43 results about "Paenibacillus" patented technology

The invention belongs to the technical field of food processing, and relates to a method for preparing low-salt shrimp juice through rapid microbial fermentation. The method comprises the following steps: removing heads of shrimps, preparing meat paste, drying and crushing shrimp shells, and mixing the shrimp shells with the meat paste; carrying out enzymolysis treatment on the meat paste by using alkaline protease, and then carrying out high-temperature enzyme deactivation; mixing the shrimp meat and shrimp shell enzymatic hydrolysate mixture with soybean meal and bran, and sterilizing at high temperature; inoculating a complex microbial inoculant containing lactobacillus plantarum, saccharomyces rouxii, aspergillus oryzae, deep-sea bacillus subtilis and bacillus subtilis to prepare a mold starter, fermenting at 28 DEG C for 30 days, and finally filtering and sterilizing. According to the method disclosed by the invention, the availability of the raw materials is effectively improved, the fermentation period is shortened, efficient fermentation under a low-salt condition is realized, various basic indexes of the obtained shrimp juice product meet national standards, and the shrimp juice product is outstanding in flavor quality and has rich sauce flavor and sweet taste. In addition, the shrimp juice shows good antioxidant stability before and after digestion.

This invention discloses a method, system, equipment, and medium for activating and promoting the release of humic acid from silkworm excrement. The method includes: mechanochemical synergistic crystal-breaking pretreatment, i.e., dry ball milling to destroy the physical encapsulation structure and induce a local alkaline microenvironment under chemical solvent-free conditions; stepwise enzymatic hydrolysis bridging activation, utilizing the acid buffering properties of silkworm excrement, sequentially adding uricase, cellulase / hemicellulase, and laccase or manganese peroxidase to form a natural pH fluctuation curve; directed microbial induction and release promotion, inoculating with Bacillus amyloliquefaciens and Bacillus lentigines for liquid fermentation to achieve metal ion chelation and surface charge regulation; and finally, low-temperature drying and molding to obtain the product. This invention also discloses the system, computer equipment, and storage medium for implementing this method. This invention does not require the addition of external acids, alkalis, or chemical chelating agents, shortens the total cycle to 2-4 days, and the obtained product has a high water-soluble humic acid content, which enhances the resistance to refixation after application to the soil.

This invention discloses an alkali-resistant xanthan gum side-chain lyase mutant and its preparation method, which is effective against alkali-resistant xanthan gum from Bacillus subtilis (Bacillus spp.). Paenibacillus nanensis Site-directed mutagenesis was performed on wild-type xanthan gum side-chain lyase to obtain alkali-resistant xanthan gum side-chain lyase mutants. The site-directed mutagenesis included at least one of Y260V, T387A, and I422S. The xanthan gum side-chain lyase mutants of this invention showed significantly increased enzyme activity compared to the original enzyme after treatment with an alkaline buffer system at 40°C, with the three mutants Y260V / T387A / I422S showing the greatest increase. These mutants are suitable for large-scale degradation of xanthan gum side chains under alkaline conditions, possessing significant social and economic benefits and broad application prospects.

The present application belongs to the technical field of tobacco planting, and provides a whole-cycle planting method for improving quality and increasing yield of tobacco, comprising the following steps: (1) seed treatment: after cleaning the seeds with water, the seeds are soaked in an elicitor composed of gibberellin with a concentration of 50-1000 mg / L and 10-15 mL / L of Bacillus polymyxa dgnkzx004 fungicide fermentation liquor, then subjected to light treatment, and washed and dried; (2) seedling raising: mixed bacteria cow manure fermentation products are added to the seedling raising substrate, and Bacillus polymyxa dgnkzx004 fermentation liquor is applied for seedling raising; (3) transplanting: the tobacco seedlings are transplanted, and the mixed bacteria cow manure fermentation products are used as fertilizer for fertilization. The present application promotes seed germination rate, improves seedling emergence rate and seedling survival rate, and can significantly improve the growth potential of tobacco root system and the yield of tobacco, and significantly reduce the arsenic content in tobacco leaves.

The invention relates to the technical field of microorganisms, and particularly provides application of bacillus velezensis Y1062 in crop growth promotion and verticillium dahliae antagonism. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.36497, has various probiotic functions of dissolving phosphorus and potassium, secreting cellulase, amylase and protease and the like, and can tolerate high temperature and ultraviolet stress. Meanwhile, the strain has a broad-spectrum antagonistic effect on various phytopathogens such as verticillium dahliae, fusarium oxysporum and the like. The pot experiment further proves that the disease index of the cotton verticillium wilt and the biomass of pathogenic bacteria at the root can be remarkably reduced by inoculating the strain, the growth promoting effect is remarkable, and the root development and plant growth of crops can be remarkably promoted by applying the strain to the crops. The invention provides a special'growth-promoting and disease-resisting 'functional strain resource for crops such as cotton and the like, and has important significance.

The invention discloses a bacillus PY4 strain capable of efficiently antagonizing various phytopathogens and application of the bacillus PY4 strain, and belongs to the technical field of biological prevention and control of plant diseases. The strain has stable morphological characteristics, and is identified according to a 16S rRNA (ribosomal Ribonucleic Acid) sequence, and the strain is a Paenibacillus bacterium. An in-vitro confrontation test proves that the PY4 can effectively inhibit pathogenic fungi such as sclerotinia sclerotiorum, fusarium graminearum, pseudocauda sp, alternaria alternata and sclerotium rolfsii. Field application results show that the PY4 has a good prevention and treatment effect on sclerotinia rot of colza. The invention further provides a biocontrol agent based on the strain and an application method of the biocontrol agent. The biocontrol agent can be used for green prevention and control of soil-borne and leaf-borne fungal diseases of crops.

This invention discloses a strain of *Bacillus paramycoides* and its application in selenium oxidation, belonging to the field of agricultural microbiology. A strain of *Bacillus paramycoides* H1 was isolated from the soil of a selenium-nutrition experimental field at Huazhong Agricultural University and deposited at the China Center for Type Culture Collection (CCTCC) on June 27, 2023, with accession number CCTCCNO: M 20231107. Experimental verification showed that this strain can simultaneously oxidize elemental selenium and organic selenium. This invention utilizes this strain to convert organic and elemental selenium into water-soluble selenium that promotes plant absorption, laying a scientific foundation for the preparation of safe and low-cost plant selenium-enriching agents and selenium oxidants. This is of great significance for the research and agricultural cultivation of selenium conversion model strains.

This invention belongs to the field of bio-fermentation technology and relates to a strain of Bacillus videmannii SXZY-01 and its applications, including: isolating and screening a strain of Bacillus videmannii capable of producing tanninase from the surface of tobacco. Bacillus wiedmannii The strain SXZY-01, with accession number CCTCC NO:M 2025151, was used to ferment a biological agent containing SXZY-01. After fermentation optimization, the tannin enzyme activity reached 40.98 U / mL. This invention applies a high-enzyme-activity solution to tobacco leaf fermentation and optimizes the fermentation process, achieving efficient degradation of tannins, significantly improving the sensory quality of tobacco, reducing the bitter aftertaste, and demonstrating outstanding technical effects. It provides a green and environmentally friendly solution for upgrading tobacco quality and is in line with the industry's high-quality development direction.

The invention discloses an alkali-resistant xanthan gum side chain lyase mutant and a preparation method thereof, the alkali-resistant xanthan gum side chain lyase mutant is obtained by carrying out site-specific mutagenesis on wild type xanthan gum side chain lyase from paenibacillus nanensis, and the mutation site of the site-specific mutagenesis comprises at least one of Y260V, T387A and I422S. According to the xanthan gum side chain lyase mutant disclosed by the invention, the enzyme activity of the mutant treated by using an alkaline buffer system is obviously improved compared with that of an original enzyme at 40 DEG C, and the enzyme activity is improved to the greatest extent by using the three mutants Y260V / T387A / I422S, so that the xanthan gum side chain lyase mutant is suitable for large-scale degradation of a xanthan gum side chain under an alkaline condition, has important social and economic benefits and is wide in application prospect.

The invention belongs to the technical field of microorganisms, discloses a paenibacillus zeae strain and application of the paenibacillus zeae strain in prevention and treatment of plant diseases, and particularly relates to application of the paenibacillus zeae strain in prevention and treatment of lycium barbarum root rot. The paenibacillus zeae is separated from rhizosphere soil of a Chinese wolfberry plant suffering from root rot, the strain number is R2, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.36137. The invention further discloses a preparation method of the paenibacillus zeae. The strain can inhibit the growth of various fungal pathogenic bacteria including lycium barbarum root rot, can generate cellulase and amylase, and can induce the expression of defensive genes such as PR1 and CAT2 of plants. Pot experiments show that the control effect of the strain on lycium barbarum root rot reaches 66.66%, the disease morbidity and disease index can be remarkably reduced, and effective biological resources are provided for green control of plant diseases, especially the lycium barbarum root rot.

The application belongs to the technical field of bioactive substance preparation and agricultural application, and discloses a beta-amino butyric acid, a whole cell conversion preparation method thereof and application thereof. The method uses Paenibacillus halophilus as a catalyst to perform biological conversion in a reaction system containing a substrate, so as to obtain the beta-amino butyric acid. The method has mild reaction conditions, the bacterial cells can be repeatedly used, the conversion rate is high, and the green, efficient and low-cost production of the beta-amino butyric acid is realized. The application also provides application of the beta-amino butyric acid prepared by the above method in improving salt stress resistance of crops and in plant disease control as a pesticide synergist. Experiments show that the beta-amino butyric acid can significantly alleviate the inhibition of salt stress on the growth of wheat, and improve the chlorophyll content and biomass accumulation; the beta-amino butyric acid has a synergistic effect on grape diseases when used in combination with anilazodifen and fluoroacetamide, and meanwhile, the fruit quality and storage resistance are improved.

The application discloses a kind of organic fertilizer based on biomass fermentation and its preparation method and application, belong to biological organic fertilizer technical field.The organic fertilizer based on biomass fermentation of the application includes the following raw materials: chicken manure 40-50 parts, rapeseed cake 20-30 parts, seaweed powder 15-20 parts, corn straw 10-15 parts, EM bacterial liquid 10-15 parts, compound microbial inoculant 6-10 parts, Rhizoma Cyperi 8-16 parts, mulberry branch 4-8 parts, water hyacinth 12-18 parts, mushroom residue 5-8 parts, wherein the compound microbial inoculant is composed of jelly-like bacillus, sand on flavobacterium, rhizosphere oligotrophic single-cell bacteria, which can quickly decompose organic matter, accelerate the production of small molecules, and be directly absorbed by plants and produce a variety of active substances during fermentation.The organic fertilizer of the application has a synergistic effect of multiple substances, significantly promotes photosynthesis of plants, improves nutrient production and transportation, effectively promotes growth of Chinese cabbage and improves quality of Chinese cabbage.

This invention discloses a multifunctional Paenibacillus polymyxa with straw-degrading, broad-spectrum antibacterial, and highly effective insecticidal activities, and its applications, belonging to the field of agricultural microbiology. The strain provided by this invention is Paenibacillus polymyxa BMB-Pp0021, with the strain preservation number CCTCC NO.M20241951. This strain not only exhibits broad-spectrum antibacterial activity but also highly effective insecticidal properties, and simultaneously possesses the ability to efficiently degrade straw, especially under low-temperature conditions (7-day weight loss rate of 50.20%). These multifunctional characteristics give it enormous application potential in agriculture and industry.

The invention provides a saline-alkali soil conditioner and a preparation method thereof. The preparation method comprises the following steps: S1, preparing polystyrene microspheres coated with polyethyleneimine; s2, preparing attapulgite of which the surface is grafted with TEMPO oxidized cellulose, and then carbonizing the attapulgite to obtain a carbon-coated attapulgite composite material; s3, preparing composite aerogel; s4, bacillus pasteurii and azotobacter chroococcum are loaded on the composite aerogel, and the saline-alkali soil conditioner is obtained. The double strains are loaded on the composite aerogel, controlled release and slow release of the strains are achieved, in addition, the two microbial agents serve as dominant flora, breeding of harmful pathogens in soil can be inhibited, soil micro-ecological balance can be adjusted, and a healthy rhizosphere environment is created for follow-up crop planting.

The application discloses a bacillus mycoides BMS-39 and application thereof, and belongs to the technical field of microorganisms and agricultural biological control. Fulvia fulva The strain BMS-39 has a very strong inhibiting effect, the inhibiting rate reaches 92.3% by means of a flat-plate confrontation method, the inhibiting rate of 10% fermentation filtrate reaches 87.6%, the potting prevention effect reaches 87.01% in 14 days, and the field prevention effect reaches 87.81%. Even when the concentration of the bacterial liquid is reduced to 10 6 CFU / mL, the prevention effect of the BMS-39 can still reach 81.46%, which means that the fermentation cost and application cost of the application can be greatly reduced under the premise of meeting the same or even better prevention effect, and the application has significant market competitiveness.

The invention provides bacillus mojavensis and application thereof. The bacillus mojavensis is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO. 35674. The bacillus mojavensis has the advantages that the bacillus mojavensis can be used as a bacillus mojavensis strain; the bacillus mojavensis has multiple biological activities: on one hand, phosphorus-solubilizing related metabolites, potassium-solubilizing related metabolites, siderophores and indoleacetic acid can be generated, and meanwhile, the bacillus mojavensis has the activity of 1-aminocyclopropane-1-carboxylic acid deaminase, so that the growth of seedlings of crops such as jujubes, cucumbers and the like can be remarkably promoted; and on the other hand, the strain has broad-spectrum bacteriostatic activity on 16 pathogenic fungi such as jujube fruit black skin pathogen and cucumber root rot pathogen, has excellent prevention and control effects on jujube black skin disease and cucumber root rot, and can effectively regulate and control a cucumber rhizosphere micro-ecological structure and improve crop disease resistance. Besides, the JZBQ1 strain also has remarkable salt-tolerant and drought-resistant characteristics, can stably play the roles of disease prevention, growth promotion and yield increase under complex cultivation conditions such as conventional cultivated land, saline-alkali soil and drought, and has huge application potential. The invention provides a new strain resource for development and application of an efficient biocontrol inoculant, and has important significance for promoting green development of fruits and vegetables and reducing dependence on chemical pesticides.

The invention discloses a functional microbial agent for near-natural recovery of moderately-degraded high-cold grassland as well as a preparation method and application of the functional microbial agent. The composite bacterial agent is prepared by mixing bacterial culture solutions of four bacterial strains, namely bacillus pumilus E-B-2-B05 (CGMCC No. 21387), paenibacillus silage P-B-2-B03 (CGMCC No. 21386), bacillus subtilis P-B-4-SCB23 (CGMCC No. 27084) and bacillus velezensis K-B-5-SCB13 (CGMCC No. 27088) according to the volume ratio of 3: 2.5: 2: 2.5. The invention further discloses a preparation method of the composite bacterial agent. Through the synergistic effect of multiple strains, the complex microbial inoculant can effectively promote plant growth, enhance rhizosphere microbial activity and improve the ecological process of soil, can effectively improve grassland biomass, optimize a plant community structure, enhance competitive advantages of high-quality pasture, and promote moderately-degraded alpine grassland vegetation to recover to a near-natural state. The microbial agent is simple in preparation method, convenient and flexible to apply and environment-friendly, and has good ecological and economic benefits.

The present invention addresses the problem of providing a microbial material that can also be applied to agricultural crop cultivation in a dry area.　The present invention provides the following endospore-forming bacteria (A) to (C) having plant-growth promoting activity in a dry environment. (A) Either Bacillus thuringiensis TTREp1 strain identified by the accession number NITE BP-04159 or Bacillus altitudinis TCo-SSREp1 strain identified by the accession number NITE BP-04160, or a mutant strain thereof (B) Paenibacillus polysaccharolyticus TP-WSREp1 strain identified by the accession number NITE BP-04163, or a mutant strain thereof (C) either Priestia aryabhattai TTREn1 strain identified by the accession number NITE BP-04165 or Priestia megaterium TC-WSREn1 strain identified by the accession number NITE BP-04166, or a mutant strain thereof

Paenibacillus bacterial strains are engineered to express or overexpress (i) one or more proteins (pyruvate ferredoxin oxidoreductase, flavodoxin, and / or ferredoxin) involved in the electron transport chain, (ii) the DUF protein and / or (iii) gene products of the suf operon. This enables a greater rate / level of nitrogen fixation by the diazotrophic bacteria. The engineered bacteria are introduced to agricultural plants, or seeds for growing such, forming a symbiotic relationship that results in increased nitrogen availability to the plant and improved plant agronomic traits.

The invention discloses a synthetic bacterial agent for inhibiting rhizosphere colonization of polymyxa cerealis serving as a wheat soil-borne virus transmission medium and application of the synthetic bacterial agent, and belongs to the technical field of environmental microorganism and plant disease prevention and control. The invention relates to a synthetic bacterial agent, which is characterized in that the synthetic bacterial agent is prepared from one or more of the following components: Massilla kyonggiensis, Paenibacillus silage, Paraburkholderia caledonica, Bacillus aryabhattai, Mycobacterium goodii and Sphingomonas azotifgens, and the synthetic bacterial agent is prepared from one or more of the following components: Sphingomonas kyonggiensis, Paenibacillus silage, Paraburkholderia caledonica, Bacillus aryabhattai, Mycobacterium goodii and Sphingomonas azotifgens. The synthetic bacterial agent disclosed by the invention is applied to plants, can obviously inhibit rhizosphere / root colonization of a wheat soil-borne virus transmission medium polymyxa graminearum, is safe, green and environment-friendly, and provides technical support for a green prevention and control technology of wheat virus diseases transmitted by the polymyxa graminearum.

The invention belongs to the technical field of agricultural biology, and particularly discloses a functional seedling culture substrate for preventing and treating root-knot nematode and application of the functional seedling culture substrate. The functional seedling raising substrate is composed of a solid seedling raising substrate and a compound microbial agent uniformly dispersed in the solid seedling raising substrate. The compound microbial agent is prepared by carrying out mixed fermentation on bacillus velezensis RKN1111, bacillus laterosporus CDD-01 and paenibacillus mucilaginosus HX-02. The invention further discloses a preparation method of the compound microbial agent. When the functional seedling culture substrate is used for seedling culture, the colonization rate of the compound microbial agent in seedling rhizosphere is greatly improved, the inside and the outside of root lumps of cultured germchit are rich in active biocontrol bacteria during transplanting, the germchit can continuously play a high-efficiency and stable prevention and control role after transplanting, and the growth of the germchit is remarkably promoted. According to the method, the prevention and control opportunity of the root-knot nematode is greatly advanced from a field period to a controllable seedling raising period, active immunization is achieved, and the technical problems that colonization is difficult and the effect is unstable when a biocontrol microbial inoculum is applied to traditional root irrigation or fields are effectively solved.

A method for preparing a protein that degrades pentachloronitrobenzene pesticide residues and a method for regulating trace elements are disclosed. The strain used in this method is *Bacillus polymyxa*, deposited at the China General Microbiological Culture Collection Center (CGMCC) under accession number CGMCC No. 7250. The method involves extracting *Bacillus polymyxa* protein with 40% saturated ammonium sulfate, which can be used to degrade pentachloronitrobenzene pesticide residues. The trace element Fe2+ significantly enhances the pentachloronitrobenzene degradation activity of *Bacillus polymyxa*, exhibiting strong adaptability to different terrains and the ability to adjust its working mode in real time according to environmental needs. The *Bacillus polymyxa* pesticide residue-degrading protein prepared by this invention can significantly degrade pentachloronitrobenzene pesticide residues in ginseng. The 40% saturated ammonium sulfate extraction of *Bacillus polymyxa* protein has the effect of degrading pentachloronitrobenzene, and the trace element Fe2+ significantly enhances its ability to degrade pentachloronitrobenzene. 2+ It significantly enhances the degradation of pentachloronitrobenzene by Bacillus polymyxa.

The invention belongs to the technical field of organic fertilizers, and particularly relates to a preparation method of a fly ash-based soil improvement type slow-release organic fertilizer. The porosity and the specific surface area of the fly ash are remarkably improved by carrying out synergistic pretreatment of ball milling, acid pickling, plasma treatment and microwave treatment on the fly ash; pretreated fly ash and sodium alginate are subjected to cross-linking solidification to construct a porous gel particle system, a high-activity sporosarcina pasteurii bacterial solution and a chemical fertilizer are loaded into porous gel particles through a vacuum impregnation technology, and uniform and efficient immobilization of functional microorganisms and nutrients is achieved; a compact biomineral coating layer is constructed on the surface of a bacterial fertilizer compound and the inner walls of pores through a microorganism-induced mineralization process, so that a novel fertilizer integrating the functions of a slow-release fertilizer, a soil conditioner and an organic fertilizer is prepared; the fertilizer shows excellent synergistic effects in the aspects of slow release performance, soil pH improvement, lasting nutrient supply and crop growth promotion and quality improvement.

The invention discloses a cyanide-reducing bio-adsorbent as well as a preparation method and application thereof, and relates to the technical field of mining wastewater treatment. The cyanide-reducing bio-adsorbent comprises paenibacillus and an immobilized carrier, the paenibacillus is fixed on the immobilization carrier; the bacillus is preserved in China General Microbiological Culture Collection Center (CGMCC) on May 14, 2025, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No. 34545. The cyanide-reducing bio-adsorbent provided by the invention can be used for adsorbing and treating cyanide in mining wastewater, and has the advantages of high adsorption rate, stable structure, high adsorption capacity, low cost, recyclability and the like.

This invention relates to the field of biofertilizer technology, specifically disclosing a microalgae biofertilizer with soil remediation function and its preparation method. The preparation method includes: domesticating and culturing *Scenedesmus obliquus* liquid, *Cyanobacterium cyanobacteria* liquid, and *Monoflagellates* liquid with γ-aminobutyric acid-induced activation solution, and co-culturing them with *Bacillus polymyxa* and *Bacillus megaterium* to obtain a bioslurry; encapsulating the bioslurry with sodium alginate-carboxymethyl chitosan to obtain bioactive microspheres; impregnating corn straw with magnesium-iron salt precursors and pyrolyzing it under nitrogen to obtain modified biochar loaded with magnesium oxide and iron oxide, and compounding it with potassium humate to obtain a biochar suspension; and mixing the bioactive microspheres, biochar suspension, xanthan gum, zeolite, ammonium polyphosphate, potassium nitrate, zinc sulfate, potassium silicate, and deionized water evenly to obtain the microalgae biofertilizer with soil remediation function.

The application belongs to the technical field of microbial application, and specifically provides a cold-tolerant Paenibacillus polymyxa (Y5R4-9). Peribacillus frigoritolerans Under the condition of saline-alkali stress, inoculation of the strain Y5R4-9 can promote the growth of forage oats in saline-alkali soil by improving the leaf antioxidant enzyme activity and proline content, reducing the MDA content, improving the soil enzyme activity and soil organic matter, increasing the plant height of forage oats, and improving the root morphology, so as to efficiently relieve the stress of saline-alkali on the growth of forage oats.

The invention discloses a composite microbial agent and application thereof in promoting plant growth. The invention discloses a compound microbial agent, which is characterized in that the compound microbial agent comprises Paenibacillus gelatinolyticus PG04 and Lysinibacillus capsici LC43, the compound microbial agent is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation numbers of the compound microbial agent are respectively CCTCC M 2026304 and CCTCC M 2026305. The two strains have excellent salt tolerance. The two components complement each other in function and have synergistic interaction. Pot experiments show that wheat growth can be remarkably promoted by applying the complex microbial inoculant. The microbial agent disclosed by the invention is eco-friendly and controllable in cost, and has a wide application prospect in agricultural utilization.

The invention belongs to the technical field of microorganisms, and particularly relates to a kitchen waste complex microbial inoculant as well as a preparation method and application thereof. The kitchen waste complex microbial inoculant comprises bacteria and fungi. The bacteria comprise bacillus thuringiensis, bacillus vedmannii, paenibacillus arachidicola, bacillus, preristeria koreensis and preristeria megatherium; the fungi comprise black powder saccharomycetes and fusarium laminatum. According to the method disclosed by the invention, the problems of obstruction of a hydrophobic membrane formed by grease and difficult degradation of cellulose are specifically solved by utilizing the synergistic effect of bacteria and fungi, so that the biodegradability of organic dry matters in the kitchen garbage is remarkably improved, and real source reduction is realized. Meanwhile, the invention provides a solid microbial inoculum which is subjected to double optimization of a fermentation process and a carrier adsorption process, so that the solid microbial inoculum can still keep high activity and high stability for a long time in an actual engineering environment of continuous feeding and salt accumulation, and the requirement of long-term continuous operation of distributed kitchen garbage treatment equipment is effectively met.

Paenibacillus bacterial strains are engineered to express or overexpress (i) one or more proteins (pyruvate ferredoxin oxidoreductase, flavodoxin, and / or ferredoxin) involved in the electron transport chain, (ii) the DUF protein and / or (iii) gene products of the suf operon. This enables a greater rate / level of nitrogen fixation by the diazotrophic bacteria. The engineered bacteria are introduced to agricultural plants, or seeds for growing such, forming a symbiotic relationship that results in increased nitrogen availability to the plant and improved plant agronomic traits.