breeding cycle is shortened, so that the planting area can be enlarged rapidly.

The invention discloses a method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings. The method comprises the following steps of: (1) material culture, (2) material disinfection, (3) explant bud induction, (4) subculture multiplication and culture, (5) virus detection, (6) rooting culture, and (7) seedling hardening and transplantation. According to the principle that the quantity of the viruses at the growing point at the top of a plant is far less than the quantity at the lower part of the plant, the generation number of taking the growing point is enlarged, so that the aim of detoxification is achieved. According to the method provided by the invention, low hormone concentration is adopted, the MS basic culture medium is improved and a three-day dark culture method is used, so that the aberration rate of the detoxification tissue culture seedlings is greatly reduced, the growth rate of the tissue culture seedlings is accelerated, the reproductive cycle is shortened, the expanding propagation speed is high and the production cost is low.

The invention relates to a tissue culture and rapid propagation method of epimedium wushanense and propagated epimedium wushanense. The method comprises the following steps of: A) performing stratification on epimedium wushanense seeds at low temperature of 0-10 DEG C after disinfection; B) performing disinfection treatment on the seeds after stratification, and stripping out seed embryos with sterile tweezers for later use; C) inoculating the seed embryos after disinfection into an induction culture medium for culture, and enabling the seed embryos to induce calli and further differentiate adventitious buds or enabling the seed embryos to directly induce buds; D) transferring the buds obtained in the step C) into a propagation culture medium, and generating tissue culture cluster buds; and E) transferring small plants grown from the cluster buds obtained by induction and differentiation in the step D) into a rooting culture medium, culturing to form white fibrous roots, and further forming complete plants. According to the method disclosed by the invention, the propagation coefficient of the epimedium wushanense can be improved, the propagation period can be shortened, and the method further has important values for protecting wild epimedium wushanense resources and promoting sustainable utilization and industrialization of the epimedium wushanense.

The invention discloses an artificial propagation method of esox reichertii, belongs to the cultivation filed of the esox reichertii, and is mainly used for solving the technical problems that the existing natural egg-laying process of the wild esox reichertii is unfocused in egg-laying time, long in egg-laying period, non-synchronous in maturity of male and female parent fishes and low in hatching rate. The artificial propagation method of the esox reichertii comprises the following steps of: sorting parents; and carrying out artificial impregnation after injecting the medicine twice by S-GnRH (gonadotropin releasing hormone)-A, HCG (Human Chorionic Gonadotropin) or DOM (Dimethoxy Mephentermine) in April, so that the artificial propagation of esox reichertii is completed. The artificial propagation method of the esox reichertii disclosed by the invention can be used for enabling the maturity of male and female parent fishes to be synchronous, enabling the egg-laying time to be focused and shortening the egg-laying period by 15-20 days, and also has a fertility rate of 85%-90% and a hatching rate of 60%-70%. The parent fishes are propagated artificially, the propagation success rate of male fishes is 97% and the propagation success rate of the female fishes is 70%-85%.

The present invention relates to a micropropagation method of purple-leaf birch, and relates to a micropropagation method. The invention settles the problems of long breeding cycle, long propagation efficiency, high cost and low survival rate. The method of the invention comprises the following steps: 1. sterilizing the explant; 2. cutting the explant to stem segment, and then executing induction cultivation for obtaining micro-branch; 3. cutting the micro-branch to stem segment and proliferating in the culture medium; and 4. executing invigoration culture to the proliferated micro-branch, sticking into a rooting substrate for executing rooting culture for finishing the micropropagation of purple-leaf birch. The micropropagation method of purple-leaf birch of the invention has the advantages of short propagation period, high efficiency and excellent genetic stability. The propagation efficiency is that the purple-leaf birch propagates for 30-40 times every 30-40 days. The method of the invention also has the advantages of simple operating technique, labor saving, time saving and low cost. The cost is saved by 30%-50% compared with the prior art. Furthermore the rooting rate and survival rate of seedling propagation equally can obtain 100%.

The invention relates to the field of plant protection and an insect breeding technology, in particular to a single-end feeding method of agriphila aeneociliella. The method comprises the steps that a single insect breeding case is adopted to serve as a breeding tool, and entangled cocooning and cannibalism among larvae can be avoided; mass propagation of pathogenic microorganisms such as bacteria and viruses can be effectively inhibited, and bacterium and virus transmission among insects is avoided; degerming and cleaning are conducted conveniently at any time, and a disinfection measure is adopted; timely replacing on feed is facilitated, and group consistency is kept; sterile vermiculite with the moisture of 20% serves as a pupa cell making medium, mature larvae are put on the medium surface, and pupating of the larvae and adult eclosion are facilitated.

The invention discloses a method for carrying out asexual cuttage propagation on vernicia fordii, belonging to the technical field of asexual propagation of plants. The method comprises the following steps of: (1) branch collecting time; (2) seedbed preparation and cuttage environment; (3) selection of cuttings and treatment of cutting branches; (4) pretreatment of a growth regulator; (5) cuttage; and (6) management after cuttage. The method provided by the invention is simple and feasible, has the advantages of low cost and short cycle and can conduce to realization of effective and rapid asexual propagation of the vernicia fordii. The method has the beneficial effect of solving the problem that the vernicia fordii can not root during cuttage, and has very important effects on and significance for accelerating popularization of excellent clone of the vernicia fordii and promoting construction of biodiesel raw material forests.

The invention discloses an ilex crenata golden gem tissue culture breeding method which comprises steps of sterilization, primary culture, secondary culture, strong seedling culture, rooting culture, seedling exercising and transplanting. Through the ilex crenata golden gem tissue culture breeding method disclosed by the invention, the breeding coefficient of ilex crenata golden gem can be improved, the breeding speed of the ilex crenata golden gem can be speeded up, the survival rate of the ilex crenata golden gem can be increased, technical support is provided for rapid breeding of excellent ilex crenata golden gem seedlings, and the ever-increasing demand of the market on the ilex crenata golden gem can be satisfied.

The invention discloses an acer negundo aurea tissue culture rapid propagation method which belongs to the technical field of plant tissue culture, and includes explant sterilization, starting culture, primary culture, subculture and transplanting steps. Only one medium is used in the method, and the medium is 1 / 2DKW+0.1mg / L NAA (naphthalene acetic acid) +0.05mg / L IAA (indole acetic acid) + 30g / L sucrose + 5g / L agar, proliferation and rooting culture can be performed on the same medium, and the method has the characteristics of being simple in culture process, easy to operate, high in propagation coefficient, short in breeding cycle, free of hardening, and high in survival rate.

The invention relates to a novel method for measuring the biological activity of biological pesticides, which belongs to a novel method for measuring the biological activity. The novel method is characterized in that an agaragar layer is taken as a short-time cultivation nutrient source and a moisturizing material of a plant excised part in the measurement of biological activity; and meanwhile, green peach aphids are adopted as biological detecting materials to detect the biological activity of a detecting sample, and the insecticidal activity measurement is carried out according to the average reproduction quantity and the average death rate of the green peach aphides and the relevance of biological pesticides to be detected. The method solves the problems that plant leaves are dried easily and dehydrated generally in the insect bioassay by utilizing excised plant leaves to further influence the bioassay result and improves the accuracy of the experimental result. The method has simple and convenient operation, short experimental period and low cost. The invention can be applied to the biological activity measurement of biological pesticides and other pesticides of current varieties and the screening researches of new pesticides and is especially suitable for the bioassay of biological pesticides to insects with sucking mouth parts, such as aphides, and the like.

A fast reproduction method of Anzu flower by inducing somatic cells includes such steps as picking up the young stem of Anzu flower, cutting by 3-7 mm in length, inoculating in the inducing culture meidum under aseptic condition, culturing at 25-27 deg.C in dark condition for 40-50 days to induce embryo of somatic cell, and culturing at 25-27 deg.C under 2000 LX (12-16 hr per day) for 50-60 days. Its advantage is fast speed.