358 results about "Versus gene" patented technology

The invention relates to the field of biotechnology and plant breeding, and particularly provides a method for fixing rice heterosis by using a DMP gene. Aiming at the problems of low maturing rate and complex operation of the existing apomixis system, the haploid induction functions of the rice OsDMP8 and OsDMP13 genes are verified for the first time, the OsPAIR1, OsREC8, OsOSD1 and OsDMP genes are synchronously knocked out through a CRISPR / Cas9 technology, a'MiMe-OsDMP 'four-gene editing system is constructed, and a new thought is provided for construction of the rice apomixis system. A new solution is provided for fixing heterosis of rice apomixis, the efficiency and quality of agricultural production can be greatly improved, and the method has high economic value and wide application prospects.

The invention relates to the technical field of bioinformatics, in particular to a method for identifying RNA (Ribonucleic Acid) from a transposon element based on RNA sequencing data. According to the invention, the exon level of the teRNA is taken as an analysis object, compared with a teRNA site, the teRNA exon not only realizes more accurate site identification, but also retains the existing form of TE in a transcription product, including whether a splicing site and a polyadenylic acid signal are provided or not, whether the TE is chimeric with a gene or other TE sites or not and the like. And compared with the level of the teRNA transcript, as a constituent part of the transcript, the teRNA exon significantly reduces the sequence assembly difficulty of the short-read-length sequencing data. According to the method, the Bayesian model, the parametric assembly algorithm and the local site de novo assembly algorithm are combined, and through mutual correction of different assembly algorithms and repeated comparison sequencing reading length before and after assembly, the assembly false positive is reduced, so that the teRNA recognition accuracy is improved.

The invention discloses a breast cancer recurrence risk prediction method and system based on multi-modal data missing interpolation and gene interpretability enhancement. The method comprises the following steps: firstly, dynamically generating and complementing features of a missing mode by matching a generative adversarial network with a mode missing mask matrix; then, a feature screening mechanism driven by gene information is introduced, through a multi-task learning network, image feature extraction is supervised by using a gene expression tag in a model training process, and image features highly associated with recurrence-related genes are screened out; and finally, fusing the complemented multi-modal time sequence characteristics by adopting Transform, and outputting a recurrence risk probability. According to the method, the robust prediction performance can be realized under the condition of data missing, and meanwhile, image interpretation with a molecular biology basis is provided for the feature screening process of the model, so that the reliability and clinical acceptability of the whole system are enhanced.

The invention belongs to the technical field of molecular biology and genetic engineering, and particularly relates to a passion fruit PeDREB1 gene and application thereof in regulating temperature and drought stress tolerance. By overexpressing the passion fruit PeDREB1 transcription factor in the plant, the tolerance of the transgenic plant to temperature stress (low temperature and high temperature) and drought stress can be obviously enhanced, the growth recovery capability of the transgenic arabidopsis thaliana overexpressed with PeDREB1 after low temperature stress is obviously superior to that of a wild type, and the leaf area growth rate is increased by about 0.45-2.28%; under the condition that 0.2 mol / L to 0.3 mol / L mannitol simulates drought stress, the seed germination rate is increased by 5.39 percent to 9.29 percent, and the root length growth rate is increased by 1.08 percent to 6.72 percent; the invention provides a clear gene target and a directly applicable molecular resource for plant stress resistance molecular breeding, can provide theoretical support for subsequent clarification of a regulation mechanism of plant response temperature and drought stress, and also lays a foundation for plant stress resistance genetic improvement and new germplasm creation.

The invention relates to the technical field of biology, in particular to application of a biological product expressed by an EsPTI15 gene to regulation and control of tiller number. The nucleotide sequence of the EsPTI15 gene is shown as SEQ ID NO: 1, it is found for the first time that the tiller number of EsPTI15 gene overexpressed siberian wildrye is remarkably increased compared with wild siberian wildrye, and the result proves that overexpression of the EsPTI15 gene can positively regulate and control the plant tiller development process. The discovery not only enriches the molecular mechanism theory of plant tillering regulation, but also verifies that the EsPTI15 gene has the application potential of increasing the tillering number of the plant, and provides key theoretical support and gene resources for subsequent technical research and development of introducing the EsPTI15 gene through gene operation, increasing the tillering number of forage grass and further realizing biomass improvement.

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

The present invention relates to the fields of biotechnology and plant breeding, specifically providing a method for fixing heterosis in rice using the DMP gene. To address the low fruit set rate and complex operation issues of existing apomictic reproduction systems, the present invention verifies for the first time the haploid-inducing function of the rice genes OsDMP8 and OsDMP13. Furthermore, the CRISPR / Cas9 technology is used to simultaneously knock out the OsPAIR1, OsREC8, OsOSD1, and OsDMP genes, constructing a "MiMe-OsDMP" four-gene editing system. This system provides new ideas for constructing apomictic reproduction systems in rice and offers a new solution for fixing heterosis in rice apomictic reproduction. This system can significantly improve the efficiency and quality of agricultural production, and has significant economic value and broad application prospects.

The invention provides a multi-scale causal discovery method and system based on collective behavior modeling, and relates to the technical field of causal discovery, and the method comprises the steps: obtaining single-cell RNA sequencing data, the single-cell RNA sequencing data being a gene expression matrix of a plurality of cells; single-cell RNA sequencing data is input into a multi-scale causal model for reasoning, a multi-scale causal diagram is obtained, and the multi-scale causal diagram is used for describing the multi-scale causal relationship between genes and between genes and gene programs; wherein the multi-scale causal model takes a single gene as an individual variable, through collective behavior modeling, the single gene is aggregated into a collective variable representing a gene program, and a multi-scale collaborative learning strategy is adopted to collaboratively learn the multi-scale causal relationship between individuals and between individuals and collective. According to the method, a collective behavior modeling framework is innovatively constructed, key collective behaviors driving state changes can be effectively identified, and the causal discovery accuracy, rationality and robustness are improved.

The invention belongs to the technical field of aquaculture biological breeding, and particularly relates to a KASP marker of a vibrio harveyi resistant gene jam2a of cynoglossus semilaevis and application of the KASP marker. The KASP markers are respectively positioned at 14: 17 279470, 14: 17 281640 and 14: 17 283306 sites of the 14 # chromosome of the cynoglossus semilaevis, the polymorphism of the sites is T / C, G / T and T / A, and when the genotypes of the three sites are respectively homozygous TT, homozygous GG and homozygous TT, the tested individual of the cynoglossus semilaevis is a disease-resistant individual. The KASP marker significantly associated with the jam2a gene provided by the invention can be used for molecular marker-assisted breeding of the vibriosis resistance character of the cynoglossus semilaevis, and provides important technical support for improving the breeding efficiency of disease resistance of the cynoglossus semilaevis.

The invention discloses the technical field of pathology and space transcriptomics, and particularly relates to a gene expression prediction method and system based on multi-modal comparative learning and a guidance mechanism. Cutting the histological slice image into image blocks according to space coordinates; according to the method, a local convolution branch and a global Transform branch are combined to extract image features, the image features are mapped to a shared potential space through projection, soft contrast, hard contrast and global consistency constraints are introduced into the space, and cross-modal alignment of an image modal and a gene expression modal is realized; an expression prediction head is introduced in the training stage, representation learning is directly guided by a regression signal, and the relation between feature learning and gene expression prediction is broken through; in the inference stage, k-nearest neighbor retrieval and a multi-distance weighted aggregation strategy are combined to infer a gene expression profile of an unknown position. According to the method, the accuracy and robustness of space gene expression prediction can be effectively improved, the tissue space heterogeneity structure is kept, and the method has high clinical application and scientific research and popularization value.

Provided is an siRNA for inhibiting the gene expression of complement factor B (CFB), or a pharmaceutical composition or conjugate containing the siRNA. The siRNA contains a sense strand and an antisense strand. Each nucleotide in the siRNA is an independent modified or unmodified nucleotide. The sense strand contains a segment of nucleotide sequence I, and the nucleotide sequence I and a nucleotide sequence show in SEQ ID NO: 1 have an equal length and differ by no more than 3 bases. The antisense strand contains a nucleotide sequence II, and the nucleotide sequence II and a nucleotide sequence shown in SEQ ID NO: 2 have an equal length and differ by no more than 3 bases. The siRNA or the pharmaceutical composition or conjugate containing the siRNA can effectively treat and / or prevent diseases related to the mRNA level of the gene expression of the CFB.

The invention discloses an antigen polypeptide related to BRAF gene V600E mutation and application of the antigen polypeptide. On the basis of a classical tumor neoantigen affinity prediction technology, an advanced immunogenicity prediction AI algorithm is adopted to improve the accuracy of tumor neoantigen prediction; the antigen polypeptide related to BRAF gene V600E mutation is characterized in that a replacement peptide for enhancing immunogenicity is further excavated based on a biological information screening technology of amino acid editing, the sequence of the antigen polypeptide related to BRAF gene V600E mutation is shown as any one of SEQ ID NO: 1-3, and the antigen polypeptide can remarkably activate T cells specifically aiming at BRAF mutation of a human body and enhance the killing ability of the T cells to BRAF mutation tumor cells. Therefore, the antigen polypeptide obtained by the invention has good potential of polypeptide vaccines and good clinical transformation and disease prevention prospects.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The invention belongs to the technical field of genes, and particularly relates to an amplification primer group of a renal cyst disease gene PKD1, a mutation detection method and application, the amplification primer group comprises four pairs of primers for specifically amplifying first to 34 exons of the PKD1 gene; the mutation detection method comprises the following steps: acquiring genome DNA (Deoxyribose Nucleic Acid); carrying out ultra-long fragment PCR amplification on the genome DNA through the primers, carrying out electrophoresis detection, and purifying a target fragment; mixing the purified amplification product with genome DNA in proportion, constructing a sequencing library, and performing high-throughput sequencing through target area capture; and analyzing sequencing data, and screening pathogenic PKD1 gene mutation. Compared with the prior art, the PKD1 ultra-long fragment PCR amplification primer and the renal cyst disease gene PKD1 mutation detection method have the advantages that true and false genes of PKD1 can be distinguished, the diagnosis accuracy is greatly improved, the operation process is simplified, the timeliness is improved, and the detection flux is improved.

The present invention relates to an siRNA for inhibiting FXI gene expression, an siRNA conjugate, a pharmaceutical composition containing same, and the use thereof. Each nucleotide in the siRNA is independently a modified or unmodified nucleotide, and the siRNA contains a sense strand and an antisense strand. The siRNA and the conjugate and pharmaceutical composition thereof can effectively treat and / or prevent diseases associated with FXI gene overexpression.

The invention discloses a lutjanus erythropterus breeding auxiliary method and system based on multi-character collaborative selection, and the method comprises the steps: carrying out phenotype data collection and gene detection, and constructing a standardized phenotype matrix and a genotype matrix; performing genetic parameter estimation and breeding value prediction based on the standardized phenotype matrix and the genotype matrix to obtain breeding value prediction information; the method comprises the following steps: analyzing an association relationship between characters of lutjanus rubripes to be bred, constructing a character association network, and carrying out multi-character dynamic weight configuration to obtain a dynamic weight set; performing genome selection index calculation and parent priority analysis according to the breeding value prediction information and the dynamic weight set to generate a parent selection priority list; and generating a candidate parent pool through the parent selection priority list, performing hybridization combination analysis, performing genetic gain prediction on the generated hybridization combination, and generating a hybridization auxiliary scheme. The genetic gain accuracy and balance of breeding of the lutjanus erythropterus are improved, the limitation of traditional experience selection and matching is broken through, and the breeding efficiency is improved.

The invention discloses a combination of blocking agents for detecting drug resistance of mycobacterium tuberculosis. The combination comprises a first peptide nucleic acid blocking agent, a second peptide nucleic acid blocking agent, a third peptide nucleic acid blocking agent and a fourth peptide nucleic acid blocking agent. The first peptide nucleic acid blocker covers codons 511 and 513 of the rpoB gene. The second peptide nucleic acid blocker covers the codon 516 of the rpoB gene. And the third peptide nucleic acid blocking agent covers the codon 526 of the rpoB gene. And the fourth peptide nucleic acid blocking agent covers codons 531 and 533 of the rpoB gene. The respective sequences of the first peptide nucleic acid blocker, the second peptide nucleic acid blocker, the third peptide nucleic acid blocker, and the fourth peptide nucleic acid blocker match the wild type of the corresponding sequence of the rpoB gene. The invention also provides a kit and a method for detecting the drug resistance of mycobacterium tuberculosis.

The invention relates to the technical field of molecular detection, discloses a KASP molecular marker related to a sheep backfat thickness character, a detection primer group and application of the KASP molecular marker and the detection primer group, and aims to provide a KASP molecular marker for detecting a sheep Chr15: g.12672188 site Tgt; the invention discloses a specific primer group for C variation, which consists of the following three primers: a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 1, and a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 2, the nucleotide sequence of the locus C allele typing primer VIC is as shown in SEQ ID NO: 2. The invention discovers and discloses Tgt of Chr15: g.12672188 site in a sheep genome for the first time; the C single nucleotide polymorphism (SNP) is obviously associated with the backfat thickness character. Association analysis of 118 Auodurau lake rams proves that the back fat thickness of CC and TT genotype individuals is obviously higher than that of TC genotype individuals. The discovery reveals that the two homozygous genotypes of the site are unique genetic patterns of dominant genotypes, and a direct and reliable molecular target is provided for genetic improvement of the sheep backfat thickness.

The invention discloses an organizational structure division method for spatial transcriptome data, which comprises the following steps of: firstly, performing coordinate calibration, hypervariable gene screening and gene expression standardization preprocessing on original spatial transcriptome data, and constructing a standardized data set containing a spatial adjacency relation and a standardized gene expression profile; an initial hyperedge is generated based on a k-nearest neighbor algorithm, cross-domain noise is dynamically eliminated in combination with a hyperedge decomposition algorithm guided by gene expression, and a hypergraph structure with double constraints of spatial proximity and gene expression homogeneity is formed; designing an auto-encoder architecture comprising a hypergraph attention layer, compressing high-dimensional data to a low-dimensional potential space, reconstructing a loss optimization architecture by using gene expression, and generating low-dimensional representation with topology retentivity and function consistency; and finally, organizational structure division is carried out on the low-dimensional representation clustering based on a Gaussian mixture model. According to the method, the spatial continuity of organization structure division is remarkably improved, and a semantic gap of cross-resolution data is effectively bridged.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIC1 gene and a GmNIC2 gene to regulation and control of soybean cyst nematode resistance of plants, and the nucleotide sequence of the GmNIC1 gene is shown as SEQ ID NO.1; the nucleotide sequence of the GmNIC2 gene is as shown in SEQ ID NO. 2. The invention discloses the negative regulation effect of GmNIC1 and GmNIC2 genes in the resistance of soybean to cyst nematode (SCN) for the first time, and by constructing gene silencing mutants of GmNIC1 and GmNIC2, it is verified that the resistance of soybean to SCN can be remarkably enhanced due to function deficiency of the gene family. The application provides a new target for deep understanding of a soybean-nematode interaction mechanism, and provides a brand new gene resource and technical path for molecular design breeding of resistant soybeans.

Methods of predicting a chromatin state metric associated with a genomic region in a sample are provided. The methods comprise: receiving sequence data comprising genetic data and epigenetic data indicative of the presence of one or more epigenetic bases including methylated cytosine and hydroxymethylated cytosine at one or more genomic positions, the one or more epigenetic bases, the genetic data and epigenetic data obtained from a single assay; and predicting for each base or set of bases of the genomic region and using the sequence data associated with the genomic region, a value of the chromatin state metric, wherein the predicting is performed using a machine learning model.

The invention discloses an application of interaction of a pear transcription factor PbrDDI2 and a PbrAPX14 gene promoter in regulation and control of maturation and senescence of pear fruits. The nucleotide sequence of the pear transcription factor PbrDDI2 is as shown in SEQ ID NO.1 in a sequence table, and the amino acid sequence of the gene coded protein is as shown in SEQ ID NO.2. The invention discloses the transcription factor PbrDDI2 for regulating and controlling the PbrAPX14 gene expression of the pear fruit for the first time. The invention clarifies an action mechanism of interaction of cis-acting elements (TTTGTCG / CGACAAA) in PbrDDI2 and PbrAPX14 gene promoters in clearing endogenous H2O2 accumulation and further inhibiting synthesis of ethylene so as to inhibit fruit ripening and senescence, and provides a theoretical and practical basis for cultivating anti-senescence high-quality pear varieties.

The invention discloses a stropharia rugoso-annulata SRU6 gene promoter, a silent vector containing the promoter and application of silent stropharia rugoso-annulata endogenous genes, and relates to the field of molecular biology and gene engineering. According to the principle of shRNA mediated gene silencing, a silencing vector suitable for gene silencing of stropharia rugoso-annulata is constructed, and meanwhile, a stropharia rugoso-annulata efficient genetic transformation system is established; by utilizing the silent vector and the transformation system established by the invention, the expression quantity of the target gene can be reduced by about 80%, the purpose of gene function research is achieved, and the silent vector and the transformation system have very strong theoretical research and application values.

An siRNA capable of inhibiting the expression of apolipoprotein C3 (APOC3) gene, comprising a sense strand and an antisense strand, wherein the sense strand and the antisense strand are respectively composed of nucleotide sequence I or nucleotide sequence II comprising 19 modified or unmodified nucleotides, and the nucleotide sequence I and the nucleotide sequence II are at least partially reverse complementary to form a double-stranded region, and the nucleotide sequence II is at least partially reverse complementary to a nucleotide sequence segment in the mRNA of APOC3 gene expression; in the direction from the 5'-end to the 3'-end, at least one of the 3-6th nucleotides of the nucleotide sequence II is a stabilized modified nucleotide. The siRNA as described above, as well as the pharmaceutical composition and siRNA conjugate comprising the siRNA, can effectively treat and / or prevent diseases or disorders related to APOC3 gene expression, and have a significantly reduced off-target effect.

This invention discloses a method for directed evolution of antibiotic-resistant Escherichia coli strains based on cytidine deaminase, belonging to the field of microbial directed evolution and genetic engineering technology. This method uses E. coli as the host, introducing a recombinant plasmid expressing an optimized double-stranded cytidine deaminase mutant. Utilizing the low toxicity and high mutagenicity of this mutant, continuous passage evolution is carried out under gradient concentrations of aminoglycoside antibiotics (kanamycin and streptomycin). Combined with whole-genome sequencing, molecular docking, and reverse genetics verification, the A145T missense mutation in the wcaE gene is identified as the core functional site. This invention overcomes the shortcomings of traditional spontaneous and chemical mutagenesis, which suffer from low efficiency and significant strain damage. The mutation type is controllable, the evolutionary cycle is short, and the obtained engineered strains can tolerate up to 300 mg / L kanamycin while exhibiting streptomycin cross-resistance, and the genetic stability of the tolerance trait is strong. This method is simple, highly reproducible, and suitable for industrial breeding of stress-resistant E. coli, and can be widely applied in antibiotic fermentation, industrial microbial culture, and other scenarios.

Compositions and methods for detecting structural variations (SVs) in target genes or for genetic mapping of movable transposable elements are disclosed, the target genes relating to disease pathologies commonly found in large Mendelian genomics projects, and the movable transposable elements relating to genetic diseases, cancer, and aging. The method comprises: (i) contacting a sample containing genomic DNA with a DNA endonuclease for an effective amount of time to cleave the genomic DNA into fragments, the genomic DNA being uncrosslinked; (ii) subjecting the fragments obtained from step (b) to a DNA ligase to obtain circularized DNA; (iii) subjecting the circularized DNA to reverse PCR amplification containing a reverse primer, wherein the reverse primer is designed to match a expected wild-type sequence near a suspected mutant locus in the gene; and (iv) sequencing the amplified products. Exemplary conditions include Bardet-Biedel syndrome; severe upper and lower limb defects; retinitis pigmentosa; syndromic microcephaly; spastic paraplegia; and atypical hemolytic uremic syndrome.

The application belongs to the field of genetic engineering and genetic breeding, and particularly relates to a high-temperature response gene HSP70 expression regulation SNP marker of Crassostrea gigas and application of the SNP marker in identifying high-temperature resistant oyster individuals. The SNP marker is the 251th base in the sequence shown in SEQ ID NO: 2, and the mutation type is C / G (trans-regulation site Marker13973). The application provides a SNP marker which is significantly related to the expression of the HSP70 gene, and the advantage of the application is that the genotype of parent oysters can be identified before seed breeding, and the tolerance of offspring to high-temperature environment is improved. The SNP marker obtained in the research has high reliability and stable results.

The invention provides a capsicum annuum cell nucleus male fertility gene Msc-4, a co-separation molecular marker of the gene and application of the capsicum annuum cell nucleus male fertility gene Msc-4. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID NO. 1. The invention further provides a molecular marker SNP-31372133 co-separated with the gene Msc-4, and the molecular marker SNP-31372133 can assist in screening of a hot pepper material with nuclear male sterility. The invention provides a new nucleus sterile germplasm resource and a new nucleus male sterility gene resource for the research on the nucleus male sterility of the pepper, and plays an important role in the application in the field of pepper seed production and breeding.

The present invention relates to the field of biological genetic engineering, and particularly to an SNP molecular marker related to the IREB2 gene and its application. The present invention provides an SNP molecular marker related to the IREB2 gene, and the molecular marker includes a first molecular marker and a second molecular marker; the nucleotide sequence of the first molecular marker is as shown in SEQ ID NO.1, and there is an A / G mutation at the 74th bp of this sequence; the nucleotide sequence of the second molecular marker is as shown in SEQ ID NO.2, and there is an A / T mutation at the 102nd bp of this sequence. The SNP molecular marker of the present invention can accurately distinguish NDCAMA patients, carriers and normal people, and the time required for the identification result is short and the price is low.

The present invention is directed to improved materials and methods related to cytosine and adenosine base editing of genomic DNA.