26 results about "Vicia faba" patented technology

Provided are a quality-improving and nitrogen-fixing coupled microbial agent and the use thereof. The quality-improving and nitrogen-fixing coupled microbial agent is a microbial composition, has coupled functions of improving quality and fixing nitrogen so as to improve the quality of products of leguminous crops, and has the functions of regulating and increasing the rhizobial abundance for the rhizosphere of leguminous crops and increasing the amount of nodules of leguminous crops. The microbial agent contains all gene sequences of nucleotide sequences shown as SEQ ID NO.1-12 or four or more gene sequences among same. When being applied to production of leguminous crops, such as soybeans, peanuts, peas, broad beans and cowpeas, the quality-improving and nitrogen-fixing coupled microbial agent has coupled functions of improving quality and fixing nitrogen and achieves quality improvement and nitrogen fixation and green yield increase. The microbial agent is easy to use, achieves remarkable social and ecological benefits, and facilitates popularization and use.

The present application provides isolated regulatory genome sequences of legume plants, preferably Glycine, Pisum, Cicer plants, such as Glycine max, Pisum sativum, Vigna radiata, Vicia faba, Phaseolus vulgaris, Cicer arietinum, and uses thereof, as shown in SEQ ID NO: 3. The present application has important application value for developing close-planting soybeans, intercropped soybeans, etc., and has great value for optimizing the architecture of soybean plant type and improving the yield of soybeans per unit area related to theoretical and practical research.

The invention discloses a weed pathogenic bacterium spiropolyspora roseum JZ-28 and application thereof in weed prevention and removal, and belongs to the technical field of microorganisms. The weed pathogenic bacterium, namely the clonostachys rosea JZ-28, is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.41916, and the preservation date is April 15, 2025. The invention further discloses a preparation method of the clonostachys rosea. According to the invention, a weed pathogenic bacterium, namely the spiropolyspora pink JZ-28, is obtained through separation. Through identification, the strain has high pathogenicity to four weeds including chenopodium album, elsholtzia densa, malva verticillata and senecio scandens, the incidence rate of living weed plants reaches 70% or above, and the strain is safe to corn, oilseed rape, cucumber, tomato, eggplant, broad bean and pea, has the potential of being developed into a microbial herbicide for broad-leaf weeds, and is wide in application prospect.

The artificial breeding and island plant pollination method comprises the following steps: artificial breeding of aphids: broad bean seedlings are planted in an insect breeding cage to propagate black bean aphids, so that adults of the hoverflies can lay fly eggs, and larvae of the hoverflies can eat the fly eggs at the same time; feeding of hoverfly larvae: feeding the hoverfly larvae with black bean aphids in an insect breeding cage, and enabling the hoverfly larvae to develop into pupae after 6 days; collecting pupae of hoverflies with short thorn legs: harvesting broad bean seedlings with mature larvae of hoverflies, putting the broad bean seedlings into a plastic box with a gauze opening for continuous feeding, and putting the collected pupae of hoverflies into a culture dish until all the larvae in the plastic box pupate; and transporting and releasing the short-spur hoverflies, namely storing pupae of the hoverflies in a pupa card device at the temperature of 4 DEG C, transporting the pupae to an open sea island, and releasing the pupae. By the adoption of the method, scale breeding can be conducted on the species of the hoverfly brachypoda, and the hoverfly brachypoda can be transported to the island area and used for pollination of island plants.

The present invention belongs to the field of microorganisms. Specifically, disclosed are an ARC microbial agent for quality improvement, nitrogen fixation and green yield increase, and the use thereof. The ARC microbial agent for quality improvement, nitrogen fixation and green yield increase has the effects of improving quality, fixing nitrogen and increasing yield, thereby improving the quality of a leguminous crop product. The ARC microbial agent has the effects of regulating and improving the abundance of rhizobia in the rhizosphere of the leguminous crop and increasing the number of nodules in the leguminous crop; and contains gene sequences as shown in SEQ ID NOs: 1-4. The ARC microbial agent can be used in the production of leguminous crops such as soybeans, peanuts, peas, broad beans and cowpeas, thereby realizing quality improvement, nitrogen fixation and green yield increase. In addition, the ARC microbial agent is simple to use, has significant social and ecological benefits, and is easy to popularize and apply.

The plant source insecticide contains effective components of parathion and avermectin, the weight ratio of parathion and avermectin is 10-50:1, and is supplemented with an acceptable agricultural adjuvant to form one of emulsifiable concentrate, wettable powder, water dispersible granule, water emulsion or suspension agent suitable for agricultural use, and belongs to the field of pesticides. The plant source insecticide shows activity on peridroma saucia, aphid and cotton red spider, especially high acaricidal activity on red spider. The prevention and treatment effect on citrus red spider is obviously improved after parathion and avermectin are compounded, which shows that the combination of parathion and avermectin has significant synergistic effect on citrus red spider. Parathion is derived from Chinese herbal medicine plant, and there is no problem of pesticide residue, and the risk of drug damage is greatly reduced. At the same time, the two single agents of the composition have great difference in chemical structure and completely different action mechanism, and there is no cross resistance, which can delay the resistance problem caused by the use of the two single agents alone.

The application provides a cultivation management method for promoting growth of amomum villosum, relates to the technical field of amomum villosum planting, and adopts composted manure, fermented material of vigna unguiculata, biochar, completely gelatinized starch, conidia of lecanimigma verrucosum, superphosphate, urea and the like to prepare a solid fertilizer for fertilization management in spring and autumn. The application overcomes the shortcomings of the prior art, effectively guarantees the growth of amomum villosum stems and further promotes early flowering and fruiting of amomum villosum, and comprehensively improves the economic value of artificial planting of amomum villosum.

The invention relates to an efficient plant genetic transformation vector based on rhizobium rhizogenes, which is modified on the basis of a pTi vector, and a left boundary pTi-LB and a right boundary pTi-RB of T-DNA of the vector are respectively replaced by a left boundary pRi-LB and a right boundary pRi-RB; deleting a Hyg expression cassette in the T-DNA region, and connecting a target gene expression cassette to the front end of the original RUBY expression cassette; a VirGN54D insert element is inserted between an Ori element and an SmR element in a non-T-DNA (Transfer-Deoxyribose Nucleic Acid) region; the 106th site of a RepA protein coding gene sequence in pVS1 of a non-T-DNA region is mutated into a histidine coding sequence from an arginine coding sequence. The invention aims to enhance efficient genetic transformation of plant exogenous genes in multiple species, has broad-spectrum adaptability to plant species, and particularly has obvious effects in multiple plant species which depend on genotypes and are difficult to transform, such as cucumbers, white radishes, broad beans, Chinese little greens, black nightshade, pokeberry roots, gingko, phalaenopsis, Chinese roses, Chinese pines and the like.

The application discloses a chickpea planting device and a planting method thereof. The chickpea planting device comprises a base, a ditching mechanism, a seeding mechanism, a water and fertilizer stirring mechanism, and a soil covering and film covering mechanism. The ditching chain wheel assembly performs a ditching action in the soil under the driving action of a ditching driving motor. The seeding mechanism comprises a seed bin, a seed disc, and a first driving assembly. The seed bin is provided with a seeding gap in communication with a containing cavity. The seed disc is pivotally connected in the containing cavity and is provided with a seeding through hole for containing chickpea seeds. The first driving assembly is connected with the seed disc and is configured to drive the seed disc to rotate in the containing cavity. The water and fertilizer stirring mechanism comprises a water bucket, a stirring assembly, and a spray head assembly. The soil covering and film covering mechanism comprises a bottom plate, a soil covering assembly, and a film covering assembly. The soil covering assembly is configured to fold and cover the soil on both sides of the ditch towards the ditch. The film covering assembly comprises a clamping unit and a film reel connected therewith. The film reel is rotatably released under the clamping of the clamping unit and covers the ditch with the soil.

The present invention relates generally to removing the plant tone of starchy Fabaceae pulses consisting of chickpea (Cicer arietinum), yellow pea (Pisum sativum), common bean (Phaseolus vulgaris) and fava bean (Vicia faba) as hulled or de-hulled pulses, whole pulses, as split pulses or as chopped solids thereof with a Feret diameter (Dmax) of 1 to 4 mm while maintaining the semi-crystalline structure of starch, followed by separating a starch-enriched fraction and a protein and fiber-enriched fraction or a starch-enriched fraction, a protein-enriched fraction and a fiber-enriched fraction.

The invention discloses a seedling and bean collaborative cultivation method for preventing and controlling salt crust in severe soda saline-alkali soil, and relates to the technical field of agricultural planting. The seedling and bean collaborative cultivation method specifically comprises the following steps: S1, land improvement, S2, improvement agent application, S3, ridging and alkali inhibition layer construction, S4, sowing and cultivation, S5, drip irrigation under a film and salt pressing and salt leaching, and S6, straw returning to the field and field management. According to the straw and bean collaborative cultivation method for preventing and controlling salt crust in the severe soda saline-alkali soil, by introducing composite conditioners such as straw charcoal, organic fertilizer, an acid conditioner and a water-retaining agent, the structure, fertility and water-retaining capacity of the saline-alkali soil are remarkably improved; the soil jacking capacity of gramineous crops is utilized, salt crust is broken through firstly, crack channels are formed, leguminous crops such as soybeans are intercropped between gramineous strip rows, the leguminous crops smoothly emerge through cracks, and the emergence rate is increased by 70% or above; and through the alkali inhibition layer at the bottom of the ridge body and drip irrigation under the film, the salt rising is reduced, and the synchronization of salt pressing and salt discharging is realized.

The present invention relates to the field of legume protein isolates, specifically to faba bean protein isolates, and the solubility of such isolates is less than 25% at a pH value higher than or equal to 7. The present invention also relates to its production method and its industrial applications.

The invention discloses a visual detection method for a grape broad bean wilt virus based on RT-LAMP-CRISPR / Cas12a (Reverse Transcription-Amplified Amplification Protein Repeats / Cas12a). The invention develops an RT-LAMP (reverse transcription loop-mediated isothermal amplification)-CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a visual detection method for efficiently detecting the vicia faba wilt virus on the basis of an RT-LAMP (reverse transcription loop-mediated isothermal amplification) technology and a CRISPR / Cas12a (clustered regularly interspaced short palindromic repeats / Cas12a) system. Meanwhile, alkaline resin is creatively adopted for preparing the grape leaf crude extract, and a novel solution is provided for eliminating interference of excessive chlorophyll and other impurities. Experiments prove that the GabV visual detection method established on the basis of RT-LAMP-CRISPR / Cas12a has the characteristics of simplicity, convenience, rapidness, accuracy, high sensitivity, strong specificity and visualization, and a more convenient and efficient technical means is provided for field diagnosis and forecasting work of GabV in China.

The invention discloses a rice planthopper ecological engineering prevention and control method based on nectariferous plant-natural enemy host synergistic configuration. A time sequence continuous nectariferous chain composed of zinnia elegans, coreopsis, sesame and broad beans is constructed on a rice ridge, and a double-belt type spatial layout of a clubmoss host belt on the outer side is combined, so that the number of natural enemy insect populations is increased by 100% or above, the parasitic rate of anagrus nilaparvatae is larger than or equal to 65%, the number of rice planthopper hundreds of insects is controlled to be 400 or below, and the relative control effect in the whole growth period is 70-90%. According to the method, by constructing a farmland ecological project, nectariferous plants and shelters are provided for natural enemy insects, and the number of natural enemy populations is remarkably increased, so that breeding and harm of rice planthoppers are effectively inhibited, dependence of chemical pesticides is reduced, and ecological safety of rice production is achieved.

This utility model discloses a ladybug hatching device, belonging to the field of hatching devices. It includes a hatching base and a hatching cover inserted at the top of the hatching base. The hatching cover contains hatching components for hatching ladybug eggs. Each hatching component includes multiple egg-hatching trays (I), each with an egg-hatching tray (II) above it. Each egg-hatching tray (I) has a sliding plate at its bottom, and broad bean seedlings are cultivated inside. Each egg-hatching tray (II) contains a flat substrate bag. The front surface of the hatching cover has multiple mounting openings adapted to the sliding plate and egg-hatching trays (II). This utility model, by using multiple egg-hatching trays (I and II) to hatch ladybug eggs, can increase the hatching volume. Furthermore, both egg-hatching trays (I and II) can be pulled out, facilitating the quick removal of a certain number of eggs and newly hatched larvae by workers, saving time in picking out newly hatched larvae, and thus improving the hatching efficiency of ladybug eggs.

The invention relates to a pyrido [1, 2-a] pyrimidine mesoion derivative containing an alkynyl ferrocene structure as well as a preparation method and application of the pyrido [1, 2-a] pyrimidine mesoion derivative. The compound disclosed by the invention has a structure shown in a formula [I], has excellent insecticidal activity on spodoptera frugiperda, ostrinia nubilalis, cutworms, plant hoppers, aphis avenae and the like, and can be used for preparing drugs or medicaments for preventing and treating pests such as spodoptera frugiperda, ostrinia nubilalis, cutworms, sogatella furcifera, brown planthoppers, aphis avenae and the like. The structure and the preparation process are simple, and the production cost is low.

The invention discloses a breeding method for local generation-adding breeding of vernalization-free broad bean breeding filial generations, and relates to the technical field of crop breeding, and the breeding method comprises the following steps: planting and preliminary screening in autumn, maturity screening in winter, and generation-adding and screening in spring; according to the method, vernalization-free broad bean characteristics and a facility cultivation technology are integrated, dependence of traditional broad bean breeding on low-temperature vernalization is successfully overcome, generation adding is achieved in a local greenhouse through vernalization-free parent filial generations, conventional one-year single-season breeding is compressed into one-year double-season circulation, a three-stage time sequence screening mechanism is accurately set, and the breeding efficiency is improved. According to the method, vernalization-free premature genes are directionally enriched, the breeding accuracy is remarkably improved, meanwhile, the geographical limitation of generation adding in different places is avoided, a novel efficient, low-cost and localized broad bean breeding normal form is finally formed, and a core support is provided for breeding of fresh-eating broad bean premature varieties.

The invention provides a cultivation management method for promoting growth of fructus amomi, and relates to the technical field of fructus amomi planting.The cultivation management method includes the steps that decomposed manure, broad bean vine fermentation materials, biochar, completely gelatinized starch, verticillium lecanii leavening agents, calcium superphosphate, urea and the like are adopted for preparing solid fertilizer, and fertilization management is conducted in spring and autumn. According to the method, the defects in the prior art are overcome, the growth of the stems of the fructus amomi is effectively guaranteed, early flowering and fruiting of the fructus amomi are further promoted, and the economic value of artificial planting of the fructus amomi is comprehensively improved.

The invention discloses a two-stage artificial breeding method for Harpactor fuscipes, and belongs to the technical field of biological control of agricultural pests, and the method comprises the following steps: breeding bean aphids by planting broad beans, and breeding newly hatched Harpactor fuscipes by using the bean aphids; then mulberry leaves are used for breeding silkworms, 1-3-year-old silkworm larvae are used for breeding 2-5-year-old sacharius fuscipes nymphs, the sacharius fuscipes nymphs and adult sacharius fuscipes are used for hunting lepidoptera pests in an orchard, and harm of the lepidoptera pests to fruit trees is reduced. The method utilizes low-value materials, is low in cost and simple to operate, can effectively reduce the population density of lepidoptera pests in the orchard and reduce fruit loss of the orchard, and has a wide application prospect in the field of orchard pest control.

The invention discloses a preparation method of a broad bean leaf extract and a product and application thereof, and the preparation method comprises the following steps: S1, freeze-drying fresh broad bean leaves, crushing and grinding to obtain broad bean leaf powder; s2, adding the broad bean leaf powder into a hydrochloric acid aqueous solution for ultrasonic extraction to obtain a mixture; s3, centrifuging the mixture, and collecting supernate, namely the broad bean leaf extract. The content of levodopa in the broad bean leaf extract is detected through high performance liquid chromatography, it is found that the content of levodopa in the broad bean leaf extract is higher than that of other broad bean part extracts, and the broad bean leaf extract can be used for preparing the medicine for treating and improving the Alzheimer's disease. The technical support is provided for extending the broad bean industry chain and promoting the rapid development of the broad bean deep processing industry.

The present invention relates to a plant efficient genetic transformation vector based on rhizobium rhizobium, which is modified based on the pTi vector, and its T-DNA left border pTi-LB and right border pTi-RB are replaced with the left border pRi-LB and the right border pRi-RB respectively; its T-DNA region is deleted. Hyg expression cassette, while in its original RUBY The front end of the expression cassette is connected to the target gene expression cassette; in its non-T-DNA region Ori Components and SmR Insert between components VirGN54D insert The present invention aims to enhance the efficient genetic transformation of exogenous plant genes in multiple species, with broad adaptability across a wide range of plant species, particularly in genotype-dependent and difficult-to-transform plant species such as cucumber, white radish, broad bean, cauliflower, nightshade, pokeweed, ginkgo, phalaenopsis, rose, and Chinese pine.

The utility model relates to the technical field of seedling raising device, concretely speaking is a kind of vicia faba seedling raising device with photosynthesis. Including illumination part and seedling raising part, the lower side of illumination part is provided with support frame, and seedling raising part is set to the inside of support frame;Seedling raising part includes outer box body and inner box body, and the inside of outer box body is provided with support layer and partition layer, and inner box body is placed on the upside of support layer, one end of partition layer is connected with through cavity, and the upper portion of through cavity is through with the upside of support layer, and the bottom of through layer is paved with circulating water pipe, and circulating water pipe is paved along the downside of support layer, and the outer end of circulating water pipe is bent and paved to the inside upper portion of outer box body upwards, and the outer end opening of circulating water pipe faces the upside of partition layer, and nutrient agent on the upside of circulating layer flows through through cavity and circulating water pipe, realizes the transverse circulation flow of nutrient agent, and not static soak, this ensures that nutrient agent distribution is more uniform, and nutrient concentration is stable.

1. The name of the design product: pet water dispenser (Tianchi caterpillar bean). 2. The use of the design product: for pets to drink water. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view 1.

The present application relates to a method for large-scale breeding of the cotton aphid braconid. The method comprises the following steps: 1) planting Vicia faba until emergence to obtain Vicia faba seedlings; 2) inoculating Vicia faba aphids onto the Vicia faba seedlings until the number of Vicia faba aphids increases to 4-7 times the inoculation number; 3) inoculating mated cotton aphid braconid females into the device to allow the females to lay eggs in the Vicia faba aphids, thereby obtaining Vicia faba aphids parasitized by cotton aphid braconid eggs; 4) continuing to feed the Vicia faba aphids parasitized by cotton aphid braconid eggs with Vicia faba seedlings until the Vicia faba aphids are immobilized to form aphid mummies after being parasitized; 5) cutting the Vicia faba leaves with the aphid mummies, drying the Vicia faba leaves, and then collecting only the dried leaves with aphid mummies after the live aphids on the Vicia faba leaves that do not form aphid mummies die or escape, and incubating to allow the cotton aphid braconid to emerge as adults.

The invention relates to a pyridino [1, 2-a] pyrimidine mesoion derivative containing an alkynyl cyano structure as well as a preparation method and application of the pyridino [1, 2-a] pyrimidine mesoion derivative. The compound disclosed by the invention has a structure shown in a formula [I], has excellent insecticidal activity on spodoptera frugiperda, prodenia litura, beet armyworm, ostrinia nubilalis, cutworm, plant hopper, green peach aphid, aphis avenae and the like, and can be used for preparing drugs or medicaments for preventing and treating pests such as spodoptera frugiperda, prodenia litura, beet armyworm, ostrinia nubilalis, cutworm, plant hopper, green peach aphid and the like. The structure and the preparation process are simple, and the production cost is low.