Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular markers co-separated with bruchid resistance genes VrPGIP of vigna radiate and application of molecular markers

A technology of molecular markers and anti-weevil, which is applied in the fields of genetic engineering, molecular biology and genetic breeding, can solve the problems of weak research at the molecular level and lagging research on mung bean weevil, and achieve the effect of accurate cost

Inactive Publication Date: 2016-09-21
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
View PDF5 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on mung bean at home and abroad is still quite lagging behind, and the research at the molecular level is even weaker.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular markers co-separated with bruchid resistance genes VrPGIP of vigna radiate and application of molecular markers
  • Molecular markers co-separated with bruchid resistance genes VrPGIP of vigna radiate and application of molecular markers
  • Molecular markers co-separated with bruchid resistance genes VrPGIP of vigna radiate and application of molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 TC1966 / Zhonglv No. 1 F1 and F2 population construction and phenotypic identification

[0033] Using TC1966 as the male parent and Zhonglu No. 1 as the female parent, we prepared hybrids and constructed a segregation population of TC1966 / Zhonglu 1 containing 150 individuals. Each F2 individual The corresponding TC1966 / Zhonglv 1 F2:3 family was obtained by selfing, and the insect resistance was identified. The specific method is as follows:

[0034] 30 healthy seeds were randomly selected for each material, including 2 copies of Gandouxiang Zhonglu No. 1 and Kangdouxiang TC1966 control, placed in a plastic box in the Kangdouxiang identification room, the temperature was kept between 25°C and 29°C, and the humidity was low. Control it between 70%-80%. Put 400-500 mung bean weevil adults that have just emerged for 1-3 days in each plastic box, let it lay eggs at random, and remove the adults when the amount of eggs dropped by each seed is more than 5. After 40-...

Embodiment 2

[0038] Example 2 Development of SSR Molecular Marker Linked to Mung Bean Anti-Elephant Gene VrPGIP

[0039] Acquisition of mung bean resistance gene VrPGIP: polymorphic SSR markers were screened out by using the F2 segregation population crossed by the resistant parent TC1966 and the sensitive parent Zhonglu 1 and the resistant and sensitive pools. The anti-weevil gene was preliminarily located on Chromosome No. 5 of mung bean. According to the mung bean genome sequence published by NCBI (GenBank accession number: JJMO00000000), primers were designed with the software Primer3.0, and a total of 3143 pairs of SSR primers were generated on chromosome 5 of mung bean. The DNA of mung bean leaves was extracted, and the success rate of primers was tested. The results showed that a total of 1254 pairs of SSR primers detected clear bands at 100-300 bp, indicating that the success rate of primer design was high.

Embodiment 3

[0040] Example 3 Fine Mapping of Mung Bean Anti-Elephant Gene VrPGIP

[0041] The DNA of each strain of the parent and F2 population was extracted by the improved CTAB method, the concentration was measured by a UV spectrophotometer, and then diluted to 25 ng / μL, and stored in a -20°C refrigerator for later use.

[0042] Referring to the method of Chen et al. (2015), the mung bean genomic DNA was used as a template for PCR reaction. 10μl PCR reaction system includes: 40ng DNA, 1×Taq enzyme buffer (10mmolL -1 Tris-HCl, pH8.8; 10mmol L -1 KCl; 10 mmol L -1 (NH 4 ) 2 SO 4 ; 1.5mmolL -1 MgCl 2 ; 0.1% Triton X-100), 1 mmol L -1 dNTPs, 0.25 μmol L each for upstream and downstream primers -1 and 1U Taq DNA polymerase, ddH 2 O to make up to 10 μl. Reaction program: pre-denaturation at 95°C for 5min; denaturation at 95°C for 30s, annealing at 55°C for 45s, extension at 72°C for 45s, 32-35 cycles; final extension at 72°C for 5min. The whole reaction was carried out on Dongsh...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides molecular markers co-separated with bruchid resistance genes VrPGIP of vigna radiate. The bruchid resistance genes VrPGIP of the vigna radiate are positioned in regions of 5.558Mb and 5.625Mb of a chromosome 5 of the vigna radiate; the molecular markers co-separated with the genes VrPGIP comprise SSR (Simple Sequence Repeat) markers Vr05-5590 and Vr05-5597; physical distances between the Vr05-5590 and the Vr05-5597 and the bruchid resistance genes VrPGIP of the vigna radiate are 1.5kb and 850bp respectively. The invention also provides application of the molecular markers in mapping of the bruchid resistance genes of the vigna radiate or genetic breeding of the vigna radiate. According to the molecular markers and the application thereof disclosed by the invention, by finely mapping the bruchid resistance genes VrPGIP of the vigna radiate, two SSR markers co-separated with the bruchid resistance genes VrPGIP of the vigna radiate are found; the development of the molecular markers provides a feasible way for assistant breeding of bruchid resistant molecules of the vigna radiate.

Description

technical field [0001] The invention relates to the technical fields of genetic engineering, molecular biology and genetic breeding, in particular to a molecular marker co-separated with mung bean resistance gene VrPGIP and its application. Background technique [0002] Mung bean (Vigna radiata) is a plant of the genus Vigna radiata in the family Fabaceae and Papilionaceae. China is one of the birthplaces of mung bean and has a wide variety of mung bean varieties. China, Myanmar and other countries are the main mung bean exporters. Because of its short growth period, wide adaptability, and good nitrogen fixation ability, it is an indispensable food crop and an important economic crop for rational allocation of planting resources, crop rotation, intercropping, disaster reduction and relief; at the same time, mung beans are rich in protein. , vitamins, a variety of minerals and essential amino acids for the human body. It also has the effects of clearing heat and detoxificati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/686C12Q1/6895C12Q2600/13C12Q2600/156C12Q2525/151
Inventor 陈红霖程须珍王丽侠王素华
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com