1308 results about "Culture tissue" patented technology

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The invention discloses a potato cold resistance identification method and system, and relates to the technical field of cold resistance identification. The method comprises the following steps: cultivating potato tissue culture seedlings in a standardized manner, and randomly dividing the seedlings into a low-temperature stress group and a normal-temperature control group; performing standardized low-temperature treatment including slow descent and constant-temperature stages on the stress group; the method comprises the following steps: acquiring multi-source data such as chlorophyll fluorescence parameters, canopy thermal imaging, digital image phenotypes and biochemical indexes, and forming a unified data set after derivative index calculation and standardized fusion; inputting the fusion data into an improved neural network model, training the model through a particle swarm optimization algorithm, and outputting a quantitative potato cold resistance index; automatically evaluating the cold resistance grade according to the potato cold resistance index and generating an identification report; standardization of the identification process, fusion analysis of multi-dimensional indexes and intelligent result analysis are achieved, and the accuracy, efficiency and practicability of potato cold resistance identification are remarkably improved.

The invention provides a tissue culture method and an acclimatization and transplantation method of coptis chinensis, and belongs to the technical field of plant tissue culture. The tissue culture method of the coptis chinensis comprises the following steps: (1) taking a tender shoot of a coptis chinensis rhizome as an explant, sterilizing, and inoculating into a basic culture medium for culturing, so as to obtain a coptis chinensis sterile seedling; (2) inoculating the aseptic seedlings of coptis chinensis into an adventitious bud differentiation culture medium for culture to obtain adventitious buds of coptis chinensis; (3) inoculating the coptis chinensis adventitious buds into a proliferation culture medium for culturing to obtain coptis chinensis proliferation seedlings; and (4) inoculating the coptis chinensis multiplication seedlings into a rooting culture medium for culture to obtain coptis chinensis test-tube seedlings. The tissue culture and acclimatization and transplantation method provided by the invention can improve the propagation efficiency of the coptis chinensis, remarkably shorten the growth cycle of the coptis chinensis, provide technical support for rapid propagation and sustainable utilization of resources of the coptis chinensis, and have wide application prospects.

The disclosure provides seed and plants of the iceberg lettuce cultivar designated cv. NNS-2109. The disclosure thus relates to the plants, seeds and tissue cultures of iceberg lettuce cv. NNS-2109, and to methods for producing a lettuce plant produced by crossing a plant of iceberg lettuce cv. NNS-2109 with itself or with another lettuce plant, such as a plant of another line. The disclosure further relates to seeds and plants produced by such crossing. The disclosure further relates to parts of a plant of iceberg lettuce line cv. NNS-2109, including the gametes of such plants.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to rice endophytic sphingosine vector SY02 and application thereof. The preservation number of the rice endophytic sphingobium yanoikuyae SY02 provided by the invention is CCTCC (China Center For Type Culture Collection) NO: M2026182, and the sphingobium yanoikuyae SY02 has the functions of producing indoleacetic acid, promoting plant growth and seed germination, promoting adventitious bud proliferation of tissue culture seedlings and relieving browning. The sphingosine vector bacterium and the microbial agent prepared through strain fermentation have the advantages of being green, safe, efficient, long-acting and the like, and have good application prospects in the aspects of promoting plant growth and seedling breeding.

The invention discloses an application of an NtMADS21 gene in regulation and control of tobacco traits. The nucleotide sequence of the NtMADS21 gene is shown as SEQ ID NO. 1. The invention also discloses an application of the NtMADS21 gene in regulation and control of tobacco traits. Through overexpression of the NtMADS21 gene, it is found that the NtMADS21 gene can synergistically improve the plant type and the tissue culture character of tobacco, specifically, the plant height is reduced, the number of leaves is increased to shape an ideal plant type, the leaf callus induction capacity is remarkably improved to 78.5%, in addition, the NtMADS21 gene has the effect of delaying flowering, and the yield of tobacco is increased. The invention provides a key gene resource integrating various excellent characters for genetic improvement and efficient breeding of tobacco.

The invention specifically discloses a salix cheilophila in-situ genetic transformation method independent of a tissue culture system, and belongs to the field of plant genetic engineering. According to the method, semi-lignified branches of salix cheilophila are taken as receptors, vacuumizing treatment is performed by utilizing agrobacterium rhizogenes infection liquid, hairy roots are directly induced under a water culture condition after short-term dark culture, and positive identification is performed through reporter genes. According to the method, tedious sterile tissue culture operation is completely avoided, the transformation period is shortened to about 22 days, and the technical threshold and the time cost are remarkably reduced. By adopting the system, the positive rate can reach 54.33% when the pBI121 vector is used for transformation, and the efficiency is remarkably improved compared with that of an existing method. The method provides an efficient and reliable technical platform for root system gene function research, expression analysis and genetic improvement of salix cheilophila and related species thereof, and has high application potential.

The invention provides a method for extracting ginseng tissue culture adventitious root saponin by a eutectic solvent synergistic biological enzymolysis technology, which comprises the following steps: (1) adding ginseng tissue culture adventitious root dry powder into water, adding compound enzyme, heating, adjusting the pH value of the solution, and carrying out biological enzymolysis; after the enzymolysis is finished, performing enzyme deactivation and centrifugation to obtain supernate A and precipitate; (2) adding an eutectic solvent into the precipitate, and performing ultrasonic extraction and centrifugation to obtain supernate B; and (3) combining the supernate A and the supernate B, and freeze-drying to obtain the ginseng tissue culture adventitious root saponin. According to the method, the biological enzymolysis technology and the eutectic solvent extraction technology are combined, the content of total saponins in the ginseng tissue culture adventitious root extract is increased, and meanwhile the content of Rg3, Rg5 and Rh2 rare ginsenosides in the ginseng tissue culture adventitious root extract is also increased. The invention belongs to the technical field of plant component extraction.

This invention discloses a method for exogenous rooting of Albizia julibrissin tissue culture seedlings, comprising the following steps: disinfection of explant material, propagation culture of tissue culture seedlings, external insertion of tissue culture seedlings into bottles, and post-insertion management, to obtain exogenously rooted Albizia julibrissin tissue culture seedlings. This method combines rooting and transplanting acclimatization in the rooting stage of tissue culture seedlings, improving the survival rate of tissue culture seedlings and accelerating the propagation process of Albizia julibrissin seedlings.

This invention discloses a method for rapid seedling cultivation of Eucommia ulmoides via tissue culture, relating to the field of Eucommia ulmoides breeding technology. The method includes the following steps: using young, tender stem segments with axillary buds from superior Eucommia ulmoides strains as explants, after surface disinfection, the segments are cut into sections with axillary buds and inoculated into an axillary bud induction medium to induce axillary bud germination; the germinated axillary buds are cut into segments and inoculated into a specific medium for adventitious bud induction and elongation culture; the adventitious buds after proliferation culture are separated and subjected to a two-step in-bottle rooting method or external adventitious root induction, ultimately obtaining complete regenerated Eucommia ulmoides plants. Beneficial effects: This invention develops a simplified Eucommia ulmoides tissue culture propagation method that shortens the production cycle, has a high propagation coefficient, rapid rooting, high adventitious root induction rate, and minimal basal callus. It can rapidly obtain high-quality Eucommia ulmoides seedlings, which is of great significance for the large-scale propagation of superior strains and varietal improvement research, while also providing technical support for the sustainable development of the Eucommia ulmoides industry.

The invention provides seeds and plants of spinach hybrid SVVB2741. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2741 and to methods for producing a spinach plant produced by crossing such plants with themselves or with another plant, such as a spinach plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of spinach hybrid SVVB2741 comprising introduced beneficial or desirable traits.

The invention relates to the technical field of forestry machinery, in particular to planting equipment for transplanting tilia amurensis tissue culture seedlings, which comprises a moving trolley, a guide rod, adjusting frames, lifting frames, a material frame, a planting hopper and the like, the guide rod is connected to the middle of the front side of the moving trolley, the adjusting frames are symmetrically connected to the guide rod in a sliding manner, and the lifting frames are connected to the adjusting frames in a sliding manner; a material frame is installed on the lifting frame, two planting hoppers are arranged at the positions, corresponding to the position under the material frame, of the lifting frame, and the two planting hoppers on the corresponding sides are designed to be closed oppositely. The downward movement of the planting hopper enables the water injection barrel to generate negative pressure and automatically suck and store water from the liquid storage barrel through the cooperation of the piston rod and the positioning block, after the planting hopper is lifted and opened to release the saplings, the water injection barrel resets to press the stored clear water into the material frame and irrigate the clear water to the roots of the saplings, and the design integrates the two procedures of planting and irrigation into one and automatically completes the two procedures. And water can be immediately supplemented after planting.

The invention provides seeds and plants of pepper hybrid SVPH8928, pepper line SLR-8T20-6755, and pepper line SLR-8T18-6570. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of pepper hybrid SVPH8928, pepper line SLR-8T20-6755, and pepper line SLR-8T18-6570 and to methods for producing a pepper plant produced by crossing such plants with themselves or with another plant, such as a pepper plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of pepper hybrid pepper hybrid SVPH8928, pepper line SLR-8T20-6755, and pepper line SLR-8T18-6570 comprising introduced beneficial or desirable traits.

The invention discloses a strain NJSH56 for promoting plant growth and application thereof, the strain NJSH56 is identified as bacillus pumilus, the strain NJSH56 is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation time is November 14, 2024, and the strain preservation number is CGMCC NO.32614. The strain NJSH56 can promote plant growth. The dendrobium officinale endophytic growth-promoting strain NJSH56 for promoting plant growth is obtained through separation and screening, growth of dendrobium officinale tissue culture seedlings can be effectively promoted, and the fresh weight, the rooting number and the leaf number are increased; greenhouse tests show that the number of new buds of dendrobium officinale plants in a strain NJSH56 treatment group reaches 4.67 and is obviously higher than that of a control group; meanwhile, the NJSH56 is used for treating the non-host plant Chinese cabbage, so that the growth of the overground part of the Chinese cabbage can be remarkably promoted, and the biomass accumulation of the Chinese cabbage is improved.

The invention belongs to the technical field of plant tissue culture, and particularly relates to an induction method of adventitious roots of cinnamomum camphora. According to the method disclosed by the invention, the adventitious root induction rate of the adventitious root of the cinnamomum camphora is remarkably improved by establishing a sterile system of the cinnamomum camphora, culturing cluster buds and sterile seedlings, promoting rooting of tissue culture seedlings, inhibiting browning, enabling the adventitious buds to extend and inducing the adventitious root through three ways, so that the problems of shortage of borneol resources and high market demand are solved; the method for inducing the adventitious roots of the cinnamomum camphora can be used for industrial production, and sufficient raw materials are provided for extraction of borneol; according to the method, large-scale culture of the adventitious roots of the cinnamomum camphora is achieved, a large number of pharmaceutical raw materials can be obtained at low cost in a short time, and an integrated production system can be formed with the pharmaceutical industry. The technology has important practical significance for promoting the development of the cinnamomum camphora industry, expanding the application of the pharmaceutical field, constructing a rare and endangered traditional Chinese medicinal material breeding technology system and realizing sustainable development and utilization.

The invention discloses a method for in-vitro preservation of common wild rice and Nwa wild rice, and belongs to the technical field of plant tissue culture. The method comprises the following steps: selecting a stem node with an axillary bud as an explant; soaking in 75% alcohol for 10-15 seconds, soaking in 10% 84 disinfectant for 15 minutes, performing vacuum treatment in 0.1% mercuric chloride solution under-0.1 Mpa for 5 minutes, and performing water bath at 40 DEG C for 20 minutes for combined sterilization; inserting the sterilized explant into a bud differentiation culture medium containing 2mg / L KT and 0.1 mg / L NAA, and culturing for 3-5 days under the conditions that the temperature is 23 DEG C and illumination is performed for 16 hours per day; cutting off buds growing to 3-5cm, and transferring the buds into a rooting culture medium; (5) carrying out test-tube plantlet propagation through stem node shearing or tillering cutting; the tissue culture seedlings with well developed root systems are hardened and then transplanted to a mixture of vermiculite and nutrient soil (v / v = 1 / 1). According to the method, the pollution rate is as low as 16.35%, the differentiation rate of the explant reaches 47.36%, the process is simple, convenient and efficient, the production period is short, the seed quality resources of common wild rice and Nilval wild rice can be effectively preserved, and the method has important significance on protection of precious wild rice resources.

The invention discloses a lilium brownii tissue culture process, which comprises the following steps: selecting a newly-grown and tender lilium brownii stem section part, cutting off the stem section part as an explant, inoculating the explant into an inoculation culture medium for inoculation culture, and carrying out induction starting culture on the explant / lateral bud obtained after inoculation culture; the method has the advantages that adventitious bud induction can be effectively carried out on the lilium brownii, the rapid propagation rate reaches 2.0-2.5, the seedling rate of strong seedling culture reaches 65%, and the rooting rate reaches 71.4%, so that the lilium brownii can adapt to large-scale production and follow up the market demand.

The invention discloses a culture medium composition and a culture method for in-vitro rapid propagation culture of hippeastrum hippeastrum, and belongs to the technical field of plant tissue culture. The adenine, the rhizoma polygonati and the wormwood extract are added into the hippeastrum striatum in-vitro rapid propagation culture medium for the first time, and the culture medium combination for hippeastrum striatum in-vitro rapid propagation culture is developed. The hippeastrum hippeastrum in-vitro rapid propagation culture method established on the basis of the culture medium combination can be used for in-vitro rapid propagation culture of different varieties of hippeastrum hippeastrum, the germination rate of primary culture in the hippeastrum hippeastrum in-vitro rapid propagation culture can be increased, and the differentiation coefficient and the seedling formation coefficient in the hippeastrum hippeastrum tissue culture process can be increased. The efficient and stable hippeastrum striatum in-vitro rapid propagation method established by the invention lays a technical foundation for accelerating a hippeastrum striatum breeding process and commercialized production.

The invention discloses a rubus cochinchinensis efficient genetic transformation method based on RUBY reporter gene and agrobacterium rhizogenes mediation, which realizes that the transformation period is shortened to be within 15 days and the transformation efficiency is improved to be more than 60% through direct explant treatment under a non-tissue culture condition. The method comprises the following specific steps: 1) preparing an explant material; (2) constructing an RUBY carrier and transforming agrobacterium rhizogenes; (3) infecting the explant by utilizing agrobacterium rhizogenes carrying a target gene; (4) co-culturing after infection and carrying out RUBY visual screening; 5) positive root molecular biology verification; (6) inducing positive roots into buds; according to the method, induction of the positive callus, the positive root and the positive bud of the rubus coreanus stem segment under the non-tissue culture condition is successfully achieved, a stable and efficient genetic transformation system is constructed, the method has the remarkable advantages of being easy and convenient to operate, low in cost, wide in applicability, high in reproducibility and the like, and a key technical support is provided for molecular breeding of the rubus coreanus.

The invention belongs to the technical field of medicinal plant tissue culture, and particularly discloses a culture medium for inhibiting browning of fructus amomi explants, the pH value of the culture medium is 5.0-6.0, the culture medium contains KNO3, NH4NO3, KH2PO4, MgSO4. 7H2O, CaCl2. 2H2O, MnSO4. 4H2O, ZnSO4. 7H2O, H3BO3, VB1, VB6, VB5, soybean mash filtrate, salicylic acid and the like, the formula of the culture medium aims at solving the problem of tissue culture browning of fructus amomi, and extremely low ammonium, organic nitrogen synergism and microelement regulation are adopted. By adopting the culture medium, the browning rate in the villous amomum fruit tissue culture process can be effectively inhibited, the seedling quality and the production efficiency are remarkably improved, the seedling culture period and the seedling culture cost can be greatly reduced in the villous amomum fruit seedling rapid propagation culture process, and a technical guarantee is provided for commercial seedling supply.

The invention discloses a high-yield and high-activity pear protoplast extraction method based on light shielding and enzymolysis optimization, which comprises the following steps: by taking pear tissue culture seedling leaves growing for 30-40 days or field pear tender leaves as an extraction material, carrying out enzymolysis treatment and protoplast purification by adopting enzymatic hydrolysate to obtain protoplast; before enzymolysis of the pear tissue culture seedlings, the whole pear tissue culture seedlings are placed in a dark environment and subjected to dark pretreatment at 25 + / -2 DEG C for 48-72 hours. According to the method, the yield of the field pear leaf and tissue culture seedling pear leaf protoplast can be increased by more than or equal to 2 times (the highest protoplast yield is greater than or equal to 3.0 * 10 / g FW), the activity is increased to be greater than or equal to 95%, and the technical bottleneck of the existing patent is remarkably broken through. The method has the advantages of high yield, high activity, wide application range and the like, can provide a high-quality protoplast material for pear genetic regeneration, gene editing, single cell transcriptome analysis and metabonomics research, and has wide scientific research and industrial application potential.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture and rapid propagation method for chamaecyparis obscura. Comprising the following steps: carrying out variable-temperature static electricity treatment on Arborvitae branches in North America; preparing composite particles for promoting seedling strengthening; carrying out induction culture and proliferation seedling strengthening on the explants subjected to variable-temperature electrostatic treatment; performing rooting culture on the compound strong seedlings, and performing acclimatization and transplanting. The method comprises the following steps: screening formulas of a sterilization mode, an induction culture medium, a proliferation culture medium, a strong seedling culture medium and a rooting culture medium, taking current-year branches of North America arborvitae as explants, sterilizing, sequentially putting the explants into the induction culture medium, the proliferation culture medium, the strong seedling culture medium and the rooting culture medium for culturing, and regulating and controlling optimal culture conditions, so as to obtain the explants. The germination induction rate of the explant is up to 92% or above, the multiplication coefficient is 5.0 or above, the rooting rate is 85% or above, the transplanting survival rate is 87% or above, a basis can be provided for large-scale propagation and variety improvement of North America juniperus seedlings, and the method has important value for fully developing North America juniperus resources.

The utility model provides an agilawood tissue culture protection device, and relates to the technical field of seedling raising trays. The protection assembly comprises a protection box, the seedling raising tray is arranged in the protection box, a plurality of first vertical plates are arranged in the protection box at equal intervals, the top of each first vertical plate is slidably connected with a second vertical plate, and the top end of each second vertical plate is supported on the lower surface between every two adjacent seedling raising holes of the seedling raising tray. Through the cooperation of the first vertical plate and the second vertical plate, the lower surface between the adjacent seedling raising holes of the seedling raising plate can be supported, the phenomenon that the seedling raising plate is broken due to the overlarge weight is prevented, then the seedling raising plate is protected, and the service life of the seedling raising plate is prolonged. Through sliding connection of the first vertical plate and the second vertical plate, the heights of the first vertical plate and the second vertical plate can be adjusted, and the seedling raising tray support is suitable for supporting seedling raising trays with different heights.

The invention discloses a method for extracting sphagnum moss protoplast, which comprises the following steps of: culturing a sphagnum moss material obtained by tissue culture under a certain condition, and performing pretreatment and enzymolysis under a specific permeation condition to finally obtain the high-quality sphagnum moss protoplast. The method provided by the invention provides an important technical basis for molecular biology research of sphagnum moss transgenosis and the like.

The invention discloses a method for inducing proliferation of paphiopedilum protocorm-like bodies by using oryzalin. Comprising the following steps: enabling paphiopedilum seeds to germinate in a germination culture medium to form buds; the buds are soaked and induced in the dark for 6-36 h, and an induction culture medium is a 1 / 2 MS liquid culture medium containing 0.5-5 mg / L of 2, 4-D, 0.05-1 mg / L of TDZ, 5-20 [mu] mol / L of oryzalin and 2% of dimethyl sulfoxide; inoculating the induced buds into a culture medium which does not contain oryzalin for culturing; carrying out proliferation culture on the protocorm-like body; carrying out differentiation culture on the protocorm-like body cluster; rooting and seedling strengthening culture; and transplanting rooted seedlings. According to the method disclosed by the invention, the sterile seeding buds of the paphiopedilum brevicornum protospecies are taken as the explants, the protocorm-like bodies are successfully induced, and the induction effect is remarkable. The research on differentiation, rooting, seedling strengthening and transplanting maintenance is systematically carried out, and a set of tissue culture and efficient rapid propagation technical system for paphiopedilum leucopeniculatum, which is low in cost, simple and convenient to operate and high in application value, is formed.

The application discloses application of SbWOX gene and SbBBM gene in corn breeding and a corn genetic transformation method, and relates to a bioengineering technology.The application provides a nucleic acid molecule with co-expression of SbWOX protein gene and SbBBM protein gene, and corresponding gene co-expression vectors and microbial transformants.The application also provides application of the co-expression of the SbWOX gene and the SbBBM gene in the corn genetic transformation method.The application obtains corresponding genes and mutant proteins based on the SbWOX gene and the SbBBM gene of sorghum, the two genes are co-expressed in the corn genetic transformation process, even without adding plant growth hormones in the antibiotic screening medium in the tissue culture process, the plant genetic transformation process can be normally completed, and the transformation efficiency and the regeneration efficiency of the corn can be remarkably improved, the transformation rate reaches 10.42%, and the differentiation rate reaches 32.89%.

The invention provides a cydonia oblonga primary and subculture tissue culture method, and belongs to the technical field of plant biology. According to the hormone combination ratio provided by the invention, the two core problems of low propagation coefficient and high seedling disease-carrying rate in the traditional propagation of the quince are fundamentally solved, and the propagation coefficient is stabilized by 10 times. The method has the advantages of being clear in process, high in repeatability, low in pollution rate, high in multiplication coefficient, robust in seedlings and the like, and an efficient and reliable technical means is provided for germplasm resource preservation and industrial development of quince.

The invention belongs to the field of plant tissue culture, and particularly relates to a method for promoting accumulation of camptothecin by overexpressing tryptophan synthetase. The invention provides a method for promoting camptothecin accumulation of camptotheca acuminate cells, which comprises the following steps: constructing camptotheca acuminate cells overexpressing tryptophan synthetase genes, performing tissue culture amplification, and extracting camptothecin from the amplified camptotheca acuminate cells. The method for overexpressing the tryptophan synthetase gene is superior to a method for directly adding tryptophan into a culture medium, and the camptothecin yield can be increased by 2.33 times.

The application discloses a culture medium for tissue culture of Limosella australis, which comprises a primary culture medium, a proliferation and differentiation culture medium and a rooting culture medium. The primary culture medium is MS+6BA 0.4-0.6 mg / L+NAA 0.04-0.06 mg / L+AC 0.8-1.2 mg / L+2,4-D 0.04-0.06 mg / L. The proliferation and differentiation culture medium is modified MS+6BA 0.09-0.11 mg / L+NAA 0.04-0.06 mg / L+AC 1.5-2.5 mg / L. The rooting culture medium is 1 / 2MS+IAA 0.09-0.11 mg / L+AC 2.5-3.5 mg / L. The technical scheme has the highest callus induction rate of 86.7%, the highest differentiation rate of 86.7% and the highest rooting rate of 93.3%.

The invention provides an open type tissue culture method for plica rugosa, which comprises the following steps: S1, performing disinfection treatment on a plica rugosa explant to obtain a pretreated explant; s2, the pretreated explant is inserted into a first modified MS culture medium for first-stage culture, and a first-stage explant is obtained; s3, transferring the first-stage explant into a second modified MS culture medium for second-stage culture to obtain a second-stage explant; s4, transferring the second-stage explant into a third modified MS culture medium for third-stage culture to obtain a primary seedling. According to the method, cell-free fermentation filtrate and PPM are sequentially and progressively added into a three-stage modified MS culture medium, so that high-efficiency culture of the explant of the plica rugosa in an open environment is realized, the pollution rate and the browning degree are reduced, meanwhile, healing of the explant, adventitious bud differentiation and root primordium formation are promoted, and the seedling rate of open tissue culture of the plica rugosa is increased.