224 results about "Culture tissue" patented technology

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

The utility model belongs to the technical field of citrus cultivation, and particularly relates to a citrus polyploidy efficient induction device which comprises a machine body. A sealing door is rotationally arranged on the side wall of the machine body; the sealing doors are symmetrically arranged on the side wall of the machine body; an induction processor is mounted on the side wall of the machine body; the output end of the induction processor is connected with a built-in discharger; the built-in discharger is arranged on the inner side wall of the machine body; a spraying opening is formed in the side wall of the built-in discharger; the spraying openings are formed in the side wall of the built-in discharger in a linear array mode. A storage rack is fixedly connected to the interior of the machine body; the storage racks are symmetrically arranged in the machine body; an embedding groove is formed in the side wall of the storage rack; by means of the structure, tissue culture is not needed by adopting a living body induction technology, multi-time growth can be achieved from seed germination to regeneration, compared with a traditional in-vitro induction method, the method is more convenient, and multi-time cultivation of citrus can be achieved in a sealed state.

This invention discloses a method for establishing a high-efficiency leaf regeneration system for aseptic seedlings of Populus tomentosa 'Bofeng No. 3', belonging to the field of agricultural biotechnology. This invention, through 5-AU combined with dark culture treatment, not only induces early leaf differentiation in aseptic seedlings of Populus tomentosa 'Bofeng No. 3', but also significantly increases the number of adventitious buds and the rooting rate. Through chemical treatment and optimized culture conditions, this invention successfully established a high-efficiency plant regeneration system for aseptic leaves of Populus tomentosa 'Bofeng No. 3', laying the foundation for the genetic transformation and gene editing of Populus tomentosa 'Bofeng No. 3', and also providing a reference for the establishment of tissue culture systems for other tree species.

The invention provides seed and plants of watermelon line TML-EJ19-3004. The invention thus relates to the plants, seeds, and tissue cultures of watermelon line TML-EJ19-3004 and to methods for producing a watermelon plant produced by crossing such plants with themselves or with another watermelon plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of watermelon line TML-EJ19-3004 comprising introduced beneficial or desirable traits.

The utility model discloses a flower tissue culture device with sunshade structure belongs to flower tissue culture related technical field to solve the prior art in the need of sunlight irradiation, generally can only through the external device shielding or will tissue culture device to be carried to the position that sunlight cannot irradiate, but when the flower needs part sunlight when tissue culture, and the flower tissue culture device can not make a part of sunlight irradiation on the device, this reduces the working effect problem of flower tissue culture device, including frame, the top surface and two lateral surfaces of frame are seted up with the sliding slot, a plurality of sliding slot are linked with corresponding frame body sliding respectively, a plurality of sliding slot are linked with corresponding baffle sliding respectively, and the surface of a plurality of baffles is contacted with corresponding frame body respectively.

The present application belongs to the field of agricultural biotechnology, and relates to a Hainan Catimor coffee tissue culture breeding method, which takes the middle and upper section of the hypocotyl of a Hainan Catimor coffee sterile seedling as an explant, carries out, after sterile treatment, five stages of callus induction, adventitious bud differentiation, cluster bud proliferation, rooting culture and seedling raising and transplanting in turn; each stage adopts a modified MS culture medium, and is matched with a special exogenous hormone combination, and meanwhile, the culture temperature is controlled to be 20-25 DEG C, the suitable light intensity and light time are controlled, and a specific concentration of PPM is added to inhibit pollution. The present application has the advantages of fast induction speed, stable culture effect, compact and non-deformed callus, healthy and neat adventitious buds and cluster buds, effective reduction of the proportion of abnormal seedlings and weak seedlings, significant improvement of the in-vitro regeneration efficiency and tissue culture seedling quality of Hainan Catimor coffee, simple operation, strong repeatability, suitability for large-scale factory breeding of Hainan Catimor coffee, and important application value.

The present invention provides novel snap pea cultivar Sugar 204 and plant parts, seed, and tissue culture therefrom. The invention also provides methods for producing a pea plant by crossing the pea plants of the invention with themselves or another pea plant. The invention also provides pea plants produced from such a crossing as well as plant parts, seed, and tissue culture therefrom.

According to the disclosure, there is provided seed and plants of the corn variety designated CV968805. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV968805, and to methods for producing a corn plant produced by crossing a corn plant of variety CV968805 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV968805 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV968805.

The present disclosure relates to cell culture vessels and methods of using the same that are capable of providing a laminar flow of cell culture media to cells in the cell culture vessels. The cell culture vessels include a plurality of wells in which cells reside as media moves across the top thereof. The cell culture system is further enclosed to maintain sterility. The media can be constantly renewed within the cell culture vessel, can be recirculated within the cell culture vessel, or a combination of both. Circulation of media around the cells residing therein can be particularly useful for 3D tissue culture, such as for producing spheroids or organoids.

The invention provides seed and plants of pea line SVQF0546. The invention thus relates to the plants, seeds, and tissue cultures of pea line SVQF0546 and to methods for producing a pea plant produced by crossing a plant of pea line SVQF0546 with itself or with another pea plant, such as a plant of another line. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of a plant of pea line SVQF0546, including the seed, pod, and gametes of such plants.

The application discloses a tissue culture and rapid propagation method for Yangmei of Alocasia macrorrhiza and a special combined culture medium thereof, and the method comprises the steps of sterilizing an explant, carrying out primary culture and subculture and proliferation culture in stages, wherein the primary culture is performed by first inoculating a stem segment into an induction culture medium containing 6-BA 0.8-1.2 mg / L, IBA 0.05-0.15 mg / L and PVP-40 to induce germination, and then transferring the stem segment into an elongation culture medium containing 6-BA 0.1-0.5 mg / L, NAA 0.01-0.05 mg / L and PVP-40 to promote elongation; the new shoots are then transferred into a subculture medium containing 6-BA 1.0-1.8 mg / L, NAA 0.1-0.2 mg / L, GA3 0.5-1.5 mg / L and PVP-40 to proliferate. The application effectively solves the technical problems that Yangmei is prone to brown and death, and it is difficult to simultaneously achieve budding and high growth by means of two-stage hormone stepwise regulation and cooperation with a brown prevention agent, and realizes efficient and large-scale breeding of high-quality seedlings.

The utility model relates to the field of crop tissue culture technology, and disclose crop tissue culture sterilization device, including support spare and sterilization spare, the support spare includes the horizontal placement base plate, the base plate top is provided with the rotation of the vertical to horizontal plane axis rotation's rotation plate, both ends of rotation plate all are fixedly connected with the support ring, be provided with the placement groove on the support ring, the inside of placement groove places the detachable storage spare, the inside of storage spare forms the placement cavity of placement culture medium, and storage spare top open forms the through -hole, and the through -hole is communicated with the placement cavity, the utility model discloses through rotatable rotation plate drive support ring and storage spare rotation, and then let one of the through -hole on the storage spare and the gas outlet cover communicate, make the device to culture medium sterilization, personnel can place culture medium in another storage spare simultaneously, realize feeding and sterilization can be carried out simultaneously, let the device can work continuously, improve the sterilization efficiency of device.

The invention discloses a rapid propagation rooting culture medium for peony seed tissue culture and a culture method, and belongs to the technical field of plant tissue culture. The rapid propagation rooting culture medium takes 1 / 2 MS as a basic culture medium, and further comprises the following components: ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, monopotassium phosphate, a carbon source, kinetin and a curing agent. By adding kinetin into the culture medium, dependence of peony seed tissue culture seedlings on low-temperature treatment is broken, low-temperature pretreatment necessary in a traditional method is avoided, the peony seed tissue culture process is greatly simplified, the culture period is shortened, the rooting rate and the root strengthening rate are increased, the rooting rate is stabilized to be 95% or above, and the rooting rate is stabilized to be more than 95%. Technical support is provided for establishment of a new peony seed tissue culture and rapid propagation technology, and a material foundation is laid for promotion of peony seed tissue culture and rapid propagation factorization.

This invention discloses a rapid propagation method for Litsea cubeba tissue culture that effectively reduces browning and improves rooting rate. The method selects healthy, young stem segments of Litsea cubeba as explants, sterilizes them, and inoculates them into a primary induction medium to induce initial bud formation. These are then inoculated into a subculture proliferation medium to obtain subculture clusters of buds. Single buds are then cut and inoculated into a rooting medium for dark culture to induce rooting. Finally, the seedlings are hardened off and transplanted to obtain tissue culture seedlings ready for nursery. This invention solves the problems of low subculture proliferation coefficient and severe browning by optimizing the explant sterilization measures, the primary induction medium, the subculture proliferation medium, and the rooting medium formulation, reducing the explant browning rate to below 5.0%. Simultaneously, the subculture yields a large number of clustered buds in a short time with a high proliferation coefficient; the tissue culture seedlings have more than four roots, resulting in a high rooting rate; the transplant survival rate is high, and the seedling quality is good. This reduces the industrial-scale production cost of Litsea cubeba tissue culture seedlings and provides technical support for large-scale seedling production and artificial cultivation of Litsea cubeba.

The application belongs to the technical field of tissue culture and relates to a kind of induction method and application of monkey ear ring flower filament callus, comprising the following steps: taking monkey ear ring flower bud, after being treated with disinfecting liquid for disinfection and sterilization, in sterile environment, take monkey ear ring flower filament as explant, it is inoculated in induction medium and dark culture, form callus, then the callus is transferred to proliferation medium and is subcultured and proliferated, and more high-quality callus is obtained.The induction method provided by the application can induce monkey ear ring flower filament to form callus quickly, and the callus induction rate is as high as 100%;The induction method of monkey ear ring flower filament callus in the application has short cultivation period, high induction efficiency and low contamination rate, the induction method is applied to the cultivation of monkey ear ring, the research path of monkey ear ring rapid propagation technology is widened, the regeneration of monkey ear ring flower filament into plant becomes possible, and high-quality plant with excellent traits can be cultivated.

This utility model relates to the technical field of planting troughs for transplanting tissue culture seedlings of sugar beets, and more particularly to a planting trough for transplanting tissue culture seedlings of sugar beets. It includes a fixed base plate, with threaded adjusting rods rotatably connected to both sides of the top of the fixed base plate. A fixed top block is fixedly connected to the top of the threaded adjusting rod, and a threaded connecting block is threadedly connected to the outer wall of the threaded adjusting rod. Through the mechanical linkage design of the threaded rotating rod, traction plate, fixed sliding rod, and receiving drip plate, the non-destructive seedling removal function of sugar beet tissue culture seedlings is achieved. The rotating block drives the receiving drip plate to rise smoothly, pushing the seedling and root soil as a whole to the top of the planting cylinder. Operators can directly remove the seedlings without digging, reducing root damage compared to traditional methods. Field trials have verified that the survival rate of sugar beet tissue culture seedlings transplanted using this planting trough is significantly higher than that of traditional planting troughs, and the seedling recovery period is shortened, laying a good foundation for later growth and development, and significantly improving the economic benefits of sugar beet cultivation.

This invention relates to the field of plant tissue culture and the breeding of superior varieties of traditional Chinese medicinal materials. This invention discloses a method for rapid propagation of Epimedium inflorescences via tissue culture, comprising the following steps: S1, collecting newly sprouted flower inflorescences of Epimedium in early spring, disinfecting them, and setting them aside; S2, cutting the disinfected inflorescences into 1.1-1.2 cm segments, inoculating them into callus induction medium, and culturing them under conditions of 1200-1300 lux light intensity, 12 h / day photoperiod, and 24℃. This invention ensures uniform seedling quality and stable traits through targeted screening of effective components and monitoring of genetic stability. The optimized culture medium ratios at each stage result in a high proliferation coefficient, robust rooting, and a short propagation cycle, enabling efficient year-round propagation. Standardized seedling hardening and transplanting lead to a high survival rate. This method is technically stable and highly applicable, enabling the mass production of high-flavonoid glycoside, genetically stable, high-quality seedlings, fundamentally solving problems such as germplasm degradation, low propagation coefficient, and uneven quality, providing strong support for the breeding and industrialization of superior varieties of Epimedium.

The present application relates to the technical field of plant tissue culture, and specifically discloses a method for establishing a tissue culture sterile system of peach rootstock dormancy material, which comprises the following steps: S1, selecting one-year-old branches with leaf buds in the dormancy period, removing flower buds and cleaning; S2, performing low-temperature treatment on the branches, then performing water culture and bud breaking to obtain sprouting branches; S3, performing surface sterilization treatment on the sprouting branches, and then washing with sterile water; S4, peeling the sprouting buds from the branches under sterile conditions, removing the external wrapping tissues of the bud bodies, and exposing the internal tender tissues; S5, performing secondary sterilization treatment on the exposed tender tissues, and then washing with sterile water; and S6, inoculating the bud bodies subjected to the secondary sterilization treatment into a culture medium for culture, and establishing a sterile system; according to the above method, in the process of establishing the sterile system of Zhongtao Kangzhen No. 1 material, the sterilization survival rate of 100 bud heads is as high as 80% or more, and the work efficiency and the utilization efficiency of the material are greatly improved.

The application discloses a kind of wild membrane pod astragalus rapid induction of adventitious shoots, which is by tissue culture to select wild membrane pod astragalus sterile seedling young stem as explant, inoculation is added in MS culture medium of different concentrations 6-BA, IAA and IBA to induce adventitious shoots.Adjusting the ratio of different hormones, the best induction condition suitable for wild membrane pod astragalus adventitious shoots is screened out, a set of method suitable for wild membrane pod astragalus rapid induction of adventitious shoots is established, the method of wild membrane pod astragalus induction of adventitious shoots is enriched, and the method is high and can be used for the induction of a large number of adventitious shoots, greatly shorten the wild membrane pod astragalus seedling cycle and reduce cost, lay a good foundation for wild membrane pod astragalus germplasm resource protection and seedling rapid propagation.

A method is disclosed for synthesizing Ethephon-THAMs and using it as a controlled ethylene-releasing agent in potato tissue culture. The invention enables selective enhancement of shoot or root development and improves micropropagation efficiency

This invention discloses a method for virus-free tissue culture using terminal buds of *Prunus armeniaca* rootstock, belonging to the field of plant tissue culture technology. The method includes: selecting terminal buds of *Prunus armeniaca* rootstock for pretreatment, sterilizing them with ethanol and mercuric chloride, and then immersing them in a virus-free pretreatment solution containing ribavirin and triazole nucleoside for virus removal; inoculating the virus-free terminal buds into WPM-based differentiation induction medium to obtain clustered buds; culturing the clustered buds in 1 / 2 MS-based proliferation subculture medium to obtain proliferating buds; inoculating the proliferating buds into 1 / 3 MS-based rooting medium to induce rooting; and finally, after hardening off and transplanting into a vermiculite-garden soil-humus substrate for acclimatization. This invention optimizes the virus removal and tissue culture system based on the characteristics of *Prunus armeniaca* rootstock, achieving a virus removal rate ≥95%, a terminal bud differentiation survival rate ≥85%, a rooting rate ≥90%, and a transplant survival rate ≥88%. This enables rapid and large-scale propagation of virus-free *Prunus armeniaca* rootstock seedlings, solving the problem of low propagation efficiency in traditional methods and providing high-quality rootstock support for the *Prunus armeniaca* industry.

The application provides a Guizhou germplasm Dendrobium devonianum tissue culture rapid propagation method, which comprises the following steps: pre-treatment of explant material, sterile protocorm induction culture, cluster bud subculture proliferation culture, strong seedling rooting induction and seedling culture, and finally obtains Guizhou germplasm Dendrobium devonianum tissue culture seedlings. The application induces protocorms from Guizhou germplasm Dendrobium devonianum seeds, forms cluster buds through subculture proliferation, and obtains in-vitro regenerated plants through strong seedling rooting, seedling and transplanting, and establishes a complete tissue culture rapid propagation system. The method has the characteristics of high success rate, high induction rate, high proliferation coefficient, high genetic stability, good seedling quality, low cost, consistent growth, short seedling cycle and the like, and has universal applicability, and provides reliable technical support for mass breeding, rapid propagation and high-quality germplasm resource protection of Guizhou germplasm Dendrobium devonianum.

This invention provides a method for the germination and regeneration of mature rubber tree embryos into seedlings outside of test tubes, belonging to the field of seedling cultivation technology. The method uses somatic embryos derived from anther tissue culture as raw materials, and involves three stages of cultivation in a growth medium: dark culture, low-light culture, and strong-light culture. Then, the mature embryos that meet the requirements undergo closed-bottle / open-bottle acclimatization. After this acclimatization, they can be rooted and grown into seedlings in river sand outside the test tube. This method allows mature embryos to germinate and regenerate directly into seedlings outside the test tube, eliminating the need for a sterile culture room and specialized rooting medium, thus requiring less specialized knowledge from the operator. Furthermore, by combining the embryo germination and seedling regeneration with the in-test-tube and sand-bed acclimatization in one step, the cultivation process is simplified, the cultivation cycle is shortened by two months, and cultivation efficiency is improved while production costs are reduced.