146 results about "Cell separation" patented technology

The invention relates to the technical field of cell separation and culture, in particular to a method for separating and culturing haematochrome cells of plectropomus leopardus. The separation method of the plectropomus leopardus haematochrome cells is created for the first time, the EDTA digestive juice is used for pre-digesting epidermis cells on the outer layers of the plectropomus leopardus fins, then the collagenase digestive juice is used for digesting connective tissues (pigmented cells) of internal dermis, digestive enzymes such as trypsin and other mechanical methods which have great damage to the cells are not used, and the method is suitable for large-scale production of the plectropomus leopardus haematochrome cells. The plectropomus leopardus haematochrome cell can reduce cell damage, can be effectively separated to obtain the plectropomus leopardus haematochrome cell which is high in purity, good in activity and capable of being cultured in vitro, and lays a foundation for subsequent experiments such as single cell sequencing, cell in-vitro culture and molecular function verification.

The invention provides a precise magnetic control whole blood rare cell flow magnetic separation system, a magnetic separation and identification method and application, and belongs to the technical field of micro-control flow biological detection. According to the invention, the magnetic separation chip can form a gradient magnetic field, and cells are fixed at a certain position on the sealing film under the combined action of magnetic field force and friction force, so that the separation of the cells is realized. Whole blood cell detection is carried out based on the gradient magnetic field, whole blood sample loading treatment can be directly carried out, the problem that the capture rate is reduced due to mechanical death, damage and stress gene expression caused by repeated elution / transfer steps is avoided, and the capture efficiency and cell activity of the chip are guaranteed; the magnetic field provided by electricity is not needed, the cost is low, the detection accuracy is high, the problems existing in existing CTC detection are solved, economic and practical values are achieved, further development of CTC detection can be promoted, and a novel and effective method is provided for complete blood cell separation detection.

The invention provides a multi-channel viscoelastic micro-fluidic chip, a manufacturing method and a method for separating MCF-7 cells. The multi-channel viscoelastic micro-fluidic chip comprises a first multi-stage shunting module, a pipette, a second multi-stage shunting module, a single-stage viscoelastic micro-fluidic sub-module and a third multi-stage shunting module, the first multi-stage shunting module is connected with one inlet of the single-stage viscoelastic micro-fluidic sub-module through a pipette; the second multi-stage shunting module is connected with the other inlet of the single-stage viscoelastic micro-fluidic sub-module; and the outlet of the single-stage viscoelastic micro-fluidic sub-module is connected with the third multi-stage shunting module. According to the method, the PEO solution is used as sheath fluid, and the action of elastic force is introduced to cells, so that CTC can be directly separated from undiluted human blood in an ultrahigh-flux manner, and the limitation that erythrocytes need to be split and a blood sample needs to be diluted by dozens of times before cell separation in traditional inertial microfluidics is broken through; and the complexity of operation and the risk of sample pollution are reduced.

The invention relates to the field of biotechnology and biological materials, in particular to a vesicle chip and application thereof. The vesicle is characterized in that a macromolecule hydrogel solution forms vesicle liquid drops with a certain diameter through a microchannel under shearing of gas, the vesicle liquid drops are atomized and crosslinked to form the solidified vesicle, and the diameter of the vesicle ranges from 50 micrometers to 800 micrometers; the vesicles are vesicles containing cells. The vesicles of different sizes and the highly-bionic vesicle fusion chip can be used for preparing homogenized tumor organs, the extracellular matrix environment in the body and the interaction among multiple cells can be effectively simulated, the reversible hydrogel curing technology facilitates cell separation and detection, and the application prospect is wide. Meanwhile, the processes of tumor metastasis, blood vessel invasion and the like can be simulated.

The invention relates to a method for detecting activation of specific T cells after tuberculosis infection and application of the method. Specifically, the invention provides a reagent combination for detection, and the reagent combination comprises an anti-CD4 antibody (CD4 antibody), an anti-CD154 antibody (CD154 antibody), an IFN-gamma detection antibody and a tuberculosis specific antigen. According to the method, through peripheral whole blood collection, PBMC cell separation, T cell activation (activation), activation termination (blocking), antibody labeling, membrane rupture, fixation, intracellular cytokine dyeing, flow cytometry detection and result analysis and judgment, the ratio of the expression IFN-gamma cells in the CD4 + CD154 + cells can be rapidly and accurately determined, and by combining an ROC curve and a constructed judgment standard, the content of the IFN-gamma cells in the CD4 + CD154 + cells can be determined, and the content of the IFN-gamma cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells in the CD4 + CD154 + cells can be determined. And the sample to be detected is judged to be in a positive / negative / gray region, so that whether a patient is infected by the mycobacterium tuberculosis or not can be more accurately diagnosed, especially aiming at a whole blood sample from a special crowd.

The utility model discloses a differential adherent cell separation device which comprises a separation box body and an upper box cover, culture cavities and a one-way conducting assembly are arranged in the separation box body, the one-way conducting assembly is arranged between the culture cavities, the side face of the separation box body extends outwards to form a supporting part, the side edge of the supporting part protrudes upwards to form a baffle ring part, and the baffle ring part is connected with the upper box cover. A limiting slot is formed among the baffle ring part, the supporting part and the separating box body, and the four sides of the upper box cover extend downwards to form side ring parts. According to the utility model, the one-way conduction between the culture cavities is realized through the one-way conduction assembly, the communication state between the culture cavities can be controlled at a proper stage according to the actual condition of cell wall attachment, the cells are ensured to flow according to the expected direction and mode, and the accuracy and purity of cell separation are effectively improved; the upper box cover and the separation box body are tightly and stably connected, the upper box cover is prevented from loosening or displacing in the using process, and the overall sealing performance and stability of the device are guaranteed.

The present disclosure relates to a method of inhibiting nuclease activity in a biological sample. In accordance with certain embodiments of the present disclosure, the method comprises mixing a biological sample with a metal-chelating agent complex. The present disclosure also discloses a method of isolating a cell nucleus from a cell in a biological sample by using the metal-chelating agent complex, and a method of adding a deoxyribonucleotide to the 3'terminal of a deoxyribonucleic acid.

An apparatus and method for T-cell separation, activation, transduction and expansion. Three-dimensional (3D) bioreactors may be employed that include antibody coatings. Such 3D bioreactors can be employed for T-cell separation from peripheral blood mononuclear cells including attachment of T-cells to the 3D bioreactor surface for activation and transduction by lentivirus vectors to produce CAR T-cells. The CAR T-cells can then be expanded in a separate downstream bioreactor therein providing a scalable automated system.

The invention discloses a mouse primary cardiac endothelial cell extraction method, and belongs to the technical field of biological cell extraction. The method comprises the following steps: step 1, obtaining pure myocardial tissues; 2, the treated heart tissue is transferred into an intelligent tissue grinding system to be ground; step 3, transferring the ground heart tissue into a temperature-controlled enzymolysis reactor, automatically monitoring the digestion process, and terminating the reaction when a preset digestion degree is reached; step 4, carrying out primary cell separation, carrying out magnetic separation by using anti-CD31 magnetic beads, and specifically enriching heart endothelial cells; 5, transferring the purified endothelial cells to an intelligent microenvironment culture system, and automatically optimizing a culture medium replacement period to adapt to a cell metabolism state; and step 6, performing multi-dimensional quality evaluation on the endothelial cells, wherein the multi-dimensional quality evaluation comprises marker expression analysis, in-vitro lumen formation experiment, AI-assisted morphological analysis and digital quality scoring.

The utility model relates to the technical field of cell separation, and discloses a cell separation device, and an automatic cell vessel limiting mechanism comprises a connecting shaft rod, a connecting shaft core, a connecting shaft core and a cell vessel, the deflection axis is connected to the other end of the connecting shaft rod; one end of the motion shaft rod is connected to the deflection axis; the movable shaft center is connected to the other end of the movable shaft rod; and the clamping limiting frame is connected to the connecting shaft rod. The connecting shaft rod is pulled inwards through the deflection axis, the bottom end of the connecting shaft rod deflects around the connecting axis, the clamping limiting frame connected with the top end of the connecting shaft rod is driven to synchronously conduct clamping deflection, the clamping limiting frame clamps and fixes the cell vessel, the stability of the cell vessel is guaranteed, and meanwhile the cell vessel is prevented from being damaged. And the automatic cell vessel limiting mechanism can be used for fixedly clamping various cell vessels with different sizes.

The invention relates to the technical field of cell separation and extraction, and provides a method for extracting extracellular vesicles from induced sputum. Comprising the following steps: S1, atomizing and collecting induced sputum, picking out sputum plugs, and adding an isotonic phosphate buffer solution which contains 0.1 wt% of drocisteine and 0.1 wt% of tween-20 and has the pH value of 6.0 for dissolving, so as to obtain an induced sputum dissolving solution rich in extracellular vesicles; wherein the dissolving temperature is 37 + / -0.1 DEG C, and digestion is performed for 15 + / -1 min under the condition that the rotating speed of a shaking table is 50 + / -1 rpm; and S2, extracting the extracellular vesicles from the induced sputum dissolving solution through a negative pressure size exclusion chromatography method. According to the invention, the multi-component dissolving agent of the hydroxymethylsteine and the Tween-20 is adopted to promote the dissolving of sputum and protect protein and nucleic acid in extracellular vesicles from being degraded; the negative pressure size exclusion chromatography can accurately control the flow velocity driven by negative pressure, ensures stable migration of extracellular vesicles in a chromatographic column, and avoids vesicle rupture caused by too fast flow velocity or low separation efficiency caused by too slow flow velocity.

The utility model discloses a primary cell extraction device, which relates to the technical field of cell extraction, and comprises a cell centrifugal machine and a collecting device, the bottom of the cell centrifugal machine is fixedly connected with the top of the collecting device, in the utility model, the device can automatically cut and crush a cell sample through the reverse rotation of a left group of cutters and a right group of cutters; manual intervention is greatly reduced, the cell extraction efficiency is improved, meanwhile, the crushing consistency of a packaged sample can be achieved, the purity and yield of subsequent cell extraction are improved, a cell centrifugal machine is started according to a preset separation program for primary cells, the centrifugal process is automatically completed, layering of the cells and impurities is achieved, and after centrifugation is finished, the cell extraction efficiency is improved. Separated cells and supernate can be accurately collected into corresponding containers through the collection device, tissue smashing, cell separation and collection are integrated in one device, the sample transfer frequency is reduced, the pollution risk is reduced, and the working efficiency is improved.

The invention relates to the technical field of cell culture, in particular to an efficient separation culture method for hepatocytes of small yellow croakers and a hepatocyte source identification method. The invention provides a method for efficiently separating and culturing hepatocytes of small yellow croakers, which comprises the following steps: selecting liver tissues of juvenile small yellow croakers, cleaning with a PBS (Phosphate Buffer Solution) containing high-concentration double antibodies, and digesting with pancreatin to quickly obtain hepatocytes; optimizing cell culture to obtain an L-15 culture medium containing 10% of FBS, 1% of sodium pyruvate and 0.2% of NaCl; and cell source identification is carried out by amplifying mitochondrial COI gene and 16S rRNA gene sequences. According to the method, the separation efficiency of the hepatocytes of the small yellow croakers is remarkably improved, the pollution rate is reduced, the result reliability is improved through double-gene molecular identification, the technical blank of construction of hepatocyte lines of the small yellow croakers is filled, and the method has wide application potential in the fields of aquaculture, fish physiology research, environmental toxicology detection and the like.

The present invention discloses a magnetically driven portable multi-cell separation device. According to one embodiment of the present invention, the magnetically driven portable multi-cell separation device includes: A microscope capable of nano-scale observation, A chip unit, positioned below the microscope, enabling selective cell sorting and capture, A magnetic field generator, which is adjacent to the chip unit and can control the magnetic field via a magnetic member, and A control unit, which controls the operation of the imaging unit, the chip unit, and the magnetic field generator.

The invention relates to the technical field of cell filtration, and particularly discloses a cell separation filter which comprises a mounting frame, a filter body is fixedly mounted in the mounting frame, an inner cavity is formed in the filter body, a cell sieve is slidably mounted on the side wall of the inner cavity, a drainage device is fixedly mounted on the side wall of the inner cavity below the cell sieve, and the cell separation filter further comprises a turnover assembly. The liquid dropping assembly and the overturning assembly are matched to perform double-sided infiltration on the cell sieve, so that the cell sieve with the two sides fully infiltrated can reduce friction between a cell suspension and a sieve mesh and prevent cell clusters or tissue blocks from blocking the sieve mesh, thereby improving the filtering efficiency, being beneficial to protecting cells and improving the cell quality. According to the utility model, cell damage caused by drying or friction in the filtering process is reduced, cell activity and integrity are maintained, and the wetted screen enables cell suspension to pass through the screen holes more easily and uniformly, so that local over-fast flow or blockage is avoided, and the filtering uniformity and effect are improved.

The present invention relates to a method and platform for generating CAR-T cells and processing biological cells within a single functionally closed container. The method involves introducing host cells into a sealed container with multiple sterile channels, performing genetic modifications, and maintaining the cells under controlled conditions monitored by real-time feedback until a desired fraction of genetically modified cells is obtained. The container also supports target cell separation using antibody or aptamer functionalized buoyant microbubbles, facilitating the separation of target cells from non-target cells during centrifugation. Waste separation and cell harvesting are performed using pneumatic pressure, enabling high purity cell recovery. The platform includes modules for cell enrichment, sequestration, and isolation, enabling high recovery and high purity of therapeutic cell populations. This integrated closed system approach ensures sterile conditions, efficient processing, and high quality cell products for advanced therapeutic applications.

The invention relates to collagenase as well as a coding gene and application thereof. The collagenase provided by the invention is a protein (a) or (b), wherein (a) is a protein composed of an amino acid sequence as shown in SEQ ID No: 1; and (b) a protein which is obtained by substituting, deleting or adding one or more amino acids in the amino acid sequence in (a), has collagen hydrolysis or fat dissolving activity and is derived from (a). The collagenase provided by the invention can be applied to cell separation and tissue digestion, fat dissolution or collagen dissolution, and can also be applied to preparation of medicines for treating Doltt's contracture, Petroleum disease and lumbar disc herniation and degreasing in the field of beauty.

The invention belongs to the technical field of cell smears, and discloses a cell filtering, sorting and enriching smear device which comprises an accommodating and resuspending mechanism, a filtering mechanism and a smear mechanism. The accommodating and resuspending mechanism comprises a sample accommodating assembly and a resuspending assembly, the sample accommodating assembly and the resuspending assembly can be coaxially and integrally connected or mutually independent, the sample accommodating assembly accommodates a liquid biological sample, and the resuspending assembly accommodates resuspending liquid; the filtering mechanism is detachably connected to the sample containing assembly and further detachably connected to the resuspension assembly, a filtering membrane is arranged in the filtering mechanism, and a liquid biological sample is filtered and enriched through the filtering membrane and then is resuspended to obtain a cell suspension; the smear mechanism is connected to the centrifugal smear machine and can bear a glass slide and contain the filtering mechanism, and the cell suspension can flow to the glass slide from the filtering mechanism. The cell filtering, sorting, enriching and smearing device can realize cell separation, enrichment and smearing processes, prevent cell loss, improve the smearing quality and shorten the smearing time.

The utility model discloses a cell separation and detection device. The cell separation and detection device comprises a machine body and a conical circular plate, by adopting the limiting disc and the adjusting disc, when test tubes loaded with cells are placed, a person can lift the limiting disc according to different sizes of the test tubes, so that the adjusting shaft can rotate, and then the adjusting disc is driven to rotate through the rotation of the adjusting shaft; at the moment, limiting grooves with corresponding sizes in the surfaces of the adjusting discs can move to the openings, then after the test tubes are inserted, limiting blocks can be inserted into the openings and the positioning grooves again through the limiting discs under the pushing of extrusion springs, and the limiting discs and the test tubes are fixed; the test tube can be effectively prevented from falling off during high-speed centrifugation by being matched with clamping and limiting of the upper limiting groove, meanwhile, the test tube can be operated only by lifting and rotating, the operation is very simple and labor-saving, the test tube can be better used conveniently, and the test tube has the advantages of being convenient to fix, good in limiting effect and labor-saving in operation.

A method for enriching glucagon producing alpha cells and / or insulin producing beta cells in a mixture of cells, comprising labelling the cell surface marker CD99R in a starting mixture of cells with a label and separating the cells that are positive for the cell surface marker from cells that are negative for the cell surface marker to obtain a final mixture of cells that is more enriched in alpha cells and / or beta cells compared to the starting mixture. A use of an antibody specific for CD99R for detecting glucagon producing alpha cells and / or insulin producing beta cells.

An exemplary method and system is disclosed that facilitate the integration of multiplexed single-cell impedance cytometry in a high throughput format, which can be deployed upstream from microfluidic sample preparation and / or downstream to microfluidic cell separation. In exemplary method and system may employ impedance-based quantification of cell electrophysiology on the same microfluidic chip (i.e., “on-chip”) to provide distinguishing phenotypic information on the sample, without the need for additional sample handling, preparation or dilution steps as would be needed for other flow cytometry techniques.

The application belongs to the technical field of pathological grading, and particularly relates to a lymphoma histological grading method, which comprises the following steps: screening a plurality of regions of interest rich in diagnostic information in a lymphoma pathological section image; inputting the regions of interest into a trained Hover-Net nuclear segmentation model to output a cell separation image; the cell separation image can mark the regions of different cell types, the positions of each cell and the classification information thereof; inputting the cell separation image into a classifier to output a histological grading result and the number of each cell type. The application improves the diagnostic accuracy and efficiency of follicular lymphoma pathological grading by efficiently recognizing lymphoma pathological section images of different pathological grades through a Hover-Net nuclear segmentation model.

The invention discloses a preparation method of crosslinked polystyrene porous microspheres with controllable surface aperture and particle size, which comprises the following steps: dispersing monodisperse polystyrene microspheres with different particle sizes and molecular weights prepared by emulsion polymerization, soap-free emulsion polymerization or dispersion polymerization in a lauryl sodium sulfate aqueous solution; and activating the seed microspheres by using a micromolecular swelling agent, then adding a monomer dissolved with an initiator and a cross-linking agent for second-step swelling, and after swelling is finished, carrying out heating polymerization reaction to prepare the cross-linked polystyrene porous microspheres. According to the preparation method, nano-scale PS seed balls with different molecular weights are prepared firstly, and then the cross-linked PS porous microspheres prepared through swelling polymerization are smaller in particle size (lt; 2m), so that the particle size distribution range of the cross-linked PS porous microspheres is remarkably reduced. The prepared porous microspheres are small and adjustable in particle size (800 nm <-2 > m), controllable in pore diameter and good in suspension property, can be kept not to settle for a long time, and have more advantages in practical application of chromatographic packing, carrier catalysis, targeted drugs, cell separation, immunoassay and the like.

The invention provides a single-cell suspension for increasing the ratio of pancreatic acellular cells and a preparation method of the single-cell suspension. The preparation method comprises the following steps: taking materials; a collagenase digestion step: performing mixed digestion on the small pancreas blocks and a collagenase dissociation liquid, and collecting acinus cells through gradient centrifugal separation; and a single cell separation step: dispersing the alveolar cells into single cells by using the recombinant trypsin substitute. The collagen enzyme dissociation liquid in a specific combination is adopted, cold digestion is not needed, and digestion can be completed within 30 minutes; a gradient centrifugation mode is adopted to separate the acinus cells, so that the purity of target cells is improved; when the recombinant trypsin substitute is used for single cell separation, single cells can be rapidly and efficiently obtained, and repeated filtration is not needed. Besides, the cell viability of the mouse pancreas single-cell suspension obtained by the method is higher than 90%, the proportion of acinus cells reaches 70% or above, and a high-quality cell sample is provided for subsequent experimental research.

The utility model discloses a screening device for cell separation. The screening device comprises a shell and a wicking assembly arranged above the shell, an automatic dilution system, a supporting table and a valve are arranged on the surface of the shell, a viscosity monitoring system, a temperature control system and a stirrer are arranged in the shell, and a wicking assembly connected with the supporting table through a telescopic assembly comprises a screening plate and a wicking bundle; the wicking beam adsorbs, separates and screens the cells by virtue of a wicking effect, a capillary action and an adhesion characteristic of the cells to the wicking beam, a stirrer is used for generating eddy current to stir liquid, and the whole cell adsorption screening and separation filtering process does not have too much mechanical friction, so that the problem of physical damage caused by cell separation is solved; the temperature control system can maintain a constant-temperature environment through a temperature sensor and a heating rod, the problem that the cell activity is reduced is solved, after adsorption screening, the shell can be subjected to separation and filtration operation after being cleaned, and the device is more flexible.

The invention provides an autoimmune disease physical therapeutic apparatus based on a microfluidic technology and dendritic cells, and belongs to the technical field of biomedical engineering. The therapeutic apparatus provided by the invention comprises an automatic blood sampling system, a target cell separation system, a micro-fluidic chip system, a high-precision liquid flow driving system, a real-time monitoring system, a cell feedback system and an intelligent control system which are connected in sequence. The therapeutic apparatus can integrally complete the whole process from patient blood sampling, target cell separation, physical regulation and control to cell reinfusion, and solves the problem that a safe, effective and controllable autoimmune disease physical therapeutic apparatus is lacked in the prior art.

The invention discloses a specific antigen activated immune cell complex and a preparation method and application thereof, the preparation method comprises the following steps: providing an HPV high-abundance antigen epitope fusion polypeptide, and connecting the HPV high-abundance antigen epitope fusion polypeptide with a cell penetrating peptide TAT to obtain a bifunctional polypeptide complex with cell penetrating and antigen specificity; the method comprises the following steps: providing mononuclear cells, separating to obtain dendritic cells, and activating the dendritic cells to obtain activated dendritic cells; co-incubating the bifunctional polypeptide compound and the activated dendritic cells, and collecting the cells to obtain the immune cell compound activated by the specific antigen. In combination with a test case, the immune cell compound activated by the specific antigen can realize administration through local smearing, so that the immune cell compound can directly act on infected epidermis, activate local immune response, effectively recognize and remove HPV infected cells, and achieve the effect of effectively treating HPV infection.

1. The name of the design product: cyclone centrifuge. 2. The use of the design product: the design product is used for reagent, solvent, chemical, cell separation, microbial sample separation and treatment. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The utility model relates to the technical field of reagent bottles, and discloses a reagent bottle for biological cell separation, which comprises a reagent bottle, a cover body is inserted into a bottle opening of the reagent bottle, a rubber ring is fixedly connected to the bottom of the cover body, and a fixing mechanism is arranged on the surface of the bottle opening of the reagent bottle. Through a fixing mechanism, after a reagent bottle is filled with a reagent, the reagent bottle is covered with a cover body, the bottom of a rubber ring is located at the top of the reagent bottle, then a pulling plate is pulled to enable a disc to move, then a connecting frame is rotated to enable the disc to rotate to the position above the cover body, then the pulling plate is loosened, and at the moment, the disc can move downwards through the elastic force of a spring; the cover body is tightly pressed by the bottom of the disc, so that the reagent bottle is tightly pressed by the rubber ring, the smell of a reagent in the reagent bottle does not fly out of the reagent bottle, a pungent smell is generated in a laboratory, and the personal safety of workers in the laboratory is further protected.

The invention provides a CTL (cytotoxic T lymphocyte) cell culture method. The method comprises the following steps: 1) pre-coating a cell culture flask; 2) plasma separation and inactivation; 3) separating peripheral blood mononuclear cells; 4) separating and washing mononuclear cells; and 5) activating and amplifying the cells. The method can further comprise the step (6) of further amplification of the cells. According to the method provided by the invention, 4-1BBL is introduced, so that the amplification multiple of the CTL cells can be effectively improved, the CTL cells can be massively proliferated in a short time, and the proportion of the CTL cells can also be improved. According to the invention, strict requirements of tumor immune cell therapy on CTL cell quality are met, and industrialized large-scale CTL cell preparation can be realized.