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A technology of Verticillium lecanii and insecticides, applied in the fields of application, biocide, biocide, etc., can solve the problems of slow effect and decline in market share
Inactive Publication Date: 2008-08-27
YUNNAN AGRICULTURAL UNIVERSITY
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Summary
Abstract
Description
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Problems solved by technology
Due to the slow effect of this fungal preparation product, it has certain requirements for the use environment; in the development process (especially in the application process of aphid control), it has been affected by the competitive pressure of special chemical pesticides such as pirimicarb, resulting in market share declined
Method used
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Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0073] Verticillium lecanii K1207 was moved to a test tube slant culture medium, the formula of the test tube slant culture medium was: potato 20%, glucose 1.7-2%, agar 1.4-1.5%, natural pH.
[0074] Cultivate at a constant temperature of 28°C for 3-4 days to obtain test tube species.
[0075] Inoculate the test tube species into a 500ml Erlenmeyer flask (200ml per bottle) liquid medium, the medium formula is: 1% peptone, 4% glucose, 0.5% yeast extract; 0.136% KH 2 PO 4 , 0.12% MgSO 4 ·7H 2 O; pH 6.0-6.5, cultivated at 28°C for 4-5 days with constant temperature shaking, with a rotation speed of 100-120rpm, added 20ml / bottle into the secondary culture medium (improved SDAY), and cultured on a shaking table for 4-5d (100 ~120rpm) to obtain the liquid formulation of the present invention.
Embodiment 2
[0077] First obtain the liquid preparation (same as Example 1), after the liquid preparation is diluted 3 times with sterilized water, add solid culture medium in the ratio of 6:10, the formula of solid culture medium is wheat bran--grain bran culture medium: 56 % wheat bran, 24% fine bran, 20% chaff, 0.136% KH 2 PO 4 , 0.12% MgSO 4 ·7H 2 O, after mixing well, put it in a solid fermentation room fumigated and sterilized by peracetic acid, culture in the dark at 25±1°C for 6 days, and continuously light it for 3 days, then take it out and spread it on a glass plate in the fermentation room, 35±1°C, 100 files Air-dry at wind speed for 5 days. Obtain a solid preparation.
Embodiment 3
[0079] First carry out liquid-solid two-phase fermentation (same as Example 1 and Example 2), and then dry, pulverize, add a wetting agent, a filler and a stabilizer to mix the cultured product to make a wettable powder.
[0080] The preferred composition and ratio of each component of wettable powder are: spore powder (50~60%), wetting agent (sodium dodecylbenzenesulfonate, 2~6%), stabilizer (glycerin, 0.5%~1 %), filler (diatomaceous earth, 30-40%).
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Abstract
The invention relates to a verticillium lecanii pesticide as well as the application thereof, and belongs to the plant protection and pesticide technology field. The bacterial strain provided by the invention is verticillium lecanii K1207 with CGMCC No.2326. The invention produces the bacterial strain via liquid and solid two-phase fermentation, and is characterized in that a wheat bran and bran coat culture medium is adopted as the solid culture medium. The cultured product can be added with and mixed with a wetting agent, a bulking agent and a stabilizing agent to obtain wettable powder after being parched and being crumbled. The inventive verticillium lecanii bacterial strain has high virulence, thereby having good control effects on pests such as green peach aphids, urolcueon formosanums, acyrthosiphon dirhodums, cabbage aphids, cabbage worms, diamondback moths, and greenhouse whiteflies. The materials in fermentation process are cheap and have wide sources, which is suitable for industrialized production without environmental pollution.
Description
Technical field: [0001] The invention relates to the technical field of plant protection and pesticides, in particular to an insecticide for Verticillium lecanii and its application. Background technique: [0002] Verticillium lecanii (zimm) viegas is an entomopathogenic fungus of the class Hyphospora. The fungus was first discovered in the world by Neitner in 1861 on the Ceylon coffee wax scale (Lecanii coffeee). Regarding the basic research on the application of this fungus, Dr. Hall R.A. (1976-1985) of the British Greenhouse Crop Research Institute carried out earlier, and presided over the research on the commercialization of this fungal preparation in the early 1980s. Vertalec, the first V.lecanii aphidicide product developed by Hall since 1981 R Since its commercialization, more than 8 kinds of commodities in many countries have been produced and sold under the names of Vertalec, Mycotal, Thriptal, Vertisol, AgroBiocontrol Verticillium 50, Verticon, Verticillin, and ...
Claims
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Application Information
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