163 results about "Optical tweezers" patented technology

The invention relates to the technical field of optical metasurface and light field regulation and control, in particular to a light pressure multi-dimensional regulation and control method and device based on a metasurface microstructure array. The method comprises the following steps: designing and preparing a metasurface microstructure array, wherein the microstructure array is composed of a plurality of sub-wavelength scale three-dimensional microstructure units arranged on a dielectric substrate; surface plasmon resonance is excited by adjusting structural parameters of the three-dimensional microstructure units and adjusting working parameters of a light source acting on the microstructure array, and near-field localization and enhancement are achieved. And calculating, regulating and controlling the light pressure borne by the metasurface microstructure array based on the Maxwell stress tensor. The method can keep a remarkable light pressure enhancing effect in a wide spectrum range of 380-1300 nm, and is suitable for various material systems such as silver, gold and the like. The method can be applied to the fields of optical tweezers systems, optical control devices, precision measurement and the like, and has the advantages of flexible design, simplicity and convenience in preparation, high regulation and control precision and the like.

The invention provides a tip Raman-based metal oxide nanoparticle in-situ detection method, which comprises: S1, dispersing metal oxide nanoparticles in a nanoparticle dispersion liquid, focusing laser to the tip of a nanometer tip through an objective lens, capturing a single nanoparticle by using an optical tweezers technology, and attaching the single nanoparticle to the nanometer tip; s2, focusing Raman laser on a nano needle tip-particle interface, generating a local high-temperature field through a photo-thermal near-field coupling effect, triggering a thermal chemical reaction of nano particles, and synchronously collecting a Raman spectrum; and S3, carrying out pretreatment and characteristic peak analysis on the collected Raman signal of the Raman spectrum, and determining the reaction critical temperature and the dynamic process in combination with temperature calibration data. According to the invention, capture and accurate heating of the nanoparticles are realized through photo-thermal coupling of the optical tweezers and the needle tip, and thermal chemical reaction monitoring of the nanoparticles is realized.

The invention relates to full-dielectric adjustable optical tweezers based on a composite phase superlens, and belongs to the technical field of optical tweezers. Two regulation modes of geometric phase and transmission phase are integrated into a dielectric metasurface periodic structure, and a circular lens surface is combined according to a specific mapping and arrangement mode. The super lens can excite two different output electric fields under the input of linearly polarized light due to the regulation and control of a composite phase, and the two electric fields can independently exist under the input of circularly polarized light with opposite chirality. Therefore, by adjusting the polarization type of the incident light source of the super lens, free switching of multiple optical capturing modes of particles in a field can be realized. Compared with the existing optical tweezers, the control mode can be flexibly adjusted on the premise that the structure of the device is not changed, and more diversified particle capturing requirements can be met. The high-flexibility and easy-to-integrate micro-nano structure can provide powerful guarantee for the performance of a compact composite optical capturing device.

The utility model relates to the technical field of biological tissue engineering, in particular to an organ chip platform integrated with optical tweezers, which is used for assembling and culturing three-dimensional cell tissues and comprises an organ chip with optical transparency and holographic optical tweezers integrated with the organ chip. The organ chip is horizontally arranged and comprises an upper-layer plate, a middle-layer plate and a lower-layer plate which are sequentially stacked, the middle-layer plate is provided with at least two first culture pools used for inducing and differentiating stem cells into different types of cells and at least one second culture pool used for assembling and culturing the different types of cells, and the lower-layer plate is provided with at least two second culture pools used for assembling and culturing the different types of cells. The channel is connected with the first culture pond and the second culture pond; and an objective lens of the holographic optical tweezers faces the top surface or the bottom surface of the organ chip and is used for capturing, moving and assembling cells in the first culture pool or the second culture pool. According to the utility model, the dependence on a micro-pattern processing technology can be reduced, the cost is reduced, and the regulation and control capability on an organoid tissue structure is improved.

The invention provides a linear optical tweezer astigmatism field regulation and control method based on an optical transmission matrix. The design method comprises the following steps: establishing a 4 * 4 optical matrix for generating a linear optical tweezer astigmatism field and a transmission equation; analyzing the regulation and control rule of the parameters of the optical element on the astigmatism characteristics; collecting a fluorescence slice image of the initial astigmatic light field through an axial tomography scanning method; reconstructing the spatial distribution of the initial image light field; judging whether the light field accords with a preset astigmatism characteristic or not, if not, determining the astigmatism characteristic to be optimized, and adjusting element parameters according to a regulation and control rule; and acquiring the adjusted image of the astigmatism light field again, reconstructing the image, and continuously performing judgment iteration until the image accords with the preset astigmatism characteristic, thereby realizing regulation and control of the astigmatism light field. Matrix optics is used in optimization of a linear optical tweezer image dispersion light field optical system, the limitation of traditional trial and error design is avoided, the method can be applied to the fields of light control technology, light sheet fluorescence microscopic imaging and the like, and the performance of the optical system is improved.

The invention relates to the field of cell analysis, and provides a multi-mode digital micro-fluidic system and method for cell sorting analysis, the system comprises an active digital micro-fluidic platform, the active digital micro-fluidic platform comprises a micro-fluidic chip, an upper computer control system and an FPGA hardware circuit, an upper computer generates a liquid drop path planning instruction, the FPGA hardware circuit converts the liquid drop path planning instruction into an electrode driving signal, and the electrode driving signal is transmitted to the micro-fluidic chip; the nanoliter-grade liquid drops are driven to change between the electrodes; the optical tweezers platform comprises a laser device, an optical microscope and a CMOS camera, laser beams are generated by the laser device to form a three-dimensional optical potential well, the laser beams are transmitted into the micro-fluidic chip through the optical microscope to realize non-contact control on cells, and the CMOS camera records images and transmits the images back to the upper computer; the objective table clamp is used for realizing cooperative control of the optical tweezers and the active digital microfluidics; and the image recognition system is used for recognizing morphological characteristics of cells in the liquid drops based on a convolutional neural network and screening target cells. According to the invention, integration of high-throughput nondestructive cell sorting and in-situ multi-modal analysis is realized.

The invention provides a multi-focus excitation confocal fluorescence microscopic imaging flow cytometry screening method. The method is characterized in that a laser beam generates three laser beams through a spatial light modulator, and the three laser beams are coherently superposed in a focal plane of a microscope objective to generate a two-dimensional optical lattice light field. And after the other laser beam is shaped by the cylindrical lens, linear laser optical tweezers are realized through the microscope objective, cells to be detected in an imaging detection area of the micro-flow chip are captured, and accurate active optical control on the rotation angle of the cells to be detected is realized. In a cell rotation process, a two-dimensional optical lattice light field rapidly scans in a focal plane at different angles, and a fluorophore in the cell is excited to generate a fluorescence signal. The confocal pinhole array installed at the position conjugate with the focal plane eliminates background fluorescence, a high-spatial-resolution fluorescence image of a three-dimensional structure in the cell is obtained, and cell screening is achieved. The method provided by the invention has the characteristics of high detection rate, high accuracy and the like, and has wide application prospects in the research fields of biology, medicine and life science.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

The present application relates to a kind of single bacterial detection methods based on Raman spectroscopy technique through DMD and microfluidic, laser, DMD, microscope, CCD camera, microfluidic chip and Raman spectrum detection analysis platform are used, the method injects the microorganism sample to be measured into microfluidic chip, captures cell by DMD-based optical tweezers technology, collects the Raman spectrum of microorganism cell, processes spectral data, judges whether microorganism exists according to spectral characteristics and its kind, metabolic state information.The present application has the characteristics of non-invasive, high sensitivity, can realize rapid high-throughput single bacterial in situ detection, and has important significance for bacterial detection.

The invention provides a pair of novel hollow optical fiber optical tweezers. The device is characterized by comprising a hollow optical fiber and a light source. Experiment sample liquids of different types of cells (particles) are injected into the microfluidic channel 1 of the hollow optical fiber optical tweezers, light emitted by the light source 2 can form an optical tweezers effect at the emergent end through a centrosymmetric fiber core of the hollow optical fiber and capture the cells (particles), and the different cells (particles) in the experiment sample liquids are controlled in the capture area 3 through constraint of light field force. The micro-fluidic chip can be used as a functional module in the micro-fluidic chip, has the functions of capturing, shunting and detecting cells (particles) and the like, is additionally provided with a novel optical fiber photodynamic control device by combining with the structural design of a micro-channel in the chip, and is used for realizing detection and analysis of various medical biological components in different forms; and a new technical basis is provided for early screening of cancers.

The invention discloses an optical fiber optical tweezers system for realizing three-dimensional control of red blood cells in a living blood vessel across the air, and relates to the technical field of photoelectricity, and the system comprises a laser, optical fiber optical tweezers, a six-axis fine adjustment frame, an objective table and an imaging observation assembly. The laser device outputs laser, long-distance focusing is achieved through the optical fiber tweezers with the output end being of a three-section gradual change structure, the focal length is larger than or equal to 30 microns, the focusing point can cross epidermal tissue and a blood vessel wall, an optical potential hydrazine is formed in a living blood vessel, and light gradient force is applied across the air to achieve stable capturing of flowing red blood cells. The optical fiber optical tweezers are adjusted through the six-axis fine adjustment frame, and three-dimensional control over the position of the captured red blood cells can be achieved. The imaging observation assembly carries out real-time imaging, and operation visibility is guaranteed. According to the invention, simultaneous capture and serial assembly can be carried out on a plurality of red blood cells in a living blood vessel. The system has the advantages of compact structure, biocompatibility, stable control and the like, and is expected to provide a powerful tool for function research, microcirculation analysis and the like of red blood cells in living blood vessels.

The invention relates to the technical field of AI glasses packaging, in particular to an FPC micro-optical module packaging system and method for AI glasses, and the system comprises at least one first optical element, at least one second optical element, an optical tweezers array generation system, an in-situ monitoring and feedback system and a curing system. The invention aims to solve the problems of low alignment precision and poor yield of optical module packaging in the prior art, and provides a micro-optical module precise packaging system and method capable of realizing active, real-time feedback and in-situ self-alignment.

The invention provides a single-frequency laser system and an optical clock and optical tweezers comprising the single-frequency laser system, a first single-frequency laser of the single-frequency laser system is a single-frequency thulium-doped distributed feedback fiber laser, and the first single-frequency laser is used for outputting first single-frequency laser; the thulium-doped optical fiber amplification module is used for receiving the first single-frequency laser and amplifying the power of the first single-frequency laser to form first amplified laser; the second single-frequency laser is used for outputting second single-frequency laser; the erbium-doped fiber amplification module is used for receiving the second single-frequency laser and amplifying the power of the second single-frequency laser to form second amplified laser; the laser beam combining module is used for combining the first amplified laser and the second amplified laser to form combined laser; and the sum frequency module is used for performing sum frequency conversion on the combined laser to form output laser which is output outwards. When the single-frequency laser system is applied to the optical clock, the frequency stability and various performance indexes of the optical clock can be improved; when the method is applied to optical tweezers, the control performance of the optical tweezers on atoms can be improved.

The utility model provides a kind of optical fiber optical tweezers fluorescence lifetime detection equipment, by the light beam emitted by light source is divided into two ways, using the first optical fiber coupler to the two-way light signal received is coupled, and the light signal after coupling is transmitted to the optical fiber circulator connected, transmission to displacement control assembly by optical fiber circulator. Displacement control assembly utilizes one-way light signal to carry out target micro-particle capture, utilizes another light beam corresponding light signal to excite target micro-particle to generate excitation fluorescence signal, utilizes detection assembly to carry out photon number detection to excitation fluorescence signal, determines the fluorescence lifetime of target micro-particle. The utility model equipment can be realized in flexible control micro-particle, and the fluorescence lifetime of micro-particle is detected, and the utility model is more simple and efficient, applicable to precision large instrument to carry out ultrafast signal detection.

This invention discloses a multi-functional optical microscopy imaging system that integrates multiple imaging techniques, including fluorescence lifetime imaging, bright-field microscopy, Raman microscopy, and one sample manipulation technique, optical tweezers, in a single microscope setup. This allows for the acquisition of structural, functional, and molecular information from the sample with simultaneous sample manipulation function. Various design approaches have been outlined for a multi-modality microscopy by segmenting the entire operational wavelength range of an objective lens into separate spectral channels, each dedicated to a specific optical technique.

The utility model belongs to the technical field of optical instruments, and particularly relates to an optical tweezer optical path module and a cell optimization system. The optical tweezer optical path module comprises a supporting base, a laser head mounting seat and a multi-stage attenuation module. The laser head mounting seat is arranged on the supporting base and is used for mounting a laser emitting head for emitting laser; and the multi-stage attenuation module is arranged on the supporting base and comprises a plurality of reflection attenuation mirror groups which are arranged on the light path at intervals, and each reflection attenuation mirror group is used for attenuating the laser intensity and changing the laser direction. The optical tweezer optical path module provided by the utility model has a multi-attenuation mechanism, and a relatively large attenuation multiple range is easier to realize, so that laser power adjustment in a relatively large range is realized, the laser power dynamic adjustment capability is favorably improved, the laser intensity is finely controlled, and cells are not damaged when various cells are fixed through an optical trap formed by laser.

The invention belongs to the technical field of optics, and relates to a vortex laser capable of regulating spiral chirality. The invention aims to solve the problem that single chiral output cannot be realized due to degeneration of a + / -l topological charge vortex mode in a resonant cavity in the prior art. The vortex laser comprises a pumping source, a gain crystal, a concave mirror, an output coupling mirror and an adjustable scattering sheet arranged in a cavity, a non-periodic SiO2 nano-particle film is arranged on the surface of the scattering sheet, the transverse position of the scattering sheet is adjusted through a two-dimensional translation platform, mode symmetry is broken through differential loss generated by interaction of + l and-l vortex light beams and a random scattering structure, and active, continuous and reversible regulation and control of spiral chirality are achieved. The output coupling mirror is provided with a central micropore to select vortex mode oscillation and output, and astigmatism conversion and CCD detection are matched to accurately judge the chirality of an output light beam. The scheme is compact in structure, high in stability and suitable for the fields of optical tweezers, quantum communication, super-resolution imaging and the like.

The application relates to the technical field of microfluidic chips and optical tweezers, in particular to a novel combination of an optical tweezers and a digital microfluidic chip structure manufacturing method, which comprises an upper chip substrate and a lower chip substrate; the upper chip substrate and the lower chip substrate are matched to form an optical tweezers functional area and a digital microfluidic functional area, and the optical tweezers functional area and the digital microfluidic functional area complete planar processing; the upper chip substrate comprises a hydrophobic layer, an insulating layer, an electrode layer and a glass layer; liquid crystal is arranged between the upper chip substrate and the lower chip substrate, and the liquid crystal contains cells. The application combines the optical tweezers and the digital microfluidic chip structure and the manufacturing method thereof, so that the photoelectric current control chip can be reused, the cost is reduced, the liquid phase separation capacity of small substances in microdroplets is improved, the problems of cell liquid phase separation, fixed time culture and detection can be solved, and the two technologies are not conflicted on the chip.

The invention provides a method for realizing ultra-high-speed translation of a Bessel beam by using double acousto-optic modulation elements, which is characterized in that the displacement in the x-axis direction and the displacement in the y-axis direction are changed at a high speed by quickly switching radio frequencies f1 and f2, so that ultra-high-speed punching on the surface of a workpiece is realized; meanwhile, each hole has excellent morphology consistency due to vertical incidence, so that the problem of hole shape distortion caused by inclined incidence at the scanning edge of a traditional galvanometer is avoided; that is to say, the all-solid-state diffractive optical element is adopted, no mechanical moving part exists, the limitation of mechanical inertia is completely eliminated, the response time of light beam displacement only depends on the change speed of a driving signal and the establishment time of the diffraction effect, the MHz-level control effect can be easily achieved, and the speed is several orders of magnitude faster than that of a traditional galvanometer. The method is especially suitable for application scenes such as laser processing, optical tweezers, confocal microscopic imaging and high-speed optical switches.

The invention discloses a particle directional and fixed-point transport system and method based on asymmetric astigmatism light field regulation and control, and belongs to the technical field of optical micro-control. The system comprises a laser, a cylindrical lens, a first plano-convex lens, a second plano-convex lens, a first reflector, a second reflector and a microscope objective. The cylindrical lens is controlled in an off-axis mode to enable a light beam to be incident vertically in an off-axis mode, and light intensity deviation perpendicular to the optical tweezer line direction is formed on the focal plane of the cylindrical lens. And converting the offset into light intensity gradient distribution along the optical tweezers line direction by using a reflector group, and generating the asymmetric linear optical tweezers after focusing by a microscope objective. The light field generates a directional net scattering force to drive the particles to move unidirectionally along the optical tweezers line direction while maintaining stable capture of the particles. Active modulation devices such as an acousto-optic deflector and a spatial light modulator are not needed, particle directional transportation can be achieved only through cooperative regulation and control of optical elements, and the device has the advantages of being simple in structure, low in cost and high in light field stability and is suitable for biomedical microoperation and particle control in a micro-fluidic chip.

The application discloses an array type photoelectric tweezers chip and a manufacturing method thereof, and belongs to the technical field of micro-nano manipulation and semiconductor devices. The chip comprises a substrate layer, a transistor array structure, a liquid phase manipulation cavity and an upper plate structure. The transistor array structure comprises a plurality of NPN type or PNP type photoelectric transistors arranged in a horizontal array, and a deep trench isolation structure extending longitudinally into the substrate layer is arranged between adjacent units. The light receiving surface of each photoelectric transistor unit faces the liquid phase manipulation cavity, and is not covered by an upper light shielding metal layer. The virtual electrode working mode is adopted, a localized dielectrophoresis electric field is generated through light irradiation, and the accurate capture and manipulation of micro-nano particles in the liquid phase cavity are realized. The application solves the problems of low integration of a traditional optical tweezers chip, obstruction of light transmission caused by a metal light shielding layer and the like, has the advantages of compact structure, sensitive response, programmable array and the like, and can be widely applied to the fields of biological cell manipulation, nano material assembly and micro-fluidic analysis.

The invention relates to a consistency control method, device, equipment and storage medium for holographic optical tweezers, and relates to the technical field of holographic optical tweezers. The consistency control method includes S1, obtaining the position information of the controlled cell, obstacle cells and environmental boundaries, as well as the target position. S2, constructing the desired formation including virtual cells and controlled cells based on the position information. S3, updating the position information and executing the following steps until the controlled cell moves to the target position. S6, obtaining the gravitational parameters and repulsive parameters of the virtual cell based on the target position and the position information. S7, obtaining the expected position of the virtual cell at the next moment based on the gravitational parameters and repulsive parameters. S8, obtaining the total repulsive parameters of the controlled cell based on the position information. S9, obtaining the expected position of the controlled cell at the next moment based on the total repulsive parameters, the expected formation and the expected position of the virtual cell at the next moment. S10, updating the light trap position of the holographic optical tweezers based on the expected position of the controlled cell at the next moment.

The utility model discloses a system for capturing aerosol by optical fiber optical tweezers. The system comprises a laser emitting a laser beam, and an isolator, a beam expander group, a half-wave plate and a polarization beam splitter which are sequentially arranged on a light path of the laser beam, the optical fiber optical tweezers aerosol capturing system further comprises a first optical fiber, a second optical fiber and a capturing chamber, the polarization beam splitter divides the laser beam into a first light beam and a second light beam, the first light beam and the second light beam are coupled into the input ends of the first optical fiber and the second optical fiber respectively, and the output ends of the first optical fiber and the second optical fiber enter the capturing chamber; the capturing chamber is provided with an imaging device and a lighting device for monitoring the capturing process, and the output ends of the first optical fiber and the second optical fiber are located on the two opposite sides of the capturing chamber respectively. The optical fiber optical tweezers aerosol capturing system is compact in structure, convenient to carry and operate, capable of being conveniently integrated with other experimental equipment, high in flexibility, free of expensive optical elements and low in cost.

The application relates to a device and a method for detecting saturated vapor pressure of aerosol single droplets, and belongs to the technical field of saturated vapor pressure detection. The device solves the problem of large detection result error of the existing aerosol single droplet saturated vapor pressure measuring device and method. The single droplet optical tweezers suspension device of the device comprises a sample cell, the sample cell comprises a concentric outer ring wall and an inner ring wall, the outer ring wall is connected with one side of the inner ring wall, a single droplet inlet penetrating the outer ring wall and the inner ring wall is arranged at the connecting position; a plurality of gas inlets and gas outlets penetrating the ring wall and being spaced apart are arranged on the outer ring wall and the inner ring wall, the gas inlets and the gas outlets on the outer ring wall are staggered with the gas inlets and the gas outlets on the inner ring wall; the diameter of the gas inlets and the diameter of the gas outlets are both smaller than the diameter of the single droplet inlet. The device can reduce the error caused by the wall effect on the saturated vapor pressure.

Disclosed are an atomic system and an atomic arrangement method, relating to the technical field of quantum computing. In the atomic system, atoms are captured by using a pair of first optical tweezers and second optical tweezers, respectively, such that a single atom captured by each first optical tweezer is bound to a first layer of an atom array, and an atomic ensemble captured by each second optical tweezer is bound to a second layer of the atom array. The atomic system uses intrinsic dependent array light to rearrange an initially loaded first atom array, and moves atoms in the atomic ensemble at the second layer to vacant first optical tweezers at the first layer, thereby successfully loading physical bits matching first information to a plurality of first optical tweezers. The intrinsic dependent array light moves a plurality of atoms at the second layer in parallel, and the intrinsic dependent array light does not need to move atoms one by one from outside the atom array, and does not need to serially fill vacant first optical tweezers in the atom array, thereby solving the problems of long loading time and low atom loading efficiency of a quantum bit array.

The invention relates to an aerosol optical tweezer formant track identification method and system based on clustering analysis, belongs to the technical field of spectrum formant identification, and solves the problems that multiple formant tracks are difficult to accurately, completely and automatically identify during aerosol optical tweezer spectrum data analysis in the prior art, and the identification accuracy is high. And the subsequent physical parameter inversion precision is influenced. The method comprises the following steps: acquiring multi-frame aerosol spectral data, extracting candidate formants, and constructing a time-peak position data set; according to the time-peak data set, determining an anchoring trajectory by using a dynamic trajectory tracking algorithm; calculating a relative wavelength difference value corresponding to each candidate formant based on the anchoring trajectory; performing clustering analysis on all relative wavelength differences, and determining candidate formant trajectories; a formant trajectory is determined based on the anchoring trajectory and the candidate formant trajectory. The method achieves the automatic and high-precision recognition and reconstruction of a plurality of formant tracks from the complex noisy spectrum data based on the stable physical constraint of the relative distance between formants.

The application discloses an acceleration measurement method and device based on optical suspended microparticle centroid motion quantum state, which utilizes optical tweezers to trap a four-level atom and a nanometer microparticle in an optical cavity simultaneously, the optical cavity is driven by driving laser LC, a stable cavity optical field, i.e., cavity mode, is excited and formed in the cavity; the cavity optical field is coupled with the internal energy level transition of the atom and the centroid motion of the nanometer microparticle simultaneously, and three control lasers LES, LER and LGR are used to drive three energy level transition processes of the atom; by controlling the three control lasers LES, LER and LGR, a rapid adiabatic process and an atomic state inversion process are realized; wherein, in the process of preparing the centroid motion of the nanometer microparticle to a target Fock state, the forward rapid adiabatic process and the atomic state inversion process are iteratively executed in sequence; in the acceleration measurement process, the reverse rapid adiabatic process, the atomic state inversion process and the population transfer process based on a pi pulse are comprehensively utilized. The application can further improve the measurement sensitivity.

The application discloses an electric field tilt detection and correction device and method based on an optical suspension system and belongs to the technical field of optical tweezers. The device reflects and converges a single laser propagating horizontally to form an optical trap capturing a microsphere vertically upward, and enables the suspended microsphere to be stably suspended under high vacuum through a feedback cooling technology; one electrode plate is placed on the upper and lower parts of the suspended microsphere respectively; an alternating voltage is applied to the two parallel electrode plates, the multi-axis displacement table is adjusted according to the power spectrum density signal of the XYZ three-axis movement of the microsphere and the electric field intensity amplitude, so that the posture of the parallel electrode plates is controlled, and the electric field formed by the parallel electrode plates is vertical. The application realizes high-precision electric field tilt detection and correction, is simple and convenient to operate, provides guarantee for higher-precision electric field measurement and weak force value measurement, and has a wide range of application scenarios.

This invention discloses a deep-ultraviolet nanosecond-gated Raman and LIBS detection system and method for suspended soil particulate matter nutrients, belonging to the field of optical and smart agriculture sensor technology. The system includes: a laser ejection sampling unit for in-situ soil ablation to generate aerosols; an optical tweezers unit for capturing and suspending individual soil particles; a deep-ultraviolet laser excitation unit for emitting nanosecond dual-pulse lasers to excite the same particle to generate Raman and LIBS signals; a spatial heterodyne spectral interferometry imaging unit for slitless, high-throughput coaxial signal collection and the formation of interference fringes; and a timing control unit for time-division multiplexing detection of Raman and LIBS signals through nanosecond gating. This invention eliminates matrix effects and achieves highly sensitive in-situ, non-destructive, and synergistic detection of atomic and molecular information in single soil particles. Furthermore, Raman and LIBS are used to distinguish the organic content and total composition of nitrogen, phosphorus, and potassium, enabling precise quantification of soil nutrients.

This invention discloses a long working distance diffraction-limited microscope objective and optical tweezers for optical tweezers. The microscope objective comprises five lens groups from the object side to the image side. The first lens group includes cemented first and second lenses; the second lens group includes cemented third and fourth lenses; the third lens group includes cemented fifth and sixth lenses; the fourth lens group includes cemented seventh and eighth lenses; and the fifth lens group includes ninth and tenth lenses. The first to tenth lenses are respectively a biconcave lens, a meniscus lens with its concave surface facing the image side, a meniscus lens with its concave surface facing the image side, a biconvex lens, a biconvex lens, a meniscus lens with its concave surface facing the object side, a biconvex lens, a biconcave lens, a biconvex single lens, and a meniscus lens with its concave surface facing the image side. This microscope objective has a long working distance, and when a reverse parallel beam is incident on the microscope, the focal point converges outside the microscope, with a front focal length (FFL) less than or equal to -6.345 mm.