The present invention provides a preparation composition comprising a solvent, a drug component and a surfactant, the preparation composition is emulsion, surfactant-containing liquid droplets are distributed in the solvent, the volume average particle diameter of the liquid droplet is 100mum or less, the drug component comprises one drug or more drugs for treatment of otitis media, otitis externa, rhinitis, nasosinusitis, lower respiratory tract inflammation, xerostomia, xerophthalmia and dry noses, the surface tension of the preparation composition is less than 60mN / m, and the absolute value of the zeta potential is more than 15mV. The present invention also discloses a preparation method and use of the preparation composition. The preparation composition can relieve the otitis media, the otitis externa, the rhinitis, the nasosinusitis, the lower respiratory tract inflammation, the xerostomia, the xerophthalmia, the dry noses and other symptoms, can be used in the form of sprays or liquid droplets, can be applied to an effective site without use of a propellant or an atomization device, is easy to operate, and can effectively avoid stimulation and injury caused by the propellant and use inconvenience of the atomization device.