14 results about "Phytase activity" patented technology

The invention discloses a phytase mutant with improved enzymatic activity and thermal stability as well as a preparation method and application thereof, and relates to the technical field of gene engineering and enzyme engineering. The amino acid sequence of the phytase mutant (named as Y111C / Q113L) is as shown in SEQ ID NO. 1. According to the invention, site-directed mutagenesis is carried out on amino acid residues which may influence the enzymatic activity and thermal stability of phytase YrAPPA in phytase YrAPPA, and mutants Y111C and Q113L with significantly improved enzymatic activity and thermal stability are constructed and screened. On the basis, the two-site mutant Y111C / Q113L with the phytase activity and the thermal stability further improved is further constructed and obtained. The enzyme activity and thermal stability of the phytase mutant provided by the invention are obviously improved, and the phytase mutant has a good application prospect in the field of animal feed additives.

The present application belongs to the technical field of feed processing, and provides a puffed fermented phosphorus-reduced high-digestive-utilization rice by-product feed, which adopts a multi-layer structure design of CO2 guided puffed foaming extrusion to form a porous base material, sodium alginate and chitosan are alternately deposited to construct an interface modification layer, and compound enzymes and probiotics are solidly fermented, three-dimensional pore structure with a median pore size of 20-60 mu m and a connected pore ratio of greater than or equal to 60%, phytase activity of 5000-10000 FTU / kg, total viable bacterial count of greater than or equal to 1x10 8 CFU / g, and an acid value increment of less than or equal to 2 mgKOH / g after 90 days of storage, solves the technical problems of low utilization of rice by-product phytic acid phosphorus, low survival rate and easy inactivation of compound enzymes and probiotics, and poor storage stability of high-oil rice bran, and has wide application value in the feed industry.

This invention relates to the fields of microbiology and biotechnology, and discloses Bacillus coagulans PPX-13 and its applications. Strain PPX-13 is a thermotolerant, multifunctional phosphate-solubilizing biocontrol bacterium that can reduce rhizosphere soil acidification in tea trees, improve fertility, enhance the microbial ecological environment, improve the functional microbial community and structure, inhibit mold communities and structure, and increase the yield of spring tea. It has been identified as Bacillus coagulans. This bacterium exhibits mesophilic and thermotolerant phosphate-solubilizing activity; phytase activity; multiple enzyme activities; and mesophilic antagonistic activity against isolates of anthracnose fungal pathogens. This strain holds promise for development into a novel agricultural microbial agent, which is of great significance for mitigating soil acidification, reducing fertilizer use, activating soil nutrients, improving soil quality, and increasing plant (especially tea) yield and quality.

The invention belongs to the technical field of feed processing, and provides a puffing fermentation phosphorus-reducing rice byproduct feed with high digestion utilization rate, which adopts a multi-layer structure design that CO2-guided puffing foaming extrusion is adopted to form a porous base material, sodium alginate and chitosan are alternately deposited to construct an interface modification layer, and compound enzyme and probiotics are immobilized for fermentation. The technical performance that the three-dimensional pore channel structure with the pore diameter median value being 20-60 micrometers and the communicating pore proportion being larger than or equal to 60% is achieved, the phytase activity is 5000-10000 FTU / kg, the total viable count is larger than or equal to 1 * 10 < 8 > CFU / g, and the acid value increment is smaller than or equal to 2 mgKOH / g after storage for 90 days is achieved, the technical problems that the rice byproduct phytate phosphorus utilization degree is low, the survival rate of compound enzyme probiotics is low, the compound enzyme probiotics are prone to inactivation, and the rice bran is high in oil, prone to oxidation and poor in storage stability are solved. The method has a wide feed industrial application value.

The present invention discloses novel phytases that have improved phytase activity, compositions comprising them, recombinant host cells suitable for their production, and their use in feed applications.

The present invention relates to a synthetic phytase which comprises (a) the amino acid sequence as set forth in SEQ ID NO. 1, (b) a variant of (a) having at least 70% sequence identity to SEQ ID NO. 1, or (c) a fragment, fraction or shuffled variant of (a) or (b) maintaining phytase activity, with the proviso that the phytase comprises one or more amino acid substitutions at one or more positions relative to SEQ ID NO. 1 selected from the group consisting of V13, T30, N37, K45, L46, T81, N83, A89, A113, Q121, Q122, A123, H128, K131, K139, A142, H143, Q162, S164, A166, S170, Q182, N184, G186, K187, L188, A194, M195, A200, I201, N202, D204, N206, K207, A209, S218, T219, L225, H228, K234, N239, E243, S248, Q256, F257, M260, S261, N270, P288, P292, A311, A314, L319, S320, W321, K344, K347, M355, L359, Q365, P367, E372, T388, Q404, and N405.

The invention relates to solid animal feed compositions produced by a steam-pelleting process, where the compositions comprise a protein component and an enzyme, e.g. a polypeptide having phytase activity, wherein the enzyme, e.g. the polypeptide having phytase activity, is formulated in granules comprising a core, the enzyme, e.g. the polypeptide having 5 phytase activity, and an outer salt coating, and wherein the animal feed composition further comprises a short-chain aldehyde such as formaldehyde or an organic acid or salt thereof.

A process for producing a protein isolate from an oilseed meal, and the isolate thus obtained, said isolate comprising proteins and an amount of 4 wt % or less of phytic acid, said amount of phytic acid being by weight of proteins in said isolate. The process may comprise the following steps: a) providing an oilseed meal; b) mixing the oilseed meal with a first aqueous solvent to form a slurry at a pH ranging from 6 to 7.8, said slurry having a solid phase; c) separating said solid phase from said slurry, d) mixing said separated solid phase with a second aqueous solvent at a pH ranging from 1 to 3.5, preferably from 2 to 3, to form a mixture said mixture having a liquid phase; e) separating said liquid phase from said mixture formed in step d); f) f1) mixing the separated liquid phase to a phytase at a temperature and a pH suitable for phytase activity to obtain a mixture having a liquid phase and a solid phase; and / or f2) mixing the separated liquid to a salt, to obtain a resulting liquid composition having a molar concentration of said salt ranging from 0.05 M to 0.5 M, at a temperature ranging from 40° C. to 70° C., to obtain a mixture having a liquid phase and a solid phase; g) precipitating a solid phase from the liquid of step f) for example by a cooling down step of the mixture to a temperature of 30° C. or less; h) separating said solid precipitate from the liquid of step g) said liquid comprising a water-rich liquid phase and an oil-rich liquid phase; i) separating said water-rich liquid phase from said oil-rich liquid phase, j) subjecting said water-rich liquid phase obtained in step i) to one or several membrane filtration(s) to obtain a protein isolate; and k) optionally, drying said protein isolate to obtain a dry protein isolate.

The invention discloses a processing method of high-nutrition germinated brown rice with acidity-induced phytase activity, which comprises the following steps: immersing disinfected brown rice into a citric acid-vitamin C solution with the concentration of 0.5-0.7%, carrying out low-frequency ultrasonic soaking at 25-30 DEG C for 30 minutes, and washing with purified water to obtain pre-infiltrated brown rice; soaking the pre-infiltrated brown rice in purified water at 35-38 DEG C for 6-8 hours until the water absorption rate is greater than or equal to 30%, and adjusting the water temperature to 40 + / -1 DEG C and maintaining for 30 minutes in the last 2 hours of soaking the pre-infiltrated brown rice in the purified water to obtain infiltrated brown rice; and germinating the infiltrated brown rice for 24-36 hours, drying, and sterilizing to obtain the high-nutrition germinated brown rice. Citric acid is utilized to induce and activate the activity of phytase in brown rice, the degradation rate of phytic acid in brown rice is increased, and the absorption rate of trace elements is increased.

The present application relates to a phytase variant and uses thereof.

The invention discloses a phytase-pullulan hydrolase fusion protein Y-L4-T and application thereof, and belongs to the technical field of enzyme engineering. The amino acid sequence of the phytase-pullulan hydrolase fusion protein Y-L4-T disclosed by the invention is as shown in SEQ ID NO. 46. The phytase-pullulan hydrolase fusion protein Y-L4-T is constructed, the phytase activity of the fusion protein is basically consistent with that of phytase YiAPPA, and the amylase activity of the fusion protein is remarkably higher than that of pullulan hydrolase TK-PUL. The wheat bread treated by the fusion protein is obviously increased in specific volume, obviously reduced in hardness and obviously improved in elasticity; the increase of the hardness and the reduction of the elasticity of the wheat bread in the storage period can be effectively relieved. The fusion protein Y-L4-T can effectively improve the quality of bread and prolong the shelf life of the bread, and has a good application prospect in the field of baking.

The application discloses a phytase-pullulanase fusion protein Y-L4-T and application thereof, and belongs to the technical field of enzyme engineering. The amino acid sequence of the phytase-pullulanase fusion protein Y-L4-T is shown as SEQ ID NO. 46. The application constructs a phytase-pullulanase fusion protein Y-L4-T, the phytase activity of the fusion protein is basically consistent with that of phytase YiAPPA, and the amylase activity is significantly higher than that of pullulanase TK-PUL. After the wheat bread is treated by the fusion protein, the specific volume of the wheat bread is obviously increased, the hardness is obviously reduced, and the elasticity is obviously improved; and the increase of the hardness and the reduction of the elasticity of the wheat bread during storage can be effectively alleviated. The fusion protein Y-L4-T can effectively improve the bread quality and prolong the shelf life of the bread, and has a good application prospect in the baking field.

The application discloses a processing method of high-nutrition germinated brown rice with acid-induced phytase activity, and comprises the following steps: immersing disinfected brown rice into a citric acid-vitamin C solution with a concentration of 0.5-0.7 %, and then performing low-frequency ultrasonic soaking at 25-30 DEG C for 30 min, and then cleaning the brown rice with purified water to obtain pre-soaked brown rice; immersing the pre-soaked brown rice into purified water at 35-38 DEG C, and then soaking for 6-8 h until the water absorption rate is greater than or equal to 30 %, wherein the water temperature is adjusted to 40 DEG C plus or minus 1 DEG C in the last 2 h of the soaking, and then maintained for 30 min to obtain soaked brown rice; and drying and sterilizing the soaked brown rice after germination for 24-36 h to obtain high-nutrition germinated brown rice. The application utilizes citric acid to induce and activate phytase activity in the brown rice, thereby improving the phytate degradation rate of the brown rice and the absorption rate of trace elements.

The present invention relates to a fish feed or fish feed additive comprising one or more polypeptides having a phytase activity, wherein said phytase activity comprising EC: 3.1.3.26 (i.e., 4-phytase) and / or EC 3.1.3.8 (i.e., 3-phytase) and / or EC 3.1.3.72 (i.e., 5-phytase) phytase activity, wherein said phytase activity is dosed at a level which is at least 10% less than a efficient (e.g., optimal) dose of phytase activity configured for maximum fish growth response. The present invention relates to a method for reducing phosphorus secretion and / or excretion by a fish, said method comprising: administering the animal feed or animal feed additive according to any one of preceding claims.