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Chip for gene detection of multiple vibrios at the same time, and detection and use thereof

A technology of co-inspection and chip, applied in the direction of microorganism-based method, measurement/inspection of microorganism, microorganism, etc.

Active Publication Date: 2009-07-08
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the application of gene chips for the simultaneous detection of Vibrio anguillarum, Vibrio harveyi, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio splendidus and Vibrio fischeri using this technology

Method used

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  • Chip for gene detection of multiple vibrios at the same time, and detection and use thereof
  • Chip for gene detection of multiple vibrios at the same time, and detection and use thereof
  • Chip for gene detection of multiple vibrios at the same time, and detection and use thereof

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Embodiment Construction

[0047] The gene chip of the present invention provides the 16S rRNA probe sequence corresponding to each Vibrio of Vibrio anguillarum, Vibrio harveii, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio splendidus and Vibrio fischeri, Heat shock protein hsp60 probe sequence, 16SrRNA forward primer sequence, 16S rRNA reverse primer sequence, heat shock protein hsp60 forward primer sequence, heat shock protein hsp60 reverse primer sequence, in addition, it also includes Vibrio anguillarum virulence gene virA virulence gene vah, virulence gene virC, virulence gene flaA, virulence gene empA and virulence gene angM, a total of 6 virulence genes; Vibrio harveyi virulence gene pap6 and virulence gene hemoluticus have a total of 2 virulence genes Vibrio alginolyticus virulence gene tlh and virulence gene ompK, a total of 2 virulence genes; Vibrio parahaemolyticus virulence gene trh, virulence gene tdh and virulence gene vppC, a total of 3 virulence genes; Vibrio virulence gene aerv,...

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Abstract

The present invention relates to a detection chip for performing gene detection to various vibrio and its detection and applications. The invention provides 16S rRNA sequences corresponding to each vibrio of vibrio anguillarum, vibrio harveyi, vibrio alginolyticus, vibrio parahaemolyticus, brilliant vibrio and Fisher vibrio; heat shock protein hsp60 probe sequence; virulence gene probe sequence; 16S rRNA forward primer sequence; 16S rRNA reverse primer sequence; heat shock protein hsp60 forward primer sequence; heat shock protein hsp60 reverse primer sequence; virulence gene forward primer sequence and virulence gene reverse primer sequence. The present invention has specific, sensitive and high-throughput features, can simultaneously detect six kinds of bacteria virulence genes, and the invention will effectively guide the production as an important disease early-warning detection method used in clinical diagnosis of aquatic animals.

Description

technical field [0001] The present invention relates to marine biological pathogen detection technology, specifically a method for detecting Vibrio anguillarum, Vibrio harveii, Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio splendidus and Vibrio splendiferus in aquatic biological bacterial isolates. A gene chip of vibrio DNA, and the detection of bacterial diseases by using the preparation method of the diagnostic gene chip. Background technique [0002] At present, there are many reports on bacterial detection methods. In addition to traditional physiological and biochemical detection combined with 16S rRNA sequencing, there are also immunological detection methods such as indirect fluorescent antibody technology, nucleic acid blot hybridization, and PCR detection. The traditional bacterial culture method and biochemical identification are cumbersome to operate and take several days to get the results. The immunological method fluorescent antibody technology only tak...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04C12R1/63
Inventor 黄倢杨冰戈蕾王秀华许拉刘莉宋晓玲
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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