Selenium enriched rhodopseudomonas palustris preparation and preparation method thereof
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A technology of swamp rhodopseudomonas and bacterial preparations, applied in biochemical equipment and methods, food preparation, microbial-based methods, etc., to achieve the effects of reduced risk of fermentation failure, stable quality, and low production costs
Inactive Publication Date: 2013-09-11
GUANGZHOU GLAM BIOTECH
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The level of selenium content in cells is the basic indicator of the selenium enrichment ability of bacteria, but the selenium content and the bioma
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Embodiment 1
[0024] Embodiment 1 The technical process for preparing the viable preparation of Rhodopseudomonas palustris comprises the following steps:
[0025] (1) Activation of strains:
[0026] The present invention uses live Rhodopseudomonas palustrias as fermentation strains, purchased from the Guangdong Provincial Microbial Strain Preservation Center, preservation number: GIM1.167, and the live Rhodopseudomonas paluscans preserved at -80°C in Seed medium (peptone 3g, yeast extract 3g, MgSO 4 ·7H 2 O 0.5 g, anhydrous CaCl 2 0.3g, 15g of agar powder, adjusted to pH 7.0, 1000ml of distilled water, high temperature and high pressure sterilization) the slant of the test tube was marked, the light intensity was 3000~5000lx, and cultured at 30°C for 96 hours.
[0027] (2) Preparation of primary seeds:
[0028] Use an inoculation loop to pick a ring of activated live Rhodopseudomonas palustris and inoculate it into 20mL seed medium (3g peptone, 3g yeast extract, MgSO 4 ·7H ...
Embodiment 2
[0036] Embodiment 2 is different from above-mentioned embodiment 1:
[0037](1) The volume of the transparent polyethylene barrel is 15L, the inoculum amount of the expanded culture at all levels is controlled at 25%, and the bacterial cell concentration of the fermentation liquid reaches 3.0×10 9 CFU / mL is about 82 hours of culture, add sodium selenite to 25, 50, 75 and 100ug / mL respectively from the 2nd, 3rd, 4th and 5th day of static culture, and continue to culture until the 7th day, the culture medium The concentration of selenium in the medium drops to about 9.1ug / mL, the conversion rate of selenium reaches 90.9%, the amount of selenium enrichment in the bacteria reaches 75.6mg selenium per gram of dry bacteria, and the content of organic selenium is 97.4%;
[0038] (2) Adsorb the fermented liquid with plant starch: Mix corn flour and wheat flour of equal quality evenly as the adsorbent for the purple-red fermented liquid, dry and dry the fermented liquid mixture abso...
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Abstract
The invention relates to a preparation method of a selenium enriched rhodopseudomonas palustris preparation, belongs to the technical field of microorganisms, and in particular relates to a liquid anaerobic fermentation method of the selenium enriched rhodopseudomonas palustris preparation. The preparation method comprises the following steps: by using rhodopseudomonas palustris as a fermenting strain, inoculating in an optimized liquid culture medium; cultivating for about 96 hours at 30-35 DEG C; after three-level amplified culture, respectively adding sodium selenite into 25, 50, 75 and 10 mu mg/mL on the second, third, fourth and fifth days; and continuously cultivating to the seventh day, wherein the concentration of selenium in the culture solution is decreased to about 10 mu mg/mL, the conversion ratio of selenium can reach over 90%, the selenium enrichment amount of the strain can reach 75mg selenium/g dry strain, and the organic selenium content is over 97%. The preparation provided by the invention has the advantages that massive fermentation of hodopseudomonas palustris can be completed by a common water white narrow-mouthed bottle and a polyethylene barrel, so that the preparation is simple to operate and low in energy consumption, thus, lowering the production cost and improving the production efficiency. The rhodopseudomonas palustris preparation prepared can be used as a feed additive for animals and further can provide foods rich in organic selenium for human kind.
Description
[0001] technical field [0002] The invention relates to the field of microbial fermentation engineering, in particular to a method for preparing selenium-enriched Rhodopseudomonas palustris through anaerobic fermentation of a liquid medium added with sodium selenite. [0003] Background technique [0004] Rhodopseudomonas palustris ( Rhodopseudomonas palustris ) In biological classification, it belongs to Eubacteria, Rhodospirillales, Rhodospirillaceae, Rhodopseudomonas, and Rhodopseudomonas palustris. Carry out photosynthesis without oxygen release, can use a variety of organic substances, and can carry out heterooxygen growth with various organic substances and light energy. Its bacteria are rich in protein and B vitamins and can promote the growth and development of animals. The physiologically active substances can also play the role of probiotics, and can be used in microbial feed additives, livestock and poultry aquaculture. [0005] People are paying more and more...
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