Paraffin section method for fern gametophytes

A paraffin section, fern technology, applied in the preparation of test samples, etc., can solve the problems of observing gametophyte anatomy, fern gametophyte loss of water, deformation, etc.

Inactive Publication Date: 2012-07-25
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional paraffin section technique will cause the fern gametophyte to lose water, severely deform and change the internal shape of the cell, and the anatomical structure of the gametophyte cannot be observed normally through the conventional paraffin section technique

Method used

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  • Paraffin section method for fern gametophytes
  • Paraffin section method for fern gametophytes
  • Paraffin section method for fern gametophytes

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Experimental program
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Embodiment Construction

[0013] The method adopted in the present invention is introduced in detail below.

[0014] 1. Material collection and fixation

[0015] When cultivating materials for the first time, determine the time range of each stage of sperm organ, archegonium, and embryo development according to the development time. FAA fixative, and stored in a refrigerator at 4 ° C, fixed in modified FAA fixative for 24 hours.

[0016] 2. Rinse

[0017] Rinse the fixed material with running water at a small flow for 1 to 2 hours, and then rinse with 30% alcohol, 50% alcohol, and 70% alcohol for 30 minutes each.

[0018] 3. Dyeing and bluing

[0019] Staining with Einstein's hematoxylin for 2 to 5 days, the cell nuclei turn reddish purple, and then rinse with tap water to make the cell nuclei dark blue and the cytoplasm light blue, and the bluing time is 3 to 5 hours; Gradient dehydration in 30% ethanol.

[0020] 4. Dehydration and transparency

[0021] Adopt ethanol step-by-step dehydration met...

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PUM

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Abstract

The invention discloses a paraffin section method for fern gametophytes, which comprises the steps of material selection and fixation, washing, coloration and bluing, dehydration and hyalinizing, waxing and embedding, sectioning, patching, dewaxing and mounting, as well as microscopic examination and photographing, wherein improved FAA (formalin, acetic acid and alcohol) stationary liquid is adopted in the fixation, and the stationary liquid consists of formalin, acetic acid and 30% alcohol (formalin:acetic acid:30% alcohol = 1:1:18). The method is adopted, so that the disadvantage of much youngness of the fern gametophytes can be effectively overcome; the fixation can be well performed for cell divisions at all stages; from a paraffin section in a later stage, a plurality of mitotic sections can be observed, so that a whole development process can be reflected detailedly; therefore, the method has high development and application values.

Description

technical field [0001] The invention relates to a method for paraffin sectioning of plants, in particular to a method for paraffin sectioning of fern gametophytes. technical background [0002] To date there is no technique specifically applied to the preparation of fern sections, and conventional paraffin sectioning techniques are commonly used to prepare dissected sections of ferns. However, the conventional paraffin section technique will cause the fern gametophyte to lose water, severely deform and change the internal shape of the cell, and the anatomical structure of the gametophyte cannot be observed normally through the conventional paraffin section technique. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for paraffin sectioning of fern gametophytes, including drawing and fixing, washing, dyeing and bluing, dehydration and transparency, wax dipping and embedding, sectioning, patching, dewaxing a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
Inventor 黄庆阳常缨樊锐锋
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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