123 results about "Microscopic exam" patented technology

The invention discloses a full-automatic milk somatic cell detection system and method based on fluorescence analysis. According to the full-automatic fluorescence microscopic examination system, automatic sample introduction and automatic mixing are completed through the automatic sample introduction module, automatic focusing of dyed samples is completed through the automatic focusing module, automatic photographing processing and automatic counting are completed through the automatic photographing and counting module, and therefore full-automatic fluorescence microscopic examination is achieved. According to the method, the automatic detection level is remarkably improved, the consistency of the detection process is ensured, and manual operation errors are reduced, so that the experiment efficiency, the data accuracy and the reproducibility are improved; compared with traditional off-line detection or laboratory detection, the method and system provided by the invention have better real-time performance and accuracy, and also have higher automation degree.

The invention discloses a microbial spatial distribution monitoring method and system based on a multi-level microscopic scanning image, relates to the technical field of image processing, and is used for carrying out multi-level grid traversal type scanning image acquisition on an observation area of microscopic examination data. Identifying and counting the number of each target microorganism in each image and the position information of each target microorganism in the corresponding hierarchical grid, and storing the information into a to-be-identified hierarchical data set corresponding to each target microorganism; and according to the ecological type of each target microorganism, respectively inputting the corresponding to-be-identified hierarchical data set into the corresponding trained multi-organism distribution probability density model to obtain a spatial distribution rule of each microorganism in the microscopic examination data. By adopting multi-stage magnification grading scanning, the interaction and distribution rules among microbial species are respectively described according to microbial ecological types, and the accuracy of microbial distribution observation is improved.

The invention discloses a detection method of mycobacteria. The detection method comprises the following steps: S1, constructing a fusion protein gp23RD-GFP; s2, a target protein is obtained from the fusion protein gp23RD-GFP; s3, mixing the target protein with the mycobacterium culture, and incubating; s4, preparing a microscopic examination sample; s5, carrying out microscopic examination by using a fluorescence microscope; according to the method, a functional truncated body of phage TM4 receptor binding protein gp23 is fused with a green fluorescent protein to construct a fusion protein gp23RD-GFP, the fusion protein gp23RD-GFP is purified to obtain a target protein, the target protein and a mycobacterium culture are mixed and incubated, and finally the incubated mycobacterium culture is subjected to microscopic examination sample preparation. The detection mode is simple and rapid, and support can be provided for the joint development of the subsequent mycobacterium detection technology.

The utility model provides a device capable of automatically loading slides in batches, which comprises a slide table module, and the slide table module is used for providing a bearing platform for installing a cover glass mechanism and providing a slide preparation conveying platform capable of automatically preparing wet slides and stained slides in batches in sequence; the slide table module comprises a slide making table mechanism, a slide box positioning mechanism and a cover glass mechanism, the slide making table mechanism comprises a slide making table and a slide making table support, the slide making table comprises a slide making conveying platform and a cover glass mechanism placing table, and the slide making conveying platform comprises a wet slide making conveying table and a stained slide making conveying table. According to the utility model, a wet sheet and a stained sheet can be prepared for the same sample in sequence, so that the requirements of subsequent microscopic examination equipment for detecting the wet sheet and the stained sheet for the same sample are met, and the working efficiency and the accuracy of subsequent microscopic examination results are further improved.

The invention provides a multi-morphological prior fused pollen particle target detection system and method, and relates to the technical field of pollen detection, and the method comprises the steps: carrying out the feature extraction of a to-be-detected pollen particle image to obtain an initial feature map, and carrying out the convolution of the initial feature map through a pre-constructed morphological convolution kernel library to obtain a morphological response feature map; performing adaptive weighted feature fusion based on the morphological response feature map to obtain a morphological confidence map, and processing fusion features obtained based on the morphological confidence map and global context features extracted from the initial feature map to obtain a spatial attention map; and carrying out feature re-calibration on the initial feature map by using the space attention map to obtain an enhanced feature map, and then carrying out target detection on the enhanced feature map to obtain pollen particle information output. The method has the advantages that the problems that automatic pollen detection background interference is large and forms are difficult to distinguish are effectively solved, and high-time-efficiency pollen concentration monitoring is supported; and the defects that the traditional manual microscopic examination process is complicated, long in period and depends on professionals are overcome.

The invention discloses a nursing method before and after artificial spawning induction of parent mandarin fish, which comprises the following steps: nursing after collection of wild mandarin fish: selecting healthy, disease-free and residue-free fish, carrying out aeration transfer with water in a sorting box, controlling the temperature difference between water temperatures before and after transfer not to exceed 5 DEG C, soaking in a bag for over-temperature, soaking in saline water, and putting the wild mandarin fish into a 1: 1 filtered circulating water system; the steps of disinfection, pest killing, sterilization and bacteriostasis are alternately carried out, the ingestion condition, the swimming capacity and the clustering condition of the mandarin fish are observed, sampling microscopic examination observation is carried out, and if all conditions are normal and do not have parasitic diseases or bacterial infection, nursing work after the wild mandarin fish is collected is completed. The method further comprises the steps of prenatal breeding of mandarin fish, spawning induction, egg squeezing, postnatal breeding of parent fish and disease control, the survival rate of the wild mandarin fish after collection is improved in multiple aspects, breed conservation and breeding work of the wild mandarin fish is facilitated, the survival rate of the parent fish of the mandarin fish reaches 80% or above, and the method plays an important role in breeding of excellent new varieties which grow fast and are high in stress resistance.

The preparation method of the visible component microscopic examination sample comprises the following steps: preparing at least two samples with different light transmittances, and selecting at least two different sample treatment methods; comprising any two treatment methods as follows: a method A: for a light-proof to-be-detected sample, taking a set volume of light-proof sample, adding a set volume of water into the light-proof sample, and diluting to obtain an intermediate sample; taking the intermediate sample, and adding a reagent with a set volume or a set weight to obtain a microscopic examination sample; the method B comprises the following steps: for a to-be-detected semi-transparent sample, taking a semi-transparent sample with a set volume, and adding a reagent with a set volume or a set weight to obtain a microscopic examination sample; the method C comprises the following steps: enriching a light-transmitting sample with a set volume for a light-transmitting to-be-detected sample, and adding a reagent with a set volume or a set weight into an enriched intermediate sample with a set volume to obtain a microscopic sample. Corresponding methods are selected according to different light transmittances of the to-be-detected samples, so that the to-be-detected samples in different states can be prepared into microscopic examination samples suitable for efficient imaging of a microscope.

The invention relates to a method for identifying epitaxial stacking faults of heavily doped Ph products in advance, which belongs to the technical field of semiconductor epitaxial detection and comprises the following operation steps of: 1, preparing two groups of normal and abnormal batches of polished wafer samples, namely four groups of polished wafer samples, to participate in an experiment; 2, selecting one sample in the group A, one sample in the group C and one sample in the group D, and carrying out a corrosion test after first thermal oxidation; and 3, selecting one sample in the group B, one sample in the group C and one sample in the group D, carrying out secondary thermal oxidation, and then carrying out corrosion test. And 4, selecting a normal group of samples and an abnormal group of samples, and testing by using different thermal oxidation conditions and corrosion respectively. And 5, carrying out microscopic examination. And 6, identifying the silicon wafers with stacking faults in advance. The phenomenon of full-surface stacking fault after epitaxial processing of silicon wafers at some positions caused by thermal history difference of heavily doped Ph products is identified in advance. And the situation that the product is wasted due to the fact that the exception can be identified only through epitaxial processing is avoided.

The invention relates to a method for preparing a sample for microscopic examination comprising the steps of using a chitosan-based porous material having interconnected pores with sizes between 5 μm and 700 μm and a total porosity (volumetric fraction) between 40% and 90%, adding at least one biopsy sample containing tissue and / or eukaryotic and / or prokaryotic cells and / or eukaryotic cells containing viral particles to the chitosan-based porous material; and adding (C1-C6)-alkyl alcohol or mixtures thereof.

The utility model relates to the technical field of microscopic examination tool components, and discloses a reading circuit microscopic examination tool which is characterized in that the position of a reading circuit is limited or fixed through a plurality of limiting grooves formed in a microscopic examination tray, and the reading circuit is uniformly placed on the microscopic examination tray, so that the transmission distance is shortened, and the risk that the reading circuit falls off is avoided; in addition, the contact area between the back face of the reading circuit and the limiting groove can be reduced by arranging a plurality of stand columns, and contamination of the back face of the reading circuit is avoided.

In the preparation method of the visible component detection dry reagent, the redissolving reagent and the microscopic examination sample, the visible component detection dry reagent is formed by drying a liquid dyeing reagent; the liquid staining reagent comprises a staining agent and an acid-base regulator; the pH regulator is used for regulating the pH value of the liquid dyeing reagent; the liquid dyeing reagent is filtered before being dried. Before drying, dividing the liquid dyeing reagent into small parts, and drying separately; the liquid dyeing reagent is divided into single parts with set volume, and then is dried to remove moisture. The single part of the set capacity is 300 ul to 3000 ul per piece. The visible component is used for detecting the dry reagent, so that the reagent is more convenient to store and transport, and the quality guarantee period is longer.

This invention relates to the field of sputum smear cytology examination technology, and discloses an automated sputum cell analysis method and system based on deep learning. The method includes specimen collection and preprocessing, image preparation and acquisition, data preprocessing and dataset construction, improved YOLOv8 algorithm training, cell identification and statistics, large-scale model analysis, and report generation. This invention automates sputum cell analysis, significantly reducing specimen processing and interpretation time. The efficiency of each specimen analysis is several times higher than manual microscopic examination, reducing labor costs and efficiently handling the demand for large-scale specimen testing. The improved YOLOv8 algorithm, through the C2f-DCNv3 module, SPD-Conv, and color and texture attention mechanisms, accurately captures the differential characteristics of similar cells, significantly improving cell classification accuracy, reducing subjective and fatigue errors in manual interpretation, and resulting in more objective and reliable results.

The utility model provides a sampling vessel, and particularly relates to an under-microscope operation type multifunctional sampling vessel for vector parasites, which can be used for observing and collecting parasites under a microscope. The multifunctional sampling vessel comprises a transparent vessel body, a sampling tube, a collection suction tube and collection tweezers, a groove is formed in the vessel body close to the vessel wall, and the groove is used for placing and fixing the sampling tube; the lengths of the collecting suction tube and the collecting tweezers are smaller than the diameter of the dish body. The sampling vessel can improve the problem that the vector parasites are difficult to pick and collect during microscopic examination, improves the collection efficiency of the vector parasites and the accuracy of density measurement and calculation, can be used as a manufacturing container of a parasite soaking specimen, and has multiple functions.

The invention relates to the technical field of medical engineering, in particular to a microscope lifting support for medical engineering, which comprises a detection platform, a lifting rod, a microscope connecting frame, a bubble level, a connector, a positioning rod, a correction assembly and a balance base, the right side of the movable part is connected with a first control bolt, the left end of the movable part is fixedly provided with a microscope connecting frame, and the front end of the microscope connecting frame is connected with a second control bolt; according to the invention, through ingenious cooperation of the bubble level, the positioning rod and the correction assembly, the angle of the detection platform can be flexibly adjusted to realize rapid calibration, so that horizontal installation of the detection platform is ensured, and the problem that fluid detection objects overflow due to slight inclination of the object carrying platform is prevented; the support has the advantages of being flexible in control and correction, convenient to adjust and the like, and provides powerful support for microscopy in the field of medical engineering.

The invention discloses an inspection method for rapidly processing a wire rod metallographic specimen, and belongs to the technical field of metallographic detection. The invention provides an inspection method for rapidly processing a wire rod metallographic specimen in order to solve the problems of inconvenience in specimen processing operation marking, long inspection time, material mixing and material loss during hook-by-hook sampling inspection of a wire rod bearing steel rolling body product. The method comprises the steps of incoming material acceptance check, sample sorting, sample processing, metallographic sample preparation and microscopic examination, an auxiliary device is made of 45 # steel and is of a cuboid structure of 390mm * 75mm * 50mm, circular grooves are formed in the upper surface of the auxiliary device and are arranged in the width direction of the auxiliary device, every two circular grooves form one group, and ten groups of circular grooves are arranged; the diameter of each circular groove is 30 mm, the depth of each circular groove is 30 mm, and the gap between every two adjacent circular grooves is 5 mm. By adding an auxiliary device and a new technological process method, one-person operation can be reduced, the processing time is shortened by half, the probability of sample mixing and losing is 0%, and the accuracy and the efficiency of hook-by-hook inspection of the wire rod are greatly improved.

The invention belongs to the technical field of surgical instruments, and provides a body fluid sample collection device for hydrothorax and ascites and a use method thereof. A pretreatment device is arranged between a body fluid bottle and a suction pipeline and comprises a viscosity control part, the viscosity control part is provided with a conical pipe, the inner diameter of the end, close to the suction pipeline, of the conical pipe is smaller than that of the end, away from the suction pipeline, of the conical pipe, and branch pipe openings capable of being opened and closed are formed in the two ends of the conical pipe. The branch pipe openings comprise the first branch pipe opening and the second branch pipe opening, and the first branch pipe opening is closer to the suction pipeline than the second branch pipe opening. The problems that in the hydrothorax and ascites suction process, due to clots such as fibrin of high-viscosity hydrothorax and ascites, a suction pipeline is blocked, and the microscopic examination accuracy is affected are avoided; the problems that before and after the pleural and ascitic fluid needs to be sucked for multiple times and the pleural and ascitic fluid is indwelled and sucked, the pipeline is blocked due to residual clots in the pipeline, and even indwelling and suction instruments need to be replaced are avoided.

The invention discloses a method and device for identifying helicobacter pylori based on artificial intelligence fluorescence imaging, and relates to the technical field of artificial intelligence medical detection. Comprising the following steps: S1, carrying out slide preparation treatment on a cytological sample or a tissue slice sample, and processing the slide preparation through an immunofluorescent staining reagent to obtain a helicobacter pylori fluorescent staining picture; s2, outputting and obtaining a helicobacter pylori picture with a rectangular frame identifier through a helicobacter pylori identifier neural network model; and S3, according to the coordinate information of the rectangular frame, obtaining the coordinate information of the helicobacter pylori in the fluorescent staining picture, and splicing the helicobacter pylori pictures to obtain a spliced picture. According to the method, the helicobacter pylori is marked automatically through the helicobacter pylori identification neural network model, the problems of subjectivity and time consumption of traditional manual microscopic examination are avoided, meanwhile, the positions of thalli are accurately marked, positive probability data are output, and then the detection efficiency is improved.

According to the method and the device for detecting the unit volume number of the blood leukocytes and the leukocyte proportion, a microscopic examination sample is flatly laid, the flatly-laid height is H, an image is shot, the flatly-laid area SA of the corresponding microscopic examination sample in the image is selected, and the volume VA of the microscopic examination sample corresponding to the area SA is equal to SA * H; the microscopic examination sample volume VA is greater than the set minimum sample volume Vm; white blood cells in the selected image are identified, and the total number NA of the white blood cells in the selected image is obtained; the unit volume number of leucocytes in the microscopic sample is equal to NA / VA. Under the condition that red blood cells and white blood cells exist in a liquid-based blood suspension sample at the same time, it is ensured that an effective number of white blood cells can be obtained for analysis, and the result of white blood cell quantitative analysis is accurate and reliable.

This invention discloses a self-cleaning, anti-interference snail crushing device and its usage method for microscopic examination. The device includes a main body, a flat crushing unit, a snail placement unit, a pressure plate cleaning unit, and a snail placement plate detachably installed on the upper surface of the snail placement unit for placing and positioning snails. It employs automated pressure application technology, solving the technical problem that manual crushing methods inevitably cause uneven stress on the snails and varying degrees of crushing when handling a large number of snails for microscopic examination, thus affecting the quality of subsequent microscopic examinations. Furthermore, by integrating the pressure plate cleaning unit into the device, the pressure plate cleaning scheme is optimized, improving the efficiency of snail crushing. Simultaneously, multiple snail placement grooves are provided within the snail placement plate, effectively solving the problem of difficulty in neatly arranging snails during simultaneous crushing and microscopic examination of a large number of snails, and the technical problem of cross-contamination of fluids from adjacent crushed snails.

The invention discloses a blood microscopic imaging detection and blood evaluation method and device. The method comprises the following steps: preparing blood into a microscopic examination sample; adding a microscopic examination sample into the cavity of the detection chip; irradiating the detection chip cavity by using a light source A; shooting to obtain a microscopic examination sample image; detecting to obtain an image of a single red blood cell in the microscopic examination sample image, and obtaining the volume of the single red blood cell and the hemoglobin parameter of the single red blood cell according to the image of the single red blood cell; storing the volume and hemoglobin parameters of a single red blood cell; calculating the number of single red blood cells in the shot picture, and if the number of single red blood cells in the shot picture does not reach the set measurement number, moving the position of the detection chip cavity corresponding to shooting, changing the position of the X coordinate or the Y coordinate, and obtaining the volume of single red blood cells of red blood cells with the set measurement number and corresponding hemoglobin parameters.

Each of the multifunctional detection sample accommodating device and the multifunctional detection device comprises a detection part A and a detection part B; the detection part A comprises a cavity A; the cavity A is used for accommodating a sample A, the cavity A is used for shooting a microscopic magnification image of a microscopic examination sample, and the microscopic magnification image is used for visible component analysis; the detection part B comprises a cavity B; the cavity B is used for accommodating a sample B, the cavity B is used for optical detection analysis of the sample, and the optical detection analysis is used for detection-free analysis of the sample B. The multifunctional detection device comprises a microscopic amplification digital imaging assembly and an optical detection assembly, the microscopic magnification digital imaging assembly is used for shooting a microscopic magnification image of the microscopic examination sample; and obtaining an image of the sample in the cavity A for visible component analysis. Light in the optical detection assembly irradiates a sample in a cavity B of the external multifunctional detection device, and detection-free analysis of the sample is carried out according to optical reaction characteristics of the sample.

The system comprises detection equipment, the equipment is provided with a rack table top, a to-be-detected area is arranged on the rack table top, one side of the to-be-detected area is provided with a rotating mechanism, a mechanical arm, a blowing and sucking device and a line scanning camera array, the mechanical arm, the blowing and sucking device and the line scanning camera array are arranged on the periphery of the rotating mechanism, and a material carrying disc is placed in the to-be-detected area. The rotating mechanism is provided with a plurality of hollowed-out areas used for containing spinneret plates, and the hollowed-out areas correspondingly serve as a feeding station, a discharging station, a blowing and sucking cleaning station and a photographing detection station after the rotating mechanism rotates at a constant speed; the blowing and sucking device cleans micropores of the spinneret plate, the line scanning camera array collects images of the spinneret plate and detects the states of the micropores of the spinneret plate on the basis of a machine vision and machine learning method, and the mechanical arm grabs the spinneret plate to be detected to the feeding station and takes down the spinneret plate passing the detection from the discharging station and places the spinneret plate back to the original position of the material carrying disc. The automatic level of spinneret plate detection is effectively improved, human intervention is reduced, the detection efficiency is improved, and the detection cost is reduced.

This application relates to a method, apparatus, computer device, and storage medium for executing sample detection tasks. The method includes: receiving a sample detection instruction; responding to the sample detection instruction, sequentially determining whether there are any tasks to be executed in the task queue corresponding to each detection task type according to the priority order of each detection task type in a set of detection task types; if there are tasks to be executed in the current detection task type, then designating the tasks to be executed as target detection tasks; controlling a sample position transformer to perform position transformation according to the task execution position corresponding to the target detection task, so that the target detection sample corresponding to the target detection task is located at the task execution position, and executing the task operation corresponding to the target detection task at the task execution position. This method can improve the execution effect of microscopic examination tasks.

The invention discloses a multi-mode ultrasonic scanning and high-power microscopic examination fused plastic package device chip bonding layer layering judgment method, relates to the technical field of plastic package device defect detection, and aims at a plastic package device composed of a plastic package material, a chip, a bonding layer, a back exposed substrate and a lead frame. Firstly, the layering possibility below a chip is preliminarily judged through front C scanning, and T scanning is synchronously used for proving abnormity; the layering condition is verified from the depth dimension through back C scanning and B scanning, and each ultrasonic scanning mode is clear in parameter setting so as to guarantee the detection consistency; and finally, preparing a sample for the layered device, and visually confirming defects through microscopic examination of a 10000-times high-power microscope. According to the method, multi-mode ultrasonic scanning and high-power microscopic examination are fused, theoretical analysis and actual verification are combined, detection is comprehensive, judgment is accurate, and the method is not only suitable for chip bonding layer layering, but also can be used for defect judgment of plastic package material cavities, layering of substrates and plastic package materials and the like, and is wide in applicability.

The invention belongs to the field of bacterial detection of environmental microorganisms, and particularly relates to a detection method for in-situ labeling of tetrodotoxin in bacteria, which comprises the following steps: preparing a slice by using bacteria fixed by a solution containing methanol or ethanol, then carrying out pre-treatment of de-embedding and non-specific sealing, finally carrying out antigen-antibody reaction, and carrying out microscopic examination on a labeling signal by an electron microscope, so as to obtain the tetrodotoxin in-situ labeling detection of the tetrodotoxin in the bacteria. The qualitative and / or quantitative determination of the intracellular tetrodotoxin of the bacteria is realized. The method is high in specificity, good in repeatability and strong in observability, is used for detecting TTX-producing bacteria in the environment, and provides an effective and reliable means for researching the synthesis mechanism of the toxin in the bacteria.

The invention relates to a urine microscopic examination method, a chip and a system. The urine microscopic examination chip comprises a detection cavity A, wherein the cavity height of the detection cavity A is HA; the area of the detection cavity A corresponding to an image shot by the microscope is SA; the volume of the microscopic examination urine sample required by the detection item n is Vn; the number of images of different positions of the to-be-shot detection cavity A is greater than or equal to KAn; kAn = Vn / (SA * HA). And taking the volume Vn of the microscopic examination urine sample required by the detection item n as a lower limit digital basis for determining the number of shot images. In the urine microscopy chip, the volume of a detection cavity is determined according to the volume Vn of a microscopy urine sample required by a detection item n. In a urine detection system, the lower limit of the actual measurement volume TVn is determined according to the volume Vn of a microscopic examination urine sample required by a detection item n, so that the number of shot images is determined to be KKn; and the actual measurement volume TVn is obtained, and calculation is continued to obtain the number of detection items per unit volume = Pn / TVn.

The invention relates to the technical field of animal blood cell detection and analysis, in particular to a method for an animal blood cell analyzer to recognize platelet aggregation, which comprises the following steps: after the animal blood cell analyzer performs pretreatment and fluorescent staining on a blood sample in a white blood cell DIFF channel, acquiring three signals of blood cells through laser flow cytometry; two scatter diagrams are drawn according to the three signals, platelet aggregation particles are identified in a certain area range in the two scatter diagrams, when the number exceeds a threshold value, an alarm is given, and normal leukocytes cannot fall in the area. The good alarm function of the instrument provides more accurate prompts for clinical diagnosis, and clinical appropriate microscopic examination of platelets is facilitated. The cost is saved, extra testing is not needed, and the alarm function can be achieved in the leukocyte DIFF channel in the blood routine testing mode. The platelet aggregation region is identified, the influence of platelet aggregation on the total number of leukocytes is eliminated, and the leukocyte value is more accurate.

The invention discloses a preparation method of a monoclonal antibody for detecting trichophyton rubrum and application of the monoclonal antibody in fungal skin disease detection, and belongs to the field of biological medicine. The monoclonal antibody provided by the invention only has specific reaction with trichophyton rubrum, and has no cross reaction with common fungi such as trichophyton mentagrophytes, epidermophyton flocculent, candida albicans, malassezia and the like and normal flora of a human body, so that a false positive result is effectively avoided. Based on the double-antibody sandwich principle, the detection sensitivity can reach 200 pg / mL level, trace trichophyton rubrum in a clinical sample can be detected, and the positive rate is obviously higher than that of direct microscopic examination. The whole detection process can be completed within 15-20 min and is far faster than fungus culture for several weeks, a rapid diagnosis basis can be provided for doctors, and early treatment is facilitated. The kit provided by the invention has standardized operation steps, does not need complex instruments and special training, and is suitable for popularization and use in clinical laboratories of hospitals at all levels, outpatient clinics of dermatology and even basic medical institutions.

According to the visible component analysis method based on the microscopic examination sample, in the microscopic image acquisition process, shot pictures are divided into multiple groups, and each group of pictures comprises multiple layers of pictures in the Z-axis direction; a microscopic examination sample is irradiated by a light source A, K1 pictures are shot from bottom to top in the Z-axis direction when N groups of pictures are shot in the microscopic image acquisition process, focal power calculation is carried out on the K1 pictures, the picture position with the best focusing imaging is found out, and a picture for detection is shot at the picture position with the best focusing imaging; a microscopic examination sample is irradiated by a light source B, and in the microscopic image acquisition process, when N + 1 groups of pictures are shot, K2 pictures are shot from bottom to top in the Z-axis direction and are used as detection pictures. By means of the light source and the selection of the initial position of each group of images, an enough number of images are efficiently obtained, the images under different conditions and groups are respectively used for different parameter analysis, multiple purposes are achieved at one stroke, and the analysis efficiency is greatly improved.

The utility model discloses a pine wood nematode rapid detection device which is characterized in that a separator is arranged and is used for solving the problem of rapid separation of pine wood nematodes; separation of pine wood nematodes is facilitated through cooperation of the shell and the filtering disc, firstly, the pine wood nematodes needing to be separated are placed in the filtering disc, then water is injected to soak the pine wood nematodes, all samples are soaked in clear water for 24 hours, then pine wood is reserved on the filtering disc, the nematodes settle on a slope of the shell along with liquid, and then soaking liquid is discharged through the guiding and discharging pipe. The nematodes are discharged along with the liquid, the purpose of separating the pine wood nematodes is achieved, the nematodes in the subsequent soaking liquid can be subjected to natural sedimentation or centrifugation, redundant moisture is removed, the nematodes are concentrated to a proper amount for microscopic examination, then the top cover is opened, the filter disc is removed, and the pine wood nematodes are separated. After the pine wood on the filter disc is discharged, new pine wood needing to be separated is continuously placed, and the pine wood can be repeatedly recycled.