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BMP6 gene as molecular marker of litter size trait of black goat

A technology of molecular markers and black goats, applied in the field of molecular biotechnology and molecular markers, to achieve huge profit potential, speed up the breeding process, and tap the effect of reproductive potential

Active Publication Date: 2018-09-14
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] And so far, no SNP molecular markers related to lambing traits have been found in the related regulatory region of BMP6 gene in the Chuanzhong black goat population

Method used

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  • BMP6 gene as molecular marker of litter size trait of black goat
  • BMP6 gene as molecular marker of litter size trait of black goat
  • BMP6 gene as molecular marker of litter size trait of black goat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035]In the embodiment, 530 Chuanzhong black goat ewes with complete lambing records were selected, and 2 mL of jugular vein blood was drawn and anticoagulated, and stored at -20°C for later use. The Chuanzhong black goats used in this study were all from the Nanjiang sheep breeding farm of Wenshi Agriculture and Animal Husbandry Co., Ltd. in Wuzhou City, Guangxi Province. The farm was raised in high-bed houses throughout the year, fed rations according to the uniform feeding standard, and had free access to food and water.

[0036] (1) The extraction of the blood genomic DNA of the black goat in central Sichuan was carried out with reference to the blood genomic DNA extraction kit (purchased from Tiangen Biochemical Technology Co., Ltd.), and detected by 1% agarose gel electrophoresis without dispersion or tailing phenomenon; An ultraviolet spectrophotometer was used to detect the quality and concentration of the extracted DNA. DNA samples with A260 / 280 ratio of 1.8 to 2.0 a...

Embodiment 2

[0048] (1) Amplification of the target fragment containing the SNP site significantly related to the lambing traits of Sichuan black goats: the target fragment is a 398bp nucleotide sequence on chromosome 23, and the upstream and downstream primers for sequence amplification are based on GenBank. The reported bovine BMP6 gene sequence (accession number: AC_000180.1), using Primer Premier 5.0 software to design primers for exon 3 of the BMP6 gene, amplified the partial sequence of the BMP6 gene of Chuanzhong black goat. The primers were synthesized by Huada Biotechnology Co., Ltd., and the specific sequences are as follows:

[0049] SEQ ID NO: 2

[0050] Upstream primer PCR-F: 5'-TGTGCCCTCACCCCTCTGTCTC-3';

[0051] SEQ ID NO: 3

[0052] Downstream primer PCR-R: 5'-CCCGGCCTTCCTCCTTTAACTC-3'.

[0053] (2) PCR amplification system and program setting

[0054] PCR reaction system: 10 μL system contains 1 μL of genomic DNA template, 0.2 μL of upstream and downstream primers, 5 μ...

Embodiment 3

[0062] Example 3 SNP site T951C effect analysis of molecular markers

[0063] The invention provides a SNP marker that can significantly increase the litter size of the Chuanzhong black goat, and using the SNP for marker-assisted selection can greatly increase the breeding process of the Chuanzhong black goat's reproductive performance.

[0064] In the present invention, the molecular marker SNP sites that affect the lamb size of sheep are all selected into CC type and CT type individuals, and then the average lamb size of each breeding ewe can be increased by 0.44 or 0.46, which shows that the reproductive performance is high. Breeding ewes is of great significance to reduce the cost of reproduction, improve the economic benefits of sheep farming and promote the rapid development of my country's mutton sheep industry. Among the individuals marked by this SNP, there is a significant difference in the litter size between the CC type and the CT type compared with the TT type indi...

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Abstract

The invention belongs to technical fields of molecular biology technology and molecular markers, and specifically relates to a BMP6 gene as a molecular marker of a litter size trait of a black goat. An SNP molecular marker site of the goat is as shown in SEQ ID NO.1, the 179th nucleic acid of the sequence fragment undergoes single base mutation and is named T951C. The SNP molecular marker is corresponding to T greater than C mutation at the 951bp (calculated with the first base of a coding zone sequence as +1 site) site in the coding zone sequence of the BMP6 gene (accession number: AC_000180.1) on No.23 chromosome of bovine released in GeneBank. Further analysis finds that the mutation fails to cause the coded amino acid sequence to change and represents synonymous mutation. A dominant allele genotype of the SNP molecular marker is selected, thereby accelerating genetic progress of the litter size trait of the goat, shortening breeding time of a reproductive trait of the goat, lowering reproduction cost, and increasing economic profits of meat goat breeding industry effectively.

Description

technical field [0001] The invention belongs to the field of molecular biotechnology and molecular marker technology, and in particular relates to the application of a goat SNP molecular marker in the study of goat lambing traits and goat breeding. Background technique [0002] Reproductive traits are important economic traits. For breeding stock, fecundity is productivity, and reproductive performance is directly related to the economic effect of mutton sheep industry. Reproductive performance mainly includes the total number of lambs born, the number of live lambs born, birth weight, newborn litter weight, weaned litter weight and other indicators. Studies have shown that the reproductive traits of sheep belong to the threshold traits of low heritability, and the low lambing rate is the main bottleneck restricting the development of high-quality and efficient sheep breeding. It is controlled by genes, and it is an additive-dominant gene action mode. In traditional breedin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 柳广斌杨新月孔令旋何玉强刘德武孙宝丽李耀坤
Owner SOUTH CHINA AGRI UNIV
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