56 results about "Verticillium lecanii" patented technology

The invention relates to a Paenibacillus polymyxa Jaas ed2 with the functions of disease prevention and growth promotion to crops and application thereof, and belongs to the technical field of intensive agriculture production. The bacterial strain Jaas ed2 adopted by the invention has been preserved at the General Microbiological Culture Collection Center of China Microbiological Culture Collection Administration Committee on Sep. 18th, 2012 with the preservation number of CGMCC No.6580. The bacterial strain has the antagonistic activity to verticillium lecanii of eggplant, oxysporum of watermelon and other various pathogenic fungi, can enter plants of eggplants and watermelons in case of lower concentration of bacterium for colonization and conduction in the crops, can be used for promoting growth and preventing diseases of crops, and particularly has excellent control efficiency to soil-borne vascular bundle diseases, is low in production cost, simple to operate, facilitates ecological environment protection for farmlands, and reduces pesticide residue when used as a preparation, thereby meeting requirements for production of nuisance free agricultural products in China.

The invention discloses a compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and a preparation method thereof. The compound biotic insecticide is mainly mixed through the fermentation liquor of bacillus thuringiensis and the meristem of the verticillium lecanii or blastospore and the aconite alkaloid with proper auxiliary agent according to certain proportion, wherein the mass percentage of the main medicine and the auxiliary agent respectively accounts for 50 percent to 90 percent and 10 percent to 50 percent. The compound insecticide has the advantagesthat the compound insecticide is broad-spectrum, has control effect to various crop pests, and particularly has obvious effect to homoptera pets, lepidoptera pets and coleopteran pets; the desinsection speed is high; the lasting period is long; pets are difficult to develop drug resistance; the compound insecticide is safe to people and animals, environment and natural enemies; the manufacturing process is simple, the material sources are wide, and the cost is low; and the preparations are multiple, and the application field is wide.

The invention relates to a verticillium lecanii pesticide as well as the application thereof, and belongs to the plant protection and pesticide technology field. The bacterial strain provided by the invention is verticillium lecanii K1207 with CGMCC No.2326. The invention produces the bacterial strain via liquid and solid two-phase fermentation, and is characterized in that a wheat bran and bran coat culture medium is adopted as the solid culture medium. The cultured product can be added with and mixed with a wetting agent, a bulking agent and a stabilizing agent to obtain wettable powder after being parched and being crumbled. The inventive verticillium lecanii bacterial strain has high virulence, thereby having good control effects on pests such as green peach aphids, urolcueon formosanums, acyrthosiphon dirhodums, cabbage aphids, cabbage worms, diamondback moths, and greenhouse whiteflies. The materials in fermentation process are cheap and have wide sources, which is suitable for industrialized production without environmental pollution.

The invention discloses a verticillium lecanii quick-release dispersible tablet and a preparation method thereof. Verticillium lecanii concentrated fermentation liquor or dry spore powder thereof is used as a main effective component. Synergist, surface active agent, protective agent, disintegrating agent, wetting agent, filler and lubricant are added as well. The preparation method is implementedby the following steps: 2/3 disintegrating agent and other auxiliary agents, together with verticillium lecanii, are added into a mixer for uniform mixing according to a certain proportion; then thewetting agent with an appropriate amount is added for mixing so that the mixture obtained from the previous step is changed into a wet material; the wet material then undergoes 20-mesh screening so asto be prepared into wet grains; after 24-48-hour drying at the normal temperature in a relatively germfree condition, the rest 1/3 disintegrating agent is added into the dry grains, and then the grains are weighed; the lubricant with a weight accounting for 0.5-0.6% of the grain weight is added for full and uniform mixing, and then the grains are sent to a tablet machine to be pressed into largetablets so that the finished product can be obtained. The verticillium lecanii quick-release dispersible tablet has simple production process and wide material source, is easy for industrial production, can be quickly disintegrated in water to release effective components, takes effect quickly, does not cause dust pollution, has a wide range of prevention and cure and can kill insect and bacteriostasis simultaneously.

The invention discloses a compound desinsection primary agent and a pesticide consisting of verticillium lecanii and abamectin. The primary agent is a mixture of the verticillium lecanii and the abamectin, wherein, the mass ratio between the verticillium lecanii and the abamectin is 100:0.1-0.5; through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pest in particular. The compound pesticide consisting of verticillium lecanii and abamectin of the invention has good effect in preventing and controlling pests such as trialeurodes vaporariorum westwood, bemisa babaci, plant louse, diamond back moth, thrips palmi karny and the like, and has certain effect in controlling mite pests.

The invention discloses a VOCs (volatile organic compounds) purification system for waste gas of petrochemical industry. The system comprises a waste heat exchanger, a gas overheat protection device, a hot gas mass flow meter, a hybrid gas flow regulation valve, a cloth bag type dust remover, a pulse ultraviolet activation-microorganism degradation reactor, a smoke-gas-water washing purification tower, and an induced draft fan. The system creatively utilizes the suitability and the bioactivity degradation function of a Verticillium lecanii KM9083 strain to high-concentration VOCs, VOCs elements in the waste gas are finally decomposed into H2O and CO2, and the biological degradation efficiency of the strain to the VOCs can meet purification treatment requirements of the industrial waste gas. Meanwhile, the system utilizes an ultraviolet radiation resistance function of a benzophenone-hexamethyl phosphorotriamides combination, and adds the medicament to the Verticillium lecanii KM9083 strain in advance, thereby effectively preventing a biological inhibition function of ultraviolet rays to the strain.

The invention discloses an insecticide composition for preventing and controlling injurious insects such as mealybugs. Active ingredients of the insecticide composition comprise spirotetramat and verticillium lecanii, wherein the mass percent of the spirotetramat and the verticillium lecanii in the insecticide composition is 1-45%; and the mass ratio of the spirotetramat to the verticillium lecanii is (0.1-40): 1. The insecticide composition disclosed by the invention is mainly used for the prevention and control of injurious insects such as mealybugs, has the advantages of being synergistic, good in permeability, high in activity, stable in effect, long in persistent effect, small in environmental influences, strong in stickiness and evaporation-resistant, can be used for ultralow-volume plane sprays to control a plurality of crop insects at the same time, and has good application and popularization values.

The invention discloses a compound desinsection primary agent and pesticide consisting of verticillium lecanii and imidacloprid. The primary agent is a mixture of the verticillium lecanii and the imidacloprid, wherein, the mass ratio between the verticillium lecanii and the imidacloprid is 100: 5-50; while the pesticide consists of a primary agent and a secondary agent, according to mass percent, the primary main agent accounts for 5-50%, and the secondary agent 50-95%. Through long-term infection biological study and interior biological assay, the verticillium lecanii has strong infection desinsection effect on a plurality of pests, the sucking pest in particular. The compound pesticide consisting of verticillium lecanii and imidacloprid of the invention has good effect in preventing and controlling pests such as white fly, plant louse, diamond back moth and the like.

The invention discloses a microbial pesticide for preventing and treating fungal diseases. The microbial pesticide comprises the following materials in parts by weight: 22-40 parts of verticillium lecanii, 5-17 parts of corn flour, 11-20 parts of polyisobutylene succinimide, 25-36 parts of malic acid, 15-32 parts of diatomite, 3-7 parts of metarhizium powder, 15-30 parts of trichoderma harzianum, 5-19 parts of amino-oligosaccharin, 6-9 parts of beta-cypermethrin, 45-62 parts of streptomyces diastatochromogenes D and 15-18 parts of arteannuin. The microbial pesticide is applied to preventing and controlling fungal diseases of plants and is similar with or slightly superior to general chemical agents in effect, and a new way is provided for the development of agricultural fungicides.

The present invention discloses an insect disinfestation main agent compounded by verticillum lecanii and acetamiprid and an insecticide thereof. The main agent is the mixture of verticillum lecanii and acetamiprid. The mass ratio between verticillum lecanii and acetamiprid is 100: 1.5-12. The insecticide is composed of the main agent and assistant agent. According to the weight percentage, the main agent is 22-61%, and the assistant agent is 39%-78%. After long-time infection biology research and indoor biological assay, the verticillum lecanii has strong infection insect killing effect to a plurality of insects, especially the piercing-sucking mouthparts insects. The insecticide compounded by verticillum lecanii and acetamiprid has excellent control efficiency to insects of whitefly, aphids, diamondback moth, etc.

The invention discloses a predatory mite and verticillum lecanii combined-application biopesticide and a preparing and application method thereof and belongs to the field of plant insect pest prevention. The predatory mite and verticillum lecanii combined-application biopesticide is prepared by wheat bran, predatory mites and verticillum lecanii conidial powder in a mixed mode. According to the ratio, each kilogram of wheat bran is mixed with 1.5 * 105-2.0 * 105 predatory mites and 3-5 grams of verticillum lecanii conidial powder. The preparing method comprises the steps that 1. materials are prepared, wherein the verticillum lecanii conidial powder and the predatory mites are produced; and 2. the predatory mites and the verticillum lecanii conidial powder are mixed. The application method comprises the steps that on March to April or September to October each year, the combined biopesticide is applied in a broadcasting or powder spraying mode, so that the pesticide is evenly distributed on leaves, according to the using amount, 8-12 grams are sprayed to fruit trees at least 5 years old, 4-6 grams are sprayed to fruit trees at most 5 years old, and 450-550 grams are sprayed to each acre of vegetable and flowers.

The invention discloses a preparation method of a yield-increasing disease-resistant biological bacterial fertilizer for field crops. The preparation method comprises the following steps: step 1, preparing a first complex microbial inoculant and a second complex microbial inoculant; step 2, screening a plurality of strains from a strain library of verticillium lecanii, beauveria bassiana and metarhizium anisopliae, selecting three fungal strains which have the smallest antagonism with the second complex microbial inoculant obtained in the step 1 from the strains, and carrying out enlarged culture; step 3, preparing a coating agent; step 4, fermenting; step 5, granulating and coating; and step 6, carrying out low-temperature drying in vacuum. The problems that in the prior art, a field biological bacterial fertilizer prepared from plant ash, furfural residues and mushroom dregs does not contain various active microorganisms with different functions, the colonization survival performance of the microorganisms is low, and the disease prevention and insect prevention effects are not achieved can be solved.

A verticillium plasminogen is a fermentation liquid to be salted out and deposited to be desalinated by dialysis then to be chromatographed by DEAE and active peaks are collected to get rough enzymes after frozen and dried to be prepared with electrophoresis to be washed and dewateres to and get pare verticillium plasminogen with the molecular weight of 33KD, isoelectric point of 8.5 which has the fibrinolysis activation directly degradating fibrin and fine thrombus solving action.

The invention relates to a pair of specific primers for amplifying Verticillium lecanii. The invention solves the problem of lack of primers for amplifying the Verticillium lecanii target sequence from a sample DNA. The specific primers for amplifying Verticillium lecanii are (5'-CGGCGTCCGGACGCGGACCCAG-3' and VR(5'-CCCCAACGCCGACTTCCCCGAG-3'). The primers provided by the invention can accurately amplify the target segment of Verticillium lecanii.

The invention discloses verticillium lecanii LL-01 and a biocontrol application thereof, and belongs to the technical field of microorganisms. The preservation number of the strain is CGMCC No.21940. Under conditions of 26 DEG C optimal to growth and spore production, photoperiod of 6L: 18D, fructose as a carbon source and casein as a nitrogen source, the verticillium lecanii LL-01 has high growth speed, high spore yield, and accumulative fatality rates on planococcus lilacinus and phenacoccus solani ferris at different instars up to 84.09%-97.62% and 89.89%-98.85% respectively. The verticillium lecanii LL-01 has good biocontrol potential, can be used for replacing part of chemical pesticides for preventing and controlling mealybugs so as to reduce usage of chemical pesticides, and facilitates to maintain ecological balance.

The invention relates to a special compound fertilizer for chenopodium quinoa willd and a preparation method thereof, belonging to the technical field of crop fertilizer. The special compound fertilizer for chenopodium quinoa willd consists of an inorganic component and an organic component, wherein the mass content of the inorganic component is 30-35%; the inorganic component consists of urea, monopotassium phosphate and calcium superphosphate in a mass ratio of 10:8.2:10; the organic component is prepared by taking agricultural waste, wheat straw, distillers' grains and gypsum as fermentation base materials and performing aerobic fermentation using zymophyte; and the zymophyte consists of aspergillus carbonarius, aspergillus flavus, aspergillus fumigatus, rhizopus oryzae, aspergillus subolivaceus and verticillium lecanii. In the special compound fertilizer for chenopodium quinoa willd provided by the invention, the viable count exceeds 46,000,000(cfu)/g, the organic content exceeds 29.8wt%, and the pH is 6.9-7.1; abundant N, P, K and Ca and various beneficial trace elements are contained; and the yield and quality of chenopodium quinoa willd can be remarkably improved, and the insect damage and disease damage are effectively reduced.