18 results about "Fluorescent nanoparticles" patented technology

The invention belongs to the technical field of coal mine safety monitoring, and discloses a goaf coal spontaneous combustion hidden fire source fluorescent nanoparticle dynamic real-time temperature sensing detection method, which is based on the temperature-sensitive fluorescence characteristic of fluorescent nanoparticles, and comprises the following steps of: distributing a detection medium loaded with the fluorescent nanoparticles to a goaf; a fluorescence signal acquisition device is used for receiving fluorescence response signals generated by fluorescent nanoparticles under the influence of temperature in real time, and a signal analysis system is combined for processing and analyzing characteristic parameters of the fluorescence signals, so that dynamic real-time detection of the position, the temperature and the development trend of a coal spontaneous combustion hidden fire source in a goaf is realized.

The present application relates to fluorescent nanoparticles based on fluorinated zwitterionic random copolymer and its preparation method and application, the fluorescent nanoparticles include the single chain nanoparticles formed by poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine, the molecular chain end of the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine is connected with ACQ type group selected from anthryl, perylene imide group and pyrene base or AIE type group selected from tetraphenyl ethylene base, carbazole base and cyanine-containing triphenyl ethylene group.The fluorescent nanoparticles based on the poly trifluoroethyl methacrylate-co-methacryloyloxyethyl sulfobetaine of the present application that terminal fluorescent group is terminated, has excellent controllable fluorescent luminescence performance.It is considered that the single chain nanoparticles have isolation effect, avoid the fluorescence quenching effect of ACQ type group, and can provide limited microenvironment for AIE type group, ensure the fluorescent luminescence performance of nanoparticles.

The present invention provides compositions and methods for detecting tumor tissues in a subject. Embodiments of the present invention provide biocompatible chitosan nanoparticles suitable for dual modality optical imaging. Embodiments of the present invention provide methods for fabricating chitosan nanoparticles. Embodiments of the present invention provide methods for detecting tumor tissues in a subject using compositions provided by the present invention.

The present invention provides fluorescent nanoparticles with a long fluorescence lifetime and a method for producing the same. [Solution] The fluorescent silica nanoparticles of the present invention comprise silica, and rare earth fluorescent complexes and gold nanoparticles encapsulated within the silica. Furthermore, the method of the present invention for producing fluorescent silica nanoparticles includes providing gold nanoparticle-encapsulated silica nanoparticles containing gold nanoparticles, and coating the gold nanoparticle-encapsulated silica nanoparticles with silica containing a rare earth fluorescent complex.

According to the quantum dot perovskite photovoltaic system driven by the edge AI, a series photovoltaic panel sequentially comprises a silicon sub-cell layer, a perovskite absorption layer and a quantum dot coating from bottom to top, and an edge AI module is integrated in a frame area or a backboard area of the series photovoltaic panel and electrically connected with a plurality of sensors through data lines. The edge AI module is further connected with at least one actuator through a control line and used for adjusting physical parameters of the series photovoltaic panel, and the quantum dot coating is made of a lead-free carbon-based material, is 10-50 nm in thickness and covers the perovskite absorption layer. A three-junction series structure is adopted, unification of wide spectrum, high efficiency and high stability is achieved, the quantum dots are carbon-based fluorescent nanoparticles with the particle size range of 1-10 nm and grow on the surface of perovskite in situ through a chemical vapor deposition process, interface cleanliness and firm combination are ensured, use of toxic elements such as lead, cadmium and selenium is thoroughly avoided, and the quantum dots are prepared into the perovskite solar cell. And the environmental friendliness and the long-term operation reliability of the device are improved.

The application discloses a preparation method of polymer fluorescent nanoparticles. First, a polylactic acid-polyurethane-polyacrylic acid copolymer is synthesized by reacting alcoholysis-recovered double-hydroxyl polylactic acid, 4,4'-diphenyl methane diisocyanate, polycaprolactone polyol, hydroxyethyl acrylate and acrylic acid; second, a polyethylene imine aqueous solution is prepared, and the polyethylene imine aqueous solution is added dropwise into a butyl acetate solution of the polylactic acid-polyurethane-polyacrylic acid copolymer, high-speed stirring is conducted, and a water-in-oil emulsion is formed; finally, the water-in-oil emulsion is heated, water is evaporated, a fluorescent solution is formed, and the fluorescent solution is subjected to spray drying to obtain the fluorescent nanoparticles. The nanoparticles of the paper cup are uniform in size, have excellent fluorescent performance and biocompatibility, and have a wide application prospect.

A method for ratiometric detection of putrescine using PtPd NPs@TPE fluorescent nanozymes. This invention first utilizes mesoporous platinum-palladium nanoparticles (PtPd NPs) doped with tetraphenylethylene (TPE) to obtain fluorescent nanoparticles (PtPd NPs@TPE) with oxidase-like activity, which can produce blue fluorescence under 365 nm ultraviolet light excitation. Secondly, this invention utilizes the antioxidant properties of putrescine solution to inhibit the formation of 2,3-diaminophenazine (DAP), an oxidation product, during the oxidation of o-phenylenediamine (OPD) by PtPd NPs@TPE, thereby reducing the DAP content. Based on the relationship between putrescine concentration and the colorimetric intensity of DAP, and the ratio of the yellow fluorescence intensity of DAP at 552 nm to the blue fluorescence intensity of PtPd NPs@TPE at 442 nm, a novel ratiometric fluorescence method for detecting putrescine is constructed. This method can be used to detect actual samples such as dried bean curd, with a detection limit as low as 0.0025 μg / mL.

Provided are fluorescent nanoparticles and their conjugates and methods of using the same for in vivo and in vitro diagnostics and other applications. In some embodiments, provided are fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, provided are methods of manufacturing such nanoparticles. Nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AIEgen™ Bright Green.

The invention discloses a ratio type fluorescent nanometer thermometer based on temperature response type amphiphilic macromolecules and preparation and application of the ratio type fluorescent nanometer thermometer, and belongs to the technical field of fluorescent temperature detection and biomedical detection.The temperature probe is composed of two types of fluorescent nanoparticles with opposite temperature response behaviors, the nanoparticles are formed by self-assembly of temperature-sensitive amphiphilic macromolecules containing aggregation-induced emission molecules, fluorescence of one type of nanoparticles is enhanced due to aggregation of fluorescent molecules induced by collapse of temperature-sensitive polymer chains when the temperature rises, and fluorescence of the other type of nanoparticles is weakened due to de-aggregation or spatial migration of the fluorescent molecules when the temperature rises. Temperature detection is realized by monitoring the change of the intensity ratio of the two fluorescence signals; the temperature probe has high temperature sensitivity and good thermal resolution in a physiological temperature range, and can be used for real-time monitoring of temperature change in living cells.

The invention relates to the technical field of ultrafast preparation of fluorescent nanoparticles, and discloses a fluorescent nanoparticle as well as a preparation method and application thereof. The preparation method comprises the following steps: mechanically emulsifying a mixed solution of an oil phase and a water phase to obtain an oil-in-water emulsion, and reacting under a microwave condition to obtain the fluorescent nanoparticles, wherein the oil phase comprises an initiator, an optional functional monomer and a fluorescent monomer; the water phase comprises an emulsifier, inorganic salt and water. According to the method provided by the invention, different types of small-size fluorescent nanoparticles are prepared in an ultra-fast manner under the assistance of microwaves, and the fluorescent nanoparticles which are uniform in particle size, have the hydration particle size of 50-900 nm and have different modification groups can be prepared; the fluorescent probe has the advantages of being short in reaction time, high in product functionality and excellent in fluorescence intensity performance, AIE and ACQ luminescence is achieved at the same time, the preparation process is simple, green and efficient, and the fluorescent probe can be applied to the fields of fluorescence living imaging, multi-mode imaging, drug delivery, treatment monitoring and the like.

A library of barcoded particle population including combinations of inorganic fluorescent nanoparticles leading to unique identification of the barcoded particle population, and an essay kit including the library of barcoded particle populations and at least one fluorescent protein. Also, a device for biological assay configured to separate at an individual level the composite particles from the assay kit, dispersed in a biological sample and including a reader having at least one illumination source; and a light detector measuring light intensity and coupled to a spectrometer, wherein the at least one illumination source triggers fluorescence of the inorganic fluorescent nanoparticles encapsulated in composite particles and fluorescence of the at least one fluorescent protein

Provided are fluorescent nanoparticles and their conjugates and methods of using the same for in vivo and in vitro diagnostics and other applications. In some embodiments, provided are fluorescent nanoparticles with high solid-state absolute quantum yield. In some embodiments, provided are methods of manufacturing such nanoparticles. Nanoparticles may comprise monomers, such as styrene, and fluorophores, such as AlEgen™ Bright Green.

The invention discloses an invisible two-dimensional code and a preparation method and an identification method thereof, and belongs to the technical field of two-dimensional code anti-counterfeiting. The preparation method comprises the following steps: mixing rare earth doped inorganic fluorescent nanoparticles with the average particle size of 20-50nm and the doping concentration of 2%-4% (molar percentage) with a transparent resin matrix to prepare a solution; forming a two-dimensional code pattern on the substrate through a photoetching and stripping or ink-jet printing process; and finally, curing to form the invisible two-dimensional code film. Through innovative combination of materials and a process, the prepared invisible two-dimensional code is high in light transmittance and invisible under natural light, clear in display and high in recognition rate under excitation of ultraviolet or infrared light, has excellent adhesive force, wear resistance and environmental durability, is particularly suitable for being integrated below a display area of a display panel, and has a wide application prospect. And information hidden storage and high-efficiency anti-counterfeiting are realized while the attractiveness of the equipment is kept.

This application relates to the field of biotechnology and discloses a perovskite-silica composite nanoparticle, its preparation method, and a nucleic acid detection method. The method includes the following steps: preparing a mesoporous silica nanoparticle solution; mixing a precursor solution and the mesoporous silica nanoparticle solution, adding a reaction additive to obtain mesoporous silica nanoparticles loaded with the precursor; washing; first drying, first grinding, and sintering; second grinding, several water washes, and a second drying. The preparation method of the perovskite-silica composite nanoparticles provided in this application produces green fluorescent nanoparticles with ultra-high photostability, solving the problem of poor photostability of traditional perovskite nanocrystals under polar solvent sensitivity and harsh environments (thermal cycling, ultrasound, ultraviolet irradiation, etc.).

The invention discloses a single-molecule fluorescent nanoparticle carrier for protein delivery as well as a screening method and application of the single-molecule fluorescent nanoparticle carrier. The carrier is a compound formed by modifying dipeptide on the surfaces of monomolecular fluorescent nanoparticles (FUNs). The core of the screening method is as follows: firstly, constructing a display library containing nearly 400 different dipeptides on the surface of FUNs through a parallel synthesis technology; then, carrying out high-flux and label-free preliminary screening by calculating aggregation parameters according to the change of an ultraviolet absorption spectrum after the carrier is combined with the protein; and molecular docking software such as Autolock and the like can be combined to evaluate the binding force to carry out auxiliary screening. The invention further provides a method for verifying carrier delivery efficiency and treatment effect by using the cell line and the mouse model. The method is simple in screening process and high in universality, and the obtained vector can efficiently deliver multiple functional proteins such as ovalbumin and CRISPR-Cas9 and shows good application prospects in disease treatment and prevention.

The present application relates to a kind of azide modified fluorescent pi conjugated polymer and the signal rapid amplification and high throughput protein detection imaging method based on DNA modified pi conjugated fluorescent nanoparticles.The present application prepares the oligonucleotide modified fluorescent pi conjugated polymer (OCPNs) based on azide modified fluorescent pi conjugated polymer, the OCPNs has super high fluorescent brightness and extremely high detection sensitivity, without additional fluorescent signal amplification step, different abundance target detection and imaging can be completed;At the same time, in the case of little use, fast, specific targeting effect can be completed, shorten the whole dyeing imaging process, when carrying out multicolor imaging, imaging efficiency is extremely high, has excellent application prospect.

The application discloses fluorescent nanoparticles, protein complexes and immunological detection test strips. In a first aspect, the application provides fluorescent nanoparticles, which comprise AIE ligands and coordinated metal ions. The fluorescent nanoparticles according to the embodiments of the application have at least the following beneficial effects: for the fluorescent nanoparticles formed by the AIE ligands and the coordinated metal ions, when labeling proteins, the proteins can be directly adsorbed to the fluorescent nanoparticles under the action of electrostatic interaction and van der Waals force to be combined to the surface of the fluorescent nanoparticles, thus avoiding the cumbersome labeling steps in the prior art, effectively improving the detection sensitivity, and having good stability and not being easy to be inactivated.

The application belongs to the technical field of non-destructive monitoring of chloride ion concentration in concrete, and relates to a preparation method of a fluorescent probe and a fiber-optic sensing system for monitoring chloride ion concentration. The fluorescent probe is a quartz bare fiber as a carrier, one end of which is plated with a sol-gel film wrapping chloride ion sensitive fluorescent nanoparticles after surface modification, and the other end is connected to a bifurcated optical fiber through a fluorescent probe connector. The other end of the bifurcated optical fiber after being connected to the fluorescent probe is divided into two paths, one of which is connected to a fluorescence spectrometer and then to a computer, and the other of which is connected to a light source. The light emitted by the light source converges into the fluorescent probe through the Y-shaped bifurcated optical fiber. When the fluorescent nanoparticles at the film-plated end of the fluorescent probe contact chloride ions and fluorescence quenching occurs, the degree of fluorescence quenching is received by the fiber-optic spectrometer through the bifurcated optical fiber, and the fluorescence intensity is recorded. The chloride ion concentration is obtained through the Stern-Volmer equation, and the chloride ion concentration monitoring is realized.