64 results about "Paenibacillus" patented technology

The invention provides a high-activity chitinase mutant and application thereof in degradation of crustacean waste, and belongs to the technical field of enzyme engineering and agricultural waste conversion. According to the invention, chitinase PbChi from Paenibacillus barengltzii is subjected to molecular modification, and a plurality of chitinase mutants with high activity are successfully constructed by increasing the flexibility of a connexin, changing steric hindrance at a substrate catalytic crack inlet and changing catalytic residues in a beta sheet layer in a catalytic structural domain through calculation design and experimental verification; the chitinase mutant can effectively degrade shrimp shell meal, so that carapace waste is effectively utilized, and the chitinase mutant has a wide application prospect in the biological feed industry.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention relates to the technical field of plant disease prevention and control, in particular to a method for rapidly screening biocontrol bacteria of Candidatus Liberobacter asiaticum and application of the method. The rapid screening method comprises the following steps: mixing an Lcr-GFP bacterial suspension and a to-be-screened strain CFS according to a volume ratio of 1: 1-17: 3, incubating for more than 3 h, detecting fluorescence intensity when the excitation wavelength is 474-490 nm and the emission wavelength is 533-549 nm, and screening the biocontrol bacteria with the fluorescence quenching rate being greater than or equal to 10%. According to the present invention, the screened bacterial strain Paenibacillus edorifer has the inhibition effect on Lcr-GFP, and has the inhibition effect on Clas colonization in the in vivo detection of HLB infected plants, such that the Lcr-GFP inhibition effect can be provided, and the Lcr-GFP inhibition effect can be provided; therefore, the method for rapidly screening the biocontrol bacteria can effectively identify and screen the biocontrol bacteria with remarkable inhibitory activity on Clas, and has the biocontrol potential of HLB prevention and control.

This invention discloses a method, system, equipment, and medium for activating and promoting the release of humic acid from silkworm excrement. The method includes: mechanochemical synergistic crystal-breaking pretreatment, i.e., dry ball milling to destroy the physical encapsulation structure and induce a local alkaline microenvironment under chemical solvent-free conditions; stepwise enzymatic hydrolysis bridging activation, utilizing the acid buffering properties of silkworm excrement, sequentially adding uricase, cellulase / hemicellulase, and laccase or manganese peroxidase to form a natural pH fluctuation curve; directed microbial induction and release promotion, inoculating with Bacillus amyloliquefaciens and Bacillus lentigines for liquid fermentation to achieve metal ion chelation and surface charge regulation; and finally, low-temperature drying and molding to obtain the product. This invention also discloses the system, computer equipment, and storage medium for implementing this method. This invention does not require the addition of external acids, alkalis, or chemical chelating agents, shortens the total cycle to 2-4 days, and the obtained product has a high water-soluble humic acid content, which enhances the resistance to refixation after application to the soil.

This invention discloses an alkali-resistant xanthan gum side-chain lyase mutant and its preparation method, which is effective against alkali-resistant xanthan gum from Bacillus subtilis (Bacillus spp.). Paenibacillus nanensis Site-directed mutagenesis was performed on wild-type xanthan gum side-chain lyase to obtain alkali-resistant xanthan gum side-chain lyase mutants. The site-directed mutagenesis included at least one of Y260V, T387A, and I422S. The xanthan gum side-chain lyase mutants of this invention showed significantly increased enzyme activity compared to the original enzyme after treatment with an alkaline buffer system at 40°C, with the three mutants Y260V / T387A / I422S showing the greatest increase. These mutants are suitable for large-scale degradation of xanthan gum side chains under alkaline conditions, possessing significant social and economic benefits and broad application prospects.

The present application belongs to the technical field of tobacco planting, and provides a whole-cycle planting method for improving quality and increasing yield of tobacco, comprising the following steps: (1) seed treatment: after cleaning the seeds with water, the seeds are soaked in an elicitor composed of gibberellin with a concentration of 50-1000 mg / L and 10-15 mL / L of Bacillus polymyxa dgnkzx004 fungicide fermentation liquor, then subjected to light treatment, and washed and dried; (2) seedling raising: mixed bacteria cow manure fermentation products are added to the seedling raising substrate, and Bacillus polymyxa dgnkzx004 fermentation liquor is applied for seedling raising; (3) transplanting: the tobacco seedlings are transplanted, and the mixed bacteria cow manure fermentation products are used as fertilizer for fertilization. The present application promotes seed germination rate, improves seedling emergence rate and seedling survival rate, and can significantly improve the growth potential of tobacco root system and the yield of tobacco, and significantly reduce the arsenic content in tobacco leaves.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a bacillus PY4 strain capable of efficiently antagonizing various phytopathogens and application of the bacillus PY4 strain, and belongs to the technical field of biological prevention and control of plant diseases. The strain has stable morphological characteristics, and is identified according to a 16S rRNA (ribosomal Ribonucleic Acid) sequence, and the strain is a Paenibacillus bacterium. An in-vitro confrontation test proves that the PY4 can effectively inhibit pathogenic fungi such as sclerotinia sclerotiorum, fusarium graminearum, pseudocauda sp, alternaria alternata and sclerotium rolfsii. Field application results show that the PY4 has a good prevention and treatment effect on sclerotinia rot of colza. The invention further provides a biocontrol agent based on the strain and an application method of the biocontrol agent. The biocontrol agent can be used for green prevention and control of soil-borne and leaf-borne fungal diseases of crops.

This invention discloses a strain of *Bacillus paramycoides* and its application in selenium oxidation, belonging to the field of agricultural microbiology. A strain of *Bacillus paramycoides* H1 was isolated from the soil of a selenium-nutrition experimental field at Huazhong Agricultural University and deposited at the China Center for Type Culture Collection (CCTCC) on June 27, 2023, with accession number CCTCCNO: M 20231107. Experimental verification showed that this strain can simultaneously oxidize elemental selenium and organic selenium. This invention utilizes this strain to convert organic and elemental selenium into water-soluble selenium that promotes plant absorption, laying a scientific foundation for the preparation of safe and low-cost plant selenium-enriching agents and selenium oxidants. This is of great significance for the research and agricultural cultivation of selenium conversion model strains.

This invention belongs to the field of bio-fermentation technology and relates to a strain of Bacillus videmannii SXZY-01 and its applications, including: isolating and screening a strain of Bacillus videmannii capable of producing tanninase from the surface of tobacco. Bacillus wiedmannii The strain SXZY-01, with accession number CCTCC NO:M 2025151, was used to ferment a biological agent containing SXZY-01. After fermentation optimization, the tannin enzyme activity reached 40.98 U / mL. This invention applies a high-enzyme-activity solution to tobacco leaf fermentation and optimizes the fermentation process, achieving efficient degradation of tannins, significantly improving the sensory quality of tobacco, reducing the bitter aftertaste, and demonstrating outstanding technical effects. It provides a green and environmentally friendly solution for upgrading tobacco quality and is in line with the industry's high-quality development direction.

The invention discloses an alkali-resistant xanthan gum side chain lyase mutant and a preparation method thereof, the alkali-resistant xanthan gum side chain lyase mutant is obtained by carrying out site-specific mutagenesis on wild type xanthan gum side chain lyase from paenibacillus nanensis, and the mutation site of the site-specific mutagenesis comprises at least one of Y260V, T387A and I422S. According to the xanthan gum side chain lyase mutant disclosed by the invention, the enzyme activity of the mutant treated by using an alkaline buffer system is obviously improved compared with that of an original enzyme at 40 DEG C, and the enzyme activity is improved to the greatest extent by using the three mutants Y260V / T387A / I422S, so that the xanthan gum side chain lyase mutant is suitable for large-scale degradation of a xanthan gum side chain under an alkaline condition, has important social and economic benefits and is wide in application prospect.

The invention relates to the field of vegetable planting, in particular to a method for applying phosphate solubilizing bacteria extracted from tea garden soil to leaf lettuce growth promotion. The invention relates to a method for applying phosphate solubilizing bacteria extracted from tea garden soil to growth promotion of leaf lettuce, which comprises the following steps: selecting an activated strain, placing the strain in a triangular flask filled with an LB liquid culture medium, culturing on a reciprocating shaking table until bacterial liquid is slightly turbid, measuring the absorbance value of bacterial suspension at the wavelength of 600nm by using a spectrophotometer, measuring the OD600 value of the bacterial suspension to 0.7-0.9, and calculating the content of the phosphate solubilizing bacteria. A pipette is used for sucking bacterial liquid, 0.8% normal saline is added for constant volume, and then lettuce root irrigation treatment is carried out; the bacterial strain is one of bacillus velezensis, bacillus thuringensis, Burkholderia, Burkholderia, and Paenibacillus lentigera, and the bacterial strain is one of bacillus velezensis, bacillus thuringensis, Burkholderia, Burkholderia, and Paenibacillus lentigera.

The application discloses a Paenibacillus sp. NGMCC 1.200843 derived from rhinoceros feces, belongs to a new discovery in the technical field of microorganisms, and is identified as a new species of Paenibacillus, numbered as NGMCC 1.200843=CGMCC 1.64763T, and has been registered and preserved in the China General Microbiological Culture Collection Center. The strain can form a larger transparent circle on a specific culture medium, indicating that the strain has strong phosphorus and starch degradation capacity. Under the current situation of soil fertility decline and fertilizer abuse, the strain provides a high-quality strain resource for the development of microbial fertilizers with organic phosphorus degradation function, and helps to promote the development of microbial fertilizers. The hydrolytic enzymes produced by the strain have wide application potential in the industrial fields of food, fermentation and pharmaceuticals. In particular, amylase can hydrolyze starch to produce various products, and has important significance for the biotechnology industry, and the application range covers multiple industries such as food, fermentation, textile and papermaking. In a word, the application provides a new resource for the development of microbial fertilizers and industrial enzyme preparations.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

The invention belongs to the technical field of microorganisms, discloses a paenibacillus zeae strain and application of the paenibacillus zeae strain in prevention and treatment of plant diseases, and particularly relates to application of the paenibacillus zeae strain in prevention and treatment of lycium barbarum root rot. The paenibacillus zeae is separated from rhizosphere soil of a Chinese wolfberry plant suffering from root rot, the strain number is R2, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36137. The invention further discloses a preparation method of the paenibacillus zeae. The strain can inhibit the growth of various fungal pathogenic bacteria including lycium barbarum root rot, can generate cellulase and amylase, and can induce the expression of defensive genes such as PR1 and CAT2 of plants. Pot experiments show that the control effect of the strain on lycium barbarum root rot reaches 66.66%, the disease morbidity and disease index can be remarkably reduced, and effective biological resources are provided for green control of plant diseases, especially the lycium barbarum root rot.

The application belongs to the technical field of bioactive substance preparation and agricultural application, and discloses a beta-amino butyric acid, a whole cell conversion preparation method thereof and application thereof. The method uses Paenibacillus halophilus as a catalyst to perform biological conversion in a reaction system containing a substrate, so as to obtain the beta-amino butyric acid. The method has mild reaction conditions, the bacterial cells can be repeatedly used, the conversion rate is high, and the green, efficient and low-cost production of the beta-amino butyric acid is realized. The application also provides application of the beta-amino butyric acid prepared by the above method in improving salt stress resistance of crops and in plant disease control as a pesticide synergist. Experiments show that the beta-amino butyric acid can significantly alleviate the inhibition of salt stress on the growth of wheat, and improve the chlorophyll content and biomass accumulation; the beta-amino butyric acid has a synergistic effect on grape diseases when used in combination with anilazodifen and fluoroacetamide, and meanwhile, the fruit quality and storage resistance are improved.

The application belongs to the field of application of agricultural microorganism and biological control technology, and particularly relates to a bacillus chagamensis SY7-28, a microbial agent, a microbial pesticide and application. Fictibacillus barbaricus The bacillus chagamensis (Bacillus chagamensis) SY7-28 is preserved in the China General Microbiological Culture Collection Center on June 27, 2025, and the strain preservation number is CGMCC No. 35029. The bacillus chagamensis SY7-28, the microbial agent and the microbial pesticide disclosed by the application not only have a control effect on tomato bacterial wilt, but also have a significant growth promotion effect.

The application discloses a kind of organic fertilizer based on biomass fermentation and its preparation method and application, belong to biological organic fertilizer technical field.The organic fertilizer based on biomass fermentation of the application includes the following raw materials: chicken manure 40-50 parts, rapeseed cake 20-30 parts, seaweed powder 15-20 parts, corn straw 10-15 parts, EM bacterial liquid 10-15 parts, compound microbial inoculant 6-10 parts, Rhizoma Cyperi 8-16 parts, mulberry branch 4-8 parts, water hyacinth 12-18 parts, mushroom residue 5-8 parts, wherein the compound microbial inoculant is composed of jelly-like bacillus, sand on flavobacterium, rhizosphere oligotrophic single-cell bacteria, which can quickly decompose organic matter, accelerate the production of small molecules, and be directly absorbed by plants and produce a variety of active substances during fermentation.The organic fertilizer of the application has a synergistic effect of multiple substances, significantly promotes photosynthesis of plants, improves nutrient production and transportation, effectively promotes growth of Chinese cabbage and improves quality of Chinese cabbage.

This invention discloses a multifunctional Paenibacillus polymyxa with straw-degrading, broad-spectrum antibacterial, and highly effective insecticidal activities, and its applications, belonging to the field of agricultural microbiology. The strain provided by this invention is Paenibacillus polymyxa BMB-Pp0021, with the strain preservation number CCTCC NO.M20241951. This strain not only exhibits broad-spectrum antibacterial activity but also highly effective insecticidal properties, and simultaneously possesses the ability to efficiently degrade straw, especially under low-temperature conditions (7-day weight loss rate of 50.20%). These multifunctional characteristics give it enormous application potential in agriculture and industry.

The application provides a strain of mineralized soil organic phosphorus, Domibacillus indicus D99, and application of the strain in soil organic phosphorus mineralization, growth promotion of phosphorus solubilizing bacteria and plant growth promotion. The application first shows the function and role of Domibacillus indicus in soil organic phosphorus mineralization and plant growth promotion, and has important value for development of phosphorus solubilizing bacteria fertilizer and other microbial fertilizers. The strain of Domibacillus indicus D99 provided by the application has the ability of dissolving soil organic phosphorus, the acid phosphatase activity is 17.32, the alkaline phosphatase activity is 1.3, can promote plant growth, and improves the aboveground biomass by 50-115.

The invention provides a saline-alkali soil conditioner and a preparation method thereof. The preparation method comprises the following steps: S1, preparing polystyrene microspheres coated with polyethyleneimine; s2, preparing attapulgite of which the surface is grafted with TEMPO oxidized cellulose, and then carbonizing the attapulgite to obtain a carbon-coated attapulgite composite material; s3, preparing composite aerogel; s4, bacillus pasteurii and azotobacter chroococcum are loaded on the composite aerogel, and the saline-alkali soil conditioner is obtained. The double strains are loaded on the composite aerogel, controlled release and slow release of the strains are achieved, in addition, the two microbial agents serve as dominant flora, breeding of harmful pathogens in soil can be inhibited, soil micro-ecological balance can be adjusted, and a healthy rhizosphere environment is created for follow-up crop planting.

The application discloses a bacillus mycoides BMS-39 and application thereof, and belongs to the technical field of microorganisms and agricultural biological control. Fulvia fulva The strain BMS-39 has a very strong inhibiting effect, the inhibiting rate reaches 92.3% by means of a flat-plate confrontation method, the inhibiting rate of 10% fermentation filtrate reaches 87.6%, the potting prevention effect reaches 87.01% in 14 days, and the field prevention effect reaches 87.81%. Even when the concentration of the bacterial liquid is reduced to 10 6 CFU / mL, the prevention effect of the BMS-39 can still reach 81.46%, which means that the fermentation cost and application cost of the application can be greatly reduced under the premise of meeting the same or even better prevention effect, and the application has significant market competitiveness.

The application discloses an atrazine-degrading strain and application thereof, the strain is strain HB172176, the taxonomic name of the strain HB172176 is Paenibacillus Danchangensis , the preservation number is CGMCC NO.1.16820, and the preservation date is September 9, 2021, and the application of the atrazine-degrading strain in preparation of an atrazine-degrading product is also disclosed. The strain is separated from a mangrove habitat with variable environmental conditions, has atrazine-degrading activity, has wide adaptability to pH and salinity, and can be applied in more environments.

The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a complex microbial inoculant for promoting quality improvement and yield increase of alfalfa as well as a preparation method and application of the complex microbial inoculant. The complex microbial inoculant provided by the invention is prepared from P.aryabhattai 96 and Paenibacillus gramineus H5 according to a specific proportion, and the complex microbial inoculant can assist plants to adapt to a stress environment, increase the biomass and enhance the tolerance and repair ability to pollutants through the mechanisms of nitrogen fixation, phosphorus solubilization and siderophore production.

The invention belongs to the field of agricultural microorganism and waste resource utilization, and discloses a functional complementary flora and cellulase synergistic straw decomposition agent and application thereof. The decomposition agent is prepared by scientifically compounding a complex microbial community consisting of bacillus proteolyticus WYX-67, P.avenae WYX-3 and paenibacillus polysaccharificus WYX-166 with industrial cellulase, and the complex microbial community consists of bacillus proteolyticus WYX-67, P.avenae WYX-3 and paenibacillus polysaccharificus WYX-166. The technical bottlenecks of product inhibition, enzyme activity attenuation, slow start and the like in application of a single preparation are effectively overcome through the'quick start 'effect of exogenous enzyme and'continuous relay' degradation of microbial flora. Test data show that the decomposition rate and the organic carbon mineralization ability of the wheat straw can be remarkably improved, and a brand new technical scheme is provided for efficient and environment-friendly treatment of the straw returned to the farmland.

The invention provides bacillus mojavensis and application thereof. The bacillus mojavensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO. 35674. The bacillus mojavensis has the advantages that the bacillus mojavensis can be used as a bacillus mojavensis strain; the bacillus mojavensis has multiple biological activities: on one hand, phosphorus-solubilizing related metabolites, potassium-solubilizing related metabolites, siderophores and indoleacetic acid can be generated, and meanwhile, the bacillus mojavensis has the activity of 1-aminocyclopropane-1-carboxylic acid deaminase, so that the growth of seedlings of crops such as jujubes, cucumbers and the like can be remarkably promoted; and on the other hand, the strain has broad-spectrum bacteriostatic activity on 16 pathogenic fungi such as jujube fruit black skin pathogen and cucumber root rot pathogen, has excellent prevention and control effects on jujube black skin disease and cucumber root rot, and can effectively regulate and control a cucumber rhizosphere micro-ecological structure and improve crop disease resistance. Besides, the JZBQ1 strain also has remarkable salt-tolerant and drought-resistant characteristics, can stably play the roles of disease prevention, growth promotion and yield increase under complex cultivation conditions such as conventional cultivated land, saline-alkali soil and drought, and has huge application potential. The invention provides a new strain resource for development and application of an efficient biocontrol inoculant, and has important significance for promoting green development of fruits and vegetables and reducing dependence on chemical pesticides.

The invention discloses a functional microbial agent for near-natural recovery of moderately-degraded high-cold grassland as well as a preparation method and application of the functional microbial agent. The composite bacterial agent is prepared by mixing bacterial culture solutions of four bacterial strains, namely bacillus pumilus E-B-2-B05 (CGMCC No. 21387), paenibacillus silage P-B-2-B03 (CGMCC No. 21386), bacillus subtilis P-B-4-SCB23 (CGMCC No. 27084) and bacillus velezensis K-B-5-SCB13 (CGMCC No. 27088) according to the volume ratio of 3: 2.5: 2: 2.5. The invention further discloses a preparation method of the composite bacterial agent. Through the synergistic effect of multiple strains, the complex microbial inoculant can effectively promote plant growth, enhance rhizosphere microbial activity and improve the ecological process of soil, can effectively improve grassland biomass, optimize a plant community structure, enhance competitive advantages of high-quality pasture, and promote moderately-degraded alpine grassland vegetation to recover to a near-natural state. The microbial agent is simple in preparation method, convenient and flexible to apply and environment-friendly, and has good ecological and economic benefits.

This disclosure provides compositions and methods for treating and immunizing insects and insect populations against bacterial diseases. This disclosure further provides compositions and methods for prophylactic immunization of beehives to protect them from larval foulbrood infection. In embodiments, this disclosure further provides methods for prophylactic immunization of beehives to protect them from infection by Bacillus larvae (Bacillus subtilis). Paenibacillus larvae Compositions and methods for causing American foulbrood infection in larvae.

The present invention addresses the problem of providing a microbial material that can also be applied to agricultural crop cultivation in a dry area.　The present invention provides the following endospore-forming bacteria (A) to (C) having plant-growth promoting activity in a dry environment. (A) Either Bacillus thuringiensis TTREp1 strain identified by the accession number NITE BP-04159 or Bacillus altitudinis TCo-SSREp1 strain identified by the accession number NITE BP-04160, or a mutant strain thereof (B) Paenibacillus polysaccharolyticus TP-WSREp1 strain identified by the accession number NITE BP-04163, or a mutant strain thereof (C) either Priestia aryabhattai TTREn1 strain identified by the accession number NITE BP-04165 or Priestia megaterium TC-WSREn1 strain identified by the accession number NITE BP-04166, or a mutant strain thereof