20 results about "Vibrio harveyi" patented technology

The application discloses a Vibrio harveyi oral vaccine and a preparation method and application thereof. The application uses a lactococcus lactis NZ9000 expression system, modifies transformation parameters, effectively expresses Vibrio harveyi OmpU protein, constructs a lactococcus lactis (NZ9000) engineering bacterium expressing Vibrio harveyi outer membrane protein (OmpU), develops an oral vaccine against Vibrio harveyi infection, aims to reduce the infection of economic animals in seawater aquaculture by oral administration, provides a new scheme for dealing with the harm of Vibrio harveyi infection and antibiotic resistance and the like, promotes the breeding of fry and fingerlings, and promotes the development of biological agriculture and related industries. The strategy is expected to overcome the problems of traditional injection vaccines, such as inconvenient operation and great stress, and can provide a new technical scheme for reducing the dependence of the aquaculture industry on antibiotics and promoting the establishment of a green and healthy breeding mode.

The application discloses a freeze-drying protective agent of Vibrio harveyi phage V-YDF132 and a preservation method thereof. The freeze-drying protective agent contains inulin, skimmed milk powder, gelatin, L-glutamic acid sodium and water. The freeze-drying protective agent is mixed with a phage V-YDF132 solution, and then the mixture is frozen to a solid state, and after being subjected to freeze-drying, a phage V-YDF132 freeze-drying powder preparation is obtained. There are various preservation methods for phages, and the best preservation method is different for different phages. The application screens and prepares different phage freeze-drying protective agents and explores the protection effect of the freeze-drying protective agents on phages after freeze-drying, so as to lay a foundation for phage preservation. The phage powder preparation prepared from the freeze-drying protective agent formula P9 and the phage V-YDF132 has low requirements on the preservation conditions and can be long-term preserved without losing biological activity.

The invention provides an application of an Opnlrp12 gene in prevention and treatment of torus schlegeli virus and / or bacterial infection, and belongs to the technical field of gene engineering. Through targeted knockout of Opnlrp12, it is found that the expression level of a disease-resistant gene is also reduced, and therefore it is proved that Opnlrp12 can effectively inhibit infection caused by viruses and / or bacteria, and the survival rate of oplegnathus punctatus is increased. By means of the method, control over inflammatory diseases caused by iridovirus and / or vibrio harveyi can be achieved, and a target spot is provided for treatment of iridovirus infection of oplegnathus punctatus.

The invention provides a Babylonia areolata larva disease-resistant cultivation method based on immunostimulation, and aims to solve the problems of frequent larva diseases and low survival rate in intensive cultivation. The method comprises the following steps: selecting Babylonia areolata of Thailand and Hainan populations for reciprocal cross to obtain hybrid filial generations; in the larva stage, a compound immunologic stimulant compounded by astragalus polysaccharide, peptidoglycan and beta-glucan is adopted, the concentration is accurately regulated and controlled according to the early, middle and late stages of the daughter larvae and the creeping stage, and alternate induction is conducted through water splashing and bait mixing and feeding; when the larva shell length is 30-50mm, vibrio harveyi sublethal infection is used for enhancing immune memory, and environmental parameters such as water temperature and salinity are synchronously regulated and controlled. Tests show that the method can significantly improve the growth performance, immune enzyme activity and disease resistance of larvae, the survival rate of cultivation reaches 95% or above, the natural morbidity is lower than 6%, and technical support is provided for large-scale healthy cultivation of Babylonia areolata.

This invention discloses a method for cultivating pearl grouper, belonging to the field of aquaculture technology. Through careful design of active component configuration and juvenile feeding according to size, component A includes the following raw materials: β-chitosan, oxalic acid, and *Gnaphalium affine* extract; component B includes the following raw materials: sugarcane extract, rye seed powder, and oryzanol; component C includes the following raw materials: fig polysaccharide, yeast β-glucan, and eucalyptus oil. When the juvenile size is 2.2~5.0cm, a basic feed supplemented with components A and B is provided; when the juvenile size is 5.1~10cm, a basic feed supplemented with components A and C is provided; when the juvenile size is greater than 10cm, a basic feed supplemented with components A, B, and C is provided. The cultivation method of this application can improve the resistance of pearl grouper to *Vibrio harveyi* infection and / or its low-temperature tolerance, and the cultivation method is simple and the raw materials are readily available.

The invention discloses application of a gentiana macrophylla extract in preparation of a vibrio harveyi inhibitor. The gentiana macrophylla extract is obtained by extracting a gentiana macrophylla sample with an organic solvent. The gentiana macrophylla extract prepared by the invention can specifically inhibit vibrio harveyi, does not generate drug resistance, and has the characteristics of high efficiency, safety and no toxic or side effect. The invention also provides an aquaculture feed prepared from the gentiana macrophylla extract, and when aquatic animals are fed with the aquaculture feed disclosed by the invention, the vibrio harveyi resistance of the aquatic animals can be improved, and healthy, stable and sustainable development of the aquaculture industry is facilitated.

The invention provides lactobacillus plantarum XD1-2 as well as application and feed thereof, and belongs to the technical field of microorganisms. The lactobacillus plantarum XD1-2 disclosed by the invention is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No. 63902. The lactobacillus plantarum XD1-2 has relatively strong acid production activity, can secrete various organic acids, and has a relatively strong in-vitro bacteriostatic effect on aeromonas hydrophila, vibrio harveyi and escherichia coli; the lactobacillus plantarum XD1-2 is added into feed as a probiotic additive of cherax quadricarinatus feed, the digestion function, immunity and disease resistance to pathogenic bacteria of cherax quadricarinatus can be improved, and the lactobacillus plantarum XD1-2 has great value in development and application of green feed additives of cherax quadricarinatus.

The present application relates to the technical field of genetic engineering, in particular to two Cynoglossus semilaevis anti-vibrio disease genes and application methods. The present application mainly studies the role of CD79a and CD79b in Cynoglossus semilaevis in resisting vibrio disease, and verifies from two directions of in vivo and in vitro, further promotes the progress of Cynoglossus semilaevis disease resistance breeding, and shows that CD79a and CD79b have the role of inhibiting Vibrio harveyi.

The present application relates to the field of microbial molecular diagnosis, and particularly relates to a detection platform capable of activating Cas12a transcleavage activity without PAM sequence and application thereof in rapid detection of waterborne pathogenic bacteria Vibrio harveyi. The present application breaks through the PAM dependence restriction, realizes efficient activation of Cas12a without PAM sequence by optimizing the composition of dsDNA target positioning and crRNA spacer region, and expands the target selection range of the CRISPR-Cas12a detection system without converting dsDNA into ssDNA. The present application simplifies the detection process, eliminates the pretreatment steps such as heat denaturation and enzymolysis, reduces the types of reagents and operation steps, reduces the risk of sample contamination, shortens the total detection time, and is more suitable for on-site rapid detection. The present application has high clinical application value. In the detection of clinical samples of infected Trachinotus ovatus, the detection results of the PFCD platform are completely consistent with those of qPCR (the existing molecular diagnosis "gold standard"), and the infected individuals can be accurately identified, thereby providing reliable technical support for early diagnosis and prevention and control of waterborne diseases.

The application provides a constraint brown rod bacterium isolated from the intestine of Cephalopholis leopardus and an application thereof, and the preservation number is CGMCC No. 36569. The strain of the application has significant antagonistic effect on Vibrio harveyi, and can be used for preparing products for preventing and controlling aquatic Vibrio diseases. The strain is derived from fish intestine, has good environmental adaptability and host colonization capacity, is high in safety, has no hemolytic activity and carries no drug resistance gene. Feeding experiments show that the strain can promote fish growth, improve feed utilization rate, and enhance the immunity of the body. The application also provides an application of the strain in feed additives or bacterial liquid products, and the application has good popularization value.

The present application relates to oligosaccharide composition and its use. The present application particularly relates to the use of oligosaccharide composition in the preparation of products for preventing and / or treating aquaculture diseases caused by Vibrio parahaemolyticus, Vibrio harveyi and Streptococcus agalactiae, wherein the oligosaccharide composition is obtained by degrading polygalacturonan or pectin by a polygalacturonase as shown in SEQ ID No: 1, under the conditions of a polygalacturonase concentration of 0.045 mg / mL to 0.055 mg / mL, a polygalacturonan concentration of 7% to 9%, and a degradation time of 12 hours to 20 hours. The present application also relates to the use of oligosaccharide composition in aquaculture. The oligosaccharide composition of the present application has good bacteriostatic effect and disease prevention and control effect in aquaculture.

The invention discloses an application of a phage vBVhaMR41S in prevention and control of aquaculture source vibrio harveyi. The vibrio harveyi phage vBVhaMR41S is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on December 8, 2025, the address is No.100 courtyard of the middle road of Xianlie in the Visu District of Guangzhou, Guangdong province, the postcode is 510070, and the preservation number is GDMCC NO. GDMCC 67437-B1. The vibrio harveyi phage vBVhaMR41S has the advantages that the vibrio harveyi phage vBVhaMR41S is named as Vibrio harveyi phage vBVhaMR41S; the bacteriophage vBVhaMR41S shows extremely high host specificity, and only a specific pathogenic strain is split without influencing other environmental flora. The bacteriophage vBVhaMR41S has the advantages of strong environmental adaptability, high bactericidal efficacy and good biological safety, and is an ideal candidate preparation for preventing and controlling vibrio harveyi infection in aquaculture.

The invention provides an active polypeptide and application thereof in culture of tegillarca granosa, and after amino acid optimization, the provided active polypeptide has a remarkable inhibition effect on common microorganisms such as vibrio and the like in aquaculture. The active polypeptide is further prepared into microcapsules with the particle size suitable for filter feeding of juvenile tegillarca granosa, and the microcapsules are fed to the tegillarca granosa, so that microbial diseases occurring in tegillarca granosa breeding are effectively reduced. The amino acid sequence of the polypeptide is SEQ ID NO: 2. The active polypeptide obtained by screening comes from pearl oyster, the amino acid sequence of the provided active polypeptide is optimized, and the active polypeptide has good antibacterial activity on vibrio harveyi. After high expression of the active polypeptide, the active polypeptide is prepared into the microcapsule with the particle size suitable for filter feeding of juvenile shellfish of tegillarca granosa, the growth of the tegillarca granosa is promoted, and diseases caused by vibrio harveyi are reduced.

This invention relates to a fish interferon regulator IRF4 gene, protein, expression vector, and their applications, belonging to the field of fish molecular immunology technology. The fish interferon regulator IRF4 is derived from the oval pomfret, and the cDNA nucleotide sequence of the oval pomfret IRF4 gene is shown in SEQ ID NO.1. The amino acid sequence of the protein encoded by the oval pomfret IRF4 gene is shown in SEQ ID NO.2. This invention also provides an expression vector containing the oval pomfret IRF4 gene, and an anti-Vibrio harveyi infection preparation containing the protein or a eukaryotic expression vector. The oval pomfret IRF4 protein of this invention can promote the respiratory burst activity and phagocytic capacity of oval pomfret head kidney macrophages. Overexpression in oval pomfret... Believe After IRF4 is activated, the body's antibacterial ability can be significantly improved, and it can be used as an immune enhancer.

The application provides a composition for rapid detection of grouper iridovirus and Vibrio harveyi and application thereof. The composition comprises a first primer set and a first fluorescent probe for detecting the grouper iridovirus, and a second primer set and a second fluorescent probe for detecting the Vibrio harveyi, wherein the nucleotide sequences of the first primer set and the first fluorescent probe are shown as SEQ ID NO: 1-3, and the nucleotide sequences of the second primer set and the second fluorescent probe are shown as SEQ ID NO: 4-6. The composition provided by the application can realize rapid quantitative detection of SGIV and VH simultaneously through multiplex fluorescent quantitative PCR, and the interference between the primers and the probes is less, and the detection result is not affected, so that the detection result has the characteristics of high specificity, high sensitivity, stability and good repeatability. In addition, the SGIV and the VH can be subjected to nucleic acid amplification in the same reaction system, the detection time is greatly shortened compared with ordinary PCR in which amplification is followed by agarose gel electrophoresis analysis, the detection efficiency is improved, and the detection cost is lower than that of single fluorescent quantitative PCR.

The present application discloses a dark striped pufferfish L-type agglutinin VIP36, the amino acid sequence of which is shown as SEQ ID NO. 1, and the coding gene of which is shown as SEQ ID NO. 2. The present application also discloses the application of the dark striped pufferfish L-type agglutinin VIP36 in the preparation of a preparation with the efficacy of inhibiting Staphylococcus aureus, Vibrio parahaemolyticus, Aeromonas hydrophila and Vibrio harveyi. The present application firstly clones the coding gene of the dark striped pufferfish L-type agglutinin VIP36, constructs a prokaryotic expression vector of the dark striped pufferfish L-type agglutinin VIP36 and develops a recombinant protein; studies the use of the VIP36 recombinant protein in the immune defense function of the dark striped pufferfish, and the immune defense function includes regulating the homeostasis and health of the body, and the present application provides a theoretical basis for preventing diseases caused by pathogenic bacteria and a theoretical guidance for the healthy breeding of the dark striped pufferfish.

The application discloses a nucleic acid aptamer of Aeromonas hydrophila and a screening method and application thereof. The nucleic acid aptamer is obtained by SELEX technology, and the affinity of the nucleic acid aptamer to the target bacteria Aeromonas hydrophila is significantly higher than that to non-target bacteria (Vibrio anguillarum, Edwardsiella tarda, Pseudomonas mutabilis and Vibrio harveyi) (P<0.05). On this basis, a nucleic acid aptamer-qPCR quantitative detection method for the bacteria is established, and the method has a good linear relationship in the range of 10-10 8 CFU / mL, a linear fitting coefficient R2=0.9904, and a minimum detection limit of 10 CFU / mL, and the nucleic acid aptamer has good detection effect in seawater and tissue samples.

This invention discloses a broad-spectrum short-tailed bacteriophage, PhLNJZ2, targeting multidrug-resistant Vibrio and its applications, belonging to the field of microbial technology. The bacteriophage PhLNJZ2 was deposited on October 20, 2025, at the China Center for Type Culture Collection (CCTCC), Wuhan, China, with accession number CCTCC NO:M 20252251, and classified as follows: Vibrio Phage PhLNJZ2. This bacteriophage was screened from a clinical isolate of a multidrug-resistant Vibrio alginolyticus resistant to more than seven antibiotics. It exhibits highly efficient and broad-spectrum lytic activity against three major aquatic pathogenic Vibrio species: Vibrio alginolyticus, Vibrio parahaemolyticus, and Vibrio harveyi, with a total lysis rate of 81.25%. This invention also provides compositions containing this bacteriophage, feed additives, and their application in the preparation of drugs or environmental disinfectants for the prevention and control of multidrug-resistant and mixed vibrio infections in aquatic organisms.

The invention relates to a gene of a humpback perch interferon regulatory factor IRF1 and application of the gene as an immunologic adjuvant, and belongs to the field of molecular biology technology and application, the sequence of the humpback perch IRF1 gene is as shown in SEQ ID NO.1, and the amino acid sequence of protein coded by the gene is as shown in SEQ ID NO.2. The eukaryotic expression plasmid of the humpback perch IRF1 constructed by the invention can be used as an immunologic adjuvant of the DNA vaccine pCTssj to improve the immune effect of the humpback perch IRF1 against vibrio harveyi infection, can be developed into an immune preparation, and has good practical application value.

The invention discloses a Lactococcus gasseri HN1 strain and an application of the Lactococcus gasseri HN1 strain. The Lactococcus garviae HN1 strain disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 67511. The HN1 strain can resist acid and bile salt and is good in safety, supernate of the HN1 strain has an obvious bacteriostatic effect on aquatic / zoonosis pathogenic bacteria such as vibrio harveyi, escherichia coli, vibrio anguillarum, aeromonas hydrophila, staphylococcus aureus, vibrio parahaemolyticus and edwardsiella tarda, and the HN1 strain can be prepared into antibacterial drugs and used for preventing and treating related diseases. Besides, the HN1 strain is very sensitive to ampicillin and tetracycline, is insensitive to antibacterial drugs such as rifampicin and the like, and also can be combined with insensitive or drug-resistant antibacterial drugs to better prevent and treat diseases caused by pathogenic bacterium infection.