The invention relates to the technical field of determination of enzymes or microorganisms, in particular to a single-molecule
fluorescence detection device which comprises a shell, a detection main body, a display screen and a cleaning
assembly, the detection main body comprises a reactor module, a pipeline module, an adjusting module, an
optical module, a temperature early warning module, a detection module and an image
data processing module; the reactor module fixes a large number of primer
DNA sequences; the pipeline module is used for adding each component of the extension
reaction system into the reactor module for sequencing circulation such as
DNA chain extension and breakage, after the
DNA chain is extended, specific basic groups participating in extension are detected through a
total internal reflection microscope, then
fluorescein is removed, next extension is carried out, and multiple sequencing circulation is completed; data obtained through the
optical module are processed through the image
data processing module in the computer, finally sequence information of the DNA chain to be detected is obtained, and when the temperature of the reactor module is abnormal, the temperature early warning module discovers the temperature
abnormality in time and gives an alarm.