44results about How to "Genetic stability" patented technology

The invention belongs to the technical field of gene breeding, and discloses a rapid matching breeding improved parent composition, an obtaining method and application. According to the method, a basic SNP marker is obtained; carrying out GWAS analysis on the basis of the collected phenotypes, carrying out dominant genotype identification on markers associated with the phenotypes, and constructing a dominant genotype library of the functional sites; calculating the contribution degree PVE of the functional sites to phenotypes, analyzing the contribution degree PVE, calculating the linkage region Block of each material or group, and constructing a material PVE score library and a Block library; and matching and combining every two materials based on dominant genotypes, PVE of functional sites and linkage regions Blocks of the materials / groups to obtain an optimal combination parent of each material. According to the invention, more favorable genotype polymerization states can be realized.

The invention discloses a method for targeted creation of a fast-growing new tilapia strain, and belongs to the technical field of fish cultivation. The method specifically comprises the following steps: (1) taking a fragment, as shown in SEQ ID NO.1, on a third exon of a pomc gene as a gRNA target sequence; (2) designing and synthesizing a gRNA primer sequence; (3) amplifying by adopting the gRNA primer sequence and the gRNA plasmid, and then transcribing and purifying to obtain a gRNA fragment; (4) transferring the gRNA fragments into fertilized eggs of tilapia mossambica through gene editing to obtain F0-generation positive fish; and (5) hybridizing the F0-generation positive male fish with the wild-type female fish, screening out F1-generation parent fish, and culturing the F1-generation parent fish to obtain F2-generation tilapia with homozygous deletion mutation. According to the invention, the pomc homozygous knockout line is established in the Nile tilapia mossambica for the first time, the new fast-growing line is a complete gene function deletion model, the cost is lower, the efficiency is higher, and the phenotype can be stably inherited.

The invention belongs to the technical field of bioengineering, and provides a recombinant XVII type collagen as well as a preparation method and application thereof. A target gene sequence is obtained through bioinformatics predictive analysis and integrated design on the basis of a human XVII type collagen sequence, codon optimization is carried out, a T7 promoter in a plasmid vector is optimized, an engineering strain of the high-hydrophobicity humanized XVII type collagen is prepared, and the high-hydrophobicity humanized XVII type collagen is obtained. The strain is subjected to culture and inducible expression to obtain high-yield recombinant XVII type collagen. The recombinant XVII type collagen disclosed by the invention is reasonable in sequence design, large in expression quantity, simple in preparation method and stable in heredity, and rapid separation of the collagen can be realized in a production and preparation process by utilizing high hydrophobicity.

The invention discloses a mutant with improved enzyme activity of sucrose isomerase and application of the mutant, and belongs to the technical field of genetic engineering and enzyme engineering in molecular biology. A known sucrose isomerase parent is subjected to molecular modification by utilizing a site-directed mutagenesis technology to obtain mutant enzymes T522S / K340D, D222H / K340D, G467N / K340D, T522S, K340D, D222H and G467N, and the catalytic activity of the series of mutants provided by the invention is increased by 10%-25% compared with that of original bacteria; the isomaltulose conversion rates of the combined mutants T522S / K340D, D222H / K340D and G467N / K340D are respectively increased by 2.3%, 1.5% and 1.3% compared with those of original bacteria, and the strain has good stability, can be used for biological preparation of isomaltulose and has potential application prospects.

The invention discloses a japonica rice amphiphilic hybridization target gene plant screening method based on high-throughput sequencing, and belongs to the technical field of agricultural biology. A high-throughput sequencing technology is utilized, the genetic difference of parent genomes is accurately analyzed, specific molecular markers covering target gene areas are developed, plants containing target genes can be rapidly recognized, the screening efficiency and accuracy are greatly improved, the breeding period is shortened, resources are saved, and the method is suitable for large-scale popularization and application. Through combination of molecular marker detection and phenotypic expression of target characters, in the construction process of hybridization and backcross populations, target genes are fixed generation by generation, stable inheritance of the target characters, joint analysis of genetic types and phenotypes and multi-environment testing are ensured, correlation between target genotypes and the target characters and environmental adaptability of the target genotypes and the target characters are verified, and the target genotypes and the target characters are identified. The stability and reliability of the breeding material are improved, in candidate plant screening and restorer line creation, an excellent hybrid combination is constructed, genotype optimization is realized, and the quality and yield potential of the breeding material are improved.

The invention discloses recombinant bacillus subtilis with the surface displaying Edwardsiella tarda outer membrane protein OmpA and vibrio cholerae enterotoxin CTB fusion protein, a construction method and application, spore capsid protein CotY serves as an anchor point, coding genes of OmpA and CTB are recombined into a genome of bacillus subtilis 168 in a homologous double cross mode, and the recombinant bacillus subtilis is obtained. The recombinant bacillus subtilis can be stably inherited in the bacillus subtilis and displays edwardsiella tarda outer membrane protein OmpA and vibrio cholerae enterotoxin CTB on the spore surface; after fishes and mammals orally immunize the Edwardsiella tarda, the survival time and the survival rate of bodies infected with the Edwardsiella tarda are remarkably improved; the recombinant bacillus subtilis is used as an oral vaccine type micro-ecological immune preparation for preventing and treating Edwardsiella tarda infection of fishes and mammals, the immune procedure is remarkably simplified, the immune stress is reduced, the labor cost is saved, the effective action concentration in the intestinal tract is ensured, and the organism is induced to generate specific immune response.

The invention belongs to the technical field of biology, and discloses a construction method of a swine cholera attenuated salmonella recombinant strain capable of simultaneously expressing a haemophilus parasuis outer membrane protein GAPDH gene and an Omp26 gene, a plasmid connected with the haemophilus parasuis GAPDH gene and the Omp26 gene is adopted to transfect a swine cholera salmonella asd gene deleted strain, screening is carried out, and screening is carried out to obtain the swine cholera attenuated salmonella recombinant strain capable of simultaneously expressing the haemophilus parasuis outer membrane protein GAPDH gene and the Omp26 gene. The recombinant attenuated salmonella choleraesuis expressing the haemophilus parasuis GAPDH gene and the Omp26 gene is obtained. The strain can simultaneously express a haemophilus parasuis outer membrane protein GAPDH gene and an Omp26 gene, and compared with a strain which singly expresses Omp26, the strain has the advantage that the immune protection effect is obviously improved. Meanwhile, the invention also provides a construction method and application of the strain.

The invention belongs to the technical field of agricultural cultivation, and particularly relates to a method for inducing industrial cannabis sativa to generate female seeds through an inducer. The method comprises the following steps: (1) preparing an inducer mixed solution; the concentration of the nano-silver particles in the inducer mixed solution is 150 mg / L to 200 mg / L, the concentration of the gibberellin is 80 mg / L to 100 mg / L, and the concentration of the surfactant is 500 mg / L to 700 mg / L; (2) selecting industrial cannabis sativa which is about 10-15 days in the flowering period and is confirmed to be female plants, spraying the whole female plants of the industrial cannabis sativa in a mist form by using the inducer mixed solution in the near evening, avoiding stems, and preferably, spraying once every 2-3 days, spraying twice in total, and meanwhile, changing the illumination time, so that the female plants of the industrial cannabis sativa are obtained. The obtained industrial hemp seeds are all female. The method disclosed by the invention is high in all-female induction rate and good in stability.

The invention discloses a mutant of a rice GDSL lipase gene OsHGZ1 and application of the mutant in increasing the zinc content in grains. According to the present invention, the mutant with the mutation of the 195-site amino acid of the OsHGZ1 is obtained through EMS mutagenesis, and the verification results show that the zinc content of the mutant grain is twice the zinc content of the wild type; the OsHGZ1 is an excellent gene which can be applied to cultivation of high-zinc rice grains. The invention has very important significance for improving the nutritional quality of rice and improving the dietary health of human beings.

The invention discloses a saline-alkaline tolerance gene introduced rice high-yield breeding method. The method comprises the following steps: S1, gene screening and cloning: screening related genes with saline-alkaline tolerance; s2, vector construction: inserting the screened saline-alkaline tolerant gene into a proper expression vector; s3, transforming rice cells: introducing the vector carrying the saline-alkaline tolerant gene into the rice cells; s4, screening positive plants: confirming that the rice cells with the saline-alkaline tolerant genes are successfully transferred, and selecting the positive plants from the rice cells for next-step culture; s5, tissue culture and development: placing the positive plants on a sterile culture medium for tissue culture, and enabling the positive plants to develop into complete plants; s6, planting and harvesting: transplanting and planting the obtained transgenic rice plants, and harvesting the transgenic rice plants to obtain rice seeds. The saline-alkali soil-resistant gene disclosed by the invention is stronger in saline-alkali resistance, the yield is not influenced after the gene is planted in saline-alkali soil, and high-yield rice can be obtained.

The present invention discloses a rice Wx mutant protein, a mutant gene and its application, and also discloses a breeding method for creating quality-improved rice with transparent appearance and appropriately reduced amylose content by gene editing. The present invention uses gene editing technology for the first time to Wx By editing the gene with a single base, and through the screening of the offspring, new materials with T-DNA removed, transparent appearance, appropriately reduced amylose content, improved quality, and stable inheritance can be obtained in the T2 generation. Compared with breeding such as chemical mutagenesis and hybridization, gene editing directed improvement molecular breeding technology has the advantages of rapidity, accuracy, and efficiency. Combined with gene function marker genotype selection, or directly using other mature rice varieties as background materials for transformation and obtaining corresponding materials, it will greatly improve breeding efficiency and greatly accelerate the breeding process.

The invention provides a construction method of a non-small cell lung cancer histone H1.3 arginine methylation point mutation cell model. The construction method comprises the following steps: designing mutation primers H1.3 R80A-F and H1.3 R80A-R; the method comprises the following steps: by taking a pCDH-HA-H1.3-Flag plasmid as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting a mutation primer, digesting a product by DMT enzyme, and converting a competent cell to obtain a mutant plasmid; and co-transfecting the mutant plasmid and a helper plasmid to a packaging cell, collecting a virus solution, filtering, infecting an A549 cell, adding Polybrene to assist infection, culturing, and screening a stably transfected cell strain by using puromycin to obtain the recombinant plasmid. The invention also provides application of the mutant cell model obtained by the construction method. According to the invention, the blank of histone H1.3 methylation research is filled, the 80th arginine is clear as a core modification site, and the established model provides a new tool for lung cancer mechanism research and drug research and development.

The invention discloses a method for ultra-low temperature preservation and detoxification of pinellia ternata, comprising material culture, loading treatment, vitrification treatment, ultra-low temperature treatment, thawing and warming treatment, unloading treatment and regeneration plant culture. The pinellia ternata vitrification ultra-low temperature preservation technology system established by the invention can be applied to the preservation of pinellia ternata germplasm resources. Compared with field preservation and delayed growth preservation technology, the technology can greatly save manpower and material resources, is easy to operate, is not easily attacked by natural disasters, does not need to transfer plant materials regularly, occupies a small space, has low preservation cost, long preservation time, is genetically stable, and can preserve pinellia ternata germplasm for a long time; the technology can also be applied to the detoxification of pinellia ternata plants. After detoxification of test tube tubers of second-generation diseased plants of pinellia ternata, it was found through experimental verification that the detoxification rate of soybean mosaic virus (SMV) was 85.7%, and the detoxification rate of cucumber mosaic virus (CWV) was 57.1%, and the detoxification effect was better than that of commonly used heat treatment and stem tip detoxification.

The present invention provides an application of an apple yellow aphid Cmb4b gene and its dsRNA in preventing and controlling apple yellow aphids. The provided apple yellow aphid Cmb4b gene has an amino acid sequence of a protein encoded by it as SEQ ID NO: 2; and a nucleotide sequence as shown in SEQ ID NO: 1. The present invention also provides a dsRNA, which can reduce the expression level of the apple yellow aphid gene. In another aspect, the present invention also provides a biological product for preventing and controlling apple yellow aphids, wherein dsRNA and matrine are added, and the 72h mortality rate of the apple yellow aphid is 90%, and the biological control of the apple yellow aphid can be achieved efficiently. The present invention takes the apple yellow aphid as the research object, screens a target gene with a high lethality rate based on RNAi technology, obtains a dsRNA interference fragment, and provides a new target for the biological control of the apple yellow aphid.

The invention discloses a construction method and application of a systemic lupus erythematosus mouse model, the correlation between Rasgrp1 composite heterozygous mutation (p.T214I, p.K322X) and the phenotype of systemic lupus erythematosus of a mouse is found for the first time, and on the basis of the correlation, a novel systemic lupus erythematosus mouse model carrying the Rasgrp1 composite heterozygous mutation (p.T214I, p.K322X) is constructed for the first time. A foundation is laid for research on pathogenesis and intervention strategies of systemic lupus erythematosus, and the application prospect is wide.

The invention discloses a method for medium-short-term preservation and growth recovery of pinellia ternate. The method comprises the steps of material culture and disinfection, second-generation test-tube plantlet and second-generation tuber culture, medium-short-term preservation of third-generation tubers, growth recovery and propagation expansion. The Pinellia ternate medium-short term preservation technology can be applied to Pinellia ternate germplasm resource preservation, compared with field preservation, the Pinellia ternate medium-short term preservation technology can greatly save manpower and material resources, is easy and convenient to operate and not prone to being attacked by natural disasters, has the advantages of being small in occupied space, low in preservation cost, long in preservation time, stable in heredity and the like, and Pinellia ternate germplasm can be preserved in a medium-short term.

This application relates to a species of Pseudomonas aeruginosa. bpsR Genes, BpsR proteins and their applications, engineered bacteria, and drugs, among which bpsR The nucleotide sequence of the gene is shown in SEQ ID No. 1. This invention... bpsR Genes, through regulation in Pseudomonas aeruginosa bpsR Gene expression can regulate the virulence factors of Pseudomonas aeruginosa, such as biofilm production, pyocyanin production, motility, and elastase secretion levels. Furthermore, BpsR protein can be used as a new target for the development of drugs to prevent and treat Pseudomonas aeruginosa, which has important clinical significance for the prevention and control of Pseudomonas aeruginosa infection.

The invention provides a sheep embryo fibroblast line and application thereof, and belongs to the technical field of cell biology. The name of the sheep embryo fibroblast line is sheep embryo fibroblast OEF, the sheep embryo fibroblast line is preserved in China Center for Type Culture Collection, the address is Wuhan University, Wuhan, China, the preservation date is January 26, 2022, and the preservation number is CCTCC NO: C202212. The sheep embryo fibroblast line can be used for separation and culture of bovine and sheep pox viridae viruses such as LSDV, GTPV, SPPV and ORFV, has the characteristics of short proliferation time and high virus titer, and can avoid the risk that other viruses are polluted by viruses generated by primary cell separation.

The invention provides a skin organoid HPV infection model based on a human embryonic stem cell source and a construction method and application thereof, and relates to the technical field of molecular biology, the construction method comprises the following steps: integrating an HPV genome to an AAVS1 site of genome DNA of a human embryonic stem cell to obtain the human embryonic stem cell carrying the HPV genome, and performing induced differentiation and activation of the HPV genome to obtain the human embryonic stem cell carrying the HPV genome. A skin-like organ HPV infection model is formed, stable heredity and controllable expression of an HPV genome are achieved, virus particle assembly is achieved through induced differentiation, establishment of a virus replication environment, activation of HPV early gene expression, driving of HPV genome replication and induction of late gene expression, and the natural infection state of HPV is simulated. The technical problem that in the prior art, an infection model cannot simulate the life cycle of HPV dependent terminal differentiation is solved, and an irreplaceable technical platform is provided for research of an HPV pathogenic mechanism and research and development of antiviral drugs and vaccines.

The invention discloses a DNA structure for saccharomyces cerevisiae single-cell barcode labeling and chromatin state conjoint analysis and application thereof, and belongs to the technical field of molecular biology and single-cell multi-omics analysis. From the 5'end to the 3 'end, a fluorescent protein gene, a Tn5ME structure, a reverse transcription primer binding region, a CYC1 terminator fragment and a linearized shuttle plasmid skeleton are subjected to homologous recombination, and a pCM carrier is obtained; after NdeI / BamHI enzyme digestion, oligonucleotides with random sequences, which are subjected to annealing, amplification, Klenow filling-in and enzyme digestion, are connected, and transformation extraction is carried out, so that the DNA structure for saccharomyces cerevisiae single-cell barcode labeling and chromatin state combined analysis is obtained. On the basis of the constructed DNA structure, ATAC-seq and a bar code reverse transcription method are combined, important data related to the transcription state and chromatin state of a single cell can be obtained at the same time through bar code information, single cell pedigree tracking of the saccharomyces cerevisiae is achieved, and research on single cell epigenomics of the saccharomyces cerevisiae and other microorganisms is promoted.

The application discloses a deletion mutant of a rice LHCB5 gene and application thereof, and belongs to the field of gene editing. Through gene editing system, the 3'UTR region of the rice LHCB5 gene is precisely edited, and a 40bp homozygous deletion mutant of +16 to +55bp downstream of the termination codon TAA is obtained. The mutant can be stably inherited, and the growth phenotype is not significantly different from that of the wild type. Meanwhile, the resistance to rice blast and bacterial leaf blight is significantly improved, and the length of the disease spot is significantly reduced. The application first discloses the disease resistance regulation function of the 3'UTR of the LHCB5 gene, realizes the improvement of the disease resistance through the editing of the non-coding region, avoids the safety and agronomic trait risks brought by the transgene and the coding region editing, and the obtained mutant can be directly used for the breeding of the rice broad-spectrum disease resistance, and has important application value.

The present application relates to a high-yield Morchella MZ-12 and its cultivation method, belonging to the technical field of edible mushroom cultivation. The present application uses chemical reagent N-methyl-N'-nitro-N-nitrosoguanidine (NTG) mutagenesis method to first obtain a Morchella MZ-12, which is preserved in the China General Microbiological Culture Collection Center on March 23, 2023, with the preservation number of CGMCC No.40394; the strain has a shortest mushroom growth time shortened by 10 days compared with the original Morchella strain, has a higher yield, has a strong resistance to Streptomyces, has genetic stability, has a more thick and elastic flesh compared with the known Morchella varieties, has a more rich aroma, and has a better commodity nature.

This invention discloses a mutant attenuated strain of avian reovirus genotype II, wherein the σC protein amino acid sequence of the mutant strain includes mutations at positions 40, 45, 111, 180, 244, 273, and 300. Due to this mutation, compared to the parent wild-type strain, the pathogenicity to SPF chickens is significantly reduced, it does not cause persistent footpad swelling, and the viral load and shedding levels in tissues are extremely low, without affecting weight gain. Immunopotency evaluation shows that this mutant attenuated strain can induce high levels of specific antibodies, effectively controlling clinical symptoms, viral load in tissues, and shedding. This invention provides a safe and highly effective attenuated vaccine candidate strain for the prevention and control of avian reovirus genotype II, filling a corresponding technological gap.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PcWVD2 gene in enhancing the dwarfing ability of pear plants. The CDS (Coding Sequence) of the PcWVD2 gene is as shown in SEQ ID NO. 1. The amino acid sequence coded by the PcWVD2 gene is as shown in SEQ ID NO. 2. The invention reveals that the PcWVD2 gene has the function of regulating and controlling the dwarf character of the pear plant, the dwarf ability of the pear can be obviously enhanced by over-expressing the gene, the dwarf ability shows that the plant height is reduced, the internode is shortened and the root length is shortened, and an important candidate gene is provided for dwarf close planting cultivation of the pear tree. According to the method for enhancing the dwarfing ability of the pear plant, the endogenous dwarfing gene PcWVD2 is directly overexpressed by the pear plant through genetic transformation, the stably inherited dwarfing character can be realized, the dependence on dwarfing rootstock can be gotten rid of, and the cultivation management cost can be reduced.

This invention belongs to the field of trastuzumab technology, specifically relating to a Nod2 gene knockout CHO cell line, its preparation method, and its applications. The Nod2 gene in the knockout CHO cells is lost. The CHO cells stably express trastuzumab before Nod2 gene knockout. At the same cell density, the CHO cell line shows a 1.22-1.55-fold increase in trastuzumab expression. This invention knocks out the Nod2 gene in CHO cells using a CRISPR / Cas9 system, and also provides a method for constructing the cell line, a method for producing trastuzumab using the cell line, and applications of the cell line. The Nod2 gene knockout cell line significantly increases trastuzumab expression in suspension culture while effectively reducing the aggregation level of the target protein. Furthermore, the high-yield trastuzumab trait is stably inherited and does not significantly inhibit normal cell growth, providing a new stable platform for efficient trastuzumab production and possessing significant industrial application value.

The invention provides a DC2.4 cell with a GALECTIN-1 gene knocked out and application of the DC2.4 cell in antigen presentation research, and belongs to the technical field of gene engineering. The method comprises the following steps: constructing a recombinant CRISPR / Cas9 plasmid by using double-stranded DNA (Deoxyribonucleic Acid) as shown in SEQ ID No.3 and SEQ ID No.4, packaging the recombinant plasmid into a lentivirus, and infecting a cell by using the lentivirus, so as to obtain a cell in which the GALECTIN-1 gene is knocked out. A Western Blot detection result shows that the method realizes the knockout of the mouse GALECTIN-1 gene on the protein level, and the mouse GALECTIN-1 gene can be stably inherited. The sgRNA and the knockout vector disclosed by the invention can be used for directional knockout of the mouse GALECTIN-1 gene, have the characteristics of simplicity, convenience, high efficiency, rapidness, low cost and the like, and are of great significance in research on functions of the mouse GALECTIN-1 gene and antigen presentation of the mouse GALECTIN-1 gene in adaptive immunity.

The present invention specifically relates to an engineered bacterium of Serratia marcescens with stable genetic exogenous genes, BRC-CXG2-pCHSW, and its application, belonging to the field of microbial technology. The strain is classified and named as Serratia marcescens Serratia marcescens , deposited in the General Microbiology Center of China Microbiological Culture Collection Administration Committee, with the deposit number CGMCC NO.23125 and the deposit date August 6, 2021. The Serratia marcescens engineered bacteria can stably nptII-cry3AaT-hasA The exogenous gene can secrete and express Cry3AaT protein. The engineered Serratia marcescens bacteria can be used in the prevention and treatment of coleopteran pests and their offspring, and has high insecticidal activity.

The invention relates to a yellow flammulina velutipes C53 with a preservation number of GDMCC No.67334. The yellow flammulina velutipes C53 has the advantages of high yield, short growth cycle, more edible parts of stipes, difficulty in browning of stipe bases, long preservation time and stable inheritance, and is used for cultivation and breeding of flammulina velutipes. The invention also relates to an identification method of the flammulina velutipes C53, which comprises the following steps: by taking the DNA of the flammulina velutipes mycelium to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting the primer combination 1 and / or the primer combination 2, and detecting whether an amplification product has a band with a specific size so as to identify the C53. The screening method is simple to operate, and detection can be performed only by using mycelia; the detection time is only 2-3 hours, so that the breeding efficiency is improved, and the cost is greatly saved.

This invention discloses a high-glutathione-producing engineered Escherichia coli strain and its applications, belonging to the field of biotechnology; the engineered Escherichia coli strain is named ZMGSH01F, with accession number CCTCC M 20252236; the engineered Escherichia coli strain is based on Escherichia coli BL21(DE3) as the starting strain, with gene clusters knocked out. ptsH‐ ptsI‐crr And integrate at this site glf‐glk Fusion genes, knockout gene clusters gsiA‐gsiB‐gsiC Integrate the endogenous glutathione transporter gene at this site. cycDC Knockout of γ-glutamyltransferase gene ggt Simultaneously expressing the recombinant plasmid pRSFDuet- gshAB K177E‑E275K‑V513A - ppk2 The engineered Escherichia coli strain can efficiently produce glutathione through fermentation culture using glucose, monosodium glutamate, cysteine, and glycine as substrates.

The invention belongs to the field of biology, and relates to a construction method and application of a prefrontal cortex specific Gabre overexpression mouse model.The construction method comprises the steps that a CAG-LSL-mGabre-3-3xFlag-WPRE-PolyA gene segment is inserted into the H11 site of a mouse in a fixed point mode through the CRISPR / Cas9 technology; the sequence behind the H11 site of the recombined mouse is as shown in SEQ ID NO. 1. The CRISPR / Cas9 system and the homologous recombinant vector are microinjected into fertilized eggs of C57BL / 6JGpt mice, and F0-generation mice are obtained; the F0-generation positive mouse and the C57BL / 6JGpt mouse which are verified to be correct through PCR (Polymerase Chain Reaction) and sequencing comate to obtain an F1-generation positive mouse model capable of stably inheriting; and mating the F1-generation mouse with a Cntnap2-CreERT2 mouse to obtain an F2-generation mouse, and carrying out gene identification on the F2-generation mouse to finally obtain a stably inherited gene editing animal.