Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for improving biomass of lactic acid bacteria under high salt condition

A lactic acid bacteria and biomass technology, which is applied in the field of fermentation engineering, can solve the problems of hindering cell density, difficulty in high-density culture of lactic acid bacteria in a highly active lactic acid bacteria starter, and achieves improved resistance to high salt stress, good growth performance, and a simple method. easy effect

Active Publication Date: 2016-08-10
SICHUAN UNIV
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Salt stress also hinders the further increase of cell density, making it more difficult to prepare highly active lactic acid bacteria starter and high-density culture of lactic acid bacteria

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0011] Example 1: When the NaCl concentration in the fermentation medium is 12%, the growth of T.halophilusCGMCC 3792 (Table 1)

[0012] The seed solution was inoculated into the fermentation medium with 0.5g / L proline (addition group) and the fermentation medium without proline (control group) with 5% inoculation amount, and the NaCl concentration was adjusted to 12%. , pH 7.0. After 48h of fermentation, measure the OD 600 The OD value of the sample added with proline was 34.7% higher than that of the sample without proline added.

example 2

[0013] Example 2: When the NaCl concentration in the fermentation medium is 18%, the growth of T.halophilusCGMCC 3792 (Table 1)

[0014] Take the seed liquid and inoculate it into the fermentation medium with 0.5g / L proline (addition group) and the fermentation medium without proline (control group) at an inoculation amount of 5%, and adjust the NaCl concentration to 18%. , pH 7.0. After 48h of fermentation, measure the OD 600 The OD value of the sample added with proline was 30.6% higher than that of the sample without added proline.

[0015] Table 1 Bacterial biomass in high-salt fermentation medium

[0016] .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for improving biomass of lactic acid bacteria under the high salt condition and belongs to the technical field of bioengineering. Proline improves biomass of lactic acid bacteria in a high salt fermentation medium. The method has simple processes, is convenient for operation, effectively improves growth performances of lactic acid bacteria in a high salt environment and provides a referential method for an industrial biotechnology and especially for high density culture of lactic acid bacteria under salt stress.

Description

technical field [0001] The invention relates to a method for increasing the biomass of lactic acid bacteria under high-salt conditions by adding proline, and belongs to the technical field of fermentation engineering. Background technique [0002] Small molecule organic acids such as lactic acid, the metabolite of lactic acid bacteria, can not only produce ester components through condensation reaction with alcohols, endow the product with unique flavor, but also reduce the pH of the system and inhibit harmful microorganisms, such as Escherichia coli, Bacillus subtilis, etc. growth. Therefore, lactic acid bacteria have been widely used in the production of high-salt fermented condiments (soy sauce, fish sauce, soybean paste). In the production of different types of fermented condiments, the salt content is quite different, and the fluctuation range is also different. The high-salt stress environment seriously affects the normal physiological metabolism of lactic acid bacte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/38C12R1/01
Inventor 吴重德何桂强黄钧周荣清
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com