The invention relates to the field of biostimulants and particularly relates to a preparation method for a plant growth stimulant and an application of the plant growth stimulant. The plant growth stimulant is prepared through preparing a seed solution from yellow-ocher streptomyces, inoculating the seed solution to a fermentation tank containing a mixed culture medium of maize meal, soybean meal, glutamic acid, potassium dihydrogen phosphate, sodium chloride, seaweed powder and cerealose which are in a certain ratio, carrying out fermentation, and uniformly mixing fermentation broth of the yellow-ocher streptomyces with multivitamins, multiple amino acids and algal polysaccharides which are in a certain ratio. The plant growth stimulant disclosed by the invention can be added into organic fertilizers, water-soluble fertilizers and microbicides according to a certain ratio so as to carry out synergizing, increase yield, prolong drug effect duration and enhance immunity. The plant growth stimulant disclosed by the invention has the effect of playing a role in stimulating natural courses of plants, including being beneficial to the enhancement of nutrient absorption, nutritional effects, abiotic stress resistance and crop quality, promoting the growth of roots, stems and leaves of crops, and improving the resistance of the crops, can be used for stimulating cell division of plants, promoting formation of chlorophyll and enhancing photosynthesis of the plants and has the aims of promoting growth, advancing ripeness, keeping enough flowers and young fruits, increasing yield, improving crop quality and achieving the yield increase and high yield of the crops.