138 results about "Animal feed additive" patented technology

The invention discloses a composite antibacterial preparation containing antibacterial peptide and traditional Chinese medicines as well as a preparation method and application of the composite antibacterial preparation, and belongs to the technical field of biological medicines. The antibacterial peptide provided by the invention is a transmembrane protein derived from bacteriophage, and the core function of the antibacterial peptide is that bacterial cell lysis is promoted by destroying a connection structure of a peptidoglycan layer and lipoprotein of a bacterial cell wall, so that an antibacterial effect is achieved. The amino acid sequence of the antibacterial peptide is shown as SEQ ID NO: 3, the antibacterial peptide is combined with traditional Chinese medicines to form a composite antibacterial preparation, and experiments prove that the composite antibacterial preparation has a relatively good antibacterial effect on gram-positive bacteria and gram-negative bacteria and can be applied to the medical field, such as surgical instrument disinfection and drug-resistant bacterial infection treatment; the field of daily chemicals, such as antibacterial hand sanitizers and wound dressings; in the field of agriculture, for example, animal feed additives for preventing and treating intestinal infection have a good market application prospect.

The invention belongs to the field of animal feed additives, and particularly relates to a microcapsule preparation containing origanum vulgare essential oil as well as a preparation method and application of the microcapsule preparation. The preparation method of the microcapsule preparation containing origanum vulgare essential oil comprises the following steps: S1, dissolving modified beta-cyclodextrin and modified starch in water to obtain a wall material solution; s2, uniformly mixing oregano essential oil to prepare a core material solution, and dissolving an emulsifier in the core material solution to form an oil phase; s3, mixing the wall material solution with an oil phase, and homogenizing to obtain a solution A; and S4, adding a stabilizer into the solution A, uniformly mixing, and spray-drying to obtain the microcapsule preparation containing the origanum vulgare essential oil. The oregano essential oil entrapped microcapsule preparation is prepared by adopting the modified beta-cyclodextrin as a wall material and the modified montmorillonite as a stabilizer, so that the stability and the entrapment efficiency of the oregano essential oil are remarkably improved, the intestinal targeted release of the oregano essential oil is also realized, and the growth and development of animals are promoted.

The invention provides a feed additive capable of improving immunity of pet cats as well as a preparation method and application of the feed additive, and belongs to the technical field of animal feed additives. The feed additive comprises probiotics and a traditional Chinese medicine extract, the probiotics comprise bifidobacterium animalis, combined lactobacillus salivarius and saccharomyces cerevisiae boulardii, and the traditional Chinese medicine extract comprises astragalus polysaccharide, curcumin and resveratrol. The probiotics directly enhance intestinal immunity through colonization resistance and metabolites, the traditional Chinese medicine components form internal and external dual defense through system immune activation, immunity-flora dual regulation is achieved, the probiotics and the traditional Chinese medicine extract form a symbiotic system of increasing the drug effect through bacteria and protecting the bacteria through drugs, and 1 + 1gt is achieved; and 2, the immunity improving effect is achieved.

The invention belongs to the field of microorganisms, and particularly relates to lactococcus lactis Jlus 880314 and application thereof. The strain is classified and named as Lactococcus lactis, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation number of the strain is CGMCC No. 33961. Time-of-flight mass spectrometry analysis shows that nisin exists in a metabolite of the strain, and the strain can be used for producing nisin. The compound shows an excellent and wide antibacterial function in antibacterial experiments, can be used for preparing antibacterial drugs, particularly has a remarkable inhibiting effect on growth of gram-negative bacteria and fungi, can be used for preparing drugs for relieving or treating enteritis, or preparing drugs, nourishment or health care products for regulating gastrointestinal tract micro-ecological balance of people or animals, and can be used for preparing medicines for relieving or treating enteritis. The compound can also be used for preparing antibacterial food additives and / or animal feed additives. A hemolysis experiment result shows that hemolysis is avoided, toxic and side effects are small, and wide development prospects and application values are achieved.

The invention discloses a compound rumen bypass guanidinoacetic acid additive as well as a preparation method and application thereof, and belongs to the technical field of animal feed additives. The compound rumen bypass guanidinoacetic acid additive provided by the invention is prepared from the following components in parts by mass: 40 to 60 parts of guanidinoacetic acid, 0.1 to 0.5 part of selenium yeast, 5 to 10 parts of nicotinamide, 5 to 12 parts of DL-methionine, 0.01 to 0.05 part of vitamin B12, 1 to 3 parts of compound probiotics, 3 to 5 parts of hydroxypropyl methyl cellulose, 1 to 2 parts of ethyl cellulose, 15 to 25 parts of hydrogenated palm oil and 3 to 5 parts of glyceryl monostearate. The compound probiotics comprise bacillus subtilis and saccharomyces cerevisiae. The compound additive provided by the invention can significantly improve the daily gain of ruminants and reduce the feed-gain ratio, and meanwhile, due to the improvement of the energy utilization efficiency and the enhancement of the antioxidant ability, the immunity of animals is improved, the morbidity is reduced, and the meat quality is improved.

Bacillus strains useful for preventing and / or controlling Clostridium perfringens infections and necrotic enteritis in monogastric animals, such as swine and poultry, and for improving the overall health, feed conversion rates and body weight gain of such animals, as well as compositions comprising the Bacillus strains and various methods of using the Bacillus strains in animal feed and animal feed additives.

Novel polypeptides having lysozyme activity, polynucleotides encoding polypeptides having lysozyme activity, and nucleic acid constructs, vectors, and host cells comprising polynucleotides that encode polypeptides having lysozyme activity, as well as methods of producing and using such polypeptides, polynucleotides, constructs, vectors, and host cells, including, but not limited to, animal feed and animal feed additives comprising polypeptides having lysozyme activity.

The present invention relates to the use of alpha lipoic acid as feed additive for aquatic animals including fish and shrimp, especially for cold water fish as for example salmon, bream, bass and for warm water fish as for example carp, tilapia, catfish. More particular, this invention relates to the use of alpha lipoic acid for the improvement of the feed conversion ratio and / or daily weight gain in fish, for reducing mortality by regulating the micro flora of the gut and / or by protecting the animal against infections caused by pathogenic viruses.

Novel polypeptides having lysozyme activity, polynucleotides encoding polypeptides having lysozyme activity, and nucleic acid constructs, vectors, and host cells comprising polynucleotides that encode polypeptides having lysozyme activity, as well as methods of producing and using such polypeptides, polynucleotides, constructs, vectors, and host cells, including, but not limited to, animal feed and animal feed additives comprising polypeptides having lysozyme activity.

The invention relates to the technical field of animal feed preparation, in particular to an animal feed additive containing microbial muraplase and a preparation method thereof.The method comprises the steps that additive raw materials of a target animal and a feeding environment are selected, smashed and screened, and pretreated raw materials are obtained; grading and mixing the raw materials to obtain mixed raw materials, detecting distribution and particle uniformity, and determining grading and mixing parameters and crushing rotating speed based on a comparison result; and determining an additive forming judgment value based on the actual uniformity, starting granulation in combination with a threshold value to obtain actual granulation particles, detecting the granulation hardness of the particles, and secondarily determining the distribution uniformity based on a granulation hardness comparison result. The grading mixing parameters are adjusted by detecting the distribution uniformity and the particle uniformity of the mixed raw materials, the additive forming judgment value is determined based on the distribution uniformity and the particle uniformity so as to control granulation, the distribution uniformity is secondarily verified through the granulation hardness comparison result, and the stability of the active ingredients of the additive and the feed digestibility are improved.

The invention discloses a phytase mutant with improved enzymatic activity and thermal stability as well as a preparation method and application thereof, and relates to the technical field of gene engineering and enzyme engineering. The amino acid sequence of the phytase mutant (named as Y111C / Q113L) is as shown in SEQ ID NO. 1. According to the invention, site-directed mutagenesis is carried out on amino acid residues which may influence the enzymatic activity and thermal stability of phytase YrAPPA in phytase YrAPPA, and mutants Y111C and Q113L with significantly improved enzymatic activity and thermal stability are constructed and screened. On the basis, the two-site mutant Y111C / Q113L with the phytase activity and the thermal stability further improved is further constructed and obtained. The enzyme activity and thermal stability of the phytase mutant provided by the invention are obviously improved, and the phytase mutant has a good application prospect in the field of animal feed additives.

A process and a chemical production unit for producing crystalline ammonium chloride in the presence of a crystallization additive are provided, wherein the process comprises the steps a) reacting NH3 and HCl by feeding NH3 and HCl to an aqueous solution of ammonium chloride; b) crystallizing ammonium chloride from the aqueous ammonium solution obtained in step a), and c) separating the crystalline ammonium chloride, wherein energy required in step b) is generated in step a). The crystalline ammonium chloride, obtainable by said process, is suitable as a flavoring agent, as an animal feed additive, as an additive for a cosmetic composition or as an additive for a pharmaceutical composition.

Protein-rich nutrient supplements and animal feed supplements derived from an anaerobic bacterial process are generated through a myriad of cell rupturing and protein fractionation / purification processes. Bacterial fermentation systems and methods of obtaining one or more protein-containing portions from a fermentation process using carbon monoxide-containing gaseous substrates are provided. The invention further provides compositions of protein-rich nutrient supplements with useful applications for intake by a variety of different animals and humans.

The invention discloses an animal feed additive composition, an animal feed and application of the animal feed. The animal feed additive comprises marine animal-derived hydroxyapatite, takifugu fish skin glue, composite algae powder and euphausia superba powder. Marine animal source hydroxyapatite is derived from takifugu calthrop bone meal. The animal feed additive composition comprises the following components in percentage by mass: 40%-50% of takifugu calthrop bone meal; 10%-15% of takifugu fish skin glue; 30%-40% of composite algae powder; and 10%-15% of euphausia superba powder. The invention also discloses an animal feed, which comprises a basal feed and the animal feed additive composition accounting for 3.0-6.0% of the mass of the basal feed. According to the application, the takifugu calthrops are converted into the animal feed additive composition with high additional value from fish skin processing waste for the first time, so that single-component chemical additives such as stone powder and calcium hydrophosphate which are widely used at present can be replaced, and resource utilization and high-value utilization of marine fishery waste are realized.

The present invention relates to the use of an animal feed additive composition for controlling the growth of undesirable microorganisms, said composition comprising glycerides, vegetable oils, tannins and / or saponins, the glycerol ester is selected from the group consisting of glycerol monopropionate, glycerol dipropionate and glycerol tripropionate, glycerol monobutyrate, glycerol dibutyrate and glycerol tributyrate, glycerol monovalerate, glycerol divalerate and glycerol trivalerate, and mixtures thereof. The invention also relates to the use of the animal feed additive composition for promoting intestinal growth, alleviating or alleviating effects from necrotizing enteritis, modulating intestinal flora and / or as an additive to drinking water.

This invention discloses the application of a porcine-derived Bacillus in antioxidant activity, wherein the porcine-derived Bacillus is used to prepare antioxidant products. The porcine-derived Bacillus is Bacillus subtilis YB-X-721, classified as Bacillus subtilis, and was deposited at the China General Microbiological Culture Collection Center on November 10, 2023, with accession number CGMCC No. 28942. The fermentation supernatant, bacterial suspension, and cell-free extract of the porcine-derived Bacillus subtilis YB-X-721 of this invention all possess certain DPPH free radical, superoxide anion free radical, and hydroxyl free radical scavenging abilities, as well as a certain reducing ability, and can be used to prepare antioxidant products. Furthermore, when the porcine-derived Bacillus subtilis YB-X-721 of this invention is added to piglet feed as an animal feed additive, it can improve the growth performance of weaned piglets, significantly increasing average daily weight gain, and significantly reducing average daily feed intake, feed conversion ratio, and diarrhea rate.

The invention provides application of a TRPM8 inhibitor in improving the production performance of animals under cold conditions and a method. Specifically, the invention provides application of a TRPM8 inhibitor in preparation of a product for reducing adaptive heat production of animals under cold conditions and a method for reducing adaptive heat production of animals under cold conditions, and the method comprises the following steps: adding the TRPM8 inhibitor into animal feed to obtain mixed feed; and feeding the animal with the mixed feed. The TRPM8 inhibitor is used as an animal feed additive, so that adaptive heat production of animals is reduced, and production traits and economic values of the animals are improved.

This invention relates to an endogenous antimicrobial peptide from bovine milk and its applications. The antimicrobial polypeptide compound VAVALARPKHPIK is derived from endogenously enzymatically hydrolyzed bovine casein, with the amino acid sequence Val-Ala-Val-Ala-Leu-Ala-Arg-Pro-Lys-His-Pro-Ile-Lys. It exhibits broad inhibitory activity against both Gram-positive and Gram-negative bacteria, including *Porphyromonas gingivalis*, *Escherichia coli*, and *Staphylococcus aureus*. This antimicrobial peptide shows promising applications as a health supplement and / or medical device or pharmaceutical for the prevention and / or reduction of infectious diseases, as a food antimicrobial agent, in novel functional foods, pet food and animal feed additives, and in daily chemical products.

The invention discloses a fermentation preparation method of gastrointestinal tract health care traditional Chinese medicine for animals in the technical field of animal feed additives. The method comprises the following steps: crushing traditional Chinese medicines such as astragalus membranaceus, poria cocos, bighead atractylodes rhizome, hawthorn, pericarpium citri reticulatae and liquorice, mixing the crushed traditional Chinese medicines with wheat bran, adding composite probiotics and unique dynamic covalent bonding plant polysaccharide polyphenol three-dimensional network carrier material fine powder, humidifying, fermenting at constant temperature, and finally drying and crushing to obtain the product. The key point is that the carrier material is formed by assembling oxidized sodium alginate, hydrazide carboxymethylated yeast glucan and tea polyphenol tannic acid composite nano-colloid through the synergistic effect of dynamic covalent bonds and hydrogen bonds. The material not only can remarkably improve the survival rate and metabolic activity of probiotics in a fermentation system and promote conversion of active ingredients of traditional Chinese medicines, but also has intelligent responsiveness and can realize targeted slow release in animal intestines, so that the gastrointestinal tract health-care function of a product is comprehensively enhanced, and the growth performance of animals is improved.

The invention provides a feed additive for preventing sheep brucellosis and a preparation method thereof, and belongs to the technical field of animal feed additives. The feed additive for preventing sheep brucellosis is prepared from the following components in parts by weight: 25-35 parts of radix scutellariae, 18-24 parts of herba houttuyniae, 15-20 parts of herba andrographitis, 12-16 parts of herba portulacae, 10-15 parts of radix angelicae sinensis, 10-15 parts of colla corii asini, 5-10 parts of rhizoma atractylodis macrocephalae, 4-8 parts of coix seeds and 3-5 parts of radix glycyrrhizae. The components of the feed additive are used together, so that the feed additive can inhibit the growth and reproduction of brucella, regulate the gastrointestinal health of animals, improve the immunity and enhance the disease resistance of the animals, thereby effectively preventing and controlling sheep brucellosis. The feed additive disclosed by the invention is good in prevention and treatment effect, small in toxic and side effects and not easy to generate drug resistance.

Applications of a diphenylpropenone compound of general formula (I), or a stereoisomer thereof, or a geometric isomer thereof, or a tautomer thereof, or a solvate thereof, or an acceptable animal feed salt, in the preparation of an animal feed additive or feed. Confirmed by animal breeding trials, the diphenylpropenone compound, or a stereoisomer thereof, or a geometric isomer thereof, or a tautomer thereof, or a solvate thereof, or an acceptable animal feed salt, can be used as the animal feed additive or animal feed. The weight of the animals can be effectively increased, survival rates can be enhanced, and the compound has a good effect on improving animal production performance.

Application of total monoterpene glycosides of Paeonia veitchii Lynch and analysis method for active ingredients in total monoterpene glycosides of Paeonia veitchii Lynch. For the first time, the UPLC-MS / MS technology is adopted in the present invention to establish the fingerprint of total monoterpene glycosides of Paeonia veitchii Lynch from 11 batches of different producing areas, laying a foundation for revealing the effective ingredient group of total monoterpene glycosides of Paeonia veitchii Lynch. Five compounds with muscle-building effects mainly composed of total monoterpene glycosides of Paeonia veitchii Lynch are determined in the present invention, including 4 monoterpene glycoside compounds, and one belongs to the isomer of benzoylpaeoniflorin. Application of total monoterpene glycosides of Paeonia veitchii Lynch in preparing animal feed or medicine for increasing muscle. The present invention establishes a fingerprint-pharmacodynamic correlation model of total monoterpene glycosides of Paeonia veitchii Lynch. While establishing the quality evaluation standard for muscle-building effects based on the fingerprint of the effective part of total monoterpene glycosides of Paeonia veitchii Lynch, it also provides a new idea for screening active ingredients of other pharmacological effects of Paeonia veitchii Lynch. The present invention lays a theoretical foundation for the development of muscle-building foods for fitness people and provides new resources for the development of animal feed additives in animal husbandry.

The present invention relates to a composition for enhancing immunity, containing Cheongwi-san as an active ingredient. The Cheongwi-san of the present invention is a mixed herbal medicine extract consisting of Cimicifuga heracleifolia, Moutan Radicis Cortex, Angelica gigas, Coptis Rhizome, and fresh root of Rehmannia glutinosa. The Cheongwi-san of the present invention has excellent effects of increasing the activity of NK cells, increasing the production amounts of IFN-γ and TNF-α, and reducing the survival rate of cancer cells, and has the effect of inhibiting influenza virus infection, and thus can be effectively used as a health functional food for enhancing immunity, a feed additive for animals for enhancing immunity, or a pharmaceutical product for preventing and treating diseases caused by decreased immunity.

The present invention provides an animal feed additive with anti-inflammatory and antioxidant functions, namely, a traditional Chinese medicine-type feed additive with anti-biotic efficacy. The preparation method comprises the following steps: mixing a dried olive pomace powder sample with a subcritical extractant, performing subcritical extraction, and obtaining an extraction system; removing the subcritical extractant from the extraction system to obtain a pomace powder system from which pomace oil has been removed; mixing the pomace powder system with ethanol, performing leaching, and subjecting the obtained extract to vacuum distillation to obtain an extract; mixing the extract system with petroleum ether, allowing the petroleum ether phase to stand for stratification, and filtering the obtained petroleum ether phase to obtain an extract. The maslinic acid and oleanolic acid extracted by the present invention have anti-inflammatory and antioxidant effects, and animal experiments have confirmed that they can enhance intestinal health in a short period of time. The addition of a polypeptide improves the side effects of the maslinic acid and oleanolic acid caused by their long-term use. The present invention can be promoted and used as a new animal feed additive.

The purpose of the present invention is to provide a feed additive composition for ruminants containing 30% by weight or more of a biologically active, dispersed substance that is highly protective in the intestinal lumen and has excellent solubility in the gastrointestinal tract. The present invention relates to a feed additive composition for ruminants characterized by containing (A) at least one hydrogenated vegetable oil or hydrogenated animal oil with a melting point above 50°C and below 90°C, (B) between 0.05% and 6% by weight of lecithin, (C) between 30% and 65% by weight of a biologically active substance, (D) between 0.01% and 0.8% by weight of a natural vegetable oil, and between 0.1% and 6% by weight of water.

The utility model relates to the technical field of animal feed additive production, in particular to an anti-blocking blanking device for animal feed additive processing production, which is characterized in that caked precipitates are crushed through a crushing mechanism and a flushing mechanism, and then the animal feed additive is discharged through a discharging mechanism. Blockage in animal feed additive production equipment and pipelines is prevented, so that the practicability of the equipment is improved; comprising a discharging mechanism; the device further comprises a smashing mechanism and a flushing mechanism, and the smashing mechanism and the flushing mechanism are both installed on the discharging mechanism. The discharging mechanism is used for conveying animal feed additives, and the crushing mechanism and the flushing mechanism are used for crushing caked precipitates.

The present invention relates to a novel B. velezensis strain that improves the health and performance of animals. Another aspect of the present invention relates to using the novel strain to inhibit or reduce pathogen growth in animal feed, for instance as an animal feed additive. Another aspect of the present invention relates to a direct-fed microbial composition, wherein administration of the composition results in an improved performance parameter. Another aspect of the present invention relates to a novel strain that can be incorporated into food or foodstuff.

The application discloses a kind of hydroxyl methionine chelate and its preparation method and application, chelate can be made animal feed additive and nutrient agent, for binuclear chelate system, chelate structure A and chelate structure B include central iron ion and hydroxyl methionine ligand;The carbonyl positive coordination connection of central iron ion of chelate structure A is connected with the hydroxyl methionine ligand of chelate structure B;Preparation method includes: hydroxyl methionine is dissolved, and ligand neutralizing liquid is prepared;S2, ligand neutralizing liquid is mixed with ferrous chloride tetrahydrate solution uniformly, and reaction is complete.The hydroxyl methionine iron chelate of the application is strong in stability, can keep the biological activity of iron in complex chyme environment, can avoid absorption antagonism with manganese, copper and other trace elements, optimizes overall mineral utilization efficiency, significantly improves the absorption rate of animal, preparation condition is mild, cost is controllable, compatible with existing feed production line, and industrialization prospect is good.

The present invention relates to a novel B. velezensis strain that improves the health and performance of animals. Another aspect of the present invention relates to using the novel strain to inhibit or reduce pathogen growth in animal feed, for instance as an animal feed additive. Another aspect of the present invention relates to a direct-fed microbial composition, wherein administration of the composition results in an improved performance parameter. Another aspect of the present invention relates to a novel strain that can be incorporated into food or foodstuff.

This invention discloses two strains of lactic acid bacteria and their applications. The two strains are *Enterococcus faecium* YY1-1 and *Enterococcus faecalis* YY9-1, deposited at the China Center for Type Culture Collection (CCTCC), with accession numbers CCTCC NO: M 2023685 and CCTCC NO: M 2023686, respectively. The two lactic acid bacteria isolated by this invention exhibit superior probiotic properties and strong safety. When mixed with feed, they can improve the growth performance, intestinal digestive enzyme activity, serum non-specific immune activity, and resistance to *Edwardsiella pneumoniae* infection in yellow catfish. Therefore, these two lactic acid bacteria have the potential to be used as feed additives for aquatic animals and also show broad prospects in the prevention and control of aquatic animal diseases.