7 results about "Phytase activity" patented technology

The present application belongs to the technical field of feed processing, and provides a puffed fermented phosphorus-reduced high-digestive-utilization rice by-product feed, which adopts a multi-layer structure design of CO2 guided puffed foaming extrusion to form a porous base material, sodium alginate and chitosan are alternately deposited to construct an interface modification layer, and compound enzymes and probiotics are solidly fermented, three-dimensional pore structure with a median pore size of 20-60 mu m and a connected pore ratio of greater than or equal to 60%, phytase activity of 5000-10000 FTU / kg, total viable bacterial count of greater than or equal to 1x10 8 CFU / g, and an acid value increment of less than or equal to 2 mgKOH / g after 90 days of storage, solves the technical problems of low utilization of rice by-product phytic acid phosphorus, low survival rate and easy inactivation of compound enzymes and probiotics, and poor storage stability of high-oil rice bran, and has wide application value in the feed industry.

This invention relates to the fields of microbiology and biotechnology, and discloses Bacillus coagulans PPX-13 and its applications. Strain PPX-13 is a thermotolerant, multifunctional phosphate-solubilizing biocontrol bacterium that can reduce rhizosphere soil acidification in tea trees, improve fertility, enhance the microbial ecological environment, improve the functional microbial community and structure, inhibit mold communities and structure, and increase the yield of spring tea. It has been identified as Bacillus coagulans. This bacterium exhibits mesophilic and thermotolerant phosphate-solubilizing activity; phytase activity; multiple enzyme activities; and mesophilic antagonistic activity against isolates of anthracnose fungal pathogens. This strain holds promise for development into a novel agricultural microbial agent, which is of great significance for mitigating soil acidification, reducing fertilizer use, activating soil nutrients, improving soil quality, and increasing plant (especially tea) yield and quality.

The present invention discloses novel phytases that have improved phytase activity, compositions comprising them, recombinant host cells suitable for their production, and their use in feed applications.

The present invention relates to a synthetic phytase which comprises (a) the amino acid sequence as set forth in SEQ ID NO. 1, (b) a variant of (a) having at least 70% sequence identity to SEQ ID NO. 1, or (c) a fragment, fraction or shuffled variant of (a) or (b) maintaining phytase activity, with the proviso that the phytase comprises one or more amino acid substitutions at one or more positions relative to SEQ ID NO. 1 selected from the group consisting of V13, T30, N37, K45, L46, T81, N83, A89, A113, Q121, Q122, A123, H128, K131, K139, A142, H143, Q162, S164, A166, S170, Q182, N184, G186, K187, L188, A194, M195, A200, I201, N202, D204, N206, K207, A209, S218, T219, L225, H228, K234, N239, E243, S248, Q256, F257, M260, S261, N270, P288, P292, A311, A314, L319, S320, W321, K344, K347, M355, L359, Q365, P367, E372, T388, Q404, and N405.

The invention relates to solid animal feed compositions produced by a steam-pelleting process, where the compositions comprise a protein component and an enzyme, e.g. a polypeptide having phytase activity, wherein the enzyme, e.g. the polypeptide having phytase activity, is formulated in granules comprising a core, the enzyme, e.g. the polypeptide having 5 phytase activity, and an outer salt coating, and wherein the animal feed composition further comprises a short-chain aldehyde such as formaldehyde or an organic acid or salt thereof.

The application discloses a phytase-pullulanase fusion protein Y-L4-T and application thereof, and belongs to the technical field of enzyme engineering. The amino acid sequence of the phytase-pullulanase fusion protein Y-L4-T is shown as SEQ ID NO. 46. The application constructs a phytase-pullulanase fusion protein Y-L4-T, the phytase activity of the fusion protein is basically consistent with that of phytase YiAPPA, and the amylase activity is significantly higher than that of pullulanase TK-PUL. After the wheat bread is treated by the fusion protein, the specific volume of the wheat bread is obviously increased, the hardness is obviously reduced, and the elasticity is obviously improved; and the increase of the hardness and the reduction of the elasticity of the wheat bread during storage can be effectively alleviated. The fusion protein Y-L4-T can effectively improve the bread quality and prolong the shelf life of the bread, and has a good application prospect in the baking field.

The application discloses a processing method of high-nutrition germinated brown rice with acid-induced phytase activity, and comprises the following steps: immersing disinfected brown rice into a citric acid-vitamin C solution with a concentration of 0.5-0.7 %, and then performing low-frequency ultrasonic soaking at 25-30 DEG C for 30 min, and then cleaning the brown rice with purified water to obtain pre-soaked brown rice; immersing the pre-soaked brown rice into purified water at 35-38 DEG C, and then soaking for 6-8 h until the water absorption rate is greater than or equal to 30 %, wherein the water temperature is adjusted to 40 DEG C plus or minus 1 DEG C in the last 2 h of the soaking, and then maintained for 30 min to obtain soaked brown rice; and drying and sterilizing the soaked brown rice after germination for 24-36 h to obtain high-nutrition germinated brown rice. The application utilizes citric acid to induce and activate phytase activity in the brown rice, thereby improving the phytate degradation rate of the brown rice and the absorption rate of trace elements.