40 results about "Fluorescent nanoparticles" patented technology

The present disclosure relates to an azide-modified fluorescent π-conjugated polymer and a DNA-modified π-conjugated fluorescent nanoparticle-based protein detection imaging method capable of signal rapid amplification and high throughput. The present disclosure prepares oligonucleotide-modified fluorescent π-conjugated polymers (OCPNs) based on the azide-modified fluorescent π-conjugated polymer. The OCPNs have ultrahigh fluorescence luminance and extremely high detection sensitivity, and can complete detection and imaging of targets in different abundances without the need of an additional fluorescence signal amplification step. In addition, a rapid and specific targeting effect can be achieved with an extremely small dosage and an entire staining and imaging process can be shortened, so that the OCPNs achieve an extremely high imaging efficiency during multi-color imaging and thus exhibit great application prospects.

The application provides a fluorescent sensor based on a tripod [5] arene, which is obtained by self-assembly complexation of a host of a tripod [5] arene TP5 and a guest of a quaternary ammonium salt in an H2O-DMSO system. A supramolecular system is constructed by host-guest interaction between the tripod [5] arene TP5 and the amphiphilic guest TPN, and then PbS fluorescent nanoparticles are generated in situ under ion induction. Fluorescence enhancement and high-sensitivity detection (LOD=7.00*10 2+ -6 M) of Pb ‑8 are realized. In addition, the fluorescent supramolecular polymer network based on the PbS fluorescent nanoparticles can be successfully applied to in-situ detection of Pb 2+ in cells.

The invention relates to the technical field of biology, and particularly discloses a cronobacter sakazakii nucleic acid aptamer and application thereof in preparation of a biosensor. According to the invention, a nucleic acid aptamer with a DNA sequence as shown in SEQ ID No: 1 is designed for Cronobacter sakazakii MipA protein, and 5'terminal amino is modified. The aptamer can be used for constructing a fluorescence quenching type biosensor, and comprises a fluorescent probe (fluorescent nanoparticles synthesized by citric acid diammonium, thiourea, tetramethyl orthosilicate and cerium dioxide, and a first aptamer modified by carboxyl) and a quenching probe (citric acid reduced gold nanoparticles, a second aptamer modified by sulfydryl, SEQ ID No: 2). Compared with a traditional method, the screening efficiency is remarkably improved, and the detection time is lt; the method has the advantages of high sensitivity, high interference resistance and affinity constant of 13.28 nM, is suitable for rapid monitoring of infant food, and reduces the economic cost by at least 40%.

The invention discloses a functionalized targeting fluorescent nanoprobe and application thereof in protein mutation analysis, and relates to the technical field of biology, the fluorescent nanoprobe comprises a targeting molecule-PEG hydrophilic bridging molecule and a matrix; the preparation method comprises the following steps: adding nano-seeds into fluorescent molecules to prepare fluorescent nano-particles, and stirring and mixing the fluorescent nano-particles with targeting molecules-PEG hydrophilic bridging molecules; the method is combined with deep learning to be applied to protein mutation analysis. The multifunctional integrated protein mutation receptor targeted fluorescent nanoprobe provided by the invention can accurately mark the position of a mutant protein at a single cell level, and can realize in-situ, real-time and dynamic living cell imaging of a tumor cell endogenous molecular mutant protein; and qualitative and quantitative analysis of tumor cell protein mutation can be realized through subcellular localization of the targeted fluorescent nanoprobe.

The invention belongs to the technical field of coal mine safety monitoring, and discloses a goaf coal spontaneous combustion hidden fire source fluorescent nanoparticle dynamic real-time temperature sensing detection method, which is based on the temperature-sensitive fluorescence characteristic of fluorescent nanoparticles, and comprises the following steps of: distributing a detection medium loaded with the fluorescent nanoparticles to a goaf; a fluorescence signal acquisition device is used for receiving fluorescence response signals generated by fluorescent nanoparticles under the influence of temperature in real time, and a signal analysis system is combined for processing and analyzing characteristic parameters of the fluorescence signals, so that dynamic real-time detection of the position, the temperature and the development trend of a coal spontaneous combustion hidden fire source in a goaf is realized.

The present application relates to fluorescent nanoparticles based on fluorinated zwitterionic random copolymer and its preparation method and application, the fluorescent nanoparticles include the single chain nanoparticles formed by poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine, the molecular chain end of the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine is connected with ACQ type group selected from anthryl, perylene imide group and pyrene base or AIE type group selected from tetraphenyl ethylene base, carbazole base and cyanine-containing triphenyl ethylene group.The fluorescent nanoparticles based on the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine of the present application that terminal fluorescent group is terminated, has excellent controllable fluorescent luminescence performance.It is considered that the single chain nanoparticles have isolation effect, avoid the fluorescence quenching effect of ACQ type group, and can provide limited microenvironment for AIE type group, ensure the fluorescent luminescence performance of nanoparticles.

The present invention provides compositions and methods for detecting tumor tissues in a subject. Embodiments of the present invention provide biocompatible chitosan nanoparticles suitable for dual modality optical imaging. Embodiments of the present invention provide methods for fabricating chitosan nanoparticles. Embodiments of the present invention provide methods for detecting tumor tissues in a subject using compositions provided by the present invention.

This invention discloses a fluorescent composite nanomaterial, its preparation method, and its application, belonging to the field of fluorescent materials technology. The fluorescent composite nanomaterial provided by this invention comprises carbon quantum dots with carboxyl groups on their surface and fluorescent nanoparticles with amino groups on their surface, in a mass ratio of (0.8–3):1; the carbon quantum dots with carboxyl groups and the fluorescent nanoparticles with amino groups are connected by amide bonds formed by the dehydration condensation of carboxyl and amino groups; the molecular formula of the fluorescent nanoparticles is Cs₂NaYCl₆:Yb. 3+ / Eu 3+ Among them, Yb 3+ The molar concentration of doping is 15–25%, Eu 3+ The molar concentration of the dopant is 8-12%. The fluorescent composite nanomaterials provided by this invention have the advantages of uniform particle size, high fluorescence emission intensity, and good fluorescence stability, and can be applied in fields such as humidity detection and fluorescence imaging.

The invention discloses a non-physical isolation digital ELISA detection method based on a fluorescent microsphere probe, a kit and application, and belongs to the technical field of biomedical detection. The method depends on a high-brightness fluorescent microsphere probe, realizes single-molecule immune recognition visualization and fluorescent particle digital counting under the condition of no physical isolation structure, simplifies system configuration based on an open type flat immune chip, improves the performance of a detection platform, overcomes the limitation of physical isolation type digital ELISA, is highly compatible with common equipment, and is suitable for large-scale popularization and application. The adaptation and popularization cost is reduced, and the portability and adaptability are good. High-brightness silicon-based fluorescent microspheres doped with fluorescent nanoparticles are adopted, so that high-sensitivity detection is realized; and establishing a database by using a standard product to realize absolute quantitative analysis. The kit takes a fluorescent silicon microsphere probe as a carrier, target protein can be accurately captured, non-specific adsorption is reduced, the function division of an open type flat chip is clear, and the operation is simple and convenient; the traditional detection sensitivity limitation can be broken through, the low-abundance protein is accurately quantified, the background noise interference is reduced, the detection specificity and accuracy are improved, and the early diagnosis of diseases is assisted.

The application discloses a preparation method of dendritic helical poly (phenylene ethynylene) and chiral fluorescent nanoparticles. The cinnamate is introduced into the dendritic alkyl ether element core point of the side group of poly (phenylene ethynylene) through molecular design, the synthesis steps are simple, the reaction is rapid, the prepared dendritic helical poly (phenylene ethynylene) can control the helical conformation and chiral circularly polarized luminescence properties through solvation, in addition, the dendritic helical poly (phenylene ethynylene) can be self-assembled into various chiral fluorescent nanoparticles, and cross-linking and curing are initiated by ultraviolet irradiation, so that the structure and chirality of the nanoparticles have good stability. The above-mentioned chiral fluorescent nanomaterial has high fluorescence quantum yield, good light and thermal stability, and has good application prospect in the field of chiral optoelectronic materials.

The present invention provides fluorescent nanoparticles with a long fluorescence lifetime and a method for producing the same. [Solution] The fluorescent silica nanoparticles of the present invention comprise silica, and rare earth fluorescent complexes and gold nanoparticles encapsulated within the silica. Furthermore, the method of the present invention for producing fluorescent silica nanoparticles includes providing gold nanoparticle-encapsulated silica nanoparticles containing gold nanoparticles, and coating the gold nanoparticle-encapsulated silica nanoparticles with silica containing a rare earth fluorescent complex.

According to the quantum dot perovskite photovoltaic system driven by the edge AI, a series photovoltaic panel sequentially comprises a silicon sub-cell layer, a perovskite absorption layer and a quantum dot coating from bottom to top, and an edge AI module is integrated in a frame area or a backboard area of the series photovoltaic panel and electrically connected with a plurality of sensors through data lines. The edge AI module is further connected with at least one actuator through a control line and used for adjusting physical parameters of the series photovoltaic panel, and the quantum dot coating is made of a lead-free carbon-based material, is 10-50 nm in thickness and covers the perovskite absorption layer. A three-junction series structure is adopted, unification of wide spectrum, high efficiency and high stability is achieved, the quantum dots are carbon-based fluorescent nanoparticles with the particle size range of 1-10 nm and grow on the surface of perovskite in situ through a chemical vapor deposition process, interface cleanliness and firm combination are ensured, use of toxic elements such as lead, cadmium and selenium is thoroughly avoided, and the quantum dots are prepared into the perovskite solar cell. And the environmental friendliness and the long-term operation reliability of the device are improved.

The invention belongs to the field of polypropylene materials, and relates to a composite rare earth element lossless industrial flaw detection polypropylene master batch and a preparation method thereof. According to the invention, through core-shell structure modification and surface grafting, the compatibility of lanthanide fluorescent nanoparticles and a polypropylene body is increased, so that the nanoparticles can be uniformly dispersed in a polypropylene matrix, and the overall performance of the composite material is improved. The characteristic that the composite rare earth element nanoparticles have an X-ray imaging effect is utilized, the composite rare earth element nanoparticles are compounded with the polypropylene material, industrial nondestructive inspection after the modified polypropylene material is processed into the automobile parts is achieved, and the problem that application of a conventional X-ray inspection means in the field of industrial inspection of the PP modified automobile parts is limited is solved.

The application discloses a preparation method of polymer fluorescent nanoparticles. First, a polylactic acid-polyurethane-polyacrylic acid copolymer is synthesized by reacting alcoholysis-recovered double-hydroxyl polylactic acid, 4,4'-diphenyl methane diisocyanate, polycaprolactone polyol, hydroxyethyl acrylate and acrylic acid; second, a polyethylene imine aqueous solution is prepared, and the polyethylene imine aqueous solution is added dropwise into a butyl acetate solution of the polylactic acid-polyurethane-polyacrylic acid copolymer, high-speed stirring is conducted, and a water-in-oil emulsion is formed; finally, the water-in-oil emulsion is heated, water is evaporated, a fluorescent solution is formed, and the fluorescent solution is subjected to spray drying to obtain the fluorescent nanoparticles. The nanoparticles of the paper cup are uniform in size, have excellent fluorescent performance and biocompatibility, and have a wide application prospect.

This invention provides a visualized medical hydrogel, its preparation method, and its application, belonging to the fields of biomedical materials and biomedical engineering technology. The preparation method is as follows: 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide are added to a gum arabic solution for activation, followed by the addition of 3-aminophenylborate salt. After the reaction is complete, the product is freeze-dried to obtain borate-treated gum arabic. Fluorescent nanoparticles are dissolved in water, and gelatin, borate-treated gum arabic, and arginine solution are added. 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide are then added for activation, yielding the visualized medical hydrogel. This visualized medical hydrogel exhibits excellent fluorescence, anti-inflammatory, and self-repairing functions, demonstrating good spinal cord injury repair performance and real-time monitoring performance in the application of biological tissue materials for spinal cord injury repair.

A method for ratiometric detection of putrescine using PtPd NPs@TPE fluorescent nanozymes. This invention first utilizes mesoporous platinum-palladium nanoparticles (PtPd NPs) doped with tetraphenylethylene (TPE) to obtain fluorescent nanoparticles (PtPd NPs@TPE) with oxidase-like activity, which can produce blue fluorescence under 365 nm ultraviolet light excitation. Secondly, this invention utilizes the antioxidant properties of putrescine solution to inhibit the formation of 2,3-diaminophenazine (DAP), an oxidation product, during the oxidation of o-phenylenediamine (OPD) by PtPd NPs@TPE, thereby reducing the DAP content. Based on the relationship between putrescine concentration and the colorimetric intensity of DAP, and the ratio of the yellow fluorescence intensity of DAP at 552 nm to the blue fluorescence intensity of PtPd NPs@TPE at 442 nm, a novel ratiometric fluorescence method for detecting putrescine is constructed. This method can be used to detect actual samples such as dried bean curd, with a detection limit as low as 0.0025 μg / mL.

The invention discloses a method for detecting breakage of non-metal cellosilk in a concrete structure, belongs to the field of nondestructive testing, and comprises a method for mixing microcapsules in a combined colloid and a method for detecting leakage of microcapsule wrappage. Wherein the microcapsule doping in the combined colloid refers to that three microcapsules which respectively wrap fluorescent nanoparticles, conductive graphene nanoplatelets and liquid sulfur hexafluoride are doped into an adhesive during fiber arrangement, and when the fibers are broken, microcapsule wrappings leak out, so that the fluorescent nanoparticles, the conductive graphene nanoplatelets and the liquid sulfur hexafluoride are uniformly distributed. And the broken wire position is determined through unmanned aerial vehicle inspection, electric signal detection and a gas detection method. The method for detecting the broken fibers in the concrete structure is high in measurement accuracy and good in feasibility, and the engineering problem that broken non-metal fibers in concrete are difficult to detect is solved.

This invention provides fluorescent nanoparticles and their conjugates, as well as methods for using them in in vivo and in vitro diagnostics and other applications. In some embodiments, the invention provides fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, the invention provides methods for manufacturing such nanoparticles. The nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AIEgen. TM Bright green.

Provided are fluorescent nanoparticles and their conjugates and methods of using the same for in vivo and in vitro diagnostics and other applications. In some embodiments, provided are fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, provided are methods of manufacturing such nanoparticles. Nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AIEgen™ Bright Green.

The application is a preparation method of a photoluminescence, reinforced, heat preservation and heat insulation nano composite coating. The method uses triethoxysilane as raw material, and prepares multi-level structure fluorescent nanoparticles with defect centers (=Si(O2), =Si:) through a mild (about 70 DEG C) sol-gel method, and then the nanoparticles are compounded into a coating matrix, and a solid and durable fluorescent coating is developed by using the defect-induced luminescence technology; in addition, the multi-level cavity structure of the nanoparticles can encapsulate more air, so as to provide excellent heat preservation and heat insulation performance for the coating. The obtained nano composite coating with photoluminescence, excellent mechanical performance and heat preservation and heat insulation expands the commercial application field of the fluorescent coating.

The disclosure provides marine polysaccharide vector-based anthocyanin nanoparticles, using marine polysaccharides, food-borne fluorescent nanoparticles and sodium hyaluronate as a composite vector to be combined with anthocyanin, and is prepared by a method: S1, dissolving sodium alginate in phosphate buffered saline, and successively adding EDC, HOBt and the food-borne fluorescent nanoparticles to obtain a fluorescence-alginate complex; S2, adding the fluorescence-alginate complex into a mixture of DMF and DCM, and then adding DIC and DMAP; S3, adding the anthocyanin and hyaluronic acid, and then removing a solvent; and S4, removing unreacted small molecules, and carrying out freeze drying.

The invention discloses a ratio type fluorescent nanometer thermometer based on temperature response type amphiphilic macromolecules and preparation and application of the ratio type fluorescent nanometer thermometer, and belongs to the technical field of fluorescent temperature detection and biomedical detection.The temperature probe is composed of two types of fluorescent nanoparticles with opposite temperature response behaviors, the nanoparticles are formed by self-assembly of temperature-sensitive amphiphilic macromolecules containing aggregation-induced emission molecules, fluorescence of one type of nanoparticles is enhanced due to aggregation of fluorescent molecules induced by collapse of temperature-sensitive polymer chains when the temperature rises, and fluorescence of the other type of nanoparticles is weakened due to de-aggregation or spatial migration of the fluorescent molecules when the temperature rises. Temperature detection is realized by monitoring the change of the intensity ratio of the two fluorescence signals; the temperature probe has high temperature sensitivity and good thermal resolution in a physiological temperature range, and can be used for real-time monitoring of temperature change in living cells.

The invention relates to the technical field of label production, in particular to a production process of a substrate-free label material, which comprises the following steps: carrying out plasma surface treatment on PET release paper to improve the surface performance; dimethicone is diluted, the surface of PET release paper is coated with the diluted dimethicone through the micro-concave coating technology, and then the coated PET release paper is cured to form a silicone oil layer; preparing an acrylic acid modified pressure-sensitive adhesive containing anti-counterfeiting elements, coating the acrylic acid modified pressure-sensitive adhesive on the surface of the silicone oil layer, and pre-curing to form an anti-counterfeiting pressure-sensitive adhesive layer; printing functional ink containing fluorescent nanoparticles on the surface of the pressure-sensitive adhesive layer by using ink-jet printing equipment to form a patterned anti-counterfeiting layer; the interface bonding force of the ink-jet layer and the pressure-sensitive adhesive reaches 2.5 N / cm, no residue is left after stripping, and the fluorescent nanoparticles are clearly developed under ultraviolet light.

The invention relates to the technical field of ultrafast preparation of fluorescent nanoparticles, and discloses a fluorescent nanoparticle as well as a preparation method and application thereof. The preparation method comprises the following steps: mechanically emulsifying a mixed solution of an oil phase and a water phase to obtain an oil-in-water emulsion, and reacting under a microwave condition to obtain the fluorescent nanoparticles, wherein the oil phase comprises an initiator, an optional functional monomer and a fluorescent monomer; the water phase comprises an emulsifier, inorganic salt and water. According to the method provided by the invention, different types of small-size fluorescent nanoparticles are prepared in an ultra-fast manner under the assistance of microwaves, and the fluorescent nanoparticles which are uniform in particle size, have the hydration particle size of 50-900 nm and have different modification groups can be prepared; the fluorescent probe has the advantages of being short in reaction time, high in product functionality and excellent in fluorescence intensity performance, AIE and ACQ luminescence is achieved at the same time, the preparation process is simple, green and efficient, and the fluorescent probe can be applied to the fields of fluorescence living imaging, multi-mode imaging, drug delivery, treatment monitoring and the like.

The invention belongs to the technical crossing field of semiconductors and biological nanomaterials, and particularly relates to biocompatible perovskite fluorescent nanoparticles and a preparation method thereof.The preparation method comprises the steps that brominated acid and oleylamine are mixed in a solvent to prepare a zwitterionic ligand solution, and a Pb source and an HBr solution are mixed to prepare a premixed solution; mixing and stirring the premixed solution, CsAc and the zwitterionic ligand solution, and then carrying out solid-liquid separation to obtain the aqueous-phase perovskite quantum dots, the preparation method comprises the following steps: dissolving water-phase perovskite quantum dots and distearoyl phosphatidyl ethanolamine-polyethylene glycol in an organic solvent, and then heating and evaporating to obtain a lipid dry film; immersing the dry lipid membrane in pure water, and carrying out deep mixing treatment to obtain a liposome emulsion; and extruding the liposome emulsion through a micron-sized filter membrane to obtain the perovskite fluorescent nanoparticles. The prepared perovskite fluorescent nanoparticles have good water stability and biocompatibility and are suitable for biological cell imaging, a large dose of toxic chemical reagents and / or inert reaction environment conditions are not needed in the preparation process, and the problem that commercialization of water stability and biocompatibility of the perovskite fluorescent nanoparticles is difficult to achieve is solved.

A library of barcoded particle population including combinations of inorganic fluorescent nanoparticles leading to unique identification of the barcoded particle population, and an essay kit including the library of barcoded particle populations and at least one fluorescent protein. Also, a device for biological assay configured to separate at an individual level the composite particles from the assay kit, dispersed in a biological sample and including a reader having at least one illumination source; and a light detector measuring light intensity and coupled to a spectrometer, wherein the at least one illumination source triggers fluorescence of the inorganic fluorescent nanoparticles encapsulated in composite particles and fluorescence of the at least one fluorescent protein

Provided are fluorescent nanoparticles and their conjugates and methods of using the same for in vivo and in vitro diagnostics and other applications. In some embodiments, provided are fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, provided are methods of manufacturing such nanoparticles. Nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AlEgen™ Bright Green.

The invention discloses an invisible two-dimensional code and a preparation method and an identification method thereof, and belongs to the technical field of two-dimensional code anti-counterfeiting. The preparation method comprises the following steps: mixing rare earth doped inorganic fluorescent nanoparticles with the average particle size of 20-50nm and the doping concentration of 2%-4% (molar percentage) with a transparent resin matrix to prepare a solution; forming a two-dimensional code pattern on the substrate through a photoetching and stripping or ink-jet printing process; and finally, curing to form the invisible two-dimensional code film. Through innovative combination of materials and a process, the prepared invisible two-dimensional code is high in light transmittance and invisible under natural light, clear in display and high in recognition rate under excitation of ultraviolet or infrared light, has excellent adhesive force, wear resistance and environmental durability, is particularly suitable for being integrated below a display area of a display panel, and has a wide application prospect. And information hidden storage and high-efficiency anti-counterfeiting are realized while the attractiveness of the equipment is kept.

The invention provides an intelligent response type engine airtightness detection leakage test liquid and a preparation method thereof. The leakage testing liquid for air tightness detection is prepared from the following components in parts by weight: 88 to 92 parts of a component A, 6 to 8 parts of a component B, 1 to 3 parts of a component C and 0.5 to 1.5 parts of a component D, wherein the component A comprises deionized water, xanthan gum, konjac glucomannan and a 5% poly (N-isopropylacrylamide) aqueous solution, and is used as a bionic self-repairing solvent; the component B comprises a dopamine-phytic acid composite solution and a beta-cyclodextrin loaded benzotriazole nanocapsule dispersion liquid with the solid content of 10%, and is used as an anti-rust system; the component C comprises locust bean gum, soyasaponin and pH response type porphyrin derivative fluorescent nanoparticle dispersion liquid and serves as a bionic foaming and tracing system, and the component D comprises molybdenum disulfide nanosheet dispersion liquid and serves as an auxiliary functional additive. Aiming at the problems in the prior art, the invention designs the novel engine air tightness detection leakage testing liquid which can simultaneously take the self-repairing performance, the anti-rust performance and the tracing precision into account.

The invention discloses a method for improving the stability of rare earth fluorescent nanoparticles, and relates to the technical field of nanomaterials, the method comprises the following steps: coupling rare earth fluorescent nanoparticles with carboxyl groups on the surfaces with streptavidin to obtain a rare earth fluorescent nanoparticle-streptavidin conjugate with biotin recognition sites; then tetrahedral DNA framework nucleic acid with the tail end modified with biotin is specifically combined with the rare earth fluorescent nanoparticle-streptavidin conjugate through biotin-streptavidin, so that the tetrahedral DNA framework nucleic acid is modified on the surface of the rare earth fluorescent nanoparticle, the functionality of the rare earth fluorescent nanoparticle is kept, and meanwhile, the rare earth fluorescent nanoparticle-streptavidin conjugate is obtained. The dispersity and the storage stability of the water phase system are effectively improved.