Method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings

A technology for tissue culture seedlings and sweet potatoes, applied in the field of agricultural biology, can solve the problems of viral disease spread, slow propagation speed and high production cost, and achieve the effects of shortening the breeding cycle, accelerating the growth speed and low production cost.

Inactive Publication Date: 2013-07-17
INST OF FOOD CROPS HAINAN ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

However, due to the widespread existence of sweet potato virus diseases, and the asexual reproduction of sweet potatoes in production, the virus diseases spread, resulting in reduced yields, quality declines, and seed degradation of sweet potatoes.
At present, in scientific research, virus-free seedlings are mainly obtained through in vitro culture of shoot tips, but this method is applied to industrialized mass production, and the propagation speed is slow, the mutation rate is high, and the production cost is high.

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  • Method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings

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Embodiment Construction

[0027] The method for efficient industrialized production of sweet potato virus-free tissue culture seedlings of the present invention comprises the following steps:

[0028] 1. Material cultivation

[0029] Retrieve the 20-30 cm long stems of sweet potatoes to be detoxified from the field, and cut them on the seedbed of the greenhouse to let them grow new branches. During the process, pour the nutrient solution once a week, and spray the leaves and the surrounding environment with 50% carbendazim (or chlorothalonil) 500 times every 5 days. The environment inside the shed is clean.

[0030] When the new branch grows to 8-10cm, cut off the new branch, replant it to a new substrate, and let it grow new branches in the same way.

[0031] 2. Material disinfection

[0032] When the new branch of the second cutting seedling grows to more than 8cm, take two or three sections of the stem, cut it off, put it in a sterilized airtight glass bottle and seal it, take it to the workbench...

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Abstract

The invention discloses a method for efficiently and industrially producing sweet potato detoxification tissue culture seedlings. The method comprises the following steps of: (1) material culture, (2) material disinfection, (3) explant bud induction, (4) subculture multiplication and culture, (5) virus detection, (6) rooting culture, and (7) seedling hardening and transplantation. According to the principle that the quantity of the viruses at the growing point at the top of a plant is far less than the quantity at the lower part of the plant, the generation number of taking the growing point is enlarged, so that the aim of detoxification is achieved. According to the method provided by the invention, low hormone concentration is adopted, the MS basic culture medium is improved and a three-day dark culture method is used, so that the aberration rate of the detoxification tissue culture seedlings is greatly reduced, the growth rate of the tissue culture seedlings is accelerated, the reproductive cycle is shortened, the expanding propagation speed is high and the production cost is low.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for efficient industrial production of virus-free sweet potato tissue-cultured seedlings. Background technique [0002] Sweet potato ( Ipomoea batatas Lam. ) is an important food, feed and industrial raw material crop, which has the advantages of high yield, rich nutrition and good yield stability. However, due to the widespread existence of sweet potato virus diseases and the vegetative propagation of sweet potatoes in tubers during production, the virus diseases spread, resulting in reduced yield, lower quality and degeneration of sweet potato species. At present, virus-free seedlings are mainly obtained through in vitro culture of shoot tips in scientific research. However, this method is applied to industrial mass production, and the propagation speed is slow, the mutation rate is high, and the production cost is high. Contents of the invention...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 朱红林王效宁刘迪孟卫东齐兰
Owner INST OF FOOD CROPS HAINAN ACAD OF AGRI SCI
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