Application of ncoa1 gene snp site and its kit

A kit and gene technology, applied to the application of NCOA1 gene SNP site and the field of detection kits

Active Publication Date: 2021-07-13
XINJIANG BIOTECH INST OF ACADEMY OF ANIMAL SCI CHINA & AUSTRALIA SHEEP BREEDING CENT OF XINJIANG ACADEMY OF ANIMAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no relevant literature report on whether the NCOA1 gene plays a role in regulating the reproductive performance of sheep

Method used

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  • Application of ncoa1 gene snp site and its kit
  • Application of ncoa1 gene snp site and its kit
  • Application of ncoa1 gene snp site and its kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The reagents used in the present invention are all commercially available products. Among them, PCR Master Mix was purchased from Beijing Tiangen Biotechnology Co., Ltd., and DNAMarker was purchased from BBI Company.

[0037] The primer pair SEQ ID NO: 1-2 and SEQ ID NO: 3-4 were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0038] (1) The present invention detects the genotype of the SNP site 32015148G>A at the NCOA1 gene by direct sequencing.

[0039] The forward primer is SEQ ID NO: 1: 5'-TTCCAGTGTTAGGTCATGCTA-3'

[0040] The reverse primer is SEQ ID NO:2: 5'—CACTCCCAATAAACAAAAGGT—3'

[0041] The target gene fragment was amplified using the primer sequences shown in SEQ ID NO.1-2. The PCR reaction system is: 10 μl of 2×PCRMaster Mix, 1 μl of PCR primer mix (10 μM, 0.5 μl of upstream primer and 0.5 μl of downstream primer), 1 μl of DNA sample to be tested ((concentration: 50 ng / μl), sterilized double distilled water to make up to 20 μ...

Embodiment 2

[0050] Example 2 Gene frequency, genotype frequency distribution and correlation analysis of SNP loci

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Abstract

The invention relates to the field of biotechnology, in particular to the application of the NCOA1 gene SNP site and a detection kit thereof. Through analysis, the present invention finds that the litter size of the Duolang sheep is closely related to the SNP sites at 32015148G>A and 32033237A>G of the NCOA1 gene. Based on this, the present invention provides a kit and detection method for predicting lamb size of sheep. The invention provides technical support at the molecular level for predicting and molecularly breeding sheep breeds with high lamb size, and the application of the invention will greatly increase the lamb size of sheep, produce better breeding economic benefits, and have good application prospects.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of the NCOA1 gene SNP site and a detection kit thereof. Background technique [0002] High fecundity is the basis for sheep to achieve high yield, and it is also one of the most important factors affecting the economic benefits of sheep breeding. There are about 700 kinds of sheep breeds in the world today. Most of the sheep are single-born sheep, but there are very few sheep breeds that can produce multiple lambs in one litter and can go into heat all the year round. This kind of sheep is commonly called multiple-born sheep, such as Australia's Booroola sheep, my country's small-tailed Han sheep and Hu sheep, and Xinjiang's Cele black sheep and Duolang sheep. Multiparous sheep have shown great advantages in the production of fat lambs and lambskin sheep. In terms of production efficiency and economic benefits, multiparous sheep are 2-3 times that of single-born sheep...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 史洪才牛志刚王珊冯东河李晓林汪立芹林嘉鹏吴阳升常璐黄俊成
Owner XINJIANG BIOTECH INST OF ACADEMY OF ANIMAL SCI CHINA & AUSTRALIA SHEEP BREEDING CENT OF XINJIANG ACADEMY OF ANIMAL SCI
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