9 results about "Paraffin section" patented technology

The invention discloses a genome sequencing sample processing method based on a microdissection technology. The method comprises the following steps: (1) preparing a paraffin section or a frozen section; (2) carrying out HE staining on the section obtained in the step (1); (3) carrying out laser microdissection on nucleic acid; (4) fragmenting the DNA sample, and treating a product obtained in the step (3) by utilizing an ultrasonication method to obtain a DNA fragment; a non-contact ultrasonic crusher is adopted, the power is 80%, ultrasonic treatment is carried out for 10 s, stopping is carried out for 20 s, and 15 cycles are carried out. And (5) carrying out on-machine pretreatment on the genome sequencing sample, wherein the treatment comprises tail end repair and joint connection treatment. According to the method disclosed by the invention, accurate cutting of the sample can be realized, a DNA sample is extracted from a very small amount of cells and a sample for sequencing is constructed, the sample can achieve the same or higher sequencing data quality as that of a traditional method, the usage amount of the cells is greatly reduced, and the repeatability of an experiment is improved.

The present invention addresses the problem of providing a laminate that is easily manipulated and results in little damage to an original paraffin block sample when a paraffin section sample is prepared, a production method for the laminate, a preparation method for a paraffin section sample, and a preparation method for a paraffin sample piece.　Provided is a laminate that includes a substrate, a first paraffin layer that is provided on at least a portion of the surface of the substrate, and a second paraffin layer that is provided on at least a portion of the first paraffin layer, the melting point T1 of a first paraffin that constitutes the first paraffin layer being higher than the melting point T2 of a second paraffin that constitutes the second paraffin layer.

The invention discloses a paraffin section mounting machine and method. The paraffin section mounting machine comprises a slicing device, a mounting water tank and a circulating device, an opening is formed in the top of the mounting water tank, and an inner cavity of the mounting water tank comprises a receiving area extending in the first direction and a waste discharging area extending in the second direction; the receiving area is communicated with the waste discharge area; the first direction and the second direction are perpendicular to each other and located in the same horizontal plane. A receiving notch is formed in the edge of one end, far away from the waste discharge area, of the receiving area, and is positioned below a blade of the slicing device; a shifting piece device and a first water filling nozzle connected with the circulating device are arranged at the receiving notch; a drainage notch and a second water injection port connected with the circulating device are respectively formed in two ends of the waste discharge area; the bottom surface of the bearing notch is higher than that of the drainage notch; and an inclined transition surface is arranged between the bottom surface of the bearing notch and the cutting edge. According to the device, waste pieces can be discharged, workers can be assisted to efficiently slice, pick and mount the pieces, and the working efficiency is improved.

The invention belongs to the technical field of plant paraffin sections, and discloses a preparation method of a paraffin section for analyzing the characteristics of xylem of a masson pine stem. The method comprises the following steps: (1) softening; (2) segmenting; (3) dehydrating; (4) transparentizing; (5) waxing; (6) embedding; (7) slicing, spreading, adhering and baking; (8) dewaxing and rehydrating; (9) dyeing and decolorizing; and (10) sheet sealing. According to the method, the preparation steps of the paraffin section are improved, specifically, the method comprises the steps of softener selection, wood part segmentation, dyeing time adjustment and the like, by adopting the technical scheme, the paraffin section with complete masson pine stem tissue and clear structure can be obtained, and the anatomical characteristics of the masson pine stem xylem are easy to observe and measure.

This invention relates to the field of paraffin sectioning technology and discloses a paraffin section pretreatment method suitable for plant spatial transcriptomics, comprising: (1) sample collection and fixation; (2) sample dehydration; (3) clearing; (4) paraffin embedding; (5) paraffin section preparation; and (6) pretreatment of sections before RNA FISH. This invention can be widely applied to various plant tissues, and its detection sensitivity can reach the visualization of low-expression genes (FPKM≤20), contributing to the popularization and development of plant spatial transcriptomics.

The paraffin slicing machine comprises a base, a box body, a paraffin fixing frame and a cutter fixing frame, a first rotating rod is arranged in the base in a rotating mode, second rotating rods are arranged in two second sliding grooves respectively, the first rotating rod is in transmission connection with the two second rotating rods through bevel gears respectively, and the cutter fixing frame is arranged in the box body. The two second rotating rods are in threaded connection with the two first sliding blocks; the box body is arranged on the base; the paraffin fixing frame is connected with one of the first sliding blocks; the tool fixing frame is connected with the other first sliding block, and the paraffin fixing frame and the tool fixing frame are driven to get close to each other or get away from each other at the same time through cooperation of the first rotating rod and the two second rotating rods. Different from the prior art, the positions of the paraffin fixing frame and the cutter fixing frame can be moved at the same time by rotating the first rotating rod, the operation time of the paraffin slicing machine is shortened, and the operation of the paraffin slicing machine is simplified.

The invention discloses a manufacturing method of a cinnamon vascular tissue paraffin section. According to the method, a self-adaptive gradient heating wax dipping technology is adopted, and the texture difference of cinnamon materials in different development stages from tender shoots to semi-lignified stems can be adapted. According to the method, the traditional chemical softening step is abandoned, the authenticity of the original components and the microstructure of the vascular tissue is completely reserved, and the slide preparation period is remarkably shortened. The paraffin section prepared by the method is high in tissue and cell morphology integrity, high in dyeing contrast and real. In addition, the method is reasonable in process design, effectively reduces dependence on expensive equipment, has universality and high efficiency, and solves the problems that the cell structure is damaged due to dependence on chemical softening and the requirements of soft and hard material slices are difficult to consider in the traditional method.

The invention discloses a paraffin section treatment device and method. The paraffin section processing device comprises a rack, a clamping mechanism arranged on the rack through a driving mechanism, a section sending mechanism used for placing a new glass slide, a sample box used for placing a sample glass slide, a slicing machine used for cutting a paraffin block, a section mounting water tank used for receiving a sample section and a circulating mechanism used for injecting water into an inner cavity of the section mounting water tank. The edge of the top opening is provided with a receiving gap and a drainage gap at intervals; the bearing notch is formed below the discharging end of the slicing machine, and the bottom face of the bearing notch is higher than the bottom face of the drainage notch. And the driving mechanism is suitable for driving the clamping mechanism to move, so that the clamping mechanism performs a sheet taking action, or a sheet mounting action, or a water throwing action, or a sheet sending action, or a sheet placing action. Through cooperation of the sample box, the sheet mounting water tank and all the mechanisms, automatic sheet taking, sheet mounting, sheet sending and other actions of the device are achieved, and the working efficiency is improved.

The invention provides a primer composition and a kit for grouping subjects based on ovarian support-interstitial cytoma and application of the primer composition and the kit. On the first aspect, the invention provides a primer composition which comprises a first primer, a second primer, a third primer, a fourth primer, a fifth primer and a sixth primer, and nucleotide sequences of the first primer, the second primer, the third primer, the fourth primer, the fifth primer and the sixth primer are respectively shown as SEQ ID NO: 1-6. A DNA sample of a tumor sample or a paraffin section is subjected to PCR amplification based on the primer composition, an amplification product is sequenced in combination with Sanger sequencing, and subjects are grouped based on a detection result, so that the defects of long time consumption and high requirements on specialty and experience of doctor reading of a traditional method are overcome, and the detection accuracy is improved. And a technical support is provided for clinical diagnosis of ovarian support-interstitial cell tumor.