Microbial flora capable of efficiently degrading PBAT plastic mulching film

A technology of plastic mulch and bacterial colony, applied in the direction of microorganisms, methods based on microorganisms, methods using microorganisms, etc., can solve the problems of incomplete degradation and slow degradation rate, and restrict the development of PBAT plastic mulch

Inactive Publication Date: 2018-05-08
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the plastic mulch film with PBAT as the main component is a biodegradable mulch film, the incomplete degradation and slow degradation rate of PBAT mulch film severely restrict the develop...

Method used

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  • Microbial flora capable of efficiently degrading PBAT plastic mulching film
  • Microbial flora capable of efficiently degrading PBAT plastic mulching film
  • Microbial flora capable of efficiently degrading PBAT plastic mulching film

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Screening of efficient PBAT plastic mulch degrading flora

[0017] Source of soil samples: The soil environment samples used in this experiment were from the greenhouse of Shandong Shouguang Vegetable Base, Fujian Anxi Tea Garden, Xinjiang 81, 84 Construction Corps, Guangzhou Compost Factory, Indonesian Palm Garden and Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences. Soil samples. The soil sampling depth is 5-10cm, and the collected soil samples are stored in completely sterilized transparent sealed bags and sealed bottles, numbered, sealed, and stored in a 4°C refrigerator for later use.

[0018] Preliminary screening process: The soil samples are the above-mentioned soil samples collected from all over the country, and they are enriched and cultivated in the above-mentioned newly invented SX medium with PBAT plastic mulch as the only carbon source and energy source. The specific operation is as follows: Add 10.0g of soil samples ...

Embodiment 2

[0020] Embodiment 2: Domestication of efficient PBAT plastic mulch degrading flora

[0021] Subculture and acclimatization of degrading bacteria: After initial discovery (adding 10.0g soil sample) that the PBAT plastic mulch can be degraded into a paste, the degradable bacterial solution of the soil treatment group was used as the first generation, and 10mL of the bacterial solution was taken again according to the 10% of the inoculation was transferred to the SX medium containing 300mgPBAT mulch film with a size of about 3cm×3cm for the second, third, fourth... N generations, and the transfer was performed once in 28 days.

[0022] In the SX medium with PBAT plastic mulch as the only carbon source, a microbial flora SX that can degrade PBAT plastic mulch was screened from the soil samples of Xinjiang 84 regiment on December 22, 2016, and PBAT plastic mulch was found A clear disintegration phenomenon can be found in it. After culturing at 37 degrees Celsius for 28 days, it wa...

Embodiment 3

[0024] Example 3: Analysis of the community structure of highly efficient PBAT plastic mulch degrading bacteria

[0025] The degrading bacteria group SX screened from soil samples in the cotton field of Xinjiang 84th Construction Corps after continuous domestication. Utilize SX culture medium of the present invention, its composition and proportioning (g / L) are as follows: NH 4 NO 3 1.0~2.0, MgSO 4 ·7H 2 O 0.2~1.0, K 2 HPO 4 1.0~2.0, CaCl 2 2H 2 O 0.1~1.0, KCl 0.15~0.5 and 1.0~10.0mg / L FeSO 4 ·6H 2 O,ZnSO 4 ·7H 2 O and MnSO 4, the pH is 7.6-8.0; the amount of PBAT plastic film added is 300 mg per 100 ml of the above medium, and the PBAT plastic film is used as the only carbon source. The flora mainly uses PBAT plastic mulch as the only carbon source required for growth. After continuous domestication, a group of complex microbial flora for the degradation of PBAT plastic mulch has been obtained, which has excellent removal properties when degrading PBAT plastic m...

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Abstract

The invention provides a microbial flora capable of efficiently degrading a PBAT plastic mulching film. The microbial flora includes brevibacillus borstelesis, paenibacillus, bacillus subtilis, bacillus amyloliquefaciens, sulfur streptococcus and vibrio leeches. The microbial flora capable of efficiently degrading the PBAT plastic mulching film is found for the first time at home and abroad; in the prior art, the physical properties of the PBAT plastic film are researched, and the technology for screening and utilizing the degrading flora are not found. After the flora is acclimatized, on thebasis of weight loss method calculation, the degrading efficiency for the PBAT plastic film can reach 99% or above within 13 d, and after degradation, naked eyes cannot observe residues of the formedPBAT mulching film.

Description

technical field [0001] The invention belongs to the technical field of functional microbial flora screening, domestication and compounding, and in particular relates to a microbial flora that efficiently degrades PBAT plastic mulches. Background technique [0002] With the rapid development of social economy, plastics are widely used in all aspects of social life. While it facilitates people's production and life, it also causes serious "white pollution" because it is extremely difficult to decompose in the natural environment. The disposal of these waste plastics will also cause serious environmental damage and pollution. Therefore, in recent years, biodegradable plastics have been favored by people as a new type of environmentally friendly polymer material, but they still degrade slowly and cannot be completely degraded in nature. The seriousness of the problem restricts the marketization and scale of biodegradable plastics. [0003] Nowadays, the use of microorganisms to...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/36A62D3/02C12R1/125C12R1/07C12R1/06C12R1/01A62D101/28
CPCA62D3/02A62D2101/28C12N1/20C12N1/36
Inventor 黄志勇徐松王敬敬周婷婷
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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