1831 results about "Embryo" patented technology

The invention relates to the technical field of database construction, in particular to a construction method and system for a reproductive medicine database. The method comprises the following steps: acquiring a biological tissue sample of a patient; acquiring reproductive endocrine hormone data, embryo morphological parameter data, immune cell function data and genome variation data from the biological tissue sample of the patient; performing time sequence analysis processing on the hormone fluctuation mode according to the reproductive endocrine hormone data to obtain endocrine dynamic characteristic data; performing image recognition processing based on coherent superposition characteristics of light on the embryo morphological parameter data, and performing embryo development potential evaluation to obtain embryo development potential data; and performing hierarchical clustering processing on the individual heterogeneity of the patient according to the immune cell function data and the genome variation data to obtain immune-genetic typing data of the patient. By means of the quantum dot sensing technology and the multi-channel micro-fluidic chip, standardization of data acquisition is achieved, and the heterogeneity problem of a data source is solved.

The invention discloses an embryo growth stage prediction method and system based on self-supervised learning, and the method comprises the following steps: collecting embryo images at different growth stages as a training set, and segmenting each embryo image in the training set into a plurality of blocks with the same size; randomly selecting half of the blocks from each embryo image for mask processing to obtain a mask training set; a prediction model comprising an encoder and a decoder is constructed, the encoder extracts a feature sequence from the mask training set, and the decoder reconstructs an embryo image according to the feature sequence; the kernel functions of the multiple weighted combinations serve as loss functions of the prediction model, the weight of each kernel function is adjusted through cross validation and gradient descent, parameters of the prediction model are adjusted and optimized through back propagation, and an optimal prediction model is obtained; and inputting a to-be-predicted embryo image into the optimal prediction model to obtain a growth stage prediction result of the embryo.

The invention discloses a deep learning method and system for multi-stage classification of embryonic development, and belongs to the field of multi-stage classification of embryonic development, and the method comprises the steps: carrying out the partitioning operation of a preprocessed embryo image, inputting the segmented image into a dual-branch local feature fusion module, and carrying out the local feature extraction, and obtaining a feature map containing fine-grained local features; inputting the feature map containing the fine-grained local features into a deep neural network module for global feature extraction and coding to obtain a final feature map; and inputting the final feature map into a classifier for development stage prediction, and outputting a predicted embryonic development stage. The advantages of the dual-branch local feature fusion module and the deep neural network module are combined to be used for classification of the embryo development stage, local and global information of the embryo image can be fully extracted, the accuracy of the classification stage is improved, and meanwhile a new thought is provided for application of deep learning in IVF.

The invention provides an automatic focusing method based on embryo image sequences, electronic equipment and a storage medium. The method comprises the following steps: inputting a plurality of groups of embryo image sequences into an automatic focusing network for training; the automatic focusing network comprises a Transform module, a channel attention mechanism module and a feature fusion module; the Transform module is used for capturing global information of the embryo image sequence through a self-attention mechanism and outputting a feature vector; the channel attention mechanism module is used for adjusting the weight of each embryo image in the embryo image sequence and outputting a feature vector; the feature fusion module is used for fusing the output of the Transform module and the output of the channel attention mechanism module; and inputting a to-be-focused embryo image sequence into the trained automatic focusing network to obtain a clearest embryo picture in the embryo image sequence. And in combination with time sequence analysis, the morphological change in the embryo development process is tracked, and more comprehensive data support is provided for embryo selection.

The invention relates to the field of gene editing, in particular to a peony PoLEC1 gene and application of protein coded by the peony PoLEC1 gene. The peony PoLEC1 gene is cloned from cultivated peony paeonia ostii, after the peony PoLEC1 gene is transformed into arabidopsis thaliana, phenotypic contrast finds that the peony PoLEC1 gene can play a role in bolting and flowering as well as growth and development of plants, specifically, overexpression of the peony PoLEC1 gene can increase the number of leaves, delay bolting and flowering time and reduce the height of the plants. According to the invention, arabidopsis thaliana is taken as a model plant, and a certain theoretical basis is provided for establishing a complete plant regeneration and genetic transformation system for peony by exploring the functional characteristics of the peony PoLEC1 gene and the expression characteristics of the peony PoLEC1 gene in the whole somatic embryo direct generation process and in each tissue of overexpressed arabidopsis thaliana.

Provided are compositions for genome editing and site-directed integration in plants comprising microprojectile particles coated, treated of applied with a recombinant DNA construct comprising a sequence encoding one or more genome editing reagents for delivery to a mature embryo explant from dry seeds. Further provided are methods for genome editing and site-directed integration in at least one cell of a plant using the disclosed compositions, and plants, plant parts and seeds comprising an edited genome or site-directed integration, which are produced by the disclosed methods.

The invention belongs to the technical field of edible oil preparation, particularly relates to a camellia oil preparation system and a preparation method, and aims to solve the problems that when an existing camellia oil preparation system prepares a pressed blank before squeezing, the amount of added crushed camellia materials cannot be accurately mastered, so that the pressed blank needs to be repeatedly squeezed for multiple times after being formed, and the production cost is reduced. In order to solve the problems that in the prior art, the number of times of completely squeezing a pressed blank cannot be effectively confirmed, and the squeezing efficiency and effect are reduced, according to the scheme, the device comprises a barrel, a cover plate is fixedly connected to the inner wall of the barrel, a stirring module is arranged on the cover plate, a round hole is formed in the cover plate, and a feeding hopper is fixedly connected into the round hole. According to the camellia oil preparation system and the preparation method disclosed by the invention, the quantity of crushed camellia particles in the pressed blanks for squeezing the camellia oil can be accurately controlled, so that the forming quality of the pressed blanks is effectively controlled, the camellia oil can be completely squeezed by repeatedly squeezing each pressed blank for the same times, the repeated operation is greatly reduced, and the production efficiency is improved. And the oil pressing efficiency is improved.

The invention discloses a construction method of an agrobacterium tumefaciens mediated masson pine genetic transformation system, and belongs to the technical field of genetic engineering. According to the construction method of the agrobacterium tumefaciens-mediated masson pine genetic transformation system, immature masson pine cones are collected, embryogenic calluses are induced and cultured, the embryogenic calluses of the masson pine in a PEM III stage are used as a material, suspension pre-culture is performed to obtain an acceptor material, and the acceptor material is subjected to genetic transformation to obtain the agrobacterium tumefaciens-mediated masson pine genetic transformation system. The method comprises the following steps: firstly, carrying out tissue culture on a transgenic somatic embryo, infecting by adopting an agrobacterium infection solution with an OD600 value of 0.2-0.6, carrying out co-culture, recovery culture and screening culture, carrying out histochemical staining by adopting GUS, carrying out molecular identification to obtain a transgenic embryogenic callus, and carrying out maturation treatment to finally obtain the transgenic somatic embryo. The masson pine genetic transformation system established by the invention is stable in transformation performance and high in transformation positive rate, and when an acceptor material is masson pine embryonic callus with the genotype of 1-3-5, the transformation positive rate is as high as 100%.

The invention is applicable to the technical field of biotechnology and genetic engineering, and provides an agrobacterium rhizogenes mediated genetic transformation method for hairy roots of blueberries. According to the method, a blueberry perennial stem segment is used as a receptor material for infection, and rapid genetic transformation of the blueberry stem segment is realized through an agrobacterium tumefaciens-mediated method. The method ingeniously bypasses the lengthy process of differentiating the explants to form somatic embryos, and compared with a traditional leaf disc method genetic transformation system, the method has the advantage that the genetic transformation period is remarkably shortened. And the method can be used for mass infection. Even in a non-sterile environment, the method can realize high-efficiency transformation of the gene, and shows the characteristics of simplicity and high efficiency. Therefore, the invention not only provides a new way for genetic transformation of the blueberries, but also provides valuable reference for genetic transformation research and gene function research of the blueberries.

The invention discloses a pig chimeric embryo culture medium and application thereof in effectively improving the in-vitro chimeric ability of pig embryonic stem cells, and belongs to the technical field of bioengineering. According to the pig chimeric embryo culture medium disclosed by the invention, a neutral culture environment for promoting synchronous development of stem cells and embryos is created by accurately regulating and controlling growth factors, nutrient substances and other key components in the culture medium. On the basis, overexpression of anti-apoptosis factors, optimization of microinjection conditions and adoption of a laser-assisted incubation means are combined, so that the chimeric efficiency is further improved, integration of the stem cells and embryos is effectively supported, the chimeric efficiency is improved, and a reliable in-vitro tool is provided for evaluating the chimeric ability and pluripotency of the pig stem cells.

The invention is suitable for the technical field of biological materials, and provides an embryo heavy ion track acquisition method based on a biological stack, and the method comprises the steps: carrying out the preprocessing of an initial biological stack, obtaining a target biological stack, obtaining an initial scanning image of the target biological stack, and scanning the target biological stack, and obtaining an initial scanning image of the target biological stack; marking the initial scanning image for the first time based on the central position of the seed embryo to obtain a target scanning image; fitting the target scanning image with the detection sheet of the target biological stack to obtain an initial fitting piece, and marking on the non-contact surface of the initial fitting piece for the second time according to the central position of the embryo to obtain a target fitting piece; scanning the target fitting piece through a preset laser confocal microscope to obtain an imaging picture of the seed embryo, and cutting an actual projection of the seed embryo on a preset projection contour based on the imaging picture and the central position of the seed embryo in the imaging picture; and identifying the heavy ion track of the seed embryo according to the actual projection.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a method for establishing an elymus nutans seed callus induction and regeneration system. The invention provides a tissue culture medium and a tissue culture method for culturing elymus nutans, and in the tissue culture medium provided by the invention, 2, 4-D can induce explants to form calluses and promote cell division and growth. The CH has a good effect on differentiation of embryoid and adventitious buds. ABA can promote the development of seed embryos. The KT can promote the generation of calluses and the growth of adventitious buds. NAA can promote plant growth and promote generation of adventitious roots. Under the combined action of NAA, 2, 4-D, ABA, KT and CH, the callus induction rate of the mature embryo is improved. The invention also provides a regeneration tissue culture method of elymus nutans, the mature embryo is used as an explant for tissue culture, and the callus induction rate of the mature embryo is high.

The invention belongs to the field of stem cells, and particularly relates to a method for inducing porcine embryonic stem cells to differentiate skeletal muscle cells by using a complete small molecule compound. The method comprises the following steps: S1, an induction stage: culturing the porcine embryonic stem cells in a culture medium for two days, and replacing with culture medium culture in the induction stage; s2, a differentiation stage: a differentiation stage culture medium is used for culture. The method is simple in operation process and high in efficiency, and large-scale production of the cell meat is facilitated while differentiation of the porcine embryonic stem cells into the skeletal muscle cells is achieved.

The invention relates to the technical field of water conservancy and hydropower ecological protection, in particular to an inhabiting structure based on different ecological habits of fishes and a habitat building method. The base body comprises a vertical plate and a cover top plate, and the cover top plate is formed by bending the upper end of the vertical plate towards the downstream direction; side plates are arranged at the two ends of the base body respectively. The vertical plate, the cover top plate and the two side plates jointly define a fish inhabitation chamber with an open downstream end; a lower partition plate and an upper partition plate are arranged in the fish inhabiting chamber to divide the fish inhabiting chamber into an embryonic larva fish layer, a juvenile fish layer and a water level fluctuation layer from bottom to top; a groove plate is arranged in the embryonic larva fish layer; a plurality of grooves are formed in the groove plate, and the bottom of each groove is filled with a substrate; a plurality of plant lattice tables are arranged on the top surface of the trough plate; a square plant table is arranged on the top face of the lower partition plate, and aquatic plants are planted in the plant lattice tables and the square plant table.

The invention provides an intelligent crop seed vigor detection method and system. The method comprises the following steps: collecting and preprocessing seed data; extracting appearance, spectrum, hardness and internal quality characteristics of the seeds to generate a characteristic set; and inputting the feature set to the vigor prediction model to obtain a vigor prediction value, and dynamically adjusting a classification threshold to classify seed vigor. According to the method, quadruple features are integrated to construct a high-dimensional feature set, and the physiological activity and mechanical integrity of the seeds are accurately represented through a nanoindenter and an X-ray CT technology. An error feedback and optimization method is introduced, data changes are responded in real time, and the classification accuracy is improved. Micron-scale quantification is realized through X-ray CT tomography, and the embryo development evaluation accuracy is improved. The time sequence neural network is constructed, the dynamic relation between the characteristics is captured, the seed vigor descending trend is predicted in advance, and the long-term prediction stability is better.

The invention discloses a novel corn haploid immature embryo tissue culture doubling method, and belongs to the field of plant breeding. The technical problem to be solved by the invention is how to obtain a simpler corn haploid breeding method. The corn haploid immature embryo chromosome doubling and seedling forming method provided by the invention comprises the following steps: taking a corn inbred line as a female parent, taking a corn haploid induction line as a male parent, inducing to obtain a corn immature embryo, inoculating the corn immature embryo into a double-layer culture medium, and culturing to obtain the corn haploid immature embryo chromosome doubling and seedling forming method. Obtaining seedlings formed by chromosome doubling of the corn haploid immature embryos; the double-layer culture medium is composed of a seedling culture medium and a doubling culture medium located on the seedling culture medium. According to the method provided by the invention, chromosome doubling and seedling formation of the maize haploid immature embryos can be completed in one step, and the maize haploid immature embryos do not need to be transferred to a seedling formation culture medium after chromosome doubling, so that a large amount of manpower is saved, and the method is expected to be used in production practice.

The invention discloses a nano-carrier for gene editing of aquatic animals. The nano-carrier comprises a) TNP nano-particles and b) Cas9 mRNA and sgRNA. The invention also discloses an application of the TNP nano-particle as an mRNA delivery carrier and a gene editing method based on the nano-carrier, according to the method, the TNP nano-particle with good biocompatibility is used for packaging Cas9 mRNA and sgRNA, and an efficient and low-damage gene editing delivery mode is realized. The nano-carrier is delivered into an embryo of an aquatic animal in a non-invasive manner, so that the transfection efficiency and the specificity of gene editing are optimized. In addition, the method can improve the gene editing efficiency and precision and enhance the embryo survival rate, is simple, convenient and safe to operate and wide in applicability, can support customized gene editing, and is beneficial to promoting the application of gene editing in aquatic product germplasm genetic improvement.

The invention relates to a rice embryo specific expression transfluorescent protein gene and Malt gene editing integrated vector and a preparation method thereof, the vector comprises a gene editing fragment and a fluorescent protein gene fragment, the sequence of the gene editing fragment is shown as SEQ ID NO.1; the sequence of the fluorescent protein gene segment is as shown in SEQ ID NO. 2; when the vector is applied to a molecular detection technology, a rice induction line with a homozygous embryo-specific expression transfluorescent protein gene and a mutant gene can be screened out, and the time for creating the induction line with the embryo-specific expression transfluorescent protein gene is saved, so that the cost is saved.

The invention relates to a method for producing notoginsenoside and ginsenoside by utilizing panax notoginseng cotyledon embryos, and belongs to the technical field of plant adventitious root tissue culture. The method comprises the following steps: inducing embryogenic calluses by utilizing immature zygotic embryos of panax notoginseng; proliferating the panax notoginseng embryogenic callus on a plate culture medium; s2, putting the pseudo-ginseng embryogenic callus obtained in the step S2 into a liquid culture medium, carrying out cell dispersion, and sieving by using a sieve with the aperture of 24-30 meshes; a mechanical stirring type bioreactor is filled with a liquid culture medium, and the globular embryo and the heart-shaped embryo are placed in the mechanical stirring type bioreactor to be cultured; s3, recovering the filtrate in the step S3, filtering by using a screen with the aperture of 30-80 meshes and 80-150 meshes to obtain uniform panax notoginseng embryogenic cells, and differentiating the panax notoginseng embryogenic cells in a triangular flask for the step S4; and drying the cotyledon embryo obtained in the step S4 at 45 DEG C, and grinding into powder. According to the method, large-scale generation of cotyledon embryos can be completed only in 4-6 weeks, the production process is remarkably accelerated, the method is suitable for industrial batch production requirements, and the production efficiency is high.

The present invention discloses a semi-artificial diet for Tuta absoluta larvae, a preparation method thereof and an application thereof. In the present invention, 1000 mL of water is boiled, and after it is stable, 0.5 - 1 g of sorbic acid, 0.5 - 1 g of sodium p-hydroxybenzoate and 0.1 - 0.2 g of thiabendazole are added, and quickly stirred and melted. Subsequently, compound vitamins are added, and after stirring evenly, 500 - 550 g of malt embryos are added and stirred into a dough. When the temperature drops below 30 °C, 150 - 200 g of tomato leaf powder is added and stirred and mixed evenly to obtain the semi-artificial diet for Tuta absoluta larvae. The components of the semi-artificial diet of the present invention can not only meet the nutritional requirements of test insects, but also have an optimal compound ratio of different components, and particularly give the best nutritional ratio. At this ratio, the survival rate, pupation rate and emergence rate from larvae to pupae are high, and the deformity rate of pupae is low.

The invention discloses a gene for improving wheat immature embryo transformation efficiency and application thereof, and belongs to the technical field of plant genetic engineering. According to the invention, a new wheat regeneration gene TaDJA gene is found, and the TaDJA gene is over-expressed in a wheat variety 'Kefeng No.3' which is extremely difficult to transform, so that the callus induction rate and the transformation efficiency of wheat immature embryos can be improved; the method is beneficial for integrating exogenous nucleic acid molecules into the genome of the wheat variety difficult to transform, and provides technical support for genetic improvement of the wheat variety difficult to transform.

The invention relates to the technical field of freeze-dried powder preparation, and discloses a white tomato exosome freeze-dried powder preparation method, which comprises: 1, manually picking white tomatoes 3-5 days after a color changing period, carrying out double-frequency ultrasonic cleaning, and pre-cooling in a 4 DEG C phosphate buffer solution containing 0.1% of vitamin C; according to the method, by virtue of an ultrasonic-cellulase synergistic crushing process, cells are mildly and efficiently crushed, so that the damage to an exosome structure is reduced, and the release efficiency and activity of the exosome are improved; the zebra fish embryo melanogenesis can be obviously inhibited, and the whitening and freckle-removing effects are relatively good.

The invention provides a tissue culture method for inducing cluster buds of paeonia delavayi embryo, and relates to the technical field of tissue culture of paeonia delavayi. The tissue culture method mainly comprises the steps of explant acquisition, embryo germination culture, embryo cluster bud induction culture, cluster bud multiplication culture, cluster bud rooting culture, acclimatization and transplantation and the like. According to the method, the defects in the prior art are overcome, the embryos in fresh and mature Paeonia delavayi seeds in the current year are used as explants, a regeneration system of an embryo cluster bud induction way is preliminarily established through embryo cluster bud induction, proliferation and rooting culture medium research, and a foundation is laid for establishment of a Paeonia delavayi genetic transformation system.

The invention discloses application of a ZmFAH1 gene in regulating and controlling low temperature resistance of corn in a germination period, a related molecular marker and application of the molecular marker. The invention firstly provides the high-low-temperature-resistant ZmFAH1 gene, and the relative expression quantity of the high-low-temperature-resistant ZmFAH1 gene is obviously higher than that of a low-temperature sensitive material in embryos in a germination stage, buds in a bud stage, roots in the bud stage and roots in a seedling stage; the ZmFAH1 gene can significantly improve the low temperature resistance of corn in the germination period, and also can enhance the low temperature resistance of corn in the germination period and the seedling period. The invention further provides a molecular marker DNCAPS708 which is located at the 708 -710 sites of the CDS region of the negative chain ZmFAH1 gene of the first chromosome of the corn, and three deleted basic groups are GAA / <->. The research finds that the overexpression of the ZmFAH1 gene can effectively improve the low temperature resistance of the corn, which is of great significance to the molecular biology mechanism research of the low temperature resistance function of the corn.

The invention discloses a corn germplasm resource viability cross-germplasm discrimination method, system, equipment and medium, and relates to the technical field of corn viability discrimination, in the method, hyperspectral data of an embryo area and hyperspectral data of an endosperm area are obtained; obtaining hyperspectral data of each sample corn seed; each piece of hyperspectral data is preprocessed; feature selection is carried out on each piece of preprocessed hyperspectral data, and a constructed double-branch spectrum Transform corn germplasm cross-germplasm viability detection model is trained in combination with viability label data of each sample corn seed; the trained double-branch spectrum Transform corn germplasm cross-germplasm viability detection model is utilized to discriminate the cross-germplasm corn to be discriminated, the viability prediction result of the cross-germplasm corn to be discriminated is obtained, and the accuracy and reliability of cross-germplasm corn germplasm resource viability discrimination can be remarkably improved.

The present invention provides: a drug which can improve the implantation rate of an embryo and can be used in conjunction with conventional drugs for infertility treatment; and a screening method and the like with which it is possible to reliably evaluate such a drug. Specifically, the integrin activator comprises: (1) a peptide composed of KFEERMRCKWMT, and (2) a peptide composed of KFEERMRCKWMT. (2) a peptide which is composed of KFEEERSRCKWMT; (3) a peptide comprising an amino acid sequence obtained by deleting, substituting and / or adding 1-3 amino acids in (1) or (2); or (4) a peptide obtained by binding any one of (1) to (3) to a membrane-permeable molecule. Provided are an artificial uterus and a screening method based on an implantation reproduction test system using a fertilized egg (for example, an embryo developed to an implanted blastocyst), the artificial uterus comprising: a hydrogel containing endometrial stromal cells and having an extracellular matrix as a main component; and a uterine epithelial organoid configured to be surrounded by the hydrogel.

The invention relates to the technical field of genetic engineering, in particular to a preparation method of a zebrafish trmt61a maternal zygote mutant, which comprises the following steps: co-injecting sgRNA and Cas9 protein of a targeted zebrafish trmt61a gene into a one-cell stage embryo of wild type zebrafish to obtain a trmt61a heterozygote mutant; the method comprises the following steps: constructing pGGDestISceIEG-4sgRNA plasmids, and injecting the pGGDestISceIEG-4sgRNA plasmids, an incision enzyme and a reaction buffer solution into a one-cell stage embryo of Tg (zpc: zcas9) transgenic zebrafish to obtain an adult zebrafish F0 generation; and hybridizing the female fish of the adult zebra fish F0 generation with the male fish of the trmt61a heterozygote mutant to obtain the zebra fish trmt61a maternal zygote mutant. The method breaks through the limitation of trmt61a gene homozygous mutation death, and realizes rapid preparation of the maternal zygote mutant.

The invention relates to the technical field of embryo culture platforms, in particular to a microfluidic-AI monitoring-metabolism regulation embryo culture platform and a preparation method thereof.The microfluidic-AI monitoring-metabolism regulation embryo culture platform comprises a microfluidic culture system used for achieving embryo culture, metabolite removal and culture medium replacement; the artificial intelligence monitoring system is used for remotely monitoring the embryonic development state in real time and predicting the embryonic development condition; the metabolism regulation and control system is used for feeding back and optimizing culture conditions online according to an artificial intelligence monitoring result and a metabolite analysis result; the intelligent control module is electrically connected with the micro-fluidic culture system, the artificial intelligence monitoring system and the metabolism regulation and control system, and the micro-fluidic culture system can accurately control the culture environment on the micrometer scale, including temperature, pH value and gas composition parameters. The dynamic fluid environment is closer to the in-vivo physiological environment of the embryo. The system can simulate periodic nutrient supply and metabolic waste removal in the uterus, and provides optimal growth conditions for embryos.

The invention discloses a corn ZmMYBR37 gene and an application of the corn ZmMYBR37 gene in regulating and controlling the size of corn kernels. The nucleotide sequence of the ZmMYBR37 gene is as shown in SEQ ID No: 1 in a sequence table. A mutant material with small grains and reduced hundred-grain weight is separated from a corn EMS mutant library, and a mutation site occurs on a function unknown gene ZmMYBR37, so that protein translation is terminated in advance. The information analysis finds that the gene coding protein has a conservative MYB structural domain, and the gene coding protein is speculated to belong to an MYB transcription factor family. Gene expression analysis finds that the gene is high in constitutive expression in corn and high in expression level in leaves and embryos. The corn material overexpressed with the ZmMYBR37 has the advantages that the grain size is increased, and the hundred-grain weight is obviously increased, so that the ZmMYBR37 has the potential of increasing the corn yield.

The invention belongs to the technical field of assisted reproduction, and particularly relates to a device and a method for gradually adjusting the glucose concentration of an in-vitro embryo culture solution. The device comprises at least two miniature liquid storage bins, wherein each miniature liquid storage bin is filled with a high-concentration glucose solution in advance; the slow release channel is respectively connected with each micro liquid storage bin and the culture solution main container; the culture solution main container is used for accommodating an embryo and a basal culture medium; the control unit is used for sequentially opening the release ports of the miniature liquid storage bins, so that the high-concentration glucose solution sequentially flows into the culture solution main container through the slow release channels. The technical means of staged closed-loop micro sugar control is introduced into the structure and the using method of the device, so that the accurate adjustment of the sugar concentration of the in-vitro embryo culture solution is realized, the requirements of embryos in different development stages can be effectively met, and the embryo culture quality and the subsequent development potential are remarkably improved.