101 results about "Paenibacillus" patented technology

The invention relates to the technical field of plant tissue culture seedling raising, in particular to paenibacillus feed and application thereof in promoting cunninghamia lanceolata test-tube plantlet height growth. The feed paenibacillus paenibacillus SY0317 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC NO: 20575, the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation time is August 31, 2020. The cunninghamia lanceolata test-tube plantlet cultured by adopting the strain has high growth speed, the growth amount is increased by more than 60%, the culture time of the cunninghamia lanceolata tissue culture plantlet is shortened, large-scale production of the cunninghamia lanceolata tissue culture plantlet can be realized, and the strain has remarkable economic and social benefits.

The invention provides a high-activity chitinase mutant and application thereof in degradation of crustacean waste, and belongs to the technical field of enzyme engineering and agricultural waste conversion. According to the invention, chitinase PbChi from Paenibacillus barengltzii is subjected to molecular modification, and a plurality of chitinase mutants with high activity are successfully constructed by increasing the flexibility of a connexin, changing steric hindrance at a substrate catalytic crack inlet and changing catalytic residues in a beta sheet layer in a catalytic structural domain through calculation design and experimental verification; the chitinase mutant can effectively degrade shrimp shell meal, so that carapace waste is effectively utilized, and the chitinase mutant has a wide application prospect in the biological feed industry.

The invention relates to the technical field of kitchen waste treatment, in particular to a solid fungicide, a preparation method and application. The solid microbial inoculum comprises a microbial inoculum and a carrier; the microbial agent is prepared from pseudoochrobactrum saccharum, alcaligenes faecalis, achromobacter xylosoxidans, paenibacillus amyloliquefaciens, bacillus cereus, sphingobium nicotianae, serratia marcescens, stenotrophomonas maltophilia, sphingobium humus, sphingobium aquaticum and bacillus subtilis; the carrier for carrying the liquid compound bacteria is bagasse, and the maximum number of released bacteria is 2.2 * 10 < 9 > CFU / g. When the solid microbial inoculum is added into a 18 L kitchen waste reactor, the waste decrement rate is 80% within 7 days, and the COD removal rate can reach 79.1% at most. According to the solid microbial agent disclosed by the invention, organic substances in the kitchen waste can be degraded while the kitchen waste is converted from a solid state to a liquid state, so that volume reduction and weight reduction of the kitchen waste are realized; and the solid microbial inoculum is convenient to transport and operate and low in cost, has positive significance on difficulties faced by kitchen waste treatment, and has a good application prospect.

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

The invention discloses a strain of paenibacillus volcano ZTUXWS1 derived from excrement of wild black neck crane and application of the strain, and belongs to the field of microorganisms, the strain is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M 20242603. According to the present invention, a culture medium is selected through cellulose, and the Paenibacillus cinereus ZTUXWS1 is obtained by carrying out strain separation on the part, not in contact with the environment, in the fresh faeces of wild birds by using the cellulose to provide the only carbon source, such that the Paenibacillus cinereus ZTUXWS1 can survive in the intestinal tract and can provide the related effect; meanwhile, a nematode toxicity sensitivity test proves that the compound has no obvious toxic effect on nematodes and can be used for degrading cellulose.

The invention discloses a growth-promoting, drought-resisting and silicon-decomposing complex microbial inoculant as well as a preparation method and application thereof, and belongs to the field of agricultural microbial preparations, the complex microbial inoculant is prepared from kluyvera medians L5N1, pseudomonas lini S2-1 and bacillus mucilaginosus JH3, the mass ratio of the L5N1 to the S2-1 to the JH3 is (1-3): (1-3): (1-5), and the mass ratio of the L5N1 to the S2-1 to the JH3 is (1-3): (1-5). The complex microbial inoculant can be loaded by adopting an organic fertilizer and / or attapulgite, and is used for promoting growth, resisting drought and decomposing silicon of the tobaccos; according to the invention, the silicon dissolving function, ACC deaminase production capacity, IAA production capacity, siderophore production capacity and phosphate solubilizing capacity of microorganisms are synergistically acted on plants, and the three microorganisms are used for jointly assisting the plants in resisting drought stress and promoting the growth of the plants.

Disclosed herein are compositions and methods for preventing, ameliorating, or treating pathogenic infections in aquaculture and / or reducing the severity of one or more risk factors, signs, or symptoms associated with pathogenic infections in aquaculture. In particular, the technology of the present disclosure relates to methods for administering an effective amount of a composition comprising a Paenibacillus strain, identified as PR-D9, to a subject suffering from or at risk for a pathogenic infection associated with aquaculture.

The invention relates to the technical field of plant disease prevention and control, in particular to a method for rapidly screening biocontrol bacteria of Candidatus Liberobacter asiaticum and application of the method. The rapid screening method comprises the following steps: mixing an Lcr-GFP bacterial suspension and a to-be-screened strain CFS according to a volume ratio of 1: 1-17: 3, incubating for more than 3 h, detecting fluorescence intensity when the excitation wavelength is 474-490 nm and the emission wavelength is 533-549 nm, and screening the biocontrol bacteria with the fluorescence quenching rate being greater than or equal to 10%. According to the present invention, the screened bacterial strain Paenibacillus edorifer has the inhibition effect on Lcr-GFP, and has the inhibition effect on Clas colonization in the in vivo detection of HLB infected plants, such that the Lcr-GFP inhibition effect can be provided, and the Lcr-GFP inhibition effect can be provided; therefore, the method for rapidly screening the biocontrol bacteria can effectively identify and screen the biocontrol bacteria with remarkable inhibitory activity on Clas, and has the biocontrol potential of HLB prevention and control.

The invention discloses an atrazine degrading strain and application thereof, the strain is a strain HB172176, the taxonomic name of the strain HB172176 is Paenibacillus Danchanensis, the preservation number of the strain HB172176 is CGMCC (China General Microbiological Culture Collection Center) NO.1. 16820, and the preservation date of the strain HB172176 is September 9, 2021. The invention further discloses application of the atrazine degrading strain in preparation of atrazine degrading products. The strain is separated from mangrove forest habitat with changeable environmental conditions, has atrazine degradation activity, has wide adaptability to pH and salinity, and can be applied in more environments.

This invention discloses a method, system, equipment, and medium for activating and promoting the release of humic acid from silkworm excrement. The method includes: mechanochemical synergistic crystal-breaking pretreatment, i.e., dry ball milling to destroy the physical encapsulation structure and induce a local alkaline microenvironment under chemical solvent-free conditions; stepwise enzymatic hydrolysis bridging activation, utilizing the acid buffering properties of silkworm excrement, sequentially adding uricase, cellulase / hemicellulase, and laccase or manganese peroxidase to form a natural pH fluctuation curve; directed microbial induction and release promotion, inoculating with Bacillus amyloliquefaciens and Bacillus lentigines for liquid fermentation to achieve metal ion chelation and surface charge regulation; and finally, low-temperature drying and molding to obtain the product. This invention also discloses the system, computer equipment, and storage medium for implementing this method. This invention does not require the addition of external acids, alkalis, or chemical chelating agents, shortens the total cycle to 2-4 days, and the obtained product has a high water-soluble humic acid content, which enhances the resistance to refixation after application to the soil.

This invention discloses an alkali-resistant xanthan gum side-chain lyase mutant and its preparation method, which is effective against alkali-resistant xanthan gum from Bacillus subtilis (Bacillus spp.). Paenibacillus nanensis Site-directed mutagenesis was performed on wild-type xanthan gum side-chain lyase to obtain alkali-resistant xanthan gum side-chain lyase mutants. The site-directed mutagenesis included at least one of Y260V, T387A, and I422S. The xanthan gum side-chain lyase mutants of this invention showed significantly increased enzyme activity compared to the original enzyme after treatment with an alkaline buffer system at 40°C, with the three mutants Y260V / T387A / I422S showing the greatest increase. These mutants are suitable for large-scale degradation of xanthan gum side chains under alkaline conditions, possessing significant social and economic benefits and broad application prospects.

The present application belongs to the technical field of tobacco planting, and provides a whole-cycle planting method for improving quality and increasing yield of tobacco, comprising the following steps: (1) seed treatment: after cleaning the seeds with water, the seeds are soaked in an elicitor composed of gibberellin with a concentration of 50-1000 mg / L and 10-15 mL / L of Bacillus polymyxa dgnkzx004 fungicide fermentation liquor, then subjected to light treatment, and washed and dried; (2) seedling raising: mixed bacteria cow manure fermentation products are added to the seedling raising substrate, and Bacillus polymyxa dgnkzx004 fermentation liquor is applied for seedling raising; (3) transplanting: the tobacco seedlings are transplanted, and the mixed bacteria cow manure fermentation products are used as fertilizer for fertilization. The present application promotes seed germination rate, improves seedling emergence rate and seedling survival rate, and can significantly improve the growth potential of tobacco root system and the yield of tobacco, and significantly reduce the arsenic content in tobacco leaves.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a bacillus PY4 strain capable of efficiently antagonizing various phytopathogens and application of the bacillus PY4 strain, and belongs to the technical field of biological prevention and control of plant diseases. The strain has stable morphological characteristics, and is identified according to a 16S rRNA (ribosomal Ribonucleic Acid) sequence, and the strain is a Paenibacillus bacterium. An in-vitro confrontation test proves that the PY4 can effectively inhibit pathogenic fungi such as sclerotinia sclerotiorum, fusarium graminearum, pseudocauda sp, alternaria alternata and sclerotium rolfsii. Field application results show that the PY4 has a good prevention and treatment effect on sclerotinia rot of colza. The invention further provides a biocontrol agent based on the strain and an application method of the biocontrol agent. The biocontrol agent can be used for green prevention and control of soil-borne and leaf-borne fungal diseases of crops.

This invention discloses a strain of *Bacillus paramycoides* and its application in selenium oxidation, belonging to the field of agricultural microbiology. A strain of *Bacillus paramycoides* H1 was isolated from the soil of a selenium-nutrition experimental field at Huazhong Agricultural University and deposited at the China Center for Type Culture Collection (CCTCC) on June 27, 2023, with accession number CCTCCNO: M 20231107. Experimental verification showed that this strain can simultaneously oxidize elemental selenium and organic selenium. This invention utilizes this strain to convert organic and elemental selenium into water-soluble selenium that promotes plant absorption, laying a scientific foundation for the preparation of safe and low-cost plant selenium-enriching agents and selenium oxidants. This is of great significance for the research and agricultural cultivation of selenium conversion model strains.

This invention belongs to the field of bio-fermentation technology and relates to a strain of Bacillus videmannii SXZY-01 and its applications, including: isolating and screening a strain of Bacillus videmannii capable of producing tanninase from the surface of tobacco. Bacillus wiedmannii The strain SXZY-01, with accession number CCTCC NO:M 2025151, was used to ferment a biological agent containing SXZY-01. After fermentation optimization, the tannin enzyme activity reached 40.98 U / mL. This invention applies a high-enzyme-activity solution to tobacco leaf fermentation and optimizes the fermentation process, achieving efficient degradation of tannins, significantly improving the sensory quality of tobacco, reducing the bitter aftertaste, and demonstrating outstanding technical effects. It provides a green and environmentally friendly solution for upgrading tobacco quality and is in line with the industry's high-quality development direction.

The invention discloses an alkali-resistant xanthan gum side chain lyase mutant and a preparation method thereof, the alkali-resistant xanthan gum side chain lyase mutant is obtained by carrying out site-specific mutagenesis on wild type xanthan gum side chain lyase from paenibacillus nanensis, and the mutation site of the site-specific mutagenesis comprises at least one of Y260V, T387A and I422S. According to the xanthan gum side chain lyase mutant disclosed by the invention, the enzyme activity of the mutant treated by using an alkaline buffer system is obviously improved compared with that of an original enzyme at 40 DEG C, and the enzyme activity is improved to the greatest extent by using the three mutants Y260V / T387A / I422S, so that the xanthan gum side chain lyase mutant is suitable for large-scale degradation of a xanthan gum side chain under an alkaline condition, has important social and economic benefits and is wide in application prospect.

The invention relates to the field of vegetable planting, in particular to a method for applying phosphate solubilizing bacteria extracted from tea garden soil to leaf lettuce growth promotion. The invention relates to a method for applying phosphate solubilizing bacteria extracted from tea garden soil to growth promotion of leaf lettuce, which comprises the following steps: selecting an activated strain, placing the strain in a triangular flask filled with an LB liquid culture medium, culturing on a reciprocating shaking table until bacterial liquid is slightly turbid, measuring the absorbance value of bacterial suspension at the wavelength of 600nm by using a spectrophotometer, measuring the OD600 value of the bacterial suspension to 0.7-0.9, and calculating the content of the phosphate solubilizing bacteria. A pipette is used for sucking bacterial liquid, 0.8% normal saline is added for constant volume, and then lettuce root irrigation treatment is carried out; the bacterial strain is one of bacillus velezensis, bacillus thuringensis, Burkholderia, Burkholderia, and Paenibacillus lentigera, and the bacterial strain is one of bacillus velezensis, bacillus thuringensis, Burkholderia, Burkholderia, and Paenibacillus lentigera.

The application discloses a Paenibacillus sp. NGMCC 1.200843 derived from rhinoceros feces, belongs to a new discovery in the technical field of microorganisms, and is identified as a new species of Paenibacillus, numbered as NGMCC 1.200843=CGMCC 1.64763T, and has been registered and preserved in the China General Microbiological Culture Collection Center. The strain can form a larger transparent circle on a specific culture medium, indicating that the strain has strong phosphorus and starch degradation capacity. Under the current situation of soil fertility decline and fertilizer abuse, the strain provides a high-quality strain resource for the development of microbial fertilizers with organic phosphorus degradation function, and helps to promote the development of microbial fertilizers. The hydrolytic enzymes produced by the strain have wide application potential in the industrial fields of food, fermentation and pharmaceuticals. In particular, amylase can hydrolyze starch to produce various products, and has important significance for the biotechnology industry, and the application range covers multiple industries such as food, fermentation, textile and papermaking. In a word, the application provides a new resource for the development of microbial fertilizers and industrial enzyme preparations.

This invention relates to the field of agricultural microbiology, and in particular to a strain of *Bacillus erythropoietinus* KF-B6 and its applications, especially in the control of continuous cropping obstacles in apple orchards. The *Bacillus erythropoietinus* KF-B6, with preservation number CGMCC No. 32131, possesses a complex set of functions including rapid and high EPS (explosive peroxide) production, high chitinase production, production of water-soluble and volatile antibacterial substances, and decomposition of macromolecular organic matter. The application of this bacterium, its microbial agents, and compound microbial fertilizers can effectively increase the number of rhizosphere soil bacteria, reduce the number of pathogenic fungi, increase the ratio of bacteria to fungi, and promote the recovery of the soil microbial ecosystem in apple orchards with continuous cropping obstacles; increase the proportion of water-stable aggregates, increase soil organic matter content, and increase soil enzyme activity, thus improving the soil; improve the survival rate of saplings planted during the renewal of new varieties in old orchards with continuous cropping obstacles, promote root vitality and plant growth, and comprehensively alleviate and control continuous cropping obstacles in apple orchards.

The invention belongs to the technical field of microorganisms, discloses a paenibacillus zeae strain and application of the paenibacillus zeae strain in prevention and treatment of plant diseases, and particularly relates to application of the paenibacillus zeae strain in prevention and treatment of lycium barbarum root rot. The paenibacillus zeae is separated from rhizosphere soil of a Chinese wolfberry plant suffering from root rot, the strain number is R2, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36137. The invention further discloses a preparation method of the paenibacillus zeae. The strain can inhibit the growth of various fungal pathogenic bacteria including lycium barbarum root rot, can generate cellulase and amylase, and can induce the expression of defensive genes such as PR1 and CAT2 of plants. Pot experiments show that the control effect of the strain on lycium barbarum root rot reaches 66.66%, the disease morbidity and disease index can be remarkably reduced, and effective biological resources are provided for green control of plant diseases, especially the lycium barbarum root rot.

The application belongs to the technical field of bioactive substance preparation and agricultural application, and discloses a beta-amino butyric acid, a whole cell conversion preparation method thereof and application thereof. The method uses Paenibacillus halophilus as a catalyst to perform biological conversion in a reaction system containing a substrate, so as to obtain the beta-amino butyric acid. The method has mild reaction conditions, the bacterial cells can be repeatedly used, the conversion rate is high, and the green, efficient and low-cost production of the beta-amino butyric acid is realized. The application also provides application of the beta-amino butyric acid prepared by the above method in improving salt stress resistance of crops and in plant disease control as a pesticide synergist. Experiments show that the beta-amino butyric acid can significantly alleviate the inhibition of salt stress on the growth of wheat, and improve the chlorophyll content and biomass accumulation; the beta-amino butyric acid has a synergistic effect on grape diseases when used in combination with anilazodifen and fluoroacetamide, and meanwhile, the fruit quality and storage resistance are improved.

The application belongs to the field of application of agricultural microorganism and biological control technology, and particularly relates to a bacillus chagamensis SY7-28, a microbial agent, a microbial pesticide and application. Fictibacillus barbaricus The bacillus chagamensis (Bacillus chagamensis) SY7-28 is preserved in the China General Microbiological Culture Collection Center on June 27, 2025, and the strain preservation number is CGMCC No. 35029. The bacillus chagamensis SY7-28, the microbial agent and the microbial pesticide disclosed by the application not only have a control effect on tomato bacterial wilt, but also have a significant growth promotion effect.

The application discloses a kind of organic fertilizer based on biomass fermentation and its preparation method and application, belong to biological organic fertilizer technical field.The organic fertilizer based on biomass fermentation of the application includes the following raw materials: chicken manure 40-50 parts, rapeseed cake 20-30 parts, seaweed powder 15-20 parts, corn straw 10-15 parts, EM bacterial liquid 10-15 parts, compound microbial inoculant 6-10 parts, Rhizoma Cyperi 8-16 parts, mulberry branch 4-8 parts, water hyacinth 12-18 parts, mushroom residue 5-8 parts, wherein the compound microbial inoculant is composed of jelly-like bacillus, sand on flavobacterium, rhizosphere oligotrophic single-cell bacteria, which can quickly decompose organic matter, accelerate the production of small molecules, and be directly absorbed by plants and produce a variety of active substances during fermentation.The organic fertilizer of the application has a synergistic effect of multiple substances, significantly promotes photosynthesis of plants, improves nutrient production and transportation, effectively promotes growth of Chinese cabbage and improves quality of Chinese cabbage.

This invention discloses a multifunctional Paenibacillus polymyxa with straw-degrading, broad-spectrum antibacterial, and highly effective insecticidal activities, and its applications, belonging to the field of agricultural microbiology. The strain provided by this invention is Paenibacillus polymyxa BMB-Pp0021, with the strain preservation number CCTCC NO.M20241951. This strain not only exhibits broad-spectrum antibacterial activity but also highly effective insecticidal properties, and simultaneously possesses the ability to efficiently degrade straw, especially under low-temperature conditions (7-day weight loss rate of 50.20%). These multifunctional characteristics give it enormous application potential in agriculture and industry.

The invention aims to provide a novel polymyxin compound as well as a preparation method and application thereof. The compound provided by the invention is shown as a formula (IV), r1 is (CH3) 2CH or CH3CH2CH (CH3) or CH3CH2CH2, and R2 is CH3CH2CH3 or CH3CH2CH2; and R2 is H or CH. The invention also protects the application of the compound or the pharmaceutically acceptable salt thereof in preparation of a bacterial inhibitor. The invention further provides the paenibacillus polymyxa CPCC 101041 and the application of the paenibacillus polymyxa. The compound is obtained by being separated from a fermentation product of paenibacillus polymyxa CPCC 101041. The invention further discloses a preparation method of the compound. # imgabs0 #

The application provides a strain of mineralized soil organic phosphorus, Domibacillus indicus D99, and application of the strain in soil organic phosphorus mineralization, growth promotion of phosphorus solubilizing bacteria and plant growth promotion. The application first shows the function and role of Domibacillus indicus in soil organic phosphorus mineralization and plant growth promotion, and has important value for development of phosphorus solubilizing bacteria fertilizer and other microbial fertilizers. The strain of Domibacillus indicus D99 provided by the application has the ability of dissolving soil organic phosphorus, the acid phosphatase activity is 17.32, the alkaline phosphatase activity is 1.3, can promote plant growth, and improves the aboveground biomass by 50-115.

The invention discloses a synthetic flora, a complex microbial inoculant thereof and application of the complex microbial inoculant in bacterial wilt prevention and treatment, and aims to solve the technical problems that the current bacterial wilt prevention and treatment effect is poor and the resistance of plants to ralstonia solanacearum is low. According to the application, the Rohe bacillus and the paenibacillus separated from a plant root system subjected to high-calcium treatment and the pseudomonas aeruginosa EZ-35 separated in the early stage can antagonize the ralstonia solanacearum and have different degrees of preference ability to calcium nutrition, and the three strains are matched and combined into a synthetic flora, so that the resistance of the plant to the ralstonia solanacearum can be enhanced; furthermore, the synthetic flora is combined with a calcium fertilizer to prevent and treat bacterial wilt, so that the morbidity can be remarkably reduced.