3280 results about "Culture tissue" patented technology

According to the disclosure, there is provided seed and plants of the corn variety designated CV955045. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV955045, and to methods for producing a corn plant produced by crossing a corn plant of variety CV955045 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV955045 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV955045.

According to the disclosure, there is provided seed and plants of the corn variety designated CV960136. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV960136, and to methods for producing a corn plant produced by crossing a corn plant of variety CV960136 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV960136 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV960136.

According to the disclosure, there is provided seed and plants of the com variety designated CV644310. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV644310, and to methods for producing a corn plant produced by crossing a corn plant of variety CV644310 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV644310 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV644310.

According to the disclosure, there is provided seed and plants of the corn variety designated CV962284. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV962284, and to methods for producing a corn plant produced by crossing a corn plant of variety CV962284 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV962284 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV962284.

According to the disclosure, there is provided seed and plants of the corn variety designated CV978472. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV978472, and to methods for producing a corn plant produced by crossing a corn plant of variety CV978472 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV978472 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV978472.

According to the disclosure, there is provided seed and plants of the corn variety designated CV851273. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV851273, and to methods for producing a corn plant produced by crossing a corn plant of variety CV851273 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV851273 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV851273.

According to the invention, there is provided seed and plants of the corn variety designated CV554903. The invention thus relates to the plants, seeds and tissue cultures of the variety CV554903, and to methods for producing a corn plant produced by crossing a corn plant of variety CV554903 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV554903 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV554903.

According to the disclosure, there is provided seed and plants of the corn variety designated CV636724. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV636724, and to methods for producing a corn plant produced by crossing a corn plant of variety CV636724 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV636724 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV636724.

According to the disclosure, there is provided seed and plants of the corn variety designated CV963205. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV963205, and to methods for producing a corn plant produced by crossing a corn plant of variety CV963205 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV963205 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV963205.

According to the disclosure, there is provided seed and plants of the corn variety designated CV964538. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV964538, and to methods for producing a corn plant produced by crossing a corn plant of variety CV964538 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV964538 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV964538.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture and rapid propagation culture medium and a tissue culture and rapid propagation method for culturing Shaniodendron subaequalis. The invention provides a tissue culture and rapid propagation culture medium and a tissue culture and rapid propagation method for culturing Shaniodendron subaequalis. Kinetin KT (Kinetin) in the tissue culture and rapid propagation culture medium provided by the invention can promote the generation of buds and the growth of adventitious buds. 6-BA is 6-benayl aminopurine and is used for promoting the growth of buds; tDZ is thiabendazole, and growth and development of buds are promoted. Under the combined action of KT, 6-BA and TDZ, the bud induction rate and the bud multiplication coefficient of the stem with the bud are improved. The invention further provides a tissue culture and rapid propagation method of the Shaniodendron subaequalis, stem segments subjected to induction culture are low in pollution rate, high in survival rate and low in browning rate; the lateral bud induction rate of the stem section is as high as 91%, and the multiplication coefficient, the rooting rate and the transplanting survival rate are high.

According to the disclosure, there is provided seed and plants of the corn variety designated CV964750. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV964750, and to methods for producing a corn plant produced by crossing a corn plant of variety CV964750 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV964750 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV964750.

The invention discloses a Zhejiang camellia japonica clone tissue culture and rapid propagation method. A Zhejiang camellia japonica clone tissue culture and rapid propagation technical system is established by taking a non-lignified tender stem as an explant, sterilizing and then performing primary induction, subculture multiplication, strong bud culture and rooting culture. The explants are soaked before primary inoculation, and vitamin C and polyvinylpyrrolidone with certain concentration are added into a primary induction culture medium, so that browning of the explants can be effectively inhibited; hormone regulation and control and nutrient addition are combined, so that the primary induction rate reaches up to 95.3%, the multiplication coefficient reaches about 6.8, the rooting rate reaches 83.5%, efficient and rapid propagation can be realized, and the method is not limited by factors such as seasons, climate and regions. The rapid propagation offspring obtained by the system can maintain all excellent characters of a stock plant, and the strong bud obtained in the process can be used as a scion for grafting propagation, so that a technical support is provided for protection, development and utilization of rare germplasm resources and excellent clone of the Zhejiang red camellia.

According to the disclosure, there is provided seed and plants of the hybrid com variety designated CH010478. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010478, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010478 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010478.

The invention discloses a prothallus proliferation culture medium and a rapid propagation method for rare and endangered plant cibotium barometz. The prothallus proliferation culture medium of cibotium barometz is used for accelerating the proliferation of gametophytes, and the prothallus maturation time is shortened; meanwhile, the invention provides a rapid propagation method of cibotium barometz, which comprises the following steps: carrying out artificial sterile culture on cibotium barometz spores, rapidly germinating in a culture bottle to form prothalli, culturing on a prothalli proliferation culture medium, inducing and transforming gametophytes in a specific matrix outside the bottle to form sporophytes by virtue of an incomplete tissue culture method, and transplanting. According to the method, the formation of prothallium is accelerated, the sporophyte conversion rate is increased, the reproduction rate is increased while the domestication and reproduction of sporophyte seedlings are accelerated, a large number of cibotium barometz seedlings which grow healthily can be obtained, the survival rate reaches 90%, the spore breeding period is obviously shortened, the operation requirement is not strict, and the method is suitable for large-scale popularization and application. The technical problems that the seedlings in the tissue culture bottle are easy to pollute, fragile and low in seedling rate are solved.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010442. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010442, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010442 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010442.

Disclosed is an innovative method for genetic transformation through injection of Agrobacterium containing plasmid DNA expressing PLETHORAs (PLT5) or / and WOUNDINDUCED DEDIFFERENTIATION 1 (WIND1) in multiple plant species including Snapdragon and tomato. In addition, this plasmid has also been tested that it can significantly improve the genetic transformation method is not genotype dependent. Genetic transformation can be achieved by promoting de-novo regeneration of shoots in adult plants, which will of significant impact on some perennial plant species such as citrus and blue berry etc. The transgenic de-novo shoot regenerated from adult plants can by-pass the juvenile phase of plants regenerated through the tissue culture method. Expression of PLT5, WIND1, or both can also greatly improve plant regeneration and transgenic transformation through in-vitro tissue culture in recalcitrant plant species.

According to the disclosure, there is provided seed and plants of the hybrid com variety designated CH010517. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010517, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010517 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010517.

The invention relates to the technical field of plant tissue culture, in particular to a monstera deliciosa tissue rapid propagation method and application thereof.The monstera deliciosa tissue rapid propagation method comprises the steps that a monstera deliciosa near leaf petiole is selected, a cleaned explant is cut into small sections to be inoculated into a primary cluster bud induction culture medium for primary induction culture, and a monstera deliciosa tissue culture medium is obtained; carrying out subculture cluster bud multiplication culture after primary induction, then carrying out strong seedling culture, and finally carrying out rooting culture. According to the method, the pollution rate can be controlled within 45%, the disinfection injury and death rate of the explants can be controlled within 14%, the induction rate of primary cluster buds is 70% or above, the multiplication coefficient is stabilized at about 4.0, the rooting rate reaches 99.8% or above, and rapid propagation of monstera deliciosa is achieved.

According to the disclosure, there is provided seed and plants of the corn variety designated CV951892. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV951892, and to methods for producing a corn plant produced by crossing a corn plant of variety CV951892 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV951892 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV951892.

According to the disclosure, there is provided seed and plants of the corn variety designated CV622763. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV622763, and to methods for producing a corn plant produced by crossing a corn plant of variety CV622763 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV622763 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV622763.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a method for establishing an elymus nutans seed callus induction and regeneration system. The invention provides a tissue culture medium and a tissue culture method for culturing elymus nutans, and in the tissue culture medium provided by the invention, 2, 4-D can induce explants to form calluses and promote cell division and growth. The CH has a good effect on differentiation of embryoid and adventitious buds. ABA can promote the development of seed embryos. The KT can promote the generation of calluses and the growth of adventitious buds. NAA can promote plant growth and promote generation of adventitious roots. Under the combined action of NAA, 2, 4-D, ABA, KT and CH, the callus induction rate of the mature embryo is improved. The invention also provides a regeneration tissue culture method of elymus nutans, the mature embryo is used as an explant for tissue culture, and the callus induction rate of the mature embryo is high.

According to the invention, there is provided seed and plants of the corn variety designated CV859255. The invention thus relates to the plants, seeds and tissue cultures of the variety CV859255, and to methods for producing a corn plant produced by crossing a corn plant of variety CV859255 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV859255 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV859255.

The invention discloses a sugar-free tissue culture microenvironment intelligent control system for facility flower seedling culture, and belongs to the field of intelligent agriculture. The system comprises a microenvironment regulation device, an LED light supplement regulation device, a carbon dioxide concentration regulation device and the like, a digital model construction module and an agricultural Internet of Things intelligent control platform. Key elements such as temperature and humidity, illumination, carbon dioxide concentration and nutrient solution of the flower seedling culture microenvironment can be accurately adjusted, and the requirements of different growth stages of flowers can be met; by means of a digital model and sensor feedback, automatic management is achieved, remote monitoring and operation can be achieved, manual intervention is reduced, the resource utilization efficiency is improved, and resource waste is avoided. A microenvironment precise regulation and control technology is adopted, so that the thermal load and energy consumption are reduced, the operation cost is reduced, and the economic benefit is improved; practical application verifies that the system can promote healthy growth of flowers, significantly improve flower quality and seedling culture management efficiency, and enhance market competitiveness of the flower seedling culture industry.

The invention relates to the technical field of plant tissue culture, in particular to a Rhododendron pulchrum tissue culture method based on callus regeneration. According to the method, the tender cotyledons of aseptic seedlings are taken as explants, and an efficient Rhododendron pulchrum tissue culture and rapid propagation technical system is established through induction of calluses, subculture multiplication of the calluses, differentiation of adventitious buds, bud strengthening and rooting culture, so that complete tissue culture progeny plants are formed. The induction rate of the callus tissue of the system is 100.0%, the proliferation multiple of the callus tissue in a subculture cycle reaches 5.2 times, 28.5 adventitious buds can be differentiated from a callus tissue block with the size of about 0.5 mm * 0.5 mm * 0.5 mm, the rooting rate reaches 95.8%, the system is not limited by factors such as seasons, climate and regions, and efficient and rapid propagation can be achieved all the year round. Meanwhile, the establishment of the system also lays a technical foundation for genetic transformation improvement, mutant screening breeding and the like of the Rhododendron pulchrum based on the callus.

The invention relates to a high-frequency regeneration and rapid propagation method of Enkianthus chinensis tissue culture seedlings. The invention is characterized in that dormant axillary buds of semi-lignified young stems are used as the base. The method comprises the following main steps: sampling and disinfection treatment of explants, inducing of dormant axillary bud germination, inducing of adventitious bud and lateral bud high-frequency generation, strong seedling culture, test-tube rooting culture and acclimatization and transplanting. Finally, regenerative plants which have consistent parental character, excellent quality and strong stress resistance are obtained. According to the invention, lots of seedlings with consistent specification and excellent quality can be obtained within a short period of time, and marketization progress of Enkianthus chinensis is promoted powerfully.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010587. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010587, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010587 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010587.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010588. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010588, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010588 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010588.

According to the disclosure, there is provided seed and plants of the corn variety designated CV957366. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CV957366, and to methods for producing a corn plant produced by crossing a corn plant of variety CV957366 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to corn seeds and plants produced by crossing plants of variety CV957366 with plants of another variety, such as another inbred line. The disclosure further relates to the inbred and hybrid genetic complements of plants of variety CV957366.

According to the disclosure, there is provided seed and plants of the hybrid corn variety designated CH010461. The disclosure thus relates to the plants, seeds, and tissue cultures of the variety CH010461, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010461 with itself or with another corn plant, such as a plant of another variety. The disclosure further relates to genetic complements of plants of variety CH010461.