Nanometer optical tweezers device and method for accurately controlling nanoparticles and biomolecules

A technology of nanoparticles and biomolecules, which is applied in the field of nano-optical tweezers, can solve the problems of low strength and precision, and achieve the effect of avoiding nanostructures and long nanomanufacturing processes

Inactive Publication Date: 2017-06-27
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In order to achieve the above purpose, the present invention provides a nano-optical tweezers device and method for precisely manipulating nanoparticles and biomolecules, which solves the problem of low capture intensity and precision of traditional optical tweezers existing in the prior art

Method used

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  • Nanometer optical tweezers device and method for accurately controlling nanoparticles and biomolecules
  • Nanometer optical tweezers device and method for accurately controlling nanoparticles and biomolecules
  • Nanometer optical tweezers device and method for accurately controlling nanoparticles and biomolecules

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preparation example Construction

[0067] Concrete preparation method is as follows:

[0068] Step 1.1: Strip the coating layer in the middle of the optical fiber with optical fiber strippers to obtain a bare optical fiber with a length of 1-2 cm and a diameter of 100-125 microns;

[0069] Step 1.2: Put the bare optical fiber prepared in step 1.1 into a glass capillary with an inner diameter of 0.9-1.0 mm and a length of 100-150 mm;

[0070] Step 1.3: Place the bare optical fiber horizontally on the outer flame above the alcohol lamp, and let it stand for 28-38 seconds under the condition of 500-550 degrees. After the optical fiber reaches the melting point, melt the molten part at a speed of 2 mm per second Thinning, when the diameter of the thinning part is reduced from 120-130 microns to 8-10 microns in the length of 1.6-1.8 mm, the drawing speed is increased to 10-15 mm per second to quickly break the optical fiber;

[0071] Step 1.4: Place the broken optical fiber under a microscope for observation. It ca...

Embodiment 1

[0095] Capture and Manipulation of Fluorescent Nanoparticles:

[0096] Process such as figure 2 As shown in (a), when the microlens is not adhered to the fiber tip, such as figure 2 As shown in (a1), the backscattered light signal measured at this time is relatively low; when the microlens is close to and finally adhered to the fiber tip, as shown in figure 2 As shown in (a2), the backscattering signal will first produce a certain jitter, because the adhesion is not stable enough at this time. After a period of time, the backscattering signal rises a step and tends to be stable, which proves that the microlens has been fixed on the fiber.

[0097] A laser with a wavelength of 808 nanometers is passed into the optical fiber, and a single fluorescent nanoparticle with a size of 85 nanometers will be captured by the photon nanojet and cause changes in the backscattered light signal, such as figure 2As shown in (b), the output power from the microlens has been measured to be...

Embodiment 2

[0103] Capture and manipulate DNA molecules:

[0104] Individual biomolecules are more difficult to capture and manipulate than nanoparticles due to their lower refractive index, smaller size, and irregular shape.

[0105] Before being captured, the plasmid DNA molecules were subjected to Brownian motion in the solution. When a laser with a power of 5 milliwatts was passed into the optical fiber, a single DNA molecule was successfully captured. After a period of time, the DNA molecules would undergo Brownian motion and The environment is shaken and released, and the representative picture of the whole process is as follows image 3 (a~c) shown.

[0106] In order to observe DNA molecules with an ordinary optical microscope, we pass a laser with a power of 150 microwatts and a wavelength of 532 nanometers into the optical fiber 2 to irradiate the DNA molecules from the side. After the DNA molecules are irradiated by the laser, they can be seen under the microscope. Green scatt...

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Abstract

The invention discloses a nanometer optical tweezers device and method for accurately controlling nanoparticles and biomolecules. The device includes a microscope, a microflow channel is arranged on an objective table of the microscope, the microflow channel comprises cover glass and a glass slide, two optical fibers are arranged in the microflow channel and are sleeved by glass capillaries, the glass capillaries are fixed on adjustable optical fiber adjusting frames, the other end of one of the optical fibers is connected with a Y type optical fiber coupler, two other arms of the Y type optical fiber coupler are connected with a band-pass filter and a fiber laser, the other end of the band-pass filter is connected with a photoelectric detector, and the other end of the other optical fiber is connected with a laser. The method includes the following specific steps: 1. preparing a parabola-shaped optical fiber tip used for accurate control; 2. fixing a micro lens on the optical fiber tip; 3. using the micro lens assembled in the Step 2 to capture and control fluorescent nanoparticles; and 4. capturing and controlling DNA molecules.

Description

technical field [0001] The invention belongs to the technical field of nano-optical tweezers, and relates to a nano-optical tweezers device method for precisely controlling nanoparticles and biomolecules. Background technique [0002] Traditional optical tweezers is a far-field optical tweezers technology, which uses a high numerical aperture objective lens to converge the laser beam in free space to generate an optical potential well, which can trap and manipulate tiny particles without contact and damage. Powerful tools for research in physical sciences, cell biology, and molecular biology. [0003] In recent years, because the near-field optical tweezers technology can break through the diffraction limit, it has attracted more and more international attention. Existing near-field optical tweezers technologies include plasmonic optical tweezers, slit waveguides, and photonic crystal resonators. These near-field optical tweezers technologies are based on nanostructures su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G21K1/00G02B6/26G02B21/32
CPCG02B6/26G02B21/32G21K1/006
Inventor 李宝军张垚李宇超雷宏香
Owner JINAN UNIVERSITY
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