61 results about "Fluorescent nanoparticles" patented technology

The invention discloses a fluorescent composite nano material as well as a preparation method and application thereof, and belongs to the technical field of fluorescent materials. The fluorescent composite nano-material provided by the invention comprises carbon quantum dots with carboxyl modified surfaces and fluorescent nano-particles with amino modified surfaces, wherein the mass ratio of the carbon quantum dots to the fluorescent nano-particles is (0.8-3): 1; the carbon quantum dots of which the surfaces are modified with carboxyl groups are connected with the fluorescent nanoparticles of which the surfaces are modified with amino groups through amido bonds formed by dehydration condensation of carboxyl groups and amino groups; the molecular formula of the fluorescent nanoparticles is Cs2NaYCl6: Yb < 3 + > / Eu < 3 + >, the molar concentration of doped Yb < 3 + > is 15-25%, and the molar concentration of doped Eu < 3 + > is 8-12%. The fluorescent composite nanomaterial provided by the invention has the advantages of uniform particle size and high fluorescence emission intensity, has good fluorescence stability, and can be applied to the fields of humidity detection, fluorescence imaging and the like.

The invention discloses a high-resolution semiconductor surface and interior flaw detection method and device based on a multi-photon nonlinear fluorescence effect, and relates to nonlinear optics and optical imaging semiconductor detection. The short-wave infrared light penetrates through a semiconductor to be detected, a surface or an internal defect to trigger scattering, the transmittance / reflectivity and the power are changed, the thin film containing the high-order nonlinear fluorescent nanoparticles is excited, the luminance generates magnitude order change, and the defect detection contrast ratio and the imaging resolution ratio are improved. Full-automatic control is achieved through an FPGA and LabVIEW, sub-images are automatically collected and spliced, and gray scale abnormal areas are analyzed to judge defect types and distribution. The optical imaging device comprises an exciting light generation module, a sample scanning module, a nonlinear fluorescent film imaging module and an intelligent control analysis module, realizes short-wave infrared light excitation and visible light-near infrared detection, and has the advantages of intelligence, high resolution, low cost and large-area full-automatic flaw detection. The method is suitable for large-scale semiconductor efficient flaw detection, gets rid of dependence on an infrared imaging camera, and reduces system cost.

The present disclosure relates to an azide-modified fluorescent π-conjugated polymer and a DNA-modified π-conjugated fluorescent nanoparticle-based protein detection imaging method capable of signal rapid amplification and high throughput. The present disclosure prepares oligonucleotide-modified fluorescent π-conjugated polymers (OCPNs) based on the azide-modified fluorescent π-conjugated polymer. The OCPNs have ultrahigh fluorescence luminance and extremely high detection sensitivity, and can complete detection and imaging of targets in different abundances without the need of an additional fluorescence signal amplification step. In addition, a rapid and specific targeting effect can be achieved with an extremely small dosage and an entire staining and imaging process can be shortened, so that the OCPNs achieve an extremely high imaging efficiency during multi-color imaging and thus exhibit great application prospects.

The invention discloses a double-ligand rare earth fluorescent nanoparticle for sensitively detecting copper ions, the double-ligand rare earth fluorescent nanoparticle is a lanthanide element-based ratio type fluorescent nanoparticle for detecting Cu < 2 + >, and the fluorescent nanoparticle has double emission sites, the fluorescent nano particles show strong blue and red fluorescence at the positions of 423 nm and 618 nm; the preparation method of the double-ligand rare earth fluorescent nanoparticles comprises the following steps: preparing a europium nitrate solution, adding a DMSO solution containing luminol into the europium nitrate solution, then adding a PBS buffer solution into the europium nitrate solution, and stirring at room temperature to obtain a clear and transparent solution containing a precursor Eu-lu complex; the preparation method of the double-ligand rare earth fluorescent nanoparticles is simple, the copper ions can be stably measured for a long time, the stability is good, the preservation time is long, and the double-ligand rare earth fluorescent nanoparticles play an important role in high-sensitivity and visual detection of the copper ions.

The application provides a fluorescent sensor based on a tripod [5] arene, which is obtained by self-assembly complexation of a host of a tripod [5] arene TP5 and a guest of a quaternary ammonium salt in an H2O-DMSO system. A supramolecular system is constructed by host-guest interaction between the tripod [5] arene TP5 and the amphiphilic guest TPN, and then PbS fluorescent nanoparticles are generated in situ under ion induction. Fluorescence enhancement and high-sensitivity detection (LOD=7.00*10 2+ -6 M) of Pb ‑8 are realized. In addition, the fluorescent supramolecular polymer network based on the PbS fluorescent nanoparticles can be successfully applied to in-situ detection of Pb 2+ in cells.

The preparation method comprises the following steps: firstly, dissolving gelatin in deionized water to form a gelatin solution, adding rhodamine B and a catalyst, and carrying out amidation reaction to chemically bond amino groups of the gelatin with carboxyl groups of the rhodamine B; then adding a cross-linking agent to cross-link the rhodamine B-gelatin conjugate into nano particles, and enabling the rhodamine B-gelatin fluorescent nano thermometer to be ellipsoidal by utilizing centrifugal force formed by high-speed stirring in the cross-linking process. The rhodamine B is firmly loaded on the gelatin nanoparticles, the loading capacity of the rhodamine B is greatly increased, the residence time of the rhodamine B in an organism is prolonged, the photobleaching effect of the rhodamine B is overcome, the rhodamine B-gelatin fluorescent nanoparticles are prepared into ellipsoids through uneven stress under the action of centrifugal force when aggregated particles are formed, and the rhodamine B-gelatin fluorescent nanoparticles are prepared into the ellipsoids. The preparation method is simple, the temperature measurement sensitivity is high, the photobleaching phenomenon of rhodamine B is effectively weakened, and the biomedical utilization prospect is good.

The invention relates to the technical field of biology, and particularly discloses a cronobacter sakazakii nucleic acid aptamer and application thereof in preparation of a biosensor. According to the invention, a nucleic acid aptamer with a DNA sequence as shown in SEQ ID No: 1 is designed for Cronobacter sakazakii MipA protein, and 5'terminal amino is modified. The aptamer can be used for constructing a fluorescence quenching type biosensor, and comprises a fluorescent probe (fluorescent nanoparticles synthesized by citric acid diammonium, thiourea, tetramethyl orthosilicate and cerium dioxide, and a first aptamer modified by carboxyl) and a quenching probe (citric acid reduced gold nanoparticles, a second aptamer modified by sulfydryl, SEQ ID No: 2). Compared with a traditional method, the screening efficiency is remarkably improved, and the detection time is lt; the method has the advantages of high sensitivity, high interference resistance and affinity constant of 13.28 nM, is suitable for rapid monitoring of infant food, and reduces the economic cost by at least 40%.

The invention discloses a functionalized targeting fluorescent nanoprobe and application thereof in protein mutation analysis, and relates to the technical field of biology, the fluorescent nanoprobe comprises a targeting molecule-PEG hydrophilic bridging molecule and a matrix; the preparation method comprises the following steps: adding nano-seeds into fluorescent molecules to prepare fluorescent nano-particles, and stirring and mixing the fluorescent nano-particles with targeting molecules-PEG hydrophilic bridging molecules; the method is combined with deep learning to be applied to protein mutation analysis. The multifunctional integrated protein mutation receptor targeted fluorescent nanoprobe provided by the invention can accurately mark the position of a mutant protein at a single cell level, and can realize in-situ, real-time and dynamic living cell imaging of a tumor cell endogenous molecular mutant protein; and qualitative and quantitative analysis of tumor cell protein mutation can be realized through subcellular localization of the targeted fluorescent nanoprobe.

The invention belongs to the technical field of coal mine safety monitoring, and discloses a goaf coal spontaneous combustion hidden fire source fluorescent nanoparticle dynamic real-time temperature sensing detection method, which is based on the temperature-sensitive fluorescence characteristic of fluorescent nanoparticles, and comprises the following steps of: distributing a detection medium loaded with the fluorescent nanoparticles to a goaf; a fluorescence signal acquisition device is used for receiving fluorescence response signals generated by fluorescent nanoparticles under the influence of temperature in real time, and a signal analysis system is combined for processing and analyzing characteristic parameters of the fluorescence signals, so that dynamic real-time detection of the position, the temperature and the development trend of a coal spontaneous combustion hidden fire source in a goaf is realized.

The present application relates to fluorescent nanoparticles based on fluorinated zwitterionic random copolymer and its preparation method and application, the fluorescent nanoparticles include the single chain nanoparticles formed by poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine, the molecular chain end of the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine is connected with ACQ type group selected from anthryl, perylene imide group and pyrene base or AIE type group selected from tetraphenyl ethylene base, carbazole base and cyanine-containing triphenyl ethylene group.The fluorescent nanoparticles based on the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine of the present application that terminal fluorescent group is terminated, has excellent controllable fluorescent luminescence performance.It is considered that the single chain nanoparticles have isolation effect, avoid the fluorescence quenching effect of ACQ type group, and can provide limited microenvironment for AIE type group, ensure the fluorescent luminescence performance of nanoparticles.

The present invention relates to a fluorescent nanoparticle, a fluorescent probe, a preparation method and an application thereof. The fluorescent nanoparticle is an SrS nanoparticle doped with Pr, Sm and Mn. The fluorescent probe comprises mesoporous silica loaded with the fluorescent nanoparticle, a DNAzyme complex coupled to the surface of the mesoporous silica, and a shell located on the outer layer of the DNAzyme complex. In the presence of near-infrared light and a specific nucleic acid fragment, the present invention activates the fluorescent probe system and releases the cyanine dye FD-1080 in the DNAzyme complex structure, and monitors the near-infrared second-region fluorescence emission of FD-1080 to realize the analysis of the target fragment. The present invention not only broadens the selectable fluorescent nanomaterials for biological imaging, but also contributes to the development of near-infrared light-controlled biochemical sensors and near-infrared light-controlled drug delivery carriers, and promotes the application of fluorescence analysis technology and light-controlled nanomachines in the field of biomedical detection.

The present invention provides fluorescent nanoparticles comprising a zwitterionic complex comprising a cationically cross-linked polyethyleneimine and an anionic polymer comprising one or more repeating units represented by a moiety of Formula 1 wherein R1 is hydrogen or a C1-C3 alkyl group; and R2 is-CO2-or-CO2 (CH2) mCO2-, wherein m is an integer selected from 1 to 4. # imgabs0 #

The present invention provides compositions and methods for detecting tumor tissues in a subject. Embodiments of the present invention provide biocompatible chitosan nanoparticles suitable for dual modality optical imaging. Embodiments of the present invention provide methods for fabricating chitosan nanoparticles. Embodiments of the present invention provide methods for detecting tumor tissues in a subject using compositions provided by the present invention.

This invention discloses a fluorescent composite nanomaterial, its preparation method, and its application, belonging to the field of fluorescent materials technology. The fluorescent composite nanomaterial provided by this invention comprises carbon quantum dots with carboxyl groups on their surface and fluorescent nanoparticles with amino groups on their surface, in a mass ratio of (0.8–3):1; the carbon quantum dots with carboxyl groups and the fluorescent nanoparticles with amino groups are connected by amide bonds formed by the dehydration condensation of carboxyl and amino groups; the molecular formula of the fluorescent nanoparticles is Cs₂NaYCl₆:Yb. 3+ / Eu 3+ Among them, Yb 3+ The molar concentration of doping is 15–25%, Eu 3+ The molar concentration of the dopant is 8-12%. The fluorescent composite nanomaterials provided by this invention have the advantages of uniform particle size, high fluorescence emission intensity, and good fluorescence stability, and can be applied in fields such as humidity detection and fluorescence imaging.

The invention discloses a non-physical isolation digital ELISA detection method based on a fluorescent microsphere probe, a kit and application, and belongs to the technical field of biomedical detection. The method depends on a high-brightness fluorescent microsphere probe, realizes single-molecule immune recognition visualization and fluorescent particle digital counting under the condition of no physical isolation structure, simplifies system configuration based on an open type flat immune chip, improves the performance of a detection platform, overcomes the limitation of physical isolation type digital ELISA, is highly compatible with common equipment, and is suitable for large-scale popularization and application. The adaptation and popularization cost is reduced, and the portability and adaptability are good. High-brightness silicon-based fluorescent microspheres doped with fluorescent nanoparticles are adopted, so that high-sensitivity detection is realized; and establishing a database by using a standard product to realize absolute quantitative analysis. The kit takes a fluorescent silicon microsphere probe as a carrier, target protein can be accurately captured, non-specific adsorption is reduced, the function division of an open type flat chip is clear, and the operation is simple and convenient; the traditional detection sensitivity limitation can be broken through, the low-abundance protein is accurately quantified, the background noise interference is reduced, the detection specificity and accuracy are improved, and the early diagnosis of diseases is assisted.

The invention belongs to the technical field of biomedical materials and biomedical engineering, and discloses a selenium nanoparticle fluorescent probe capable of imaging in vivo as well as a preparation method and application of the selenium nanoparticle fluorescent probe. The preparation method comprises the following steps: carrying out hydrothermal reaction on a mixed solution of sodium selenite pentahydrate, citric acid and arginine to generate a selenium quantum dot (SeQDs) solution; dialyzing the selenium quantum dot solution, and freeze-drying to obtain the pure selenium quantum dot. Selenium quantum dots (SeQDs) are grafted to indocyanine green (ICG), and a selenium quantum dot indocyanine green compound (SeQDs (at) ICG) is obtained. The fluorescent nanoparticles prepared by the preparation method have excellent tissue penetrating power, fluorescence performance and oxidation resistance, and have good spinal cord injury repair performance and injury repair real-time monitoring performance when applied to spinal cord injury repair biological tissue materials.

The application discloses a preparation method of dendritic helical poly (phenylene ethynylene) and chiral fluorescent nanoparticles. The cinnamate is introduced into the dendritic alkyl ether element core point of the side group of poly (phenylene ethynylene) through molecular design, the synthesis steps are simple, the reaction is rapid, the prepared dendritic helical poly (phenylene ethynylene) can control the helical conformation and chiral circularly polarized luminescence properties through solvation, in addition, the dendritic helical poly (phenylene ethynylene) can be self-assembled into various chiral fluorescent nanoparticles, and cross-linking and curing are initiated by ultraviolet irradiation, so that the structure and chirality of the nanoparticles have good stability. The above-mentioned chiral fluorescent nanomaterial has high fluorescence quantum yield, good light and thermal stability, and has good application prospect in the field of chiral optoelectronic materials.

The present invention provides fluorescent nanoparticles with a long fluorescence lifetime and a method for producing the same. [Solution] The fluorescent silica nanoparticles of the present invention comprise silica, and rare earth fluorescent complexes and gold nanoparticles encapsulated within the silica. Furthermore, the method of the present invention for producing fluorescent silica nanoparticles includes providing gold nanoparticle-encapsulated silica nanoparticles containing gold nanoparticles, and coating the gold nanoparticle-encapsulated silica nanoparticles with silica containing a rare earth fluorescent complex.

The invention belongs to the field of food safety detection, and particularly discloses a fluorescent paper chip for detecting Escherichia coli O157: H7 and a preparation method and application thereof.Escherichia coli serves as a research object, molecularly imprinted fluorescent nanoparticles with Escherichia coli O157: H7 as a template are designed and synthesized through a molecular imprinting technology, and the fluorescent paper chip is used for detecting Escherichia coli O157: H7. After the surface of the paper chip is modified with the molecularly imprinted fluorescent nanoparticles, an Escherichia coli template can be sensitively and quickly recognized according to the change of fluorescence quenching rate, so that the paper-based array chip capable of specifically recognizing Escherichia coli O157: H7 is prepared, and the paper-based array chip has the characteristics of simplicity, low cost, real-time property and the like.

According to the quantum dot perovskite photovoltaic system driven by the edge AI, a series photovoltaic panel sequentially comprises a silicon sub-cell layer, a perovskite absorption layer and a quantum dot coating from bottom to top, and an edge AI module is integrated in a frame area or a backboard area of the series photovoltaic panel and electrically connected with a plurality of sensors through data lines. The edge AI module is further connected with at least one actuator through a control line and used for adjusting physical parameters of the series photovoltaic panel, and the quantum dot coating is made of a lead-free carbon-based material, is 10-50 nm in thickness and covers the perovskite absorption layer. A three-junction series structure is adopted, unification of wide spectrum, high efficiency and high stability is achieved, the quantum dots are carbon-based fluorescent nanoparticles with the particle size range of 1-10 nm and grow on the surface of perovskite in situ through a chemical vapor deposition process, interface cleanliness and firm combination are ensured, use of toxic elements such as lead, cadmium and selenium is thoroughly avoided, and the quantum dots are prepared into the perovskite solar cell. And the environmental friendliness and the long-term operation reliability of the device are improved.

The invention belongs to the field of polypropylene materials, and relates to a composite rare earth element lossless industrial flaw detection polypropylene master batch and a preparation method thereof. According to the invention, through core-shell structure modification and surface grafting, the compatibility of lanthanide fluorescent nanoparticles and a polypropylene body is increased, so that the nanoparticles can be uniformly dispersed in a polypropylene matrix, and the overall performance of the composite material is improved. The characteristic that the composite rare earth element nanoparticles have an X-ray imaging effect is utilized, the composite rare earth element nanoparticles are compounded with the polypropylene material, industrial nondestructive inspection after the modified polypropylene material is processed into the automobile parts is achieved, and the problem that application of a conventional X-ray inspection means in the field of industrial inspection of the PP modified automobile parts is limited is solved.

The application discloses a preparation method of polymer fluorescent nanoparticles. First, a polylactic acid-polyurethane-polyacrylic acid copolymer is synthesized by reacting alcoholysis-recovered double-hydroxyl polylactic acid, 4,4'-diphenyl methane diisocyanate, polycaprolactone polyol, hydroxyethyl acrylate and acrylic acid; second, a polyethylene imine aqueous solution is prepared, and the polyethylene imine aqueous solution is added dropwise into a butyl acetate solution of the polylactic acid-polyurethane-polyacrylic acid copolymer, high-speed stirring is conducted, and a water-in-oil emulsion is formed; finally, the water-in-oil emulsion is heated, water is evaporated, a fluorescent solution is formed, and the fluorescent solution is subjected to spray drying to obtain the fluorescent nanoparticles. The nanoparticles of the paper cup are uniform in size, have excellent fluorescent performance and biocompatibility, and have a wide application prospect.

This invention provides a visualized medical hydrogel, its preparation method, and its application, belonging to the fields of biomedical materials and biomedical engineering technology. The preparation method is as follows: 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide are added to a gum arabic solution for activation, followed by the addition of 3-aminophenylborate salt. After the reaction is complete, the product is freeze-dried to obtain borate-treated gum arabic. Fluorescent nanoparticles are dissolved in water, and gelatin, borate-treated gum arabic, and arginine solution are added. 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide are then added for activation, yielding the visualized medical hydrogel. This visualized medical hydrogel exhibits excellent fluorescence, anti-inflammatory, and self-repairing functions, demonstrating good spinal cord injury repair performance and real-time monitoring performance in the application of biological tissue materials for spinal cord injury repair.

A method for ratiometric detection of putrescine using PtPd NPs@TPE fluorescent nanozymes. This invention first utilizes mesoporous platinum-palladium nanoparticles (PtPd NPs) doped with tetraphenylethylene (TPE) to obtain fluorescent nanoparticles (PtPd NPs@TPE) with oxidase-like activity, which can produce blue fluorescence under 365 nm ultraviolet light excitation. Secondly, this invention utilizes the antioxidant properties of putrescine solution to inhibit the formation of 2,3-diaminophenazine (DAP), an oxidation product, during the oxidation of o-phenylenediamine (OPD) by PtPd NPs@TPE, thereby reducing the DAP content. Based on the relationship between putrescine concentration and the colorimetric intensity of DAP, and the ratio of the yellow fluorescence intensity of DAP at 552 nm to the blue fluorescence intensity of PtPd NPs@TPE at 442 nm, a novel ratiometric fluorescence method for detecting putrescine is constructed. This method can be used to detect actual samples such as dried bean curd, with a detection limit as low as 0.0025 μg / mL.

The invention discloses a preparation method of fluorescent nanoparticles and the fluorescent nanoparticles prepared by the preparation method. Comprising the following steps: mixing a mixed solution containing a non-conjugated polymer, fluorescent molecules and a solvent I with a solvent II to obtain the fluorescent nanoparticles. The preparation method is simple, low in cost, short in period and high in yield. The method is not limited by the chemical structure of fluorescent molecules, and any type of fluorescent molecules can be used. The hydrogen bonds can be destroyed through estimation, so that the fluorescent molecules can be quickly released from the self-assembled nano-particles, and other complex operations are not needed.

The invention provides preparation and application of a double-macrocyclic molecule based on pillar [5] arene and a supramolecular system of the double-macrocyclic molecule. A double-macrocyclic host molecule DNPB based on pillar [5] arene is synthesized firstly, the double-macrocyclic host molecule DNPB comprises two macrocyclic cavities with different rigidity and electronic structures, the cavities can be assembled with different objects step by step according to respective structural characteristics, and a supramolecular functional system HG1G2 with richer performance is constructed. Due to steric hindrance and packaging effect provided by double-large-ring assembly, the free radical emission characteristic of HG1G2 is remarkably improved. Meanwhile, by utilizing the good photoluminescence and photochromic properties of the supramolecular assembly system HG1G2, the supramolecular assembly system HG1G2 is introduced into polymethyl methacrylate, so that the photochromic and photoluminescence processes are more stable in a solid system, and the supramolecular assembly system HG1G2 is applied to hierarchical information encryption. Besides, different metal ions are introduced into a host-guest assembly system by utilizing cavities of different dimensions of the column [5] arene double-macrocyclic DNPB through an orthogonal self-assembly method of host-guest interaction and metal coordination interaction to construct a series of in-situ limited supramolecular polymer-based fluorescent nanoparticles (FSNPs), and the in-situ limited supramolecular polymer-based fluorescent nanoparticles are applied to cell imaging.

The invention discloses a method for detecting breakage of non-metal cellosilk in a concrete structure, belongs to the field of nondestructive testing, and comprises a method for mixing microcapsules in a combined colloid and a method for detecting leakage of microcapsule wrappage. Wherein the microcapsule doping in the combined colloid refers to that three microcapsules which respectively wrap fluorescent nanoparticles, conductive graphene nanoplatelets and liquid sulfur hexafluoride are doped into an adhesive during fiber arrangement, and when the fibers are broken, microcapsule wrappings leak out, so that the fluorescent nanoparticles, the conductive graphene nanoplatelets and the liquid sulfur hexafluoride are uniformly distributed. And the broken wire position is determined through unmanned aerial vehicle inspection, electric signal detection and a gas detection method. The method for detecting the broken fibers in the concrete structure is high in measurement accuracy and good in feasibility, and the engineering problem that broken non-metal fibers in concrete are difficult to detect is solved.

This invention provides fluorescent nanoparticles and their conjugates, as well as methods for using them in in vivo and in vitro diagnostics and other applications. In some embodiments, the invention provides fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, the invention provides methods for manufacturing such nanoparticles. The nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AIEgen. TM Bright green.

Provided are fluorescent nanoparticles and their conjugates and methods of using the same for in vivo and in vitro diagnostics and other applications. In some embodiments, provided are fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, provided are methods of manufacturing such nanoparticles. Nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AIEgen™ Bright Green.

The application is a preparation method of a photoluminescence, reinforced, heat preservation and heat insulation nano composite coating. The method uses triethoxysilane as raw material, and prepares multi-level structure fluorescent nanoparticles with defect centers (=Si(O2), =Si:) through a mild (about 70 DEG C) sol-gel method, and then the nanoparticles are compounded into a coating matrix, and a solid and durable fluorescent coating is developed by using the defect-induced luminescence technology; in addition, the multi-level cavity structure of the nanoparticles can encapsulate more air, so as to provide excellent heat preservation and heat insulation performance for the coating. The obtained nano composite coating with photoluminescence, excellent mechanical performance and heat preservation and heat insulation expands the commercial application field of the fluorescent coating.