181 results about "Versus gene" patented technology

The invention discloses a breast cancer recurrence risk prediction method and system based on multi-modal data missing interpolation and gene interpretability enhancement. The method comprises the following steps: firstly, dynamically generating and complementing features of a missing mode by matching a generative adversarial network with a mode missing mask matrix; then, a feature screening mechanism driven by gene information is introduced, through a multi-task learning network, image feature extraction is supervised by using a gene expression tag in a model training process, and image features highly associated with recurrence-related genes are screened out; and finally, fusing the complemented multi-modal time sequence characteristics by adopting Transform, and outputting a recurrence risk probability. According to the method, the robust prediction performance can be realized under the condition of data missing, and meanwhile, image interpretation with a molecular biology basis is provided for the feature screening process of the model, so that the reliability and clinical acceptability of the whole system are enhanced.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention relates to the technical field of biology, in particular to application of a biological product expressed by an EsPTI15 gene to regulation and control of tiller number. The nucleotide sequence of the EsPTI15 gene is shown as SEQ ID NO: 1, it is found for the first time that the tiller number of EsPTI15 gene overexpressed siberian wildrye is remarkably increased compared with wild siberian wildrye, and the result proves that overexpression of the EsPTI15 gene can positively regulate and control the plant tiller development process. The discovery not only enriches the molecular mechanism theory of plant tillering regulation, but also verifies that the EsPTI15 gene has the application potential of increasing the tillering number of the plant, and provides key theoretical support and gene resources for subsequent technical research and development of introducing the EsPTI15 gene through gene operation, increasing the tillering number of forage grass and further realizing biomass improvement.

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

The invention discloses the technical field of pathology and space transcriptomics, and particularly relates to a gene expression prediction method and system based on multi-modal comparative learning and a guidance mechanism. Cutting the histological slice image into image blocks according to space coordinates; according to the method, a local convolution branch and a global Transform branch are combined to extract image features, the image features are mapped to a shared potential space through projection, soft contrast, hard contrast and global consistency constraints are introduced into the space, and cross-modal alignment of an image modal and a gene expression modal is realized; an expression prediction head is introduced in the training stage, representation learning is directly guided by a regression signal, and the relation between feature learning and gene expression prediction is broken through; in the inference stage, k-nearest neighbor retrieval and a multi-distance weighted aggregation strategy are combined to infer a gene expression profile of an unknown position. According to the method, the accuracy and robustness of space gene expression prediction can be effectively improved, the tissue space heterogeneity structure is kept, and the method has high clinical application and scientific research and popularization value.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The invention discloses a combination of blocking agents for detecting drug resistance of mycobacterium tuberculosis. The combination comprises a first peptide nucleic acid blocking agent, a second peptide nucleic acid blocking agent, a third peptide nucleic acid blocking agent and a fourth peptide nucleic acid blocking agent. The first peptide nucleic acid blocker covers codons 511 and 513 of the rpoB gene. The second peptide nucleic acid blocker covers the codon 516 of the rpoB gene. And the third peptide nucleic acid blocking agent covers the codon 526 of the rpoB gene. And the fourth peptide nucleic acid blocking agent covers codons 531 and 533 of the rpoB gene. The respective sequences of the first peptide nucleic acid blocker, the second peptide nucleic acid blocker, the third peptide nucleic acid blocker, and the fourth peptide nucleic acid blocker match the wild type of the corresponding sequence of the rpoB gene. The invention also provides a kit and a method for detecting the drug resistance of mycobacterium tuberculosis.

The invention relates to the technical field of molecular detection, discloses a KASP molecular marker related to a sheep backfat thickness character, a detection primer group and application of the KASP molecular marker and the detection primer group, and aims to provide a KASP molecular marker for detecting a sheep Chr15: g.12672188 site Tgt; the invention discloses a specific primer group for C variation, which consists of the following three primers: a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 1, and a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 2, the nucleotide sequence of the locus C allele typing primer VIC is as shown in SEQ ID NO: 2. The invention discovers and discloses Tgt of Chr15: g.12672188 site in a sheep genome for the first time; the C single nucleotide polymorphism (SNP) is obviously associated with the backfat thickness character. Association analysis of 118 Auodurau lake rams proves that the back fat thickness of CC and TT genotype individuals is obviously higher than that of TC genotype individuals. The discovery reveals that the two homozygous genotypes of the site are unique genetic patterns of dominant genotypes, and a direct and reliable molecular target is provided for genetic improvement of the sheep backfat thickness.

Compositions and methods for detecting structural variations (SVs) in target genes or for genetic mapping of movable transposable elements are disclosed, the target genes relating to disease pathologies commonly found in large Mendelian genomics projects, and the movable transposable elements relating to genetic diseases, cancer, and aging. The method comprises: (i) contacting a sample containing genomic DNA with a DNA endonuclease for an effective amount of time to cleave the genomic DNA into fragments, the genomic DNA being uncrosslinked; (ii) subjecting the fragments obtained from step (b) to a DNA ligase to obtain circularized DNA; (iii) subjecting the circularized DNA to reverse PCR amplification containing a reverse primer, wherein the reverse primer is designed to match a expected wild-type sequence near a suspected mutant locus in the gene; and (iv) sequencing the amplified products. Exemplary conditions include Bardet-Biedel syndrome; severe upper and lower limb defects; retinitis pigmentosa; syndromic microcephaly; spastic paraplegia; and atypical hemolytic uremic syndrome.

The present invention is directed to improved materials and methods related to cytosine and adenosine base editing of genomic DNA.

This invention provides compositions and treatment methods for reducing hyperphenylalaninemia. [Solution] A genetically modified bacterium is provided, comprising: a) one or more genes encoding phenylalanine ammonia lyase (PAL), which are operably linked to a promoter that is not naturally associated with the PAL gene and can be directly or indirectly induced; b) one or more genes encoding a phenylalanine transporter, which are not naturally associated with the phenylalanine transporter gene and can be operably linked to a promoter that is directly or indirectly induceable; and c) one or more genes encoding mutant fumarate and nitrate reductase (FNR), which are not naturally associated with the FNR gene and can be operably linked to a promoter that is directly or indirectly induceable.

The present invention relates to siRNA and siRNA conjugates for inhibiting blood coagulation factor XII (FXII) gene expression, a pharmaceutical composition comprising the same, and uses thereof. Each nucleotide in the siRNA is independently modified or unmodified nucleotide, and the siRNA contains a positive-sense strand and an antisense strand. The siRNA as well as the conjugate and the pharmaceutical composition thereof can be used for effectively treating and / or preventing diseases related to FXII gene overexpression.

The application discloses a method and a kit for constructing a high-throughput multiplex fluorescence PCR-based HLA-CREG and HPA gene typing synchronous detection system. The application is based on common specific SNP sites, and two types of different primers are combined and designed to obtain a system for HLA cross-reactive group and HPA gene typing detection, which can realize rapid, simple, low-cost and high-throughput HLA epitope and HPA gene typing detection, so as to more effectively prevent platelet transfusion invalidity and immunological platelet transfusion invalidity.

The invention relates to the technical field of biology, and provides a guide editing system based on a bidirectional micro homologous arm and application of the guide editing system. Based on a guided editing technology, two pairs of independent pegRNAs are adopted, single-stranded DNA (ssDNA) micro homologous arms with the length of 20-60 nt are generated at the two ends of a genome target and the two ends of a donor carrier insertion fragment respectively, and efficient integration of donor DNA and genome DNA can be promoted. At the two target points of the genome, the ssDNA is homologous with the DNA sequence of the integration site in the donor vector, so that the seamless connection of the donor DNA and the genome DNA is effectively promoted by the micro homologous arm in the integration process. In the donor vector, ssDNA on the two sides of the integration part is homologous with a sequence on the upstream of a targeting nick site in a genome, so that the integration efficiency and accuracy are further improved. According to the method, high editing efficiency can be kept between different target sites and cell types, and large-fragment DNA can be inserted to realize seamless editing.

The application relates to a cancer driver gene explainable identification method based on trust calibration and prototype learning, and relates to the technical field of biological information identification. A gene graph is constructed by fusing a protein interaction network and gene multi-omics characteristics, and part of nodes are labeled. Label-aware message passing is performed through a trust calibration encoder, the neighborhood is split into a labeled part and a non-labeled part for independent calibration, and node embedding is adaptively fused. An angle margin prototype classifier is used to construct a class prototype on a hypersphere, the decision boundary is expanded, and a prediction result is output. A pivot node self-supervised regularizer is introduced, center nodes are screened from labeled driver genes, positive constraints are applied to neighbor non-labeled nodes, negative penalties are applied to non-neighbors, and a supervised boundary is maintained when non-labeled data is used. Finally, a structured explanation module is used to reuse the internal evidence of the model, a verifiable explanation is provided for prediction, and the unification of high precision and credible explanation is realized.

The invention belongs to the field of molecular biology and genetic engineering, and discloses a chemiluminescent reporter plasmid pBBR402 and application thereof in detection of the transcription level of a target gene of acetobacter pasteurianus. The plasmid comprises a replication element (oriV and rep) from a pBBR1MCS-2 plasmid, a kanamycin resistance gene from a pMS402 plasmid, a promoter insertion site (BamHI / AvaI / XhoI), a luxCDABE reporter gene cluster and a T1 transcription terminator, and the kanamycin resistance gene, the promoter insertion site (BamHI / AvaI / XhoI), the luxCDABE reporter gene cluster and the T1 transcription terminator are obtained from the pBBR1MCS-2 plasmid. The plasmid can be inserted into a to-be-detected gene promoter of acetobacter pasteurianus to drive luxCDABE expression. During application, the recombinant plasmid is transferred into acetobacter pasteurianus, the OD value and the LUM value of the living bacteria liquid are directly detected, and the transcription level is quantified by the LUM / OD value. The method is easy to operate, high in sensitivity and suitable for gene regulation and control research of acetobacter pasteurianus.

Provided are compositions and methods for reducing pre-existing antibodies against viral vector or gene-editing related proteins. The compositions showed reduction in various antibody titers by administration of a liposome composition complexed with a protein or fragment thereof of a viral vector. The liposomes comprise phosphatidylcholine and phosphatidylserine, wherein some or all of the PS is present as lyso-PS. The compositions and methods can be used in conjunction with gene therapy and nucleic acid based vector based vaccinations and therapeutics.

The invention discloses an HAPP gene and application thereof, and relates to the field of plant genetic engineering, the identified rice HAPP gene can increase the rice plant height and the seed size at the same time, and after the rice HAPP gene is fused with a PANDA gene, the rice plant height and the seed size can be remarkably increased, but the ear number is reduced. Meanwhile, analysis finds that HAPP originates from a Harbinger transposon Myb-like DNA binding protein, interacts with PANDA and is related to a gene expression mode, but has antagonism in function. Therefore, identification of the HAPP gene not only reveals a new function of the transposon-derived gene in regulation and control of rice yield traits, but also provides new theoretical support and practical guidance for rice breeding.

The present application relates to the technical field of CAR-T cell treatment, and particularly relates to a method for preparing universal CAR-T cells and application thereof. The VH domain and VL domain of the beta2M binding molecule provided in the present application contain CDR sequences, and can specifically bind to beta2M. The binding molecule is added to CAR-T cells, and the binding molecule can specifically bind to CAR-T cells without beta2M gene knockout, so as to separate CAR-T cells with beta2M gene knockout from CAR-T cells without beta2M gene knockout, and realize purification of universal CAR-T cells.

The utility model discloses a biological sample cracking device, a gene detection kit and gene detection equipment, relates to the field of biological sample treatment, and aims to solve the problem that the application scene of a transverse stirring structure is limited in related technologies. The biological sample cracking device is applied to the gene detection kit which is vertically used, a vertical cracking cavity is formed in the gene detection kit, a sample adding opening communicated with the cracking cavity is formed in the top end of the gene detection kit, and the biological sample cracking device comprises an opening sealing cover, a rotary transmission part and a stirring cutting part. The opening sealing cover is used for being connected with the sample adding opening and sealing the sample adding opening; one end of the rotary transmission part is rotatably connected with the opening sealing cover; the opening sealing cover is provided with a containing hole, and the transmission structure of the rotary transmission part is exposed through the containing hole; the stirring and cutting part is fixedly arranged at the other end of the rotary transmission part and is accommodated in the cracking cavity. The device has the advantages of being wide in application range, simple and compact in structure and capable of improving the cracking effect.

The invention relates to a method for improving the prokaryotic expression quantity of CRISPR-dCas9 (clustered regularly interspaced short palindromic repeats) protein. Specifically, the invention relates to a method for improving the expression level of dCas9 protein in a prokaryotic cell, and a leucine zipper gene sequence and a dCas9 gene sequence are connected and introduced into the prokaryotic cell. According to the method, the high-purity and high-concentration dCas9 protein can be obtained, the expression quantity of the dCas9 protein is remarkably higher than that of a common method using maltose binding protein (MBP) as a fusion molecular chaperone, the obtained dCas9-lzip fusion protein still has the capability of being normally combined with DNA in a targeted manner under the guidance of sgRNA, and the function of dCas9 is not influenced by connection of a lzip sequence.

The present application relates to a kind of HER2 gene and FGFR2 gene fluorescence in situ hybridization combined detection probe and its application, belong to biological medicine technical field.The present application provides a kind of probe and probe composition for tumor diagnosis and prognosis evaluation, including the first probe for detecting HER2 gene, and the second probe and the third probe for detecting FGFR2 gene;First probe, second probe and third probe are respectively marked with the first fluorochrome, second fluorochrome and third fluorochrome that can produce different colors;By the present application, a kind of fluorescence in situ hybridization combined detection method and its kit for simultaneously detecting HER2 and FGFR2 gene variation are provided;Wide application range can be used in various tissue and cell samples needing simultaneously detecting HER2 and FGFR2 gene state, one detection, two results, meet the goal of clinical test quality improvement and efficiency improvement;It has quite extensive application prospect.

This invention belongs to the interdisciplinary field of bioscience and information technology, and relates to a method for assessing the intensity of gene transcriptional regulation based on multi-omics data. Addressing the challenges of systematically integrating multi-omics data and quantitatively characterizing the correspondence between regulatory elements and genes, as well as the intensity of regulation, in existing transcriptional regulation analyses, this invention integrates epigenomics, transcriptomics, and three-dimensional genomics data to construct a site-gene regulatory intensity model. This method extracts candidate cis-regulatory sites and calculates their regulatory activities. It then determines the site-gene regulatory weights by combining distance weighting and three-dimensional genomic contact information, thereby obtaining the regulatory intensity at the site-gene pair and gene levels. An iterative algorithm distinguishes between positive and negative regulatory effects, achieving correction and summarization of regulatory intensity. This invention can quantitatively assess the regulatory intensity of cis-regulatory elements on target genes and the overall regulatory effect on genes, and is suitable for studying gene transcriptional regulation mechanisms and analyzing gene expression changes under different treatment conditions.

The invention relates to a method and a kit for detecting SMA related gene copy number based on an NGS technology. The detection method comprises the following steps: extracting DNA of a sample to be detected and constructing an NGS library; designing a specific capture probe based on SMN1 / SMN2 differential sites a, b and c, and performing whole exon sequencing to obtain original sequencing data; performing data preprocessing on the sequencing original data; selecting a human control gene set as a housekeeper gene candidate set, and screening n genes close to SMN1 and SMN2 whole exon sequencing depth from the housekeeper gene candidate set as housekeeper genes k; standardizing the sequencing depth of each housekeeping gene k, and normalizing to obtain a theta value; the average value of all housekeeping genes theta is recorded as a correction factor value theta, and the copy states of the SMN1 and SMN2 genes in the DNA sample to be detected are corrected. The method has the beneficial effects that the quantitative accuracy of the copy number of the SMN1 and SMN2 genes is improved, and meanwhile, the detection efficiency is improved.

The invention relates to the field of animal husbandry molecular breeding and precise breeding, in particular to an animal husbandry improved variety breeding twin-fetal cattle system based on gene screening. The system comprises an ovulation potential typing module, a uterine bearing capacity quantification module, a sex compatibility verification module and a decision module. Constructing a combined evaluation model of the double-ovulation basis and the uterus metabolism capability by systematically integrating site specific amplification and gene expression quantification technologies; the core of the method is that a precise reproduction instruction is generated according to full-link data of genotypes and phenotypes; according to the method, a single ovulation rate screening mode is changed, the problem of abortion and weak abortion caused by insufficient intrauterine environment is effectively solved, and the success rate of double-fetal pregnancy is remarkably increased.

The present disclosure provides a method for performing phenotypic fit analysis. The method comprises computer processing an input dataset to produce a set of genes and a set of gene-phenotype associations (GPAs) associated with the set of genes. The method further comprises determining, for a subject, a plurality of subject-gene similarity subscores, based at least in part on the GPAs associated with the set of genes. The method further comprises determining a predicted likelihood of association between the subject and at least a subset of the set of genes, based at least in part on the plurality of subject-gene similarity subscores.

The invention discloses a group of nocardia seriolae mycolic acid synthase genes and a construction and application method of a deletion strain of the nocardia seriolae mycolic acid synthase genes, and belongs to the technical field of disease prevention and control. Comprising the following steps: strain culture and whole genome extraction, specific primer design and gene fragment cloning, deletion plasmid construction, competent cell preparation, electrotransformation and deletion strain screening, and deletion strain characteristic verification. By analyzing the synergistic function of nocardia seriola seriola mycolic acid synthesis key genes, the core effect of the nocardia seriola seriola mycolic acid synthesis key genes in a synthesis pathway is clear to fill the blank of multi-gene association research, and a core theoretical basis is provided for revealing the pathogenic mechanism of nocardia seriola seriola mycolic acid.

The application discloses a method for improving cellulase activity by using a combination of an endogenous promoter and an associated 5' UTR of Trichoderma reesei and an engineering strain constructed by the method. TrAE The 5' UTR upstream of the TrAE gene TrAE Overexpressing a transcriptional regulator xyr1 The expression cassette of the gene is transformed into Trichoderma reesei Δ ku70 Rut‑C30 The application also discloses a high-yield cellulase Trichoderma reesei strain QEaeuX, and a method for producing high-activity cellulase by using the strain. ku70 Rut‑C30 The cellulase activity produced by the strain in a 96 h shake flask culture is 2.6 IU / mL, which is 101% higher than that of the original strain Δ The application has a good industrial development and application prospect.

The invention discloses an application of a corn ERF transcription factor ZmERF75 in regulation and control of corn stalk development and cell wall composition. Researches find that by reducing the expression of the ZmERF75 gene (such as a zmerf75 mutant), the plant height, the stem diameter, the stem bending force and the leaf tension of the corn can be obviously reduced, and vascular bundles are sparsely distributed. Meanwhile, the stem cellulose content of the zmerf75 mutant is reduced, the lignin content is increased, the monosaccharide content is changed, and the saccharification efficiency is remarkably improved. An EMSA and yeast single hybridization experiment proves that the ZmERF75 protein can be specifically combined with DNA in a CUI gene promoter region. The invention discloses a key regulation and control effect of ZmERF75 in corn stalk development and cell wall synthesis, and provides a new gene target and theoretical basis for improving the biomass utilization efficiency of corn stalks, optimizing cell wall composition and improving stalk characters through a molecular breeding means.