264 results about "Optical tweezers" patented technology

A real-time manipulating device for microscopic particles comprises a first laser suitable for generating first laser light; the optical tweezer array generation assembly comprises a spatial light modulator which is suitable for modulating the wavefront of the first laser according to the received middle hologram to obtain a first modulated laser and modulating the wavefront of the first laser according to the received target hologram to obtain a second modulated laser; the focusing assembly is suitable for focusing the first modulated laser to generate an intermediate optical tweezers array, and is suitable for focusing the second modulated laser to generate a target optical tweezers array; the real-time processing assembly is suitable for simultaneously determining a plurality of middle holograms according to respective initial positions and respective final positions of the plurality of target microscopic particles, and is also suitable for transmitting the plurality of middle holograms to the optical tweezer array generation assembly according to a time sequence; the real-time processing assembly is also suitable for transmitting the target hologram to the optical tweezers array generation assembly.

The invention discloses a defect-free neutral atom array preparation method and system based on dynamic optical tweezers rearrangement, and belongs to the technical field of quantum computing. The invention discloses a defect-free neutral atom array preparation method and system based on dynamic optical tweezers rearrangement. A static optical tweezers array is generated through a spatial light modulator to capture initial atoms, an optimal rearrangement path is planned by adopting a Hungary algorithm, and dynamic optical tweezers are generated through an acousto-optic deflector to implement atom grabbing, moving and releasing operation. According to the method, a weighted Gerchberg-Saxton algorithm is innovatively combined with real-time image feedback, so that the precise rearrangement of the atom array is realized. Important experimental technical support is provided for quantum calculation and simulation, and the application progress of the neutral atom array in the fields of quantum error correction, fault-tolerant quantum calculation, quantum simulation and the like is effectively promoted.

The invention relates to an aerosol single-droplet optical tweezers detection device and method based on an LED light source, belongs to the technical field of optical capture and particle detection, and solves the problems that a light source adopted by an existing optical tweezers technology is high in cost and large in energy consumption, and large spurious signal interference exists in signal detection. The optical coupling system comprises an LED light source module, a collimating lens, a light beam shaping assembly, a focusing lens assembly and a semi-transparent and semi-reflective mirror assembly which are arranged in sequence. The optical tweezers detection system comprises the light beam coupling system, a single liquid drop suspension system, a gas atmosphere control system, a micro-drop pumping system, a signal detection system and a micro-drop detection device.

The invention relates to the technical field of optical metasurface and light field regulation and control, in particular to a light pressure multi-dimensional regulation and control method and device based on a metasurface microstructure array. The method comprises the following steps: designing and preparing a metasurface microstructure array, wherein the microstructure array is composed of a plurality of sub-wavelength scale three-dimensional microstructure units arranged on a dielectric substrate; surface plasmon resonance is excited by adjusting structural parameters of the three-dimensional microstructure units and adjusting working parameters of a light source acting on the microstructure array, and near-field localization and enhancement are achieved. And calculating, regulating and controlling the light pressure borne by the metasurface microstructure array based on the Maxwell stress tensor. The method can keep a remarkable light pressure enhancing effect in a wide spectrum range of 380-1300 nm, and is suitable for various material systems such as silver, gold and the like. The method can be applied to the fields of optical tweezers systems, optical control devices, precision measurement and the like, and has the advantages of flexible design, simplicity and convenience in preparation, high regulation and control precision and the like.

The invention provides a tip Raman-based metal oxide nanoparticle in-situ detection method, which comprises: S1, dispersing metal oxide nanoparticles in a nanoparticle dispersion liquid, focusing laser to the tip of a nanometer tip through an objective lens, capturing a single nanoparticle by using an optical tweezers technology, and attaching the single nanoparticle to the nanometer tip; s2, focusing Raman laser on a nano needle tip-particle interface, generating a local high-temperature field through a photo-thermal near-field coupling effect, triggering a thermal chemical reaction of nano particles, and synchronously collecting a Raman spectrum; and S3, carrying out pretreatment and characteristic peak analysis on the collected Raman signal of the Raman spectrum, and determining the reaction critical temperature and the dynamic process in combination with temperature calibration data. According to the invention, capture and accurate heating of the nanoparticles are realized through photo-thermal coupling of the optical tweezers and the needle tip, and thermal chemical reaction monitoring of the nanoparticles is realized.

The invention relates to full-dielectric adjustable optical tweezers based on a composite phase superlens, and belongs to the technical field of optical tweezers. Two regulation modes of geometric phase and transmission phase are integrated into a dielectric metasurface periodic structure, and a circular lens surface is combined according to a specific mapping and arrangement mode. The super lens can excite two different output electric fields under the input of linearly polarized light due to the regulation and control of a composite phase, and the two electric fields can independently exist under the input of circularly polarized light with opposite chirality. Therefore, by adjusting the polarization type of the incident light source of the super lens, free switching of multiple optical capturing modes of particles in a field can be realized. Compared with the existing optical tweezers, the control mode can be flexibly adjusted on the premise that the structure of the device is not changed, and more diversified particle capturing requirements can be met. The high-flexibility and easy-to-integrate micro-nano structure can provide powerful guarantee for the performance of a compact composite optical capturing device.

The invention discloses a biological experiment automatic touch-free adding system based on optical control and an operation method thereof, and relates to the technical field of biological experiment equipment, the system comprises the following components: an optical marking module, an optical imaging and detection module, an optical control module and an automatic control module; high automation of biological experiments is realized by integrating a plurality of modules including optical marking, optical imaging and detection, optical control and automatic control, efficient and stable marking of biological samples is ensured by utilizing specific marking of a quantum dot solution and combining accurate control of a microfluidic channel in the optical marking stage, and then, the accuracy of the biological samples is ensured. Through advanced equipment such as a high-resolution fluorescence microscope and an optical spectrum analyzer, the system can accurately obtain optical information of a marked biological sample, a solid foundation is provided for subsequent control and recognition, in the optical control stage, the optical tweezers system achieves accurate control over the biological sample in a touch-free mode, and the accuracy of the biological sample is improved. Pollution and damage possibly caused by traditional physical contact are avoided.

The utility model relates to the technical field of biological tissue engineering, in particular to an organ chip platform integrated with optical tweezers, which is used for assembling and culturing three-dimensional cell tissues and comprises an organ chip with optical transparency and holographic optical tweezers integrated with the organ chip. The organ chip is horizontally arranged and comprises an upper-layer plate, a middle-layer plate and a lower-layer plate which are sequentially stacked, the middle-layer plate is provided with at least two first culture pools used for inducing and differentiating stem cells into different types of cells and at least one second culture pool used for assembling and culturing the different types of cells, and the lower-layer plate is provided with at least two second culture pools used for assembling and culturing the different types of cells. The channel is connected with the first culture pond and the second culture pond; and an objective lens of the holographic optical tweezers faces the top surface or the bottom surface of the organ chip and is used for capturing, moving and assembling cells in the first culture pool or the second culture pool. According to the utility model, the dependence on a micro-pattern processing technology can be reduced, the cost is reduced, and the regulation and control capability on an organoid tissue structure is improved.

The invention relates to the technical field of tissue engineering, in particular to a 3D printing platform integrated with holographic optical tweezers and a bionic tissue construction method.The printing platform comprises a holographic optical tweezers module used for forming an optical trap array in an objective lens focal region of the holographic optical tweezers module; the charging barrel is used for storing biological materials and can move between a storage position and a working position; the printing base can move between the feeding station and the optical tweezer control station; the objective table is installed on the printing base and can move in the horizontal direction and the vertical direction; the light curing device is fixed on the objective table and is used for emitting light to induce the biological material to be cured; the cell culture plate is fixed on the objective table and stacked on the photocuring device; the cell culture plate is provided with a printing chamber and a cell chamber, and the printing chamber and the cell chamber are communicated through a channel. A macroscopic ordered three-dimensional structure can be constructed, and ordering and patterning of microscale organisms in the macroscopic three-dimensional structure are also realized.

The invention discloses a micro-fluidic chip device and an optical tweezers experimental method.The micro-fluidic chip device comprises a micro-fluidic chip, the micro-fluidic chip is provided with at least one inflow channel and at least one outflow channel, the micro-fluidic chip is provided with an optical tweezers control and imaging area, the inflow channel is communicated with the outflow channel through a fluid converging structure, and the outflow channel is communicated with the optical tweezers control and imaging area; the fluid convergence structure is at least partially located in the optical tweezers control and imaging area; the chip holder can be fixed in the optical tweezers system through the mounting structure, the chip holder is further provided with a clamping structure, at least one fluid access port and at least one fluid access port, the micro-fluidic chip is clamped and fixed by the clamping structure, the inflow channel is communicated with the corresponding fluid access port, and the fluid access port is communicated with the corresponding outflow channel. According to the invention, the positioning accuracy of the micro-fluidic chip in an optical tweezers system and the stability of the micro-fluidic chip in an optical tweezers experiment can be ensured, so that the capture efficiency and the imaging effect in the optical tweezers experiment are improved, and the repeatability of a measurement result is improved.

The invention provides a linear optical tweezer astigmatism field regulation and control method based on an optical transmission matrix. The design method comprises the following steps: establishing a 4 * 4 optical matrix for generating a linear optical tweezer astigmatism field and a transmission equation; analyzing the regulation and control rule of the parameters of the optical element on the astigmatism characteristics; collecting a fluorescence slice image of the initial astigmatic light field through an axial tomography scanning method; reconstructing the spatial distribution of the initial image light field; judging whether the light field accords with a preset astigmatism characteristic or not, if not, determining the astigmatism characteristic to be optimized, and adjusting element parameters according to a regulation and control rule; and acquiring the adjusted image of the astigmatism light field again, reconstructing the image, and continuously performing judgment iteration until the image accords with the preset astigmatism characteristic, thereby realizing regulation and control of the astigmatism light field. Matrix optics is used in optimization of a linear optical tweezer image dispersion light field optical system, the limitation of traditional trial and error design is avoided, the method can be applied to the fields of light control technology, light sheet fluorescence microscopic imaging and the like, and the performance of the optical system is improved.

The invention relates to the field of cell analysis, and provides a multi-mode digital micro-fluidic system and method for cell sorting analysis, the system comprises an active digital micro-fluidic platform, the active digital micro-fluidic platform comprises a micro-fluidic chip, an upper computer control system and an FPGA hardware circuit, an upper computer generates a liquid drop path planning instruction, the FPGA hardware circuit converts the liquid drop path planning instruction into an electrode driving signal, and the electrode driving signal is transmitted to the micro-fluidic chip; the nanoliter-grade liquid drops are driven to change between the electrodes; the optical tweezers platform comprises a laser device, an optical microscope and a CMOS camera, laser beams are generated by the laser device to form a three-dimensional optical potential well, the laser beams are transmitted into the micro-fluidic chip through the optical microscope to realize non-contact control on cells, and the CMOS camera records images and transmits the images back to the upper computer; the objective table clamp is used for realizing cooperative control of the optical tweezers and the active digital microfluidics; and the image recognition system is used for recognizing morphological characteristics of cells in the liquid drops based on a convolutional neural network and screening target cells. According to the invention, integration of high-throughput nondestructive cell sorting and in-situ multi-modal analysis is realized.

The invention discloses a special optical tweezers array generation method and system, and the method comprises the steps: employing two acousto-optic deflectors which are perpendicular to each other to sequentially process a laser beam, carrying out the time-sharing scanning modulation or time-sharing scanning and multi-frequency function superposition modulation of the two acousto-optic deflectors, and generating a special optical tweezers array on a two-dimensional plane. According to the invention, the mode of time-sharing scanning modulation or time-sharing scanning and multi-frequency function superposition modulation is adopted to carry out time-sharing scanning modulation on the two acousto-optic deflectors, the optical tweezers array with a special shape is realized, the method is simple to realize, the system required by the realization method is simple in structure and convenient to operate, and the method can be widely applied to various application scenes with special requirements on the optical tweezers array.

The utility model discloses a holographic optical tweezers device for eliminating zero-order diffraction, and the device comprises a laser which is used for emitting a first laser beam; the beam expanding and collimating module is used for controlling the diameter of the first laser beam to obtain a third laser beam; the spatial light modulator is used for carrying out light field modulation on the third laser beam, and the modulated light field is formed after focusing; and the reflection filtering module is used for filtering zero-order diffraction light interference and adjusting a light path. Zero-order diffraction light interference is filtered through the reflection filtering module, meanwhile, the occupied space of an optical path system is effectively reduced, the problems that an optical tweezers system is too large in occupied space and inconvenient to carry in the aspect of optical path construction, an optical path is interfered by zero-order diffraction light, optical path equipment is inconvenient to finely adjust and the like are solved, and the operation process of the optical tweezers is optimized.

The invention provides a petal-shaped homogeneous phase nonlinear rotating beam generation method based on phase regulation and control. By designing a phase distribution function with spiral symmetry and combining dynamic regulation and control of morphological parameters and transmission distance, petal-shaped light beams with different petal numbers, rotation angles and energy distribution can be generated. The light beam shows a homogeneous phase characteristic, high directivity and a stable vortex structure in propagation, is especially suitable for the field of optical tweezers, forms a high-intensity focusing area by adjusting spiral symmetrical phase distribution, and can stably capture micron-sized particles.

The invention provides a multi-focus excitation confocal fluorescence microscopic imaging flow cytometry screening method. The method is characterized in that a laser beam generates three laser beams through a spatial light modulator, and the three laser beams are coherently superposed in a focal plane of a microscope objective to generate a two-dimensional optical lattice light field. And after the other laser beam is shaped by the cylindrical lens, linear laser optical tweezers are realized through the microscope objective, cells to be detected in an imaging detection area of the micro-flow chip are captured, and accurate active optical control on the rotation angle of the cells to be detected is realized. In a cell rotation process, a two-dimensional optical lattice light field rapidly scans in a focal plane at different angles, and a fluorophore in the cell is excited to generate a fluorescence signal. The confocal pinhole array installed at the position conjugate with the focal plane eliminates background fluorescence, a high-spatial-resolution fluorescence image of a three-dimensional structure in the cell is obtained, and cell screening is achieved. The method provided by the invention has the characteristics of high detection rate, high accuracy and the like, and has wide application prospects in the research fields of biology, medicine and life science.

The invention discloses a confocal alignment device and method for correlation beams of double-beam optical tweezers, and belongs to the technical field of optical tweezers. A laser light source of the device is subjected to beam expansion, then passes through a linear polarizer for polarization and a # imgabs0 # wave plate, and then enters a polarization splitting prism for beam splitting; reflected light S of the polarization splitting prism enters a first objective lens after being reflected by a first reflector and a first dichroscope, and emergent light P enters a second objective lens after being reflected by a second dichroscope; the second reflecting mirror is arranged in an S light path passing through the second dichroscope; the plano-convex lens is arranged in front of the imaging camera and is coaxial with the imaging camera; an imaging sheet fixed on the single-axis displacement platform can be moved in and out in a light path; according to the method, an imaging sheet is moved out or introduced between objective lenses which are coaxially mounted, and a light blocking sheet is moved out and introduced in an S light path, so that respective imaging of two beams of light can be realized, the radial space center position of a correlation light beam can be directly observed, and the alignment adjustment efficiency of the light trap is improved.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

The invention relates to a method for controlling a single cell and a single liquid drop based on light, which realizes capture of a target single cell and export of the single liquid drop on a micro-fluidic chip by combining an optical tweezers technology and a photoinduced bubble technology, and comprises the following steps: introducing a sample to be detected into a main channel of the micro-fluidic chip; using an optical tweezers technology to control a target single cell and dragging the target single cell into a side chamber; excluding non-target single cells; replacing liquid to treat the target single cell; a target product forms a single liquid drop by utilizing a photoinduced bubble technology and is guided out. The technical problems that in the prior art, single cells and single liquid drops cannot be accurately controlled at the same time, and separation and culture of intracellular bacteria cannot be achieved are solved.

The invention discloses a high-order mode fiber laser based on a few-mode fiber grating, and belongs to the technical field of optical communication. The fiber laser is composed of a pumping source, a wavelength division multiplexer, a single-mode erbium-doped fiber, a first few-mode long-period fiber grating, a second few-mode long-period fiber grating, a few-mode fiber coupler, a few-mode fiber circulator, a few-mode fiber Bragg grating, a few-mode fiber circulator and a single-mode fiber coupler. Mode conversion is achieved through the few-mode long-period fiber bragg grating, mode reflection and mode separation are achieved through the few-mode fiber bragg grating, and simultaneous output of LP01 mode lasing, LP11 mode lasing and LP21 mode lasing is achieved under a single resonant cavity. The method for outputting the laser by taking the optical fiber as the medium is one of effective methods for realizing optical tweezers, and the high-order mode optical fiber laser based on the few-mode optical fiber grating can output a low-order mode light beam and a high-order mode light beam at the same time, realizes multi-dimensional control of particles, and has a better application prospect in the fields of material science and life science.

The invention relates to an optical fiber optical tweezers probe for multi-particle capture, a preparation method and application. The optical fiber optical tweezer probe comprises a single single-mode optical fiber, one end of the single single-mode optical fiber is a cone-like end, and a black porous carbon-based thermoplastic polyurethane polymer is attached to the surface of the end portion of the cone-like end. The optical fiber optical tweezers probe is manufactured according to the following path: one end of a single single-mode optical fiber is sequentially twisted and subjected to electrode discharge; twisting the other end of the single single-mode optical fiber, placing a thermoplastic polyurethane optical fiber coating in an exposed optical fiber twisting area, and performing tapering treatment, so that the other end of the single single-mode optical fiber is provided with a black porous conical transition area with carbon-based thermoplastic polyurethane polymerization after the treatment. By applying the scheme, when the optical fiber optical tweezers probe is prepared, other additional optical elements and additional experimental materials are not inserted, and low-cost and high-efficiency particle aggregation capture based on the single-mode optical fiber can be realized.

The present application relates to a kind of single bacterial detection methods based on Raman spectroscopy technique through DMD and microfluidic, laser, DMD, microscope, CCD camera, microfluidic chip and Raman spectrum detection analysis platform are used, the method injects the microorganism sample to be measured into microfluidic chip, captures cell by DMD-based optical tweezers technology, collects the Raman spectrum of microorganism cell, processes spectral data, judges whether microorganism exists according to spectral characteristics and its kind, metabolic state information.The present application has the characteristics of non-invasive, high sensitivity, can realize rapid high-throughput single bacterial in situ detection, and has important significance for bacterial detection.

The invention relates to the technical field of organ chips and tissue engineering, in particular to an organ chip platform integrated with optical tweezers and a three-dimensional cell assembling method, the platform comprises an organ chip with optical transparency and an optical tweezers system integrated with the organ chip; the organ chip comprises at least two induced differentiation culture pools and at least one assembly culture pool; the induced differentiation culture pool is used for respectively culturing the stem cells and inducing and differentiating the stem cells into at least two different types of cells; the optical tweezers system is used for capturing cells and moving the cells from the induced differentiation culture pool to the assembly culture pool; assembling the at least two different types of cells into an initial three-dimensional cell tissue according to the proportion, sequence and pattern structure in the target organ tissue in the assembly culture pool; the assembly culture pool is used for culturing the initial three-dimensional cell tissue to obtain the three-dimensional cell tissue with the target pattern structure. The technical threshold of the organ chip can be reduced, and the biological functionality of the organ chip is improved.

The invention provides a pair of novel hollow optical fiber optical tweezers. The device is characterized by comprising a hollow optical fiber and a light source. Experiment sample liquids of different types of cells (particles) are injected into the microfluidic channel 1 of the hollow optical fiber optical tweezers, light emitted by the light source 2 can form an optical tweezers effect at the emergent end through a centrosymmetric fiber core of the hollow optical fiber and capture the cells (particles), and the different cells (particles) in the experiment sample liquids are controlled in the capture area 3 through constraint of light field force. The micro-fluidic chip can be used as a functional module in the micro-fluidic chip, has the functions of capturing, shunting and detecting cells (particles) and the like, is additionally provided with a novel optical fiber photodynamic control device by combining with the structural design of a micro-channel in the chip, and is used for realizing detection and analysis of various medical biological components in different forms; and a new technical basis is provided for early screening of cancers.

The invention discloses an optical fiber optical tweezers system for realizing three-dimensional control of red blood cells in a living blood vessel across the air, and relates to the technical field of photoelectricity, and the system comprises a laser, optical fiber optical tweezers, a six-axis fine adjustment frame, an objective table and an imaging observation assembly. The laser device outputs laser, long-distance focusing is achieved through the optical fiber tweezers with the output end being of a three-section gradual change structure, the focal length is larger than or equal to 30 microns, the focusing point can cross epidermal tissue and a blood vessel wall, an optical potential hydrazine is formed in a living blood vessel, and light gradient force is applied across the air to achieve stable capturing of flowing red blood cells. The optical fiber optical tweezers are adjusted through the six-axis fine adjustment frame, and three-dimensional control over the position of the captured red blood cells can be achieved. The imaging observation assembly carries out real-time imaging, and operation visibility is guaranteed. According to the invention, simultaneous capture and serial assembly can be carried out on a plurality of red blood cells in a living blood vessel. The system has the advantages of compact structure, biocompatibility, stable control and the like, and is expected to provide a powerful tool for function research, microcirculation analysis and the like of red blood cells in living blood vessels.

The invention relates to the field of quantum computing, and particularly discloses a method and device for achieving large-scale extensible neutral atom quantum computing based on a VCSEL array. A plurality of vertical emitting laser VCSEL units with working parameters capable of being independently controlled are used for constructing a high-uniformity dot matrix light source, then a focusing system for focusing laser is constructed, the dot matrix light source is focused, and an optical tweezers array with enough strength and proper size is formed and used for capturing neutral atoms. A larger-scale optical tweezers array can be achieved through the VCSEL array prefabricated through micromachining, the number of dot matrixes is not limited by a high numerical aperture NA field angle, the possibility is provided for quantum calculation to expand to more quantum bits in the future, the expandability has important significance along with continuous increasing of the quantum calculation for the number of quantum bits, and the quantum tweezers array can be applied to a large-scale optical tweezers array. Compared with a high numerical aperture NA lens, a VCSEL array and a micro lens array, the technology is relatively mature, large-scale preparation and integration are easier to realize, the cost is reduced, and popularization and application of the quantum computing technology are facilitated.

The invention relates to the technical field of AI glasses packaging, in particular to an FPC micro-optical module packaging system and method for AI glasses, and the system comprises at least one first optical element, at least one second optical element, an optical tweezers array generation system, an in-situ monitoring and feedback system and a curing system. The invention aims to solve the problems of low alignment precision and poor yield of optical module packaging in the prior art, and provides a micro-optical module precise packaging system and method capable of realizing active, real-time feedback and in-situ self-alignment.

The invention provides a single-frequency laser system and an optical clock and optical tweezers comprising the single-frequency laser system, a first single-frequency laser of the single-frequency laser system is a single-frequency thulium-doped distributed feedback fiber laser, and the first single-frequency laser is used for outputting first single-frequency laser; the thulium-doped optical fiber amplification module is used for receiving the first single-frequency laser and amplifying the power of the first single-frequency laser to form first amplified laser; the second single-frequency laser is used for outputting second single-frequency laser; the erbium-doped fiber amplification module is used for receiving the second single-frequency laser and amplifying the power of the second single-frequency laser to form second amplified laser; the laser beam combining module is used for combining the first amplified laser and the second amplified laser to form combined laser; and the sum frequency module is used for performing sum frequency conversion on the combined laser to form output laser which is output outwards. When the single-frequency laser system is applied to the optical clock, the frequency stability and various performance indexes of the optical clock can be improved; when the method is applied to optical tweezers, the control performance of the optical tweezers on atoms can be improved.

The utility model provides a kind of optical fiber optical tweezers fluorescence lifetime detection equipment, by the light beam emitted by light source is divided into two ways, using the first optical fiber coupler to the two-way light signal received is coupled, and the light signal after coupling is transmitted to the optical fiber circulator connected, transmission to displacement control assembly by optical fiber circulator. Displacement control assembly utilizes one-way light signal to carry out target micro-particle capture, utilizes another light beam corresponding light signal to excite target micro-particle to generate excitation fluorescence signal, utilizes detection assembly to carry out photon number detection to excitation fluorescence signal, determines the fluorescence lifetime of target micro-particle. The utility model equipment can be realized in flexible control micro-particle, and the fluorescence lifetime of micro-particle is detected, and the utility model is more simple and efficient, applicable to precision large instrument to carry out ultrafast signal detection.

This invention discloses a multi-functional optical microscopy imaging system that integrates multiple imaging techniques, including fluorescence lifetime imaging, bright-field microscopy, Raman microscopy, and one sample manipulation technique, optical tweezers, in a single microscope setup. This allows for the acquisition of structural, functional, and molecular information from the sample with simultaneous sample manipulation function. Various design approaches have been outlined for a multi-modality microscopy by segmenting the entire operational wavelength range of an objective lens into separate spectral channels, each dedicated to a specific optical technique.