16 results about "Vigna" patented technology

The invention discloses a cowpea 5K SNP (Single Nucleotide Polymorphism) liquid chip and application thereof in variety identification, and belongs to the technical field of biology. The liquid chip provided by the invention comprises a set of nucleotide probes for detecting 5847 SNP sites of cowpea, and can be widely applied to different scenes of germplasm resource identification, genetic diversity evaluation, variety identification, genetic relationship identification, purification and rejuvenation of local varieties and the like of different cowpea varieties; an effective scientific basis is provided for cowpea variety identification, the variety identification and evaluation efficiency is improved, and the method has a remarkable application value.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300126, a KASP type molecular marker 300126 closely linked with the cowpea salt tolerance major QTL is located at the position of 43816166 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.

The present application provides isolated regulatory genome sequences of legume plants, preferably Glycine, Pisum, Cicer plants, such as Glycine max, Pisum sativum, Vigna radiata, Vicia faba, Phaseolus vulgaris, Cicer arietinum, and uses thereof, as shown in SEQ ID NO: 3. The present application has important application value for developing close-planting soybeans, intercropped soybeans, etc., and has great value for optimizing the architecture of soybean plant type and improving the yield of soybeans per unit area related to theoretical and practical research.

The application provides a cultivation management method for promoting growth of amomum villosum, relates to the technical field of amomum villosum planting, and adopts composted manure, fermented material of vigna unguiculata, biochar, completely gelatinized starch, conidia of lecanimigma verrucosum, superphosphate, urea and the like to prepare a solid fertilizer for fertilization management in spring and autumn. The application overcomes the shortcomings of the prior art, effectively guarantees the growth of amomum villosum stems and further promotes early flowering and fruiting of amomum villosum, and comprehensively improves the economic value of artificial planting of amomum villosum.

The invention discloses a cowpea heat resistance major QTL (quantitative trait loci) closely linked molecular marker as well as a primer and application of the cowpea heat resistance major QTL closely linked molecular marker. The cowpea heat resistance major QTL is named as HS-Pn-1. 1, and a KASP molecular marker 300175 closely linked with the cowpea heat resistance major QTL is located at the 1157933 bp position of a chromosome 1. The marker is used for performing PCR amplification on a to-be-detected material, and whether a sample carries heat resistance key allelic variation or not can be accurately judged according to genotypes. By means of the molecular marker and the primer, efficient and accurate screening of the cowpea heat resistance main effect QTL can be achieved, compared with a traditional phenotype identification method, the method has the advantages of being high in detection flux, stable and reliable in result and the like, the typing accuracy can reach 99% or above, the breeding efficiency of cowpea heat resistance varieties can be remarkably improved, and the breeding period can be effectively shortened.

The invention discloses a lactobacillus rhamnosus C and application thereof in fermentation of red vinasse acid vigna unguiculata, and belongs to the technical field of microorganisms. The preservation number of the lactobacillus rhamnosus C disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) NO.32918. The lactobacillus rhamnosus C disclosed by the invention has the advantages that the preservation number is CGMCC NO.32918; the strain has excellent acid production capacity and nitrite degradation capacity, when the strain is used for artificial inoculation and fermentation of red vinasse pickled vigna unguiculata, a red vinasse pickled vigna unguiculata product which is excellent in quality, unique in flavor and high in food safety can be produced, and the fermentation period is remarkably shortened. The strain also has salt resistance, acid resistance and ethanol resistance, the high resistance enables the strain to be widely used, and a new microbial resource is provided for lactic acid bacteria and subsp. Rhamnosus.

This invention relates to the field of agricultural planting technology, specifically a method for improving perennial cool-season grasslands through differentiated mixed sowing and fertilization of annual forage grasses in multiple seasons, comprising the following steps: S1, grassland selection; S2, sowing and mowing times: three sowing times are set, namely May, August, and November of each year; S3, sowing ratio and sowing amount: sowing amount: the differentiated combination sowing amount is determined according to the degree of degradation of perennial grass grasslands and the season, and the sowing amount in May and August is: rye / oats 60-65 kg / hm. ‑2 Variegated purple vetch / arrow-leaved pea 45-50 kg / hm ‑2 The sowing rate in November is 45-50 kg / hm² for rye / oats. ‑2 40-45 kg / hm of *Vigna angularis* var. *gloriosa*. ‑2 Sowing time: In May and August, sowing is generally done after an effective rainfall when the soil moisture is suitable. Break the sod and sow shallowly for 1-2 cm. In November, sowing is done before rainfall, with a sowing depth of 2-3 cm. S4. Fertilization.

The application discloses a molecular marker closely linked with a main-effect QTL of salt tolerance of cowpea and primer sequences of the molecular marker. The main-effect QTL of salt tolerance of cowpea disclosed by the application is named SC3_00126, and a KASP type molecular marker 3_00126 closely linked with the main-effect QTL is located at a position of 43816166 bp on chromosome 11. The marker can be used for amplifying to-be-tested materials, and whether the sample carries a key allelic variation for controlling salt tolerance can be judged according to the genotype. The molecular marker and the primer provided by the application can be used for rapidly screening the main-effect QTL of salt tolerance of cowpea, and compared with a traditional phenotype identification method, the application has the advantages of high detection flux, accurate and reliable results and the like, and the genotyping accuracy can reach more than 99%. The technology can significantly improve the breeding efficiency of salt-tolerant varieties of cowpea and shorten the breeding period.

The invention belongs to the technical field of plant cultivation, and particularly relates to a method for germinating aeschynomene indica seeds of leguminous plants. The method comprises the following steps: selecting field mature aeschynomene indica seeds, drying, threshing, grinding with fine sand, slightly scratching seed coats to ensure that embryo parts are not damaged, carrying out heat treatment on the scratched seeds, putting into deionized water, and germinating under the conditions of illumination for 12 hours and darkness for 12 hours. The method can effectively improve the germination efficiency of the aeschynomene indica seeds.

The invention relates to a method for producing nesidiocoris tenuis, which is characterized in that under the conditions that the temperature is 18-35 DEG C and the humidity is 60-90% RH, vigna unguiculata is independently utilized to produce the nesidiocoris tenuis, or the vigna unguiculata is combined with carpoglyphus lactis and fruit fly larvae to produce the nesidiocoris tenuis. The invention proposes for the first time, and unexpectedly finds that the expanding propagation production of the nesidiocoris tenuis can be realized by independently utilizing the vigna unguiculata, and the population of the nesidiocoris tenuis can be continuously increased; if carpoglyphus lactis is added in the feeding process, the feeding effect of the drosophila larvae is better. According to the method, a brand new scheme can be provided for the production of the nesidiocoris tenuis, lepidoptera eggs are used for producing the nesidiocoris tenuis to lay eggs in the early stage, no other food is added in the later stage, the vigna unguiculata with the eggs are used for producing the nesidiocoris tenuis, and the production cost can be remarkably reduced.

The invention discloses a preparation method of a broad bean leaf extract and a product and application thereof, and the preparation method comprises the following steps: S1, freeze-drying fresh broad bean leaves, crushing and grinding to obtain broad bean leaf powder; s2, adding the broad bean leaf powder into a hydrochloric acid aqueous solution for ultrasonic extraction to obtain a mixture; s3, centrifuging the mixture, and collecting supernate, namely the broad bean leaf extract. The content of levodopa in the broad bean leaf extract is detected through high performance liquid chromatography, it is found that the content of levodopa in the broad bean leaf extract is higher than that of other broad bean part extracts, and the broad bean leaf extract can be used for preparing the medicine for treating and improving the Alzheimer's disease. The technical support is provided for extending the broad bean industry chain and promoting the rapid development of the broad bean deep processing industry.

Provided is a method of manufacturing a health functional food, which includes step (S1) of preparing a red ginseng fermentation powder mixed with red ginseng, black ginseng, ginger, bellflower, and jujube, step (S2) of preparing a medicinal powder mixed with Bikal skullcap (Scutellaria baicalensis), Amur Corktree (Phellodendron amurense), gardenia (Gardenia jasminoides), Purslane (Portulaca oleracea), Chameleon plant (Houttuynia cordata), tea plant (Camellia sinensis), Beefsteak plant (Perilla frutescens var. crispa), Cuscuta chinensis Lam, Cistanche Deserticola, Asparagus cochinchinensis, rehmania root (Rehmannia glutinosa), steamed rehmania root, Thuja Orientalis Seed (Seed of Platycladus orientalis fruit), Rubus coreanus, Reishi mushroom (Ganoderma lingzhi), and Acorus gramineu, step (S3) of preparing a grain mixture mixed with rice, white rice barley (Hordeum distichon), glutinous rice, corn, Foxtail millet (Setaria italica), black sesame, perilla, Coix lacryma-jobi, brown rice, sorghum, fermented soybean, Canavalia ensiformis, chickpea (Cicer arietinum), buckwheat, green gram (Vigna radiata), and oat, step (S4) of preparing a vegetable mixture mixed with sea bamboo shoot, carrot, burdock root, lotus root, cinnamon, water parsley (Oenanthe), broccoli, kelp, and cabbage.

The invention belongs to the technical field of pathogenic bacterium detection, discloses a kit for detecting cowpea fusarium wilt based on dual RPA-LFD and application, and particularly discloses a reagent which comprises a primer pair and a probe. The invention designs and screens the optimal primer pair and probe for fusarium oxysporum and phytophthora vigna of the cowpea fusarium wilt, and the reagent containing the primer pair and probe can realize better detection results in single and multiple detections of fusarium oxysporum and phytophthora vigna, and also has higher accuracy in the detection of actual samples. Compared with the current common technology for detecting the cowpea fusarium wilt, the reagent provided by the invention can be used for rapidly and accurately detecting fusarium oxysporum and cowpea phytophthora of the cowpea fusarium wilt, does not need to depend on professionals, can be used for detecting the cowpea fusarium wilt under a constant-temperature condition, and is very suitable for field detection of the cowpea fusarium wilt.

The application discloses a heat-resistant major QTL closely linked molecular marker of cowpea, primers thereof and application. The heat-resistant major QTL of the cowpea is named HS-Pn-1.1, and the KASP molecular marker 3_00175 closely linked therewith is located at the position of 1157933 bp of the No.1 chromosome. The sample whether carrying a key allelic variation of heat resistance can be accurately determined according to the genotype by carrying out PCR amplification on the to-be-tested material through the marker. The molecular marker and the primers provided by the application can realize efficient and accurate screening of the heat-resistant major QTL of the cowpea. Compared with a traditional phenotype identification method, the method has the advantages of high detection flux, stable and reliable results and the like, the typing accuracy can reach more than 99%, and the technology can significantly improve the cowpea heat-resistant variety breeding efficiency and effectively shorten the breeding period.

This invention provides an anti-wrinkle composition containing extracts of *Velvetia scabra* flowers and its preparation method. The anti-wrinkle composition includes extracts of black willow bark, *Velvetia scabra* flowers, *Inula japonica* flowers, *Edelweiss* extract, and adenosine. When the above raw materials are combined in the composition, they can synergistically promote the secretion of type I collagen, and have significant effects in improving skin elasticity and reducing the formation of wrinkles.

The invention discloses a molecular marker closely linked with a cowpea salt tolerance major QTL (quantitative trait loci) and a primer sequence of the molecular marker. The name of the cowpea salt tolerance major QTL is SC300129, the KASP type molecular marker 300129 closely linked with the cowpea salt tolerance major QTL is located at the position of 43818260 bp on a chromosome 11, the marker can be used for amplifying a material to be detected, and whether a sample carries key allelic variation for controlling salt tolerance or not is judged according to the genotype of the marker. The molecular marker and the primer provided by the invention can be used for quickly screening the cowpea salt tolerance major QTL, and compared with a traditional phenotype identification method, the molecular marker and the primer have the advantages of high detection flux, accurate and reliable result and the like, and the typing accuracy can reach 99% or above. The technology can significantly improve the breeding efficiency of the salt-tolerant variety of cowpea and shorten the breeding period.