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160 results about "Paenibacillus" patented technology
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Paenibacillus is a genus of facultative anaerobic, endospore-forming bacteria, originally included within the genus Bacillus and then reclassified as a separate genus in 1993. Bacteria belonging to this genus have been detected in a variety of environments, such as: soil, water, rhizosphere, vegetable matter, forage and insect larvae, as well as clinical samples. The name reflects: Latin paene means almost, so the paenibacilli are literally "almost bacilli". The genus includes P. larvae, which is known to cause American foulbrood in honeybees, the P. polymyxa, which is capable of fixing nitrogen, so is used in agriculture and horticulture, the Paenibacillus sp. JDR-2 which is known to be a rich source of chemical agents for biotechnology applications, and pattern-forming strains such as P. vortex and P. dendritiformis discovered in the early 90s, which are known to develop complex colonies with intricate architectures as shown in the pictures...
Mixing and matching TC proteins for pest control
InactiveUS7491698B2High activityEffective control of widerBiocidePeptide/protein ingredientsHeterologousToxin protein
The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from Xenorhabdus, Photorhabdus, and Paenibacillus, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a “stand-alone” TC protein (from Photorhabdus, Xenorhabdus, or Paenibacillus, for example) is enhanced by one or more TC protein “potentiators” derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-alone Xenorhabdus protein toxin as well as a stand-alone Photorhabdus protein toxin. (As one skilled in the art knows, Xenorhabdus toxin proteins tend to be more desirable for controlling lepidopterans while Photorhabdus toxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.
Owner:DOW AGROSCIENCES LLC
Family 44 xyloglucanases
Owner:NOVOZYMES AS
Preparation of microcapsules of viable microbe biopesticide
The invention relates to microcapsules prepared from viable microbes such as pseudomonas, bacillus and paenibacillus serving as main active components, belonging to the technical field of microorganism and biochemistry. The method for preparing microcapsules of viable microbe biopesticide comprises the following steps of: adsorbing microbial fermentation culture solution through an adsorption effect to prepare capsule cores and solidifying the capsule cores, and adding wall materials, so as to prepare a microcapsule preparation. The microcapsule preparation prepared by adopting the method has high content of viable microbes, high storage property and strong high-temperature and ultraviolent resistances, and stable and good prevention and control effect on plant diseases and insect pests.
Owner:成都特普生物科技股份有限公司
Endogenetic polymexa bacillus of plant for prophyiaxis and promoting growth and application thereof
The present invention relates to Paenibacillus polymuxa and its applications, which is from a kind of prevented disease and spurred development plant. It belongs to prevented and controlled disease technology fields of crop. The Jaas cd can enter, propagate and transmit into the body of this plant on the condition that the lower chroma of bacterium, meanwhile, it can promote growth, increase output and prevent disease, especially to prevent passes disease. This Jass cd is widely used in reagent, the price is cheap, manipulation is simple, it does good to prevent field zoology environment, reduce rudimental prison, it suits to Chinese yielding requirement of non-social effects of pollution farm produce.
Owner:JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
A microbial mixed flora that enhances the flavor of liquor brewing
The invention relates to a microbial mixed colony capable of enhancing flavor of brewed white liquor, which comprises live brevibacillus borstelesis, clostridium.sp, bacillus subtilis, paenibacillus, clostridium thermo succinogene and brevibacillus brevis and can be used for brewing white liquor with strong aromatic flavor. The mixed colony is used in the fermentation process in brewing of the white liquor of the strong aromatic flavor to help a saccharifying starter improve the flavor of raw liquor. The mixed colony is capable of degrading cellulose, and can improve the utilization rate of liquor brewing raw materials and increase yield of white liquor. Meanwhile, the mixed colony is proved to have the characteristics of producing beneficial metabolins such as protease, amylase, organic acids, lipase and vitamins, along with ethanol resistance and heat resistance, and therefore enjoys a great advantageous in the brewing environment; and the metabolins are benefit for enhancing the flavor of the white liquor and increasing the nutrients in the white liquor.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Bacteria and method for improving plant health and growth
InactiveUS20160227789A1Improve disease resistanceGood for healthBiocideBacteriaBacillus megateriumBacterial composition
This application describes and claims a method of improving the health and vigor of a plant, comprising administering to the plant an effective amount of a bacterial composition which comprises a botanically compatible vehicle and an isolated bacterial strain selected from the group consisting of Bacillus megaterium PT6, Bacillus subtilis PT26A, Paenibacillus sp. ATY16, and any combination thereof. Improvement in health and vigor is one or more of the following: a) improved resistance to disease; b) improved ability to defend against disease; c) reduction of disease symptoms; d) faster growth; e) improved crop productivity; f) improved crop quality; g) improved seed germination; h) improved seedling emergence; or any combination thereof. Isolated bacterial strains and mixtures of bacteria are provided, as well as compositions comprising the isolated bacterial strains.
Owner:UNIV OF FLORIDA RES FOUNDATION INC
Strain of Paenibacillus jejuni capable of inhibiting anthrax and Fusarium and application thereof
ActiveCN109266589AGood control effectBroad spectrum antibacterialBiocideBacteriaSheath blightFusarium
The invention discloses a strain of Paenibacillus jejuni for inhibiting anthrax and Fusarium and an application thereof. A strain of Paenibacillus jamilae is isolated from the deep sea sediment, the strain was deposited at the General Microbial Center of the China Microbial Culture Collection Management Committee under accession number CGMCC No.16231 on August 8, 2018. The strain has broad-spectrum resistance to many plant pathogens, and has good control effect on fruit tree (citrus, apple, grape) anthracnose caused by Colletotrichum gloeosporioides and vegetable wilt caused by Fusarium oxysporum.
Owner:CHINA KINGDOM AGRITECH QINGDAO
Novel Strains Belonging to the Genus Paenibacillus and Method of Controlling Plant Disease by Using These Strains or Culture Thereof
Novel strains Paenibacillus sp. BS-0048, Paenibacillus sp. BS-0074, Paenibacillus polymyxa BS-0105 and Paenibacillus sp. BS-0277 and Fusaricidin A, Fusaricidin B and novel compounds 3 and 4 produced thereby have an activity of inducing resistance to plant diseases. Thus, they can protect plants from infections with fungi, bacteria, viruses and so on and, as a result, effectively control plant diseases.
Owner:KAKEN PHARMA CO LTD
Method for efficiently preparing D-psicose 3-epimerase and use of D-psicose 3-epimerase
InactiveCN107723307ALow costHigh catalytic efficiencyBacteriaMicroorganism based processesIsozymePsicose
The invention discloses a method for efficiently preparing D-psicose 3-epimerase and a use of the D-psicose 3-epimerase and provides a method for efficiently preparing an enzyme catalyst for catalyzing the same reaction through isozyme combined expression. The method utilizes corynebacterium glutamicum as a host cell to construct a recombinant strain expressed by plasmid dissociation and chromosomal integration, measures D-fructose catalytic efficiency, improves enzyme catalytic efficiency by 2-4 times than that of single expression of D-psicose 3-epimerase through combined expression and hasa conversion ratio of 29% when 70% of fructose is a substrate. The method improves D-psicose production efficiency and is suitable for industrial production of D-psicose. The invention also disclosesa novel use of the D-psicose 3-epimerase in psicose synthesis. The enzyme is derived from Paenibacillus senegalensis, has catalytic activity of about 25 U / mg and can be used to convert D-fructose intoD-psicose.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Composite microbial fertilizer for controlling soil-borne disease and preparation method thereof
InactiveCN105732195AImprove micro-ecological environmentIncrease diversityBio-organic fraction processingAnimal corpse fertilisersBiosphereResource pool
The invention discloses a composite microbial fertilizer for controlling soil-borne disease and a preparation method thereof. The composite microbial fertilizer for controlling soil-borne disease is prepared from the following raw materials in parts by weight: waste livestock / fowl feather, rice bran, dry manure, weathered coal, biochar, a composite microbial inoculant, humic acid, bentonite, potassium sodium tartrate, citric acid, copper sulfate and ferrous sulfate. The composite microbial inoculant is prepared from the following raw materials in parts by weight: Bacillus megaterium, Bacillus mucilagimosus krassilm and Paenibacillus sabinae. The composite microbial fertilizer contains abundant various microbes, and the composite microbial inoculant is added to increase the beneficial bacterium content, so that the composite microbial fertilizer becomes a microbe resource pool. After being applied by drip irrigation and spraying, the composite microbial fertilizer can adjust the phytoedaphon structure, enhance the soil microbe diversity, improve the microecological environment of the soil and reduce the biosphere of the phytopathogen, thereby achieving the goal of controlling soil-borne disease.
Owner:GUANGXI WEILIDA BIOTECH CO LTD
Microbial compound bacteria for preventing and treating citrusyellowshoot and preparation method and application thereof
The invention provides microbial compound bacteria, a preparation method thereof and application of the microbial compound bacteria to prevention and treatment of citrusyellowshoot. The microbial compound bacteria comprise Cribben paenibacillus and bacillus subtilis, the biological preservation serial number of the Cribben paenibacillus is CGMCC NO.7996, and the microbial compound bacteria can be specifically microbial compound bacterium liquid or a microbial compound bacterium agent. The preparation method of the microbial compound bacteria includes the specific steps that the Cribben paenibacillus and the bacillus subtilis are mixed in proportion after being fermented. According to the microbial compound bacteria for preventing and treating citrusyellowshoot and the preparation method and application thereof, procreation and propagation of gram negative bacteria are inhibited in a root filling and leaf surface spraying mode, 'bacteria are controlled through bacteria', harmful bacteria in soil are killed, soil quality is optimized, root and stem phloem sieve tubes can be dredged, the problem of nutrient substance conveying barrier is solved, diseased or infected plant nutrients are recovered, growth of new roots is promoted, tree roots are more developed, tree tubular cells are smoother, yellow leaves of fruit trees are turned green, and healthy new tips grow.
Owner:江西啄木蜂科技有限公司
Microbial flora capable of efficiently degrading PBAT plastic mulching film
InactiveCN108004166AEfficient degradationBacteriaMicroorganism based processesMicroorganismBacillus amyloliquefaciens
The invention provides a microbial flora capable of efficiently degrading a PBAT plastic mulching film. The microbial flora includes brevibacillus borstelesis, paenibacillus, bacillus subtilis, bacillus amyloliquefaciens, sulfur streptococcus and vibrio leeches. The microbial flora capable of efficiently degrading the PBAT plastic mulching film is found for the first time at home and abroad; in the prior art, the physical properties of the PBAT plastic film are researched, and the technology for screening and utilizing the degrading flora are not found. After the flora is acclimatized, on thebasis of weight loss method calculation, the degrading efficiency for the PBAT plastic film can reach 99% or above within 13 d, and after degradation, naked eyes cannot observe residues of the formedPBAT mulching film.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Nitrogen fixing Paenibacillus 1-43 and application thereof
ActiveCN102352332BHas the ability to fix nitrogenBiocidePlant growth regulatorsOrganismMicrobiological culture
The invention provides Paenibacillus sp. strain 1-43 which has nitrogen fixing capability and conservation number of CGMCC (China General Microbiological Culture Collection Center) No. 4965. The strain contains a nitrogenase structure gene nifH, and has the nitrogenase activity of 904.49 nmol C2H4 / (mg protein h)<-1> measured by an acetylene reduction method. The nitrogen fixing Paenibacillus strain 1-43 has an obvious inhibition action on multiple plant pathogenic bacteria; and the nitrogen fixing microorganism agent or biological organic fertilizer prepared from the strain 1-43 has an obvious promotion action on the germination and growth of plant seeds, can be popularized and used in agricultural production, and can effectively reduce the use amount of urea.
Owner:HUBEI FORBON TECH
Paenibacillus fermentation broth and preparation method and application thereof
ActiveCN106148251AEasy to prepareLow equipment requirementsMilk preparationBacteriaFermentation brothLactobacillus plantarum
The invention discloses a paenibacillus fermentation broth and a preparation method and application thereof. The fermentation broth is fermented supernatant obtained through fermental cultivation of paenibacillus CGMCC No.8333. It is proven through tests that the paenibacillus fermentation broth has an effect of promoting growth of lactobacillus plantarum ST-III. The method for preparing the fermentation broth is simple, the equipment requirements are low, and the cost is low. The adopted paenibacillus CGMCC No.8333 comes from foods and is safe and non-toxic, and the supernatant prepared through fermentation can be directly applied to food processing. Besides, the fermentation broth greatly improves application potential of lactobacillus plantarum ST-III in actual product development.
Owner:BRIGHT DAIRY & FOOD
Mixture, dissolving solution and pharmaceutical agent each comprising thermophilic microorganism
ActiveUS9987312B2Good effectReduce in greenhouse gasesMetabolism disorderMicroorganismsPyrococcusGenus Thermus
To provide a mixture, a dissolving solution and a pharmaceutical agent, which contain a thermophilic microorganism to make it possible to regulate a mucous membrane immune system gene cluster and metabolism-related gene clusters of the intestines and the liver. Each of them is prepared by fermentation of an organic material containing a thermophilic microorganism at a temperature of 50° C. or more and 90° C. or less. By being administered to the animal, it regulates at least one of a mucous membrane immune system gene cluster, a metabolism-related gene cluster in the intestines, and a metabolism-related gene cluster in the liver of the animal. The microorganism includes at least one species of the genus Bacillus, Oceanobacillus, Paenibacillus, Anoxybacillus, Lysinibacillus, Methanopyrus, Geogemma, Pyrolobus, Pyrodictium, Hyperthermus, Pyrococcus, Pyrobaculum, Thermococcus, Aeropyrum, Aquifex, Thermotoga, Thermodesulfobacterium, Thermus, Geobacillus, and Thermomyces.
Owner:JAPAN ECO SCI +5
Bacteria strain of antagonistic plant pathogenic bacteria and application of bacteria strain
Owner:BEIJING FORESTRY UNIVERSITY +1
Solid bacterial fertilizer for remedying petroleum polluted saline alkali soil and preparation method of solid bacterial fertilizer
InactiveCN104649848AEasy to makeEasy to getOrganic fertilisersFertilizer mixturesBacillus licheniformisNutritional status
The invention discloses a solid bacterial fertilizer for remedying petroleum polluted saline alkali soil. The solid bacterial fertilizer consists of a flora mixed liquid, a high-salt beef extract peptone crude oil culture medium and a solid medium, wherein the flora mixed liquid consists of mixed bacillus, paenibacillus, bacillus coagulans, bacillus licheniformis and enterobacter; the flora mixed liquid is inoculated in the high-salt beef extract peptone crude oil culture medium for culture and enrichment; the solid medium consists of high pressure sterilized wheat bran and saw dust. The solid bacterial fertilizer has the advantages of simple preparation and easiness in obtaining of materials; when petroleum polluted saline alkali soil is remedied by using the solid bacterial fertilizer, the treatment cost is low, the ventilation property and the nutrition condition of soil can be improved, the secondary pollution to environment is prevented, and the degradation efficiency of petroleum hydrocarbon in saline alkali soil can be remarkably improved; after 60 days of remediation period, the salt content of soil is 6.1g / kg, and the petroleum degradation rate under the high salt condition can be up to 46.0%.
Owner:TIANJIN UNIVERSITY OF TECHNOLOGY
Complex flora for preventing and treating peanut root rot and application thereof
PendingCN111763643AEnhancement of rhizosphere colonizationThe disease prevention effect is stableBiocideBacteriaBiotechnologyEcological environment
The invention provides a complex flora for preventing and treating peanut root rot and an application thereof, and belongs to the technical field of soil-borne disease prevention and treatment. The complex flora comprises pantoea, pseudobacillus, enterobacter, paenibacillus, sporosarcina, bacillus and pseudomonas aeruginosa. The pantoea, the pseudobacillus, the enterobacter, the paenibacillus, thesporosarcina, the bacillus and the pseudomonas aeruginosa in the complex flora have a synergistic interaction effect on inhibition of pathogenic fungi; compared with a single strain, the complex flora has the advantages that the rhizosphere colonization capacity is obviously improved, and the rhizosphere micro-ecological environment can be effectively optimized. The complex flora is inoculated topeanut roots, so that the peanut roots can be effectively prevented from being infected by fusarium oxysporum, and the health of the peanut roots is protected.
Owner:INST OF SOIL SCI CHINESE ACAD OF SCI
Paenibacillus and its application in water body purification
ActiveCN106399176AMeet growth needsAdaptableBacteriaWater contaminantsWater qualityBacillus aryabhattai
The invention relates to a water body purifying microorganism, and particularly discloses a paenibacillus YE-1 and its application. The paenibacillus YE-1 is preserved at China General Microbiological Culture Collection Center, the preservation date is August 4, 2016 and the preservation number is CGMCC No. 12814. The paenibacillus YE-1 can be used for effectively absorbing degrading organic matters in black and odorous waste in a biological oxidation reactor, and purifying water quality. Furthermore, since a distributed photovoltaic system supplies power energy to an aeration device of the biological oxidation reactor, the technical flow needs not additional power energy, the operation cost is low, and the treatment is effective and stable.
Owner:重庆华悦生态环境工程研究院有限公司
Mixed bacterial colony microbial preparation and application thereof in treatment of wastewater containing nitrate nitrogen
The invention discloses a mixed bacterial colony microbial preparation which is a bacterial suspension consisting of paenibacillus, pseudomonas stutzeri, pseudomonas pseudoalcaligenes and pseudomonas oleovoran, wherein the viable count of paenibacillus is more than 1.69*10<10>CFU / Ml, the viable count of pseudomonas stutzeri is more than 2.35*10<10>CFU / Ml, the viable count of pseudomonas pseudoalcaligenes is more than 2.18*10<10>CFU / Ml, and the viable count of pseudomonas oleovoran is more than 2.26*10<10>CFU / Ml. The mixed bacterial colony microbial preparation can be used for treating wastewater containing nitrate nitrogen, and the specific application method comprises the steps of regulating the pH value of the wastewater containing nitrate nitrogen to 7.0-7.2, then inoculating the wastewater containing nitrate nitrogen with the mixed bacterial colony microbial preparation according to the inoculation amount of 1-10%, and performing static culture at 30-35 DEG C. The denitrification effect can be obvious after 4 days culture and complete denitrification can be realized.
Owner:SHANDONG UNIV
Soil paenibacillus and application thereof
InactiveCN106399199AUnique in natureStable in natureBacteriaMicroorganism based processesHigh concentrationBacillus aryabhattai
The invention discloses soil paenibacillus and application thereof. The soil paenibacillus is soil paenibacillus NUST16, and the preservation number is CCTCC No.M2016542. According to the soil paenibacillus, the strain is unique in property, exopolysaccharide can be produced, the yield can reach 15 g / L, and the produced exopolysaccharide is easy to separate and purify and unique in property, can be directly dissolved in cold water and regulate the rheological property of an aqueous solution, has the significant effect of increasing the viscosity of the solution, can resist high-concentration Na<+>, K<+>, Ca<2+> and Mg<2+> in the solution and increase the viscosity of the solution by multiple times through simple heating-cooling treatment, is stable in property and can form thermo reversible gel.
Owner:NANJING UNIV OF SCI & TECH
Pesticidally Active Proteins and Polynucleotides Obtainable from Paenibacillus Species
The subject invention provides unique biological alternatives for pest control. More specifically, the present invention relates to novel pesticidal proteins, novel sources of pesticidal proteins, polynucleotides that encode such toxins, and to methods of using these toxins to control insects and other plant pests. The subject invention relates to the surprising discovery that Paenibacillus species, and proteins therefrom, have toxicity to lepidopterans. There have been no known reports of a Paenibacillus species, strain, or protein having toxicity to lepidopterans. This is also the first known example of a Paenibacillus Cry protein that is toxic to lepidopterans. Furthermore, this is the first known report of a Paenibacillus having toxin complex (TC)-like proteins. The DAS1529 isolate disclosed here is also the first known example of a natural bacterium that produces both a Cry toxin and TC proteins. The subject invention also relates to new classes of Cry and TC proteins that are pesticidally active.
Owner:DOW AGROSCIENCES LLC
Beta-N-acetylglucosaminidase of Paenibacillus barungensis, encode gene thereof and application of beta-N-acetylglucosaminidase
ActiveCN109182303AGood enzymatic propertiesIncreased specific enzyme activityBacteriaMicroorganism based processesChitinaseThermal stability
The invention discloses a Beta-N-acetylglucosaminidase of Paenibacillus barungensis, an encode gene thereof and an application of beta-N-acetylglucosaminidase. The provided Beta-N-acetylglucosaminidase PbNag39 is excellent in the enzymatic property, and has a specific activity against chitosan is 28.3 U / mg, and the final products of hydrolysis of chitosan oligosaccharides are N-Acetyl glucosamine.The Beta-N-Acetylglucosaminidase and chitinase can hydrolyze chitin powder synergistically and obtain N-Acetyl glucosamine. The Beta-N-acetylglucosaminidase PbNag39 has the characteristics of high specific activity, good thermal stability and excellent hydrolysis characteristics. It can stabilize and play a catalytic role in a wide range of pH, and has important application value in chitin conversion.
Owner:CHINA AGRI UNIV
Mixing and matching tc proteins for pest control
ActiveUS20090221501A1High activityEffective control of widerBiocidePeptide/protein ingredientsHeterologousPhotorhabdus species
Owner:DOW AGROSCIENCES LLC
Compound microbial preparation for preventing brown spot of Cassava and preparing method thereof
InactiveCN106332905AImprove efficacyStrong targetingBiocidePlant growth regulatorsBacillus licheniformisSecondary metabolite
The invention discloses a compound microbial preparation for preventing brown spot of Cassava and a preparing method thereof. The raw materials of the compound microbial preparation comprises fermentation liquor of streptomyces Durham, fermentation liquor of streptomyces Russa, fermentation liquor of mud Bacillus licheniformis, fermentation liquor of Paenibacillus Barcelona, and fermentation liquor of Trichoderma aggression. According to the compound microbial preparation for preventing brown spot of Cassava, cultures can make full use of antibiosis, competition, hyperparasitism, bacteriolysis of interspecific or intraspecific microorganism; and through secondary metabolite, Trichoderma is induced to generate disease resistance, which can produce multiple antibiotics such as durhamycin, antifungal pentaene antibiotics, and antibiotics which can produce antifungal tetraene; russa mycin is used to prohibit pathogenic bacteria in brown spot of Cassava, and improves disease preventing effect of Cassava. The Compound microbial preparation for preventing brown spot of Cassava is environmental friendly and safe to people and livestock, and can not easily generate drug resistance; meanwhile, the preparing method is simple in procedures, low in cost and easy in using.
Owner:FOSHAN YANHUI BIOTECH CO LTD
High-yield paenibacillus amyloliquefaciens csuft F14 for chitin deacetylase and its application
InactiveCN101544958ASimple cultivation conditionsBreed fastBacteriaMicroorganism based processesMicrobiologyChitin deacetylase
The present invention discloses high-yield paenibacillus amyloliquefaciens csuft F14 for chitin deacetylase and the application of the paenibacillus amyloliquefaciens csuft F14. The preservation entity of the strain is CCTCC, and the preservation number is CCTCC M 209062. The advantage of the present invention is: the strain can be cultured simply and has high enzyme activity when it is used to produce chitin deacetylase, and is favorable for development and utilization of chitin deacetylase, and has excellent application prospects.
Owner:CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
Thermostable chitosanase
InactiveUS20130337541A1Easy to useReduce molecular weightSugar derivativesBacteriaLow molecular weight chitosanCHITOSAN OLIGOSACCHARIDE
The present application concerns a thermostable chitosanase, originally identified in Paenibacillus sp., having an optimal temperature of about 80° C. when measured at a reaction time of about 10 minutes. Also contemplated are variants of this chitosanase (sharing more than 81% identity) as well as fragments thereof. The present application further concerns nucleic acid molecules encoding the chitosanase, vector comprising them as well as host cell expressing them. Methods of producing the thermostable chitosanase as well as using it for generating low molecular weight chitosan and chitosan oligosaccharides are also disclosed.
Owner:SCOPRA SCI & GENIE SEC
Paenibacillus damxungensis sp.nov. CGMCC No.8333 chymosin and preparation method
InactiveCN104911167AIncrease enzyme activityHigh live recoveryMicroorganism based processesPeptidasesChymosinFermentation broth
The invention discloses a paenibacillus damxungensis sp.nov. CGMCC No.8333 chymosin and a preparation method. The method comprises the following steps: (1) centrifuging fermentation broth of which the preservation number is CGMCC No.8333 to obtain fermenting supernate; (2) mixing the fermenting supernate and ammonium sulfate until the saturability of the ammonium sulfate in the fermenting liquid is 50-75% so as to obtain a mixed solution, standing the mixed solution for 0-5 hours at 2-6 DEG C, and centrifugally collecting a precipitate; (3) dissolving the precipitate centrifugally to obtain the supernate. According to the method provided by the invention, the steps of a preparation process of chymosin can be greatly simplified; furthermore, compared with a method of extracting chymosin by using ethanol, the production cost can be greatly saved, the method is convenient and effective to operate, safe and reliable to use, and is beneficial for industrial production.
Owner:BRIGHT DAIRY & FOOD
Paenibacillus peoriae and application thereof
The invention relates to paenibacillus and application thereof, in particular to Paenibacillus peoriae and application thereof. The Paenibacillus peoriae is deposited to the Guangdong MicrobiologicalCulture Collection Centeron on November 16, 2018, and the preservation number is GDMCC No.60482. The Paenibacillus peoriae has a good prevention and treatment effect on pathogenic fungi and bacteria,especially can be used for inhibiting rhizoctonia solani and rastonia solanacearum, and has the advantages of high growth speed, simple nutrition demand, high heat resistance and stress resistance andthe like; and the Paenibacillus peoriae is safe for human and livestock, poisonless and harmless, environmentally friendly and free of pollution, and the pathogenic bacteria are not prone to producing drug resistance, so that the requirements of people for green food are met, and guarantee for the sustainable development of agriculture is provided.
Owner:MOON (GUANGZHOU) BIOTECH CO LTD
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