24 results about "Nile red" patented technology

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

A method for determining the penetration properties of a compound into and / or through a plant leaf wax cuticular layer, said method comprising a) providing a model plant wax layer comprising at least one wax and Nile Red; b) subsequently contacting the model plant wax layer with a composition comprising the compound; and c) monitoring the penetration of the compound into and / or through the model plant wax layer by monitoring the change in Nile Red fluorescence. Furthermore, the use of Nile Red comprised in a wax layer for determining the penetration properties of a compound into and / or through said wax layer is described, and a wax layer deposited onto a solid support, wherein said wax layer comprises at least one plant wax and Nile Red.

The invention discloses preparation and application of a multicolor nano fluorescent probe based on fluorescent micromolecules FRET, belongs to the technical field of medical reagents, and particularly relates to a preparation method of the nano fluorescent probe. Then adding an organic reagent and a siloxane precursor to prepare an organic phase solution, adding the organic phase solution into an alkaline water phase system to form nano particles, and finally coating the outer layer with silicon dioxide to prepare the nano fluorescent probe. The use volume ratio of the donor fluorescent small molecule solution to the receptor fluorescent small molecule solution is (0.5-1): (0-25). According to the invention, donor fluorescent micromolecules are HMAT blue fluorescent micromolecules, and receptor fluorescent micromolecules comprise BODIPY488 or Nile red.

The present invention discloses a method for preparing a medical active dye in the field of dye technology. The method comprises the following components: modified hydrotalcite, hydroxyl-modified Nile Red, and water. The medical active dye is obtained by loading the hydroxyl-modified Nile Red onto the modified hydrotalcite; the modified hydrotalcite is a lamellar carrier obtained by modifying the hydrotalcite with γ-aminopropyltriethoxysilane. The present invention proposes that the water solubility of the modified Nile Red is initially improved by modifying the Nile Red, and that the modified hydrotalcite is further used for loading the dye to achieve the technical effects of improved water solubility and luminescence performance.

The present application relates to a kind of lipid droplet targeted AIE molecule in seaweed dyeing imaging and screening etc., the AIE molecule is a kind of aldehyde group substitution triphenylamine skeleton aggregation-induced emission organic fluorescent molecule, it has bright blue-green fluorescence, good biocompatibility, excellent seaweed lipid droplet targeting nature.Compared with commercial probe Nile red, it has better specific marking effect. After using this probe to dye seaweed, the size, content and change of lipid droplet in seaweed can be clearly observed.Combined with flow cytometry screening technology, seaweed with strong fluorescent signal is screened, and seaweed with more and larger lipid droplets is obtained, and the culture result shows that the yield of seaweed biological lipids and the proportion of lipid material in dry weight are significantly improved, about twice of the original culture before screening.Therefore, it has important significance and value in marine microalgae research and biological crude oil production etc.

This invention relates to a Nile Red derivative with photodynamic-photothermal properties, its preparation, and its applications. Two Nile Red derivatives (TPACN and TPAOMCN) were carefully designed and synthesized, with the following structural formulas: Under 660nm laser irradiation, the Nile Red derivatives of this invention can generate a large amount of reactive oxygen species and achieve efficient photothermal conversion, exhibiting powerful photodynamic-photothermal therapeutic effects. They can not only resist multidrug-resistant bacterial infections to promote wound healing but also block the spread of monkeypox virus. The TPAOMCN NFs of this invention can effectively eliminate drug-resistant bacteria in mouse wounds under laser irradiation, regulate inflammatory responses, and promote the healing of infected wounds. In the characteristic pustular lesions of simulated monkeypox, after TPAOMCN NFs combined with laser treatment, the viral titer within the lesions was significantly reduced, the infected lesion tissue was rapidly repaired, and the spread of the virus to healthy mice was effectively blocked, demonstrating great application potential.

The present invention relates to a Nile red-based photosensitizer and its preparation and application. The present invention has developed a series of Nile red-based photosensitizers with the following structural formula: The present invention not only has a profound impact on the structure of the photosensitizer by changing the electron donating ability of the donor, but also has important significance for the generation of active oxygen by the photosensitizer. The NTPA of the present invention has ultra-high active oxygen generation capacity, has great potential to fight multi-drug resistant bacteria and promote the healing of subcutaneous abscess wounds, and exhibits the most excellent photodynamic therapy performance. Under white light irradiation, its active oxygen production can be generated more than 470 times within 5 minutes. NTPA nanoparticles (NPs) show extremely high killing ability against various drug-resistant bacteria in vitro, and its in vitro antibacterial rate can reach 99.9%. It also shows its excellent bactericidal and wound healing ability at the living body level, and has great application prospects.

The invention relates to the technical field of biological medicines, in particular to a spleen-targeted membrane fusion type delivery carrier as well as a preparation method and application thereof. The delivery carrier comprises a dye and lipid nanoparticles, wherein the lipid nanoparticles comprise an ionizable cationic lipid, an auxiliary lipid and a PEG lipid; the dye is selected from one or more of CyBI7, ICG, Nile Red, IR780, IR820, ICG COOH, Nile Blue, ROX alkynine, ROX amine and BODIPY (Boron Dipyrromethene). The delivery carrier has spleen targeting and membrane fusion characteristics, and the utilization rate and expression efficiency of nucleic acid drugs are remarkably improved.

The application discloses a method for packaging fluorophores based on tris-dolene nanofilm. First, a nanofilm with tris-dolene as a core segment is prepared through gas-liquid interface assembly and condensation reaction, and is carried on a quartz glass. In a wet state, the pore structure of the nanofilm can be maintained without collapsing through freeze-drying treatment. Then, a fluorophore solution is added dropwise on the nanofilm. After the solvent is naturally evaporated, the fluorophore is uniformly dispersed in the nanofilm, spatial and electronic isolation of the fluorophore is realized, and a flexible and uniform fluorescent nanofilm is obtained. The fluorophore packaged by the method includes coumarin (such as 7-(diethylamino) coumarin-3-carbonitrile and coumarin 6), acridine (such as acridine orange), xanthene (such as rhodamine 6G and rhodamine B) and quinonimine (such as Nile red), etc. In addition, the optical properties and sensing behavior of the obtained fluorescent nanofilm are consistent with the optical properties and sensing behavior of the fluorophore in a solution state. Therefore, the application has universality and fidelity.

The invention discloses a method for rapidly detecting nano plastic in food and environmental samples based on self-made nano magnetic beads, and belongs to the technical field of pollution detection. The method comprises the following steps: firstly, preparing nano magnetic beads, reacting FeCl2 and FeCl3 in an ammonia water environment, washing, activating, and freeze-drying to obtain the nano magnetic beads; preparing a fluorescent nano-plastic standard curve, dyeing by using Nile red and detecting and drawing by using a microplate reader; and finally, adsorbing the nano plastic in the sample by using nano magnetic beads, performing fluorescence labeling after desorption, detecting by using a microplate reader, and substituting into a standard curve to obtain the concentration. The nano magnetic beads are uniform in particle size and good in magnetic performance, the adsorption rate of the nano plastic is larger than or equal to 80%, fluorescence labeling and microplate reader detection are combined, large instruments are not needed, operation is easy and convenient, and the nano magnetic beads are suitable for rapid detection of the nano plastic in food and environmental samples.

This invention discloses a class of environmentally sensitive MrgX2 fluorescent probes, their preparation methods, and applications, belonging to the field of biomedical and chemical probe technology. Using a small molecule compound with MrgX2 receptor antagonistic activity as a recognition backbone, a long-chain polyethylene glycol linker is introduced by recognizing the active O or N sites on the aromatic ring substituents in the backbone molecule, and then covalently coupled to a Nile Red fluorescent chromophore to obtain the environmentally sensitive MrgX2 fluorescent probe. Compared with existing probes, the fluorescence emission of this probe is located in the red light region, which can reduce the interference of autofluorescence in biological samples and improve the detection signal-to-noise ratio. Simultaneously, the long-chain polyethylene glycol linker improves the probe's hydrophilicity and dispersibility, reducing non-specific membrane staining and background adsorption, which is beneficial for achieving stable and specific fluorescent labeling of the MrgX2 receptor on the cell membrane surface, making it suitable for fluorescent labeling and visualization analysis of the MrgX2 receptor in cell models.

Proposed is a method for estimating the mass of microplastics by using fluorescent staining. The method may include staining microplastic samples by using a Nile red solution, and capturing a fluorescent image of each of the stained microplastic samples after classifying the stained microplastic samples by mass. The method may also include analyzing the captured fluorescent image to quantify a fluorescence intensity thereof to build a database (DB) about a correlation between mass and fluorescence intensity of microplastics. The method may further include estimating a mass value of an unknown microplastic sample by comparing a fluorescence intensity of a fluorescent image of the unknown microplastic sample obtained by performing the staining and capturing of the unknown microplastic sample with data stored in the database.

The application discloses a microplastic rapid detection method and system based on a spectrum analysis technology, through development of vacuum filtration and density gradient centrifugation means, efficient separation and concentration of samples are realized; meanwhile, an automatic sample processing system is constructed, automatic equipment of filtering, digestion and separation steps is integrated, and time consumption of manual operation is reduced; in addition, based on a rapid screening technology of Nile red fluorescence dyeing, through specific fluorescent labeling of hydrophobic microplastics, microplastics are quickly positioned by using a fluorescence microscope, manual screening time is reduced, and detection efficiency is improved.

The invention provides a method for efficiently synthesizing and rapidly detecting PHB through organic solid waste bioconversion, and relates to the technical field of organic solid waste treatment and recycling. The method comprises the following steps: carrying out solid-liquid separation on the organic solid waste, inoculating halomonas hydrothermal in the separated liquid, and carrying out oscillation fermentation; the halomonas hydrotherm is Halomonas hydrotherm Slthf2 ATCC (American Type Culture Collection) BAA-800, and the halomonas hydrotherm is Halomonas hydrotherm Slthf2 ATCC BAA-800. According to the method disclosed by the invention, the organic solid waste is used as a substrate for synthesizing the PHAs by microorganisms, and the PHA is efficiently synthesized by using the Halomonas strain Slthf2ATCC BAA-800 under an open condition through process optimization, so that the culture cost is reduced, and the recycling of the organic solid waste is realized. The invention further provides a Nile red fluorescent staining and flora density combination method for rapidly judging the concentration of the PHB.

The invention belongs to the technical field of environmental monitoring, and particularly relates to a portable micro-plastic fiber in-situ detection method and system integrated with an AI algorithm, an integrally designed portable device (the total weight is less than 2.5 kg, and the working condition of-20 DEG C to 50 DEG C is supported) is adopted, in-situ pretreatment (rough filtration, density separation, Fenton digestion and Nile red dyeing) and high-resolution fluorescence image acquisition are carried out, and the detection accuracy of the micro-plastic fiber is improved. Rapid detection is completed in combination with a built-in improved U-Net AI model; wherein the AI model innovatively introduces a self-supervised regularization and multi-modal feature fusion technology to specially solve the problem of small target recognition under a complex background. As an integrated all-in-one machine, the AI algorithm unit and the portable hardware are deeply integrated, the whole detection process only needs 20 minutes, the recognition accuracy is greater than or equal to 85%, and the equipment cost is less than 0.4 thousand yuan. The device is particularly suitable for in-situ detection of a western complex environment, micro-plastic concentration and particle size distribution can be rapidly output through integrated equipment, and accurate data support is provided for western micro-plastic pollution abatement.

The application belongs to the technical field of photosensitizer preparation and biological medicine, and particularly relates to a photosensitizer, a preparation method and application thereof. The photosensitizer based on the fluorescent dye Nile Red can produce O2 ·‑ under light, has the potential of type I photodynamic therapy, has good active oxygen production capacity in tumor cells, and is helpful to the application of tumor photodynamic therapy. The application proves that the photosensitizer exhibits excellent antitumor effect in a mouse solid tumor, can be widely applied to the field of antitumor treatment, and has good practical application value due to the simple synthesis method, good biological safety and significant tumor photodynamic therapy effect of the type I photosensitizer.

This invention relates to the field of bioimaging technology and discloses fluorescent imaging probe molecules derived from Nile Red, possessing excellent labeling ability and high photostability, as well as reagents for lipid droplet fluorescence imaging. The probes are named 9-diethylamino-6-(2-trifluoromethylphenyl)-5H-benzo[a]phenoxazine-5-one and 9-diethylamino-6-(4-trifluoromethylphenyl)-5H-benzo[a]phenoxazine-5-one, abbreviated as NR-1 and NR-2. Experiments have confirmed that the fluorescent probes NR-1 and NR-2 of this invention not only have photophysical properties very similar to Nile Red, but also exhibit significantly improved lipid droplet labeling ability (5-fold increase in signal-to-noise ratio and 15-fold increase in staining brightness compared to Nile Red staining) and photostability. This invention also discloses the application of these fluorescent probes in cell lipid droplet confocal microscopy, SIM super-resolution, and tissue imaging reagents. NR-1 and NR-2 are expected to replace Nile Red as a new generation of widely used lipid droplet fluorescent probes.

The invention provides an adjustable lanthanide series supramolecular cascade panchromatic light-emitting assembly material as well as a preparation method and application thereof, an adjustable lanthanide series supramolecular cascade full-spectrum light-emitting system is constructed by co-assembling an organic antenna molecule / lanthanide series ion complex, hectorite, Nile blue / Nile red and rhodamine 800 (Rh800), the energy transfer efficiency is high, and the material is suitable for large-scale production. And full-spectrum adjustable light emission can be realized. The assembly can be used as intelligent anti-counterfeiting ink, and multi-layer information encryption is realized through multi-color light-emitting coding.

The invention discloses a preparation method and application of bicolor carboxyl functionalized polystyrene nano-microspheres, acrylic acid is introduced in the synthesis process of polystyrene microspheres to realize carboxyl functionalized modification on the surfaces of the microspheres, and the carboxylated polystyrene nano-microspheres are dyed and subjected to fluorescent coordination through Nile red and europium chloride hexahydrate to obtain the bicolor carboxyl functionalized polystyrene nano-microspheres. Finally, the two-color carboxyl functionalized polystyrene nano-microspheres with red and red fluorescence are obtained. The bi-color carboxyl functionalized polystyrene nano-microsphere prepared by the method has stable fluorescence performance and good dispersity, is expected to show good application prospect and development potential in the frontier fields of biomedical diagnosis, immunochromatographic analysis, wearable detection devices and the like.

The invention discloses a rapid micro-plastic detection method and system based on a spectral analysis technology, and the method comprises the steps: achieving the efficient separation and concentration of a sample through the development of vacuum filtration and density gradient centrifugation means; meanwhile, an automatic sample treatment system is constructed, and automatic equipment integrating filtering, digestion and separation steps is adopted, so that the time consumed by manual operation is reduced; besides, based on a rapid screening technology of Nile red fluorescent staining, through specific fluorescence labeling of hydrophobic micro-plastics, the micro-plastics are rapidly positioned by using a fluorescence microscope, so that the manual screening time is shortened, and the detection efficiency is improved.

The invention relates to the field of fluorescence spectrum microscopic imaging, in particular to a fluorescence microscopic imaging method for polarity of intracellular lipid droplets, which comprises the following steps: selecting a lipid polarity sensitive fluorescent probe Nile red to mark intracellular lipid droplets; controlling the imaging system to continuously scan and irradiate the sample under a plurality of different excitation wavelengths, and cooperating with the camera to acquire an excitation spectrum fluorescence image sequence; an excitation spectrum signal corresponding to each pixel is constructed, discrete Fourier transform is carried out on the excitation spectrum signal, a harmonic component is extracted, and therefore high-dimensional spectrum data is mapped to a two-dimensional phasor space; further carrying out visual color coding analysis on the spectral heterogeneity of the lipid droplets by utilizing the characteristic that a phasor angle corresponds to spectrum movement aiming at the characteristic analysis of a Nile red non-fixed reference spectrum; and finally reconstructing to obtain a spatial distribution map reflecting the polar heterogeneity of the lipid droplets. The method effectively solves the problem that the traditional method is difficult to process the continuous change of the spectrum, and the heterogeneity of the polarity of the lipid droplet in the cell is displayed at high resolution.

This invention discloses a phenothiazine-based near-infrared lipid droplet fluorescent dye, its preparation method, and its applications, belonging to the field of fluorescence sensing and detection technology. The phenothiazine-based near-infrared lipid droplet fluorescent dye is compound II with the following structural formula. In a low-polarity environment, the emission wavelength of this phenothiazine-based near-infrared lipid droplet fluorescent dye is located in the near-infrared region, exhibiting a large Stokes shift and strong fluorescence intensity. It can be used for specific imaging of lipid droplets within living cells, demonstrating strong specificity for lipid droplets and enabling imaging analysis of intracellular lipid droplet division and lipid migration. Leveraging the advantages of near-infrared emission, this dye can be used for in situ imaging of mouse breast cancer. Furthermore, the near-infrared lipid droplet fluorescent dye of this invention exhibits strong stability and significantly superior photobleaching resistance compared to Nile Red.

The invention belongs to the field of bioengineering, and particularly relates to a recombinant strain for high-yield EPA (eicosapentaenoic acid) and application thereof. According to the invention, a long-chain elongation factor coding gene in a Schizochride sp. Genome is deleted by using a homologous recombination technology, and synthesis from EPA (eicosapentaenoic acid) to DHA (docosahexaenoic acid) is cut off. However, the fermentation concentration of the total fat is greatly reduced. Therefore, ARTP mutagenesis is carried out on the basis, and Nile red dyeing is utilized to carry out high-throughput screening of total lipid high-yield strains. And finally, the mutant strain with high total lipid yield and high EPA (eicosapentaenoic acid) proportion is selected.

The invention discloses a method for detecting microplastics from earthworm tissues, and relates to the technical field of detection, and the method comprises the following steps: S1, obtaining an earthworm sample without excrement in intestinal tracts; s2, taking an earthworm sample, cutting the earthworm sample into pieces, soaking the earthworm sample in KOH, performing digestion in a shaking table, taking out the earthworm sample every half an hour, and performing ultrasonic digestion for 3 minutes; s3, carrying out vacuum filtration on the digestion solution to a polycarbonate nuclear track etching filter membrane; s4, putting the filter membrane into a centrifugal tube, adding a saturated ZnCl2 solution, and performing ultrasonic treatment; taking out the filter membrane, washing the filter membrane with a saturated salt solution, and centrifuging the washing liquid; s5, performing vacuum filtration on the supernatant to a new polycarbonate nuclear track etching filter membrane; s6, drying the new filter membrane in a drying oven overnight; and S7, carrying out fluorescent staining with a Nile red reagent and counting. KOH is selected as a digestion solution, ultrasonic treatment is added in the digestion process, the tissue breaking process is greatly accelerated, the digestion time is greatly shortened, the digestion effect is good, a large amount of foam cannot be generated, and the digestion rate can reach 98.1 + / -1.1% within 3 h.