274 results about "Arcobacter" patented technology

The invention discloses an application of bdellovibrios in removing pathogenic vibrio in marine food products and the culture water. The bdellovibrio concentrated solution is added into the marine food products and / or the culture water so as to lead the concentration of the bdellovibrio to reach at least 10<2>pfu / ml. When the invention is applied before eating the marine food products, in the transportation process and in the culture water, the concentration range of bdellovibrio is respectively 10<4>-10<12> pfu / ml, 10<3>-10<11> pfu / ml, and 10<2>-10<6 > pfu / ml. More than 90% pathogenic vibrio carried by marine food products before eating and / or in the transportation process is cleared by adopting a biological method, and the pathogenic vibrio in the culture water can also be controlled within10cfu / ml. The invention is suitable for the pretreatment process before being eaten or processed, the transportation process and the culture process of marine food products, especially facilitating the reduction or elimination of chemical medicine residues such as antibiotics, thus fundamentally avoiding the occurrence of food poisoning of the marine food products.

The invention discloses bacillus pumilus, a probiotics preparation and a preparation method and application thereof. The Bacillus pumilus LV149 is preserved in China center for type culture collection (CCTCC) in Nov. 23th, 2011, and the preservation number is CCTCC NO: M 2011411. The bacillus pumilus LV149 has strong extracellular protease, lipase and amylase activities, has wide rejection capability to vibrio and has no hemolytic activity. The Bacillus pumilus LV149 serves as fermenting bacterial strains and is performed with solid fermentation, drying and smashing so as to prepare bacillus pumilus probiotics preparation which uses Bacillus pumilus LV149 as the active ingredients. The bacillus pumilus probiotics preparation can be added into prawn feeds for feeding prawns, so that growth of prawn intestinal pathogenic vibrio can be restrained, prawn vibriosis can be reduced, simultaneously prawn growth is promoted, fish bait coefficient is reduced, quality of commodity is improved, culture cycle is shortened, accordingly culture risk and culture cost are reduced, biological safety is high and the bacillus pumilus, the probiotics preparation and the preparation method and application thereof have wide application prospect in aquaculture.

The invention relates to a Vibrio bacteriophage and a bactericidal composition preparation method and application thereof, belonging to the field of biotechnology. The bacteriophage composition comprises Vibrio alginolyticus bacteriophage vB_ValS_PcR-1 (accession number being CCTCC NO: M 2018391), Vibrio harveyi vB_VhaM_PcB-1G (accession number being CCTCC NO: M 2018392) and Vibrio parahaemolyticus phage vB_VhaP_OW (accession number being CCTCC NO: M 2015577). A high-potency culture product can be obtained after host actions, the composition is good in stability at room temperature, has a widehost splitting range, can efficiently inhibit growth of main pathogenic vibrios such as vibrio alginolyticus, vibrio harveyi, vibrio parahaemolyticus, can be applied as a biological antimicrobial agent for vibrio contamination control in food and aquaculture.

The invention discloses a microbial preparation for improving an aquatic product culture environment and a preparation method thereof. A strain with optimal nitrite degradation effects is screened and separated from a mud sample in Tongan and Haicang ports of Xiamen city of the Fujian province, another strain with optimal nitrite degradation effects is screened and separated from a mud sample in the intertidal zone of Ningde city of the Fujian province, and the two strains respectively are Rhodopseudomonas palustris PSB20 and Bacillus subtilis ND04. The microbial preparation prepared from the two strains can degrade ammonia nitrogen and nitrous acid in an aquatic product culture environment, has good decomposition effects on residual bait organic matters, has a certain effect of inhibiting pathogenic vibrio bacterium growth in culture water, and has a good production application prospect. The microbial preparation has the characteristic of simple preparation processes.

The invention discloses a vibrio qinghaiensis Q67 based long-term microplate toxicity analyzing method of environmental pollutants. The long-term microplate toxicity analyzing method is established by using the vibrio qinghaiensis Q67 as a tested organism and introducing time factors into the toxicity concentration-effect relationship of the traditional compound on the basis of a microplate toxicity analyzing method. The vibrio qinghaiensis Q67 based long-term microplate toxicity analyzing method of the environmental pollutants is characterized by adopting a specific microplate design and sampling scheme and a microplate culture medium suitable for the growth of the vibrio qinghaiensis Q67, measuring the long-term toxicity effect of the environmental pollutants on the vibrio qinghaiensis Q67 and repeatedly measures the toxicity of the same pollutant through multiple plates to ensure the statistical significance of an experimental result. By measuring the long-term toxicity of a part of the environmental pollutants on the vibrio qinghaiensis Q67, compared with short-term toxicity, the long-term toxicity of the tested environmental pollutants is discovered to be obviously higher than the short-term toxicity of the tested environmental pollutants, wherein the difference of the long-term toxicity and the short-term toxicity of antibiotics is most significant. The invention can actually reflect the toxicity of a site compound having specific action on compounds and more reasonably evaluate the toxicity of the pollutants.

The present invention relates to a detection chip for performing gene detection to various vibrio and its detection and applications. The invention provides 16S rRNA sequences corresponding to each vibrio of vibrio anguillarum, vibrio harveyi, vibrio alginolyticus, vibrio parahaemolyticus, brilliant vibrio and Fisher vibrio; heat shock protein hsp60 probe sequence; virulence gene probe sequence; 16S rRNA forward primer sequence; 16S rRNA reverse primer sequence; heat shock protein hsp60 forward primer sequence; heat shock protein hsp60 reverse primer sequence; virulence gene forward primer sequence and virulence gene reverse primer sequence. The present invention has specific, sensitive and high-throughput features, can simultaneously detect six kinds of bacteria virulence genes, and the invention will effectively guide the production as an important disease early-warning detection method used in clinical diagnosis of aquatic animals.

The invention provides bacillus subtilis (Bacillussubtilis) shou003, an anti-vibrio protein and a preparation method and applications of the bacillus subtilis shou003 and the anti-vibrio protein. The preparation method of the anti-vibrio protein comprises the following steps: screening out bacillus subtilis shou003 (preserved in China Center for Type Culture Collection with a number of CCTCC No.: M2013571 on November 13, 2013) from intestinal tract of a healthy large yellow croaker; and then extracting the anti-vibrio protein from the fermentation broth of bacillus subtilis shou003, wherein the amino acid sequence of the anti-vibrio protein is shown as SEQ ID No.: 1. The bacillus subtilis shou003 and the anti-vibrio protein show good effects on inhibiting aquatic pathogenic bacteria, in particular pathogenic vibrio; in addition, the bacillus subtilis shou003 shows outstanding tolerance to temperature, NaCl, gastric juice, intestinal juice and cholate and can be widely applied to the prevention of germs during aquaculture; on that basis, the optimal fermentation culture method of the bacillus subtilis shou003, and a preparation method of the anti-vibrio protein are provided.

The invention discloses a gene chip of aquatic product cultivation pathogenic bacterium, comprising a solid phase carrier which is modified chemically, a detection probe and a quality control probe are distributed on the solid phase carrier in a dot matrix way; the detection probe comprises specificity 16S rDNA sequences and / or gyrB gene sequences of vibrio, comma bacillus, vibrio harveyi, vibrio alginolyicus, vibrio anguillarum, vibrio parahemolyticus, nocardia, nacardia seriolea, aeromonas, hydrophilic aeromonas, streptococcus and dolphin streptococcus, which are to be detected, the quality control probe includes PCR positive, chip fixed positive control, chip hybridizing negative control, chip hybridizing positive control and chip hybridizing blank control; the gene chip has the advantages of small volume and high flux, can detect known and unknown germs of the vibrio, the nocardia, the aeromonas and the streptococcus, and can detect specific germs with multiple kinds, and the simpleness and rapidness and specificity of the germs can be detected, and automatic detection can be carried out after detection software is additionally arranged.

The invention relates to bifidobacterium longum subsp. infantis CCFM687 as well as fermented food and application thereof. The bifidobacterium longum subsp. infantis CCFM687 provided by the inventioncan improve the depressive behavior of a depression mouse and raise the level of 5-hydroxytryptamine, 5-hydroxytryptophane and brain-derived neurotrophic factor in the brain of the depression mouse; moreover, the content of butyric acid in the intestines of the depression mouse is increased; the abundance of intestinal desulfovibrio is lowered while the abundance of bifidobacterium and S24-7 family is raised, the intestinal flora alpha-diversity is improved, the intestinal flora disturbance of the depression mouse is relieved, and the occurrence of autism, inflammatory bowel disease, obesity,diabetes mellitus type I and the like is reduced; the level of mRNA simulating tryptophan hydroxylase in enterochromaffin cell is raised, the secretion of 5-hydroxytryptophane of the enterochromaffincell is increased, and a precursor substance is provided for the synthesis of 5-hydroxytryptamine in brain. Therefore, the bifidobacterium longum subsp. infantis CCFM687 has a broad application value.

The invention relates to a method for constructing a specific pathogen-free penaeid shrimp culture system, which comprises the steps of adopting comprehensive treatment of culture water for avoiding the entry of pathogens; introducing selected oocystis borgei into a penaeid shrimp culture pond for constructing a good microalgae community structure, and using a microalgae-controlled water environment to reduce the concentration of injurious factors, such as ammonia nitrogen, nitrite nitrogen and the like in a water body, improving the water quality, eliminating stress factors, improving the content of dissolved oxygen in the water and leading the environment of the water body to be in the good dynamic balance state for a long time. A system of the oocystis borgei, rhodopseudanonas palustris and phycomycete is utilized for inhibiting the growth of vibrios during the early stage, the middle stage and the late stage of culture; and schmackeria dubin is utilized to intake microalgae, thereby controlling the number of the microalgae in the water body and eliminating the adverse impacts of rapid proliferation of the microalgae on a culture environment. Penaeid shrimps have the advantages of specific pathogen-free property, rapid growth, strong disease resistance, strong stress resistance, short culture period, large and uniform formed size, high output, good investment efficiency and the like.

An isolated strain of bacteriophage, specific against bacteria belonging to the Vibrio genre, particularly the anguillarum species, deposited on 3 Oct. 2012 at the Polish Collection of Microorganisms (PCM) of the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy of the Polish Academy of Sciences, with access number F / 00072, characterized in that said strain is efficient in the prophylaxis, control and / or treatment of the infection caused by Vibrio anguillarum in all types of species of fish, mollusks and crustaceans that are important for aquaculture susceptible to this bacteria, genome size 48.6 Kb, it is not sensitive to chloroform and its storage temperature is −80° C.

The invention relates to a Vibrio FC509 strain and a culturing method and application thereof. The Vibrio FC509 strain is collected in the China General Microbiological Culture Collection Center (CGMCC) on 12, March, 2014, with a collection number of CGMCC NO.8913 and an address of Institute of Microbiology Chinese Academy of Sciences NO.1 West Beichen Road, Chaoyang District, Beijing. The Vibrio sp. FC509 strain obtained by first-time separation is capable of preparing glycosaminoglycan lyase which has a specific activity of 110,000U / mg to hyaluronic acid and specific activity of 45,000U / mg to chondroitin sulfate; enzyme activity is tens to hundreds times that of the known commercialized glycosaminoglycan lyase, can be applied to fields such as medicines, cosmetics and the like, and is wide in application prospect.

The invention discloses a vibrio-splendidus-resistant egg yolk antibody and a preparation method thereof. The vibrio-splendidus-resistant egg yolk antibody is obtained from a vibrio splendidus immunized laying hens, has features of good specificity and high titer, is used for preventing and treating diseases caused by vibrio splendidus and immunological detection, and has good effects. The vibrio-splendidus-resistant egg yolk antibody is obtained by extracting hyperimmune eggs laid by the vibrio splendidus immunized laying hens, blood sampling is not required, immunized animals are not injured, and modern animal rights protection rules are met; and each egg contains more than 100mg of the vibrio-splendidus-resistant egg yolk antibody approximately, and 3g of the vibrio-splendidus-resistant egg yolk antibody can be obtained in a month, and is 10-20 times greater than an antibody extracted from blood of an immunized rabbit. The vibrio-splendidus-resistant egg yolk antibody has the advantages of safety, environmental protection, high efficiency, low cost and the like, and is easy to industrialize.

The first primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of bacteria of the Escherichia, Salmonella and Vibrio genera, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs. The second primer of the invention is a primer which, when used in PCR under appropriate conditions, serves to detectably amplify 16S rRNA-encoding DNAs of Staphylococcus aureus and Bacillus cereus, but when used in PCR under the same conditions, does not serve to detectably amplify either chloroplast 16S rRNA-encoding DNAs or mitochondrial 16S rRNA-encoding DNAs.

The invention relates to the technical field of functional microbiological screening and provides novel Bacillus licheniformis DN29 which is collected under CCTCC NO: M2015482. The Bacillus licheniformis DN29 obtained by screening can effectively inhibit pathogenic bacteria such as Vibrio splendidus and pseudoalteromonas, can reduce disease probability of bred animals and can serve as a feed additive to remarkably increase feed utilization rate of the bred animals and promote growing of the animals. The Bacillus licheniformis DN29 can serve as a probiotic to be applied in the process of rearing and producing Stichopus japonicus, has remarkable promoting effect on growing of Stichopus japonicus and can effectively improve immunity of and resistance, to Vibrio splendidus, of Stichopus japonicus.

The present invention is preparation and usage of outer membrane protein subunit morbid vibrio vaccine for seawater fish. The vaccine is prepared through extracting two or more of Vibrio alginolyticus, Vibrio Hrveyi, Vibrio vulnificus, Vibrio parahaemolyticus, etc, and adding immunostimulating complex. The vaccine preparing process includes culturing vibrio, extracting outer membrane protein and preparing vaccine. The vaccine containing partial pathogen and no infecting component has stable immunological specificity and no hidden danger of restoring toxici. The vaccine is used in preventing fishes' skin ulcer, eyeball turbidity and other vibrio caused diseases and may be used widely for immunizing seawater fish.

The invention discloses a universal gene-knockout suicide vector for vibrios and a construction method theroef and provides an application thereof in gene knockout of the vibrios. The universal gene-knockout suicide vector pLP12 is a ring-shaped vector and comprises a PBAD promoter, a repressor protein gene araC, an RP4 transferring initiation site, a chlorampenicol resistant gene, an R6K duplicating initiation site, a multiple-cloning-site area and a lethal gene vmi480; the multiple-cloning-site area at least contains two AhdI restriction enzyme digestion sites; the suicide vector pLP12 is subject to AhdI restriction enzyme digestion to form linearized suicide vector pLP12T. The universal gene-knockout suicide vector adopts entirely-new reverse selection genes vmi480 and is used for replacing the common sacB gene. Foreign fragments carried by the pLP12T are transferred to vibrio cells to be mutated by a jointing mode, under the pressure of antibiotics and reverse selection of products of lethal gene vmi480, first-time homologous recombination and second-time homologous recombination are carried out on the vibrios successively, and finally the mutant strain with deletion of target genes is generated.

The invention relates to a method for enriching vibrio phage and biologically preventing host bacteria. Due to splitting specificity of the phage, the phage of the specific host strains in the specific environment and a certain state cannot be obtained easily. According to the facts that the vibrio strains and the phage of culture zones and sea areas coexist with the environment ecologically and evolutionally, a long-term culture pool of the host bacteria and the phage is built in a specific area, lytic phage of specific pathogenic bacteria hosts in the area can be continuously enriched and separated, the lytic phage can be placed in the area after augmentation, proliferation of pathogenic bacteria of a cultivation water body can be controlled in a short time, concentration of the pathogenic bacteria of the water body can be restrained in a long term, and the effect of ecological defense is achieved. The method utilizes the phage in the cultivation sea water body to prevent diseases caused by the pathogenic bacteria and has the advantages of being efficient, ecological, safe, safe and lasting.

The invention discloses a compound micro-ecological preparation for degrading nitrite in a culture pond and application. The invention discloses a microorganism preparation for degrading the nitrite in a shrimp pond and inhibiting pathogenic bacteria, in particular relates to the compound micro-ecological preparation for degrading the nitrite, and belongs to the technical field of culture. The micro-ecological preparation, which contains mixed powder and small granules, is prepared by selecting pseudomonas, bacillus amyloliquefaciens and bacillus subtilis which have a denitrification effect, and taking wheat bran, zeolite powder and corn starch as auxiliary materials. According to the compound micro-ecological preparation disclosed by the invention, on one hand, a small molecular carbon source is introduced into a water body, so that the metabolic activity of original denitrifying bacteria and heterotrophic nitrification bacteria of the pond is enhanced; on the other hand, microorganism bacteria play a role on a pond bottom and a water body at the same time through adsorbents with different specific gravities, so that the release of ammonia nitrogen and the nitrite at the pond bottom is reduced; thirdly, the bacillus amyloliquefaciens is utilized, so that the quantity of pathogenic bacteria including vibrio and the like can be inhibited while the water quality is improved, and furthermore, fish diseases are reduced.

The invention provides a microbial preparation for aquaculture water environment ammonia-nitrogen degradation and application thereof, and belongs to the technical field of aquaculture and water quality improvement. The microbial preparation comprises, by mass, 26-50% of rhodopseudomonas palustris PSB20 microbial inoculum and 50-74% of carrier. The rhodopseudomonas palustris PSB20 microbial inoculum is obtained by rhodopseudomonas palustris PSB20 bacterial strain culturing. The preservation number of the rhodopseudomonas palustris PSB20 bacterial strain is CGMCC No. 9632. According to the microbial preparation, ammonia nitrogen and organic matter content in the aquaculture water environment can be obviously reduced, the vibrio total in the aquaculture water environment can be obviously reduced, and efficient aquaculture water quality improvement can be achieved.

The invention discloses a gene chip detecting marine pathogenic vibrios, and a preparation method and a detection method thereof. The gene chip comprises a solid-phase carrier and detection probes fixedly disposed on the solid-phase carrier, and the detection probes comprise vibrio vulnificus gyrB gene probe, vibrio vulnificus virulence gene hemA probe, vibrio splendidus gyrB gene probe, vibrio splendidus virulence gene toxR probe, vibrio harveyi gyrB gene probe, vibrio harveyi virulence gene hemA probe, vibrio parahaemolyticus gyrB gene probe, vibrio parahaemolyticus virulence gene toxS probe, vibrio anguillarum gyrB gene probe and vibrio anguillarum virulence gene hem probe which are respectively shown as SEQ NO 1-10. The gene chip is firstly applied to detection on marine pathogenic vibrios. The gene chip is capable of rapidly sensitively detecting target bacterium infectio, also is good in repeatability and strong in signal, and does not easily cause a nonspecific signal.

The invention discloses a ssDNA nucleic acid aptamer capable of specifically recognizing vibrio alginolyticus, a screening method, a detection method and application thereof. A nucleotide sequence ofthe ssDNA nucleic acid aptamer is 5'-CTTCTATCTACATTTCTTTTTCGTCAATTTTTATTCCCTGGCCCACCCTA-3'(SEQ ID NO:1) or 5'-GACGCTTACTCAGGTGTGACTCG-CTTCTATCTACATTTCTTTTTCGTCAATTTTTATTCCCTGGCCCACCCTA-CGAAGGACGCAGATGAAGTCTC-3'(SEQ ID NO:2). The ssDNA nucleic acid aptamer has specificity and high sensitivity to vibrio alginolyticus and has no immunogenicity. The ssDNA nucleic acid aptamer has the advantages of stable structure, easiness in modification and convenience for synthesis and storage and can be applied in quick and accurate detection and diagnosis of vibrio alginolyticus.

The invention discloses a method for separating denitrification desulfurizing bacteria based on biodiversity information and relates to denitrification desulfurizing bacterium screening methods. The method comprises the steps of collecting sludge, conducting high-throughput sequencing on the sludge sample, analyzing the flora abundance fluctuation and space ecological distribution of the sludge sample, adopting different screening methods according to community composite and structure characteristics, conducting anaerobic culture on a target bacterial genus by means of an interlayer culture medium with different screening methods, selecting a single colony for isolated culture, and obtaining a single bacterial colony after repeated operation. Two kinds of denitrification desulfurizing bacterial strains, namely autotrophic denitrification desulfurizing bacteria which belong to Thiobacillus, and heterotrophic denitrification desulfurizing bacteria which belong to Thauera, Azoarcus, Arcobacter and Ochrobactrum are obtained through screening. Community structure information displayed by the strain screening result is consistent with that displayed by the high-throughput sequencing result, microbial resources are provided for improving biological treatment effectiveness of wastewater. Furthermore, the metabolic characteristics of the strains are identified.

The PCR detection method for the cholerae vibrio, parahaemolyticus vibrio and mimicus vibrio in food, the characteristic is: its detection steps: after the detected sample being secondary enrichment, using boiled method to extract vibrio genomic DNA, and then after PCR reaction, electrophoresis, dyeing, washing, finally using gel imager to observe the result and photograph, and after the spectra analysis, the results can be derived. The invention has the advantage: the simultaneous detection of cholerae vibrio, parahaemolyticus vibrio and mimicus vibrio in food, and can accelerate speed under the conditions of not affecting the detection rate, and can reduce workload and detection cost.

PCT No. PCT / JP97 / 00991 Sec. 371 Date Jan. 6, 1999 Sec. 102(e) Date Jan. 6, 1999 PCT Filed Mar. 25, 1997 PCT Pub. No. WO97 / 35970 PCT Pub. Date Oct. 2, 1997An oligonucleotide is provided which has a nucleotide sequence derived from SEQ ID NO:1, characterized in that it contains at least one site capable of amplifying a nucleotide sequence characteristic of Vibrio parahaemolyticus. The oligonucleotide may have a nucleotide sequence not derived from SEQ ID NO:3, or incapable of amplifying nucleotide sequences originating in Vibrio alginolyticus and Vibrio harveyi, and may be represented by SEQ ID NO:5 or SEQ ID NO:6. A method of detecting Vibrio parahaemolyticus in a specimen is also provided which comprises preparing a primer set comprising two of the above oligonucleotides, selectively amplifying therewith a DNA gyrase subunit B gene sequence contained in the specimen as a target, and determining whether or not there is a gyrB unit specific for Vibrio parahaemolyticus in the specimen. Also provided is a primer which reacts specifically with a gyrB gene of Vibrio parahaemolyticus to thereby differentiate and identify the same among other Vibrios and strains other than the genus Vibrio. The Vibrio parahaemolyticus-specific primer serves to detect 285-bp gyrB gene fragments specific for this Vibrio by the PCR method without the necessity for DNA extraction or like operations from bacterial cells.

The invention belongs to the technical field of research and development of vibrio alginolyticus phage, and in particular, relates to an alkali-resistant vibrio alginolyticus phage and a composition thereof, and a kit. The vibrio alginolyticus phage is vibrio alginolyticus phage VAP21, and has the preservation number of CCTCC NO:M 2018768. The invention also discloses an application of the vibrioalginolyticus phage, or a vibrio alginolyticus phage composition, or a reagent or kit containing the vibrio alginolyticus phage or the vibrio alginolyticus phage composition in killing and / or preventing vibrio microorganisms. The vibrio alginolyticus phage has wide host range, still has high toxicity to host bacteria at low concentration, good proliferation on non-pathogenic bacteria host and large-scale industrial production, and can provide excellent strain resources for an application of phagotherapy.

The invention provides a vibiro splendidus OU02 strain highly yielding alginate lyase, the preservation number of which is CGMCC No.13478. The vibiro splendidus OU02 strain screened by the invention is used for producing and preparing alginate lyase. The alginate lyase produced by the vibiro splendidus OU02 strain screened can degrade alginate to generate alginate oligomers with potential biological activity. The microorganism is short in fermenting period and high in enzymatic activity, and almost activities intracellular activities, so that a sample is favorably collected and stored, thereby laying a foundation for follow-up industrial application.

A process for inhibiting foodborne pathogens and reducing foodborne disease involves contacting a living animal, a dressed carcass, or a cut of meat with an effective amount of a bacillus strain exhibiting antibacterial activity. Strains of antibacterial bacillus that are particularly effective for inhibiting Vibrio were discovered. These include Bacillus licheniformis OBT 712 and Bacillus amyloliquefaciens OBT 618.

The invention discloses a gene chip for detecting a vibrio harveyi colony and a using method of the gene chip. The gene chip is characterized in that nucleotide probes for detecting vibrio alginolyticus, V. campbellii, V. harveyi, V. natriegens, V. parahaemolyticus and V. rotiferianus are immobilized on a chip carrier; the probes of various vibrios use a toxR gene as a target gene; the gene sequences of the various probes are as shown in SEQ ID NO: 11-14; and in a step of PCR amplification on a sample, the gene sequences of a PCR amplification forward primer designed for the toxR gene of various strains of the vibrio harveyi colony and a reverse primer with a digoxin marker are as shown in SEQ ID NO. 15-26. The gene chip disclosed by the invention has the advantage that the gene chip is capable of rapidly and effectively detecting 6 pathogenic vibrios of the pathogenic vibrio harveyi colony in a parallel mode, and the gene chip is high in accuracy and sensitivity and is strong in specificity.