108 results about "Cell separation" patented technology

The invention provides a precise magnetic control whole blood rare cell flow magnetic separation system, a magnetic separation and identification method and application, and belongs to the technical field of micro-control flow biological detection. According to the invention, the magnetic separation chip can form a gradient magnetic field, and cells are fixed at a certain position on the sealing film under the combined action of magnetic field force and friction force, so that the separation of the cells is realized. Whole blood cell detection is carried out based on the gradient magnetic field, whole blood sample loading treatment can be directly carried out, the problem that the capture rate is reduced due to mechanical death, damage and stress gene expression caused by repeated elution / transfer steps is avoided, and the capture efficiency and cell activity of the chip are guaranteed; the magnetic field provided by electricity is not needed, the cost is low, the detection accuracy is high, the problems existing in existing CTC detection are solved, economic and practical values are achieved, further development of CTC detection can be promoted, and a novel and effective method is provided for complete blood cell separation detection.

The invention relates to a method for detecting activation of specific T cells after tuberculosis infection and application of the method. Specifically, the invention provides a reagent combination for detection, and the reagent combination comprises an anti-CD4 antibody (CD4 antibody), an anti-CD154 antibody (CD154 antibody), an IFN-gamma detection antibody and a tuberculosis specific antigen. According to the method, through peripheral whole blood collection, PBMC cell separation, T cell activation (activation), activation termination (blocking), antibody labeling, membrane rupture, fixation, intracellular cytokine dyeing, flow cytometry detection and result analysis and judgment, the ratio of the expression IFN-gamma cells in the CD4 + CD154 + cells can be rapidly and accurately determined, and by combining an ROC curve and a constructed judgment standard, the content of the IFN-gamma cells in the CD4 + CD154 + cells can be determined, and the content of the IFN-gamma cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells can be determined. And the sample to be detected is judged to be in a positive / negative / gray region, so that whether a patient is infected by the mycobacterium tuberculosis or not can be more accurately diagnosed, especially aiming at a whole blood sample from a special crowd.

The utility model discloses a differential adherent cell separation device which comprises a separation box body and an upper box cover, culture cavities and a one-way conducting assembly are arranged in the separation box body, the one-way conducting assembly is arranged between the culture cavities, the side face of the separation box body extends outwards to form a supporting part, the side edge of the supporting part protrudes upwards to form a baffle ring part, and the baffle ring part is connected with the upper box cover. A limiting slot is formed among the baffle ring part, the supporting part and the separating box body, and the four sides of the upper box cover extend downwards to form side ring parts. According to the utility model, the one-way conduction between the culture cavities is realized through the one-way conduction assembly, the communication state between the culture cavities can be controlled at a proper stage according to the actual condition of cell wall attachment, the cells are ensured to flow according to the expected direction and mode, and the accuracy and purity of cell separation are effectively improved; the upper box cover and the separation box body are tightly and stably connected, the upper box cover is prevented from loosening or displacing in the using process, and the overall sealing performance and stability of the device are guaranteed.

The present disclosure relates to a method of inhibiting nuclease activity in a biological sample. In accordance with certain embodiments of the present disclosure, the method comprises mixing a biological sample with a metal-chelating agent complex. The present disclosure also discloses a method of isolating a cell nucleus from a cell in a biological sample by using the metal-chelating agent complex, and a method of adding a deoxyribonucleotide to the 3'terminal of a deoxyribonucleic acid.

An apparatus and method for T-cell separation, activation, transduction and expansion. Three-dimensional (3D) bioreactors may be employed that include antibody coatings. Such 3D bioreactors can be employed for T-cell separation from peripheral blood mononuclear cells including attachment of T-cells to the 3D bioreactor surface for activation and transduction by lentivirus vectors to produce CAR T-cells. The CAR T-cells can then be expanded in a separate downstream bioreactor therein providing a scalable automated system.

The invention discloses a mouse primary cardiac endothelial cell extraction method, and belongs to the technical field of biological cell extraction. The method comprises the following steps: step 1, obtaining pure myocardial tissues; 2, the treated heart tissue is transferred into an intelligent tissue grinding system to be ground; step 3, transferring the ground heart tissue into a temperature-controlled enzymolysis reactor, automatically monitoring the digestion process, and terminating the reaction when a preset digestion degree is reached; step 4, carrying out primary cell separation, carrying out magnetic separation by using anti-CD31 magnetic beads, and specifically enriching heart endothelial cells; 5, transferring the purified endothelial cells to an intelligent microenvironment culture system, and automatically optimizing a culture medium replacement period to adapt to a cell metabolism state; and step 6, performing multi-dimensional quality evaluation on the endothelial cells, wherein the multi-dimensional quality evaluation comprises marker expression analysis, in-vitro lumen formation experiment, AI-assisted morphological analysis and digital quality scoring.

The utility model relates to the technical field of cell separation, and discloses a cell separation device, and an automatic cell vessel limiting mechanism comprises a connecting shaft rod, a connecting shaft core, a connecting shaft core and a cell vessel, the deflection axis is connected to the other end of the connecting shaft rod; one end of the motion shaft rod is connected to the deflection axis; the movable shaft center is connected to the other end of the movable shaft rod; and the clamping limiting frame is connected to the connecting shaft rod. The connecting shaft rod is pulled inwards through the deflection axis, the bottom end of the connecting shaft rod deflects around the connecting axis, the clamping limiting frame connected with the top end of the connecting shaft rod is driven to synchronously conduct clamping deflection, the clamping limiting frame clamps and fixes the cell vessel, the stability of the cell vessel is guaranteed, and meanwhile the cell vessel is prevented from being damaged. And the automatic cell vessel limiting mechanism can be used for fixedly clamping various cell vessels with different sizes.

The utility model discloses a primary cell extraction device, which relates to the technical field of cell extraction, and comprises a cell centrifugal machine and a collecting device, the bottom of the cell centrifugal machine is fixedly connected with the top of the collecting device, in the utility model, the device can automatically cut and crush a cell sample through the reverse rotation of a left group of cutters and a right group of cutters; manual intervention is greatly reduced, the cell extraction efficiency is improved, meanwhile, the crushing consistency of a packaged sample can be achieved, the purity and yield of subsequent cell extraction are improved, a cell centrifugal machine is started according to a preset separation program for primary cells, the centrifugal process is automatically completed, layering of the cells and impurities is achieved, and after centrifugation is finished, the cell extraction efficiency is improved. Separated cells and supernate can be accurately collected into corresponding containers through the collection device, tissue smashing, cell separation and collection are integrated in one device, the sample transfer frequency is reduced, the pollution risk is reduced, and the working efficiency is improved.

The invention relates to the technical field of cell culture, in particular to an efficient separation culture method for hepatocytes of small yellow croakers and a hepatocyte source identification method. The invention provides a method for efficiently separating and culturing hepatocytes of small yellow croakers, which comprises the following steps: selecting liver tissues of juvenile small yellow croakers, cleaning with a PBS (Phosphate Buffer Solution) containing high-concentration double antibodies, and digesting with pancreatin to quickly obtain hepatocytes; optimizing cell culture to obtain an L-15 culture medium containing 10% of FBS, 1% of sodium pyruvate and 0.2% of NaCl; and cell source identification is carried out by amplifying mitochondrial COI gene and 16S rRNA gene sequences. According to the method, the separation efficiency of the hepatocytes of the small yellow croakers is remarkably improved, the pollution rate is reduced, the result reliability is improved through double-gene molecular identification, the technical blank of construction of hepatocyte lines of the small yellow croakers is filled, and the method has wide application potential in the fields of aquaculture, fish physiology research, environmental toxicology detection and the like.

The present invention discloses a magnetically driven portable multi-cell separation device. According to one embodiment of the present invention, the magnetically driven portable multi-cell separation device includes: A microscope capable of nano-scale observation, A chip unit, positioned below the microscope, enabling selective cell sorting and capture, A magnetic field generator, which is adjacent to the chip unit and can control the magnetic field via a magnetic member, and A control unit, which controls the operation of the imaging unit, the chip unit, and the magnetic field generator.

The present invention relates to a method and platform for generating CAR-T cells and processing biological cells within a single functionally closed container. The method involves introducing host cells into a sealed container with multiple sterile channels, performing genetic modifications, and maintaining the cells under controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is obtained. The container also supports target cell separation using antibody or aptamer functionalized buoyant microbubbles, facilitating the separation of target cells from non-target cells during centrifugation. Waste separation and cell harvesting are performed using pneumatic pressure, enabling high purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, enabling high recovery and high purity of therapeutic cell populations. This integrated closed system approach ensures sterile conditions, efficient processing, and high quality cell products for advanced therapeutic applications.

The invention relates to collagenase as well as a coding gene and application thereof. The collagenase provided by the invention is a protein (a) or (b), wherein (a) is a protein composed of an amino acid sequence as shown in SEQ ID No: 1; and (b) a protein which is obtained by substituting, deleting or adding one or more amino acids in the amino acid sequence in (a), has collagen hydrolysis or fat dissolving activity and is derived from (a). The collagenase provided by the invention can be applied to cell separation and tissue digestion, fat dissolution or collagen dissolution, and can also be applied to preparation of medicines for treating Doltt's contracture, Petroleum disease and lumbar disc herniation and degreasing in the field of beauty.

The utility model discloses a cell separation and detection device. The cell separation and detection device comprises a machine body and a conical circular plate, by adopting the limiting disc and the adjusting disc, when test tubes loaded with cells are placed, a person can lift the limiting disc according to different sizes of the test tubes, so that the adjusting shaft can rotate, and then the adjusting disc is driven to rotate through the rotation of the adjusting shaft; at the moment, limiting grooves with corresponding sizes in the surfaces of the adjusting discs can move to the openings, then after the test tubes are inserted, limiting blocks can be inserted into the openings and the positioning grooves again through the limiting discs under the pushing of extrusion springs, and the limiting discs and the test tubes are fixed; the test tube can be effectively prevented from falling off during high-speed centrifugation by being matched with clamping and limiting of the upper limiting groove, meanwhile, the test tube can be operated only by lifting and rotating, the operation is very simple and labor-saving, the test tube can be better used conveniently, and the test tube has the advantages of being convenient to fix, good in limiting effect and labor-saving in operation.

A method for enriching glucagon producing alpha cells and / or insulin producing beta cells in a mixture of cells, comprising labelling the cell surface marker CD99R in a starting mixture of cells with a label and separating the cells that are positive for the cell surface marker from cells that are negative for the cell surface marker to obtain a final mixture of cells that is more enriched in alpha cells and / or beta cells compared to the starting mixture. A use of an antibody specific for CD99R for detecting glucagon producing alpha cells and / or insulin producing beta cells.

An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and / or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

The application belongs to the technical field of pathological grading, and particularly relates to a lymphoma histological grading method, which comprises the following steps: screening a plurality of regions of interest rich in diagnostic information in a lymphoma pathological section image; inputting the regions of interest into a trained Hover-Net nuclear segmentation model to output a cell separation image; the cell separation image can mark the regions of different cell types, the positions of each cell and the classification information thereof; inputting the cell separation image into a classifier to output a histological grading result and the number of each cell type. The application improves the diagnostic accuracy and efficiency of follicular lymphoma pathological grading by efficiently recognizing lymphoma pathological section images of different pathological grades through a Hover-Net nuclear segmentation model.

The invention discloses a preparation method of crosslinked polystyrene porous microspheres with controllable surface aperture and particle size, which comprises the following steps: dispersing monodisperse polystyrene microspheres with different particle sizes and molecular weights prepared by emulsion polymerization, soap-free emulsion polymerization or dispersion polymerization in a lauryl sodium sulfate aqueous solution; and activating the seed microspheres by using a micromolecular swelling agent, then adding a monomer dissolved with an initiator and a cross-linking agent for second-step swelling, and after swelling is finished, carrying out heating polymerization reaction to prepare the cross-linked polystyrene porous microspheres. According to the preparation method, nano-scale PS seed balls with different molecular weights are prepared firstly, and then the cross-linked PS porous microspheres prepared through swelling polymerization are smaller in particle size (lt; 2m), so that the particle size distribution range of the cross-linked PS porous microspheres is remarkably reduced. The prepared porous microspheres are small and adjustable in particle size (800 nm <-2 > m), controllable in pore diameter and good in suspension property, can be kept not to settle for a long time, and have more advantages in practical application of chromatographic packing, carrier catalysis, targeted drugs, cell separation, immunoassay and the like.

The invention provides an autoimmune disease physical therapeutic apparatus based on a microfluidic technology and dendritic cells, and belongs to the technical field of biomedical engineering. The therapeutic apparatus provided by the invention comprises an automatic blood sampling system, a target cell separation system, a micro-fluidic chip system, a high-precision liquid flow driving system, a real-time monitoring system, a cell feedback system and an intelligent control system which are connected in sequence. The therapeutic apparatus can integrally complete the whole process from patient blood sampling, target cell separation, physical regulation and control to cell reinfusion, and solves the problem that a safe, effective and controllable autoimmune disease physical therapeutic apparatus is lacked in the prior art.

1. The name of the design product: cyclone centrifuge. 2. The use of the design product: the design product is used for reagent, solvent, chemical, cell separation, microbial sample separation and treatment. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

Methods (300), devices, and systems of processing blood are described. The method (300) comprises the steps of: obtaining (312) blood from a patient coupled to a single blood processing device to form a closed loop between the patient and the blood processing device; collecting (314) bulk mononuclear blood cells from the blood by leukapheresis implemented using the blood processing device in the closed loop; and enriching (316) concurrently target cells separated from non-target cells in the bulk mononuclear blood cells using the blood processing device in the closed loop.

The invention aims to provide a method for separating and amplifying placenta decidua matrix cells in a perinatal period. The method comprises the following steps: adding decidua tissues into a culture medium A containing collagenase for digestion treatment; cleaning to obtain a cell suspension A, and sieving to obtain a cell suspension B; mixing the cell suspension B with a Ficoll solution, and carrying out primary gradient centrifugation; mixing the cell layer and the supernate layer with normal saline, and then carrying out secondary gradient centrifugation; and culturing the cell block mass and the culture medium B in an incubator. According to the method, the success rate of separation and amplification of the placenta decidua interstitial cells in the perinatal period can be increased, the separated and proliferated cells have group cell biomarker expression for tissue repair, and the separation technical method is improved, so that highly purified cells are obtained.

The invention relates to the technical field of immune cells, in particular to a preparation method of human placenta-derived NK cells. The preparation method comprises the following steps: S1, separating placenta tissues; s2, placenta tissue digestion; S3, mononuclear cell separation; S4, NK cell sorting; and 5, NK cell multiplication culture.

The utility model discloses a sterile needle cylinder type spleen lymphocyte separation and extraction device, including separation part, grinding part, collection part, and separation part is needle cylinder shape, including detachable connection's upper cylinder, lower cylinder, and the lower end detachable connection cell screen of upper cylinder, and the lower end detachable connection collection part of lower cylinder, and grinding part includes push rod, rocker, and the lower end of push rod is equipped with piston head, and piston head and upper cylinder closely slide and cooperate, and the center of push rod and piston head is equipped with the through -hole of continuity, and the rocker is slid and is arranged in the through -hole, and the upper end is stretched out push rod outside and is connected the rocking handle, and the lower end is stretched out piston head outside and is connected the grinding pestle, and the lateral wall of upper cylinder is equipped with liquid adding pipe, and one end of liquid adding pipe is located in the upper cylinder, and the other end is located in the upper cylinder outside. The utility model can carry out the coherent operation of organization grinding, cell release, impurity filtration, lymphocyte enrichment under the semi -closed environment, reduces the sample pollution risk, and promotes the repeatability of experiment.

The application discloses a cell separation device, which comprises a centrifugal chamber and a rotating driving part for driving the rotation of the centrifugal chamber, a joint assembly is arranged on the centrifugal chamber, a liquid inlet interface, a waste outlet interface and a suction interface are connected to the joint assembly, a flow guide seat is arranged in the centrifugal chamber, a liquid inlet channel, a waste outlet channel and a suction channel are arranged on the flow guide seat, the liquid inlet interface is communicated with the liquid inlet channel through a first connecting pipe, the waste outlet interface is communicated with the waste outlet channel through a second connecting pipe, the suction interface is communicated with the suction channel through a third connecting pipe, and the suction channel extends to the end of the centrifugal chamber. A continuous separation method comprises the following steps: a) filling a buffer solution; b) filling a liquid containing target cells; c) cell layering; d) cell separation; or d') target cell washing. The application has the advantages of simple structure, compactness, continuous centrifugal separation, and the ability to process a larger volume of liquid.

The utility model relates to the technical field of tumor cell separation and collection, in particular to a tumor cell separation and collection device which comprises a table body, a supporting assembly, a circulating assembly, a flowing assembly, a separation bin, a separation motor, a separation shaft, a first gear, a second gear, a catching rod and a filtering membrane. A supporting assembly playing a supporting role is arranged at the lower end of the table body, a circulating assembly playing a circulating role is arranged on the inner side of the table body, and a flowing assembly playing a flowing role is arranged on one side of the table body. The tumor cells are rapidly and efficiently separated and collected in a centrifugal rotation, filter membrane filtration and fixed capture mode, so that the time spent on collecting the tumor cells by the device is shortened, the tumor cell separation and collection effect of the device is improved, medical workers can obtain data more rapidly and make judgment, and the efficiency of collecting the tumor cells is improved. And misjudgment caused by data deviation due to poor tumor cell separation effect of the device is reduced.

The invention discloses a phage multifunctional affinity interface as well as a preparation method and application thereof, and belongs to the field of functional biological materials. The preparation method of the phage multifunctional affinity interface comprises the following steps: modifying a phenylboronic acid group by using pVIII protein on the side wall of an M13 phage as a bracket; connecting histidine on the protein of the M13 bacteriophage pIII to the nickel magnetic microbead; the method comprises the following steps: carrying out fluorescence labeling on a biotinylated aptamer targeting EpCAM positive cells, hybridizing with a complementary chain of a labeled fluorescence quencher, and coupling with avidin according to a molar ratio of 4: 1 to obtain an avidin-biotinylated DNA switch; and then the M13 bacteriophage-nickel magnetic microbead containing the phenylboronic acid group is coupled with an avidin-biotinylated DNA (Deoxyribose Nucleic Acid) switch. The capture efficiency of the multifunctional affinity interface to MCF-7 cells reaches 94.2%, the release efficiency reaches 92.7%, and the multifunctional affinity interface has reproducibility and can be used for multiple rounds of cell separation.

The present application belongs to the technical field of tumor detection and cell separation, and particularly relates to a kit for separating and recovering after immunocapture of circulating tumor cells, a preparation method and application. The kit comprises an immunocapture module, a dissociation and recovery module, a cell protection module and elution and supplement factor solutions. The immunocapture module adopts magnetic microspheres modified by double antibody fusion proteins, can recognize multiple markers such as EpCAM and Vimentin, and realizes efficient capture of heterogeneous CTCs. The dissociation and recovery module uses a low-temperature buffer system and a cleavable cross-linking agent to release cells gently. The cell protection module maintains the integrity and activity of the cell membrane during the separation process. The method of the present application can ensure the capture efficiency while obtaining high-activity CTCs, and is suitable for single-cell sequencing, drug sensitivity analysis, organoid culture and patient-derived cell line construction and other preclinical research.

The present disclosure describes a sperm separation device (1). The device comprises a sample zone (5) configured to receive semen, a collection zone (15) fluidly connected to the sample zone by a migration zone (10). A cover (20) is disposed over portions of the migration region and the collection region, where the migration region comprises a groove for guiding sperm in the seminal fluid, and where sperm in the seminal fluid migrates to the collection region via the groove.

The invention relates to the technical field of cell separation and purification and cell biological detection, in particular to a method for separating and purifying fetal NRBC from peripheral blood of a pregnant woman. The invention provides a method for separating and purifying fetal NRBC from peripheral blood of a pregnant woman, which is characterized in that a LukSF and LukAB bi-component perforation toxin is designed and utilized to specifically recognize and split a CXCR1 / 2 chemotactic factor receptor and a CD11b / CD18 integrin receptor on the surface of maternal leukocyte, and the fetal NRBC is completely reserved due to lack of corresponding receptors, so that the fetal NRBC can be separated and purified. Therefore, leukocyte pollution in nucleated red blood cells of a peripheral blood fetus of a pregnant woman is efficiently removed. According to the method, the purity of the extracted fetal NRBC can be improved, the false positive of gene detection is greatly reduced, the activity and integrity of the NRBC are reserved, and the accurate diagnosis requirements of chromosome karyotype analysis, monogenic disease detection and the like are met; the method is simple to operate and low in cost, can be directly adapted to existing clinical laboratory equipment, and has large-scale popularization value.

This invention discloses a method for obtaining high-purity NK cells using apheresis, belonging to the field of cell culture. The method includes the following steps: (1) obtaining peripheral blood mononuclear cells using the apheresis blood component method; (2) adding NK cell sorting reagent to the peripheral blood mononuclear cells, mixing evenly, and allowing to stand at room temperature to obtain a cell mixture; then adding the cell mixture to the upper layer of lymphocyte separation medium, centrifuging, removing the white membrane layer and above the transparent plasma, washing the harvested cell fluid to obtain enriched NK cells; (3) purifying using CD56 positively selected magnetic beads to obtain purified NK cells; (4) inoculating the purified NK cells into a culture container coated with CD16 antibody and allowing to stand. This method can obtain NK cells with a purity of over 99%, and the cells can expand hundreds of times after 17 days of in vitro culture, exhibiting strong anti-tumor function at low target ratios.