111 results about "Vibrio harveyi" patented technology

The invention belongs to the technical field of aquatic pathogen diagnosis, and particularly relates to a Taqman multiple fluorescent quantitative PCR (Polymerase Chain Reaction) detection system and kit for common vibrio pathogens in aquaculture as well as application and a detection method. The system comprises primers and probes for detecting four common vibrio pathogens in aquaculture, the 5'end of each probe is modified with different fluorophores, the 3 'end of each probe is modified with corresponding quenching groups, and the common vibrio pathogens comprise vibrio anguillarum Va, vibrio alginolyticus Val, vibrio harveyi Vh and vibrio scophthalmi Vsc.

The invention belongs to the technical field of aquatic biotechnology and molecular marker-assisted breeding, and particularly relates to a cynoglossus semilaevis vibrio harveyi-resistant KASP molecular marker combination and application thereof in cynoglossus semilaevis disease-resistant breeding. The KASP molecular marker combination comprises three SNP (Single Nucleotide Polymorphism) loci which are respectively positioned at 17314099, 17325496 and 17355989 loci of the 14 # chromosome of the cynoglossus semilaevis, when the genotypes of the three SNP loci are respectively CC, AA and GG, the disease resistance of the cynoglossus semilaevis is high, and the invention also provides a primer for detecting the genotype polymorphism of the SNP loci. By utilizing the KASP molecular marker combination, the vibrio harveyi resistance genotype in a cynoglossus semilaevis breeding population can be quickly detected, and disease-resistant individuals are screened, so that the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved, and a new variety of the cynoglossus semilaevis with strong disease resistance can be quickly cultivated.

The invention relates to the technical field of biology, in particular to a compound microbial preparation or fertilizer for improving water quality of a mariculture pond. The composite microbial preparation comprises the following functional components in concentration: 10 < 6 >-10 < 8 > cfu / ml of bifidobacterium thermoactinomycetes, 10 < 7 >-10 < 9 > cfu / ml of rhodopseudomonas palustris, 0.03-0.06 mg / ml of dwarf vallisneria natans extract and 0.01-0.03 mg / ml of acorus calamus extract. The compound microbial preparation is clear in components and scientific in compatibility, and all the components are mutually beneficial, mutualistic and synergistic; on the premise of ensuring no pollution, no residue and no drug resistance, the content of ammonia nitrogen, phosphorus and organic pollutants in the water body of the mariculture pond can be effectively reduced; meanwhile, heavy metals, antibiotics and vibrio harveyi in the pond water body can be effectively removed, and an obvious improvement effect on the mariculture pond water body is achieved.

The invention belongs to the technical field of aquaculture biological breeding, and particularly relates to a KASP marker of a vibrio harveyi resistant gene jam2a of cynoglossus semilaevis and application of the KASP marker. The KASP markers are respectively positioned at 14: 17 279470, 14: 17 281640 and 14: 17 283306 sites of the 14 # chromosome of the cynoglossus semilaevis, the polymorphism of the sites is T / C, G / T and T / A, and when the genotypes of the three sites are respectively homozygous TT, homozygous GG and homozygous TT, the tested individual of the cynoglossus semilaevis is a disease-resistant individual. The KASP marker significantly associated with the jam2a gene provided by the invention can be used for molecular marker-assisted breeding of the vibriosis resistance character of the cynoglossus semilaevis, and provides important technical support for improving the breeding efficiency of disease resistance of the cynoglossus semilaevis.

The invention relates to the technical field of gene engineering, in particular to two cynoglossus semilaevis vibriosis-resistant genes and an application method thereof. According to the invention, the effects of the CD79a and the CD79b in the cynoglossus semilaevis in resisting vibriosis are mainly studied, verification is respectively carried out in vivo and in vitro, the process of disease-resistant breeding of the cynoglossus semilaevis is further promoted, and it is indicated that the CD79a and the CD79b have the effect of inhibiting vibrio harveyi.

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for prawn culture pathogenic bacteria, which is characterized in that the kit contains primers and probes for detecting the prawn culture pathogenic bacteria, wherein the primers and the probes are respectively used for detecting TDH-related hemolsin genes of vibrio parahaemolyticus, ORF107 genes of white spot syndrome viruses, small subunit ribosomal genes of shrimp enterocytozoon hepatopenaei, 37 kDa coat protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA (deoxyribonucleic acid) primer genes of full-eye iridovirus 1 and PirA [pi] genes of pathogens of bacterial prawns acute hepatopancreas necrosis; the method has the advantage that the detection of eight prawn culture pathogens can be realized at the same time. The method comprises the following steps of: obtaining a tcdB gene of a high-pathogenicity vibrio and a hemolsin gene of a vibrio harveyi;

The invention relates to a multiplex PCR (polymerase chain reaction) primer group for simultaneously detecting four pathogens, a detection method and application, and belongs to the field of molecular biology, the four pathogens are nervous necrosis virus, iridovirus, streptococcus iniae and vibrio harveyi, and the primer group is composed of SEQ ID NO.1-8. By utilizing the primer, nervous necrosis virus, iridovirus, streptococcus iniae and vibrio harveyi can be simultaneously detected in a short time, and the aim of establishing a simple and effective method for simultaneously detecting various fish pathogens is fulfilled. And the detection primer group and the detection method provided by the invention have good specificity and relatively high sensitivity.

The invention belongs to the field of microorganisms, and particularly relates to a high-temperature-resistant vibrio harveyi bacteriophage, a composition thereof, a shrimp feed and application. The high-temperature-resistant vibrio harveyi bacteriophage Ph037 can play a very good cracking effect on vibrio harveyi, has very strong tolerance to high temperature, can be stably stored in transportation and preservation processes in different environments, solves the problem that the bacteriophage is easy to inactivate in a high-temperature environment, provides a new means for treating, preventing and controlling vibriosis, and has good application prospects. And an alternative scheme is provided for treatment of multi-drug-resistant bacterial infection.

The invention belongs to the technical field of molecular marker-assisted breeding of aquatic animals, and particularly relates to a KASP marker combination for detecting vibrio harveyi resistance of cynoglossus semilaevis and application of the KASP marker combination. The KASP marker combination disclosed by the invention is respectively positioned at the 14: 17 304461 site, the 14: 17 307045 site, the 14: 17 310738 site and the 14: 17 303501 site of the 14 # chromosome of the cynoglossus semilaevis, and the base polymorphism types of the KASP marker combination are C / T, C / G, C / T and A / G respectively. According to the invention, after genotyping is carried out on a to-be-detected individual, the to-be-detected individual is scored according to the vibrio harveyi disease resistance excellent and inferior genotypes, so that the vibrio harveyi disease resistance of the to-be-detected individual is measured; by utilizing the KASP molecular marker combination disclosed by the invention, the vibrio harveyi resistance of the cynoglossus semilaevis can be detected at low cost, high efficiency and high accuracy, and the disease-resistant breeding efficiency of the cynoglossus semilaevis is greatly improved.

The invention discloses a bacteriostatic Weissella fusiformis LCOR4 strain and an application of the bacteriostatic Weissella fusiformis LCOR4 strain. The bacteriostatic Weissella fusiformis LCOR4 strain is separated from marine sludge in an intertidal zone of a mangrove forest area and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC NO: 64982. The LCOR4 strain can effectively inhibit the growth of aquatic animal pathogenic bacteria such as vibrio anguillarum, vibrio harveyi, vibrio parahaemolyticus, aeromonas hydrophila and edwardsiella tarda, and can be prepared into an antibacterial agent or a microbial preparation, and the antibacterial activity of the LCOR4 strain is utilized to prevent and treat diseases caused by pathogenic bacteria infection. Besides, the LCOR4 strain is insensitive to antibacterial drugs such as enrofloxacin, neomycin, norfloxacin and rifampicin, and the strain can be combined with the insensitive or drug-resistant antibacterial drugs, so that the antibacterial effect is enhanced, diseases caused by pathogenic bacterium infection are better prevented and treated, and the long-term, stable and healthy development of the aquaculture industry is facilitated.

The invention discloses bacillus velezensis Bve1910, which is preserved in the China Center for Type Culture Collection (CCTCC) and has the preservation number of CCTCC NO. M 20221965. The strain and metabolites thereof have strong antagonistic effects on more than ten types of marine culture animal pathogenic bacteria such as vibrio harveyi, vibrio erwiniae, vibrio canbayi, vibrio parahaemolyticus, vibrio alginolyticus, vibrio anguillarum, vibrio cholerae, vibrio shewanii, vibrio rotifer, pseudoalteromonas and photobacterium damarae, are strong in antibacterial activity and wide in antibacterial spectrum, and can be used for preparing marine culture animal pathogenic bacteria. According to the preparation method, the traditional Chinese medicine composition is simple in preparation method and rich and stable in metabolite, an ammonium sulfate precipitation extract, a hydrochloric acid precipitation methanol extraction extract, an ethyl acetate extraction extract and a chloroform extraction extract all have a broad-spectrum bacteriostatic effect, functional substances playing the broad-spectrum antagonistic bacteriostatic effect are compounded and diversified, and the directional selection effect on bacteria in the environment and ecology is not generated; and pathogenic bacteria are not easy to generate tolerance, so that the method has good application safety and ecological safety properties.

The invention belongs to the technical field of disinfectant use evaluation, and particularly relates to an evaluation method for safe use of an aquatic disinfectant. The harmful bacteria (aeromonas hydrophila, vibrio harveyi, aeromonas veronii and nocardia) and beneficial bacteria are experimented, the harmful bacteria are used for exploring the concentration of the disinfectant, and the beneficial bacteria assist in verifying the reasonability of the concentration of the disinfectant, so that a proper disinfectant concentration range is obtained. The killing effect and the killing concentration of common pathogenic bacteria in aquaculture are explored through a common aquatic product disinfectant, meanwhile, the production concentration in the actual ecological environment is screened, and the appropriate and recommended use concentration of the aquatic product disinfectant is obtained. The method plays a scientific reference role in guiding reasonable use of the disinfectant and avoiding propagation of multiple drug-resistant bacteria.

The application discloses a Vibrio harveyi oral vaccine and a preparation method and application thereof. The application uses a lactococcus lactis NZ9000 expression system, modifies transformation parameters, effectively expresses Vibrio harveyi OmpU protein, constructs a lactococcus lactis (NZ9000) engineering bacterium expressing Vibrio harveyi outer membrane protein (OmpU), develops an oral vaccine against Vibrio harveyi infection, aims to reduce the infection of economic animals in seawater aquaculture by oral administration, provides a new scheme for dealing with the harm of Vibrio harveyi infection and antibiotic resistance and the like, promotes the breeding of fry and fingerlings, and promotes the development of biological agriculture and related industries. The strategy is expected to overcome the problems of traditional injection vaccines, such as inconvenient operation and great stress, and can provide a new technical scheme for reducing the dependence of the aquaculture industry on antibiotics and promoting the establishment of a green and healthy breeding mode.

The invention provides a primer combination for a multiple detection kit for marine pathogenic bacteria and the multiple detection kit. The primer combination comprises primer pairs and probes which are used for detecting 13 marine pathogenic bacteria: vibrio alginolyticus, vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio marine fish, vibrio river, vibrio menivibrio, vibrio mimicus, vibrio harveyi, vibrio occultus, vibrio horii, plesiomonas and aeromonas, and the sequence of the primer combination is as shown in SEQ1-SEQ39. According to the present invention, the genomic DNA of 13 marine pathogenic bacteria is identified and detected in one digital PCR reaction through the ultra-multiple digital PCR fluorescence coding technology, and the kit not only covers the common vibrio species such as vibrio cholerae, vibrio vulnificus, vibrio parahaemolyticus and the like, but also can identify the sea area vibrio, vibrio holii, vibrio occultus and the like, and the kit has good sensitivity, specificity and accuracy.

The invention provides a rapid drug sensitivity detection kit for a breeding site, and belongs to the technical field of drug sensitivity detection. By preparing the penaeus vannamei polypeptide and adding the penaeus vannamei polypeptide into the casein tryptone liquid culture medium, the penaeus vannamei polypeptide has a remarkable promotion effect on the growth of common vibrio parahaemolyticus, vibrio harveyi and edwardsiella tarda in aquaculture, and the bacterial culture time can be shortened. The penaeus vannamei polypeptide is added into a drug sensitivity detection solution to be matched with a triphenyltetrazole chloride color developing agent and a drug sensitivity piece detection system, the prepared rapid drug sensitivity detection kit is used for drug sensitivity detection of clinical diseased prawn samples, and the drug sensitivity detection result can be judged only within 5 hours. The result judgment time is 4 hours earlier than that of a common drug sensitivity detection kit without the prawn polypeptide, and the problem of on-site rapid drug sensitivity detection of an aquaculture farm can be solved.

The invention belongs to the technical field of gene engineering, and discloses bacteriophage lysozyme as well as a preparation method and application thereof. The bacteriophage lysozyme provided by the invention comprises an amino acid fragment with a sequence as shown in SEQ ID NO: 4 or a variant fragment with at least 80% homology with the fragment as shown in SEQ ID NO: 4. The antibacterial spectrum of the bacteriophage lysozyme covers a plurality of bacteria such as staphylococcus aureus, bacillus subtilis, escherichia coli, pseudomonas putida, vibrio alginolyticus, vibrio harveyi and vibrio parahaemolyticus, and the bacteriophage lysozyme has the advantages of excellent antibacterial performance, high thermal stability and high acid and alkali tolerance, and can be used for preparing the antibacterial bacteriophage lysozyme. The antibacterial agent can be well applied to the fields of food, aquaculture, animal husbandry and the like.

The application discloses a freeze-drying protective agent of Vibrio harveyi phage V-YDF132 and a preservation method thereof. The freeze-drying protective agent contains inulin, skimmed milk powder, gelatin, L-glutamic acid sodium and water. The freeze-drying protective agent is mixed with a phage V-YDF132 solution, and then the mixture is frozen to a solid state, and after being subjected to freeze-drying, a phage V-YDF132 freeze-drying powder preparation is obtained. There are various preservation methods for phages, and the best preservation method is different for different phages. The application screens and prepares different phage freeze-drying protective agents and explores the protection effect of the freeze-drying protective agents on phages after freeze-drying, so as to lay a foundation for phage preservation. The phage powder preparation prepared from the freeze-drying protective agent formula P9 and the phage V-YDF132 has low requirements on the preservation conditions and can be long-term preserved without losing biological activity.

The invention provides an application of an Opnlrp12 gene in prevention and treatment of torus schlegeli virus and / or bacterial infection, and belongs to the technical field of gene engineering. Through targeted knockout of Opnlrp12, it is found that the expression level of a disease-resistant gene is also reduced, and therefore it is proved that Opnlrp12 can effectively inhibit infection caused by viruses and / or bacteria, and the survival rate of oplegnathus punctatus is increased. By means of the method, control over inflammatory diseases caused by iridovirus and / or vibrio harveyi can be achieved, and a target spot is provided for treatment of iridovirus infection of oplegnathus punctatus.

The invention discloses a primer probe combination for detecting various prawn pathogens and application of the primer probe combination. The primer probe combination comprises a first primer probe set for detecting vibrio harveyi, a second primer probe set for detecting infectious subcutaneous and hematopoietic necrosis viruses, a third primer probe set for detecting Taura syndrome viruses and a fourth primer probe set for detecting infectious muscle necrosis viruses. The primer probe combination has the characteristics of high sensitivity, good repeatability and strong specificity, and can be used for specifically detecting vibrio harveyi, infectious subcutaneous and hematopoietic necrosis viruses, Taura syndrome viruses and infectious muscle necrosis viruses.

The application discloses a broad-spectrum short tail phage PhLNJZ2 targeting multi-drug resistant Vibrio and an application thereof, and belongs to the technical field of microorganisms. The phage PhLNJZ2 is preserved in the China Center for Type Culture Collection on October 20, 2025, the preservation address is Wuhan, China, the preservation number is CCTCC NO: M 20252251, and the classification and naming is Vibrio phage PhLNJZ2. The phage is screened from a multi-drug resistant Vibrio alginolyticus clinical isolate which is resistant to more than 7 kinds of antibiotics, exhibits high efficient and broad-spectrum lytic activity on three main waterborne pathogenic Vibrio, i.e., Vibrio alginolyticus, Vibrio parahaemolyticus and Vibrio harveyi, and the total lytic rate reaches 81.25%. The application further provides a composition containing the phage, a feed additive and an application of the phage in preparation of a medicine for preventing and controlling waterborne multi-drug resistant and mixed Vibrio disease or an environmental disinfectant.

The invention discloses a combined fungicide and application thereof in preparation of a medicine for preventing and treating red bottom of babylonia lutosa in a seedling raising process. The combined fungicide comprises a spore fungicide and a bacteriophage; the spore microbial agent comprises bacillus subtilis, bacillus velezensis, bacillus licheniformis, bacillus pumilus, bacillus coagulans, bacillus megaterium and bacillus circulans; the bacteriophages comprise vibrio parahaemolyticus bacteriophage, vibrio harveyi bacteriophage, vibrio alginolyticus bacteriophage and bdellovibrio The combined fungicide can effectively restrain and solve the red bottom phenomenon appearing at the pond bottom in the babylonia lutosa seedling raising process, the babylonia lutosa seedling quantity can be remarkably increased, and the babylonia lutosa seedling raising success rate can be increased.

The application provides a bacteriophage for controlling Vibrio infection, and belongs to the field of veterinary technology. The application designs a recombinant tail chain protein rTC and synthesizes an expression gene of the recombinant tail chain protein rTC through amino acid sequence alignment and structure analysis of tail chain proteins of Vibrio phages. The rTC gene is electroporated into Vibrio parahaemolyticus isolated from diseased prawns, and the host bacteria are infected with PHxp1 bacteriophage isolated from seawater; after the rTC gene is recombined with the PHxp1 bacteriophage, the collected bacteriophage is screened with Vibrio alginolyticus to obtain two bacteriophages PHxp1-1 and PHxp1-2 capable of lysing Vibrio alginolyticus; the bacteriophage is screened with Vibrio harveyi to obtain one bacteriophage PHxp1-3 capable of lysing Vibrio harveyi. The host spectrum of the three recombinant bacteriophages is widened. The recombinant tail chain protein bacteriophage greatly reduces the death and growth stagnation of prawns caused by Vibrio infection in prawn farms, and improves the breeding benefit.

The invention discloses a method for inhibiting gene horizontal transfer based on vibrio harveyi fnr knockout, and relates to the fields of molecular biology and gene engineering. The method for inhibiting gene horizontal transfer disclosed by the invention comprises the following steps: introducing a target exogenous plasmid into a conjugational transfer donor bacterium and then conjugating with a recipient bacterium. The fnr gene of the recipient bacterium vibrio harveyi is knocked out, so that the efficiency of obtaining exogenous plasmids by the vibrio harveyi through conjugational transfer is reduced, and the method has the characteristic that the conjugational transfer efficiency is remarkably reduced. Compared with a wild type host cell, the efficiency of obtaining the plasmid pMMB207 in donor bacteria escherichia coli by the host cell with the fnr gene knocked out through conjugational transfer is reduced by 6.76 times. According to the method, the obtaining capacity of host cells on exogenous plasmids is greatly reduced, and the method has very important significance on resisting pathogenicity and drug resistance of microorganisms.

The invention provides a Babylonia areolata larva disease-resistant cultivation method based on immunostimulation, and aims to solve the problems of frequent larva diseases and low survival rate in intensive cultivation. The method comprises the following steps: selecting Babylonia areolata of Thailand and Hainan populations for reciprocal cross to obtain hybrid filial generations; in the larva stage, a compound immunologic stimulant compounded by astragalus polysaccharide, peptidoglycan and beta-glucan is adopted, the concentration is accurately regulated and controlled according to the early, middle and late stages of the daughter larvae and the creeping stage, and alternate induction is conducted through water splashing and bait mixing and feeding; when the larva shell length is 30-50mm, vibrio harveyi sublethal infection is used for enhancing immune memory, and environmental parameters such as water temperature and salinity are synchronously regulated and controlled. Tests show that the method can significantly improve the growth performance, immune enzyme activity and disease resistance of larvae, the survival rate of cultivation reaches 95% or above, the natural morbidity is lower than 6%, and technical support is provided for large-scale healthy cultivation of Babylonia areolata.

The invention discloses a method for inhibiting gene horizontal transfer based on vibrio harveyi tfoX knockout, and relates to the fields of molecular biology and gene engineering. The method for inhibiting gene horizontal transfer disclosed by the invention comprises the following steps: introducing a target exogenous plasmid into a conjugational transfer donor bacterium and then conjugating with a recipient bacterium. By knocking out the fX gene of the recipient bacterium vibrio harveyi, the efficiency of obtaining exogenous plasmids by the vibrio harveyi through conjugational transfer is reduced, and the method has the characteristic of remarkably reducing the conjugational transfer efficiency. Compared with a wild type host cell, the efficiency of obtaining the plasmid pMMB207 in donor bacteria escherichia coli through conjugational transfer by the tfoX gene knockout host cell is reduced by about 1000 times. According to the method, the obtaining capacity of host cells on exogenous plasmids is greatly reduced, and the method has very important significance on resisting pathogenicity and drug resistance of microorganisms.

The invention discloses a broad-spectrum antibacterial lactobacillus plantarum, and belongs to the technical field of microorganisms. The lactobacillus plantarum 9-6 is separated from animal feed, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32568, the lactobacillus plantarum 9-6 and fermentation liquor thereof have an obvious inhibition effect on common vibrio parahaemolyticus, vibrio alginolyticus, vibrio harveyi and vibrio vulnificus, and the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the advantages that the lactobacillus plantarum 9-6 and the fermentation liquor thereof have the obvious inhibition effect; and in addition, the antibacterial agent has a relatively good antibacterial effect on common staphylococcus aureus, escherichia coli, listeria monocytogenes, salmonella enterica and bacillus cereus. The lactobacillus plantarum 9-6 has probiotic safety, can grow under an initial pH condition of 4-10 and a high-salt environment, and plays a role in bacteriostasis under an acidic condition. The invention lays a foundation for developing a good antibacterial probiotic preparation and application of a fermentation culture thereof.

The application discloses a method for inhibiting horizontal gene transfer based on fnr knockout of Harveyi Vibrio, and relates to the fields of molecular biology and genetic engineering. The method for inhibiting horizontal gene transfer comprises the following steps: introducing a target exogenous plasmid into a conjugative transfer donor bacterium, and then conjugating with a receptor bacterium. The efficiency of the Harveyi Vibrio in obtaining the exogenous plasmid through conjugative transfer is reduced by knocking out the fnr gene of the receptor bacterium, and the method has the characteristics of significant reduction in the conjugative transfer efficiency. Compared with a wild-type host cell, the efficiency of the host cell with the fnr gene knocked out in obtaining the plasmid pMMB207 in the donor bacterium Escherichia coli through conjugative transfer is reduced by 6.76 times. The method greatly reduces the acquisition ability of the host cell to the exogenous plasmid, and has great significance in resisting microbial pathogenicity and drug resistance.

This invention discloses a method for cultivating pearl grouper, belonging to the field of aquaculture technology. Through careful design of active component configuration and juvenile feeding according to size, component A includes the following raw materials: β-chitosan, oxalic acid, and *Gnaphalium affine* extract; component B includes the following raw materials: sugarcane extract, rye seed powder, and oryzanol; component C includes the following raw materials: fig polysaccharide, yeast β-glucan, and eucalyptus oil. When the juvenile size is 2.2~5.0cm, a basic feed supplemented with components A and B is provided; when the juvenile size is 5.1~10cm, a basic feed supplemented with components A and C is provided; when the juvenile size is greater than 10cm, a basic feed supplemented with components A, B, and C is provided. The cultivation method of this application can improve the resistance of pearl grouper to *Vibrio harveyi* infection and / or its low-temperature tolerance, and the cultivation method is simple and the raw materials are readily available.

The application discloses a polypeptide and application thereof, and belongs to the technical field of peptide inhibitors. The polypeptide has an amino acid sequence as shown in SEQ ID NO:1; the polypeptide has a bacteriostatic and / or bactericidal function. The polypeptide can inhibit the growth of Vibrio parahaemolyticus at 128 muM, can inhibit the growth of Vibrio harveyi, Vibrio alginolyticus and Vibrio anguillarum at 256 muM, and can inhibit the growth of Aeromonas hydrophila at 512 muM, and can be used for preparing products with bacteriostatic and / or bactericidal properties, such as food or aquaculture products.

The invention relates to the technical field of Chinese herbal medicine screening and extraction and separation, in particular to application of a water chestnut hull extract, namely application of the water chestnut hull extract in preparation of drugs for inhibiting marine pathogenic bacteria. The marine pathogenic bacteria are Vibrio harveyi or Vibrio parahaemolyticus, and the marine pathogenic bacteria are the Vibrio harveyi or the Vibrio parahaemolyticus. The water chestnut hull extract is obtained by taking water chestnut hulls as raw materials and adopting water extraction or alcohol extraction. Compared with the prior art, the preparation method has the following beneficial effects that the water chestnut hull extract is used for preparing the medicine for inhibiting the marine pathogenic bacteria, the water chestnut hull alcohol extract has a relatively good inhibition effect on vibrio harveyi and vibrio parahaemolyticus, and the MIC values are 31.25 mg / mL and 15.63 mg / mL respectively.