570 results about "Versus gene" patented technology

The invention belongs to the technical field of gene sequencing, and particularly relates to a method for efficiently identifying an exogenous DNA insertion site in a genome by using a high-throughput sequencing technology. The method comprises the following steps: (a) extracting a sample, building a database and sequencing; (b) data quality control; (c) comparing data; (d) filtering comparison results; (e) preliminary screening; and (f) screening and identifying the positions of the exogenous insertion elements. According to the method, the high-throughput sequencing data and the genome and exogenous fragment sequences are compared and analyzed to obtain the candidate positions of the insertion sites, so that the test efficiency can be remarkably improved, manual judgment and screening work can be effectively replaced, and the result accuracy can be improved.

The invention relates to the field of biotechnology and plant breeding, and particularly provides a method for fixing rice heterosis by using a DMP gene. Aiming at the problems of low maturing rate and complex operation of the existing apomixis system, the haploid induction functions of the rice OsDMP8 and OsDMP13 genes are verified for the first time, the OsPAIR1, OsREC8, OsOSD1 and OsDMP genes are synchronously knocked out through a CRISPR / Cas9 technology, a'MiMe-OsDMP 'four-gene editing system is constructed, and a new thought is provided for construction of the rice apomixis system. A new solution is provided for fixing heterosis of rice apomixis, the efficiency and quality of agricultural production can be greatly improved, and the method has high economic value and wide application prospects.

The invention discloses a method for displaying foreign protein by utilizing salmonella and application of the method, and belongs to the technical field of biological medicine. According to the invention, the SpyCatcher and an outer membrane protein Lpp-OmpA gene are fused and are expressed in salmonella, a recombinant strain is constructed, and the SpyCatcher is displayed on the surface of bacteria; the foreign protein gene and the SpyTag gene are fused and subjected to prokaryotic expression purification, and recombinant foreign protein is obtained; after the recombinant foreign protein and the recombinant salmonella are incubated, the foreign protein can be anchored on the surface of the salmonella through a SpyCatcher-SpyTag covalent bond. When the foreign protein is an antigen protein, the foreign protein is displayed on the surface of the attenuated bacterial vector, and a live vector vaccine can be prepared. The invention provides a novel universal antigen display method, overcomes the limitation of the traditional antigen display method on the protein size and conformation, and has a good application prospect in live vector vaccine construction.

The invention discloses a method for identifying, quantifying and visualizing an integron-drug-resistant gene cassette in an environmental sample. The method comprises the following steps: performing high-throughput identification, quantification and genome visualization on a movable genetic element integron based on three-generation sequencing and a drug-resistant gene mediated by the movable genetic element integron; comprising the following steps: 1) integron identification and quantification: constructing an integrase gene database by applying a'collection-screening-verification 'strategy and applying the strategy to integron identification and quantification; 2) identifying the drug-resistant gene cassette and visualizing the genome, namely obtaining DNA (Deoxyribose Nucleic Acid) by utilizing an optimized variable region gene cassette primer in combination with a PCR (Polymerase Chain Reaction) technology, sequencing by adopting PacBio three-generation full-length amplicons to obtain a long sequence of diversity of the gene cassette, and identifying the type of the drug-resistant gene cassette mediated by the integrator on the basis of a professional database; and carrying out visual representation on the integron-multi-drug-resistant gene cassette array by using a self-defined R script, and carrying out quantification based on short sequence and drug-resistant gene cassette long fragment mapping. The invention provides technical support for monitoring and controlling the development of bacterial drug resistance.

The invention relates to the technical field of bioinformatics, in particular to a method for identifying RNA (Ribonucleic Acid) from a transposon element based on RNA sequencing data. According to the invention, the exon level of the teRNA is taken as an analysis object, compared with a teRNA site, the teRNA exon not only realizes more accurate site identification, but also retains the existing form of TE in a transcription product, including whether a splicing site and a polyadenylic acid signal are provided or not, whether the TE is chimeric with a gene or other TE sites or not and the like. And compared with the level of the teRNA transcript, as a constituent part of the transcript, the teRNA exon significantly reduces the sequence assembly difficulty of the short-read-length sequencing data. According to the method, the Bayesian model, the parametric assembly algorithm and the local site de novo assembly algorithm are combined, and through mutual correction of different assembly algorithms and repeated comparison sequencing reading length before and after assembly, the assembly false positive is reduced, so that the teRNA recognition accuracy is improved.

The invention discloses a breast cancer recurrence risk prediction method and system based on multi-modal data missing interpolation and gene interpretability enhancement. The method comprises the following steps: firstly, dynamically generating and complementing features of a missing mode by matching a generative adversarial network with a mode missing mask matrix; then, a feature screening mechanism driven by gene information is introduced, through a multi-task learning network, image feature extraction is supervised by using a gene expression tag in a model training process, and image features highly associated with recurrence-related genes are screened out; and finally, fusing the complemented multi-modal time sequence characteristics by adopting Transform, and outputting a recurrence risk probability. According to the method, the robust prediction performance can be realized under the condition of data missing, and meanwhile, image interpretation with a molecular biology basis is provided for the feature screening process of the model, so that the reliability and clinical acceptability of the whole system are enhanced.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention relates to the technical field of biology, in particular to application of a biological product expressed by an EsPTI15 gene to regulation and control of tiller number. The nucleotide sequence of the EsPTI15 gene is shown as SEQ ID NO: 1, it is found for the first time that the tiller number of EsPTI15 gene overexpressed siberian wildrye is remarkably increased compared with wild siberian wildrye, and the result proves that overexpression of the EsPTI15 gene can positively regulate and control the plant tiller development process. The discovery not only enriches the molecular mechanism theory of plant tillering regulation, but also verifies that the EsPTI15 gene has the application potential of increasing the tillering number of the plant, and provides key theoretical support and gene resources for subsequent technical research and development of introducing the EsPTI15 gene through gene operation, increasing the tillering number of forage grass and further realizing biomass improvement.

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention provides a multi-scale causal discovery method and system based on collective behavior modeling, and relates to the technical field of causal discovery, and the method comprises the steps: obtaining single-cell RNA sequencing data, the single-cell RNA sequencing data being a gene expression matrix of a plurality of cells; single-cell RNA sequencing data is input into a multi-scale causal model for reasoning, a multi-scale causal diagram is obtained, and the multi-scale causal diagram is used for describing the multi-scale causal relationship between genes and between genes and gene programs; wherein the multi-scale causal model takes a single gene as an individual variable, through collective behavior modeling, the single gene is aggregated into a collective variable representing a gene program, and a multi-scale collaborative learning strategy is adopted to collaboratively learn the multi-scale causal relationship between individuals and between individuals and collective. According to the method, a collective behavior modeling framework is innovatively constructed, key collective behaviors driving state changes can be effectively identified, and the causal discovery accuracy, rationality and robustness are improved.

The invention discloses a novel gene editing element self-cleaning recombinant vector suitable for tobacco and application thereof.The recombinant vector comprises an expression cassette of a germ cell specific expression Barnase gene, the expression cassette comprises a germ cell specific expression promoter DLL, a lethal gene Barnase and an NOS terminator, the recombinant vector is obtained by integrating the expression cassette into a tobacco editing vector, and the expression cassette is a recombinant vector with a specific expression function. A linked recombinant vector is formed with other gene editing elements, and the sequence of an expression cassette is as shown in SEQ ID No.1. After a plant cell is transformed by the gene editing recombinant vector, the gene editing element of a T0-generation plant is not influenced to play a role, Barnase protein capable of killing female and male gametes is expressed during sexual propagation, most of T0-generation seeds do not contain CRISPR editing elements, filial generation plants do not contain CRISPR editing elements any more, the efficiency of obtaining the CRISPR-free editing element is improved, and the method is suitable for large-scale popularization and application. And manpower, material resources and financial resources are greatly saved.

The invention discloses a binuclear internuclear allele expression analysis method and device and a storage medium, and relates to the technical field of bioinformatics transcriptome analysis of binuclear fungi. The method comprises the following steps: respectively carrying out comparative analysis on sequencing data of binuclear fungi and two nuclear reference genomes; performing kernel source classification on each read in the sequencing data by using an SVM model; taking all gene sequences of the kernel 1 as a query set, taking a gene sequence of the kernel 2 as a target set, and constructing a similarity score matrix among the gene sequences by using a BLAST tool; combining the similarity score with gene annotation information, and analyzing and establishing an internuclear allele relationship through a LightGBM model; and generating a counts file, performing FPKM standardization processing, and analyzing the standardized expression quantity in combination with the internuclear allele relationship index to obtain an analysis result. According to the invention, the analysis of the interkaryotic alleles of the binuclear fungi is realized.

The invention belongs to the technical field of aquaculture biological breeding, and particularly relates to a KASP marker of a vibrio harveyi resistant gene jam2a of cynoglossus semilaevis and application of the KASP marker. The KASP markers are respectively positioned at 14: 17 279470, 14: 17 281640 and 14: 17 283306 sites of the 14 # chromosome of the cynoglossus semilaevis, the polymorphism of the sites is T / C, G / T and T / A, and when the genotypes of the three sites are respectively homozygous TT, homozygous GG and homozygous TT, the tested individual of the cynoglossus semilaevis is a disease-resistant individual. The KASP marker significantly associated with the jam2a gene provided by the invention can be used for molecular marker-assisted breeding of the vibriosis resistance character of the cynoglossus semilaevis, and provides important technical support for improving the breeding efficiency of disease resistance of the cynoglossus semilaevis.

Provided herein is a recombinant AAV (rAAV) comprising an AAV capsid and a vector genome packaged therein, wherein the vector genome comprises an AAV 5' inverted terminal repeat (ITR), an expression cassette comprising engineered nucleic acid sequence encoding human Tafazzin and operably linked thereto regulatory sequences, and an AAV 3' ITR. Also provided is a pharmaceutical composition comprising a rAAV as described herein in a formulation buffer, and a method of treatment of Barth Syndrome or a disease associated with a mutation in a Tafazzin (TAZ) gene.

The invention discloses the technical field of pathology and space transcriptomics, and particularly relates to a gene expression prediction method and system based on multi-modal comparative learning and a guidance mechanism. Cutting the histological slice image into image blocks according to space coordinates; according to the method, a local convolution branch and a global Transform branch are combined to extract image features, the image features are mapped to a shared potential space through projection, soft contrast, hard contrast and global consistency constraints are introduced into the space, and cross-modal alignment of an image modal and a gene expression modal is realized; an expression prediction head is introduced in the training stage, representation learning is directly guided by a regression signal, and the relation between feature learning and gene expression prediction is broken through; in the inference stage, k-nearest neighbor retrieval and a multi-distance weighted aggregation strategy are combined to infer a gene expression profile of an unknown position. According to the method, the accuracy and robustness of space gene expression prediction can be effectively improved, the tissue space heterogeneity structure is kept, and the method has high clinical application and scientific research and popularization value.

Provided is an siRNA for inhibiting the gene expression of complement factor B (CFB), or a pharmaceutical composition or conjugate containing the siRNA. The siRNA contains a sense strand and an antisense strand. Each nucleotide in the siRNA is an independent modified or unmodified nucleotide. The sense strand contains a segment of nucleotide sequence I, and the nucleotide sequence I and a nucleotide sequence show in SEQ ID NO: 1 have an equal length and differ by no more than 3 bases. The antisense strand contains a nucleotide sequence II, and the nucleotide sequence II and a nucleotide sequence shown in SEQ ID NO: 2 have an equal length and differ by no more than 3 bases. The siRNA or the pharmaceutical composition or conjugate containing the siRNA can effectively treat and / or prevent diseases related to the mRNA level of the gene expression of the CFB.

The invention provides an expression data preprocessing method, device and system and a storage medium, and relates to the technical field of space-time omics. The expression data preprocessing method comprises the following steps: obtaining sequencing data based on a space-time group technology, and obtaining a gene expression matrix according to the sequencing data; carrying out dimension reduction processing to obtain a cell population; determining a differential gene list among the cell populations, and constructing a gene set expression matrix; and converting the gene set expression matrix into contribution degrees, and constructing a gene contribution degree matrix. According to the method, cell grouping and gene screening technologies are applied, a gene set expression matrix is accurately converted, matrix dimensionality reduction is remarkably achieved, similar feature gene sets are more aggregated, some potential valuable features are reserved as far as possible, the analysis accuracy is improved, the use efficiency of computing resources is optimized, and the method is suitable for large-scale popularization and application. And a more reliable and efficient research way is provided for scientific researchers.

The invention relates to the technical field of data processing, and provides a genome prediction method, system and equipment, and the method comprises the steps: constructing a dynamic weight proportion distribution mechanism, and obtaining a genome weight proportion according to the dynamic weight proportion distribution mechanism; a TRM-CNN prediction model is constructed; obtaining a target genome coding vector of the preprocessed target genome data; performing feature extraction on the target genome coding vector to obtain a first target genome feature and a second target genome feature; fusing the first target genome feature and the second target genome feature to obtain a target genome fusion feature vector; and transmitting the target genome fusion feature vector to a regression layer, and obtaining a prediction result of a phenotype corresponding to the genome. According to the method, two neural network architectures of Transform and CNN are combined, local and global sample information features can be balanced, and the accuracy and generalization ability of genome prediction are remarkably improved.

The invention provides double-stranded oligonucleotide for inhibiting LPA gene expression and application of the double-stranded oligonucleotide, and relates to the technical field of nucleic acid drugs. The double-stranded oligonucleotide comprises a positive-sense strand and an antisense strand, wherein the positive-sense strand comprises a nucleotide sequence which is the same as or basically the same as at least 15 continuous nucleotides in the sequence as shown in SEQ ID NO.1, and the antisense strand comprises at least 17 nucleotides and is complementary or basically complementary with the positive-sense strand to form a duplex region; each nucleotide of the double-stranded oligonucleotide is independently selected from an unmodified or modified nucleotide. The double-stranded oligonucleotide can inhibit LPA gene expression and can treat siRNA drugs of diseases related to LPA gene expression, such as cardiovascular and cerebrovascular diseases.

The present invention relates to the technical field of nucleic acid drugs. Disclosed are a double-stranded oligonucleotide inhibiting LPA gene expression and the use thereof. The double-stranded oligonucleotide comprises a sense strand and an antisense strand, wherein the sense strand contains a nucleotide sequence that is identical or substantially identical to at least 15 consecutive nucleotides in the sequence as shown in SEQ ID NO. 1, and the antisense strand contains at least 17 nucleotides and is complementary or substantially complementary to the sense strand to form a duplex region. Each nucleotide of the double-stranded oligonucleotide is independently selected from unmodified or modified nucleotides. The double-stranded oligonucleotide can inhibit LPA gene expression and solve the problem of an siRNA drug for treating LPA gene expression related diseases such as cardiovascular and cerebrovascular diseases.

The present disclosure discloses a method for using plant heterosis. The method comprises the following steps: S1, transforming the meiosis of germ cells of hybrids into mitosis-like so as to obtain gametes whose genotype and chromosome ploidy are consistent with hybrids by using gene mutation or gene engineering technology; and S2, influencing and involving in the development of gametes or embryos in plants by using gene mutation and gene engineering technology, wherein a protein involved is MTL protein.

The invention discloses an antigen polypeptide related to BRAF gene V600E mutation and application of the antigen polypeptide. On the basis of a classical tumor neoantigen affinity prediction technology, an advanced immunogenicity prediction AI algorithm is adopted to improve the accuracy of tumor neoantigen prediction; the antigen polypeptide related to BRAF gene V600E mutation is characterized in that a replacement peptide for enhancing immunogenicity is further excavated based on a biological information screening technology of amino acid editing, the sequence of the antigen polypeptide related to BRAF gene V600E mutation is shown as any one of SEQ ID NO: 1-3, and the antigen polypeptide can remarkably activate T cells specifically aiming at BRAF mutation of a human body and enhance the killing ability of the T cells to BRAF mutation tumor cells. Therefore, the antigen polypeptide obtained by the invention has good potential of polypeptide vaccines and good clinical transformation and disease prevention prospects.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The invention belongs to the technical field of genes, and particularly relates to an amplification primer group of a renal cyst disease gene PKD1, a mutation detection method and application, the amplification primer group comprises four pairs of primers for specifically amplifying first to 34 exons of the PKD1 gene; the mutation detection method comprises the following steps: acquiring genome DNA (Deoxyribose Nucleic Acid); carrying out ultra-long fragment PCR amplification on the genome DNA through the primers, carrying out electrophoresis detection, and purifying a target fragment; mixing the purified amplification product with genome DNA in proportion, constructing a sequencing library, and performing high-throughput sequencing through target area capture; and analyzing sequencing data, and screening pathogenic PKD1 gene mutation. Compared with the prior art, the PKD1 ultra-long fragment PCR amplification primer and the renal cyst disease gene PKD1 mutation detection method have the advantages that true and false genes of PKD1 can be distinguished, the diagnosis accuracy is greatly improved, the operation process is simplified, the timeliness is improved, and the detection flux is improved.

The present invention relates to an siRNA for inhibiting FXI gene expression, an siRNA conjugate, a pharmaceutical composition containing same, and the use thereof. Each nucleotide in the siRNA is independently a modified or unmodified nucleotide, and the siRNA contains a sense strand and an antisense strand. The siRNA and the conjugate and pharmaceutical composition thereof can effectively treat and / or prevent diseases associated with FXI gene overexpression.

The invention discloses a lutjanus erythropterus breeding auxiliary method and system based on multi-character collaborative selection, and the method comprises the steps: carrying out phenotype data collection and gene detection, and constructing a standardized phenotype matrix and a genotype matrix; performing genetic parameter estimation and breeding value prediction based on the standardized phenotype matrix and the genotype matrix to obtain breeding value prediction information; the method comprises the following steps: analyzing an association relationship between characters of lutjanus rubripes to be bred, constructing a character association network, and carrying out multi-character dynamic weight configuration to obtain a dynamic weight set; performing genome selection index calculation and parent priority analysis according to the breeding value prediction information and the dynamic weight set to generate a parent selection priority list; and generating a candidate parent pool through the parent selection priority list, performing hybridization combination analysis, performing genetic gain prediction on the generated hybridization combination, and generating a hybridization auxiliary scheme. The genetic gain accuracy and balance of breeding of the lutjanus erythropterus are improved, the limitation of traditional experience selection and matching is broken through, and the breeding efficiency is improved.

The invention discloses a combination of blocking agents for detecting drug resistance of mycobacterium tuberculosis. The combination comprises a first peptide nucleic acid blocking agent, a second peptide nucleic acid blocking agent, a third peptide nucleic acid blocking agent and a fourth peptide nucleic acid blocking agent. The first peptide nucleic acid blocker covers codons 511 and 513 of the rpoB gene. The second peptide nucleic acid blocker covers the codon 516 of the rpoB gene. And the third peptide nucleic acid blocking agent covers the codon 526 of the rpoB gene. And the fourth peptide nucleic acid blocking agent covers codons 531 and 533 of the rpoB gene. The respective sequences of the first peptide nucleic acid blocker, the second peptide nucleic acid blocker, the third peptide nucleic acid blocker, and the fourth peptide nucleic acid blocker match the wild type of the corresponding sequence of the rpoB gene. The invention also provides a kit and a method for detecting the drug resistance of mycobacterium tuberculosis.

The invention discloses branched fluorine-containing poly (beta-amino ester) as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The branched fluorine-containing poly (beta-amino ester) is prepared by polymerizing a diacrylate monomer, a triacrylate monomer, micromolecular organic amine and a fluorine-containing alkyl chain monomer through a Michael addition reaction and then blocking with an end-capping reagent. The preparation method is simple, and the process is controllable. The polymer has controllable molecular weight, branching degree and fluorine content, can be self-assembled with a gene drug to form a stable nano-composite, can mediate efficient gene transfection, has good in-vitro and in-vivo gene editing ability, and can be used for treating genetic metabolic diseases and the like.

The invention relates to the technical field of molecular detection, discloses a KASP molecular marker related to a sheep backfat thickness character, a detection primer group and application of the KASP molecular marker and the detection primer group, and aims to provide a KASP molecular marker for detecting a sheep Chr15: g.12672188 site Tgt; the invention discloses a specific primer group for C variation, which consists of the following three primers: a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 1, and a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 2, the nucleotide sequence of the locus C allele typing primer VIC is as shown in SEQ ID NO: 2. The invention discovers and discloses Tgt of Chr15: g.12672188 site in a sheep genome for the first time; the C single nucleotide polymorphism (SNP) is obviously associated with the backfat thickness character. Association analysis of 118 Auodurau lake rams proves that the back fat thickness of CC and TT genotype individuals is obviously higher than that of TC genotype individuals. The discovery reveals that the two homozygous genotypes of the site are unique genetic patterns of dominant genotypes, and a direct and reliable molecular target is provided for genetic improvement of the sheep backfat thickness.