Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for detecting copper ions with lipid bilayer-containing organic/inorganic composite fluorescent nanoparticles

A fluorescent nanometer and particle detection technology, which is applied in the intersection of environmental science and biomaterial science, can solve problems such as instability limiting practical application, and achieve the effects of simple operation, mild reaction conditions and simple preparation process.

Inactive Publication Date: 2012-12-12
HARBIN INST OF TECH
View PDF5 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liposomes have been used as drug carriers in the biomedical field, but their instability has greatly limited their practical application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for detecting copper ions with lipid bilayer-containing organic/inorganic composite fluorescent nanoparticles
  • Method for detecting copper ions with lipid bilayer-containing organic/inorganic composite fluorescent nanoparticles
  • Method for detecting copper ions with lipid bilayer-containing organic/inorganic composite fluorescent nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0030] This embodiment is the selection of organic / inorganic composite lipids and 1,2-diglyceride-3-phosphatidylethanolamine (DMPE) in different molar ratios to prepare fluorescent nanoparticles and the most stable ratio, the preparation process of fluorescent nanoparticles Such as figure 1 , the specific method is as follows:

[0031] 1. Preparation of nanoparticles by film hydration method

[0032] Organic / inorganic composite lipids and phospholipids (DMPE) were mixed in six molar ratios (1.5:1, 2:1, 2.5:1, 3:1, 4:1, 5:1) and packed in a pear-shaped bottle In chloroform, dissolve it with chloroform, add an appropriate amount of Nile Red methanol solution, and then use a rotary evaporator to remove the organic solvent and form a thin film on the bottom of the bottle, add deionized water, and hydrate in a water bath at 51°C for 30 minutes, then Sonicate in a water bath for 5 minutes, and finally sonicate with a probe for 3 minutes to prepare nanoparticles with a particle siz...

specific Embodiment approach 2

[0041] Particle size determination and characterization of fluorescent nanoparticles prepared by mixing organic / inorganic composite lipids and phospholipids at a ratio of 2.5:1. The particle size of the nanoparticles was determined with a dynamic light scattering instrument. Add 2ml of nanoparticle dispersion into the plastic cuvette used for measurement, select an argon ion laser, measure the angle at 90°, and measure the particle size and particle size distribution range at 25°C. Nanoparticles were characterized by transmission electron microscopy. Take appropriate amount of nanoparticle dispersion liquid and drop it on the copper grid, and let it dry naturally at room temperature, so that the particles were concentrated and deposited on the copper grid, and then stained with a dye for 18 minutes. After drying, the transmission electron microscope was observed. , the result is as figure 2 .

[0042] The results in this embodiment all prove that the particle size of the pr...

specific Embodiment approach 3

[0043] Determination of adsorption capacity of nanoparticles to copper ions and verification of Nile Red being successfully embedded in nanoparticles.

[0044] 1. Determination of the ability of nanoparticles to adsorb copper ions.

[0045] Thanks to NH 2 The nitrogen atom has a lone pair of electrons, and the copper ion has an empty electron orbit (4s, 4p), so they can form a coordination bond, which forms a copper ammonium complex ion. The specific method is as follows: 10 concentrations of copper ion solutions were prepared between 0 and 6 μM, and the aforementioned thin-film hydration method was used to prepare nanoparticles without embedding Nile Red, and copper ion solutions of different concentrations were added and It carries out ultraviolet absorption measurement, simultaneously a nanoparticle dispersion liquid and 6 μ M copper ion solution are used as contrast, image 3 A is the ultraviolet absorption spectrum of 6μM copper ions. It can be seen from the figure that...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention aims to provide a method and an application for detecting copper ions in a water phase with lipid bilayer-containing organic / inorganic composite fluorescent nanoparticles which are prepared by an organic / inorganic composite lipid and phospholipid. The method employs a principle of fluorescence resonance energy transfer, selects a hydrophobic fluorescent dye-nile red as an energy donor, embeds the nile red in a hydrophobic lipid bilayer, adopts copper ions in a to-be-detected sample as energy acceptors, and mixes amino group-containing phospholipid with the organic / inorganic composite lipid according to a certain proportion to prepare the fluorescent nanoparticles, wherein the amino groups provides binding sites for the copper ions and the fluorescent nanoparticles; detections for the copper ions are realized according to fluorescent changing conditions of a fluorescent nanoparticle solution. The method is simple in operations, has good selectivity and high sensitivity, and can be used in a plurality of fields of environmental science, food science, etc.

Description

technical field [0001] The invention belongs to the interdisciplinary field of environmental science and biomaterial science, and relates to a method for detecting copper ions by using organic / inorganic composite fluorescent nanoparticles containing lipid bilayers. Background technique [0002] Metal ions play an important role in the fields of environmental science and biology. Among them, copper ions are necessary for human health. It promotes the synthesis of hemoglobin, is a component of many enzymes in metabolism, and is also related to the growth and development of the human body and the immune system. closely related. However, short-term exposure to excessive copper can cause gastrointestinal dysfunction, and prolonged exposure to excessive copper can cause liver and kidney damage. When the water contains copper, it can corrode galvanized pipes, iron and aluminum products. When it is higher than 5mg / L, the water will produce color and bitter taste; when it is higher ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64
Inventor 岳秀丽景元淼梁晓龙戴志飞
Owner HARBIN INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products