1174 results about "Embryo" patented technology

The invention is suitable for the technical field of biological materials, and provides an embryo heavy ion track acquisition method based on a biological stack, and the method comprises the steps: carrying out the preprocessing of an initial biological stack, obtaining a target biological stack, obtaining an initial scanning image of the target biological stack, and scanning the target biological stack, and obtaining an initial scanning image of the target biological stack; marking the initial scanning image for the first time based on the central position of the seed embryo to obtain a target scanning image; fitting the target scanning image with the detection sheet of the target biological stack to obtain an initial fitting piece, and marking on the non-contact surface of the initial fitting piece for the second time according to the central position of the embryo to obtain a target fitting piece; scanning the target fitting piece through a preset laser confocal microscope to obtain an imaging picture of the seed embryo, and cutting an actual projection of the seed embryo on a preset projection contour based on the imaging picture and the central position of the seed embryo in the imaging picture; and identifying the heavy ion track of the seed embryo according to the actual projection.

The present invention discloses a semi-artificial diet for Tuta absoluta larvae, a preparation method thereof and an application thereof. In the present invention, 1000 mL of water is boiled, and after it is stable, 0.5 - 1 g of sorbic acid, 0.5 - 1 g of sodium p-hydroxybenzoate and 0.1 - 0.2 g of thiabendazole are added, and quickly stirred and melted. Subsequently, compound vitamins are added, and after stirring evenly, 500 - 550 g of malt embryos are added and stirred into a dough. When the temperature drops below 30 °C, 150 - 200 g of tomato leaf powder is added and stirred and mixed evenly to obtain the semi-artificial diet for Tuta absoluta larvae. The components of the semi-artificial diet of the present invention can not only meet the nutritional requirements of test insects, but also have an optimal compound ratio of different components, and particularly give the best nutritional ratio. At this ratio, the survival rate, pupation rate and emergence rate from larvae to pupae are high, and the deformity rate of pupae is low.

The invention discloses a gene for improving wheat immature embryo transformation efficiency and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a new wheat regeneration gene TaDJA gene is found, and the TaDJA gene is over-expressed in a wheat variety 'Kefeng No.3' which is extremely difficult to transform, so that the callus induction rate and the transformation efficiency of wheat immature embryos can be improved; the method is beneficial for integrating exogenous nucleic acid molecules into the genome of the wheat variety difficult to transform, and provides technical support for genetic improvement of the wheat variety difficult to transform.

The invention discloses application of a ZmFAH1 gene in regulating and controlling low temperature resistance of corn in a germination period, a related molecular marker and application of the molecular marker. The invention firstly provides the high-low-temperature-resistant ZmFAH1 gene, and the relative expression quantity of the high-low-temperature-resistant ZmFAH1 gene is obviously higher than that of a low-temperature sensitive material in embryos in a germination stage, buds in a bud stage, roots in the bud stage and roots in a seedling stage; the ZmFAH1 gene can significantly improve the low temperature resistance of corn in the germination period, and also can enhance the low temperature resistance of corn in the germination period and the seedling period. The invention further provides a molecular marker DNCAPS708 which is located at the 708 -710 sites of the CDS region of the negative chain ZmFAH1 gene of the first chromosome of the corn, and three deleted basic groups are GAA / <->. The research finds that the overexpression of the ZmFAH1 gene can effectively improve the low temperature resistance of the corn, which is of great significance to the molecular biology mechanism research of the low temperature resistance function of the corn.

The invention discloses a corn germplasm resource viability cross-germplasm discrimination method, system, equipment and medium, and relates to the technical field of corn viability discrimination, in the method, hyperspectral data of an embryo area and hyperspectral data of an endosperm area are obtained; obtaining hyperspectral data of each sample corn seed; each piece of hyperspectral data is preprocessed; feature selection is carried out on each piece of preprocessed hyperspectral data, and a constructed double-branch spectrum Transform corn germplasm cross-germplasm viability detection model is trained in combination with viability label data of each sample corn seed; the trained double-branch spectrum Transform corn germplasm cross-germplasm viability detection model is utilized to discriminate the cross-germplasm corn to be discriminated, the viability prediction result of the cross-germplasm corn to be discriminated is obtained, and the accuracy and reliability of cross-germplasm corn germplasm resource viability discrimination can be remarkably improved.

The present invention provides: a drug which can improve the implantation rate of an embryo and can be used in conjunction with conventional drugs for infertility treatment; and a screening method and the like with which it is possible to reliably evaluate such a drug. Specifically, the integrin activator comprises: (1) a peptide composed of KFEERMRCKWMT, and (2) a peptide composed of KFEERMRCKWMT. (2) a peptide which is composed of KFEEERSRCKWMT; (3) a peptide comprising an amino acid sequence obtained by deleting, substituting and / or adding 1-3 amino acids in (1) or (2); or (4) a peptide obtained by binding any one of (1) to (3) to a membrane-permeable molecule. Provided are an artificial uterus and a screening method based on an implantation reproduction test system using a fertilized egg (for example, an embryo developed to an implanted blastocyst), the artificial uterus comprising: a hydrogel containing endometrial stromal cells and having an extracellular matrix as a main component; and a uterine epithelial organoid configured to be surrounded by the hydrogel.

The invention discloses a corn ZmMYBR37 gene and an application of the corn ZmMYBR37 gene in regulating and controlling the size of corn kernels. The nucleotide sequence of the ZmMYBR37 gene is as shown in SEQ ID No: 1 in a sequence table. A mutant material with small grains and reduced hundred-grain weight is separated from a corn EMS mutant library, and a mutation site occurs on a function unknown gene ZmMYBR37, so that protein translation is terminated in advance. The information analysis finds that the gene coding protein has a conservative MYB structural domain, and the gene coding protein is speculated to belong to an MYB transcription factor family. Gene expression analysis finds that the gene is high in constitutive expression in corn and high in expression level in leaves and embryos. The corn material overexpressed with the ZmMYBR37 has the advantages that the grain size is increased, and the hundred-grain weight is obviously increased, so that the ZmMYBR37 has the potential of increasing the corn yield.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a device and a method for gradually adjusting the glucose concentration of an in-vitro embryo culture solution. The device comprises at least two miniature liquid storage bins, wherein each miniature liquid storage bin is filled with a high-concentration glucose solution in advance; the slow release channel is respectively connected with each micro liquid storage bin and the culture solution main container; the culture solution main container is used for accommodating an embryo and a basal culture medium; the control unit is used for sequentially opening the release ports of the miniature liquid storage bins, so that the high-concentration glucose solution sequentially flows into the culture solution main container through the slow release channels. The technical means of staged closed-loop micro sugar control is introduced into the structure and the using method of the device, so that the accurate adjustment of the sugar concentration of the in-vitro embryo culture solution is realized, the requirements of embryos in different development stages can be effectively met, and the embryo culture quality and the subsequent development potential are remarkably improved.

The invention provides an AbAIL5 gene and application of the AbAIL5 gene in improvement of genetic transformation efficiency of amorphophallus bulbifer, and belongs to the technical field of biology. The invention provides an AbAIL5 gene. The nucleotide sequence of the AbAIL5 gene is shown as SEQ ID NO: 1. Genetic transformation is performed on the amorphophallus bulbifer somatic embryo by constructing an overexpression vector of the AbAIL5 gene, so that the genetic transformation efficiency of the amorphophallus bulbifer callus can be improved, and candidate genes are provided for application of a large-scale breeding technology and a genetic transformation technology of the amorphophallus bulbifer callus in good varieties.

Disclosed in the present invention is a use of alisol-B23-acetate (AB23a) in the preparation of a drug for preventing or treating hypertrophic cardiomyopathy and diseases caused by the hypertrophic cardiomyopathy. The present invention provides for the first time a use of AB23a in the prevention or treatment of hypertrophic cardiomyopathy. According to the present invention, use of AB23a in direct in vitro treatment of cardiomyocytes derived from directed differentiation of human embryonic stem cells revealed that AB23a-based in-vitro treatment can significantly inhibit hypertrophic phenotypes of the human embryonic stem cells-cardiomyocytes; using an AB23a-containing feed to feed mice with hereditary hypertrophic cardiomyopathy caused by a gene mutation revealed that AB23a prominently relieves pathological myocardial hypertrophy of the mice and improves the cardiac function; AB23a can be used for preparing a drug for resisting hereditary hypertrophic cardiomyopathy, offering a novel way and means for treating hypertrophic cardiomyopathy; and AB23a is the main medicinal ingredient in the traditional Chinese medicinal herb-Rhizoma Alismatis, is safe to organisms, and has good clinical application prospects.

The invention provides application of a sperm tRNA (transfer ribonucleic acid) derived fragment 5 'tRF-Glu-CTC in prediction of development potential of assisted reproductive embryos. The expression of a tRNA derived fragment 5 'tRF-Glu-CTC in a sperm sample is detected, the abnormal expression of the sperm specific tRNA derived fragment 5' tRF-Glu-CTC in a DFI increase patient is verified, and the analysis on the correlation with clinical data and reproductive outcome of the patient prompts that the 5 'tRF-Glu-CTC molecular marker has significant correlation with the reproductive outcome, so that the 5' tRF-Glu-CTC molecular marker can be used for detecting the expression of the sperm specific tRNA derived fragment 5 'tRF-Glu-CTC in the DFI increase patient. The embryonic development potential can be effectively predicted, and a biomarker is provided for assisted reproduction treatment. In different experimental groups, the 5 'tRF-Glu-CTC can significantly improve the prediction precision, and shows good prediction performance in different clinical data. The detection method based on the fragment is expected to become a new tool for evaluating the assisted reproduction curative effect, the reproduction treatment scheme is further optimized, and the success rate is improved.

The invention discloses an artificial cultivation method of a hybrid of epinephelus trifoliatus and epinephelus lanceolatus, which comprises the following steps: taking healthy epinephelus trifoliatus as female parent fish, and carrying out cultivation strengthening and artificial ripening to obtain strengthened female parent fish; healthy epinephelus lanceolatus is used as male parent fish, and sexual mature male parent fish is obtained through natural sexual maturity; the ovulation time and the spermiation time are consistent; collecting egg liquid and seminal fluid, inseminating to obtain fertilized eggs, and performing embryo incubation and illumination regulation and control cultivation after opening to obtain hybrid spots; carrying out authenticity identification on the hybrid spots by utilizing a primer pair, and determining that the hybrid spots inherit genetic information of the female parent fish and the male parent fish; the primer pair is a sequence pair composed of SEQ ID NO. 1 and SEQ ID NO. 2, and a sequence pair composed of SEQ ID NO. 3 and SEQ ID NO. 4. The technical blank of crossbreeding by taking the epinephelus trifoliatus as the parent epinephelus is filled, and the authenticity of the hybridized spots is reliable.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated or applied with a nuclease protein, guide RNA or RNP for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

The invention relates to the technical field of bioinformatics, in particular to an embryo gene detection and analysis system for data storage. Comprising a signal standardization unit, a data preprocessing unit, a storage optimization unit, a machine learning analysis unit, an intelligent report unit and a safety calculation unit. Through intelligent segmentation, parallel computing and SIMD optimization of the data preprocessing unit, the processing efficiency and quality of original sequencing signals are greatly improved, and meanwhile, the machine learning analysis unit utilizes a generative adversarial network and auto-encoder fusion model, a transfer learning and meta-learning strategy and a multi-modal fusion technology; the deep mining of gene data features, the improvement of model generalization ability and the effective integration of multi-source data are realized, so that the embryo health is accurately evaluated, the disease prediction accuracy is improved, and the accuracy, robustness and clinical application value of the system are remarkably enhanced.

The invention discloses an in-vitro fertilization-based method and system for predicting the cumulative live productivity of multiple complete cycles, and the method comprises the steps: obtaining clinical data, embryo information and pregnancy follow-up visit data of a patient, and screening and preprocessing a sample in combination with a collection and discharge standard; feature variables are extracted before treatment, after treatment and at the early stage of pregnancy, an optimal set is determined, and key factors influencing live birth at all stages are accurately captured; after the nonlinear variables are processed by the RCS, a pre-treatment / post-treatment COX regression model and a Logistic regression model are adaptively constructed, and the prediction precision is improved by fitting data characteristics; and meanwhile, by evaluating the iterative optimization model, the variable weight is visually displayed by using a Nomogram graph after the model is qualified. The method can accurately predict the multi-cycle cumulative live productivity, assists a doctor in formulating a personalized scheme, helps a patient to reasonably plan treatment, reduces the time and economic burden of blind treatment, and has clinical practical value and benefits of the patient.

Disclosed herein are non-human animals (e.g., rodents, e.g., mice or rats) genetically engineered to express a human or humanized T cell receptor (TCR) from a human or humanized TCR locus comprising a non-human TCR non-coding sequence, and optionally a humanized T cell co-receptor (e.g., humanized CD4 and / or CD8 (e.g., CD8α and / or CD8β)), and / or a human or humanized major histocompatibility complex that binds the humanized T cell co-receptor (e.g., human or humanized MHC II (e.g., MHC II α and / or MHC II β chains) and / or MHC I (e.g., MHC Iα) respectively, and optionally human or humanized β2 microglobulin). Also provided are embryos, tissues, and cells expressing the same. Methods for making the genetically engineered animals are also provided. Methods for using the genetically engineered animals for developing human therapeutics are also provided.

The invention discloses a dairy cow timed insemination method for controlling ovulation time and improving embryo implantation rate, and relates to the technical field of dairy cow breeding. The problems that follicles on ovaries of postpartum cows are not synchronous in development, ovulation time cannot be accurately regulated and controlled, and embryonic development regulation and control after fertilization are lacked are solved. The method mainly comprises the following steps: three times of gonadotropin releasing hormone injection, two times of cloprostenol sodium injection, gonadotropin releasing hormone injection, luteinizing hormone releasing hormone A3 injection, timed artificial semen deposition, and VADE mixed liquid injection. GnRH is used for actively regulating follicular development, 95% or more of the dairy cows are synchronized after 2-3 rounds of starting, then timing insemination is performed, the conception rate of the postpartum dairy cows can be remarkably increased, compared with the prior art, the pretreatment time is short, the synchronous ovulation rate and conception rate of the dairy cows can be increased, the calving interval is shortened, and the survival rate of the dairy cows is increased. The economic benefit of the pasture is obviously increased, and the breeding management level can be improved on the basis.

The invention relates to the technical field of biomedicine detection reagent manufacturing and reproductive medicine, in particular to an embryonic development potential sperm key protein kit and a use method thereof. The method is characterized in that an antibody combination capable of specifically recognizing a group of key proteins related to embryonic development potential in human sperms is prepared, and the antibodies, a fluorescence labeled secondary antibody, a cell nucleus dye, a concentrated washing liquid, a mounting medium and a pre-coated control porous glass slide are assembled into the special detection kit. The kit can be combined with a multi-mode imaging system (such as a fluorescence / confocal microscope and a common optical microscope) and image analysis software to realize the traditional analysis of sperm morphology and movement ability, and can perform synchronous and accurate qualitative and quantitative analysis with the expression level and subcellular localization of the key protein. The invention provides an integrated molecular detection solution, and provides a powerful tool for sperm quality screening and embryonic development potential prediction in an assisted reproduction technology.

The invention provides a milk protein peptide which takes milk protein as a raw material and has the effects of promoting calcium absorption and improving bone health and application of the milk protein peptide. Specifically, milk protein peptide sequences, including YGGVSL and SQRY, are screened by adopting enzymolysis, sequence identification and molecular docking methods. The milk protein peptide is proved to have high calcium transport promoting capacity in the in-vitro intervention of Caco-2 cells, and the milk protein peptide is proved to have the capacity of promoting osteogenesis and inhibiting bone resorption in the in-vitro intervention of MC3T3-E1 mouse embryo osteoblasts. In animal experiments, by increasing the content of blood calcium and bone calcium, reducing the content of excrement calcium, improving mRNA expression of calcium ion channel protein, calcium binding protein and peptide transporter in intestinal tracts, enhancing the efficiency of calcium entering blood, promoting osteogenesis and inhibiting bone resorption, the milk protein peptide is comprehensively proved to have the capabilities of improving calcium resorption of organisms and improving bone health. The milk protein peptide has both functionality and nutrition, and has a wide application prospect in the aspect of bone health.

The invention belongs to the technical field of biological medicine, and particularly relates to a construction method of a heat-syndrome zebrafish model, the model and application. The construction method comprises the following steps: adding a hot drug into a fluorescent zebrafish embryo for induction; the preparation method of the heat medicine comprises the following steps: S1, weighing traditional Chinese medicine heat syndrome whole-formula medicinal materials; s2, after water is added, heating and boiling are conducted, and suction filtration is conducted after reflux extraction; s3, repeating the step S2 twice; s4, combining the three filtrates, and freeze-drying after rotary evaporation to obtain traditional Chinese medicine heat syndrome whole-formula powder; and S5, dissolving to obtain the hot medicine. According to the construction method, the number of macrophages at the inflammation part of the zebra fish is remarkably increased, so that immune overexpression is caused, and the heat-syndrome zebra fish model is successfully obtained. The construction method disclosed by the invention is simple and convenient to operate, short in modeling time, high in experimental efficiency and easy to observe modeling indexes, and can be used for researching pathogenesis and treatment methods of the heat syndrome, screening drugs or vaccines for preventing or treating the heat syndrome and testing the safety and effectiveness of the drugs.

The invention discloses an electrotransfection solution for pig in-vitro fertilization embryos and application thereof, and belongs to the technical field of pig embryo in-vitro editing. The objective of the invention is to solve the technical problems of low in-vitro electrotransformation efficiency and large damage of pig embryos. The invention provides an electrotransfection liquid for a pig in-vitro fertilized embryo. The electrotransfection liquid is prepared from the following components: a basic liquid which is Opti-MEM, N-acetylcysteine, mannitol and a small-molecule inhibitor Y-27632. According to the technical scheme, the embryo damage in the electrotransfection process can be remarkably reduced while efficient delivery of the gene editing tool is ensured, and a key technical support is provided for establishing a stable and efficient pig embryo gene editing platform.

The invention discloses a method for establishing a mature embryo callus induction and regeneration system of siberian wildrye seeds, which comprises the following steps of: screening the siberian wildrye seeds collected in the field, removing fungi on the seeds, and disinfecting the siberian wildrye seeds; after the disinfected seeds are longitudinally cut, the seeds are sequentially put into an induction culture medium to be subjected to induction culture, a subculture medium to be subjected to subculture, a differentiation and proliferation culture medium to be subjected to differentiation and proliferation culture and a rooting culture medium to be subjected to rooting culture, and to-be-differentiated seedlings with the root length being 4-5 cm are obtained; moving the to-be-differentiated seedlings out of the rooting culture medium, closing the bottle for seedling hardening, cleaning roots, injecting tap water, opening the bottle for seedling hardening, and culturing by using sterilized vermiculite to obtain complete regenerated plants. According to the method, the growth of the plant callus is remarkably improved, rapid, efficient and genetically stable plant propagation is realized, and a basic experimental material is provided for molecular breeding of the Elsholtzia sibirica.

A method of analysing a sample of a plurality of fish oocytes (100) is disclosed The method comprises, for each oocyte in the sample: capturing a first set of images of the oocyte from a plurality of rotational angels when rotated about a first axis of rotation; capturing a second set of images of the oocyte from a plurality of rotational angels when rotated about a second axis of rotation, which second axis of rotation is orthogonal to the first axis of rotation; identifying at least one characteristic (104) of lipid droplet coalescence in the captured first and second sets of images; and based on the identified at least one characteristic of lipid droplet coalescence, classifying the oocyte as belonging to one of a plurality of predefined categories, and based on the distribution of the sampled oocytes in the plurality of categories, attributing a quality parameter to the sample of oocytes, the quality parameter being any one of: a predicted fertilisation rate of the sampled oocytes; a predicted eyed-embryo success rate of the sampled oocytes; and a predicted hatching success rate of the sampled oocytes. A computer-implemented method of attributing a quality measure to a sample of fish oocytes is also disclosed, as is a related computer program and a related oocyte analysis apparatus. A computer-implemented method for training a neural network for use in a system for analysing a sample of fish oocytes is also disclosed.

The invention relates to the field of computer vision, in particular to a bovine embryo quality evaluation method and system based on a time sequence microscopic image, and the method comprises the steps: collecting a microscopic image and a timestamp, carrying out the embryo segmentation and inter-frame registration of the image, and obtaining the frame quality weight, morphological characteristics, image block embedding and topological data; respectively calculating a topological distance and an optimal transmission distance, determining a change point generation event sequence in combination with a frame quality weight, and aligning and outputting a penalty term based on a time Petri network; inferring a stage sequence and a quality posteriori based on the event sequence and a penalty term, and constructing a multi-instance set based on an event time window to obtain a multi-instance learning score; and fusing quality posteriori and multi-instance learning score to obtain an evaluation result, calculating a conflict coefficient, and relocating an event time window when a threshold value is exceeded to update the evaluation result. According to the method, rating consistency, noise immunity and traceability are improved.

The invention discloses application of an FGF9 gene in regulation and control of growth and development of chicken skeletal muscles, and further discloses application of an overexpression vector of the FGF9 gene or an interference fragment of the overexpression vector in promotion or inhibition of proliferation and differentiation of chicken myoblasts. The invention also discloses a specific primer pair for detecting the expression quantity of the FGF9 influencing the growth and development of the chicken skeletal muscle. The invention also discloses a detection kit and a detection method thereof. The detection kit provided by the invention can be used for detecting the expression quantity of the FGF9 gene in chicken muscle tissues and myoblasts, and the detection method is simple and quick. The FGF9 gene can be used as an important gene for researching a chicken skeletal muscle growth and development regulation theory.

The present invention relates to a method for obtaining a genetically modified progeny of an avian animal by packaging a gene edited or genetically modified vector in a recombinant adenovirus and injecting into the dorsal aorta of an embryo when PGCs migrate to a developing gonad; compared with the existing disclosed method for transforming the PGCs in vitro, the operation method disclosed by the invention not only can stably and effectively infect the PGCs in vivo, but also has the advantages that more importantly, the probability of generating gene editing offspring is higher, and particularly, the breeding efficiency of cultivating the transgenic chicken offspring with KRT75L4 gene and MSTN gene knockout is higher; and on the other hand, the reporter gene or crispr-cas protein gene does not need to be integrated into the genome of the bird and the non-native protein does not need to be expressed, so that the operability is higher from the commercial level.

The invention discloses a mouse embryo quality and development stage combined prediction system and method based on deep learning, and the system comprises a data preprocessing module which is used for obtaining a time sequence data set, and a feature extraction module which is used for processing the time sequence data set through a pre-trained OfficientNet model, and obtaining the feature vector of an embryo image corresponding to each time point; the time sequence feature fusion module is used for processing the image feature vectors of all embryos arranged according to the time sequence by using a bidirectional long-short-term memory network to obtain hidden state vectors of all embryo image fusion time sequence information; and the multi-task prediction module is used for inputting the hidden state vector into the embryo quality prediction and development stage classification prediction model after joint optimization to obtain a splicing result of embryo quality prediction and development stage classification prediction. According to the method, two prediction tasks of embryo quality and development stage classification can be processed at the same time by utilizing the combination of the OfficientNet model and the bidirectional long-short-term memory network, so that the model processing efficiency is improved.

The invention relates to the technical field of gene engineering, in particular to a transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on a Tol2 transposition subsystem and a construction method of the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry. The preparation method comprises the following steps: S1, constructing a Tol2 transposon expression vector pTol2-ef1a-tjp1a-P2A-mCherry which contains an ef1a promoter, a tjp1a gene, a P2A peptide sequence and an mCherry reporter gene; s2, carrying out in-vitro transcription to prepare Tol2 transposase mRNA; s3, mixing the expression vector with Tol2 transposase mRNA, and microinjecting the mixture into the single-cell stage embryo of the zebra fish; s4, performing fluorescence screening on the F0 generation embryos surviving after injection to obtain the Founder fish with positive transgenosis; s5, after the F0-generation positive fish is bred to be sexually mature, an F1 generation is obtained through mating, transgenic positive individuals with stable inheritance are screened out after identification, and a transgenic zebrafish strain is established. According to the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on the Tol2 transposon system and the construction method of the transgenic zebrafish system pTo12-efla-tjp1a-P2A-mCherry based on the Tol2 transposon system, efficient integration of exogenous genes is achieved by utilizing the Tol2 transposon system, and the transgenic zebrafish line capable of being stably inherited to the F1 generation is successfully obtained.