98 results about "Vigna" patented technology

The invention provides molecular markers co-separated with bruchid resistance genes VrPGIP of vigna radiate. The bruchid resistance genes VrPGIP of the vigna radiate are positioned in regions of 5.558Mb and 5.625Mb of a chromosome 5 of the vigna radiate; the molecular markers co-separated with the genes VrPGIP comprise SSR (Simple Sequence Repeat) markers Vr05-5590 and Vr05-5597; physical distances between the Vr05-5590 and the Vr05-5597 and the bruchid resistance genes VrPGIP of the vigna radiate are 1.5kb and 850bp respectively. The invention also provides application of the molecular markers in mapping of the bruchid resistance genes of the vigna radiate or genetic breeding of the vigna radiate. According to the molecular markers and the application thereof disclosed by the invention, by finely mapping the bruchid resistance genes VrPGIP of the vigna radiate, two SSR markers co-separated with the bruchid resistance genes VrPGIP of the vigna radiate are found; the development of the molecular markers provides a feasible way for assistant breeding of bruchid resistant molecules of the vigna radiate.

The invention belongs to the field of disease resistance detection of horticultural crops and relates to a seedling-stage root rot resistance identification method for vigna unguiculata. The method comprises the steps of carrying out germination hastening on vigna unguiculata seeds, carrying out sowing and seedling raising, carrying out special strain inoculating so as to carry out screening, preparing a vigna unguiculata root rot bacterium spore suspension, carrying out pathogenic bacterium infestation, carrying out onset examination and the like. Root systems of young seedlings are soaked and infested by adopting a bacterial solution so as to subject the vigna unguiculata young seedlings to artificial root rot bacterium infection; and the vigna unguiculata seeds are cultured in seedling raising matrixes until each young seedling has two leaves and one heart, and then, the young seedlings are drawn out for infestation, so that the growth vigor and size of the vigna unguiculata young seedlings are easily controlled, the vigna unguiculata young seedlings are easily drawn out, and the consistency and reliability of identification results are guaranteed. Special indicator strains are obtained through screening, the infesting effect is better by adopting appropriate time of infestation and appropriate root rot bacterium spore suspension concentration, the bacterium inoculating is uniform, and the onset is rapid. According to the method, by only injuring roots of different plants of consistent growth vigor through directly and naturally rubbing the roots and then infesting the roots with the indicator strains, the root rot resistance of a large amount of vigna unguiculata breeding materials can be identified simply, conveniently, rapidly and reliably, so that the efficiency of variety resistance identification is increased.

The invention discloses a preparation method of a canned red Vigna angularis. The method includes: firstly conducting exhaust and softening treatment on a mixture of red Vigna angularis and soup; and then carrying out cooking and stewing treatment in order, thus obtaining the product. The canned red Vigna angularis prepared by the method provided by the invention maintains the original flavor and grain shape of red Vigna angularis, adopts Vigna angularis, white granulated sugar and pure water as raw materials, and does not contain other added substances. The grain shape and flavor of red Vigna angularis are perfectly kept, nutrients are not lost, the quality safety level of Vigna angularis processed products is improved, and the consumption space of Vigna angularis is expanded.

The invention discloses a vigna sesquipedalis color-protecting and fresh-keeping method including the following steps: choosing vigna sesquipedalis pods which are harvested in a sunny day after 10-15 days after flowering and have no mechanical damage and no diseases and insect pests; soaking the vigna sesquipedalis pods in a vigna sesquipedalis fresh-keeping solution for 0.5-10 min, taking out, after the vigna sesquipedalis fresh-keeping solution on the fruit surface is air-dried, packing with fresh-keeping bags, and storing, wherein the vigna sesquipedalis fresh-keeping solution is an apple polyphenol aqueous solution with the mass fraction of 0.05-5%. After the vigna sesquipedalis pods are treated by the vigna sesquipedalis color-protecting and fresh-keeping method, the vigna sesquipedalis commodity rate can be improved by 15-69%.

The invention discloses a sow feed containing Chinese herbal medicines. The sow feed containing the Chinese herbal medicine is mainly prepared from the following raw materials through pulverizing and even mixing in parts by weight: 50-60 parts of corn flour, 40-50 parts of cottonseed meal, 20-25 parts of peanut meal, 20-24 parts of bran, 10-12 parts of oyster powder, 4-6 parts of tortoise jelly powder, 6-8 parts of soy protein powder, 4-6 parts of Herba Plantaginis, 1-2 parts of groundsel, 20-24 parts of sweet potato dry powder, 10-12 parts of Vigna sinensis powder, 1-3 parts of salt, 15-16 parts of soya-bean oil, 1-3 parts of folic acid, 1.5-2 parts of baking soda, 15-20 parts of lactic acid, 1-1.2 parts of choline chloride, 0.8-1.2 parts of yeast, 2-4 parts of decavitamin, and 10-12 parts of a Chinese medicine additive. The sow feed is reasonable in proportion, rich in nutrition and good in palatability, and comprises various Chinese herbal medicines, the meat quality of animals is improved, disease resistance is improved, immunocompetence of the animals can be improved remarkably, death and culling rate is reduced, absorption of the animals to the feed is accelerated, reproductive performance of sows is improved, the number of alive piglets is increased, and economic benefit is also increased.

The present invention discloses fermentation accessory ingredients for preparing pickled vigna unguiculata and a natural fermentation technological method of the pickled vigna unguiculata. The fermentation accessory ingredients consist of the following raw materials in percentages by weight: 1-3% of white rock candy, 4-8% of pickling salt, 2-6% of edible salt, 2-5% of fresh gingers, 2-5% of garlic, 0.3-1.5% of Chinese wolfberry fruits, 0.5-2.0% of dried red chilies, 0.2-1.0% of Chinese prickly ash, 0.5-1.0% of star anise, 0.5-1.0% of cassia bark, 0.1-0.5% of radix notoginseng powder, 0.1-0.3% of bay leaves, 0.2-0.8% of soybean polypeptide powder and the balance water. The method is as follows: the naturally air-dried vigna unguiculata and the fermentation accessory ingredients at a ratio of 30-50% are soaked in a fermentation vessel and the vigna unguiculata is completely immersed in the fermentation accessory ingredients; and the fermentation vessel is sealed, the vigna unguiculata is subjected to sealed natural fermentation for 12-16 days, and the fermented vigna unguiculata is can be eaten. The method is simple and convenient in operations and free of addition of calcium chloride, and can realize industrialized production. The prepared pickled vigna unguiculata by the method is yellow and bright in color, pleasant in flavor, mellow in acid taste, crisp and refreshing in mouthfeel, and low in the content of nitrite.

The invention belongs to the technical field of cultivation of vigna unguiculata, and particularly relates to an open-field cultivation method for a medium-maturing sprawling short vigna unguiculata variety in summer in south China. The open-field cultivation method for the medium-maturing sprawling short vigna unguiculata variety in the summer in the south China comprises the following steps: (1)selecting the short vigna unguiculata variety suitable for planting in the summer: selecting Gui vigna unguiculata No.2 as the short vigna unguiculata variety; (2) performing soil disinfection with solarization for two times; (3) selecting an application method of a base fertilizer and topdressing; (4) performing seed treatment; (5) performing reasonable final singling; (6) performing sowing andperforming field planting; (7) performing pruning; and (8) performing water management. The method provided by the invention adopts a combination manner of two-time deep fertilization and foliage top-dressing, primary fertilization is performed before the vigna unguiculata is sown and a fertilizer is applied as the base fertilizer, secondary fertilization is performed in the early flowering periodof the vigna unguiculata, and compared with a local custom fertilization method of vigna unguiculata, the method provided by the invention saves fertilizers, saves labor, has a high yield, excellentquality and high efficiency; a secondary deep application method is deep application in a soil layer, volatilization and loss of the fertilizers are reduced; and in the middle and late stage of flowering and podding of the vigna unguiculata, topdressing of atonik and an appropriate compound fertilizer is combined, so that premature senescence in the late growth stage is prevented, a harvesting period is prolonged by 20 days, and a yield is improved.

The invention relates to an indoor soilless culture relay intercropping method with the pest killing effect. The indoor soilless culture relay intercropping method includes the following steps: a, relay intercropping is carried out on six kinds of plants including capsicum frutesces, rumex acetosa, solanum melongena, bidens tripartite, vigna radiate and ranunculus sceleratus in a soilless culture mode indoors; b, the six kinds of seedlings are transplanted, and a gauze cage with bemisia tabaci is placed at the time thirty days after transplanting; c, the number of the dead bemisia tabaci is counted at the time 72 hours after placement and recorded, and the number of the dead plant seedlings is counted at the time forty days after planting and recorded. The indoor soilless culture relay intercropping method mainly solves the problems that the soilless culture seedlings are not high in survival rate and prone to suffering pest attacking; the six kinds of plant seedlings are selected for relay intercropping, the survival rate of the seedlings is guaranteed, and the pest killing effect is improved, so that the seedlings can normally grow, and the pest killing characteristic of the seedlings can be further developed. The indoor soilless culture relay intercropping method is simple in action, low in cost, remarkable in pest killing effect and high in seedling survival rate.

The invention relates to vigna radiata oligopeptide powder and a preparation method thereof. The peptide content in the vigna radiata oligopeptide powder is higher than 85 weight percent, wherein themolecular weight of more than 98 percent of vigna radiata peptides is smaller than 1000 Daltons. The preparation method of the vigna radiata oligopeptide powder comprises the steps of protein extraction, biological enzyme enzymolysis, filtration, concentration and drying. Operation work procedures are simple; no pollution is caused to the environment; no harm is caused to human bodies; a production rate is high; and the cost is low. The process is suitable for large-scale production. The oligopeptide powder has the efficacy of high free radical scavenging activity and the like.

The invention discloses a high-throughput-sequencing and host-background-interference-free method for amplifying ITS genes of endophytic fungi of vigna unguiculata. According to the method, a modified oligonucleotide primer combination is adopted, the amplification of host-derived ITS genes of the vigna unguiculata is removed through a multiple PCR amplification method, meanwhile, the amplification of the ITS genes of the endophytic fungi of the vigna unguiculata is not affected, finally, the variety and abundance of the endophytic fungi (including pathogenic microorganisms) of the vigna unguiculata can be surveyed and understood comprehensively through a high-throughput sequencing method, and thus, a reliable new way is provided for identifying the endophytic fungi of the vigna unguiculata. Compared with the traditional methods which comprise the step of carrying out identification after separation and purification, the method has the advantage that the existing technical limitations are overcome. By the method, environmental pollution resulting from complicated operating processes can be reduced, the required sample size is small, the test operation is simple and easy, and the level of research on fungus diseases of the vigna unguiculata can be greatly increased.

The invention discloses an edible fungus noodle, which is composed of the following raw materials in parts by weight: 80 to 90 parts of barley, 45 to 55 parts of vigna eylindrica, 25 to 35 parts of seed of Job's tears, 10 to 15 parts of pumpkin flower, 20 to 25 parts of bolete, 20 to 25 parts of pleurotus nebrodensis, 18 to 24 parts of pholiota nameko, 1 to 2 parts of white glorybower, 1 to 3 parts of hawthorn, 1 to 2 parts of viola inconspicua, 1 to 2 parts of hollyhock root, 1 to 2 parts of noodle additive, and a proper amount of water. The preparation method takes barley, vigna eylindrica, and seed of Job's tears as the main raw materials; wherein the barley has the functions of treating diabetes, removing heat toxin, tonifying qi and the middle, relieving the fatigue, strengthening the blood vessel, moistening the skin, and relieving the hunger; the vigna eylindrica contains following components: protein, fat, sugar, phosphor, calcium, iron, zinc, vitamin B1, vitamin B2, nicotinic acid, pantothenic acid, stigmasterol, phosphatide, cyanophoric glycoside, blood coagulation factor, and the like; the bolete has the functions of fortifying the spleen, removing the dampness, clearing the heat, relieving the temper, nourishing the blood, regulating the middle, expelling the wind and cold, relaxing the muscles, activating the blood, tonifying deficiency, and refreshing; and the pleurotus nebrodensis has the functions of removing food retention, killing parasites, relieving cough, modulating the human physiological balance, and strengthening the human immunity.

The invention discloses a rehydration and brewing method of vigna unguiculata half-dried products. The method comprises the following steps of performing rehydration, preparing vegetable pickling water, loading the prepared vegetable pickling water in a jar, and performing fermentation so as to prepare the vigna unguiculata half-dried products. After being rehydrated, vigna unguiculata low-salt half-dried products which are obtained after infrared radiation drying are favorable in quality and suitable for pickling processing. After the vigna unguiculata low-salt half-dried products are fermented in Laotan vegetable pickling water, the salinity is moderate and the flavor, the texture and the color are good. The rehydration and brewing method disclosed by the invention realizes the rehydration and the fermentation of the vigna unguiculata half-dried products after infrared drying, a production technology of low-salt fermentation vegetables is provided, and the purpose of low-salt production of conventional fermentation vegetables and reduction of the generation of high-salt waste water are realized.

The present invention belongs to the technical field of agriculture and specifically relates to a eucalyptus planting method in a rare earth mining area, and the method has a significant effect for the treatment of sulfate ions and can control water loss and soil erosion. The eucalyptus planting method is characterized by comprising a step of carrying out relay cropping of broadleaf paspalum, vigna sesquipedalis or lespedeza bicolor between the eucalyptus when eucalyptus tissue culture seedlings are planted for two months and grow to a height of about 1m. The method specifically includes the steps of: digging and loosing surface soil, directly sowing certain seeds, then covering the seeds with a small amount of garbage soil, and dibble planting 1 to 15 clumps every 1.2 m. The eucalyptus has an obvious trunk, can grow to a height of about 8m in two years, and can rapidly form an upper canopy. The eucalyptus has sparse branches and leaves, and can form a eucalyptus forest with a large transmittance. Moreover, the broadleaf paspalum, the vigna sesquipedalis, the lespedeza bicolor and the like have certain shade tolerance. Therefore, the broadleaf paspalum, the vigna sesquipedalis, the lespedeza bicolor and the like can form a stable tree, shrub and grass mixed vegetation community with the eucalyptus.

The invention discloses a method for planting vigna unguiculata. The method comprises the following steps: carrying out vigna unguiculata seed selection: selecting high-quality seeds; carrying out seed pretreatment: carrying out sterilization and soaking; carrying out vigna unguiculata seedling raising: subjecting the seeds to disinfecting, soaking and constant-temperature seedling raising; carrying out land preparation, fertilizer application and seedling transplanting: carrying out land parcel partitioning according to specifications, and carrying out seedling transplanting; carrying out field management: carrying out intertillage, topdressing and insect prevention; and carrying out vigna unguiculata picking: carrying out picking generally one month after seedling transplanted growth in accordance with the standards that the vigna unguiculata is plump and seeds just become visible. According to the method, the planting of the vigna unguiculata is started on the basis of seed pretreatment and seedling raising, and intertillage soil loosening, topdressing and insect-prevention pesticide spraying are carried out according to growth conditions of the vigna unguiculata during planting, so that the vigna unguiculata can be not prone to diseases during planting, the resistance is relatively good, finally, high yield is achieved, and then, the income of farmers can be better.

The invention aims to disclose a healthcare fermented broad bean containing Hosta ventricosa, and a making method thereof. The healthcare fermented broad bean is composed of 55-65 parts of broad bean, 8-12 parts of Hosta ventricosa, 6-8 parts of Villous Amomum Fruit, 5-7 parts of stir-baked medicated leaven, 4-7 parts of malt, 1-1.5 parts of leaf of Vigna sinensis, 0.8-1.2 parts of leaf of Prunus Humilis Bunge, 0.6-1 part of Rhodiola rosea, 2-3.2 parts of Chinese prickly ash, 8-10 part of rapeseed oil, 8-10 parts of a yellow rice wine, 10-14 parts of salt, and 6-8 parts of garlic. The fermented broad bean produced in the invention is delicious and tasty and has a unique fragrance, the added Hosta ventricosa can move qi and relieve pains, has a very good effect on abdominal distention and pains caused by qi stagnation in the spleen and the stomach, and can also rectify qi, course the liver, invigorate the spleen, eliminate stagnation, and adjust the qi stagnation in the stomach and the intestine, and Villous Amomum Fruit can move qi, relieve epigastric distention, strengthen the stomach and promote the digestion. The healthcare fermented broad bean containing Hosta ventricosa also contains a plurality of traditional Chinese medicinal components beneficial for human bodies, and is beneficial for the human bodies through long-term eating.

The invention provides a planting technology of vigna unguiculata, and belongs to an agricultural planting technology. The technology comprises preparing land, treating seeds, sowing, performing field management, and harvesting in property time. Using the planting technology to plant vigna unguiculata, yield of the vigna unguiculata is more than 30% higher than yield of a conventional technology.

The invention discloses a nutritional feed for meat rabbits and a preparation method and particularly belongs to the technical field of feed preparation. The nutritional feed for the meat rabbits comprises the following raw materials in parts by weight: 20 parts of maize meal, 20 parts of purple alfalfa, 30 parts of wheat bran, 7 parts of carrot seedlings, 10 parts of sweet potato flour, 0.5 partof magnesium sulfate, 1 part of tricalcium phosphate, 1 part of sodium selenite, 10 parts of vigna unguiculata, 10 parts of chicory, 15 parts of bamboo shoots, 15 parts of arrowheads, 8 parts of Rhodiola rosea, 15 parts of Chinese artichokes, 23 parts of purple rice flour, 8 parts of common salt, 6 parts of pagoda tree flowers, 9 parts of rhizoma dioscoreae, 5 parts of Chinese herbal medicine mixture and 1 part of trace element. According to the feed, the raw materials are purely natural, the formula is reasonable, the nutrition is rich and balanced, thus, the taste of the feed can be improved, the food calling ability is enhanced, the appetite of the meat rabbits is improved, the digestibility is enhanced, the quality of meat and meat yield are improved, meanwhile, the tolerance, digestibility and absorptivity of intestinal tracts of the rabbits to starch and fats can be improved, the incidence of diseases is reduced, the culture cost is reduced, and farmers' income is increased.

The invention relates to a method for testing drought tolerances of vigna unguiculata type I and vigna unguiculata type II by fluorescence quantitative PCR, diagnostic genes and primers. According to the method, sequences of the tested diagnostic genes are shown as in SEQIDNO: 1; the fluorescence quantitative PCR primers have the sequences as follows: a forward primer: 5'-TGGATTGTGGTGGACATAC-3' and a downstream primer: 5'-GGTTCCTTCTGAGCATTGA-3'. The method, the diagnostic genes and the primers have the advantages that significant differentially expressed genes in materials of drought tolerances of the vigna unguiculata type I and the vigna unguiculata type II provide a diagnostic gene tool for distinguishing the drought tolerances of different types of vigna unguiculata specifically. In addition, direct technical support is provided for testing drought tolerance types of different breeding parent materials by real-time fluorescence quantitative PCR rapidly and conveniently based on the primer sequences of the tolerance diagnostic genes of the vigna unguiculata type I and the vigna unguiculata type II.

The invention discloses herb tea capable of lowering hyperlipidemia. The herb tea is prepared from the following raw materials in parts by weight: 10-20 parts of crataegus pinnatifida, 5-10 parts of lycium barbarum, 5-10 parts of lotus leaves, 5-10 parts of coix lacryma-jobi, 3-5 parts of siraitia grosvenorii, 10-20 parts of camellia sinensis, 10-20 parts of lonicera japonica, 5-10 parts of vigna radiata, 3-8 parts of semen nelumbinis, 3-5 parts of gardenia jasminoides and 5-10 parts of mel. A preparation method of the herb tea comprises the procedures of carrying out soak-cleaning on the raw materials, baking, crushing, frying, carrying out cooking disinfection, mixing and boiling. The preparation method is simple and is rich in source of raw materials, low in cost, high in Chinese herbal medicine active ingredient extraction ratio and high in product quality. The herb tea capable of lowering hyperlipidemia, disclosed by the invention, is prepared according to the prescription principle of the traditional Chinese medical science on the basis of the health preserving theory and treatment principle of the traditional Chinese medicine, can generate a synergistic effect, is used for treating both deficiency and excess and giving attention to both manifestation and root cause, can be used for supplementing the cell vitality of a human body, can also be used for promoting the ecological balance of the human body, and has the effects of reducing blood fat, reducing pathogenic fire, removing dryness, simultaneously treating a variety of diseases and caring health.

The invention discloses a molecular marker SNP#2 for identifying powdery mildew resisting phenotype of vigna radiata, and a primer and application of the molecular marker SNP#2. A primer pair of the molecular marker SNP#2, namely SEQID NO.1 and SEQID NO.3, can amplify a characteristic strip of 418bp in vigna radiata varieties susceptible to powdery mildew, and cannot amplify the characteristic strip in the vigna radiata varieties resisting powdery mildew; and a primer pair of the molecular marker SNP#2, namely SEQID NO.2 and SEQID NO.3, can amplify a characteristic strip of 418bp in vigna radiata varieties resisting powdery mildew, and cannot amplify the characteristic strip in the vigna radiata varieties susceptible to powdery mildew. The powdery mildew resisting site of the vigna radiatais positioned, so that the inventor finds the molecular marker which is jointly separated from a vigna radiata gene for resisting powdery mildew, and the marker selecting efficiency achieves 100%. The molecular marker primer is used for identifying or screening powder mildew resistance of the vigna radiata. The method is quick and simple, high in selecting efficiency, and low in cost.