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81 results about "Nile red" patented technology
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Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment can be intensely fluorescent, with varying colours from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages). The dye is highly solvatochromic and its emission and excitation wavelength both shift depending on solvent polarity and in polar media will hardly fluoresce at all.
Fluorescent probes for labelling and/or detecting lipid droplets in cells as well as preparation and applications of fluorescent probes
InactiveCN108069902ARaw materials are cheap and easy to getSpecific recognition abilityOrganic chemistryFluorescence/phosphorescenceFluorophoreLipid Body
The invention belongs to the field of biological analysis and detection and relates to fluorescent probes for labelling and / or detecting lipid droplets in cells as well as preparation and applicationsof the fluorescent probes. The probes are synthesized with 4-bromo-1,8-naphthalic anhydride as an initial material and take 1,8-naphthalimide as a fluorophore, a C12 alkyl long chain as a specific lipid droplet positioning group and an N-containing group on the 4th site of a naphthalene ring as a fluorescence enhanced and stabilized group. The probes are simple to prepare and have high yield. Compared with existing Nile red and boron dipyrromethene lipid droplet fluorescent probes, the probes have high light stability and large stokes shift and are more suitable for fluorescence imaging. Theprobes have very important application value in cell labelling fluorescent imaging and biomedical fields.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Amino acid-fluorophore compound and application thereof
ActiveCN102603695AOrganic chemistryMicrobiological testing/measurementNaphthalene diimideAbnormal expression
The invention relates to an amino acid-fluorophore compound and application of the compound, wherein the general structural formula of the amino acid-fluorophore compound is (I), (II), (III), and (IV) or a free form thereof, wherein R1 is selected from various amino acid side chains, preferably methyl, propyl, 2-methylthio ethyl, benzyl, phenylethyl, 2-cyclohexyl ethyl, 4-isopropyl phenyl and 4-dimethyl amino phenyl; R2 is selected from various fluorophores, preferably 7-hydroxyl coumarin, naphthalimide fluorophores, Nile red series and Cy fluorophores; and X is C or N. Fluorescent probe molecules can be used for detecting the activity (enzyme level and cell level) of aminopeptidase N, can be used as a probe tool for detecting the tissue distribution of aminopeptidase N and tumor tissue imaging, and can be used as a diagnosis tool for various diseases due to abnormal expression of aminopeptidase N. In addition, the preparation method of the compound has the advantages of mild reaction conditions, easily-accessible and cheap raw materials and simplicity in operation and after-treatment.
Owner:SHANDONG UNIV
High-throughput screening method of oil-rich microalgae
InactiveCN103146582AAvoid detection stepsSolve bottlenecksUnicellular algaeMicroorganism based processesHuman bodyHigh-Throughput Screening Methods
The invention discloses a high-throughput screening method of oil-rich microalgae, relates to the field of microalgae biological energy, and aims at solving the problems that an existing microalgae organic solvent extraction method consumes time and labor and requires a large quantity of reagents and samples, a toxic reagent does harm to human body and environment, and the content of oil is hard to measure with high throughput during microalgae screening. The method comprises the following steps of: (1) fetching microalgae and performing enrichment culture for 7-14 days; (II) repeating for twice to three times; (III) after inoculation, culturing for 7-14 days; (IV) diluting and spraying, and culturing until algae colonies appear; (V) choosing large pure algae colonies with a high growth speed, and culturing for 7-14 days; (VI) repeating steps (IV) to (V) for 3-4 times; and (VII) performing ultrasonic breaking, adding a Nile red solution, and selecting an algae strain with the highest fluorescence intensity to obtain an algae strain with a high oil content. The method disclosed by the invention avoids complicated traditional oil detection steps, and overcomes the problem of bottleneck that a quick high-throughput screening method is unavailable in large-scale screening of algae seeds.
Owner:HARBIN INST OF TECH
Method of creating zebra fish peristalsis model and screening prokinetic medicaments
ActiveCN103301480AEasy to manufactureFast preparationIn-vivo testing preparationsHigh-Throughput Screening MethodsFluorescence microscope
The invention belongs to the field of drug screening and particularly relates to a method of creating a zebra fish peristalsis model and screening prokinetic medicaments. The method comprises the following steps of: (1) selecting the zebra fish; (2) inducing the model and treating the compound; (3) quantitatively analyzing through a fluorescence microscope. A fluorescent dye is applied in the invention for the first time to research the gastrointestinal peristalsis conditions, the new research method is a great complement for the existing research models, and the peristalsis model has important value in high throughput screening of the prokinetic compounds due to the maneuverability, non-invasion, simplicity and quickness.
Owner:HANGZHOU HUANTE BIOLOGICAL TECH CO LTD
Water-dispersible multicolour fluorescent polymer nanoparticles and preparation method thereof
InactiveCN103497273AHigh fluorescence brightnessImprove structural stabilityLuminescent compositionsMethacrylateActive agent
The invention discloses water-dispersible multicolour fluorescent polymer nanoparticles and a preparation method thereof. The water-dispersible multicolour fluorescent polymer nanoparticles are prepared by taking methyl methacrylate as a monomer, taking 4-ethyoxyl-9-allyl-1,8-naphthalimide (EANI), 4-amido-7-nitro-N-allylbenzo[1,2,5] oxadiazole (NBDAA), and Nile red methacrylate (NRME) as polymerizable fluorescent dyes, taking lauryl sodium sulphate (SDS) as a surfactant, and using a one-step miniemulsion polymerization method. The water-dispersible multicolour fluorescent polymer nanoparticles are great in dispersibility in water, quite high in fluorescent brightness, and capable of emitting multicolour fluorescence from blue to red under the excitation of a single wave. The water-dispersible multicolour fluorescent polymer nanoparticles obtained by the preparation method disclosed by the invention are uniform in size, and good in structure and light stability, has a fluorescence emission signal capable of being adjusted for many times in a wide interval under a single excitation wavelength, is simple in synthesis route, convenient to use, suitable for amplification synthesis and actual production applications, and has a great application prospect in the fields of biological encoding and multiple biological analysis.
Owner:HUNAN UNIV OF SCI & TECH
Two-photon fluorescence probe for detecting peroxynitrite as well as preparation method and application of two-photon fluorescence probe
ActiveCN108164494AIncrease penetration depthReduce self-absorptionOrganic chemistryFluorescence/phosphorescencePeroxynitriteStructural formula
The invention discloses a two-photon fluorescence probe for detecting peroxynitrite as well as a preparation method and application of the two-photon fluorescence probe. The two-photon fluorescence probe has the structural formula as shown in the description. The two-photon fluorescence probe is named as TPNIR-FP. The two-photon fluorescence probe adopts a Nile red derivative as a fluorescent group and an alpha-keto-amide functional group as an ONOO-reaction group, can rapidly respond to ONOO- (within 10 seconds), and in addition is high in sensitivity and good in selectivity. The two-photon fluorescence probe can be applied to imaging research on ONOO- in antharcycline antibiotic induced cardiac muscle cell and cardiac muscle tissue damage.
Owner:SHANDONG NORMAL UNIV
Preparation method and applications of enhanced type fluorescent probe for detecting hydrogen sulfide
InactiveCN104479669AGood optical stabilityImprove solubilityOrganic chemistryFluorescence/phosphorescencePotassium carbonateNile red
The invention provides an enhanced type fluorescent probe for detecting hydrogen sulfide. The fluorescent probe is an NRS fluorescent probe, the structural general formula of the NRS fluorescent probe is shown as (I), the preparation method comprises the following steps: adding nile red, 2,4-dinitrofluorobenzene and potassium carbonate into dimethylformamide, heating to 70-80DEG C, performing reflux reaction for 3 hours, extracting the reaction liquor by dichloromethane and collecting an organic phase, drying the organic phase with anhydrous magnesium sulfate, and purifying through silica column chromatography to obtain a target product. The probe has a mitochondrial location function, can perform real-time and on-site online detection to mitochondria, and can help us to further master the physiological function of the hydrogen sulfide and promote the understanding of physiological action of H2S, and provide a visible detection tool for research and development of novel cardiovascular drugs.
Owner:LIAONING UNIVERSITY
Ratio fluorescent nano hydrogel for pH value sensing and preparation method thereof
InactiveCN101762572AThe sensing signal is stable and reliableEfficient use ofMaterial analysis by observing effect on chemical indicatorFluorescence/phosphorescenceBromothymol blueAlcohol
The invention relates to ratio fluorescent nano hydrogel for pH value sensing and a preparation method thereof, belonging to the field of pH value sensing materials. The method comprises the following steps of: weighing polyurethane, bromothymol blue, coumarin 6 and Nile red according to a mass percent of 1:0.05:0.05:0.005; adding the weighed polyurethane to a mixed solution of alcohol and water with a volume ratio that the alcohol to the water is 9:1 to prepare a solution with a concentration of 500ppm; dissolving the bromothymol blue, the coumarin 6 and the Nile red together in the obtained solution and stirring for 1h; dialyzing for 24h in secondary distilled water and changing water every 4h to obtain a hydrogel suspension; and filtering the prepared suspension to obtain a product. The invention overcomes the defect that the detection sensitivity with single fluorescence intensity can be weakened because of the distribution and the concentration of probes and the random drift of an optoelectronic system and is suitable to be used as a pH value detection material in cells.
Owner:BEIJING JIAOTONG UNIV
Sulphonated cyclodextrin (SCD)-phenylenevinylene derivative-Nile red ternary nano supermolecular light harvesting system and preparation method thereof
InactiveCN106967419ALower critical aggregation concentrationGood water solubilityLuminescent compositionsSolubilityHigh concentration
Owner:NANKAI UNIV
In-gel fluorescent protein staining technique
InactiveUS20060144708A1Reduces post-electrophoretic process timeManipulation can be minimizedElectrolysis componentsVolume/mass flow measurementStaining techniqueGlycine
An “in-gel” staining technique for detecting and / or separating proteins during electrophoresis, illustratively as a modification of the standard Laemmli procedure. A fluorescent dye, such as Nile red or Phosphine, is included in the running buffer (mobile phase) which may be a standard Laemmli Tris-Glycine SDS buffer that has been modified to reduce the concentration of detergent (SDS) to less than the typical concentration (0.10% v / v). The fluorescent dye stains proteins during electrophoretic separation. The post-electrophoretic operations are, therefor, reduced and the separated, stained fractions are recoverable for further processing, purifying or analysis.
Owner:KITZLER JEFFREY +2
Polymerizable liquid crystal composition, polarized light-emitting coating material, novel naphtholactam derivative novel coumarin derivative, novel nile red derivative, and novel anthracene derivative
ActiveUS20130334461A1Improve polarizationLiquid crystal compositionsOrganic chemistryAnthraceneNaphtholactam
Provided are a polymerizable liquid crystal composition, a coating material, a medium, and a polarizing device each produced using a polymerizable liquid crystal compound and a colorant and each capable of producing polarized light suitable for polarizing devices, and also a novel naphtholactam derivative, a novel coumarin derivative, a novel Nile Red derivative, and a novel anthracene derivative each suitable for use as the colorant. Specifically provided are a polymerizable liquid crystal composition containing (A) at least one liquid crystal compound having a polymerizable functional group, (B) at least one colorant, and (C) a polymerization initiator, and a novel naphtholactam derivative of formula (IV′), a novel coumarin derivative of formula (VI′), a novel Nile Red derivative of formula (VII′), and a novel anthracene derivative of formula (VIII′) each suitable for use as the colorant (B).
Owner:ADEKA CORP +1
Polymerizable liquid crystal composition, polarized light-emitting coating material, novel naphtholactam derivative, novel coumarin derivative, novel nile red derivative, and novel anthracene derivative
InactiveCN103403127AHigh polarized luminescenceHigh degree of polarizationLiquid crystal compositionsOrganic chemistryAnthraceneNaphtholactam
The present invention provides a polymerizable liquid crystal composition that demonstrates favorable polarized light emission for a polarized light element, the composition using a polymerizable liquid crystal compound and colorant; also provided are a coating material, a medium, and a polarized light-element. Furthermore the present invention provides a favorable novel naphtholactam derivative, novel coumarin derivative, novel Nile red derivative, and novel anthracene derivative as the colorant. Specifically, provided is a polymerizable liquid crystal composition containing (A) at least one type of liquid crystal compound having a polymerizable functional group, (B) at least one type of colorant, and (C) a polymerization initiator; further provided are a novel naphthyl and derivative expressed by general formula (IV'), a novel coumarin derivative expressed by general formula (VI'), a novel Nile red derivative expressed by general formula (VII'), or a novel anthracene derivative expressed by general formula (VIII'), which are favorable as the colorant of component (B).
Owner:ADEKA CORP
Marine pollutant micro-plastic dyeing method
The invention relates to a marine pollutant micro-plastic dyeing method, comprising the steps of mixing Coomassie brilliant blue and methylene blue in an organic solvent to form a dye solution; selecting from marine pollutants, a mixture containing micro=plastic; dyeing the mixture with the dye solution, and keeping vibrating for dyeing for a predetermined period of time. The marine pollution micro-plastic dyeing method has the advantages that Coomassie brilliant blue and methylene blue are mixed in an organic solvent to obtain the dye solution, marine pollutants are dyed via the dye solution,dyeing time is short, dyeing acts fast, and dyeing is convenient and fast and is simple to perform. Compared with traditional high-cost Nile red, Coomassie brilliant blue and methylene blue are lowerin cost and are applicable to wide researches on marine pollutant micro-plastics.
Owner:HAINAN TROPICAL OCEAN UNIV
Method of signal amplification in multi-chromophore luminescence sensors
InactiveUS6686206B2Improve performanceSimple and efficient of detectionMaterial analysis by observing effect on chemical indicatorChemiluminescene/bioluminescenceChemical industryDimethyl methylphosphonate
A fluorescence-based method for highly sensitive and selective detection of analyte molecules is proposed. The method employs the energy transfer between two or more fluorescent chromophores in a carefully selected polymer matrix. In one preferred embodiment, signal amplification has been achieved in the fluorescent sensing of dimethyl methylphosphonate (DMMP) using two dyes, 3-aminofluoranthene (AM) and Nile Red (NR), in a hydrogen bond acidic polymer matrix. The selected polymer matrix quenches the fluorescence of both dyes and shifts dye emission and absorption spectra relative to more inert matrices. Upon DMMP sorption, the AM fluorescence shifts to the red at the same time the NR absorption shifts to the blue, resulting in better band overlap and increased energy transfer between chromophores. In another preferred embodiment, the sensitive material is incorporated into an optical fiber system enabling efficient excitation of the dye and collecting the fluorescent signal form the sensitive material on the remote end of the system. The proposed method can be applied to multichromophore luminescence sensor systems incorporating N-chromophores leading to N-fold signal amplification and improved selectivity. The method can be used in all applications where highly sensitive detection of basic gases, such as dimethyl methylphosphonate (DMMP), Sarin, Soman and other chemical warfare agents having basic properties, is required, including environmental monitoring, chemical industry and medicine.
Owner:ALTAIR CENT
Method for screening microalgae unicells which grow fast and are high in grease content through fluorescence microscope
ActiveCN104568881AImprove work efficiencyReduce labor time costsFluorescence/phosphorescenceFluorescence microscopeCell separation
The invention relates to a biomass energy utilization technology, and aims to provide a method for screening microalgae unicells which grow fast and are high in grease content through a fluorescence microscope. The method for screening the microalgae unicells which grow fast and are high in grease content through the fluorescence microscope includes the following steps: taking a microalgae liquid for unicell separation; enlarging the cultivation to obtain a liquid containing a plurality of unicellular microalgae strains; then inoculating the unicellular microalgae liquid to 96-mesh plates for cultivation until the stable phase; screening the superior microalgae unicells with a high growth rate and dying using Nile red fluorochrome / DMSO; calculating the grease content of the microalgae unicells, so as to screen the superior microalgae unicells with a high grease content. According to the invention, superior algal strains which grow fast and are high grease content can be quickly screened from the algal cells on the 96-well plates in about 10 minutes, which greatly improves the working efficiency of screening target algal strains and reduces labor time and cost.
Owner:ZHEJIANG UNIV
Method for detecting copper ions with lipid bilayer-containing organic/inorganic composite fluorescent nanoparticles
InactiveCN102818790ARealize detectionSimple preparation processFluorescence/phosphorescenceEnergy transferBinding site
The invention aims to provide a method and an application for detecting copper ions in a water phase with lipid bilayer-containing organic / inorganic composite fluorescent nanoparticles which are prepared by an organic / inorganic composite lipid and phospholipid. The method employs a principle of fluorescence resonance energy transfer, selects a hydrophobic fluorescent dye-nile red as an energy donor, embeds the nile red in a hydrophobic lipid bilayer, adopts copper ions in a to-be-detected sample as energy acceptors, and mixes amino group-containing phospholipid with the organic / inorganic composite lipid according to a certain proportion to prepare the fluorescent nanoparticles, wherein the amino groups provides binding sites for the copper ions and the fluorescent nanoparticles; detections for the copper ions are realized according to fluorescent changing conditions of a fluorescent nanoparticle solution. The method is simple in operations, has good selectivity and high sensitivity, and can be used in a plurality of fields of environmental science, food science, etc.
Owner:HARBIN INST OF TECH
Method for rapid and efficient breeding of oil-producing strains and application thereof
InactiveCN108690814ASimple and efficient mutagenesis methodGood genetic stabilityFungiMutant preparationBiotechnologyLiquid medium
The invention provides a method for rapid and efficient breeding of oil-producing strains. Firstly, Mutagenesis of Trichosporon cutaneum is carried out by an atmospheric pressure and room temperatureplasma mutagenesis instrument. Then, the mutant Trichosporon cutaneum is inoculated on a flat medium containing sesamol. Strains with vigorous growth are selected and inoculated into 48-well liquid mediums, and high throughput screening is carried out on the cultured strains by 96-well plate Nile red fluorescence staining to obtain mutant strains with fast proliferation and high oil yield. Finally, oil composition is detected by gas chromatography. After mutagenesis and screening, a strain A1 with high oil yield is screened out. Compared with the original strain, the biomass, oil yield and oilproductivity of the strain A1 are increased by 41.6%, 120.3% and 58.8% respectively. The fatty acid composition of the strain A1 is the same as that of the original strain by gas chromatography composition analysis, including palmitic acid, oleic acid, linoleic acid, linolenic acid, etc. Combining ARTP plasma mutagenesis technology with malic enzyme inhibitor and Nile red fluorescent staining, high-throughput screening is realized and a strain with high oil yield is successfully bred.
Owner:CHINA THREE GORGES UNIV
Polymerizable liquid crystal composition, polarized light-emitting coating material, novel naphtholactam derivative novel coumarin derivative, novel nile red derivative, and novel anthracene derivative
ActiveUS9475991B2Improve polarizationLiquid crystal compositionsOrganic chemistryAnthraceneNaphtholactam
Owner:ADEKA CORP +1
Preparation method of self-assembly polymer material with visible light and pH responsiveness
InactiveCN103509145AAchieve controlled releaseAvoid strong destructive light sourcesMethacrylateAtom-transfer radical-polymerization
Owner:UNIV OF SCI & TECH BEIJING
Method for mutagenizing and screening microalgae with high oil yield by ARTP
InactiveCN110885816AEfficient screeningHigh fat contentMutant preparationMicroorganism based processesBiodieselAlgae
The invention discloses a method for mutagenizing and screening microalgae with a high oil yield by ARTP. The method mainly comprises the following two processes: (1) mutagenizing microalgae by usingARTP, and determining the optimal mutagenizing time by adjusting the influence of mutagenizing time on the mutagenizing effect and taking a fatality rate as the basis, and (2) carrying out gradient dilution (capable of efficiently and accurately counting) on the mutated algae liquid at each mutagenesis time, uniformly coating the mutated algae liquid on a solid culture medium, carrying out inverseculture in an illumination incubator for 10 days, selecting the algae colonies with the dark color and larger diameter, inoculating a liquid culture medium with the algae colonies, measuring the absorbance of the algae liquid and the fluorescence value of neutral grease dyed by Nile red to know the cell proliferation rate and grease accumulation condition of microalgae, through comprehensive screening of two basic indexes and the product of the two basic indexes, screening a mutagenic algae strain with a high grease yield, so that the possibility of preparing biodiesel from microalgae is further improved.
Owner:XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
Preparation of sericine bound paclitaxel nano-drug sericin-PBLG-PTX-Nile-red and application thereof in tumor tracing and treatment
InactiveCN106963732AEfficient and fast accessSafe entryOrganic active ingredientsEmulsion deliverySericinWilms' tumor
The invention discloses preparation of a sericine bound paclitaxel nano-drug sericin-PBLG-PTX-Nile-red and application thereof in tumor tracing and treatment. According to the invention, sericine is adopted as the hydrophilic segment, hydrophobic polypeptide is taken as the hydrophobic segment, and alpha-amino acid-N-carboxyanhydride (NCA) ring-opening reaction is carried out to prepare an amphiphilic sericine polymer. At the same time, amphiphilic sericine is taken as the carrier to load a non-water soluble antitumor drug paclitaxel and a Nile red fluorescent agent by means of hydrophobic interaction, thus obtaining the sericin-PBLG-PTX-Nile-red. The sericin-PBLG-PTX-Nile-red is expected to be applied in the tumor field as a novel controlled release drug with excellent performance and efficient antitumor activity.
Owner:胡彦锋 +2
Near-infrared and two-photon dual-mode imaging fluorescence probe, as well as preparation and application thereof
InactiveCN106753337AGood spectral propertiesHigh fluorescence intensityOrganic chemistryFluorescence/phosphorescenceDual modeIn vivo
The invention relates to preparation and application of a near-infrared and two-photon dual-mode imaging fluorescence probe, and belongs to the technical field of chemicobiology. The near-infrared and two-photon dual-mode imaging fluorescence probe is synthesized by a nile red derivative and biotin which serve as raw materials, and the fluorescence probe is a brown product. The fluorescence probe disclosed by the invention is high in spectral quality, and respectively takes 540 nm and 590 nm as excitation wavelengths; at 660 nm, the maximum emission wavelength appears, and the fluorescence intensity is relatively high. The fluorescence probe further has a targeting effect on tumor cells, and is a targeting infrared fluorescence probe; on one hand, on the premise of not destroying a tissue sample, an in-vivo signal can be detected in real time; on the other hand, enrichment of the signal in a tumor can be enhanced, and the signal-to-noise ratio is increased. The fluorescence probe disclosed by the invention can carry out near infrared imaging and two-photon imaging; by mutual verification of the two modes, the accuracy of a result can be improved.
Owner:UNIV OF JINAN
Method for improving genetic characteristics in diatom oil production
InactiveCN102827856AEasy to shapeNo harmUnicellular algaeMicroorganism based processesBiodieselStaining
The invention discloses a method for improving genetic characteristics in diatom oil production, and relates to the genetic breeding method of diatom. The method comprises the following steps: (1) establishing karyogene transformation plasmid; (2) transferring the transformation plasmid into the genome of the diatom to establish a random inserting mutant library; (3) sorting high oil-producing mutant strains in the mutant library through a Nile red fluorescence staining ratio method; (4) measuring triglyceride in the cell, analyzing the fatty acid; and (5) measuring the growth. With adoption of the method, fine varieties can be selected and cultivated by randomly inserting mutation; and the method can be applied to any diatom in which a genetic material transfer system is successfully established; the strains with good comprehensive performance can be successfully sieved easier than gene expression or silence; compared with the methods of chemical mutagenesis and irradiation mutagenesis, the method has no harm to the human body. According to the method, the oil accumulation of the sorted high oil-producing mutant strains is increased, and moreover, the ratio of polyunsaturated fatty acids in the triglyceride can be reduced, so that the triglyceride can be served as the raw material for producing biodiesel without being subject to saturation modification.
Owner:INST OF AQUATIC LIFE ACAD SINICA +1
Method of signal amplification in multi-chromophore luminescence sensors
InactiveUS20030032197A1Improve chemical performanceSimple and efficient of detectionMaterial analysis by observing effect on chemical indicatorChemiluminescene/bioluminescenceChemical industryDimethyl methylphosphonate
A fluorescence-based method for highly sensitive and selective detection of analyte molecules is proposed. The method employs the energy transfer between two or more fluorescent chromophores in a carefully selected polymer matrix. In one preferred embodiment, signal amplification has been achieved in the fluorescent sensing of dimethyl methylphosphonate (DMMP) using two dyes, 3-aminofluoranten (AM) and Nile Red (NR), in a hydrogen bond acidic polymer matrix. The selected polymer matrix quenches the fluorescence of both dyes and shifts dye emission and absorption spectra relative to more inert matrices. Upon DMMP sorption, the AM fluorescence shifts to the red at the same time the NR absorption shifts to the blue, resulting in better band overlap and increased energy transfer between chromophores. In another preferred embodiment, the sensitive material is incorporated into an optical fiber system enabling efficient excitation of the dye and collecting the fluorescent signal form the sensitive material on the remote end of the system. The proposed method can be applied to multichromophore luminescence sensor systems incorporating N-chromophores leading to N-fold signal amplification and improved selectivity. The method can be used in all applications where highly sensitive detection of basic gases, such as dimethyl methylphosphonate (DMMP), Sarin, Soman and other chemical warfare agents having basic properties, is required, including environmental monitoring, chemical industry and medicine.
Owner:ALTAIR CENT
Camouflaging textile dyestuff
InactiveCN105507019APromote absorptionReduce the probability of detectionDyeing processInfraredNile red
The invention discloses camouflaging textile dyestuff. The camouflaging textile dyestuff is prepared from, by weight, 25-65 parts of a carbon nano-material, 12-32 parts of benzanthrone, 10-15 parts of an acridine condensed cyclic hydrocarbon, 14-30 parts of squarylium cyanine, 10-20 parts of Nile red and 22-36 parts of auxiliaries, wherein the auxiliaries include an accelerating agent, an oxidizing agent and a dispersing agent. By means of the mode, the camouflaging textile dyestuff has good performance for absorbing infrared rays, is suitable for printing and dyeing infrared camouflage clothes, reduces the probability that a human body object is detected, and improves safety of field detection.
Owner:WUXI KELAIXIN ELECTROMECHANICAL MFG
Method for detecting content of micro-stickies in paper pulp
InactiveCN103743610AThe detection method is convenient and fastClear resultMaterial analysis by observing effect on chemical indicatorPreparing sample for investigationProcess engineeringFluorochrome Dye
The invention discloses a method for detecting the content of micro-stickies in paper pulp. The method comprises the following steps: firstly, preparing suspension of a paper pulp sample required to be measured according to concentration need, and dispersing uniformly; then selecting corresponding fluorescent dye, taking Nile red dye as an example, and preparing with certain concentration; adding the prepared fluorescent dye into the suspension of the to-be-detected paper pulp sample according to certain proportion; mixing uniformly for a period of time; taking the mixed liquid of a proper amount of suspension of the to-be-detected paper pulp sample and the fluorescent dye, and measuring the fluorescence value by a fluorospectro photometer, thereby obtaining the content of the micro-stickies in the paper pulp according to the measured fluorescence value. The detection method provided by the invention is convenient and rapid; the test results are good in reliability; the content of the stickies are directly displayed in front of operators in a data type; the detection method is convenient to apply in production for regular monitoring.
Owner:SHAANXI UNIV OF SCI & TECH
Method for rapidly testing freshness of rice
InactiveCN107402207AEasy to detectReduce testing costsMaterial analysis by observing effect on chemical indicatorColor/spectral properties measurementsBromothymol blueOctaethylporphycene
The invention discloses a method for rapidly testing freshness of rice. The method adopts the following two agents A and B; the reagent A contains sodium methylate, sodium chloride, bromothymol blue, methyl red and a buffer liquid; the reagent B contains octaethylporphine, protoporphyrin IX dimethyl ester, Nile red and a solvent; and colors of a sample to be tested, a standard product corresponding to the reagent A and a standard product corresponding to the reagent B are compared and scored respectively, and then a final freshness value of 0.43*score of reagent A + 0.61*score of reagent B is obtained. The method is convenient and rapid in detection, high in detection accuracy and convenient in production application.
Owner:COFCO CHENGDU GRAIN & OIL IND
Application of chitosan metal complex particles as dosage carrier based on active oxygen responsiveness
InactiveCN105688222AGood oxidation responseStrong oxidation responsePowder deliveryInorganic non-active ingredientsUltravioletOxygen
The invention discloses application of chitosan metal complex particles as a dosage carrier based on active oxygen responsiveness. The weight-average molecular weight of chitosan is 60-250 kDa, the degree of deacetylation is 80-95%, and the metal ions are divalent or trivalent metal ions. According to in-vitro ultraviolet turbidity measurement and nile red in-vitro release test results, it is found that the chitosan metal complex particles obtained through different methods have the good oxidation responsiveness, the oxidation response speeds and in-vitro release efficiency of two particles are remarkably increased on the existence condition of metal ions, and the chitosan metal complex particles are expected to be developed to be the novel drug carrier based on active oxygen intelligent responsiveness.
Owner:GUANGDONG PHARMA UNIV
Fluorescence spectrophotometry for detecting grease content of living algae cells in real time
InactiveCN105548116ACalculation of fat contentReal-time quick filterFluorescence/phosphorescenceSolubilityDry weight
The invention discloses fluorescence spectrophotometry for detecting the grease content of living algae cells in real time and belongs to methods for rapidly screening the grease content of microalgae plants. According to the fluorescence spectrophotometry for detecting the grease content of the algae cells, microalgae of different species and different shapes is different in structure and shape, experiments and measurement are conducted on the microalgae, and dyeing conditions required by the microalgae of different species and different shapes are different, including selection of the concentration of solutions containing the algae cells, selection of dosage of fluorescence dye, selection of environments for the dyeing process and selection of parameters of fluorescence intensity measurement conditions; the concentration of the algae cells is 1*10<6> / ml-8*10<6> / ml; the volume of algae liquid is 5ml; the solubility of Nile red in the aqueous phase is 0.5-4 microgram / ml; vortex shaking time is 0.5-4 minutes. The fluorescence spectrophotometry has the advantages that complex steps that in traditional dry weight method grease measurement methods, it is required to make dry algae powder and conduct grease extraction and the like are omitted, algae liquid of the living cells is directly utilized for measuring the grease content in real time, rapidly and accurately, and microalgae plants high in grease content can be screened in a high-pass and rapid mode.
Owner:CHINA UNIV OF MINING & TECH
Compound fluorescent micro-nano system and preparation method thereof based on one-pot process
InactiveCN104877669AImprove bioavailabilitySimple manufacturing processMaterial nanotechnologyLuminescent compositionsMicro nanoBiocompatibility Testing
The invention discloses a compound fluorescent micro-nano system and a preparation method thereof based on a one-pot process. A biomedical material with the matrix being butyl polyacrylate is constructed, wherein the entrapment component can be selected from fluorescent substances such as rhodamine, coumarin 6, nile red, camptothecin, adriamycin amycin and the like. The system disclosed by the invention is simple in preparation process, mild in condition, green and environment-friendly in process, less in energy consumption, free of pollution of three wastes, radiation, noise and the like and simple and convenient in separation and purification process. The obtained system is stable and easy to store and is a universal process for selectively preparing the compound fluorescent micro-nano system. Based on good biocompatibility and stability of the compound fluorescent micro-nano system as well as the fluorescence imaging and treating functions of the entrapment component, the system is expected to be relatively widely applied in the field of in vivo marking and tracing, biomedical imaging, targeted diagnosis and treat integration, medicine screening and optimization and the like and has good economic and social benefits in the field of life health and personal medical treatment and the like.
Owner:WENZHOU INST OF BIOMATERIALS & ENG
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