42 results about "Nile red" patented technology

The invention provides a schizochytrium limacinum mutant strain for producing eicosapentaenoic acid as well as a mutagenesis method and application of the schizochytrium limacinum mutant strain, and belongs to the technical field of microorganisms. In order to culture a new EPA high-yield microbial strain, the method comprises the following steps: firstly, carrying out high-throughput screening based on Nile red staining on schizochytrium limacinum by utilizing a method of combining normal-pressure and room-temperature plasma (ARTP) mutagenesis and sethoxydim pressure, so as to obtain a high-fat-content mutant strain; then the high-fat-content mutant strain is used for diethyl sulfate (DES) mutation breeding, a triclos-2, 2-bipyridine composite plate screening technology, shake flask fermentation and GC quantitative analysis to breed an EPA high-yield strain, and finally the schizochytrium limacinum high-yield mutant strain DBT-64 is obtained. The mutant strain DBT-64 is subjected to passage for many times, the character is stable, the yield of EPA is increased by 490.33%, the economic effect is remarkable, and the mutant strain DBT-64 can be used for commercial production of EPA.

The invention belongs to the technical field of photosensitizer preparation and biological medicine, and particularly relates to a photosensitizer and a preparation method and application thereof. The I-type photosensitizer Nile violet NV is designed and synthesized on the basis of fluorescent dye Nile red through monatomic substitution and structural modification, and the structural formula of the I-type photosensitizer Nile violet NV is shown in the formula I. The photosensitizer based on Nile red can generate O2 <.-> after illumination, and has I-type photodynamic therapy potential; the nanomaterial has a good active oxygen generation capability in tumor cells, and is beneficial to the application of tumor photodynamic therapy. The invention proves that the compound shows an excellent anti-tumor effect in mouse solid tumors and can be widely applied to the field of anti-tumor treatment; meanwhile, the I-type photosensitizer is simple in synthesis method, good in biological safety and remarkable in tumor photodynamic therapy effect, so that the I-type photosensitizer has good practical application value.

The invention relates to development of novel red light and dark red light emitting Nile red derivatives and application of the novel red light and dark red light emitting Nile red derivatives in cell lipid droplet fluorescence imaging, and belongs to the technical field of biological imaging. Chemical names of the fluorescent probes are respectively 9-(4-(diethylamino) phenyl)-5H-benzo [a] phenoxazine-5-ketone, (E)-9-(4-(dimethylamino) styryl)-5H-benzo [a] phenoxazine-5-ketone, (E)-9-(4-(-1-pyrrolidinyl) styryl)-5H-benzo [a] phenoxazine-5-ketone and (E)-9-(4-(diethylamino) styryl)-5H-benzo [a] The invention relates to phenoxazine-5-ketone. The series of cell lipid droplet fluorescence imaging probe molecules prepared by the invention are multifunctional fluorescence imaging probe molecules which have ICT emission characteristics, high labeling selectivity, high light stability, environmental sensitivity and excellent biocompatibility and can be used for confocal imaging.

The invention discloses a microscopic determination method for fiber micro-plastics in a textile aqueous solution, and relates to the technical field of environmental pollutant detection.The method comprises the steps that a water sample is collected; preparing a Nile red staining solution; adding the prepared nile red staining solution into a water sample and carrying out fluorescent staining on the water sample; filtering the water sample subjected to fluorescence dyeing by using a trace glass suction filtration device with a glass fiber filter membrane; washing the glass fiber filter membrane with an acetone solution, and washing the glass fiber filter membrane with third-stage water for multiple times; taking down the washed glass fiber filter membrane, and drying the glass fiber filter membrane in a vacuum oven; and placing the dried glass fiber filter membrane on a microscope objective table, observing fluorescent substances by using a fluorescence microscope, measuring the size and counting. The method has the effects of effectively eliminating the interference of background fluorescence and non-fiber micro-plastics, greatly reducing the omission ratio of the fiber micro-plastics, reducing the determination cost, not needing large instruments, being simple to operate and less in time consumption, and effectively improving the determination efficiency.

The invention relates to a Nile red-based photosensitizer as well as preparation and application thereof, and develops a series of Nile red-based photosensitizers, the structural formula of which is as follows: # imgabs0. By changing the electron donating ability of a donor, the Nile red-based photosensitizers not only have profound influence on the structure of the photosensitizers, but also have important significance on active oxygen generation of the photosensitizers. The NTPA disclosed by the invention has ultrahigh active oxygen generation capacity, has huge potential of resisting multiple drug-resistant bacteria and promoting healing of subcutaneous abscess wounds, and shows the most excellent photodynamic therapy performance. The yield of active oxygen can exceed 470 times within 5 minutes under the irradiation of white light, the NTPA nanoparticles (NPs) show extremely high killing capacity on various drug-resistant bacteria in vitro, the in-vitro bacteriostasis rate can reach 99.9%, and the NTPA nanoparticles also show excellent sterilization and wound healing promoting capacity on the living body level and have huge application prospects.

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

A method for determining the penetration properties of a compound into and / or through a plant leaf wax cuticular layer, said method comprising a) providing a model plant wax layer comprising at least one wax and Nile Red; b) subsequently contacting the model plant wax layer with a composition comprising the compound; and c) monitoring the penetration of the compound into and / or through the model plant wax layer by monitoring the change in Nile Red fluorescence. Furthermore, the use of Nile Red comprised in a wax layer for determining the penetration properties of a compound into and / or through said wax layer is described, and a wax layer deposited onto a solid support, wherein said wax layer comprises at least one plant wax and Nile Red.

The invention relates to the technical field of nano-plastics, and discloses a multi-wavelength excited fluorescent nano-plastic and a synthetic method and application thereof.The synthetic method comprises the steps that an emulsion polymerization method is adopted, styrene serves as a monomer, deionized water serves as a continuous phase, an emulsifier and an initiator are added, a polymerization reaction is conducted, and polystyrene nano-particles are synthesized; the preparation method further comprises the following steps: mixing styrene with other components, and then adding Nile red; and carrying out europium ion doping on the synthesized polystyrene nanoparticles. According to the invention, through dual effects of Nile red fluorescence labeling and europium doping, the imaging signal intensity of the polystyrene nanoparticles in a living body is obviously improved; the europium ions doped by the acetone swelling method enable the migration path and the accumulation process of the nanoparticles in a living body to be more accurately and quantitatively analyzed.

The invention discloses preparation and application of a multicolor nano fluorescent probe based on fluorescent micromolecules FRET, belongs to the technical field of medical reagents, and particularly relates to a preparation method of the nano fluorescent probe. Then adding an organic reagent and a siloxane precursor to prepare an organic phase solution, adding the organic phase solution into an alkaline water phase system to form nano particles, and finally coating the outer layer with silicon dioxide to prepare the nano fluorescent probe. The use volume ratio of the donor fluorescent small molecule solution to the receptor fluorescent small molecule solution is (0.5-1): (0-25). According to the invention, donor fluorescent micromolecules are HMAT blue fluorescent micromolecules, and receptor fluorescent micromolecules comprise BODIPY488 or Nile red.

Disclosed in the present invention are preparation and a use of a fluorescent probe targeting a GPR84 receptor. Specifically, the structure of the fluorescent probe is as shown in formula (I), wherein in the formula, the definitions of substituents are as stated in the description. The compound of formula (I) of the present invention can be used as a fluorescent probe for a GPR84 receptor on a cell membrane surface. The probe can specifically target a GPR84 receptor on a cell membrane surface, and emit specific fluorescence in a fat-soluble environment by means of a Nile red fluorescent group. The probe compound is simple to synthesize and has high yield, can quickly target a corresponding membrane receptor and generate signals, and achieves good specificity and a high signal-to-noise ratio.

The present invention discloses a method for preparing a medical active dye in the field of dye technology. The method comprises the following components: modified hydrotalcite, hydroxyl-modified Nile Red, and water. The medical active dye is obtained by loading the hydroxyl-modified Nile Red onto the modified hydrotalcite; the modified hydrotalcite is a lamellar carrier obtained by modifying the hydrotalcite with γ-aminopropyltriethoxysilane. The present invention proposes that the water solubility of the modified Nile Red is initially improved by modifying the Nile Red, and that the modified hydrotalcite is further used for loading the dye to achieve the technical effects of improved water solubility and luminescence performance.

The present application relates to a kind of lipid droplet targeted AIE molecule in seaweed dyeing imaging and screening etc., the AIE molecule is a kind of aldehyde group substitution triphenylamine skeleton aggregation-induced emission organic fluorescent molecule, it has bright blue-green fluorescence, good biocompatibility, excellent seaweed lipid droplet targeting nature.Compared with commercial probe Nile red, it has better specific marking effect. After using this probe to dye seaweed, the size, content and change of lipid droplet in seaweed can be clearly observed.Combined with flow cytometry screening technology, seaweed with strong fluorescent signal is screened, and seaweed with more and larger lipid droplets is obtained, and the culture result shows that the yield of seaweed biological lipids and the proportion of lipid material in dry weight are significantly improved, about twice of the original culture before screening.Therefore, it has important significance and value in marine microalgae research and biological crude oil production etc.

This invention relates to a Nile Red derivative with photodynamic-photothermal properties, its preparation, and its applications. Two Nile Red derivatives (TPACN and TPAOMCN) were carefully designed and synthesized, with the following structural formulas: Under 660nm laser irradiation, the Nile Red derivatives of this invention can generate a large amount of reactive oxygen species and achieve efficient photothermal conversion, exhibiting powerful photodynamic-photothermal therapeutic effects. They can not only resist multidrug-resistant bacterial infections to promote wound healing but also block the spread of monkeypox virus. The TPAOMCN NFs of this invention can effectively eliminate drug-resistant bacteria in mouse wounds under laser irradiation, regulate inflammatory responses, and promote the healing of infected wounds. In the characteristic pustular lesions of simulated monkeypox, after TPAOMCN NFs combined with laser treatment, the viral titer within the lesions was significantly reduced, the infected lesion tissue was rapidly repaired, and the spread of the virus to healthy mice was effectively blocked, demonstrating great application potential.

The present invention relates to a Nile red-based photosensitizer and its preparation and application. The present invention has developed a series of Nile red-based photosensitizers with the following structural formula: The present invention not only has a profound impact on the structure of the photosensitizer by changing the electron donating ability of the donor, but also has important significance for the generation of active oxygen by the photosensitizer. The NTPA of the present invention has ultra-high active oxygen generation capacity, has great potential to fight multi-drug resistant bacteria and promote the healing of subcutaneous abscess wounds, and exhibits the most excellent photodynamic therapy performance. Under white light irradiation, its active oxygen production can be generated more than 470 times within 5 minutes. NTPA nanoparticles (NPs) show extremely high killing ability against various drug-resistant bacteria in vitro, and its in vitro antibacterial rate can reach 99.9%. It also shows its excellent bactericidal and wound healing ability at the living body level, and has great application prospects.

The invention relates to the technical field of biological medicines, in particular to a spleen-targeted membrane fusion type delivery carrier as well as a preparation method and application thereof. The delivery carrier comprises a dye and lipid nanoparticles, wherein the lipid nanoparticles comprise an ionizable cationic lipid, an auxiliary lipid and a PEG lipid; the dye is selected from one or more of CyBI7, ICG, Nile Red, IR780, IR820, ICG COOH, Nile Blue, ROX alkynine, ROX amine and BODIPY (Boron Dipyrromethene). The delivery carrier has spleen targeting and membrane fusion characteristics, and the utilization rate and expression efficiency of nucleic acid drugs are remarkably improved.

The invention discloses a yellow-light-emitting fluorescent probe with a polarity sensitive characteristic and application thereof in preparation of a cell lipid droplet imaging reagent, and belongs to the technical field of biological imaging. According to the present invention, the fluorescent probe Lipi-Yellow has excellent dyeing selectivity, and under the same dyeing and imaging conditions, the Lipi-Yellow shows the dyeing selectivity more excellent than the dyeing selectivity of the commercial lipid droplet fluorescent probe BODIPY and the dyeing selectivity of the commercial lipid droplet fluorescent probe Nile Red; therefore, the fluorescent probe Lipi-Yellow can be used for specifically marking the lipid droplets. The fluorescence imaging results provide abundant information about the lipid droplets, and the polarity change of the lipid droplets can be deeply understood by virtue of the polarity sensitive characteristic, so that the fluorescent probe Lipi-Yellow is a polarity sensitive lipid droplet fluorescent probe with high light stability and dyeing selectivity; the method can be used for marking lipid droplets in cells and monitoring the polarity change of the lipid droplets before and after oleic acid stimulation, and has a huge application prospect.

The application discloses a method for packaging fluorophores based on tris-dolene nanofilm. First, a nanofilm with tris-dolene as a core segment is prepared through gas-liquid interface assembly and condensation reaction, and is carried on a quartz glass. In a wet state, the pore structure of the nanofilm can be maintained without collapsing through freeze-drying treatment. Then, a fluorophore solution is added dropwise on the nanofilm. After the solvent is naturally evaporated, the fluorophore is uniformly dispersed in the nanofilm, spatial and electronic isolation of the fluorophore is realized, and a flexible and uniform fluorescent nanofilm is obtained. The fluorophore packaged by the method includes coumarin (such as 7-(diethylamino) coumarin-3-carbonitrile and coumarin 6), acridine (such as acridine orange), xanthene (such as rhodamine 6G and rhodamine B) and quinonimine (such as Nile red), etc. In addition, the optical properties and sensing behavior of the obtained fluorescent nanofilm are consistent with the optical properties and sensing behavior of the fluorophore in a solution state. Therefore, the application has universality and fidelity.

The invention discloses a method for rapidly detecting nano plastic in food and environmental samples based on self-made nano magnetic beads, and belongs to the technical field of pollution detection. The method comprises the following steps: firstly, preparing nano magnetic beads, reacting FeCl2 and FeCl3 in an ammonia water environment, washing, activating, and freeze-drying to obtain the nano magnetic beads; preparing a fluorescent nano-plastic standard curve, dyeing by using Nile red and detecting and drawing by using a microplate reader; and finally, adsorbing the nano plastic in the sample by using nano magnetic beads, performing fluorescence labeling after desorption, detecting by using a microplate reader, and substituting into a standard curve to obtain the concentration. The nano magnetic beads are uniform in particle size and good in magnetic performance, the adsorption rate of the nano plastic is larger than or equal to 80%, fluorescence labeling and microplate reader detection are combined, large instruments are not needed, operation is easy and convenient, and the nano magnetic beads are suitable for rapid detection of the nano plastic in food and environmental samples.

This invention discloses a class of environmentally sensitive MrgX2 fluorescent probes, their preparation methods, and applications, belonging to the field of biomedical and chemical probe technology. Using a small molecule compound with MrgX2 receptor antagonistic activity as a recognition backbone, a long-chain polyethylene glycol linker is introduced by recognizing the active O or N sites on the aromatic ring substituents in the backbone molecule, and then covalently coupled to a Nile Red fluorescent chromophore to obtain the environmentally sensitive MrgX2 fluorescent probe. Compared with existing probes, the fluorescence emission of this probe is located in the red light region, which can reduce the interference of autofluorescence in biological samples and improve the detection signal-to-noise ratio. Simultaneously, the long-chain polyethylene glycol linker improves the probe's hydrophilicity and dispersibility, reducing non-specific membrane staining and background adsorption, which is beneficial for achieving stable and specific fluorescent labeling of the MrgX2 receptor on the cell membrane surface, making it suitable for fluorescent labeling and visualization analysis of the MrgX2 receptor in cell models.

Proposed is a method for estimating the mass of microplastics by using fluorescent staining. The method may include staining microplastic samples by using a Nile red solution, and capturing a fluorescent image of each of the stained microplastic samples after classifying the stained microplastic samples by mass. The method may also include analyzing the captured fluorescent image to quantify a fluorescence intensity thereof to build a database (DB) about a correlation between mass and fluorescence intensity of microplastics. The method may further include estimating a mass value of an unknown microplastic sample by comparing a fluorescence intensity of a fluorescent image of the unknown microplastic sample obtained by performing the staining and capturing of the unknown microplastic sample with data stored in the database.

The application discloses a microplastic rapid detection method and system based on a spectrum analysis technology, through development of vacuum filtration and density gradient centrifugation means, efficient separation and concentration of samples are realized; meanwhile, an automatic sample processing system is constructed, automatic equipment of filtering, digestion and separation steps is integrated, and time consumption of manual operation is reduced; in addition, based on a rapid screening technology of Nile red fluorescence dyeing, through specific fluorescent labeling of hydrophobic microplastics, microplastics are quickly positioned by using a fluorescence microscope, manual screening time is reduced, and detection efficiency is improved.

The invention provides a method for efficiently synthesizing and rapidly detecting PHB through organic solid waste bioconversion, and relates to the technical field of organic solid waste treatment and recycling. The method comprises the following steps: carrying out solid-liquid separation on the organic solid waste, inoculating halomonas hydrothermal in the separated liquid, and carrying out oscillation fermentation; the halomonas hydrotherm is Halomonas hydrotherm Slthf2 ATCC (American Type Culture Collection) BAA-800, and the halomonas hydrotherm is Halomonas hydrotherm Slthf2 ATCC BAA-800. According to the method disclosed by the invention, the organic solid waste is used as a substrate for synthesizing the PHAs by microorganisms, and the PHA is efficiently synthesized by using the Halomonas strain Slthf2ATCC BAA-800 under an open condition through process optimization, so that the culture cost is reduced, and the recycling of the organic solid waste is realized. The invention further provides a Nile red fluorescent staining and flora density combination method for rapidly judging the concentration of the PHB.

The invention discloses a method for screening fat-soluble oligopeptides for inhibiting foaming of macrophages. The method comprises the following steps: step 1, selecting fat-soluble oligopeptides from black bean protein hydrolysate to form a theoretical database; the method comprises the following steps: carrying out molecular docking on fat-soluble oligopeptides and key targets of macrophages, and taking primarily screened oligopeptides with higher molecular docking score; and 2, staining lipid droplets in the cells by using Nile red, and further screening the inhibition effect of the oligopeptide by using a fluorescence microplate reader. And re-screening oligopeptides with good inhibition effects according to selection. And 3, detecting CE / TC values of the cells so as to screen the oligopeptide with the best inhibition effect, and finally observing the screening result by oil red O staining. Compared with a traditional oil red O dyeing method, the screening method of the oligopeptide drugs has the advantages of reducing subjectivity, being low in cost and large in screening amount. The invention provides a feasible method for screening oligopeptide drugs.

The present invention provides a kind of microplastic convenient detection method based on composite fluorescent staining, belong to the field of environmental pollutant detection. The method comprises: step one, using hydrogen peroxide with a concentration of 30% to digest the water sample to be detected to obtain a first solution; step two, the first solution is filtered with a filter membrane, microplastics are trapped on the filter membrane, and then rinsed with clean water to obtain the filter membrane after rinsing; step three, 10mL-50mL of a mixed dye solution of Nile red and 4',6-diamidino-2-phenylindole is added to the rinsed filter membrane, after standing for 30min under dark conditions, the dye solution is filtered to obtain a filter membrane with microplastics trapped after dyeing; step four, the filter membrane obtained in step three is transferred to a clean glass culture dish, placed at 45°C and dried for 12h to obtain a dried filter membrane; step five, under a predetermined exposure time, the dried filter membrane is observed and counted using a fluorescence microscope to obtain the content of microplastics in the water sample to be detected.

The invention discloses a nile red derivative fluorescence imaging probe molecule with high lipid droplet specificity and light stability and application of the nile red derivative fluorescence imaging probe molecule in cell lipid droplet fluorescence imaging, and belongs to the technical field of biological imaging. The cell lipid droplet fluorescence imaging probe molecule with high lipid droplet specificity and light stability based on the Nile red has fifteen structures which are respectively called NR-1 to NR-15 for short, and the cell lipid droplet fluorescence imaging probe molecule with the fifteen structures based on the Nile red is of a donor and receptor structure. The invention also discloses an application of the fluorescent probe in cell lipid droplet fluorescence imaging, and the cell disclosed by the invention is a HeLa cell. Experiments prove that the fluorescent probe, especially the NR-11 fluorescent probe, has ultrahigh lipid droplet labeling selectivity due to the environment-sensitive TICT emission characteristic, is a lipid droplet fluorescent probe with the advantages of ultrahigh lipid droplet labeling selectivity, high light stability, excellent biocompatibility and the like, and has a huge application prospect.

The invention belongs to the technical field of environmental monitoring, and particularly relates to a portable micro-plastic fiber in-situ detection method and system integrated with an AI algorithm, an integrally designed portable device (the total weight is less than 2.5 kg, and the working condition of-20 DEG C to 50 DEG C is supported) is adopted, in-situ pretreatment (rough filtration, density separation, Fenton digestion and Nile red dyeing) and high-resolution fluorescence image acquisition are carried out, and the detection accuracy of the micro-plastic fiber is improved. Rapid detection is completed in combination with a built-in improved U-Net AI model; wherein the AI model innovatively introduces a self-supervised regularization and multi-modal feature fusion technology to specially solve the problem of small target recognition under a complex background. As an integrated all-in-one machine, the AI algorithm unit and the portable hardware are deeply integrated, the whole detection process only needs 20 minutes, the recognition accuracy is greater than or equal to 85%, and the equipment cost is less than 0.4 thousand yuan. The device is particularly suitable for in-situ detection of a western complex environment, micro-plastic concentration and particle size distribution can be rapidly output through integrated equipment, and accurate data support is provided for western micro-plastic pollution abatement.

The application belongs to the technical field of photosensitizer preparation and biological medicine, and particularly relates to a photosensitizer, a preparation method and application thereof. The photosensitizer based on the fluorescent dye Nile Red can produce O2 ·‑ under light, has the potential of type I photodynamic therapy, has good active oxygen production capacity in tumor cells, and is helpful to the application of tumor photodynamic therapy. The application proves that the photosensitizer exhibits excellent antitumor effect in a mouse solid tumor, can be widely applied to the field of antitumor treatment, and has good practical application value due to the simple synthesis method, good biological safety and significant tumor photodynamic therapy effect of the type I photosensitizer.

This invention relates to the field of bioimaging technology and discloses fluorescent imaging probe molecules derived from Nile Red, possessing excellent labeling ability and high photostability, as well as reagents for lipid droplet fluorescence imaging. The probes are named 9-diethylamino-6-(2-trifluoromethylphenyl)-5H-benzo[a]phenoxazine-5-one and 9-diethylamino-6-(4-trifluoromethylphenyl)-5H-benzo[a]phenoxazine-5-one, abbreviated as NR-1 and NR-2. Experiments have confirmed that the fluorescent probes NR-1 and NR-2 of this invention not only have photophysical properties very similar to Nile Red, but also exhibit significantly improved lipid droplet labeling ability (5-fold increase in signal-to-noise ratio and 15-fold increase in staining brightness compared to Nile Red staining) and photostability. This invention also discloses the application of these fluorescent probes in cell lipid droplet confocal microscopy, SIM super-resolution, and tissue imaging reagents. NR-1 and NR-2 are expected to replace Nile Red as a new generation of widely used lipid droplet fluorescent probes.

The invention discloses an integrated analysis method for enrichment and Raman detection of micro-plastics in biological tissues, and belongs to the technical field of enrichment extraction and detection of micro-plastics. The method comprises the following steps: pre-treating biological tissues through a digestion solution, and filtering a tissue sample digestion solution through a silver filter membrane; dyeing by using a Nile red standard stock solution, and observing and counting the micro-plastic particles by using a fluorescence microscope; all Raman spectrograms of suspected micro-plastic particles enriched on the silver filter membrane are paired with a standard substance reference Raman database on the basis of KnowItAll software. The method provided by the invention has high efficiency and high recovery rate, can accurately detect the MPs component in the tissue in a short time, so that the detection precision is improved, the cost is lower, and the method can be used for a long time, so that the detection cost is reduced; the effective dyeing of the MPs can be realized, so that the fluorescence intensity characterization of the dyed MPs can be stably and accurately observed.

The invention provides an adjustable lanthanide series supramolecular cascade panchromatic light-emitting assembly material as well as a preparation method and application thereof, an adjustable lanthanide series supramolecular cascade full-spectrum light-emitting system is constructed by co-assembling an organic antenna molecule / lanthanide series ion complex, hectorite, Nile blue / Nile red and rhodamine 800 (Rh800), the energy transfer efficiency is high, and the material is suitable for large-scale production. And full-spectrum adjustable light emission can be realized. The assembly can be used as intelligent anti-counterfeiting ink, and multi-layer information encryption is realized through multi-color light-emitting coding.