The invention discloses a method for preparing a microorganism fermentation feed. The method comprises the main technological processes as follows: respectively culturing Candida utilis, Geotrichum candidum and Streptococcus faecalis with molasses in combination with a liquid microorganism culture medium as the main raw materials; mixing the Candida utilis culture solution, the Geotrichum candidum culture solution and the Streptococcus faecalis culture solution at a certain ratio to obtain a mixed bacteria solution; subjecting to solid fermentation in a semi-solid fermentation culture medium including molasses liquefied liquid, corn coat, vegetable meal, cotton seed meal, wheat bran, soybean meal and apple pomace; baking at a low temperature when the fermentation is finished; and pulverizing to obtain the microorganism fermentation feed. In the microorganism fermentation feed, the viable count of beneficial microorganism is larger than 1*10<9>cfu/g, and the phytase activity is higher than 1,400IU/kg. The feed has the functions of supplementing the number of milk cow (sheep) rumen microorganism, regulating rumen fermentation function, and improving feed conversion and utilization ratio.