Compound microbial fertilizer and preparation method thereof

A technology of compound microorganisms and microorganisms, which is applied in the field of compound microbial fertilizers and its preparation, can solve the problems of pesticide residue toxicity, unreasonableness, soil compaction, etc., and achieve the effect of improving the quality of agricultural products, fully complementary functions, and promoting plant growth

Inactive Publication Date: 2016-06-15
昆明保腾生化技术有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, excessive and unreasonable, timely, and symptomatic use of fertilizers and medications, and low utilization rates of fertilizers and pesticides, have brought about soil compaction, acidification, pesticide residue toxicity, increased resistance (resistance) of diseases and inse

Method used

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  • Compound microbial fertilizer and preparation method thereof
  • Compound microbial fertilizer and preparation method thereof
  • Compound microbial fertilizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1: bacillus atrophaeus ( Bacillus atrophaeus ) Determination of BsR05's ability to antagonize plant pathogenic bacteria

[0038] Botrytis cinerea (Botrytis cinerea Botrytis cinerea ), red rot (Fusarium moniliformis Fusarium moniliforme Sheld), cotton anthracnose Colletotrichumgossypii Soutllw.), wheat leaf blight (Alternaria Alternaria alternata ), wheat root rot (Helmintis elongatum Helminthosporium sativum ), Phytophthora infestans (Phytophthora Botrytis infestans Montagne) with a total of 6 pathogenic bacteria as indicator bacteria. Use the PDA plate confrontation growth method to connect the strains entering the primary screening on the 4 corner points 3cm away from the center of the plate, and move an agar block of pathogenic bacteria with a diameter of 0.3cm into the center of the plate at the same time. , placed in an incubator at 25°C for 3-5 days, please refer to the accompanying drawings for the observation results Figure 1-6 , in the f...

Embodiment 2

[0040] Embodiment 2: bacillus atrophaeus ( Bacillus atrophaeus ) Preparation of BsR05 bacterial liquid

[0041] The liquid fermentation of Paenibacillus polymyxa PB-2 is carried out in the following steps:

[0042] (1) Incline strains: use solid LB medium, inoculate the test tube strains in the test tube culture medium, and cultivate at a constant temperature of 30°C for 36 hours.

[0043] (2) Shake flask strains: using liquid culture medium, inoculate a single colony of slant strains in liquid LB medium and culture on a shaker at a constant temperature of 30°C for 18 hours.

[0044] (3) Liquid fermentation: medium, 2% corn flour, 6% soybean meal, 0.2% (NH 4 ) 2 SO 4 、KH 2 PO4.2H 2 O0.2%, K 2 HPO 4 .2H 2 O0.4%, FeSO 4 ·5H 2 O0.05%, CaCO 3 0.3%, sterilized at 121°C for 20 minutes, inoculated the seeds of (2) in a 10-liter fermenter (1% inoculum), cultivated at 30°C, and the ratio of air flow per minute to the volume of the fermenter was 1.2-1.5: 1. The stirring spe...

Embodiment 3

[0045] Embodiment 3: the preparation of Trichoderma LTR-2 spore suspension

[0046] The design of the technological process follows the liquid-solid two-phase method in order to obtain a large number of Trichoderma conidia.

[0047] process flow

[0048] Inclined seed → eggplant bottle seed → liquid seed → liquid fermentation → inoculation of solid medium → solid culture chamber cultivation → mycelium growth → conidia production

[0049] Process conditions

[0050] Inclined seeds: PDA medium, cultured at 28°C, stored in a refrigerator at 4°C, the hyphae and conidia are required to be plump and free of miscellaneous bacteria.

[0051] Eggplant bottle seeds: PDA medium, cultivated at 28°C, 3-4 days old, no liquid water on the surface of the colony, plump mycelium and conidia, and no contamination by miscellaneous bacteria.

[0052] Liquid seeds: Wash the seeds of 1-2 eggplant bottles with sterilized water, inoculate them in a 500-liter seed tank, and cultivate them at 30°C. T...

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Abstract

The invention discloses compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared from Bacillus atrophaeus and trichoderma which are taken as main active components, and can be used for controlling fungal diseases such as vegetable gray mold and the like in the field. The Bacillus atrophaeus in the compound microbial fertilizer has an inhibiting action on various plant pathogenic fungi, and meanwhile, the trichoderma LTR-2 has phytase activity, so that the compound microbial fertilizer can be used for controlling various plant diseases caused by the plant pathogenic fungi, has the functions of increasing utilization of phosphorus in soil by plants, improving the soil structure, promoting the plant growth and the like, and is multifunctional.

Description

technical field [0001] The invention relates to the technical field of microbial fertilizers, in particular to a composite microbial fertilizer and a preparation method thereof. The composite microbial fertilizer uses Trichoderma and Bacillus atrophaeus as main active ingredients, and can be used in the field to treat fungal diseases such as vegetable gray mold prevention and treatment. Background technique [0002] Microbial fertilizers are fertilizers prepared from one or several living cells of beneficial microorganisms. At present, common microbial fertilizers include rhizobia, nitrogen-fixing bacteria, silicon-dissolving bacteria (potassium bacteria), phosphorus bacteria, antibiotics, and compound bacteria. Microbial fertilizer is a product in which the life activities of microorganisms cause crops to obtain specific fertilizer effects, and it is a type of fertilizer used in agricultural production. [0003] Guaranteeing national food security and effective supply of ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05G3/00C12R1/07C12R1/885
CPCC12N1/20C05G3/00C12N1/14
Inventor 李纪顺张廷金扈进冬施罗丽李秀军杨合同陈凯魏艳丽王贻莲
Owner 昆明保腾生化技术有限公司
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