97 results about "Optical tweezers" patented technology

The invention relates to the technical field of optical metasurface and light field regulation and control, in particular to a light pressure multi-dimensional regulation and control method and device based on a metasurface microstructure array. The method comprises the following steps: designing and preparing a metasurface microstructure array, wherein the microstructure array is composed of a plurality of sub-wavelength scale three-dimensional microstructure units arranged on a dielectric substrate; surface plasmon resonance is excited by adjusting structural parameters of the three-dimensional microstructure units and adjusting working parameters of a light source acting on the microstructure array, and near-field localization and enhancement are achieved. And calculating, regulating and controlling the light pressure borne by the metasurface microstructure array based on the Maxwell stress tensor. The method can keep a remarkable light pressure enhancing effect in a wide spectrum range of 380-1300 nm, and is suitable for various material systems such as silver, gold and the like. The method can be applied to the fields of optical tweezers systems, optical control devices, precision measurement and the like, and has the advantages of flexible design, simplicity and convenience in preparation, high regulation and control precision and the like.

The invention provides a tip Raman-based metal oxide nanoparticle in-situ detection method, which comprises: S1, dispersing metal oxide nanoparticles in a nanoparticle dispersion liquid, focusing laser to the tip of a nanometer tip through an objective lens, capturing a single nanoparticle by using an optical tweezers technology, and attaching the single nanoparticle to the nanometer tip; s2, focusing Raman laser on a nano needle tip-particle interface, generating a local high-temperature field through a photo-thermal near-field coupling effect, triggering a thermal chemical reaction of nano particles, and synchronously collecting a Raman spectrum; and S3, carrying out pretreatment and characteristic peak analysis on the collected Raman signal of the Raman spectrum, and determining the reaction critical temperature and the dynamic process in combination with temperature calibration data. According to the invention, capture and accurate heating of the nanoparticles are realized through photo-thermal coupling of the optical tweezers and the needle tip, and thermal chemical reaction monitoring of the nanoparticles is realized.

The invention provides a linear optical tweezer astigmatism field regulation and control method based on an optical transmission matrix. The design method comprises the following steps: establishing a 4 * 4 optical matrix for generating a linear optical tweezer astigmatism field and a transmission equation; analyzing the regulation and control rule of the parameters of the optical element on the astigmatism characteristics; collecting a fluorescence slice image of the initial astigmatic light field through an axial tomography scanning method; reconstructing the spatial distribution of the initial image light field; judging whether the light field accords with a preset astigmatism characteristic or not, if not, determining the astigmatism characteristic to be optimized, and adjusting element parameters according to a regulation and control rule; and acquiring the adjusted image of the astigmatism light field again, reconstructing the image, and continuously performing judgment iteration until the image accords with the preset astigmatism characteristic, thereby realizing regulation and control of the astigmatism light field. Matrix optics is used in optimization of a linear optical tweezer image dispersion light field optical system, the limitation of traditional trial and error design is avoided, the method can be applied to the fields of light control technology, light sheet fluorescence microscopic imaging and the like, and the performance of the optical system is improved.

The invention relates to the field of cell analysis, and provides a multi-mode digital micro-fluidic system and method for cell sorting analysis, the system comprises an active digital micro-fluidic platform, the active digital micro-fluidic platform comprises a micro-fluidic chip, an upper computer control system and an FPGA hardware circuit, an upper computer generates a liquid drop path planning instruction, the FPGA hardware circuit converts the liquid drop path planning instruction into an electrode driving signal, and the electrode driving signal is transmitted to the micro-fluidic chip; the nanoliter-grade liquid drops are driven to change between the electrodes; the optical tweezers platform comprises a laser device, an optical microscope and a CMOS camera, laser beams are generated by the laser device to form a three-dimensional optical potential well, the laser beams are transmitted into the micro-fluidic chip through the optical microscope to realize non-contact control on cells, and the CMOS camera records images and transmits the images back to the upper computer; the objective table clamp is used for realizing cooperative control of the optical tweezers and the active digital microfluidics; and the image recognition system is used for recognizing morphological characteristics of cells in the liquid drops based on a convolutional neural network and screening target cells. According to the invention, integration of high-throughput nondestructive cell sorting and in-situ multi-modal analysis is realized.

The invention relates to a Raman activated cell sorting system coupled with micro-fluidic and Raman optical tweezers and a method thereof, and relates to the technical field of cell sorting, the system comprises a micro-fluidic chip with low Raman and fluorescence background noise and a pneumatic micro-valve; in the micro-fluidic chip, a sample channel and a micro-cavity are connected with a main channel, and a flow guide channel is connected with the micro-cavity; the flow guide channel and the collecting channel are connected through a pneumatic micro valve. After the pneumatic micro valve is closed, the fluid in the micro cavity is static, and a narrow sample flow is formed in the main channel. And when the Raman collection time is less than the time difference of two adjacent cells flowing through the capturing point of the optical tweezers in the narrow sample flow, high-flux Raman detection can be performed in the narrow sample flow. On the contrary, the target cells can be moved out of the narrow sample flow, and high-precision Raman detection in a long Raman collection scene can be realized. After sorting is completed, fluid flowing of the main channel is stopped, the pneumatic micro valve is opened, the cells stored in the micro cavity are released and collected in a unified mode, the cell suspension volume is more stable and uniform, and follow-up experiment operation is facilitated.

The utility model provides a kind of optical fiber optical tweezers fluorescence lifetime detection equipment, by the light beam emitted by light source is divided into two ways, using the first optical fiber coupler to the two-way light signal received is coupled, and the light signal after coupling is transmitted to the optical fiber circulator connected, transmission to displacement control assembly by optical fiber circulator. Displacement control assembly utilizes one-way light signal to carry out target micro-particle capture, utilizes another light beam corresponding light signal to excite target micro-particle to generate excitation fluorescence signal, utilizes detection assembly to carry out photon number detection to excitation fluorescence signal, determines the fluorescence lifetime of target micro-particle. The utility model equipment can be realized in flexible control micro-particle, and the fluorescence lifetime of micro-particle is detected, and the utility model is more simple and efficient, applicable to precision large instrument to carry out ultrafast signal detection.

This invention discloses a multi-functional optical microscopy imaging system that integrates multiple imaging techniques, including fluorescence lifetime imaging, bright-field microscopy, Raman microscopy, and one sample manipulation technique, optical tweezers, in a single microscope setup. This allows for the acquisition of structural, functional, and molecular information from the sample with simultaneous sample manipulation function. Various design approaches have been outlined for a multi-modality microscopy by segmenting the entire operational wavelength range of an objective lens into separate spectral channels, each dedicated to a specific optical technique.

The utility model belongs to the technical field of optical instruments, and particularly relates to an optical tweezer optical path module and a cell optimization system. The optical tweezer optical path module comprises a supporting base, a laser head mounting seat and a multi-stage attenuation module. The laser head mounting seat is arranged on the supporting base and is used for mounting a laser emitting head for emitting laser; and the multi-stage attenuation module is arranged on the supporting base and comprises a plurality of reflection attenuation mirror groups which are arranged on the light path at intervals, and each reflection attenuation mirror group is used for attenuating the laser intensity and changing the laser direction. The optical tweezer optical path module provided by the utility model has a multi-attenuation mechanism, and a relatively large attenuation multiple range is easier to realize, so that laser power adjustment in a relatively large range is realized, the laser power dynamic adjustment capability is favorably improved, the laser intensity is finely controlled, and cells are not damaged when various cells are fixed through an optical trap formed by laser.

The invention belongs to the technical field of optics, and relates to a vortex laser capable of regulating spiral chirality. The invention aims to solve the problem that single chiral output cannot be realized due to degeneration of a + / -l topological charge vortex mode in a resonant cavity in the prior art. The vortex laser comprises a pumping source, a gain crystal, a concave mirror, an output coupling mirror and an adjustable scattering sheet arranged in a cavity, a non-periodic SiO2 nano-particle film is arranged on the surface of the scattering sheet, the transverse position of the scattering sheet is adjusted through a two-dimensional translation platform, mode symmetry is broken through differential loss generated by interaction of + l and-l vortex light beams and a random scattering structure, and active, continuous and reversible regulation and control of spiral chirality are achieved. The output coupling mirror is provided with a central micropore to select vortex mode oscillation and output, and astigmatism conversion and CCD detection are matched to accurately judge the chirality of an output light beam. The scheme is compact in structure, high in stability and suitable for the fields of optical tweezers, quantum communication, super-resolution imaging and the like.

The invention provides a method for realizing ultra-high-speed translation of a Bessel beam by using double acousto-optic modulation elements, which is characterized in that the displacement in the x-axis direction and the displacement in the y-axis direction are changed at a high speed by quickly switching radio frequencies f1 and f2, so that ultra-high-speed punching on the surface of a workpiece is realized; meanwhile, each hole has excellent morphology consistency due to vertical incidence, so that the problem of hole shape distortion caused by inclined incidence at the scanning edge of a traditional galvanometer is avoided; that is to say, the all-solid-state diffractive optical element is adopted, no mechanical moving part exists, the limitation of mechanical inertia is completely eliminated, the response time of light beam displacement only depends on the change speed of a driving signal and the establishment time of the diffraction effect, the MHz-level control effect can be easily achieved, and the speed is several orders of magnitude faster than that of a traditional galvanometer. The method is especially suitable for application scenes such as laser processing, optical tweezers, confocal microscopic imaging and high-speed optical switches.

The application discloses an array type photoelectric tweezers chip and a manufacturing method thereof, and belongs to the technical field of micro-nano manipulation and semiconductor devices. The chip comprises a substrate layer, a transistor array structure, a liquid phase manipulation cavity and an upper plate structure. The transistor array structure comprises a plurality of NPN type or PNP type photoelectric transistors arranged in a horizontal array, and a deep trench isolation structure extending longitudinally into the substrate layer is arranged between adjacent units. The light receiving surface of each photoelectric transistor unit faces the liquid phase manipulation cavity, and is not covered by an upper light shielding metal layer. The virtual electrode working mode is adopted, a localized dielectrophoresis electric field is generated through light irradiation, and the accurate capture and manipulation of micro-nano particles in the liquid phase cavity are realized. The application solves the problems of low integration of a traditional optical tweezers chip, obstruction of light transmission caused by a metal light shielding layer and the like, has the advantages of compact structure, sensitive response, programmable array and the like, and can be widely applied to the fields of biological cell manipulation, nano material assembly and micro-fluidic analysis.

Disclosed are an atomic system and an atomic arrangement method, relating to the technical field of quantum computing. In the atomic system, atoms are captured by using a pair of first optical tweezers and second optical tweezers, respectively, such that a single atom captured by each first optical tweezer is bound to a first layer of an atom array, and an atomic ensemble captured by each second optical tweezer is bound to a second layer of the atom array. The atomic system uses intrinsic dependent array light to rearrange an initially loaded first atom array, and moves atoms in the atomic ensemble at the second layer to vacant first optical tweezers at the first layer, thereby successfully loading physical bits matching first information to a plurality of first optical tweezers. The intrinsic dependent array light moves a plurality of atoms at the second layer in parallel, and the intrinsic dependent array light does not need to move atoms one by one from outside the atom array, and does not need to serially fill vacant first optical tweezers in the atom array, thereby solving the problems of long loading time and low atom loading efficiency of a quantum bit array.

The invention relates to an aerosol optical tweezer formant track identification method and system based on clustering analysis, belongs to the technical field of spectrum formant identification, and solves the problems that multiple formant tracks are difficult to accurately, completely and automatically identify during aerosol optical tweezer spectrum data analysis in the prior art, and the identification accuracy is high. And the subsequent physical parameter inversion precision is influenced. The method comprises the following steps: acquiring multi-frame aerosol spectral data, extracting candidate formants, and constructing a time-peak position data set; according to the time-peak data set, determining an anchoring trajectory by using a dynamic trajectory tracking algorithm; calculating a relative wavelength difference value corresponding to each candidate formant based on the anchoring trajectory; performing clustering analysis on all relative wavelength differences, and determining candidate formant trajectories; a formant trajectory is determined based on the anchoring trajectory and the candidate formant trajectory. The method achieves the automatic and high-precision recognition and reconstruction of a plurality of formant tracks from the complex noisy spectrum data based on the stable physical constraint of the relative distance between formants.

The application discloses an acceleration measurement method and device based on optical suspended microparticle centroid motion quantum state, which utilizes optical tweezers to trap a four-level atom and a nanometer microparticle in an optical cavity simultaneously, the optical cavity is driven by driving laser LC, a stable cavity optical field, i.e., cavity mode, is excited and formed in the cavity; the cavity optical field is coupled with the internal energy level transition of the atom and the centroid motion of the nanometer microparticle simultaneously, and three control lasers LES, LER and LGR are used to drive three energy level transition processes of the atom; by controlling the three control lasers LES, LER and LGR, a rapid adiabatic process and an atomic state inversion process are realized; wherein, in the process of preparing the centroid motion of the nanometer microparticle to a target Fock state, the forward rapid adiabatic process and the atomic state inversion process are iteratively executed in sequence; in the acceleration measurement process, the reverse rapid adiabatic process, the atomic state inversion process and the population transfer process based on a pi pulse are comprehensively utilized. The application can further improve the measurement sensitivity.

This invention discloses a deep-ultraviolet nanosecond-gated Raman and LIBS detection system and method for suspended soil particulate matter nutrients, belonging to the field of optical and smart agriculture sensor technology. The system includes: a laser ejection sampling unit for in-situ soil ablation to generate aerosols; an optical tweezers unit for capturing and suspending individual soil particles; a deep-ultraviolet laser excitation unit for emitting nanosecond dual-pulse lasers to excite the same particle to generate Raman and LIBS signals; a spatial heterodyne spectral interferometry imaging unit for slitless, high-throughput coaxial signal collection and the formation of interference fringes; and a timing control unit for time-division multiplexing detection of Raman and LIBS signals through nanosecond gating. This invention eliminates matrix effects and achieves highly sensitive in-situ, non-destructive, and synergistic detection of atomic and molecular information in single soil particles. Furthermore, Raman and LIBS are used to distinguish the organic content and total composition of nitrogen, phosphorus, and potassium, enabling precise quantification of soil nutrients.

This invention discloses a long working distance diffraction-limited microscope objective and optical tweezers for optical tweezers. The microscope objective comprises five lens groups from the object side to the image side. The first lens group includes cemented first and second lenses; the second lens group includes cemented third and fourth lenses; the third lens group includes cemented fifth and sixth lenses; the fourth lens group includes cemented seventh and eighth lenses; and the fifth lens group includes ninth and tenth lenses. The first to tenth lenses are respectively a biconcave lens, a meniscus lens with its concave surface facing the image side, a meniscus lens with its concave surface facing the image side, a biconvex lens, a biconvex lens, a meniscus lens with its concave surface facing the object side, a biconvex lens, a biconcave lens, a biconvex single lens, and a meniscus lens with its concave surface facing the image side. This microscope objective has a long working distance, and when a reverse parallel beam is incident on the microscope, the focal point converges outside the microscope, with a front focal length (FFL) less than or equal to -6.345 mm.

The invention relates to an aerosol optical tweezer formant trajectory identification method and system based on iterative clustering, belongs to the technical field of spectrum formant identification, and solves the problem that in the prior art, when aerosol spectrum data with a very long time sequence or extremely high data point density is analyzed, the spectrum data is not accurate. And the problems of difficult trajectory tracking and large computing resource consumption caused by noise and baseline drift are solved. The method comprises the following steps: acquiring multi-frame aerosol spectrum data, and constructing a time-peak position data set; according to the time-peak data set, based on a segmented anchoring rule, determining at least one anchoring trajectory and a corresponding time-peak data subset by adopting a dynamic trajectory tracking algorithm; based on the anchoring trajectory, calculating a relative wavelength difference value, carrying out clustering analysis, and determining a corresponding segmented trajectory; a formant trajectory is determined based on the anchoring trajectory and the segmentation trajectory. The method achieves the automatic and high-precision recognition of a plurality of formant tracks of long-sequence spectral data based on the stable physical constraint of the relative distance between formants.

Embodiments of the present application disclose a single-atom manipulation system, a control method and an electronic device. The single-atom manipulation system comprises: an atomic cooling device configured to form an MOT atomic group, wherein the MOT atomic group is capable of generating atomic fluorescence of a first target wavelength; an atomic trapping device, wherein the atomic trapping device comprises a laser source and a strong focusing lens, the laser source emits a target laser beam of a second target wavelength, the target laser beam passes through the strong focusing lens to form an optical tweezer array to trap the MOT atomic group; and an imaging device, wherein the imaging device is sequentially provided with a filter, a noise fluorescence shielding device, a plano-convex lens and an imaging unit in a reflection direction of the focused atomic fluorescence, and the noise fluorescence shielding device is configured to shield noise fluorescence generated by the atomic cooling device.

A laser control device controls the phase of light output from a spatial light modulator that generates optical tweezers on the basis of the vibration frequency of particles captured by an optical tweezers array.

The invention relates to a double-droplet optical tweezers spontaneous / stimulated Raman spatial resolution measurement device and method based on a confocal system, belongs to the technical field of aerosol microdroplet spectrum trace analysis, and solves the problems that an existing aerosol double-droplet detection device generally depends on a traditional single-beam optical system, cannot capture double droplets, is insufficient in spatial resolution, cannot capture the double droplets, and cannot capture the double droplets. And high-precision spatial resolution is difficult to realize for capturing and dynamic monitoring of the double liquid drops. The device comprises a double-droplet suspension system, an environmental atmosphere control system, an optical path coupling system, a dynamic signal processing system, a droplet imaging system and a confocal optical system. The environment atmosphere control system is communicated with the double-droplet suspension system, and the confocal optical system is respectively communicated with the double-droplet suspension system, the dynamic signal processing system, the droplet imaging system and the light path coupling system; the confocal optical system comprises a microscope objective and a long-wave reflecting short-wave pass mirror, and the microscope objective is arranged between the long-wave reflecting short-wave pass mirror and the double-droplet suspension system.

The invention relates to a metasurface design method for generating an optical tweezers array and a metasurface, the metasurface comprises a plurality of cells arranged in an array shape, and the metasurface design method comprises the following steps: according to target light intensity distribution of the optical tweezers array at a target plane, determining the target light intensity distribution of the optical tweezers array at the target plane; determining the phase shift of each cell forming the metasurface on the light emitted into the metasurface; and determining parameters of each cell according to the phase deviation required to be caused by each cell.

The present application relates to sperm movement technical field, especially a kind of method and device for measuring sperm head rotation frequency, comprising: S1, the image information of sample to be measured is obtained, based on the image information, select target sperm;S2, based on the target sperm, the light spot of the target sperm head after the target sperm is captured to head in the preset optical tweezers light beam is obtained;S3, based on the light spot of the target sperm head, the light spot intensity variation information is obtained, the light spot intensity variation information is converted into electrical signal data, the electrical signal data is carried out power spectrum analysis, and the rotation frequency of the target sperm head is obtained.The present application carries out real-time monitoring and analysis to sperm movement characteristics, can quickly obtain sperm movement characteristics, and the accuracy of measurement result is high.

The invention provides an optical tweezer generation method and device, electronic equipment and a storage medium, and the method comprises the steps: initializing an optical tweezer phase based on a designated optical tweezer phase and an optical tweezer position mask matrix, and initializing an optical tweezer amplitude based on a target optical tweezer amplitude diagram; and updating the phase of the spatial light modulator based on the phase of the optical tweezers and the amplitude of the optical tweezers, respectively updating the phase of the optical tweezers and the amplitude of the optical tweezers based on the phase of the spatial light modulator, and carrying out phase constraint on the updated phase of the optical tweezers based on the appointed phase of the optical tweezers and the position mask matrix of the optical tweezers. Updating the number of iterations and returning to update the phase of the spatial light modulator based on the new optical tweezer phase and the optical tweezer amplitude until the number of iterations reaches an iteration threshold value; and generating the optical tweezers based on the phase of the spatial light modulator. According to the method, the device, the electronic equipment and the storage medium provided by the invention, the global optimization capability is solved by utilizing the iterative algorithm, the phase of the optical tweezers is ensured to be controllable, and the risk of random jumping of the phase of the optical tweezers is avoided.

The invention discloses an angular velocity measuring device based on an optical suspension harmonic oscillator, which belongs to the field of inertial measurement technology and optical tweezers technology, and comprises an imaging camera, a first band-pass filter, a first non-polarization splitting prism, a first objective lens, a second objective lens and a coaxial light source which are coaxially arranged in sequence, the first objective lens and the second objective lens are both mounted in the vacuum cavity and are confocal; the emergent direction of the capture laser is perpendicular to the gravity direction, and a first dichroscope is mounted at the intersection point of the emergent direction of the capture laser and the z-axis; the emitting direction of the modulated laser is the x-axis positive direction and is coplanar with the z-axis; the center of the first non-polarization splitting prism is arranged at the intersection point of the z-axis and the incident direction of the modulated laser; the second dichroscope is arranged between the coaxial light source and the vacuum cavity; and the second band-pass optical filter and the four-quadrant detector are sequentially arranged in the x-axis negative direction of the second dichroscope. According to the invention, high vacuum environment capture and angular velocity measurement of the optical suspension harmonic oscillator are realized.

The invention relates to dynamic adjustable optical tweezers based on a polarization-sensitive transverse bifocal superlens, and belongs to the field of optical tweezers technology and micro-nano optics. According to the super-lens, dynamic regulation and control of a focus are realized by using sensitive response of the silicon nano-column array to polarization through a composite propagation phase and a geometric phase. Under linear polarization or elliptically polarized light, the system can generate transversely symmetrical double focuses; under the circularly polarized light, a single-side focus is formed. By adjusting the polarization state of incident light, a double-particle capture mode, a single-particle capture mode and a particle transverse transport mode can be flexibly switched. According to the invention, the physical structure does not need to be changed, the dynamic control can be realized only by external optical regulation and control, the structure is compact, the integration degree is high, the control is flexible and the like, and a new technical approach is provided for an on-chip particle control system.

The present invention relates to the technical field of tissue engineering, and in particular to a 3D printing platform integrated with holographic optical tweezers, and a bionic tissue construction method. The printing platform comprises: a holographic optical tweezer module configured to form an optical trap array in a focal region of an objective lens of the holographic optical tweezer module; material barrels configured to store biological materials and capable of moving between a storage position and a working position; a printing base capable of moving between a material loading station and an optical tweezer manipulation station; an object stage mounted on the printing base and capable of moving in horizontal and vertical directions; a photocuring device fixed on the object stage and configured to emit light to induce the biological materials to be cured; and a cell culture plate fixed on the object stage and stacked on the photocuring device, wherein the cell culture plate has a printing chamber and a cell chamber, and the printing chamber and the cell chamber are in communication with each other by means of a channel. A macroscopic ordered three-dimensional structure can be constructed, and micro-scale organisms inside the macroscopic three-dimensional structure are also ordered and patterned.

The invention belongs to the technical field of quantum physics and quantum, and particularly relates to an AOD optical tweezers array automatic correction method and system based on a neural network, and the method comprises the following steps: generating an initial AOD optical tweezers array through an acousto-optic deflector; acquiring an image of the initial AOD optical tweezers array, and preprocessing the image; inputting the preprocessed image into a first neural network and a second neural network; calculating a global frequency compensation amount according to the average position deviation and a pre-calibrated frequency-position relation factor; generating an intensity compensation table covering a full frequency domain through an interpolation method based on the intensity gain compensation factors of the optical tweezers; and updating the driving frequency of the AOD according to the global frequency compensation amount, updating the driving amplitude of each channel of the AOD according to the intensity compensation table, and outputting the corrected AOD optical tweezers array. By integrating the neural network model and the closed-loop control process, the full automation of the position and intensity correction of the optical tweezers array is realized.

The invention discloses an atom system and an atom arrangement method, and relates to the technical field of quantum computing. The atom system captures atoms by adopting first optical tweezers and second optical tweezers which are paired one by one, so that single atoms captured by the first optical tweezers are bound to a first layer of the atom array, and atom ensembles captured by the second optical tweezers are bound to a second layer of the atom array. The atomic system rearranges the initially loaded first atomic array by using internal state dependent array light, and moves atoms in the atomic ensemble in the second layer to the first optical tweezers vacant in the first layer, thereby successfully loading the physical bits matched with the first information to the plurality of first optical tweezers. Wherein the internal state dependent array light moves a plurality of atoms in the second layer in parallel, the internal state dependent array light does not need to move atoms from the outside of the atom array one by one, and does not need to serially fill the vacant first optical tweezers in the atom array, so that the problems that the quantum bit array is relatively long in loading time and relatively low in atom loading efficiency are solved.

The invention provides a micro-fluidic chip combining in-situ Raman detection with optical tweezers sorting and a preparation method, and belongs to the technical field of micro-fluidic chips and optical detection.The micro-fluidic chip sequentially comprises a top cover layer, a middle flow channel layer and a base layer from top to bottom, and a preset micro-flow channel network is machined in the middle flow channel layer through femtosecond laser; comprising a cell injection channel, a cell sample loading channel and a sorting outlet channel, one end of the cell sample loading channel is horizontally connected and communicated with one end of the sorting outlet channel, a third conduction channel is led out from one side of a joint part of the cell sample loading channel and the sorting outlet channel and is communicated with one end of a Raman spectrum detection and optical tweezers sorting area, and the other end of the Raman spectrum detection and optical tweezers sorting area is communicated with one end of the cell injection channel; the device has the characteristics of excellent in-situ detection capability, efficient and accurate sorting function, low cost and easiness in manufacturing, and is very suitable for standardized production, popularization and application.

The utility model relates to a coupling module and a confocal microscope-optical tweezers integrated system, and relates to the technical field of optical instruments, and the coupling module comprises a housing which is provided with an installation space which longitudinally penetrates through the housing; a light through hole is formed in the side wall of the mounting space; the dichroscope frame is horizontally and linearly connected in the mounting space in a sliding manner and is aligned with the light through hole; the optical filter frame is located in the installation space and installed on the dichroscope frame. A dichroscope frame and an optical filter frame in the coupling module can slide, so that the optical tweezers module optical path and the confocal microscope optical path can be quickly coupled and separated.