22 results about "Paraffin section" patented technology

The invention discloses a genome sequencing sample processing method based on a microdissection technology. The method comprises the following steps: (1) preparing a paraffin section or a frozen section; (2) carrying out HE staining on the section obtained in the step (1); (3) carrying out laser microdissection on nucleic acid; (4) fragmenting the DNA sample, and treating a product obtained in the step (3) by utilizing an ultrasonication method to obtain a DNA fragment; a non-contact ultrasonic crusher is adopted, the power is 80%, ultrasonic treatment is carried out for 10 s, stopping is carried out for 20 s, and 15 cycles are carried out. And (5) carrying out on-machine pretreatment on the genome sequencing sample, wherein the treatment comprises tail end repair and joint connection treatment. According to the method disclosed by the invention, accurate cutting of the sample can be realized, a DNA sample is extracted from a very small amount of cells and a sample for sequencing is constructed, the sample can achieve the same or higher sequencing data quality as that of a traditional method, the usage amount of the cells is greatly reduced, and the repeatability of an experiment is improved.

The present invention addresses the problem of providing a laminate that is easily manipulated and results in little damage to an original paraffin block sample when a paraffin section sample is prepared, a production method for the laminate, a preparation method for a paraffin section sample, and a preparation method for a paraffin sample piece.　Provided is a laminate that includes a substrate, a first paraffin layer that is provided on at least a portion of the surface of the substrate, and a second paraffin layer that is provided on at least a portion of the first paraffin layer, the melting point T1 of a first paraffin that constitutes the first paraffin layer being higher than the melting point T2 of a second paraffin that constitutes the second paraffin layer.

The invention relates to a paraffin section detection method fusing Masson and HE dyeing and application, HE dyeing and Masson dyeing are carried out on the same specimen, and information of multiple aspects such as the basic structure of tissue cells, the dyeing condition of cell nucleuses and cytoplasm, the distribution condition of collagenous fibers and muscle fibers and the like can be obtained at the same time. Therefore, a more comprehensive and more accurate basis is provided for diagnosis and research of diseases. By adopting the method and the system, the diagnosis accuracy can be improved, more comprehensive and more accurate information is provided for disease research, and the occurrence mechanism, the development process and the treatment effect of the disease can be deeply known. The tedious process that different dyeing specimens need to be manufactured in a traditional method is avoided, and time and cost are saved. Meanwhile, continuous dyeing detection can be performed on the same specimen, so that the usage amount of the specimen is reduced, and the injury to a patient is reduced.

The invention discloses a detection reagent for detecting MHC-II expression of gastric cancer tissue tumor cells. The detection reagent comprises the following components in parts by mass: 10-30 parts of a paraffin section preparation solution, 2-10 parts of a dewaxing solution, 2-10 parts of a sodium citrate antigen repair solution and 20-40 parts of an immunohistochemical staining solution, the dewaxing liquid comprises xylene and at least one of a flavonol aminobenzoate derivative and erucyl amide benzoic acid benzyl trimethyl ammonium salt, the flavonol aminobenzoate derivative has a nitrogen-containing group and a benzene ring, and the erucyl amide benzoic acid benzyl trimethyl ammonium salt has long-chain unsaturated alkane and a nitrogen-containing group. The invention provides a detection reagent for detecting MHC-II expression of gastric cancer tissue tumor cells, and high-quality immunohistochemical staining sections can be obtained, so that the sensitivity and specificity of MHC-II expression detection are improved.

The invention belongs to the technical field of biomedical detection, and particularly relates to a standardized lung tissue microthrombus digital quantitative analysis method. Comprising the following steps: S1, embedding a left lung lobe of a complete mouse by using paraffin to obtain a wax block, slicing the wax block, and selecting a slice which can completely display each tissue structure of the lung at a lung door for dyeing and analysis; s2, paraffin sections are immersed, rinsed and subjected to antigen repair; blocking endogenous peroxidase of the paraffin section, and closing a non-specific binding site of the paraffin section to obtain a paraffin section tissue; s3, preparing a primary antibody working solution to carry out antigen-primary antibody reaction; preparing an HRP secondary antibody working solution and carrying out primary antibody-secondary antibody reaction; s4, carrying out DAB color development by using a DAB color development solution, re-dyeing the cell nucleus by using hematoxylin, and carrying out immersion, dip dyeing and dehydration treatment to obtain a transparent sealing sheet; s5, using a full-automatic slide scanner to obtain a lung tissue panoramic digital scanning picture; and S6, performing batch analysis on the mouse lung tissue microthrombus by using an HALO pathological image analysis platform.

The utility model relates to the technical field of calibration and positioning devices, in particular to a paraffin section calibration and positioning device which comprises a frame, a plurality of connecting rods, a gradienter and two clamping assemblies. The clamping assembly comprises a side plate, a spring, a pull block, a guide rod and a clamping plate; when the paraffin slicing machine is used, the two clamping assemblies are used for clamping the frame on the sample clamp, a doctor obtains the position A of the bubble by observing the position of the bubble on the gradienter so as to obtain the position of the transverse axis and the position of the longitudinal axis of the sample clamp, and when slicing is needed on another paraffin slicing machine, the frame can be clamped on the sample clamp on the other paraffin slicing machine, so that the paraffin slicing machine is convenient to use. The positions of the sample clamps are adjusted until the bubble position on the gradienter moves to the position A, at the moment, it can be guaranteed that the positions of the two sample clamps are consistent, the angle tangent planes of the sample clamps of all the paraffin slicing machines can be kept consistent, and waste of materials due to incomplete tangent planes of paraffin blocks is avoided.

The invention discloses a paraffin section mounting machine and method. The paraffin section mounting machine comprises a slicing device, a mounting water tank and a circulating device, an opening is formed in the top of the mounting water tank, and an inner cavity of the mounting water tank comprises a receiving area extending in the first direction and a waste discharging area extending in the second direction; the receiving area is communicated with the waste discharge area; the first direction and the second direction are perpendicular to each other and located in the same horizontal plane. A receiving notch is formed in the edge of one end, far away from the waste discharge area, of the receiving area, and is positioned below a blade of the slicing device; a shifting piece device and a first water filling nozzle connected with the circulating device are arranged at the receiving notch; a drainage notch and a second water injection port connected with the circulating device are respectively formed in two ends of the waste discharge area; the bottom surface of the bearing notch is higher than that of the drainage notch; and an inclined transition surface is arranged between the bottom surface of the bearing notch and the cutting edge. According to the device, waste pieces can be discharged, workers can be assisted to efficiently slice, pick and mount the pieces, and the working efficiency is improved.

The invention discloses a paraffin section manufacturing method for research on development characteristics of arundo donax rhizomes and adventitious buds of the arundo donax rhizomes, and belongs to the technical field of paraffin sections. (2) fixing; (3) softening with a 15% HF solution; (4) gradient alcohol dehydration; (5) the xylene is transparent; (6) waxing and embedding; (7) slicing the wax block to obtain a wax belt with the thickness of 10 microns, and pasting and drying the wax belt; (8) dewaxing; (9) dyeing with sarranine and fast green dyes; and (10) sheet sealing. According to the method, the preparation steps of the paraffin section are improved, specifically, the steps of softener selection, section thickness determination, dyeing time determination and the like are included, the section quality is improved, the section with a complete and clear tissue structure and clear dyeing can be cut out, and therefore the microstructure of the rhizome of the bamboo reed and the adventitious bud of the rhizome of the bamboo reed can be observed conveniently; reliable technical support can be provided for research on formation, development and differentiation of bamboo reed rhizomes and adventitious buds of the bamboo reed rhizomes.

The invention discloses a preparation method and application of an Asian cotton root tip paraffin section. The preparation method comprises the following steps: S21, culturing an experimental material; s22, taking and fixing experimental materials; s23, dehydrating the experimental material; s24, transparency of the experimental material; s25, carrying out wax dipping on the experimental material; s26, embedding the experimental material; s27, performing wax repairing on the experimental material; s28, preparing hybridized slices of experimental materials; the prepared section can be used for carrying out positioning research on the Asian cotton root tip gene by adopting an RNA in-situ hybridization method, the method has very high sensitivity and specificity, and the obtained hybridization section result can be stored for a long time.

The invention belongs to the technical field of plant paraffin sections, and discloses a preparation method of a paraffin section for analyzing the characteristics of xylem of a masson pine stem. The method comprises the following steps: (1) softening; (2) segmenting; (3) dehydrating; (4) transparentizing; (5) waxing; (6) embedding; (7) slicing, spreading, adhering and baking; (8) dewaxing and rehydrating; (9) dyeing and decolorizing; and (10) sheet sealing. According to the method, the preparation steps of the paraffin section are improved, specifically, the method comprises the steps of softener selection, wood part segmentation, dyeing time adjustment and the like, by adopting the technical scheme, the paraffin section with complete masson pine stem tissue and clear structure can be obtained, and the anatomical characteristics of the masson pine stem xylem are easy to observe and measure.

This invention relates to the field of paraffin sectioning technology and discloses a paraffin section pretreatment method suitable for plant spatial transcriptomics, comprising: (1) sample collection and fixation; (2) sample dehydration; (3) clearing; (4) paraffin embedding; (5) paraffin section preparation; and (6) pretreatment of sections before RNA FISH. This invention can be widely applied to various plant tissues, and its detection sensitivity can reach the visualization of low-expression genes (FPKM≤20), contributing to the popularization and development of plant spatial transcriptomics.

1. Name of the Design Product: Paraffin Sectioning Machine (Dual-screen Display). 2. Use of the Design Product: Used for making paraffin sections of pathological tissues, or cutting plant rubber, etc. 3. Design Key Points of the Design Product: Lies in the shape. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective View 1.

The invention provides a preparation method of a paraffin-embedded section of decalcified bone tissue, which belongs to the field of paraffin section manufacturing and comprises the steps of fixation, decalcification, gradient concentration ethanol treatment, embedding, freezing, slicing and the like. The method for treating the glass slide is simple and low in cost, a uniform film can be formed, the section keeps good adhesiveness in the embedding and dyeing process, meanwhile, the solvent resistance is good, the problem of section falling caused by xylene dewaxing is solved, tissue loss can be remarkably reduced, and the experiment stability and repeatability are improved. In addition, a temperature gradient wax dipping method is also used, so that the permeation rate of paraffin inside and on the surface of the tissue is more uniform, a uniform and stable paraffin embedding matrix is finally formed, the slice quality is improved, and the dyeing and microscopic observation effects are clearer.

The paraffin slicing machine comprises a base, a box body, a paraffin fixing frame and a cutter fixing frame, a first rotating rod is arranged in the base in a rotating mode, second rotating rods are arranged in two second sliding grooves respectively, the first rotating rod is in transmission connection with the two second rotating rods through bevel gears respectively, and the cutter fixing frame is arranged in the box body. The two second rotating rods are in threaded connection with the two first sliding blocks; the box body is arranged on the base; the paraffin fixing frame is connected with one of the first sliding blocks; the tool fixing frame is connected with the other first sliding block, and the paraffin fixing frame and the tool fixing frame are driven to get close to each other or get away from each other at the same time through cooperation of the first rotating rod and the two second rotating rods. Different from the prior art, the positions of the paraffin fixing frame and the cutter fixing frame can be moved at the same time by rotating the first rotating rod, the operation time of the paraffin slicing machine is shortened, and the operation of the paraffin slicing machine is simplified.

The invention discloses a manufacturing method of a cinnamon vascular tissue paraffin section. According to the method, a self-adaptive gradient heating wax dipping technology is adopted, and the texture difference of cinnamon materials in different development stages from tender shoots to semi-lignified stems can be adapted. According to the method, the traditional chemical softening step is abandoned, the authenticity of the original components and the microstructure of the vascular tissue is completely reserved, and the slide preparation period is remarkably shortened. The paraffin section prepared by the method is high in tissue and cell morphology integrity, high in dyeing contrast and real. In addition, the method is reasonable in process design, effectively reduces dependence on expensive equipment, has universality and high efficiency, and solves the problems that the cell structure is damaged due to dependence on chemical softening and the requirements of soft and hard material slices are difficult to consider in the traditional method.

The slice drying instrument comprises a drying instrument body, a heat preservation side plate is fixedly connected to the outer side of the drying instrument body, a heat preservation gasket is fixedly connected to the inner end of the heat preservation side plate, the heat preservation side plate is connected with the drying instrument body through the heat preservation gasket, and a constant-temperature notch is formed in an inner cavity of the heat preservation gasket. The paraffin section drying device comprises the heat preservation side plates, in the process that a user dries paraffin sections through the drying plate, the drying plate is heated through the heating plate firstly, and then heat preservation treatment is conducted on the drying plate through the heat preservation side plates symmetrically arranged on the two sides; the inner sides of the heat preservation side plates are connected with the drying plate through the heat preservation gaskets, then air circulation in the heat preservation gaskets is guaranteed through the constant-temperature notches, and finally the heat preservation effect is achieved through the heat preservation side plates, the heat preservation gaskets and the constant-temperature notches, so that the temperature of the upper surface of the drying plate is not prone to loss, and the practicability of the drying instrument is improved.

The present application belongs to the technical field of paraffin section, and particularly relates to a preparation method of a woody dicotyledon root system paraffin section. The preparation method of the woody dicotyledon root system paraffin section provided by the present application comprises the following steps in sequence: fixing, sampling, dehydration and pre-staining, secondary transparency, wax immersion, embedding, block trimming, cementing, sectioning, section spreading and sticking, dewaxing, rehydration, staining, dehydration and transparency. The preparation method of the present application is closely matched with each step, and is connected with the previous step and the next step. The preparation method of the present application can significantly improve the success rate of root system tissue sectioning while shortening the sectioning time, and the section is complete, clear and easy to observe, which can effectively solve the technical problem that the plant root system of different development degrees and the regional structure difference are too large to be sectioned in the prior art.

The invention discloses a paraffin section treatment device and method. The paraffin section processing device comprises a rack, a clamping mechanism arranged on the rack through a driving mechanism, a section sending mechanism used for placing a new glass slide, a sample box used for placing a sample glass slide, a slicing machine used for cutting a paraffin block, a section mounting water tank used for receiving a sample section and a circulating mechanism used for injecting water into an inner cavity of the section mounting water tank. The edge of the top opening is provided with a receiving gap and a drainage gap at intervals; the bearing notch is formed below the discharging end of the slicing machine, and the bottom face of the bearing notch is higher than the bottom face of the drainage notch. And the driving mechanism is suitable for driving the clamping mechanism to move, so that the clamping mechanism performs a sheet taking action, or a sheet mounting action, or a water throwing action, or a sheet sending action, or a sheet placing action. Through cooperation of the sample box, the sheet mounting water tank and all the mechanisms, automatic sheet taking, sheet mounting, sheet sending and other actions of the device are achieved, and the working efficiency is improved.

The utility model relates to a paraffin section preservation box, which belongs to the field of histological section preparation tools and comprises a box body and a box cover, a support is arranged in the box body, a plurality of section racks distributed in a linear array are arranged on the support, one side of each section rack is rotatably connected with the support, paraffin section glass slides are carried by the section racks in a two-end clamping mode, and the paraffin section glass slides are arranged on the box cover. Each slicing frame comprises a side plate, the middle section of each side plate is of a vertical narrowing structure, clamping blocks are arranged at the two ends of each side plate, a groove of a V-shaped structure is formed in each clamping block, the support comprises a supporting rod and two protection plates, all the slicing frames are located between the two protection plates, shifting blocks are arranged on the sides, away from the supporting rods, of the slicing frames, and the shifting blocks on every two adjacent slicing frames are distributed in a staggered mode. According to the utility model, the operation convenience of taking and placing the paraffin section glass slide is improved by arranging the section frame which can be independently screwed out.

The present invention provides a method for preparing paraffin sections of mantis shrimp eyeballs, comprising the following steps: step one: sampling; step two: fixation selection: fix with 4-6% paraformaldehyde at room temperature for 25-35 minutes; step three: dehydration treatment; step four: embedding the wax-impregnated tissue block with a modified paraffin wax; step five: continuously sectioning the sample using a microtome; step six: flattening the slices in a 37°C water bath; step seven: dewaxing and rehydrating after incubation at 37°C for 6-12 hours; step eight: after staining the sample, sealing the sample with a 50% sterile glycerol aqueous solution. The method for preparing paraffin sections of mantis shrimp eyeballs of the present invention comprises the improvement and optimization of procedures such as the dissection of the mantis shrimp eyeball shell, the selection of a fixative, dehydration, and waxing, breaking through the technical bottlenecks such as incomplete dehydration and waxing caused by the difficulty of dissecting the mantis shrimp eyeball due to the shell being difficult to dissect, damage to the blade due to the hard shell, and fragmentation of the slices.

The invention discloses a paraffin section pasting method capable of being used for space transcriptomics and application, and belongs to the technical field of paraffin sections.The paraffin section pasting method comprises the following steps that sample paraffin blocks needing to be researched are cut into paraffin rolls; a round knife is used for buckling and pressing the wax roll, and a target tissue area is cut; inflating the tail part of the circular knife through an injector, and blowing out a cut complete target tissue piece; adsorbing the complete target tissue piece by using a needle head with static electricity; and heating the space transcription chip, dropwise adding ultrapure water into a target patch area on the space transcription chip to form liquid drops, enabling a needle head with static electricity adsorbed with the complete target tissue slice to approach the target patch area, and attracting the complete target tissue slice onto the liquid drops under the electrostatic action to complete paraffin section patch. According to the method, the wax roll is used for splicing a plurality of samples, damage to the wax block is avoided, the manufacturing process is simple, high technical level and professional equipment are not needed, and therefore the manufacturing cost is low.

The invention provides a primer composition and a kit for grouping subjects based on ovarian support-interstitial cytoma and application of the primer composition and the kit. On the first aspect, the invention provides a primer composition which comprises a first primer, a second primer, a third primer, a fourth primer, a fifth primer and a sixth primer, and nucleotide sequences of the first primer, the second primer, the third primer, the fourth primer, the fifth primer and the sixth primer are respectively shown as SEQ ID NO: 1-6. A DNA sample of a tumor sample or a paraffin section is subjected to PCR amplification based on the primer composition, an amplification product is sequenced in combination with Sanger sequencing, and subjects are grouped based on a detection result, so that the defects of long time consumption and high requirements on specialty and experience of doctor reading of a traditional method are overcome, and the detection accuracy is improved. And a technical support is provided for clinical diagnosis of ovarian support-interstitial cell tumor.