18results about How to "Speed up genetic progress" patented technology

The invention relates to a molecular marker which is located at the 47023718th basic group from the 5tail end on a No.16 chromosome of a pig reference genome Sscrofa11.1 and is related to a pig internal farrowing litter weight standard deviation. The invention describes a primer pair and a kit for detecting the molecular marker, and application of the primer pair and the kit in identifying the size of the standard deviation of the litter weight in the pig, screening pig varieties or genetic breeding of the pig. The pig internal litter weight standard deviation of the AA genotype individual is 2.79 kg, the pig internal litter weight standard deviation of the GG genotype individual is 4.38 kg, and the AA genotype individual is obviously smaller than the GG type individual, namely, the pig resilience of the AA genotype individual is higher than that of the GG individual. The AA type individuals are selected, and the pigs with small standard deviation of litter weight and high restoring force in the pigs can be obtained. According to the method, the problem of determining the pig resilience in actual production is effectively solved, the breeding cost is reduced, the pig internal litter weight standard deviation is effectively reduced, so that the pig resilience is improved, the method is high in accuracy and low in detection cost, automatic detection can be realized, and the method has very high practical application value in the aspect of pig breeding.

The present invention belongs to the technical field of molecular genetics, and specifically relates to a SNP site related to the fine wool rate of fine wool sheep and its application. The SNP molecular marker described in the present invention is located at the A / T mutation of the 236304249th nucleotide site on chromosome 1 of the international sheep reference genome Oar_v4.0 version. The present invention also provides the use of a reagent for detecting the above-mentioned SNP site in detecting the fine wool rate trait of fine wool sheep or in molecular marker-assisted breeding of fine wool sheep, which has great application value in fine wool sheep variety screening, variety identification or molecular marker-assisted breeding; it is used for early breeding selection, shortening the generation interval, accelerating the breeding process, saving a lot of breeding costs, and has broad market prospects.

The application discloses a SNP molecular marker related to eye muscle area of a long white pig and application thereof, and relates to the technical field of biotechnology and molecular marker technology. The application adopts a SNP molecular marker as a detection target to determine or predict the size of eye muscle area of a long white pig. The sequence of a nucleic acid containing the SNP molecular marker is shown in SEQ ID No. 1. The SNP molecular marker is located at the 300th site from the 5' end in the nucleic acid sequence shown in SEQ ID No. 1, corresponds to the 10185432th site of the 6th chromosome of an international pig genome 11.1 version reference sequence, and the allele is C or T. The application provides an EMA related SNP molecular marker with clear location and clear effect direction, which is used for molecular determination and breeding application of the long white pig. Meanwhile, a corresponding selection and matching detection scheme is provided to improve the genetic level of a population EMA and accelerate the breeding process.

The invention discloses a polygene SNP (Single Nucleotide Polymorphism) molecular marker combination for predicting the first laying age of chickens and application of the polygene SNP molecular marker combination, and belongs to the fields of molecular genetics and agricultural animal breeding. The marker combination comprises SNP (Single Nucleotide Polymorphism) of four genes which influence gonad development and play a key expression regulation role in a reproduction endocrine network, and the SNP is specifically positioned on 273282348, 1169302032, 273273533 and 1169302490 of a reference genome GRCg7b. The invention proves that the specific genotype combination of the four SNP loci is obviously related to the early laying day age of the chicken. By simultaneously detecting the four SNP loci of an individual to be detected, the premature genetic potential of the individual can be calculated and evaluated. The multi-gene combined prediction system constructed by the invention overcomes the defect of insufficient prediction effectiveness of a single marker, and provides a reliable molecular tool for efficient and accurate breeding of precocious strains of laying hens.

The present invention relates to the field of molecular marker technology, and specifically provides a pig whole genome low-density 3K SNP chip and its application. The pig whole genome low-density 3K SNP chip provided by the present invention contains a SNP molecular marker combination for pig genotyping, and the SNP molecular marker combination consists of 3627 SNP molecular markers, and the position and polymorphism information of the SNP molecular markers are shown in Table 1 No. 1-3627. The pig whole genome low-density 3K SNP chip or molecular marker combination of the present invention can be applied to multiple fields such as pig whole genome breeding value evaluation, cluster analysis, molecular marker assisted breeding, etc., greatly reducing breeding costs.

The application provides a SNP molecular marker related to the egg production of chickens and an application thereof, and belongs to the technical field of molecular genetics and animal breeding. The SNP molecular marker is located at the 1141th site of a chicken genome ERalpha gene cDNA sequence, corresponds to the 136th bp in the sequence shown in SEQ ID NO. 1, and the base at the site is C or T. The SNP molecular marker can be used for early breeding selection of the egg production of chickens, effectively improves the average egg production of chicken groups, reduces breeding costs, and provides a convenient technical means for establishing a molecular marker assisted breeding of the egg production selection of chickens.

The invention relates to the technical field of biology and animal genetic breeding, and aims to provide a primer combination for detecting the genotype of a sheep Chr1: g.61541655 site, which is composed of the following three primers: a site A typing primer FAM with the sequence shown as SEQ ID NO: 1, a site B typing primer FAM with the sequence shown as SEQ ID NO: 2, and a site C typing primer FAM with the sequence shown as SEQ ID NO: 3. The sequence of the site T typing primer VIC is as shown in SEQ ID NO: 2; and the sequence of the universal primer is shown as SEQ ID NO: 3. T) of a sheep genome Chr1: g.61541655 site and the serum prolactin level are disclosed for the first time, it is clear that the AA genotype is a dominant genotype, and a reliable molecular target is provided for evaluating and screening sheep with high lactation potential from the genetic level.

The invention discloses application of an InDel molecular marker located on a pig chromosome 16 and related to nipple number heterosis, the locus of the InDel molecular marker is rs793470896, and the InDel molecular marker corresponds to GGAGGCACgt at the 74359589 bp position on the chromosome 16 of an international pig reference genome version 11.1; the gene type of the InDel molecular marker is GGAGGCAC / GGAGGCAC, GGAGGCAC / G or G / G. The gene type of the InDel molecular marker is GGAGGCAC / GGAGGCAC. The InDel molecular marker provided by the invention is used for marker-assisted selective breeding, pigs with the genotype of GGAGGCAC / GGAGGCAC in a group are gradually eliminated, and the nipple number of bred sows can be remarkably increased, so that the piglet breeding and lactation capabilities of the sows can be remarkably improved, the survival rate of piglets can be increased, and the production income can be increased.

The invention discloses a meat duck body length character molecular marker as well as an identification primer and application thereof. The molecular marker is a duck No.28 chromosome chr28: 301790 locus, the locus has a T / C allele, and the corresponding genotypes are TT, TC and CC. The invention further provides an allele specific primer combination based on the KASP technology, the allele specific primer combination comprises an FAM primer, a VIC primer and a universal primer, and the specific sequences of the FAM primer, the VIC primer and the universal primer are shown as SEQ ID NO.1-3. According to the method, marker-assisted selection is carried out on 10 commercial lines of Huaifu meat ducks, TT type individuals are preferentially selected and reserved, CC type individuals are eliminated, the semi-submersible length is increased by 3.95 cm, the body weight is increased by 200 g, and the progress rate of breeding generations is increased by 38% or above. The method is simple and convenient to operate, low in cost and high in accuracy, and a molecular tool and a technical support are provided for early-stage precise breeding of body length characters of Huaifu meat ducks and similar strains.

The invention discloses an efficient corn drought-resistant breeding method integrating molecular marker development and a whole genome prediction model. The efficient corn drought-resistant breeding method comprises the steps of hybridization matching and DH line induction, multi-environment phenotype and genotype analysis, three-level marker set screening, optimal prediction system construction, excellent selfing line screening and the like. The method has the remarkable effects that a whole genome selection technology is applied to identification and screening of the saline-alkali-tolerant and drought-tolerant corn materials, a whole genome breeding process for selecting the saline-alkali-tolerant and drought-tolerant materials is established, and generation intervals are shortened through early selection, so that the breeding value estimation accuracy is improved, the genetic progress is accelerated, and the breeding efficiency is improved. And the method has a good prediction effect on low-heritability and difficult-to-measure complex characters, and truly realizes the breeding practice guided by a genome technology.

The present invention relates to the technical field of laying hen breeding, and in particular to a SNP molecular module related to egg shell weight and its application. The SNP molecular module provided by the present invention avoids the problem that breeding with a single molecular marker cannot fully realize its production potential, can more accurately select laying hens with higher egg shell weight, and has a significant effect on regulating the egg shell weight of laying hens throughout the entire breeding cycle; it can help save production costs and accelerate genetic progress, has great economic application value and scientific research value, and is an optimization and upgrade of molecular marker technology.

The invention discloses a molecular marker related to duck sebum glyceride content traits and application, and belongs to the field of nucleic acid-containing determination methods. According to the technical scheme, the composition for detecting the polymorphism or genotype of the SNP-1 site in the duck genome is applied to identification or auxiliary identification of the content of the glyceride in the duck sebum, the SNP-1 site is an SNP site in the duck genome, the nucleotide type of the SNP-1 site is G or A, and the SNP-1 site is the 70th nucleotide in SEQ ID No.1; the composition comprises the PCR primer, and the PCR primer is composed of a single-chain DNA (deoxyribonucleic acid) with the nucleotide sequence of SEQ ID No.2 and a single-chain DNA with the nucleotide sequence of SEQ ID No.3. The SNP-1 can be used for early selection of duck sebum glyceride content traits, increasing or reducing the sebum glyceride content and accelerating genetic progress, is used for duck breeding, and has great economic value and scientific research value.

The application discloses a method for identifying or assisting in identifying the glycerol triester content of duck skin fat and related compositions and applications, and belongs to the field of nucleic acid determination methods. The method for identifying or assisting in identifying the glycerol triester content of duck skin fat comprises the following steps: detecting the genotype of a specific SNP site of a to-be-detected duck, and identifying or assisting in identifying the glycerol triester content of the duck skin fat of the to-be-detected duck according to the genotype of the specific SNP site of the to-be-detected duck. The specific SNP site is an SNP site in a duck genome, the nucleotide type of which is T or C, and is the 70th nucleotide in SEQ ID No. 1. The application can perform early selection on the glycerol triester content of duck skin fat, improve the duck breeding efficiency, and shorten the breeding cycle.

The present invention belongs to the technical field of molecular genetics, and specifically relates to a SNP site related to the fine wool rate of fine wool sheep and its application. The SNP molecular marker described in the present invention is located at the A / G mutation of the 60455332nd nucleotide site on chromosome 5 of the international sheep reference genome Oar_v4.0 version. The present invention also provides the use of a reagent for detecting the above-mentioned SNP site in detecting the fine wool rate trait of fine wool sheep or in molecular marker-assisted breeding of fine wool sheep, which has great application value in fine wool sheep variety screening, variety identification or molecular marker-assisted breeding; it is used for early breeding selection, shortening the generation interval, accelerating the breeding process, saving a lot of breeding costs, and has broad market prospects.

The present invention relates to the technical field of goat molecular marker screening, in particular to key genes JAK2 Insertion-deletion markers affecting goat growth traits and their applications. The insertion-deletion markers are located in goats JAK2 The second intron region of the gene, the nucleotide sequence is shown in SEQ ID NO.1, and there is a 5bp insertion or deletion of TCCAT at the 295bp from the 5' end. The results of association analysis show that the insertion and deletion marker is related to the chest depth, chest width, weight, body height, and chest circumference index of goats. The insertion and deletion marker can be used as a molecular genetic marker for goat growth traits, thereby accelerating the establishment of a goat population with excellent growth traits and improving the speed of breeding of fine varieties.

The application discloses application of a SNP molecular marker related to white dead fetus of pigs on chromosome 2 of pigs, wherein the site of the SNP molecular marker is rs345752533, and the genotype of the SNP molecular marker is TT, TA or AA. The SNP molecular marker provided by the application is significantly related to the white dead fetus trait of pigs, wherein the white dead fetus incidence of the pig with the SNP molecular marker genotype of TT or TA is significantly lower than that of the pig with the SNP molecular marker genotype of AA. Through breeding the pig with the SNP molecular marker genotype of TT or TA, the white dead fetus incidence can be reduced, and the reproduction efficiency of the sow can be improved.

The present invention belongs to the field of molecular biotechnology and molecular marker technology, and specifically relates to a SNP molecular marker and application thereof located on chromosome 5 of pigs and associated with the trait of piglets born alive. The SNP site of the SNP molecular marker located on chromosome 5 of pigs and associated with the trait of piglets born alive corresponds to the T>G mutation at the 89503311bp position on chromosome 5 of the international pig reference genome version 11.1. The molecular marker was detected by whole genome association analysis and found that the molecular marker significantly affects the trait of piglets born alive. The present invention also provides a primer pair and a kit for identifying the molecular marker. The molecular marker, primer pair or kit can be used to establish an efficient and accurate molecular marker-assisted breeding technology, which can be applied to the genetic improvement of the piglet number trait of piglets born alive, thereby increasing the piglet number born alive, increasing corporate profits, and increasing core competitiveness.

The present invention relates to the field of molecular marker technology, and specifically discloses a pig whole genome low-density 5K SNP chip and its application. The pig whole genome low-density 5K SNP chip provided by the present invention includes a SNP molecular marker combination for pig genotyping, and the SNP molecular marker combination consists of 5603 SNP molecular markers, and the SNP molecular markers are shown in Table 1. The SNP chip or molecular marker combination provided by the present invention can be applied to multiple fields such as pig whole genome breeding value evaluation, cluster analysis, molecular marker assisted breeding, etc., greatly reducing breeding costs.