503 results about "Rhodopseudomonas palustris" patented technology

The invention relates to a saline-alkali soil improving agent and its preparation method. The preparation method comprises the following steps: mixing Rhodopseudomonas palustris, Pichia membranefaciens Hansen bacterium, lactobacillus casei, Staestomyces microflavus and seaweed slurry, organically combining a plurality of beneficial microorganisms and seaweed through multi-time fermentation, culturing in a compound mode to obtain a novel microorganism agent-seaweed bio-fungus. adding a proper amount of humic acid, organic matter, active zeolite powder and molasses, fermenting and drying the fermented product. The saline-alkali soil improving agent uses a plurality of microorganisms and employs strong effects of a unique ionic adsorbent, an organic neutralization balancing agent, an ion-exchange agent and a beneficial bacteria conversion starter to achieve the purposes of improving the soil environment, regulating acid-base balance, reducing the salt content, effectively raising the soil fertility and permeability, loosening the soil, eliminating the soil hardening, degrading the harmful substance and promoting the accumulation and conversion of the organic substance.

The invention provides a special bacterial manure for peanut planting, which comprises the following components in parts by weight: 5-25 parts of a compound microbial agent, 30-65 parts of dreg composite powder, 5-20 parts of starch and 10-25 parts of bentonite, wherein the compound microbial agent is composed of bacillus subtilis agent, a rhodopseudomonas palustris agent, a trichoderma harzianum agent and a paecilomyces lilacinus agent; the mass ratio of the bacillus subtilis agent to the rhodopseudomonas palustris agent to the trichoderma harzianum agent to the paecilomyces lilacinus agent is 5:2:1:2, and the total effective viable count of the compound microbial agent is10 billion cfu/g. The bacterial manure is mainly used as a base fertilizer, and can change the microenvironment around the roots of peanuts to prevent various plant diseases and insect pests while promoting nutrient absorption of the peanuts, so that the yield and quality of the peanuts are improved.

The invention discloses a composite microbial agent and a preparation method and application thereof. The composite microbial agent includes a pseudomonas preparation, an acinetobacter preparation, a bacillus amyloliquefaciens preparation, a bacillus subtillis preparation, a bacillus licheniformis preparation, a pediococcus acidilactici preparation, a rhodobacter sphaeroides preparation, an alkali-lake dietzia preparation and a rhodopseudomonas palustris preparation. The preparation method includes the following steps that 1, composite microbial agent components are prepared; 2, the composite microbial agent components are mixed in proportion to obtain the composite microbial agent. The composite microbial agent can simultaneously decompose different organic matter, degrade amino nitrogen and nitro nitrogen in water, reduce phosphorus concentration in the water body and inhibit the growth of algae. The composite microbial agent is used for urban-rural sewage in-situ remediation, has the advantages of being high in efficiency, quick to take effect, free of no secondary pollution, simple in operation and the like, and large-scale application and popularization are facilitated.

The invention applies to provide a microbial deodorant which is a microbial colony composed of lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and rhodopseudomonas palustris, wherein counted by 108CFU (colony-forming unit)/ml as a unit, the total viable count is not less than 10, the lactobacillus plantarum count is not less than 3, the saccharomyces cerevisiae count is not less than 3, the rhodopseudomonas palustris count is not less than 2 and the bacillus subtilis count is not less than 2. The microbial deodorant applied by the invention has stronger zymolysis ability under both aerobic and anaerobic conditions, and the beneficial bacteria mutually play a role in inhibiting the growth of putrefactive bacteria and interrupting the putrefactive process, secrete organic acid while reproducing quickly, and generate antioxidation substances, thus converting odorous substances such as NH3, H2S, indoles and the like into non-odorous substances.

The invention belongs to the technical field of environment protection, and especially relates to a composite microbial preparation for garbage deodorizing and sterilization and a preparation method thereof. The microbial preparation comprises beauveria bassiana, saccharomyces cerevisiae, lactobacillus plantar, rhodopseudomonas palustris, and bacillus thruingiensis. The comprehensive composite inoculant provided by the invention can remove the garbage odor and kill insects such as mosquito, fly, cockroach, and the like; and can be used in locations such as rubbish transfer station, digestion tank, public lavatory, landfill, etc. to remove odors and kill insects. The stinky odors given off by the garbage can be rapidly degraded under the cooperation of five microorganisms, furthermore, the bacterium proliferation is inhibited, the insects in the garbage piles such as mosquito, fly, cockroach, and the like can be killed, and the microecological environment of garbage piles can be improved, so the composite inoculant has a very important economic and environment-protection meaning.

The invention provides a microbial preparation for improving freshwater aquaculture water body. The microbial preparation comprises the following raw materials by weight percentage: 25-30 wt% of bacillus subtilis, 25-30 wt% of bacillus licheniformis and 15-20 wt% of rhodopseudomonas palustris. The invention also provides a method for preparing the microbial preparation, which comprises the following steps of: sequentially inoculating the seed culture liquids of the bacillus subtilis, the bacillus licheniformis and the rhodopseudomonas palustris into a culture medium, fermenting to obtain a bacteria liquid, and mixing the obtained bacteria liquid with a carrier and nutrient substances to obtain the microbial preparation. The microbial preparation provided by the invention can significantly improve the water quality of the aquaculture water body and can also improve the meat quality of bred animals.

This invention relates to a kind of photosynthetic bacterium that has a high assimilation utility to nitrite nitrogen. More specifically, this invention provides a Rhodopseudomonas palustris strain that can assimilate nitrite nitrogen, and its r ejuvenation, preservation, biological detection and application. The photosynthetic bacterium is a kind of biological water purifier and biological fertilizer, and can be used in controlling the content of nitrite nitrogen in water body for aquaculture, improve environmental pollution, and eliminate cinnamic acid and benzoic acid. The photosynthetic bacterium can be used as nitrogen-fixing biological fertilizer and cucumber growth promoter.

The invention discloses a biological organic fertilizer for improving the stress resistance and the output of paddy rice and a preparation method thereof. The biological organic fertilizer comprises the following components: an organic mixture adsorbed with a composite microbe liquid preparation, an organic substance, a Bacillus subtilis inoculum, a Bacillus licheniformis inoculum, a Saccharomyces cerevisiae inoculum, a Rhodopseudomonas palustris inoculum, Lactobacillus plantarum and Lactobacillus acidophilus. The biological organic fertilizer of the invention is rich in beneficial microbe florae and major and trace elements needed by the growth of various crops, so the ecological florae and the trace elements of soil can be supplemented and adjusted, phosphor dissolving and potassium releasing capabilities of soil are enhanced, effective nutrients can be strongly decomposed, converted and synthesized, the fertilizer utilization rate is improved, physical and chemical properties of soil are improved, the granular structure formation is promoted, hardened and morbid soil is restored, the soil fertility is increased, various soil-borne diseases are inhibited, the growth and the fructification of the crops are promoted, and the stress resistance and the output of the crops are substantially improved.

The invention provides a microorganism compound bactericide for controlling cucumber fusarium wilt and a preparation method thereof. The microorganism compound bactericide for controlling cucumber fusarium wilt is characterized by consisting of 10 to 15 parts by weight of rhodopseudanonas palustris, 30 to 40 parts by weight of bacillus subtilis, 15 to 18 parts by weight of streptomyces, 20 to 23 parts by weight of lactobacillu plantarum and 10 to 15 parts by weight of saccharomyces cerevisiae. The preparation method is characterized by comprising the following steps of: screening the rhodopseudanonas palustris, the bacillus subtilis and the streptomyces, and respectively inoculating and culturing in a flask in which nutrient agar culture media are put; mixing obtained strains with the lactobacillu plantarum and the saccharomyces cerevisiae; and inoculating mixed lawn in a liquid culture dish, and fermenting in a fermentation tank to obtain the microorganism compound bactericide. In the invention, soil ecological environment is repaired and improved under the condition of inhibiting the reproduction of fusarium wilt pathogenic bacteria and controlling pathogenetic scale and degree,meanwhile, cucumber growth and yield are improved.

The invention relates to a production method of an agricultural photosynthetic bacteria preparation, adopting the following steps: 1. strain amplification culture: adjusting the pH value of a strain amplification medium from 6.0 to 8.5; placing the medium into a glass bottle and carrying out heat disinfection; inoculating a mixed culture of two photosynthetic bacteria including Rhodopeudomonas palustris and Rhodobacter sphaeroides based on 5-40% of the volume of a strain medium; anaerobically culturing the mixed culture for 3-4 days under the conditions that the temperature is 15-35 DEG C and the illumination intensity is 1000-3000lux, thus obtaining strain culture fluid; 2. fermentation production: placing a fermentation medium into a fermentation cylinder and carrying out heat disinfection; inoculating the strain culture fluid based on the inoculation amount, namely 5-40% of the volume of the fermentation production medium; the strain culture fluid is fermented for 6 days to obtain the agricultural photosynthetic bacteria preparation under the conditions that the temperature is 28-30 DEG C, the stirring rate is 150-300r/m, the cylinder pressure is kept between 0.02MPa and 0.05MPa and the illumination intensity is 2000-3000lux. Compared with the prior art, the preparation has the advantages of stable product quality and diversified functions.

The invention discloses a mixed microbial preparation for aquaculture and a preparation method thereof. The mixed microbial preparation comprises the following bacteria by weight: 30 to 35 parts of Bacillus subtilis, 20 to 30 parts of Bacillus licheniformis, 20 to 25 parts of Bacillus megaterium, 15 to 20 parts of Rhodopseudomonas palustris, 30 to 50 parts of beer yeast, 18 to 20 parts of photosynthetic bacteria, 10 to 13 parts of nitrobacteria, 1 to 5 parts of Streptococcus faecalis, 5 to 10 parts of Bacillus natto and 10 to 15 parts of Bifidobacterium. The mixed microbial preparation is prepared through enlarged cultivation, normal temperature separation, low temperature separation, low temperature drying and the like. The preparation method in the invention has the advantages of easiness, easy practicability and capacity of realizing batch production; the prepared mixed microbial preparation can significantly reduce the content of ammonia nitrogen and nitrite in a water body, inhibit propagation of harmful bacteria, improve water quality and disease resistance of fishes and shrimps, lower down consumption of drugs and antibiotics and effectively improve the quality and the quantity of aquaculture products.

The invention belongs to the technical field of biology and relates to a method for producing a microecological foliar fertilizer by utilizing probiotics and microalgae. The method comprises the following steps of: firstly, culturing to obtain liquid culture prepared by bacillus, lactic acid bacteria and rhodopseudomonas palustris with the cell concentration being 10 billion/ml and saccharomycetes and chlorella with the cell concentration being 2 billion/ml respectively, then preparing liquid culture with four types of probiotics and one type of chlorella, and mixing the liquid cultures according to respective volume ratio to obtain the liquid microecological foliar fertilizer with the total cell concentration being more than 5 billion/ml. The method has the advantages that the used strains and chlorella are all probiotics which can be eaten by human, and are safe and non-toxic; the microecological foliar fertilizer integrates the advantages of promoting the plant growth of the probiotics, the chlorella and metabolites thereof; and the microecological foliar fertilizer is diluted with water by 100 times every 10 days and is sprayed on plant leaves, and the plant yield is increasedby more than 30% in 60 days. The method has an important application value in aspect of improving the yield and the quality of crops such as grain and the like.

The invention relates to the field of microbiology, and specifically relates to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer is composed of a composite microbial fermentation broth and an embedding medium, wherein a volume ratio of the composite microbial fermentation broth to the embedding medium are in a range of 1:5 to 1:4. The compositemicrobial fermentation broth is compounded of a co-culture comprising Candida utilis, lactic streptococci, lactobacillus acidophilus and bacillus subtilis, and a single nutrient solution comprising Bradyrhizobium japonicum, Azospirillum lipoferum, Rhodopseudomonas palustris, Bacillus megaterium, Bacillus mucilaginosus and Azotobacter chrococcum. In the invention, composite microbes processed by embedding can be stored stably for more than 60 days and a count of the composite microbes is always more than 2 billion per gram, thus the composite microbial fertilizer comprising the composite microbes has a long effective time and can improve a planting capacity of soil.

The invention discloses a rhodopseudomonas palustris CCTCC M 2012224, and application of the rhodopseudomonas palustris CCTCC M 2012224 in prevention and control of rice false smut or improvement of catalase activity in the rice body, and further discloses an antagonistic fungicide obtained by activation, seed culture and production culture of the strains. Fermentation supernate obtained through fermentation and centrifugation of the antagonistic fungicide can be used as an antagonistic pesticide; thallus crushing liquid obtained by fermentation culture and crushing of the antagonistic fungicide can also be used as the antagonistic pesticide. The antagonistic fungicide and the antagonistic pesticides obtained both can be used for preventing and controlling the rice false smut and improving the catalase activity in the rice body. The rhodopseudomonas palustris CCTCC M 2012224, the antagonistic fungicide and the antagonistic pesticides have the advantages of low production cost, nontoxicity, environmental friendliness and the like, is convenient to use, and can be used for preventing and controlling the rice false smut and improving the disease resistance of rice.

The invention discloses a probiotics microbial agent for regulating livestock intestinal flora and a preparation method thereof. The probiotics microbial agent is prepared by using candida utilis, streptococcis faecium, bacillus subtilis, bacillus natto, lactobacillus plantarum and rhodopseudomonas palustris as raw materials, performing the three-stage liquid fermentation, then uniformly mixing the enlarged cultivated fermenting bacteria with a feed wheat bran, performing the solid fermentation, drying at low temperature to obtain the probiotics agent for regulating the livestock intestinal flora structure. The agent can be used for regulating the livestock intestinal flora structure, promoting the growth of the probiotics, inhibiting the growth of the harmful bacteria, substituting the antibiotics, improving the feed utilization rate, obviously reducing the odor of the excrement of the livestock and improving the ecological environment of aquiculture and round environment, and is efficient, non-toxic and no residue. The probiotics microbial agent can be widely applied to a livestock feed additive so as to prevent the livestock intestinal disease and respiratory disease.

The invention discloses a microbial preparation for improving an aquatic product culture environment and a preparation method thereof. A strain with optimal nitrite degradation effects is screened and separated from a mud sample in Tongan and Haicang ports of Xiamen city of the Fujian province, another strain with optimal nitrite degradation effects is screened and separated from a mud sample in the intertidal zone of Ningde city of the Fujian province, and the two strains respectively are Rhodopseudomonas palustris PSB20 and Bacillus subtilis ND04. The microbial preparation prepared from the two strains can degrade ammonia nitrogen and nitrous acid in an aquatic product culture environment, has good decomposition effects on residual bait organic matters, has a certain effect of inhibiting pathogenic vibrio bacterium growth in culture water, and has a good production application prospect. The microbial preparation has the characteristic of simple preparation processes.

The invention relates to a microbial agent capable of reducing leaf vegetable plant diseases and insect pests and a preparing method thereof, belonging to the technical field of agricultural production application of microorganisms. The microbial agent capable of reducing leaf vegetable plant diseases and insect pests is characterized in that the microbial agent is prepared from the following rawmaterials: beauveria bassiana culture solution, streptomyces coelicolor culture solution, compound strain culture solution, garlic, molasses, Chinese medicinal herb and trace element inorganic salt solution, wherein the compound strain is composed of Bacillus subtilis culture solution, rhodopseudomonas palustris and saccharomycetes. The preparing method is as follows: respectively culturing beauveria bassiana and streptomyces coelicolor alone, culturing Bacillus subtilis, lactobacillus and saccharomycetes in a compound mode; mixing and fermenting three culturing products, Chinese medicinal herb, garlic and the like; and finally, adding trace elements into the compound fermentation product. The invention can obviously strengthen leaf vegetable organism disease resistance and resilience, improves soil and reduces soil-borne diseases.

The invention relates to the technical field of microbe application, specifically relates to a microbial agent and application thereof to saline-alkali land soil improvement. The microbial agent is prepared by compounding bacillus amyloliquefaciens Bn-21, Bacillus subtilis Bn-3,Bacillus pumilus Bn-19, Rhodopseudomonas palustris Bn-2 and lactobacillus plantarum Bn-23, the compounded multiple microbes has different functions, each microbe carries out different microbe metabolic activity, every two bacterial strains have no antagonism, the function of each microbe is relatively stable, can play a different role in a soil conditioner, increase microbe diversity in soil, enhance activity of the microbe in soil, strengthen the effects of the soil conditioner for improving soil physical and chemical properties and improving the soil fertility.

The invention relates to a preparation method of a selenium enriched rhodopseudomonas palustris preparation, belongs to the technical field of microorganisms, and in particular relates to a liquid anaerobic fermentation method of the selenium enriched rhodopseudomonas palustris preparation. The preparation method comprises the following steps: by using rhodopseudomonas palustris as a fermenting strain, inoculating in an optimized liquid culture medium; cultivating for about 96 hours at 30-35 DEG C; after three-level amplified culture, respectively adding sodium selenite into 25, 50, 75 and 10 mu mg/mL on the second, third, fourth and fifth days; and continuously cultivating to the seventh day, wherein the concentration of selenium in the culture solution is decreased to about 10 mu mg/mL, the conversion ratio of selenium can reach over 90%, the selenium enrichment amount of the strain can reach 75mg selenium/g dry strain, and the organic selenium content is over 97%. The preparation provided by the invention has the advantages that massive fermentation of hodopseudomonas palustris can be completed by a common water white narrow-mouthed bottle and a polyethylene barrel, so that the preparation is simple to operate and low in energy consumption, thus, lowering the production cost and improving the production efficiency. The rhodopseudomonas palustris preparation prepared can be used as a feed additive for animals and further can provide foods rich in organic selenium for human kind.

The invention discloses a novel compound microbial agent used for sludge reduction treatment. The compound microbial agent consists of photosynthetic bacteria, lactic acid bacteria and saccharomycetes, and comprises bacillus subtilis, bifidobacterium animals, bifidobacterium bifidum, bifidobacterium longum, lactobacillus acidophilus, lactobacillus casei, lactobacillus delbrueckii, lactobacillus fermentium, lactobacillus plantarum, lactococcus lactis, rhodopseudomonas palustris, streptococcus thermophilus and saccharomyces cerevisiae which are 13 different microorganisms in total. The compound microbial agent can reduce BOD (biochemical oxygen demand), COD (chemical oxygen demand) and TSS (total suspended solids) to expected values, effectively reduces sludge deposit in a treatment plant, and reduces corrosion on equipment to lower maintenance cost; and besides, bacteria can decompose an organic compound and can generate byproducts beneficial to environment.

The invention discloses a preparation technique of a photosynthetic bacteria reagent, which comprises rhodopseudomonas palustris strain cultivation steps that include activated strain cultivation with an inclined plane, strain cultivation with a shaking table, cultivation with an aerated fermenting jar and the like, and adopts the culture medium comprising a basic culture medium and a growth promoting agent. The basic culture medium comprises following components by mass: 0.9 to 1.1 percent of glucose, 0.04 to 0.06 percent of carbamide, 0.02 to 0.03 percent of dipotassium hydrogen phosphate, 0.02 to 0.03 percent of monopotassium phosphate, 0.02 to 0.03 percent of magnesium sulfate, 0.20 to 0.30 percent of sodium chloride, 0.03 to 0.05 percent of calcium chloride and 98.40 to 98.77 percent of water. The added growth promoting agent is 0.10 to 0.30 percent of fulvic acid by mass referring to the basic culture medium. The photosynthetic bacteria reagent that is obtained by the preparation technique can reach the lysate concentration of 4000 to 6000 million per milliliter, and can be used in multiple fields of aquiculture, wastewater processing, crop production and the like.

The invention belongs to preparation for microorganism-decomposing agents, and in particular discloses a method for preparing a microorganism-decomposing agent used for producing a biological organic fertilizer by livestock and poultry manure fermentation. The method comprises the following steps of: respectively inoculating Saccharomyces cerevisiae AS1.2414 and Saccharomyces cerevisiae AS1.1854 into a culture medium which contains a carbon source, a nitrogen source and nutrient substances for fermentation; inoculating Rhodopseudomonas palustris AS 1.2349 into a culture medium which contains nutrient substances for fermentation; and mixing saccharomyces cerevisiae fermentation liquor, rhodopseudomonas palustris fermentation liquor and rhodopseudomonas palustris fermentation liquor according to a volume ratio of 50:25:25, and taking a mixture of rice bran and bran as a carrier to adsorb the mixed fermentation liquor so as to prepare a solid decomposing agent. The method solves the problems of the quality and the speed of composting influenced by the relatively low composting speed in the composting process of the prior art, and has the advantages of effectively reducing the composting time, improving the compost maturity and the production efficiency, and the like.