60 results about "Industrial biotechnology" patented technology

The invention concerns with the molecular biology, molecular genetics and biotechnology and can be used in the gene-therapy in the medicine and the agriculture or in the industrial biotechnology for a gene-specific silencing of the disease-related genes or the genes interfering a buildup of a product, respectively. The approach is suggested for changing of genetic properties of an organism by RNA interference leading to gene-specific silencing of a selected gene by RNA molecules, that are complementary in a parallel orientation (pcRNA) to mRNA of the selected gene; pcRNA are synthesized in vivo or in vitro on the artificial DNA sequence possessing symmetrical nucleotide ordering (mirror inversion) in respect to the nucleotide sequence of the gene. The invention suggests the general approach for changing of genetic properties of an organism and is based on the biological properties of mirror inversions of nucleotide sequences that are realized in RNA interference and gene-specific silencing.

The invention discloses a Yarrowia lipolytica and applications thereof, and belongs to the field of industrial biotechnology. A strain obtained after separation is named as Yarrowia lipolytica BBE-17. The strain can produce erythritol through fermentation using glucose as a carbon source, a high conversion rate can be realized without fed batch or continuous fermentation, and the adjustment of the pH value is not needed in the whole fermentation process, so the technologic operation is simplified, and the strain has certain industrial application potential. The strain also can produce xylitol through fermentation using a D-xylose mother liquor as a carbon source, so the strain a new potential way for the preparation of polyol products from non-food raw materials through a biological technology.

The invention belongs to the field of food industrial biotechnology, and relates to a whey protein film and a preparation method thereof. The whey protein film is prepared by adding whey protein and glycerol into purified water to prepare film-forming liquid, heating the film-forming liquid in 45-50 DEG C water bath for 0.5-2h, cooling to room temperature, adding recombinant lipoxygenase and linoleic acid, ultrasonically degassing, pouring into a glass culture dish covered with a plastic film, reacting for 2-3h at room temperature (20-25 DEG C), drying at 50-55 DEG C for 36-48h, and uncovering the film. With the whey protein as a based material and the glycerol as a plasticizer, the whey protein film prepared by adding the recombinant anabaena lipoxygenase as a crosslinking agent is green, edible and good in tensile strength, transparency, water absorption and plasticity.

The present application belongs to the field of functional peptides and more particularly to the field of controlled protein aggregation. The invention discloses molecules of a peptide structure as defined in the claims and methods of using such molecules for therapeutic applications and for diagnostic uses, as well as in other applications such as in the agbio field and in industrial biotechnology. The molecules can be used for curing and/or stabilizing infections such as bacterial,fungal and viral diseases, but are also useful in non-infectious human and veterinary diseases. The molecules can also be used for the detection of protein biomarkers and for the prognosis and diagnosis of a variety of diseases.

Application of protease and lipase to manufacture of a mulberry silk product has the following characteristics: the invention employs purified efficient alkaline lipase and protease and is accompanied with mature chemical refining method, so that silkworm cocoon or thin skin black cocoon that can not be used in silk reeling is mixed with alkaline lipase and protease, cooperated with a proper amount of surfactant, insulated naturally for 11.5-12.5 h with a bath ratio of 1:10-20 and a pH of 10.5-11 at 48-52 DEG C until cooled, taken from the pot, flushed, refined and treated with soft processing to obtain a fine draft sheet. The invention can well solve problems of degumming and oil removal in a prior silk spinning combing technology, reduce chemical raw material amount for producing per ton of silkworm fine dry draft by 2/3, save 5000 yuan of chemical material, 100 ton of water and 5 ton of coal for producing per ton of silkworm fine dry draft, promote application and popularization of industrial biological technology to silk inserts and processing of functional product, advance effective utilization and conversion increment of silk leftover and fill blanks of high quality velvet product home and abroad.

The invention relates to a recombined microorganism glutamine transaminase gene of a bacillus and expression thereof, belonging to the field of food industrial biotechnology. After the glutamine transaminase gene from the bacillus is cloned and separated, a lactococcus lactis recombinant expression vector spNZ8048-Tgase containing the gene is established and expressed in a lactococcus lactis N9800 strain; the recombined lactococcus lactis is named L.lactis; after an expression product is separated and purified, a recombined glutamine transaminase with a purity of about 90% is obtained; the activity of the recombined glutamine transaminase reaches 2.2U/mL which is greater than twice the activity of the original strain. Due to the adoption of the lactococcus lactis in production of the glutamine transaminase, the production method of the recombined microorganism glutamine transaminase gene has the advantages of high enzyme-producing activity, low production cost and good safety and is simple to operate, and the expressed enzyme is easy to separate and purify, so that a feasible manner for production of the glutamine transaminase is provided.

The invention discloses a product-resistant inhibitory mutated cephalosporin C acylase, a gene carrier and a transformant of the enzyme, and application of the enzyme in one-step enzymatic production of 7-aminocephalosporanic acid, belonging to the technical field of enzyme engineering and biotechnology industry. The amino acid sequence of the enzyme is obtained by conducting deletion mutation on the amino acid sequence of cephalosporin C acylase coded by the gene sequence SEQ ID NO:1 to obtain enzyme CPCAcy-D2 and CPCAcy-D4. The amino acid sequences are shown as SEQ ID NO:2 and SEQ ID NO:3, respectively. The invention also discloses the gene carrier, the transformant, and the application of the enzyme. The enzyme has high expressing activity, and also the tolerance of the product is improved significantly, so that CPC is catalyzed efficiently to produce 7-ACA.

The invention especially relates to a pretreatment method for biomass waste, which belongs to the field of industrial biotechnology. According to the method, water, divalent iron ions and a hydrogen dioxide solution are successively added into crushed biomass waste, the above-mentioned materials are uniformly mixed under stirring, and treatment is carried out for at least 5 min, so a pretreatment fluid of the biomass waste is obtained; the molar weight of the divalent iron ions to be added into each g of the biomass waste is 2.63 to 52.6 mM; and the molar weight of hydrogen peroxide to be added into each g of the biomass waste is 3.5 to 7.1 mM. The pretreatment method for the biomass waste is simple in process, mild in conditions, friendly to the environment and energy-saving; and the pretreatment method is a novel pretreatment mode corresponding to a biomass waste structure with cassava vinasse as a representative and provides a novel approach for low-cost and high-efficiency conversion of biomass waste with cassava vinasse as a representative.

The invention discloses heat-resistant Yarrowia Lipolytica and application thereof and belongs to the technical field of industrial biology. The heat-resistant Yarrowia Lipolytica is preserved in China Center for Type Culture Collection which is located in Wuhan University, Wuhan, China on March 18th, 2019, and the preservation number of the heat-resistant Yarrowia Lipolytica is CCTCC No. M2019163. The heat-resistant Yarrowia Lipolytica BBE-18 can ferment at 35 DEG C to produce erythritol, the erythritol yield of the heat-resistant Yarrowia Lipolytica BBE-18 reaches 68.1g/L when the fermentation time is 80 hours, and the erythritol conversion rate of glucose is 67%. The heat-resistant Yarrowia Lipolytica has certain industrialization potential and provides possibility for fermentation cooling cost lowering and summer production halt avoiding.

The invention discloses a method for improving biomass of lactic acid bacteria under the high salt condition and belongs to the technical field of bioengineering. Proline improves biomass of lactic acid bacteria in a high salt fermentation medium. The method has simple processes, is convenient for operation, effectively improves growth performances of lactic acid bacteria in a high salt environment and provides a referential method for an industrial biotechnology and especially for high density culture of lactic acid bacteria under salt stress.

The invention relates to novel metal ion tolerance keratinase and application thereof, and provides keratinase based on metagenome technology excavation and an application method of the keratinase, belonging to the field of industrial biotechnology. The overall length of the keratinase is 1,149bp, and 382 amino acids are encoded; take B.subtilis WB600 as an example, the heterologous expression ofa keratinase gene is successfully realized. The method for obtaining the keratinase gene is convenient and feasible, the gene is subjected to recombined expression, a constructed recombination straincan realize secretory expression of recombined keratinase, the recombined keratinase has relatively good tolerance to metal ions and a surface active agent, and the stability of enzyme is relatively good. Recombinant bacteria are short in fermentation period and suitable for industrial production in large scale. In addition, the enzyme has good reducing capacity and can be applied to research in which a biological method is used for preparing nano-silver particles, and the prepared nano-silver particles have good forms and relatively good bacteriostatic activity.

The invention relates to a novel bionic affinity purification material and application thereof in purifying chitosanase, which belongs to the technical field of industry biology. An affinity ligand ofthe bionic affinity material is chitobiose, a connection arm is cyanuric chloride, and a basic medium is activated agarose 6B. A dissociation constant (Kd) and a maximum combination capacity (Qmax) of the bionic affinity material are respectively 24.2 microgram/mL and 24.1 mg/g. A chitosanase bionic affinity purification method is established by utilizing the bionic affinity material, so that thehigh-purity chitosanase can be produced in high efficiency and low cost, and the industrialized application potential is good.

The invention discloses a promoter for heterologous expression of keratinase, and an application thereof, and belongs to the field of industrial biotechnology. Keratinase recombinant bacteria carrying16 different promoter sequences are successfully constructed, 7 promoter sequences can increase the expression level of keratinase, and the enzyme activity level of a ParpE promoter in transforming the recombinant bacteria is highest, reaches 2605 U/mL, and is 20 times higher than that of control bacteria. The keratinase activity in a fermentation solution in a 5L fermenter reaches up to 7176 U/mL, and is the highest level of recombinant keratinase expression reported in the literature, so the promoter can well serve in practical applications. An effective strategy is provided for high-efficiency expression of keratinase and enzyme production researches. Traditional genetic engineering transformation usually requires a high-throughput screening method and large-scale library screening, solarge workload, long cycle and high cost exist; compared with the traditional method, the reconstruction method in the invention has the advantages of great reduction of the workload, and improvementof the expression efficiency.

The invention relates to the field of industrial biotechnology, and discloses a recombinant Pichia pastoris expressing glucose oxidase fermentation device, comprising a fermenting tank, a nutrient salt feeding bottle and a control cabinet, wherein the fermenting tank is filled with Pichia astoris fermentation broth. The nutrient salt feeding bottle is communicated with the fermenting tank througha feed pump, and the fermenting tank is internally provided with a living cell electrode; the control cabinet is connected with the feed pump and the living cell electrode, and the control cabinet controls the feed pump according to the conductivity data returned by the living cell electrode to feed. The invention also discloses a culturing method of Pichia pastoris, comprising the steps of glycerol batch culture, glycerol fed-batch culture and methanol induction. According to the device and method provided by the invention, the nutrient salt concentration of a culture medium can be monitoredin real time by the living cell electrode, so that the Pichia pastoris G/GMH1 is in an optimal production state, thereby improving the expression efficiency of bacteria expressing glucose oxidase.

The invention belongs to the industrial biotechnology field and provides an actinomycetes culture medium and application thereof. The actinomycetes culture medium consists of the following basic compositions: a yeast extract, peptone, Ca<2+>, Zn<2+>, Cu<2+>, Fe<2+> and a carbon source; the mass ratio of the above compositions is (2-10):(1-8):(0.01-0.2):(0.002-0.03):(0.0002-0.003):(2-14); distilledwater is dissolved and diluted, and the concentration of the yeast extract is controlled to be 2-12 g/L. The actinomycetes culture medium and the application thereof provided by the invention have the benefits that micromonospora and fiberomonas bacterial strains cultured by the actinomycetes culture medium can produce fibrous body analogues; one of enzymes has the activity of hydrolyzing a DAXPC-7 glycosidic bond of a taxol precursor; the enzyme production efficiency is relatively high, so that the synthetic pathway of taxol can be greatly shortened, the cost is reduced, the environmental pollution is reduced, and the like; therefore, the actinomycetes culture medium is an excellent enzyme preparation for preparation of a pharmaceutical intermediate for industrial microbial transformation.

The invention discloses a method for synthesizing natamycin through solid-state fermentation of industrial and agricultural byproducts, and in particular relates to a method for synthesizing the natamycin by taking cheap agricultural and light industrial byproducts as substrates through a solid-state fermentation method, and belongs to the technical field of industrial biotechnologies. A solid-state fermentation culture medium provided by the invention comprises a carbon source, a nitrogen source, an auxiliary carbon source and a fluffy carrier, wherein the mass ratio of the carbon source to the nitrogen source is (1 to 3) to (1 to 3); the adding amount of the auxiliary carbon source is 2 to 5 weight percent; the adding amount of the fluffy carrier is 2 to 5 weight percent; the water content in the culture medium is 50 to 100 percent (v/w); the solid-state fermentation medium provided by the invention is used for culturing streptomyces gilvosporeus and fermenting to produce the natamycin, so that the energy consumption and cost in a fermentation process are greatly reduced; meanwhile, the industrial and agricultural byproducts are effectively utilized.

The invention discloses a high-yield propionic acid propionibacterium jensenii engineering bacterium and application thereof, and belongs to the field of genetic engineering. A molecular method is adopted. The propionibacterium jensenii engineering bacterium serves as a host, and overexpression is carried out on malate dehydrogenase (mdh) from Klebsiella pneumoniae, or overexpression is simultaneously carried out on glycerol dehydrogenase (gldA) from the Klebsiella pneumoniae. Compared with an original strain, the propionic acid yield of recombination and mixing coexpression glycerol dehydrogenase of overexpression malate dehydrogenase and the propionic acid yield of a recombinant strain of the malate dehydrogenase are 36.09 g/L and 39.43 g/L respectively, and are improved by 33.91 % and 46.3 % respectively than that of the original strain. The method provides new ideas for modifying the propionibacterium jensenii and improving productivity of propionic acid fermentation in the industrial biotechnology.

The invention provides a zymogram analysis method for semi-cellulosome analogue and belongs to the technical field of industrial biology. The method disclosed by the invention is characterized in thatthe whole analysis process is composed of three parts: electrophoresis, zymogram analysis and proteinogram analysis. A continuous gradient non-modified gel without concentrated gel is adopted for electrophoresis; an off-on probe substrate is adopted for zymogram analysis; coomassie blue staining or silver staining is adopted for proteinogram analysis. According to the method, once electrophoresisis performed while zymogram and proteinogram information of a block of gel are collected; if multienzyme complex is generated in supernatant of microorganism fermentation liquor can be quickly and effectively judged; the zymogram analysis method is developed for screening microbial strains capable of generating cellulosome compounds and can be applied to the fields of industrial bioconversion, biological energy source, medical intermediate preparation, and the like.

The invention relates to a method of boosting excessive accumulation of pyruvate by adding praline, belonging to directionally changing and optimizing microorganism metabolic function and optimizing fermentation process technical field, which is characterized in that: the torulopsis glabrata strain (T.glabrata) CCTCC M202019 of multiplex vitamin drawback is used as the starting strain, when the concentration of the sodium pyruvate is 0.4mol/L, praline is added into the culture medium and the concentration of the praline in the culture medium is 1g/L, the praline protects the torulopsis glabrata strain cells as compatible material and boosts the CCTCC M202019 to synthesize pyruvate. The method of boosting excessive accumulation of pyruvate by adding praline has the advantages that: the pyruvate fermentation cycle is shortened for 8 hours by adding 1g/L praline into the culture medium, and the pyruvate yield and production intensity is respectively enhanced for 5.4 percent and 18.6 percent; the research result can be applied to the organic acid fermentation and other typical industrial biological process, and provides new technique to industrial biotechnique, in particular the optimization of the fermentation process.

The invention discloses a compound of keratinase and application in industrial production, and belongs to the field of the industrial biotechnology. The invention provides a method in which keratinaseis independently applied to the industrial tanning depilation or is compounded with lipase and amylase to be applied to the industrial tanning depilation, and the beneficial effects that depilation efficiency is high, the action conditions are mild, and the safety performance is good are achieved. The keratinase is adopted and can be used for independently completing sheepskin depilation, the quality of depilated leather is subjected to representation, and the depilation effect is the same as that of commercial enzyme; besides, lipase, amylase and keratinase are provided to perform compound depilation on sheepskin, the three kinds of enzymes are combined to have a collaborative effect on sheepskin depilation, the using amount of the enzymes can be reduced, and the depilation efficiency isimproved. The keratinase and a compounded enzyme preparation of the keratinase have good application prospects in the tanning field.