78 results about "Nile red" patented technology

The invention discloses a high-throughput screening method of oil-rich microalgae, relates to the field of microalgae biological energy, and aims at solving the problems that an existing microalgae organic solvent extraction method consumes time and labor and requires a large quantity of reagents and samples, a toxic reagent does harm to human body and environment, and the content of oil is hard to measure with high throughput during microalgae screening. The method comprises the following steps of: (1) fetching microalgae and performing enrichment culture for 7-14 days; (II) repeating for twice to three times; (III) after inoculation, culturing for 7-14 days; (IV) diluting and spraying, and culturing until algae colonies appear; (V) choosing large pure algae colonies with a high growth speed, and culturing for 7-14 days; (VI) repeating steps (IV) to (V) for 3-4 times; and (VII) performing ultrasonic breaking, adding a Nile red solution, and selecting an algae strain with the highest fluorescence intensity to obtain an algae strain with a high oil content. The method disclosed by the invention avoids complicated traditional oil detection steps, and overcomes the problem of bottleneck that a quick high-throughput screening method is unavailable in large-scale screening of algae seeds.

The invention belongs to the field of drug screening and particularly relates to a method of creating a zebra fish peristalsis model and screening prokinetic medicaments. The method comprises the following steps of: (1) selecting the zebra fish; (2) inducing the model and treating the compound; (3) quantitatively analyzing through a fluorescence microscope. A fluorescent dye is applied in the invention for the first time to research the gastrointestinal peristalsis conditions, the new research method is a great complement for the existing research models, and the peristalsis model has important value in high throughput screening of the prokinetic compounds due to the maneuverability, non-invasion, simplicity and quickness.

The invention discloses water-dispersible multicolour fluorescent polymer nanoparticles and a preparation method thereof. The water-dispersible multicolour fluorescent polymer nanoparticles are prepared by taking methyl methacrylate as a monomer, taking 4-ethyoxyl-9-allyl-1,8-naphthalimide (EANI), 4-amido-7-nitro-N-allylbenzo[1,2,5] oxadiazole (NBDAA), and Nile red methacrylate (NRME) as polymerizable fluorescent dyes, taking lauryl sodium sulphate (SDS) as a surfactant, and using a one-step miniemulsion polymerization method. The water-dispersible multicolour fluorescent polymer nanoparticles are great in dispersibility in water, quite high in fluorescent brightness, and capable of emitting multicolour fluorescence from blue to red under the excitation of a single wave. The water-dispersible multicolour fluorescent polymer nanoparticles obtained by the preparation method disclosed by the invention are uniform in size, and good in structure and light stability, has a fluorescence emission signal capable of being adjusted for many times in a wide interval under a single excitation wavelength, is simple in synthesis route, convenient to use, suitable for amplification synthesis and actual production applications, and has a great application prospect in the fields of biological encoding and multiple biological analysis.

The invention discloses a two-photon fluorescence probe for detecting peroxynitrite as well as a preparation method and application of the two-photon fluorescence probe. The two-photon fluorescence probe has the structural formula as shown in the description. The two-photon fluorescence probe is named as TPNIR-FP. The two-photon fluorescence probe adopts a Nile red derivative as a fluorescent group and an alpha-keto-amide functional group as an ONOO-reaction group, can rapidly respond to ONOO- (within 10 seconds), and in addition is high in sensitivity and good in selectivity. The two-photon fluorescence probe can be applied to imaging research on ONOO- in antharcycline antibiotic induced cardiac muscle cell and cardiac muscle tissue damage.

The invention provides an enhanced type fluorescent probe for detecting hydrogen sulfide. The fluorescent probe is an NRS fluorescent probe, the structural general formula of the NRS fluorescent probe is shown as (I), the preparation method comprises the following steps: adding nile red, 2,4-dinitrofluorobenzene and potassium carbonate into dimethylformamide, heating to 70-80DEG C, performing reflux reaction for 3 hours, extracting the reaction liquor by dichloromethane and collecting an organic phase, drying the organic phase with anhydrous magnesium sulfate, and purifying through silica column chromatography to obtain a target product. The probe has a mitochondrial location function, can perform real-time and on-site online detection to mitochondria, and can help us to further master the physiological function of the hydrogen sulfide and promote the understanding of physiological action of H2S, and provide a visible detection tool for research and development of novel cardiovascular drugs.

The invention relates to ratio fluorescent nano hydrogel for pH value sensing and a preparation method thereof, belonging to the field of pH value sensing materials. The method comprises the following steps of: weighing polyurethane, bromothymol blue, coumarin 6 and Nile red according to a mass percent of 1:0.05:0.05:0.005; adding the weighed polyurethane to a mixed solution of alcohol and water with a volume ratio that the alcohol to the water is 9:1 to prepare a solution with a concentration of 500ppm; dissolving the bromothymol blue, the coumarin 6 and the Nile red together in the obtained solution and stirring for 1h; dialyzing for 24h in secondary distilled water and changing water every 4h to obtain a hydrogel suspension; and filtering the prepared suspension to obtain a product. The invention overcomes the defect that the detection sensitivity with single fluorescence intensity can be weakened because of the distribution and the concentration of probes and the random drift of an optoelectronic system and is suitable to be used as a pH value detection material in cells.

The invention provides an SCD-phenylenevinylene derivative-Nile red ternary nano supermolecular light harvesting system and a preparation method thereof. The chemical structural formula of the system construction unit is shown in the description. The SCD-phenylenevinylene derivative-Nile red ternary nano supermolecular light harvesting system and the preparation method thereof have the advantages that imidazole-modified phenylenevinylene has good aggregation-induced emission characteristic, aggregation-induced quenching cannot occur under high concentration, and the imidazole-modified phenylenevinylene can be taken as an energy donor; the cyclodextrin can reduce the critical aggregation concentration of the imidazole-modified phenylenevinylene and improve the aggregation-induced emission property of the imidazole-modified phenylenevinylene, so that the light harvesting system has excellent water solubility; hydrophobic dye Nile red can be loaded to the fluorescent nanoparticles constructed by the SCD-imidazole-modified phenylenevinylene, can be taken as a receptor to realize efficient energy transfer and has a relative wide application prospect in simulating natural photosynthesis.

An “in-gel” staining technique for detecting and/or separating proteins during electrophoresis, illustratively as a modification of the standard Laemmli procedure. A fluorescent dye, such as Nile red or Phosphine, is included in the running buffer (mobile phase) which may be a standard Laemmli Tris-Glycine SDS buffer that has been modified to reduce the concentration of detergent (SDS) to less than the typical concentration (0.10% v/v). The fluorescent dye stains proteins during electrophoretic separation. The post-electrophoretic operations are, therefor, reduced and the separated, stained fractions are recoverable for further processing, purifying or analysis.

The invention relates to a biomass energy utilization technology, and aims to provide a method for screening microalgae unicells which grow fast and are high in grease content through a fluorescence microscope. The method for screening the microalgae unicells which grow fast and are high in grease content through the fluorescence microscope includes the following steps: taking a microalgae liquid for unicell separation; enlarging the cultivation to obtain a liquid containing a plurality of unicellular microalgae strains; then inoculating the unicellular microalgae liquid to 96-mesh plates for cultivation until the stable phase; screening the superior microalgae unicells with a high growth rate and dying using Nile red fluorochrome/DMSO; calculating the grease content of the microalgae unicells, so as to screen the superior microalgae unicells with a high grease content. According to the invention, superior algal strains which grow fast and are high grease content can be quickly screened from the algal cells on the 96-well plates in about 10 minutes, which greatly improves the working efficiency of screening target algal strains and reduces labor time and cost.

The invention aims to provide a method and an application for detecting copper ions in a water phase with lipid bilayer-containing organic/inorganic composite fluorescent nanoparticles which are prepared by an organic/inorganic composite lipid and phospholipid. The method employs a principle of fluorescence resonance energy transfer, selects a hydrophobic fluorescent dye-nile red as an energy donor, embeds the nile red in a hydrophobic lipid bilayer, adopts copper ions in a to-be-detected sample as energy acceptors, and mixes amino group-containing phospholipid with the organic/inorganic composite lipid according to a certain proportion to prepare the fluorescent nanoparticles, wherein the amino groups provides binding sites for the copper ions and the fluorescent nanoparticles; detections for the copper ions are realized according to fluorescent changing conditions of a fluorescent nanoparticle solution. The method is simple in operations, has good selectivity and high sensitivity, and can be used in a plurality of fields of environmental science, food science, etc.

The invention discloses a preparation method of a controllable micelle with a visible light and pH responsiveness, and belongs to the polymer field. The preparation method specifically comprises the following steps: subjecting polymerizable monomer dimethylaminoethyl methacrylate to carry out electron transfer and free radical polymerization reactions to polymerize polydimethylaminoethyl methacrylate, then grafting 3-bromoperylene onto the poly(dimethylaminoethyl methacrylate) so as to obtain an amphiphilic polymer with a visible light and pH responsiveness, and making the amphiphilic polymer into micelle with a visible light and pH responsiveness through a micellization process. The polymer micelle is stable at the room temperature, and can load hydrophobic molecules such as Nile red, and the like; the visible light irradiation and pH value can be adjusted to change the micelle morphology, and thus the loaded molecules are released from the micelle; the polymer micelle not only has a pH responsiveness, but also has a visible light responsiveness, and is a novel polymer micelle with a visible light and pH responsiveness. The self-assembly polymer material with a visible light and pH responsiveness and the preparation method thereof have a vast application aspect in the drug control-release field.

The invention discloses a method for mutagenizing and screening microalgae with a high oil yield by ARTP. The method mainly comprises the following two processes: (1) mutagenizing microalgae by usingARTP, and determining the optimal mutagenizing time by adjusting the influence of mutagenizing time on the mutagenizing effect and taking a fatality rate as the basis, and (2) carrying out gradient dilution (capable of efficiently and accurately counting) on the mutated algae liquid at each mutagenesis time, uniformly coating the mutated algae liquid on a solid culture medium, carrying out inverseculture in an illumination incubator for 10 days, selecting the algae colonies with the dark color and larger diameter, inoculating a liquid culture medium with the algae colonies, measuring the absorbance of the algae liquid and the fluorescence value of neutral grease dyed by Nile red to know the cell proliferation rate and grease accumulation condition of microalgae, through comprehensive screening of two basic indexes and the product of the two basic indexes, screening a mutagenic algae strain with a high grease yield, so that the possibility of preparing biodiesel from microalgae is further improved.

The invention discloses preparation of a sericine bound paclitaxel nano-drug sericin-PBLG-PTX-Nile-red and application thereof in tumor tracing and treatment. According to the invention, sericine is adopted as the hydrophilic segment, hydrophobic polypeptide is taken as the hydrophobic segment, and alpha-amino acid-N-carboxyanhydride (NCA) ring-opening reaction is carried out to prepare an amphiphilic sericine polymer. At the same time, amphiphilic sericine is taken as the carrier to load a non-water soluble antitumor drug paclitaxel and a Nile red fluorescent agent by means of hydrophobic interaction, thus obtaining the sericin-PBLG-PTX-Nile-red. The sericin-PBLG-PTX-Nile-red is expected to be applied in the tumor field as a novel controlled release drug with excellent performance and efficient antitumor activity.

The invention relates to preparation and application of a near-infrared and two-photon dual-mode imaging fluorescence probe, and belongs to the technical field of chemicobiology. The near-infrared and two-photon dual-mode imaging fluorescence probe is synthesized by a nile red derivative and biotin which serve as raw materials, and the fluorescence probe is a brown product. The fluorescence probe disclosed by the invention is high in spectral quality, and respectively takes 540 nm and 590 nm as excitation wavelengths; at 660 nm, the maximum emission wavelength appears, and the fluorescence intensity is relatively high. The fluorescence probe further has a targeting effect on tumor cells, and is a targeting infrared fluorescence probe; on one hand, on the premise of not destroying a tissue sample, an in-vivo signal can be detected in real time; on the other hand, enrichment of the signal in a tumor can be enhanced, and the signal-to-noise ratio is increased. The fluorescence probe disclosed by the invention can carry out near infrared imaging and two-photon imaging; by mutual verification of the two modes, the accuracy of a result can be improved.

The invention discloses a method for improving genetic characteristics in diatom oil production, and relates to the genetic breeding method of diatom. The method comprises the following steps: (1) establishing karyogene transformation plasmid; (2) transferring the transformation plasmid into the genome of the diatom to establish a random inserting mutant library; (3) sorting high oil-producing mutant strains in the mutant library through a Nile red fluorescence staining ratio method; (4) measuring triglyceride in the cell, analyzing the fatty acid; and (5) measuring the growth. With adoption of the method, fine varieties can be selected and cultivated by randomly inserting mutation; and the method can be applied to any diatom in which a genetic material transfer system is successfully established; the strains with good comprehensive performance can be successfully sieved easier than gene expression or silence; compared with the methods of chemical mutagenesis and irradiation mutagenesis, the method has no harm to the human body. According to the method, the oil accumulation of the sorted high oil-producing mutant strains is increased, and moreover, the ratio of polyunsaturated fatty acids in the triglyceride can be reduced, so that the triglyceride can be served as the raw material for producing biodiesel without being subject to saturation modification.

A fluorescence-based method for highly sensitive and selective detection of analyte molecules is proposed. The method employs the energy transfer between two or more fluorescent chromophores in a carefully selected polymer matrix. In one preferred embodiment, signal amplification has been achieved in the fluorescent sensing of dimethyl methylphosphonate (DMMP) using two dyes, 3-aminofluoranten (AM) and Nile Red (NR), in a hydrogen bond acidic polymer matrix. The selected polymer matrix quenches the fluorescence of both dyes and shifts dye emission and absorption spectra relative to more inert matrices. Upon DMMP sorption, the AM fluorescence shifts to the red at the same time the NR absorption shifts to the blue, resulting in better band overlap and increased energy transfer between chromophores. In another preferred embodiment, the sensitive material is incorporated into an optical fiber system enabling efficient excitation of the dye and collecting the fluorescent signal form the sensitive material on the remote end of the system. The proposed method can be applied to multichromophore luminescence sensor systems incorporating N-chromophores leading to N-fold signal amplification and improved selectivity. The method can be used in all applications where highly sensitive detection of basic gases, such as dimethyl methylphosphonate (DMMP), Sarin, Soman and other chemical warfare agents having basic properties, is required, including environmental monitoring, chemical industry and medicine.

The invention discloses camouflaging textile dyestuff. The camouflaging textile dyestuff is prepared from, by weight, 25-65 parts of a carbon nano-material, 12-32 parts of benzanthrone, 10-15 parts of an acridine condensed cyclic hydrocarbon, 14-30 parts of squarylium cyanine, 10-20 parts of Nile red and 22-36 parts of auxiliaries, wherein the auxiliaries include an accelerating agent, an oxidizing agent and a dispersing agent. By means of the mode, the camouflaging textile dyestuff has good performance for absorbing infrared rays, is suitable for printing and dyeing infrared camouflage clothes, reduces the probability that a human body object is detected, and improves safety of field detection.

The invention discloses a method for detecting the content of micro-stickies in paper pulp. The method comprises the following steps: firstly, preparing suspension of a paper pulp sample required to be measured according to concentration need, and dispersing uniformly; then selecting corresponding fluorescent dye, taking Nile red dye as an example, and preparing with certain concentration; adding the prepared fluorescent dye into the suspension of the to-be-detected paper pulp sample according to certain proportion; mixing uniformly for a period of time; taking the mixed liquid of a proper amount of suspension of the to-be-detected paper pulp sample and the fluorescent dye, and measuring the fluorescence value by a fluorospectro photometer, thereby obtaining the content of the micro-stickies in the paper pulp according to the measured fluorescence value. The detection method provided by the invention is convenient and rapid; the test results are good in reliability; the content of the stickies are directly displayed in front of operators in a data type; the detection method is convenient to apply in production for regular monitoring.

The invention discloses application of chitosan metal complex particles as a dosage carrier based on active oxygen responsiveness. The weight-average molecular weight of chitosan is 60-250 kDa, the degree of deacetylation is 80-95%, and the metal ions are divalent or trivalent metal ions. According to in-vitro ultraviolet turbidity measurement and nile red in-vitro release test results, it is found that the chitosan metal complex particles obtained through different methods have the good oxidation responsiveness, the oxidation response speeds and in-vitro release efficiency of two particles are remarkably increased on the existence condition of metal ions, and the chitosan metal complex particles are expected to be developed to be the novel drug carrier based on active oxygen intelligent responsiveness.

The invention discloses fluorescence spectrophotometry for detecting the grease content of living algae cells in real time and belongs to methods for rapidly screening the grease content of microalgae plants. According to the fluorescence spectrophotometry for detecting the grease content of the algae cells, microalgae of different species and different shapes is different in structure and shape, experiments and measurement are conducted on the microalgae, and dyeing conditions required by the microalgae of different species and different shapes are different, including selection of the concentration of solutions containing the algae cells, selection of dosage of fluorescence dye, selection of environments for the dyeing process and selection of parameters of fluorescence intensity measurement conditions; the concentration of the algae cells is 1*10<6>/ml-8*10<6>/ml; the volume of algae liquid is 5ml; the solubility of Nile red in the aqueous phase is 0.5-4 microgram/ml; vortex shaking time is 0.5-4 minutes. The fluorescence spectrophotometry has the advantages that complex steps that in traditional dry weight method grease measurement methods, it is required to make dry algae powder and conduct grease extraction and the like are omitted, algae liquid of the living cells is directly utilized for measuring the grease content in real time, rapidly and accurately, and microalgae plants high in grease content can be screened in a high-pass and rapid mode.

The invention discloses a compound fluorescent micro-nano system and a preparation method thereof based on a one-pot process. A biomedical material with the matrix being butyl polyacrylate is constructed, wherein the entrapment component can be selected from fluorescent substances such as rhodamine, coumarin 6, nile red, camptothecin, adriamycin amycin and the like. The system disclosed by the invention is simple in preparation process, mild in condition, green and environment-friendly in process, less in energy consumption, free of pollution of three wastes, radiation, noise and the like and simple and convenient in separation and purification process. The obtained system is stable and easy to store and is a universal process for selectively preparing the compound fluorescent micro-nano system. Based on good biocompatibility and stability of the compound fluorescent micro-nano system as well as the fluorescence imaging and treating functions of the entrapment component, the system is expected to be relatively widely applied in the field of in vivo marking and tracing, biomedical imaging, targeted diagnosis and treat integration, medicine screening and optimization and the like and has good economic and social benefits in the field of life health and personal medical treatment and the like.

The invention provides a decitabine nano carrier and an application thereof in preparing a tumor fluorescence imaging agent. The decitabine nano carrier is prepared by a multiple emulsion method. Bovine hemoglobin, fluorescent dye Nile red, a polylactic acid-glycolic acid copolymer and the like are adopted for emulsification, then wrapping is performed by using chitosan loaded with decitabine andchitosan modified by nitrobenzooxadiazole (NBD), and sterilizing is performed to prepare freeze-dried powder, wherein the freeze-dried powder is dissolved in a water phase for oxygen loading before being used. The decitabine nano carrier is based on 769-P and OS-RC-2 kidney cancer cell lines, and a hypoxia cell model and a tumor-bearing mouse model are constructed. The results show that CNDHNNPs can improve the hypoxia microenvironment and reduce drug efflux, and decitabine can increase uptake of chemotherapeutic drugs and increase sensitization of chemotherapy. Peroxidation of lipid is increased by catalysis, and ferroptosis therapy is combined. The invention provides a novel scheme of hypoxia and hypermethylation combined ferroptosis of targeting tumor for renal cancer treatment, provides a drug delivery system, and realizes tumor fluorescence imaging. The decitabine nano carrier is reasonable in design and high in repeatability.